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https://www.readbyqxmd.com/read/27902973/cd147-regulates-extrinsic-apoptosis-in-spermatocytes-by-modulating-nf%C3%AE%C2%BAb-signaling-pathways
#1
Chaoqun Wang, Kin Lam Fok, Zhiming Cai, Hao Chen, Hsiao Chang Chan
CD147 null mutant male mice are infertile with arrested spermatogenesis and increased apoptotic germ cells. Our previous studies have shown that CD147 prevents apoptosis in mouse spermatocytes but not spermatogonia. However, the underlying mechanism remains elusive. In the present study, we aim to determine the CD147-regulated apoptotic pathway in mouse spermatocytes. Our results showed that immunodepletion of CD147 triggered apoptosis through extrinsic apoptotic pathway in mouse testis and spermatocyte cell line (GC-2 cells), accompanied by activation of non-canonical NFκB signaling and suppression of canonical NFκB signaling...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27895724/expression-profile-of-spaca5-spaca5-in-spermatogenesis-and-transitional-cell-carcinoma-of-the-bladder
#2
Xiao-Yue Zhang, Qiu-Xia Yan, Xiao-Yan Guo, Cai-Rong Chen, Run-Qiang Chen, Zhi-Ming Cai, Ai-Fa Tang
The majority of bladder cancer-associated mortalities are due to transitional cell carcinoma (TCC), which is the most prevalent and chemoresistant malignancy of the bladder. Sperm acrosome associated 5 (SPACA5)/Spaca5 is a sperm acrosome-associated, c-type lysozyme-like protein that has been recently identified, and has been designated as an attractive candidate antigen for cancer testis. In the present study, the expression profile of SPACA5/Spaca5 was analyzed in spermatogenesis and TCC of the bladder using diverse molecular and cellular biology methods...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27884655/zika-virus-infection-in-dexamethasone-immunosuppressed-mice-demonstrating-disseminated-infection-with-multi-organ-involvement-including-orchitis-effectively-treated-by-recombinant-type-i-interferons
#3
Jasper Fuk-Woo Chan, Anna Jinxia Zhang, Chris Chung-Sing Chan, Cyril Chik-Yan Yip, Winger Wing-Nga Mak, Houshun Zhu, Vincent Kwok-Man Poon, Kah-Meng Tee, Zheng Zhu, Jian-Piao Cai, Jessica Oi-Ling Tsang, Kenn Ka-Heng Chik, Feifei Yin, Kwok-Hung Chan, Kin-Hang Kok, Dong-Yan Jin, Rex Kwok-Him Au-Yeung, Kwok-Yung Yuen
BACKGROUND: Disseminated or fatal Zika virus (ZIKV) infections were reported in immunosuppressed patients. Existing interferon-signaling/receptor-deficient mouse models may not be suitable for evaluating treatment effects of recombinant interferons. METHODS: We developed a novel mouse model for ZIKV infection by immunosuppressing BALB/c mice with dexamethasone. RESULTS: Dexamethasone-immunosuppressed male mice (6-8weeks) developed disseminated infection as evidenced by the detection of ZIKV-NS1 protein expression and high viral loads in multiple organs...
November 12, 2016: EBioMedicine
https://www.readbyqxmd.com/read/27871939/cell-cell-communication-mediated-by-the-car-subgroup-of-immunoglobulin-cell-adhesion-molecules-in-health-and-disease
#4
REVIEW
Claudia Matthäus, Hanna Langhorst, Laura Schütz, René Jüttner, Fritz G Rathjen
The immunoglobulin superfamily represents a diverse set of cell-cell contact proteins and includes well-studied members such as NCAM1, DSCAM, L1 or the contactins which are strongly expressed in the nervous system. In this review we put our focus on the biological function of a less understood subgroup of Ig-like proteins composed of CAR (coxsackievirus and adenovirus receptor), CLMP (CAR-like membrane protein) and BT-IgSF (brain and testis specific immunoglobulin superfamily). The CAR-related proteins are type I transmembrane proteins containing an N-terminal variable (V-type) and a membrane proximal constant (C2-type) Ig domain in their extracellular region which are implicated in homotypic adhesion...
November 18, 2016: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/27871845/inter-generational-effects-of-the-in-vitro-maturation-technique-on-pregnancy-outcomes-early-development-and-cognition-of-offspring-in-mouse-model
#5
N Wang, C E Ren, Y Y Lou, F Le, L Y Wang, X Z Liu, Q T Zhan, L N Mao, H Y Lou, F Jin
In vitro maturation (IVM) of oocytes has been a highly successful method for avoiding the occurrence of severe ovarian hyperstimulation syndrome in some patients during in vitro fertilization. However, the safety of the protocol, especially the long-term effects, is still an issue of debate. The current study is to investigate the long-term effects of IVM on mice through two generations and reveal its inter-generational effects as well. The data indicate that the rates of embryo resorption and fetal death in the F1 generation were significantly increased while the newborn survival rate in the F1 and F2 generations were significantly decreased in the IVM group...
November 18, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27869164/the-hot-spot-p53r172h-mutant-promotes-formation-of-giant-spermatogonia-triggered-by-dna-damage
#6
Y Xue, A Raharja, W Sim, E S M Wong, S A B Rahmat, D P Lane
Overexpression of mutant p53 is a common finding in most cancers but testicular tumours accumulate wild-type p53 (wtp53). In contrast to the accepted concept that p53 homozygous mutant mice do not accumulate mutant p53 in normal cells, our study on a mutant p53 mouse model of Li-Fraumeni syndrome harbouring the hot-spot p53R172H mutation described an elevated level of mutant p53 in non-cancerous mouse tissues. Here we use detailed immunohistochemical analysis to document the expression of p53R172H in mouse testis...
November 21, 2016: Oncogene
https://www.readbyqxmd.com/read/27856695/multiplex-shrna-screening-of-germ-cell-development-by-in-vivo-transfection-of-mouse-testis
#7
Nicholas R Y Ho, Abul R Usmani, Yan Yin, Liang Ma, Donald F Conrad
Spermatozoa are one of the few mammalian cells types that cannot be fully derived in vitro, severely limiting the application of modern genomic techniques to study germ cell biology. The current gold standard approach of characterizing single gene knockout mice is slow as generation of each mutant line can take 6-9 months. Here, we describe an in vivo approach to rapid functional screening of germline genes based on a new non-surgical, non-viral in vivo transfection method to deliver nucleic acids into testicular germ cells...
November 16, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27855777/successful-transmission-and-transcriptional-deployment-of-a-human-chromosome-via-mouse-male-meiosis
#8
Christina Ernst, Jeremy Pike, Sarah J Aitken, Hannah K Long, Nils Eling, Lovorka Stojic, Frances Connor, Tim F Rayner, Margus Lukk, Robert J Klose, Claudia Kutter, Duncan T Odom
Most human aneuploidies originate maternally, due in part to the presence of highly stringent checkpoints during male meiosis. Indeed, male sterility is common among aneuploid mice used to study chromosomal abnormalities, and male germline transmission of exogenous DNA has been rarely reported. Here we show that despite aberrant testis architecture, males of the aneuploid Tc1 mouse strain produce viable sperm and transmit human chromosome 21 to create aneuploid offspring. In these offspring, we mapped transcription, transcriptional initiation, enhancer activity, non-methylated DNA and transcription factor binding in adult tissues...
November 18, 2016: ELife
https://www.readbyqxmd.com/read/27852984/characterization-of-mageg2-with-testis-specific-expression-in-mice
#9
Juri Jeong, Sora Jin, Heejin Choi, Jun Tae Kwon, Jihye Kim, Jaehwan Kim, Zee Yong Park, Chunghee Cho
Male germ cell development is a well-defined process occurring in numerous seminiferous tubules of the testis. Uncovering testicular novel genes related to intrinsic regulation of spermatogenesis is essential for the understanding of spermatogenesis. In the present study, we investigated mouse Mageg2, which belongs to a group of melanoma-associated antigens (MAGEs). Mageg2 is transcribed in the testis specifically, and its expression level is increased at the pachytene spermatocyte stage, indicating that Mageg2 is expressed predominantly in germ cells...
November 15, 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27849015/sterile-inflammation-as-a-factor-in-human-male-infertility-involvement-of-toll-like-receptor-2-biglycan-and-peritubular-cells
#10
C Mayer, M Adam, L Glashauser, K Dietrich, J U Schwarzer, F-M Köhn, L Strauss, H Welter, M Poutanen, A Mayerhofer
Changes in the wall of seminiferous tubules in men with impaired spermatogenesis imply sterile inflammation of the testis. We tested the hypothesis that the cells forming the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), orchestrate inflammatory events and that Toll like receptors (TLRs) and danger signals from the extracellular matrix (ECM) of this wall are involved. In cultured HTPCs we detected TLRs, including TLR2. A TLR-2 ligand (PAM) augmented interleukin 6 (IL-6), monocyte chemo-attractant protein-1 (MCP-1) and pentraxin 3 (PTX3) in HTPCs...
November 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27835883/ranbpm-ranbp9-regulates-mouse-c-kit-receptor-level-and-is-essential-for-normal-development-of-bone-marrow-progenitor-cells
#11
Sandrine Puverel, Erkan Kiris, Satyendra Singh, Kimberly D Klarmann, Vincenzo Coppola, Jonathan R Keller, Lino Tessarollo
c-Kit is a tyrosine kinase receptor important for gametogenesis, hematopoiesis, melanogenesis and mast cell biology. Dysregulation of c-Kit function is oncogenic and its expression in the stem cell niche of a number of tissues has underlined its relevance for regenerative medicine and hematopoietic stem cell biology. Yet, very little is known about the mechanisms that control c-Kit protein levels. Here we show that the RanBPM/RanBP9 scaffold protein binds to c-Kit and is necessary for normal c-Kit protein expression in the mouse testis and subset lineages of the hematopoietic system...
November 8, 2016: Oncotarget
https://www.readbyqxmd.com/read/27826126/the-expression-patterns-of-tetratricopeptide-repeat-domain-36-ttc36
#12
Yuru Zhou, Qingling He, Jihui Chen, Yunhong Liu, Zhaomin Mao, Zhongshi Lyu, Dongsheng Ni, Yaoshui Long, Pan Ju, Jianing Liu, Yuping Gu, Qin Zhou
Tetratricopeptide repeat domain 36 (Ttc36), whose coding protein belongs to tetratricopeptide repeat (TPR) motif family, has not been studied extensively. We for the first time showed that Ttc36 is evolutionarily conserved across mammals by bioinformatics. Rabbit anti-mouse Ttc36 polyclonal antibody was generated by injecting synthetic full-length peptides through "antigen intersection" strategy. Subsequently, we characterized Ttc36 expression profile in mouse, showing its expression in liver and kidney both from embryonic day 15...
November 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27822884/lncrna-ak015322-promotes-proliferation-of-spermatogonial-stem-cell-c18-4-by-acting-as-a-decoy-for-microrna-19b-3p
#13
Ke Hu, Jing Zhang, Meng Liang
Long noncoding RNAs (lncRNAs) have been reported to play important roles in male reproduction. In our previous research, we studied the expression profile of lncRNAs in mouse male germ cells including spermatogonial stem cell, type A spermatogonia, pachytene spermatocyte, and round spermatid by microarray method, which showed that testis-enriched lncRNA AK015322 is highly expressed in spermatogonial stem cell. In this study, we found that AK015322 promotes proliferation of mouse spermatogonial stem cell line C18-4 in vitro...
November 7, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27818224/perfluorooctane-sulfonate-pfos-disrupts-blood-testis-barrier-by-down-regulating-junction-proteins-via-p38-mapk-atf2-mmp9-signaling-pathway
#14
Lianglin Qiu, Yingyun Qian, Zhenzhen Liu, Chao Wang, Jianhua Qu, Xiaoke Wang, Shoulin Wang
Perfluorooctane sulfonate (PFOS), an ubiquitous environmental pollutant, has been associated with male reproductive disorders. However, the underlying mechanisms are not yet fully understood. In this study, in vivo and in vitro models were used to explore the effects of PFOS on blood-testis barrier (BTB) and related molecular mechanisms. First, male ICR mice were orally administrated PFOS (0.5-10mg/kg/bw) for 4 weeks. Bodyweight, sperm count, BTB integrity and the expression of proteins including p38 mitogen-activated protein kinase (MAPK), activating transcription factor 2 (ATF2), matrix metalloproteinase 9 (MMP9), tissue inhibitor of metalloproteinase 1(TIMP1) and BTB related junction proteins were evaluated...
December 12, 2016: Toxicology
https://www.readbyqxmd.com/read/27815338/regulation-of-the-blood-testis-barrier-by-a-local-axis-in-the-testis-role-of-laminin-%C3%AE-2-in-the-basement-membrane
#15
Ying Gao, Dolores Mruk, Haiqi Chen, Wing-Yee Lui, Will M Lee, C Yan Cheng
Laminin α2 is one of the constituent components of the basement membrane (BM) in adult rat testes. Earlier studies that used a mouse genetic model have shown that a deletion of laminin α2 impedes male fertility by disrupting ectoplasmic specialization (ES; a testis-specific, actin-rich anchoring junction) function along the length of Sertoli cell in the testis. This includes ES at the Sertoli cell-elongating/elongated spermatid interface, which is known as apical ES and possibly the Sertoli-Sertoli cell interface, known as basal ES, at the blood-testis barrier (BTB)...
November 4, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27810530/fluoride-reduced-the-immune-privileged-function-of-mouse-sertoli-cells-via-the-regulation-of-fas-fasl-system
#16
Zilong Sun, Qingli Nie, Lianjie Zhang, Ruiyan Niu, Jundong Wang, Shaolin Wang
Previous investigations have demonstrated the adverse impacts of fluoride on Sertoli cells (SCs), such as oxidative stress and apoptosis. SCs are the crucial cellular components that can create the immune privileged environment in testis. However, the effect of fluoride on SCs immune privilege is unknown. In this study, mouse SCs were exposed to sodium fluoride with varying concentrations of 10(-5), 10(-4), and 10(-3) mol/L to establish the model of fluoride-treated SCs (F-SCs) in vitro. After 48 h of incubation, F-SCs were transplanted underneath the kidney capsule of mice for 21 days, or cocultured with spleen lymphocytes for another 48 h...
October 27, 2016: Chemosphere
https://www.readbyqxmd.com/read/27798603/zika-virus-infection-damages-the-testes-in-mice
#17
Jennifer Govero, Prabagaran Esakky, Suzanne M Scheaffer, Estefania Fernandez, Andrea Drury, Derek J Platt, Matthew J Gorman, Justin M Richner, Elizabeth A Caine, Vanessa Salazar, Kelle H Moley, Michael S Diamond
Zika virus (ZIKV) infection of pregnant women can cause congenital malformations including microcephaly, which has focused global attention on this emerging pathogen(1). In addition to transmission by mosquitoes, ZIKV can be detected in the seminal fluid of affected males for extended periods of time and transmitted sexually(2). Here, using a mouse-adapted African ZIKV strain (Dakar 41519), we evaluated the consequences of infection in the male reproductive tract of mice. We observed persistence of ZIKV, but not the closely related Dengue virus (DENV), in the testis and epididymis of male mice, and this was associated with tissue injury that caused diminished testosterone and inhibin B levels, and oligospermia...
October 31, 2016: Nature
https://www.readbyqxmd.com/read/27788151/sphingomyelin-synthase-1-is-essential-for-male-fertility-in-mice
#18
Anke Wittmann, Marcus O W Grimm, Harry Scherthan, Marion Horsch, Johannes Beckers, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabě de Angelis, Steven J Ford, Neal C Burton, Daniel Razansky, Dietrich Trümbach, Michaela Aichler, Axel Karl Walch, Julia Calzada-Wack, Frauke Neff, Wolfgang Wurst, Tobias Hartmann, Thomas Floss
Sphingolipids and the derived gangliosides have critical functions in spermatogenesis, thus mutations in genes involved in sphingolipid biogenesis are often associated with male infertility. We have generated a transgenic mouse line carrying an insertion in the sphingomyelin synthase gene Sms1, the enzyme which generates sphingomyelin species in the Golgi apparatus. We describe the spermatogenesis defect of Sms1-/- mice, which is characterized by sloughing of spermatocytes and spermatids, causing progressive infertility of male homozygotes...
2016: PloS One
https://www.readbyqxmd.com/read/27783995/mkp-1-attenuates-lps-induced-blood-testis-barrier-dysfunction-and-inflammatory-response-through-p38-and-i%C3%AE%C2%BAb%C3%AE-pathways
#19
Yiqing Pan, Yue Liu, Li Wang, Feng Xue, Yanqin Hu, Ran Hu, Chen Xu
Sertoli cells create a local tolerogenic microenvironment to maintain testicular immune privilege especially through the formation of a blood-testis barrier (BTB). However, the molecular mechanisms underlying the immune modulation function and BTB dynamics of Sertoli cells remained elusive. MAP phosphatase (MKP)-1 acts as a crucial negative regulator of the inflammatory response. Nevertheless, the role of MKP-1 in regulating Sertoli cells has not been elucidated. In this study, we have for the first time uncovered distinct cellular localization of MKP-1 in the cells at different stages of mouse testis, and the level of MKP-1 expression was significantly up-regulated by LPS-induced acute testis inflammation...
October 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27780883/human-umbilical-perivascular-cells-a-novel-source-of-mscs-to-support-testicular-niche-regeneration
#20
Leila Maghen, Ekaterina Shlush, Itai Gat, Melissa Filice, Tanya Barretto, Keith Jarvi, Kirk Lo, Andree S Gauthier-Fisher, Clifford L Librach
The expansion of functional testicular biopsy-derived human spermatogonial stem cells (hSSC) ex-vivo may enable the restoration of fertility in pre-pubertal males having undergone gonadotoxic therapies or men with severe male factor infertility. Various somatic cells are known to regulate SSC homeostasis and spermatogenesis in the developing and adult testis. Prior attempts to recapitulate this niche demonstrated the requirement of feeder cells, such as endogenous testicular somatic cells, for germ cell expansion ex-vivo...
October 25, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
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