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https://www.readbyqxmd.com/read/29161703/expression-profile-of-notch3-in-mouse-spermatogonia
#1
Ryu Okada, Megumi Fujimagari, Eri Koya, Yoshikazu Hirose, Tomomi Sato, Yukio Nishina
Stable and sustainable spermatogenesis is supported by the strict regulation of self-renewal and differentiation of spermatogonial stem cells (SSC), which are a rare population of undifferentiated spermatogonia. It has been revealed that some signaling factors regulate the self-renewal of SSC; however, the molecular mechanism of SSC maintenance is still not completely understood. Notch signaling is an evolutionarily conserved juxtacrine signaling that plays important roles in the cell fate determination of various tissue stem cells...
January 22, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29157197/selection-for-female-traits-of-high-fertility-affects-male-reproductive-performance-and-alters-the-testicular-transcriptional-profile
#2
Marten Michaelis, Alexander Sobczak, Dirk Koczan, Martina Langhammer, Norbert Reinsch, Jennifer Schoen, Joachim M Weitzel
BACKGROUND: Many genes important for reproductive performance are shared by both sexes. However, fecundity indices are primarily based on female parameters such as litter size. We examined a fertility mouse line (FL2), which has a considerably increased number of offspring and a total litter weight of 180% compared to a randomly bred control line (Ctrl) after more than 170 generations of breeding. In the present study, we investigated whether there might be a parallel evolution in males after more than 40 years of breeding in this outbred mouse model...
November 21, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29155017/p-glycoprotein-mdr1-abcb1-and-breast-cancer-resistance-protein-bcrp-abcg2-affect-brain-accumulation-and-intestinal-disposition-of-encorafenib-in-mice
#3
Jing Wang, Changpei Gan, Rolf W Sparidans, Els Wagenaar, Stéphanie van Hoppe, Jos H Beijnen, Alfred H Schinkel
Encorafenib (LGX818) is a promising BRAF(V600E) inhibitor that has efficacy against metastatic melanoma. To better understand its pharmacokinetics, we studied its interactions with the multidrug efflux transporters ABCB1 and ABCG2 and the multidrug metabolizing enzyme CYP3A. In polarized MDCK-II cells, encorafenib was efficiently transported by canine and human ABCB1 and ABCG2 and by mouse Abcg2. Upon oral administration to wild-type, Abcb1a/1b(-/-), Abcg2(-/-), and Abcb1a/1b;Abcg2(-/-) mice, encorafenib was absorbed very quickly and to very high plasma levels, but without clear changes in oral availability between the strains...
November 14, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29145650/loss-of-p300-and-cbp-disrupts-histone-acetylation-at-the-mouse-sry-promoter-and-causes-xy-gonadal-sex-reversal
#4
Gwenn-Aël Carré, Pam Siggers, Marilena Xipolita, Paul Brindle, Beat Lutz, Sara Wells, Andy Greenfield
CREB-binding protein (CBP, CREBBP, KAT3A) and its closely related paralogue p300 (EP300, KAT3B), together termed p300/CBP, are histone/lysine acetyl-transferases that control gene expression by modifying chromatin-associated proteins. Here, we report roles for both of these chromatin-modifying enzymes in mouse sex determination, the process by which the embryonic gonad develops into a testis or an ovary. By targeting gene ablation to embryonic gonadal somatic cells using an inducible Cre line, we show that gonads lacking either gene exhibit major abnormalities of XY gonad development at 14...
November 14, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29138255/complex-history-and-differentiation-patterns-of-the-t-haplotype-a-mouse-meiotic-driver
#5
Reka K Kelemen, Beatriz Vicoso
The t-haplotype, a mouse meiotic driver found on chromosome 17, has been a model for autosomal segregation distortion for close to a century, but several questions remain regarding its biology and evolutionary history. A recently published set of population genomics resources for wild mice includes several individuals heterozygous for the t-haplotype, which we use to characterize this selfish element at the genomic and transcriptomic level. Our results show that large sections of the t-haplotype have been replaced by standard homologous sequences, possibly due to occasional events of recombination, and that this complicates the inference of its history...
November 14, 2017: Genetics
https://www.readbyqxmd.com/read/29129619/transcriptional-profiling-validates-involvement-of-extracellular-matrix-and-proteinases-genes-in-mouse-gonad-development
#6
Rafal P Piprek, Michal Kolasa, Dagmara Podkowa, Malgorzata Kloc, Jacek Z Kubiak
Extracellular matrix (ECM) plays an important scaffolding role in the establishment of organs structure during development. A great number of ECM components and enzymes (proteinases) regulating formation/degradation of ECM during organ remodeling have been identified. In order to study the role of ECM in the mouse gonad development, especially during sexual differentiation of the gonads when the structure of the testis and ovary becomes established, we performed a global analysis of transcriptome in three main cell types of developing gonad (supporting, interstitial/stromal and germ cells) using transgenic mice, cell sorting and microarray...
November 9, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/29126923/clock-interacts-with-ranbp9-and-is-involved-in-alternative-splicing-in-spermatogenesis
#7
Junbao Yang, Zhiwei Zhang, Yingying Zhang, Xulei Zheng, Yilu Lu, Dachang Tao, Yunqiang Liu, Yongxin Ma
The core circadian gene CLOCK plays an important role in regulating male reproduction. However, the underlying mechanism still remains unclear. In the present study, we executed yeast two-hybrid screening using cDNA fragment of CLOCK PAS A domain as bait, and identified RANBP9 as a novel protein interacting with CLOCK. The interaction between CLOCK and RANBP9 was further validated by in vivo and in vitro assays. Previous studies have confirmed that SF3B3 was a RANBP9 interacting protein. Subsequently, our study also found that CLOCK and SF3B3 can interact with each other by co-immunoprecipitation in mouse testis...
November 7, 2017: Gene
https://www.readbyqxmd.com/read/29123028/the-cell-adhesion-molecule-bt-igsf-is-essential-for-a-functional-blood-testis-barrier-and-male-fertility-in-mice
#8
Laura Pelz, Bettina Purfürst, Fritz G Rathjen
The Ig-like cell adhesion molecule (IgCAM) brain- and testis-specific immunoglobulin superfamily (BT-IgSF) protein plays a major role in male fertility in mice. However, the molecular mechanism by which BT-IgSF supports fertility is unclear. Here, we found that it is localized in Sertoli cells at the blood-testis barrier (BTB) and at the apical ectoplasmic specialization. Absence of BT-IgSF in Sertoli cells in both global and conditional mouse mutants (i.e. AMHCre and Rosa26CreERT2 lines) resulted in male infertility, atrophic testes with vacuolation, azoospermia, and spermatogenesis arrest...
November 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29113556/phospholipase-d-family-member-6-pld6-is-a-surface-marker-for-enrichment-of-undifferentiated-spermatogonia-in-the-pre-pubertal-boars
#9
Pengfei Zhang, Yuwei Qin, Yi Zheng, Wenxian Zeng
Spermatogonial stem cells (SSCs) have a functional ability to maintain self-renewal and sustain production of spermatozoa throughout the reproductive lifespan of a male. Studies on SSCs can thus better understandings of spermatogenesis and unravel the mechanisms for self-renewal and differentiation of male germline stem cells. However, the rarity of SSCs in the testis and the lack of reliable surface markers obstruct the related study and further application of SSCs. This is especially the case in livestock animals...
November 7, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29100724/expression-of-the-gm2-activator-protein-in-mouse-testis
#10
Yu-Teh Li, Su-Chen Li, I-Li Chen
The GM2-activator protein (GM2-AP), revealed by Li et al. in 1973 in human liver, was initially identified as a protein cofactor that stimulated β-hexosaminidase A to hydrolyze N-acetylgalactosamine from GM2 ganglioside. This cofactor was found to be missing in human variant AB Tay-Sachs disease. Over the years, the GM2-AP has also been shown to be involved in kidney vesicular transport, lipid presentation by CD1 molecule to T-cells, and interaction of human sperm with zona pellucida. Since the expression of the GM2-AP via mRNA detection in mouse tissues was found to be the highest in testis, we became interested in the localization of the GM2-AP at cellular level in mouse testis during spermatogenesis...
October 31, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29098592/genotoxicity-of-carbon-tetrachloride-and-the-protective-role-of-essential-oil-of-salvia-officinalis-l-in-mice-using-chromosomal-aberration-micronuclei-formation-and-comet-assay
#11
Kawthar Ae Diab, Maha A Fahmy, Zeinab M Hassan, Emad M Hassan, Adel B Salama, Enayat A Omara
The present work was conducted to evaluate the genotoxic effect of carbon tetrachloride (CCl4) in mouse bone marrow and male germ cells. The safety and the modulating activity of sage (Salvia officinalis L.) essential oil (SEO) against the possible genotoxic effect of CCl4 were also evaluated. A combination of in vivo mutagenic endpoints was included: micronucleus (MN), apoptosis using dual acridine orange/ethidium bromide (AO/EB) staining, comet assay, chromosomal aberrations (CAs), and sperm abnormalities...
November 3, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29082348/expression-of-luteinizing-hormone-lh-subunit-genes-in-mouse-testis
#12
Hee Soo Kim, Sung-Ho Lee
Gonadotropins are heterodimers consisting an alpha chain (Cgα) and a beta chain. Interestingly, presence of complicated LH-β transcripts in rat testis was accidently found; testicular LH-β transcripts were confined in seminiferous tubules to spermatids, and the translated products were localized in the elongated spermatids. We hypothesized that mouse testis has potential to produce the tissue specific LH-β with similar structure to the rat testicular forms. To verify our hypothesis, we examined the adult mouse (ICR) testis using RT-PCR and immunohistochemistry...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29082235/tissue-and-cell-type-specific-expression-of-the-long-noncoding-rna-klhl14-as-in-mouse
#13
Sara Carmela Credendino, Nicole Lewin, Miriane de Oliveira, Swaraj Basu, Barbara D'Andrea, Elena Amendola, Luigi Di Guida, Antonio Nardone, Remo Sanges, Mario De Felice, Gabriella De Vita
lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described...
2017: International Journal of Genomics
https://www.readbyqxmd.com/read/29079676/sex-biased-microrna-expression-in-mammals-and-birds-reveals-underlying-regulatory-mechanisms-and-a-role-in-dosage-compensation
#14
Maria Warnefors, Katharina Mössinger, Jean Halbert, Tania Studer, John L VandeBerg, Isa Lindgren, Amir Fallahshahroudi, Per Jensen, Henrik Kaessmann
Sexual dimorphism depends on sex-biased gene expression, but the contributions of microRNAs (miRNAs) have not been globally assessed. We therefore produced an extensive small RNA sequencing data set to analyze male and female miRNA expression profiles in mouse, opossum, and chicken. Our analyses uncovered numerous cases of somatic sex-biased miRNA expression, with the largest proportion found in the mouse heart and liver. Sex-biased expression is explained by miRNA-specific regulation, including sex-biased chromatin accessibility at promoters, rather than piggybacking of intronic miRNAs on sex-biased protein-coding genes...
October 27, 2017: Genome Research
https://www.readbyqxmd.com/read/29072682/c-x-c-motif-chemokine-ligand-10-produced-by-mouse-sertoli-cells-in-response-to-mumps-virus-infection-induces-male-germ-cell-apoptosis
#15
Qian Jiang, Fei Wang, Lili Shi, Xiang Zhao, Maolei Gong, Weihua Liu, Chengyi Song, Qihan Li, Yongmei Chen, Han Wu, Daishu Han
Mumps virus (MuV) infection usually results in germ cell degeneration in the testis, which is an etiological factor for male infertility. However, the mechanisms by which MuV infection damages male germ cells remain unclear. The present study showed that C-X-C motif chemokine ligand 10 (CXCL10) is produced by mouse Sertoli cells in response to MuV infection, which induces germ cell apoptosis through the activation of caspase-3. CXC chemokine receptor 3 (CXCR3), a functional receptor of CXCL10, is constitutively expressed in male germ cells...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29069489/effects-of-intraperitoneal-injection-of-sns2-flowers-on-mouse-testicle
#16
Disi Bai, Qingzhao Li, Yanjie Xiong, Junjian Zhao, Liyuan Bai, Peijun Shen, Lu Yuan, Ping Wu
SnS 2 nanoflowers (SnS 2 NFs) have been widely used in photoelectric and catalytic applications. However, its explosure and reproductive toxicity is unknown. The aim of this study was to investigate the effect of exposure to three different sized-SnS 2 flowers (dose: 38 mg/Kg; size: 50, 80 and 200 nm) in testes of mice for 4 weeks by intraperitoneal injection. Though the body weight of mice treated or not with SnS 2 NFs was not different, and SnS 2 NFs were distributed to the organs including liver, kidney, spleen, heart, brain and testis, more distribution SnS 2 NFs (50 and 80 nm) were found in testicle tissues compared with SnS 2 flowers (200 nm) in those tissues...
October 23, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29060670/finite-element-modelling-and-preliminary-validation-of-microneedle-based-electrodes-for-enhanced-tissue-electroporation
#17
Ruth Houlihan, Konstantin Grygoryev, Zhenfei Ning, John Williams, Tom Moore, Conor O'Mahony
This paper investigates the use of microneedle-based electrodes for enhanced testis electroporation, with specific application to the production of transgenic mice. During the design phase, finite-element software has been used to construct a tissue model and to compare the relative performance of electrodes employing a) conventional flat plates, b) microneedle arrays, and c) invasive needles. Results indicate that microneedle-based electrodes can achieve internal tissue field strengths which are an order of magnitude higher than those generated using conventional flat electrodes, and which are comparable to fields produced using invasive needles...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29047395/cellular-and-molecular-characterization-of-gametogenic-progression-in-ex-vivo-cultured-prepuberal-mouse-testes
#18
J Isoler-Alcaraz, D Fernández-Pérez, E Larriba, J Del Mazo
BACKGROUND: Recently, an effective testis culture method using a gas-liquid interphase, capable of differentiate male germ cells from neonatal spermatogonia to spermatozoa has been developed. Nevertheless, this methodology needs deep analyses that allow future experimental approaches in basic, pathologic and/or reprotoxicologic studies. Because of this, we characterized at cellular and molecular levels the entire in vitro spermatogenic progression, in order to understand and evaluate the characteristics that define the spermatogenic process in ex vivo cultured testes compared to the in vivo development...
October 18, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29044429/dynamic-expression-of-long-noncoding-rnas-reveals-their-potential-roles-in-spermatogenesis-and-fertility
#19
Lauren Wichman, Saigopal Somasundaram, Christine Breindel, Dana M Valerio, John R McCarrey, Craig A Hodges, Ahmad M Khalil
Mammalian reproduction requires that males and females produce functional haploid germ cells through complex cellular differentiation processes known as spermatogenesis and oogenesis, respectively. While numerous studies have functionally characterized protein-coding genes and small noncoding RNAs (microRNAs and piRNAs) that are essential for gametogenesis, the roles of regulatory long noncoding RNAs (lncRNAs) are yet to be fully characterized. Previously, we and others have demonstrated that intergenic regions of the mammalian genome encode thousands of long noncoding RNAs, and many studies have now demonstrated their critical roles in key biological processes...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29042633/catsper1-promoter-is-bidirectional-and-regulates-the-expression-of-a-novel-lncrna
#20
Salma E Jiménez-Badillo, Norma Oviedo, Christian Hernández-Guzmán, Lorenza González-Mariscal, Javier Hernández-Sánchez
The Catsper1 gene, whose expression is restricted to male germ cells, has great importance in reproductive biology because of its function in sperm motility and fertilization. We previously reported that the promoter of this gene has transcriptional activity in either direction in a heterologous system. In the present study, we found that the Catsper1 promoter has in vitro transcriptional activity in either orientation in GC-1 spg mouse spermatogonial cells. The results also showed that this promoter regulates the expression of a new divergent Catsper1 gene named Catsper1au (Catsper1 antisense upstream transcript)...
October 17, 2017: Scientific Reports
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