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Nguyễn Hoàng Ly, Thanh Danh Nguyen, Sang-Woo Joo
Zidovudine (AZT) adsorbed on colloidal gold nanoparticles (AuNPs) undergoes pH-induced conformational changes according to spectral changes in surface-enhanced Raman scattering (SERS). In acidic pH values conditions, AZT assumes the C(2') -endo conformer, which binds more weakly to AuNPs than under neutral and alkaline conditions. In this study, density functional theory (DFT) calculations were performed; these calculations also supported the conformation-dependent binding energies. A lactobionic acid-conjugated PEGylated (LA-PEG-SH; molecular weight: 3400) unit was attached to AuNPs to target the asialoglycoprotein receptors overexpressed in hepatocarcinoma cells of Huh7 and SNU-354...
May 19, 2018: Colloids and Surfaces. B, Biointerfaces
Haigang Liu, Xiaohui Zhao, Hui Li, Yuanlin Zheng, Xianfeng Chen
The nonlinear holography technique is a powerful tool for all-optical switching and manipulation of an arbitrary harmonic wave. The common method of realizing such nonlinear holography is by configuring the structure of nonlinear photonic crystals. However, it is a challenge to dynamically tune the harmonic wave pattern. To overcome the long-term existing non-dynamic property of such nonlinear holographs, we realize dynamic computer-generated nonlinear optical holograms in the non-collinear second-harmonic (SH) generation process in which only one infrared beam is modulated...
July 15, 2018: Optics Letters
Lu Yao, Xing Dai, Yina Sun, Yong Wang, Qian Yang, Xinlin Chen, Yong Liu, Li Zhang, Wen Xie, Jian Liu
Monoamine oxidase B (MAO B) inhibitors, which inhibit dopamine decomposition by antagonizing MAO B activity, are approved and widely used for clinical treatment of Parkinson's disease (PD). Nonetheless, the mechanism of the abnormally increased MAO B activity in PD is still unclear. Previous research showed transcription factor specificity protein 1 (SP1) directly regulates MAO B activity by binding the SP1 binding sequence in MAO B promoter. In our study, we first observed that the SP1 protein level and SP1 binding activity in the MAO B promoter were increased in 1-methyl-4-phenylpyridinium (MPP+ ) neurotoxin-induced SH-SY5Y cells...
July 13, 2018: Journal of Neuroscience Research
Atif Abdulwahab A Oyouni, Shalini Saggu, Ehab Tousson, Hasibur Rehman
Tacrolimus (TAC) is used sporadically as an immunosuppressive agent for organ transplantation, but its clinical used is limited due to its marked nephrotoxicity. Ocimum basilicum L. (Lamiaceae) (OB) had been shown to possess antioxidant, anti-inflammatory and nephroprotective activity, and effective at improving renal inflammation and glomerular. In our study, we aim to evaluate the efficacy of the OB against TAC-induced mitochondrial nephrotoxicity in CD1 mice. Mice were randomly divided into four groups. Group 1 (control group); administered orally with normal saline (1 mL/kg) for two weeks; Group 2 (OB extract treated-group) (500 mg/kg b...
2018: Toxicology Reports
Matheus Marcon, Ricieri Mocelin, Adrieli Sachett, Anna M Siebel, Ana P Herrmann, Angelo Piato
Background: The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress...
2018: PeerJ
Kévin Jacquet, Sara L Banerjee, François J M Chartier, Sabine Elowe, Nicolas Bisson
Signals from cell surface receptors are often relayed via adaptor proteins. NCK1 and NCK2 are Src-Homology (SH) 2 and 3 domain adaptors that regulate processes requiring a remodelling of the actin cytoskeleton. Evidence from gene inactivation in mouse suggests that NCK1 and NCK2 are functionally redundant, although recent reports support the idea of unique functions for NCK1 and NCK2. We sought to examine this question further by delineating NCK1- and NCK2-specific signalling networks. We used both affinity purification-mass spectrometry and BioID proximity labelling to identify NCK1/2 signalling networks comprised of 98 proteins...
July 12, 2018: Molecular & Cellular Proteomics: MCP
Wenjing Shao, Yuying Li, Feng Chen, Haiyan Jia, Jia Jia, Yan Fu
This study aimed to investigate the role of long non-coding RNA DLEU1 in endometrial cancer (EC) development. The DLEU1 expression in EC tissues and cells (HHUA, KLE, Ishikawa, and ECC-1) were detected. The expression of DLEU1 was suppressed by transfection with sh-DLEU1 and the effects of DLEU1 suppression on the malignant behaviors of Ishikawa cells, including cell viability, apoptosis, migration and migration were then detected. In addition, the interaction of DLEU1 and miR-490 as well as between miR-490 and SP1 in EC were investigated...
July 1, 2018: Die Pharmazie
Shadab Md, Sook Yee Gan, Yong Hong Haw, Chee Liang Ho, Sinrun Wong, Hira Choudhury
Alzheimer's disease (AD) is an increasingly prevalent neurological disorder of the central nervous system. There is growing evidence that amyloidogenesis is a pathological hallmark for AD; this leads to the formation of senile plaques. Naringenin is a bioflavonoid which has neuroprotective effects through its antioxidant and anti-inflammatory properties. However, its clinical usage is limited due to its inefficient transport across biological membranes. In the present study, a naringenin nanoemulsion was prepared and its neuroprotective effects were tested against β-amyloid induced neurotoxicity in a human neuroblastoma cell line (SH-SY5Y)...
July 3, 2018: International Journal of Biological Macromolecules
Andrea Picchianti Diamanti, Luca Navarini, Filippo Messina, Milica Markovic, Luisa Arcarese, Fabio Basta, Giorgia Meneguzzi, Paolo Emanuele Domenico Margiotta, Bruno Laganà, Antonella Afeltra, Raffaele D'Amelio, Annamaria Iagnocco
OBJECTIVES: The ability of ultrasound (US) to identify subclinical joint inflammation in rheumatoid arthritis (RA) patients in remission has been already reported. Nonetheless, current studies present a lack of homogeneity in patient's characteristics and number of joints assessed by US. The aim of this study was to identify a reduced set of target joints to be scanned in RA patients in clinical remission in order to detect subclinical synovitis. METHODS: Forty RA patients in clinical remission (DAS28 ≤2...
June 14, 2018: Clinical and Experimental Rheumatology
Sławomir Lewicki, Aneta Lewicka, Bolesław Kalicki, Agnieszka Sobolewska-Ruta, Bogdan Debski, Robert Zdanowski, Tomasz Syryło, Malgorzata Kloc, Jacek Zbigniew Kubiak
INTRODUCTION: Genistein (plant isoflavone) is a well-known anti-cancer drug with estrogenic-like properties. Genistein also regulates sugar and lipid metabolism; thus, it has anti-diabetic properties. The aim of the study was to evaluate in vitro effects of genistein on glucose transport, fatty acids oxidation, activation of PKB, and expression of genes related to insulin pathway in differentiated myoblast C2C12 mouse cell line. MATERIAL AND METHODS: Differentiated myoblast C2C12 mouse cell line was used to assess the effects of different genistein concentrations on glucose transport and fatty acids oxidation measured by radioactivity technique, activation of PKB, and expression of selected genes related to insulin signaling pathway (IR-α, IR-β, IRS-1, PKB, GLUT-4, PP2A, SH-PTP2) at the mRNA and protein levels...
July 12, 2018: Folia Histochemica et Cytobiologica
Marcos Roberto de Oliveira, Flávia de Bittencourt Brasil, Cristina Ribas Fürstenau
Mitochondria are double-membrane organelles involved in the transduction of energy from different metabolic substrates into adenosine triphosphate (ATP) in mammalian cells. The oxidative phosphorylation system is comprised by the activity of the respiratory chain and the complex V (ATP synthase/ATPase). This system is dependent on oxygen gas (O2 ) in order to maintain a flux of electrons in the respiratory chain, since O2 is the final acceptor of these electrons. Electron leakage from this complex system leads to the continuous generation of reactive species in the cells...
July 11, 2018: Molecular Neurobiology
Cheng-Yang Wang, Huan-Zhang Ding, Xiao Tang, Ze-Geng Li
Background: Chronic obstructive pulmonary disease (COPD) is predicted to become the fifth leading cause of disability and the third leading cause of death around the world by 2020. Though it is potentially treatable and preventable, evidence of brain structural alterations in COPD remains sparse and conflicting. We aim to investigate the effect of Liuweibuqi capsules on CD4+ CD25+ Forkhead box protein 3+ (Foxp3+ ) regulatory T cells (Tregs), helper T cells (Th) and lung function in patients with stable COPD complicated with lung Qi deficiency...
May 2018: Journal of Thoracic Disease
Ana C Marques, Estela N B Busanello, Diogo N de Oliveira, Rodrigo R Catharino, Helena C F Oliveira, Anibal E Vercesi
Statins are the preferred therapy to treat hypercholesterolemia. Their main action consists of inhibiting the cholesterol biosynthesis pathway. Previous studies report mitochondrial oxidative stress and membrane permeability transition (MPT) of several experimental models submitted to diverse statins treatments. The aim of the present study was to investigate whether chronic treatment with the hydrophilic pravastatin induces hepatotoxicity in LDL receptor knockout mice ( LDLr -/- ), a model for human familial hypercholesterolemia...
2018: Frontiers in Pharmacology
Samar Amini, Roland Roberts, Mohammad Raeesi, Zaher Hossein Shomali, Bjorn Lund, Zoya Zarifi
An intraplate earthquake doublet, with 11-min delay between the events, devastated the city of Varzeghan in northwestern Iran on August 11, 2012. The first Mw 6.5 strike-slip earthquake, which occurred after more than 200 years of low seismicity, was followed by an Mw 6.4 oblique thrust event at an epicentral separation of about 6 km. While the first event can be associated with a distinct surface rupture, the absence of a surface fault trace and no clear aftershock signature makes it challenging to identify the fault plane of the second event...
2018: Journal of Seismology
Yi-Hong Ou, Yao-Dong Jiang, Qi Li, Yong-Jiang Zhuang, Qiang Dang, Wan-Long Tan
OBJECTIVE: To investigate the effect of infiltrating mast cells on neuroendocrine differentiation (NED) and docetaxel sensitivity of prostate cancer (PCa) cells in vitro. METHODS: Human PCa cell lines (LNCaP and C4-2) were co-cultured with human mast cell line (HMC-1) in Transwell chambers. Androgen receptor (AR) was silenced in C4-2 cells using sh-AR lentivirus, and p21 was knocked down and overexpressed by transfecting C4-2 cells with pLKO.1-sh-p21 and pCMV-p21, respectively...
June 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Yulin Huang, Jingfu Bao, Xinyi Li, Benfeng Zhang, Gongbin Tang, Tatsuya Omori, Ken-Ya Hashimoto
This paper investigates influence of coupling between Rayleigh and SH SAWs on rotated Y-cut LiNbO3 to their electromechanical coupling. For the purpose, a COM model including the coupling between two SAWs is developed, and the coupling influences to their excitation in addition to electromechanical coupling factors are discussed. Then its validity is confirmed by comparison of its results with the FEM analysis. Comparing with conventional COM shows this extended COM model could fit the complex value area much better in dispersion curve analysis...
May 1, 2018: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Alan C Kubrusly, Miguel A Freitas, Jean Pierre von der Weid, Steve Dixon
Shear horizontally (SH) guided waves, generated by periodic permanent magnet arrays, have been used previously in nondestructive evaluation of metal plates and pipes. When an SH guided wave interacts with a defect or a change in sample thickness, the incident SH wave may undergo mode conversion. Analysis of mode conversion is complicated, due to the interference of several propagating modes in the received signal that can often temporally overlap. This paper proposes a mode selection technique to help understand the interaction of SH guided waves with changes in sample thickness...
July 2018: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Hongyong Song, Beibei Sun, Yueling Liao, Dongliang Xu, Wenzheng Guo, Tong Wang, Bo Jing, Min Hu, Kaimi Li, Feng Yao, Jiong Deng
GPRC5A, a retinoic acid induced gene, is preferentially expressed in lung tissue. Gprc5a gene deletion leads to spontaneous lung tumor development. However, the mechanism of Gprc5a-mediated lung tumor suppression is not fully understood. Here we showed that MDM2, a p53 negative regulator, was dysregulated in Gprc5a-knockout (ko) mouse tracheal epithelial cells (KO-MTEC) compared to wild type (WT) ones. Targeting MDM2 in 1601, a Gprc5a-ko mouse derived lung tumor cell line, and A549, human lung cancer cells, by MDM2 inhibitor Nutlin-3a or small hairpin RNA (sh-RNA), restored p53 signaling pathway, reduced cancer stem cell markers and inhibited tumorigenicity...
July 11, 2018: International Journal of Cancer. Journal International du Cancer
Mariana C Ilha, Paulo F Kramer, Simone H Ferreira, Henrique C Ruschel
Pre-eruptive intracoronal radiolucency (PECR) is characterized by the existence of a radiographic radiolucent area inside the coronal dentin prior to dental eruption. It is a rare clinical entity of unknown etiology, usually asymptomatic and diagnosed through routine radiographs. The aim of this article is to report the treatment of a PECR in an 8-year-old girl. Surgical procedure to expose the crown was conducted; upon tooth eruption, the radiolucent lesion was accessed and the tooth was restored. The case has an 18-month follow-up and emphasis on aspects linked to diagnosis and clinical approach was discussed...
March 2018: International Journal of Clinical Pediatric Dentistry
Juan Zhang, Jia-Cheng Li, Jia-Li Song, Zhi-Qiang Cheng, Ji-Zheng Sun, Cheng-Shi Jiang
A series of new coumarin/1,2,4-oxadiazole hybrids were synthesized and evaluated for cholinesterase inhibitory and neuroprotective activities. Among them, enantiomers 5u and 5v showed potent hBChE inhibitory activity with IC50 values of 8.17 and 9.56 μM, respectively, and also exhibited good selectivity for hBChE over hAChE by 9.49- and 7.58-fold, respectively. In addition, both compounds could protect SH-SY5Y cells against Aβ25-35 -induced neurotoxicity. The preliminary bioassay results provided a new chemotype for multifunctional anti-Alzheimer's disease agents and continuing investigation into compounds 5u and 5v is warranted...
July 10, 2018: Journal of Asian Natural Products Research
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