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https://www.readbyqxmd.com/read/30409942/effectiveness-of-casein-phosphopeptide-amorphous-calcium-phosphate-and-xylitol-chewing-gums-on-salivary-ph-buffer-capacity-and-streptococcus-mutans-levels-an-interventional-study
#1
Krishnan Padminee, Saravanan Poorni, Davidson Diana, Dasarathan Duraivel, Manali Ramakrishnan Srinivasan
Aim: The aim of the study is to compare the anticariogenic effectiveness of Casein phosphopeptide- Amorphous Calcium phosphate (CPP-ACP) and xylitol chewing gums based on salivary pH, buffer capacity, and Streptococcus mutans levels. Materials and Methods: A group of twenty individuals in the age group of 18-25 years were randomly divided into two Groups A and B. Test arm A received xylitol gums and test arm B received CPP-ACP gums and they were instructed to use the gums thrice daily for 2 weeks...
September 2018: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
https://www.readbyqxmd.com/read/30409825/identification-of-active-site-residues-implies-a-two-step-catalytic-mechanism-for-acyl-acp-thioesterase
#2
Fuyuan Jing, Marna D Yandeau-Nelson, Basil J Nikolau
In plants and bacteria that use a Type II fatty acid synthase (FAS), isozymes of acyl-acyl carrier protein (ACP) thioesterase (TEs) hydrolyze the thioester bond of acyl-ACPs, terminating the process of fatty acid biosynthesis. These TEs are therefore critical in determining the fatty acid profiles produced by these organisms. Past characterizations of a limited number of plant-sourced acyl-ACP TEs have suggested a thiol-based, papain-like catalytic mechanism, involving a triad of Cys, His, and Asn residues...
November 8, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/30391532/screening-of-intestinal-probiotics-and-the-effects-of-feeding-probiotics-on-the-growth-immune-digestive-enzyme-activity-and-intestinal-flora-of-litopenaeus-vannamei
#3
Zhi-Han Zuo, Bi-Jiao Shang, Ying-Chun Shao, Wen-Yue Li, Jin-Sheng Sun
In this experiment, 426 strains were isolated from the intestinal tract of Litopenaeus vannamei, and 11 strains showed strong digestive enzyme production activity and antagonistic effect against common bacterial pathogens of shrimp. After hemolysis activity test and drug sensitivity test, 2 candidate probiotics with good bacteriostatic activity, strong enzyme production ability and relatively sensitive to antibiotics were screened out, and were identified by 16s rDNA molecular identification and Biolog-System as Enterobacter hominis (E3) and lactobacillus (L3)...
November 1, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30388050/breaking-barriers-prospective-study-of-a-cohort-of-advanced-chronic-obstructive-pulmonary-disease-patients-to-describe-their-survival-and-end-of-life-palliative-care-requirements
#4
Daniel Gainza-Miranda, Eva Maria Sanz-Peces, Alberto Alonso-Babarro, María Varela-Cerdeira, Concepción Prados-Sánchez, Guadalupe Vega-Aleman, Ricardo Rodriguez-Barrientos, Elena Polentinos-Castro
BACKGROUND AND AIM: Consensus has been reached on the need to integrate palliative care in the follow-up examinations of chronic obstructive pulmonary disease (COPD) patients. We analyzed the survival from the initiation of follow-up by a palliative home care team (PHCT) and described the needs and end-of-life process. SETTING AND DESIGN: This study was a prospective observational cohort study of advanced COPD patients referred to a PHCT. Sociodemographic variables, survival from the start date of follow-up using the Kaplan-Meier model, health resource consumption, perceived quality of life, main symptomatology, opioid use, and advanced care planning (ACP) were analyzed...
November 2, 2018: Journal of Palliative Medicine
https://www.readbyqxmd.com/read/30386500/the-effect-of-diode-laser-irradiation-associated-with-photoabsorbing-agents-containing-remineralizing-materials-on-microhardness-morphology-and-chemical-structure-of-early-enamel-caries
#5
Farzaneh Ahrari, Hamideh-Sadat Mohammadipour, Ladan Hajimomenian, Amir Fallah-Rastegar
Background: This study investigated the effects of laser irradiation associated with photo-absorbing agents containing sodium fluoride (NaF), MI paste Plus or Remin Pro® on microhardness and surface structure of white spot lesions (WSLs). Material and Methods: Fifty-six premolars were divided into two halves, then immersed in a demineraling solution to induce WSLs. The samples were divided into 8 groups by treatment (n=12) : (1) control, (2) diode laser (810 nm, 500 mW, 90 s), (3) NaF, (4) MI Paste plus, (5) Remin Pro®, (6) NaF + Laser, (7) MI Paste Plus + Laser, (8) Remin Pro® + Laser...
October 2018: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/30383132/reducing-firearm-injuries-and-deaths-in-the-united-states-a-position-paper-from-the-american-college-of-physicians
#6
Renee Butkus, Robert Doherty, Sue S Bornstein
For more than 20 years, the American College of Physicians (ACP) has advocated for the need to address firearm-related injuries and deaths in the United States. Yet, firearm violence continues to be a public health crisis that requires the nation's immediate attention. The policy recommendations in this paper build on, strengthen, and expand current ACP policies approved by the Board of Regents in April 2014, based on analysis of approaches that the evidence suggests will be effective in reducing deaths and injuries from firearm-related violence...
October 30, 2018: Annals of Internal Medicine
https://www.readbyqxmd.com/read/30375899/dilemmas-adult-children-face-in-discussing-end-of-life-care-preferences-with-their-parents
#7
Lori A Roscoe, Philip Barrison
This study explored the perceived goals, barriers, and strategies that characterize family interactions about advance care planning (ACP), which is instrumental in guiding end-of-life care. Discussions within the family context can significantly improve end-of-life decision making but are complicated, partly because participants are attempting to achieve multiple, and often competing, goals. Participants (n = 75) responded to a hypothetical scenario about a conversation with a parent about ACP by completing an anonymous online survey...
October 30, 2018: Health Communication
https://www.readbyqxmd.com/read/30375646/the-potential-ecological-effects-of-casein-phosphopeptide-amorphous-calcium-phosphate-in-dental-caries-prevention
#8
REVIEW
Nebu Philip, Laurence Walsh
Contemporary caries prevention protocols recommend not only effective remineralizing agents but also ecological measures to reverse the dental plaque dysbiosis responsible for the disease pathogenesis. There is a high-level of evidence supporting the remineralizing efficacy of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) from studies around the world. There is now emerging evidence that CPP-ACP may also have a beneficial influence on the dental plaque microbial ecology and homeostasis. The ecological cariostatic effects of CPP-ACP are believed to be mediated predominantly through its anti-adhesion, buffering, and biofilm disrupting actions...
October 30, 2018: Australian Dental Journal
https://www.readbyqxmd.com/read/30375014/the-role-of-mentorship-in-trainee-advanced-clinical-practitioner-development
#9
EDITORIAL
Nina O'Grady
Advanced Clinical Practitioners (ACPs) are a rapidly growing workforce worldwide with the USA showing the highest absolute number and rate per population, followed by the Netherlands, Canada, Australia, Ireland and New Zealand (Maier et al 2016). Whilst many practitioners are nurses by background, the development of the role has encouraged the inclusion of other professions such as physiotherapists, pharmacists and paramedics. The progression of the ACP role involves the acquisition of new skills alongside post graduate academic education at Masters level and peer support (NHS Education for Scotland 2012, Aguilard et al 2017, Health Education England 2017)...
October 30, 2018: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/30370280/physiology-guided-management-of-hemodynamics-in-acute-respiratory-distress-syndrome
#10
REVIEW
Gustavo A Cortes-Puentes, Richard A Oeckler, John J Marini
Skillfully implemented mechanical ventilation (MV) may prove of immense benefit in restoring physiologic homeostasis. However, since hemodynamic instability is a primary factor influencing mortality in acute respiratory distress syndrome (ARDS), clinicians should be vigilant regarding the potentially deleterious effects of MV on right ventricular (RV) function and pulmonary vascular mechanics (PVM). During both spontaneous and positive pressure MV (PPMV), tidal changes in pleural pressure (PPL ), transpulmonary pressure (PTP , the difference between alveolar pressure and PPL ), and lung volume influence key components of hemodynamics: preload, afterload, heart rate, and myocardial contractility...
September 2018: Annals of Translational Medicine
https://www.readbyqxmd.com/read/30369600/a-randomised-controlled-trial-of-an-advance-care-planning-intervention-for-patients-with-incurable-cancer
#11
Stephanie B Johnson, Phyllis N Butow, Melanie L Bell, Karen Detering, Josephine M Clayton, William Silvester, Belinda E Kiely, Stephen Clarke, Lisa Vaccaro, Martin R Stockler, Phillip Beale, Natalie Fitzgerald, Martin H N Tattersall
BACKGROUND: We modified and evaluated an advance care planning (ACP) intervention, which had been shown to improve compliance with patient's end of life (EoL) wishes, in a different patient population. METHODS: Patients with incurable cancer, and a Family Member (FM), were randomised one-to-one to usual care or usual care plus an ACP intervention, between April 2014 and January 2017. Oncologists and participants were non-blinded. ACP was based on the Respecting Patient Choices model, with an offer to provide individualised ranges for typical, best-case and worst-case scenarios for survival time...
October 29, 2018: British Journal of Cancer
https://www.readbyqxmd.com/read/30367747/uptake-of-soil-applied-thiamethoxam-in-orange-and-its-effect-against-asian-citrus-psyllid-in-different-seasons
#12
Wei Hu, Fan Kuang, Jiong Chun, Zhanjun Lu, Xingtao Li, Qiyang Zhao, Balian Zhong, Huanan Su, Zhixiang Zhang, Ning Zhang
BACKGROUND: Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is an important pest of citrus worldwide because it transmits the causal bacteria causing huanglongbing (HLB). We investigated the effects and persistence of two soil application rates of thiamethoxam on ACP populations in two flushing seasons in the field. The residues of thiamethoxam and clothianidin in the fruit were detected to evaluate food safety. RESULTS: Soil application of 50% thiamethoxam water dispersible granule at concentrations of 4 and 10 g tree-1 significantly decreased ACP populations, and there was a positive correlation between the control efficacy and persistence of thiamethoxam and clothianidin in leaves, providing longer-term protection up to 90 days in the fall compared with 60 days in the spring...
October 27, 2018: Pest Management Science
https://www.readbyqxmd.com/read/30366785/bioassay-guided-fractionation-of-cyclea-peltata-using-in-vitro-raw-264-7-cell-culture-antioxidant-assays-and-isolation-of-bioactive-compound-tetrandrine
#13
V J Shine, G I Anuja, S R Suja, Gopan Raj, P G Latha
BACKGROUND: Cyclea peltata is one of the herbs mentioned in ancient scriptures of Ayurveda and is used in different types of Ayurvedic gritham preparations. Moreover, in traditional/tribal medicine C. peltata is used as digestive, anti-inflammatory, diuretic and to treat jaundice, digestive disorders, etc. OBJECTIVE: Activity guided fractionation of C. peltata and in correlation with the levels of bioactive compound tetrandrine. MATERIALS AND METHODS: Preliminary phytochemical screening, estimation of total alkaloid content, preparation of different extracts of C...
October 23, 2018: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/30364715/-in-situ-raman-spectroscopy-of-amorphous-calcium-phosphate-to-crystalline-hydroxyapatite-transformation
#14
Jessica A Stammeier, Bettina Purgstaller, Dorothee Hippler, Vasileios Mavromatis, Martin Dietzel
Amorphous calcium phosphate (Ca3 (PO4 )2 xnH2 O; n = 3-4.5; ACP) is a precursor phase of the mineral hydroxyapatite (Ca5 (PO4 )3 (OH); HAP) that in natural settings occurs during both authigenic and biogenic mineral formation. In aqueous solutions ACP transforms rapidly to the crystalline phase. The transformation rate is highly dependent on the prevailing physico-chemical conditions, most likely on: Ca & PO4 concentration, pH and temperature. In this study, we conducted a calcium phosphate precipitation experiment at 20 °C and pH 9...
2018: MethodsX
https://www.readbyqxmd.com/read/30359752/dietary-yeast-culture-modulates-immune-response-related-to-tlr2-myd88-nf-k%C3%AE-signaling-pathway-antioxidant-capability-and-disease-resistance-against-aeromonas-hydrophila-for-ussuri-catfish-pseudobagrus-ussuriensis
#15
Xianyong Bu, Xuqiu Lian, Yi Wang, Chengzeng Luo, Shengqiang Tao, Yilu Liao, Jiaming Yang, Aijing Chen, Yuhong Yang
The aim of the present study was to investigate effects of dietary yeast culture on immune response related to TLR2-MyD88-NF-kβ signaling pathway, antioxidant capability and disease resistance against Aeromonas hydrophila for Ussuri catfish (Pseudobagrus ussuriensis). A total of 240 Ussuri catfish (mean weight of 7.39 ± 0.32 g) were randomly distributed into four groups that fed diets containing 0 (Y0), 10 (Y1), 20 (Y2) and 30 (Y3) g kg-1 yeast culture for 8 weeks. The results indicated that dietary 10 g kg-1 yeast culture supplementation significantly down-regulated mRNA levels of TLR2, MyD88, NF-kβ p65, IL-1β and IL-8 in the liver tissue compared with the control group (P < 0...
October 22, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30356983/contribution-of-mitophagy-to-cell-mediated-mineralization-revisiting-a-50-year-old-conundrum
#16
Dan-Dan Pei, Jin-Long Sun, Chun-Hui Zhu, Fu-Cong Tian, Kai Jiao, Matthew R Anderson, Cynthia Yiu, Cui Huang, Chang-Xiong Jin, Brian E Bergeron, Ji-Hua Chen, Franklin R Tay, Li-Na Niu
Biomineralization in vertebrates is initiated via amorphous calcium phosphate (ACP) precursors. These precursors infiltrate the extracellular collagen matrix where they undergo phase transformation into intrafibrillar carbonated apatite. Although it is well established that ACP precursors are released from intracellular vesicles through exocytosis, an unsolved enigma in this cell-mediated mineralization process is how ACP precursors, initially produced in the mitochondria, are translocated to the intracellular vesicles...
October 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/30354045/de-novo-design-and-implementation-of-a-tandem-acyl-carrier-protein-domain-in-a-type-i-modular-polyketide-synthase
#17
Zilong Wang, Saket R Bagde, Gerardo Zavala, Tsutomu Matsui, Xi Chen, Chu-Young Kim
During polyketide and fatty acid biosynthesis, the growing acyl chain is attached to the acyl carrier protein via a thioester linkage. The acyl carrier protein interacts with other enzymes that perform chain elongation and chain modification on the bound acyl chain. Most type I polyketide synthases and fatty acid synthases contain only one acyl carrier protein. However, polyunsaturated fatty acid synthases from deep-sea bacteria contain anywhere from two to nine acyl carrier proteins. Recent studies have shown that this tandem acyl carrier protein feature is responsible for the unusually high fatty acid production rate of deep-sea bacteria...
October 24, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/30353570/cytochemical-characterization-of-peripheral-blood-cell-populations-of-two-cyprinidae-carassius-auratus-and-ctenopharyngodon-idellus
#18
Fumiao Zhang, Ranran Feng, Wei Fang, Yanhui Shi, Liguo An, Guiwen Yang
Fish are the most diverse species of all vertebrate groups, and their blood cells have shown variable characteristics in terms of morphology. Cytochemical staining for enzyme activity in blood leukocytes will help assess the immune function of fish. We characterize blood cells from crucian carp (Carassius auratus) and grass carp (Ctenopharyngodon idellus) by using a Diff-Quick stain as well as different cytochemical methods. Blood specimens obtained from crucian carp and grass carp were evaluated after cytochemical staining for acid phosphatase (ACP), alkaline phosphatase (ALP), naphthol AS chloroacetate esterase (AS-DNCE), naphthyl acetate esterase (NAE), α-naphthyl butyrate esterase (NBE), peroxidase (MPO) and periodic acid-Schiff's reaction (PAS) using commercial kits...
October 23, 2018: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/30352934/identification-of-novel-protein-lysine-acetyltransferases-in-escherichia-coli
#19
David G Christensen, Jesse G Meyer, Jackson T Baumgartner, Alexandria K D'Souza, William C Nelson, Samuel H Payne, Misty L Kuhn, Birgit Schilling, Alan J Wolfe
Posttranslational modifications, such as N ε-lysine acetylation, regulate protein function. N ε-lysine acetylation can occur either nonenzymatically or enzymatically. The nonenzymatic mechanism uses acetyl phosphate (AcP) or acetyl coenzyme A (AcCoA) as acetyl donor to modify an N ε-lysine residue of a protein. The enzymatic mechanism uses N ε-lysine acetyltransferases (KATs) to specifically transfer an acetyl group from AcCoA to N ε-lysine residues on proteins. To date, only one KAT (YfiQ, also known as Pka and PatZ) has been identified in Escherichia coli Here, we demonstrate the existence of 4 additional E...
October 23, 2018: MBio
https://www.readbyqxmd.com/read/30348430/a-novel-fluorescence-turn-off-on-nanosensor-for-sensitivity-detection-acid-phosphatase-and-inhibitor-based-on-glutathione-functionalized-graphene-quantum-dots
#20
Zhengyi Qu, Ning Li, Weidan Na, Xingguang Su
In this paper, we developed a label-free and sensitive fluorescence sensor for acid phosphatase (ACP) and its inhibitor parathion-methyl (PM) detection based on glutathione-functionalized graphene quantum dots (GQDs@GSH). Upon addition of MnO2 nanosheets, the fluorescence of GQDs@GSH could be efficiently quenched via a fluorescence resonance energy transfer. ACP could easily catalyze the hydrolysis of L-Ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), which could reduce MnO2 nanosheets to Mn2+ in acidic environment, leading to dramatically increase of the fluorescence intensity of GQDs@GSH...
January 15, 2019: Talanta
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