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Yueqi An, Qing Liu, Yaru Xie, Shanbai Xiong, Tao Yin, Ru Liu
BACKGROUND: Ultrasound and Ca2+ have been used separately to increase myosin properties in fish processing. However, little literature was found about myosin changes affected by Ca2+ and ultrasound in combination. The goal of this study was to investigate the effects of ultrasound-calcium combination system on aggregation and conformational changes of silver carp myosin. RESULTS: Ultrasonic facilitated the Ca2+ -induced increase of turbidity. As Ca2+ concentration increased from 0 to 100 mmol L-1 , there was an obvious increase in the turbidity, solubility and mean hydrated particle size of myosin after ultrasonic treatment compared to those without ultrasonic...
April 15, 2018: Journal of the Science of Food and Agriculture
Yaoqi Wang, Xing Wang, Feiyang Deng, Nan Zheng, Yanqin Liang, Hua Zhang, Bing He, Wenbing Dai, Xueqing Wang, Qiang Zhang
Drug-drug conjugate nanoparticles (DDC NPs) is a potential method for overcoming poor solubility and nonspecific action in cancer therapy, which is based on its high drug loading efficiency and passive tumor-target properties. Our laboratory has prepared DOX-SS-DOX NPs based on disulfide-linked doxorubicin (DOX) drug-drug conjugate, which showed well physical stability and similar anti-tumor efficacy as liposomes. However, how structures of DDCs influence the self-assembling and anti-tumor efficacy is still seldom clarified and needs further investigation...
April 12, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
A Sh Sadulaeva, K I Lysenko, I D Stulin, A I Panevin
AIM: To determine predictors of remote outcomes of complex combat brain injury. MATERIAL AND METHODS: A survey of 145 combatants with long-term combat injury (from 1 to 10 years) was conducted. Responses were received from 30 people from 18 regions of the Russian Federation. Data analysis included nonparametric correlation analysis using Spearman coefficient. RESULTS AND CONCLUSION: Significant correlations were between the remote functional outcome and the following predictors: the severity of injury at admission (r=0...
2018: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
Do Yeon Lee, Seung Hyun Hong, Bokyung Kim, Dong-Seok Lee, Kweon Yu, Kyu-Sun Lee
The unfolded protein response (UPR) is an evolutionarily conserved adaptive reaction that increases cell survival under endoplasmic reticulum (ER) stress conditions. ER stress-associated neuronal cell death pathways play roles in the pathogenesis of neurodegenerative diseases, including Alzheimer's, Parkinson's, and Huntington's disease. Neuropeptide Y (NPY) has an important role in neuroprotection against neurodegenerative diseases. In this study, we investigated whether NPY has a protective role in ER stress-induced neuronal cell death in SK-N-SH human neuroblastoma cells...
April 6, 2018: European Journal of Cell Biology
Marcos Lahuerta, Carmen Aguado, Pablo Sánchez-Martín, Pascual Sanz, Erwin Knecht
Lafora disease (LD) is a fatal neurodegenerative disorder caused mostly by mutations in either of two genes encoding laforin and malin. LD is characterized by accumulation of a poorly-branched form of glycogen in the cytoplasm of neurons and other cells. We previously reported dysfunctional mitochondria in different LD models. Now, using mitochondrial uncouplers and respiratory chain inhibitors, we have investigated with human fibroblasts a possible alteration in the selective degradation of damaged mitochondria (mitophagy) in LD...
April 12, 2018: FEBS Journal
Syed Saad Hussain, Kinza Rafi, Shaheen Faizi, Zaid Abdul Razzak, Shabana U Simjee
Neuroblastoma being the most common extracranial pediatric solid tumor accounts for 15% of overall cancer-related childhood mortalities. Resistance to chemotherapeutic drugs is one of the limiting factors for positive prognosis for neuroblastoma. Therefore, there is always a need for developing new therapeutic moieties which can become a future prospect of neuroblastoma therapy. Terpenoids being the largest natural compounds have demonstrated many biological activities including anticancer activity. Keeping in mind the role of terpenoids in biological system, we aimed to identify novel semi-synthetic terpenoid derived from cleroda diterpene, 16-oxo-cleroda-3,13(14)E-diene-15-oic acid (1) as a potential anticancer moiety against neuroblastoma...
April 11, 2018: Molecular and Cellular Biochemistry
Hendrik Walther, Chau-Minh Phan, Lakshman N Subbaraman, Lyndon Jones
Purpose: We evaluate the differences in lipid uptake and penetration in daily disposable (DD) contact lenses (CL) using a conventional "in-vial" method compared to a novel in vitro eye model. Methods: The penetration of fluorescently labelled 22-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-23,24-Bisnor-5-Cholen-3beta-Ol (NBD)-cholesterol on three silicone hydrogel (SH) and four conventional hydrogel (CH) DD CLs were investigated. CLs were incubated for 4 and 12 hours in a vial, containing 3...
April 2018: Translational Vision Science & Technology
Bo Tang, Da-Wei He, Dian Li, Wen-Hao Guo, Dan Zhang, Guang-Hui Wei
OBJECTIVE: To investigate the effect of outer membrane vesicles derived from Escherichia coli on proliferation, apoptosis and migration of human neuroblastoma SK-N-SH cells in vitro. METHODS: The outer membrane vesicles (OMVs) were obtained from wild-type Escherichia coli with ultracentrifugation method, and the morphology of the OMVs was observed by transmission electron microscopy and the vesicle diameter was determined using MALVERN ZEN3690. Human neuroblastoma SK-N-SH cells were treated with the OMVs at low (100 µg/mL), moderate (500 µg/mL) and high (1000 µg/mL) doses, and 24, 48 and 72 h later, the cell proliferation activity was detected with MTT assay...
March 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Yue-Qin Zeng, Yin-Bo Cui, Juan-Hua Gu, Chen Liang, Xin-Fu Zhou
Alzheimer's disease (AD) is pathologically characterized by excessive accumulation of amyloid-beta (Aβ) within extracellular spaces of the brain. Aggregation of Aβ has been shown to trigger oxidative stress, inflammation, and neurotoxicity resulting in cognitive dysfunction. In this study, we use models of cerebral Aβ amyloidosis to investigate anti-amyloidogenic effects of scutellarin in vitro and in vivo. Our results show that scutellarin, through binding to Aβ42, efficiently inhibits oligomerization as well as fibril formation and reduces Aβ oligomer-induced neuronal toxicity in cell line SH-SY5Y...
April 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Diego Rocha-Parra, Jorge Chirife, Clara Zamora, Sonia de Pascual-Teresa
Red wine polyphenols are known for their implications for human health protection, although they suffer from high instability. For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF). More than thirty polyphenols, including anthocyanins, flavanols, flavonols, phenolic acids and stilbenoids, were identified...
April 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Alec Miners, Carrie Llewellyn, Carina King, Alex Pollard, Anupama Roy, Richard Gilson, Alison Rodger, Fiona Burns, Maryam Shahmanesh
To understand whether people attending sexual health (SH) clinics are willing to participate in a brief behavioural change intervention (BBCI) to reduce the likelihood of future sexually transmitted infections (STIs) and to understand their preferences for different service designs, we conducted a discrete choice experiment (DCE) with young heterosexual adults (aged 16-25 years), and men who have sex with men (MSM) aged 16 or above, attending SH clinics in England. Data from 368 participants showed that people particularly valued BBCIs that involved talking (OR 1...
January 1, 2018: International Journal of STD & AIDS
Ah Young Lee, Ji Myung Choi, Myoung Hee Lee, Jaemin Lee, Sanghyun Lee, Eun Ju Cho
BACKGROUND/OBJECTIVE: Oxidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. The aim of present study was to investigate the neuroprotective effects of perilla oil (PO) and its active component, alpha-linolenic acid (ALA), against hydrogen peroxide (H2 O2 )-induced oxidative stress in SH-SY5Y neuronal cells. MATERIALS/METHODS: The SH-SY5Y human neuroblastoma cells exposed to 250 µM H2 O2 for 24 h were treated with different concentrations of PO (25, 125, 250 and 500 µg/mL) and its major fatty acid, ALA (1, 2...
April 2018: Nutrition Research and Practice
Yafang Chen, Junhui Sui, Qing Wang, Yijia Yin, Jun Liu, Qiguang Wang, Xianglong Han, Yong Sun, Yujiang Fan, Xingdong Zhang
The injectable self-crosslinking blend hydrogel by combination of collagen I and thiolated hyaluronic acid could alleviate collagen I contraction in vitro and overcome weak cell adhesive sites of hyaluronic acid. Five groups of injectable hydrogels with different ratios were prepared to investigate their gelation time, injection force, mechanical properties, swelling capacity and disintegration performance. These results indicated that Col7 HA-SH3 hydrogel achieved the optimal controlled and injectable effect, the gelation time was just ten seconds with injection force at 3...
June 15, 2018: Carbohydrate Polymers
Jinghan Feng, Xingmiao Chen, Shengwen Lu, Wenting Li, Dan Yang, Weiwei Su, Xijun Wang, Jiangang Shen
Excessive autophagy/mitophagy plays important roles during cerebral ischemia-reperfusion (I/R) injury. Peroxynitrite (ONOO- ), a representative reactive nitrogen species, mediates excessive mitophagy activation and exacerbates cerebral I/R injury. In the present study, we tested the hypothesis that naringin, a natural antioxidant, could inhibit ONOO- -mediated mitophagy activation and attenuate cerebral I/R injury. Firstly, we demonstrated that naringin possessed strong ONOO- scavenging capability and also inhibited the production of superoxide and nitric oxide in SH-SY5Y cells exposed to 10 h oxygen-glucose-deprivation plus 14 h of reoxygenation or ONOO- donor 3-morpholinosydnonimine conditions...
April 7, 2018: Molecular Neurobiology
Carolin Stöckl, Rüdiger Hampe, Bogna Stawarczyk, Miriam Haerst, Malgorzata Roos
STATEMENT OF PROBLEM: The selection of an appropriate restorative material based on wear behavior is important for the long-term success of a dental restoration. For computer-aided design and computer-aided manufacturing (CAD-CAM) composite resins, information about their wear resistance and wear mechanism is scarce. PURPOSE: The purpose of this in vitro study was to compare the 2- and 3-body wear of CAD-CAM composite resins with that of lithium disilicate ceramic and to develop analysis software...
April 5, 2018: Journal of Prosthetic Dentistry
Peng Cai, Hongxing Li, Weiwei Huo, Hairong Zhu, Chao Xu, Rujin Zang, Wei Lv, Yankai Xia, Weibing Tang
Hirschsprung's disease (HSCR) is a birth defect that causes a failure of the enteric nervous system to cover the distal gut during early embryonic development. Evidence shows that long non-coding RNAs (lncRNA) play important roles in HSCR. The MIR31 host gene (MIR31HG), also known as Loc554202, is a long non-coding RNA (lncRNA), which acts as the host gene of (microRNA) miR-31 and miR-31*. There have been no studies regarding its function in early developmental defects during pregnancy, and its downstream genetic receptors...
April 6, 2018: Journal of Cellular Biochemistry
Yanchao Cui, Renchun Huang, Yingzhou Wang, Li Zhu, Xueliang Zhang
OBJECTIVE: The Leucine-rich repeat-containing G-protein coupled receptor 6 (LGR6) is a well-known marker of stem cells. In present study, we aimed to further explore the effects of LGR6 on promoting osteogenic differentiation of bone marrow stromal cells (BMSCs) and bone healing. METHODS: Flow cytometry assay was used to determine the expression of BMSCs surface markers, and western blot was performed to detect the LGR6 protein expression. The osteogenic differentiation of BMSCs was qualified using ALP and ARS staining...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Natalya Sh Lebedeva, Elena S Yurina, Yury A Gubarev, Sergey A Syrbu
The interaction of tetracationic porphyrins with DNA was studied using UV-Vis absorption, fluorescence spectroscopy and viscometry, and the particle sizes were determined. Аs cationic porphyrins, two isomer porphyrins, 3,3',3″,3‴-(5,10,15,20-Porphyrintetrayl)tetrakis(1-methylpyridinium) (TMPyP3) and 4,4',4″,4‴-(5,10,15,20-Porphyrintetrayl)tetrakis(1-methylpyridinium) (TMPyP4), were studied. They differ in the position of NCH3 + group in phenyl ring of the porphyrins and hence, in degree of freedom of rotation of the phenyl rings about the central macrocycle...
March 26, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Natalya F Naumenko
Multilayered substrates, including thin plates of LiTaO3 (LT) bonded to silicon (Si) or glass wafers either directly or via intermediate layers, were numerically investigated as potential materials for surface acoustic wave (SAW) resonators with high quality (Q)-factor required for the next generation of mobile communication systems. The propagation velocities and electromechanical coupling coefficients of shear horizontally (SH) polarized acoustic waves were estimated as functions of LT orientation and thicknesses of all layers in LT/Si, LT/SiO2 /Si, LT/glass, LT/AlN/glass and structures with several pairs of SiO2 /AlN layers between LT and the glass wafer...
March 31, 2018: Ultrasonics
Basma A Omran, Hussein N Nassar, Nesreen A Fatthallah, A Hamdy, Einas H El-Shatoury, Nour Sh El-Gendy
AIMS: Since mycosynthesis of metal nanoparticles is advertised as a promising and eco- friendly approach. Thus, the present study aims to investigate the capability of Aspergillus brasiliensis ATCC 16404 for mycosynthesis of silver nanoparticles (AgNPs). METHODS AND RESULTS: One-factor-at-a-time-technique was used to study the effect of different physicochemical parameters; the reaction time, pH, temperature, different stirring rates, illumination and finally the different concentrations of silver nitrate and fungal biomass on the mycosynthesis of AgNPs...
April 6, 2018: Journal of Applied Microbiology
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