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Anna Domoslawska, Slawomir Zdunczyk, Monika Franczyk, Marta Kankofer, Tomasz Janowski
The aim of this study was to evaluate the total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. The study was conducted on 10 infertile and 10 fertile dogs of various breeds. Infertility was defined as conception failure at least 3 matings with different bitches. Semen was collected by manual manipulation. The sperm concentration and motility parameters were evaluated using CASA Hamilton Thorne, Vers. IVOS 12.3. The morphology of spermatozoa and the percentage of live and dead sperm cells were assessed microscopically, total antioxidant capacity and the content of SH-groups in seminal plasma were determined spectrophotometrically, the contents of protein peroxidation markers in seminal plasma, bityrosine and formylokinurenine, were determined using spectrofluorimetric methods...
September 17, 2018: Reproduction in Domestic Animals, Zuchthygiene
Zahra Shanesazzade, Maryam Peymani, Kamran Ghaedi, Mohammad Hossein Nasr Esfahani
BACKGROUND: Parkinson's disease (PD) is a neurodegenerative disorder which mainly affects the elderly population of various societies. The main hallmark of this disease is the loss of dopaminergic (DA) neurons. So far, numerous studies have implied the role of microRNAs in fine-tuning cellular processes including apoptosis. Studies have also shown that miR-34a is mainly involved in age-related disorders including Alzheimer's disease, and its expression is usually higher in the brain sample patients...
September 16, 2018: Molecular Genetics & Genomic Medicine
K Menke, M Schwermer, J Felenda, C Beckmann, F Stintzing, A Schramm, T J Zuzak
OBJECTIVES: While Taraxacum officinale (dandelion) extracts showed antitumor effects on adult cancer cells, effects on pediatric tumor cells as a single agent or in combination with mistletoe extracts are hitherto unknown. MATERIAL AND METHODS: The anti-proliferative effects of an aqueous fermented Taraxacum officinale extract (Taraxacum) on a pediatric cancer cell line panel were assessed by cell viability assays (MTT). In two neuroblastoma cell lines, SH-SY5Y and Kelly, the effects on cell cycle distribution (PI staining), mitochondrial integrity (MitoTracker staining), invasion (Boyden chamber assay) and migration (Scratch-assay) as well as the synergistic effects of the co-treatment of Taraxacum and mistletoe preparations (Iscucin® Tiliae or Iscucin® Pini) were investigated...
October 2018: Complementary Therapies in Medicine
Iris Boraschi-Diaz, John S Mort, Dieter Bromme, Yotis A Senis, Alexandra Mazharian, Svetlana V Komarova
The major organic component of bone is collagen type I. Osteoclasts are terminally differentiated multinucleated cells of hematopoietic origin that are essential for physiological development of bone and teeth. We examined if osteoclast differentiation from murine bone marrow precursors is affected by collagen type I, or by its degradation products produced by human recombinant cathepsin K. Osteoclasts formation was dose-dependently inhibited in the presence of full length collagen type I or its 30-75 kDa degradation products added to the osteoclast differentiation media for the duration of an experiment...
September 11, 2018: Bone
Yanling Wang, Junxia Zhao, Cuili Cao, Yongxin Yan, Jing Chen, Fan Feng, Najing Zhou, Shuo Han, Yannan Xu, Juan Zhao, Yunli Yan, Huixian Cui
This study aims to test the role of E2F1-topoIIβ signaling in neuronal differentiation of SH-SY5Y cells. With retinoic acid (RA) induction, a high percentage of cells were found to be arrested at the G0 /G1 phase, with decreased levels of cyclinD1, CDK4, phosphorylation status of pRb and E2F1, in addition to an elevated level of p27. The cells were shown to differentiate into neuronal phenotypes characterized by highly expressed neuronal markers, MAP2 and enriched topoIIβ, and remarkable neurite outgrowth...
July 25, 2018: Differentiation; Research in Biological Diversity
Sara Cappelli, Maurizio Romano, Emanuele Buratti
Heteregeneous ribonucleoproteins (hnRNPs) are a family of RNA-binding proteins that take part in all processes that involve mRNA maturation. As a consequence, alterations of their homeostasis may lead to many complex pathological disorders, such as neurodegeneration and cancer. For many of these proteins, however, their exact function and cellular targets are still not very well known. Here, we focused the attention on two hnRNP family members, hnRNP Q and hnRNP R, that we previously found affecting TDP-43 activity both in Drosophila melanogaster and human neuronal cell line...
2018: Frontiers in Molecular Biosciences
Haitao Wang, Xiaoying Su, Jiankang Fang, Xingan Xin, Xia Zhao, Uma Gaur, Qiang Wen, Jiangping Xu, Peter J Little, Wenhua Zheng
The insulin like growth factor 1 (IGF-1) and its receptor (IGF-1R) facilitate tumor proliferation and progression. Tanshinone IIA (TSN) is an active diterpene quinone isolated from the roots of the herbal plant Salvia miltiorrhiza . TSN inhibits the proliferation of various types of cancer cells but its role in the IGF-1R-induced proliferation of pheochromocytoma (PC12) cells and the potential mechanisms are largely unknown. This study aims to investigate the anti-proliferative effect of TSN in PC12 cells and its role on IGF-1R signaling transduction...
September 12, 2018: International Journal of Molecular Sciences
Alexandre M Colville, Ovidiu D Iancu, Denesa R Lockwood, Priscila Darakjian, Shannon K McWeeney, Robert Searles, Christina Zheng, Robert Hitzemann
The high genetic complexity found in heterogeneous stock (HS-CC) mice, together with selective breeding, can be used to detect new pathways and mechanisms associated with ethanol preference and excessive ethanol consumption. We predicted that these pathways would provide new targets for therapeutic manipulation. Previously (Colville et al., 2017), we observed that preference selection strongly affected the accumbens shell (SH) genes associated with synaptic function and in particular genes associated with synaptic tethering...
2018: Frontiers in Genetics
Roberta Mastrantonio, Veronica D'Ezio, Marco Colasanti, Tiziana Persichini
HIV-associated neurocognitive disorders (HANDs) affect a large part of HIV-infected patients, despite highly active antiretroviral therapy. HANDs occur in the absence of a direct infection of neurons. Nevertheless, viral proteins (e.g., Tat) are capable to cause neuronal dysfunction via oxidative stress, but the cellular pathways leading to HANDs are not yet fully defined. Here, we investigated the effects of Tat on Nrf2-mediated antioxidant response and system xc - expression in U373 human astroglial cells...
September 12, 2018: Molecular Neurobiology
G Y Sánchez Delgado, D Paschoal, H F Dos Santos
Gold complexes are promising compounds used in cancer chemotherapy. Besides their steric features, which enable biomolecule interactions, the redox instability and the high affinity of gold with cellular nucleophiles influence the biological action in these complexes. Both features were herein theoretically investigated for the [Au(C^N^C)Cl] probe complex (C^N^C = 2,6-diphenylpyridine) using H2 O, CH3 SH/CH3 S- , CH3 Se- and meim-4-H (4-methylimidazole) as biomimetic nucleophiles. Based on the results, the lowest energy reaction path followed two consecutive steps: (1) chloride-exchange ([Formula: see text] = 4...
September 12, 2018: Journal of Biological Inorganic Chemistry: JBIC
Mihwa Kim, Liza D Morales, Ik-Soon Jang, Yong-Yeon Cho, Dae Joon Kim
The signal transducer and activator of transcription 3 (STAT3) protein is a major transcription factor involved in many cellular processes, such as cell growth and proliferation, differentiation, migration, and cell death or cell apoptosis. It is activated in response to a variety of extracellular stimuli including cytokines and growth factors. The aberrant activation of STAT3 contributes to several human diseases, particularly cancer. Consequently, STAT3-mediated signaling continues to be extensively studied in order to identify potential targets for the development of new and more effective clinical therapeutics...
September 11, 2018: International Journal of Molecular Sciences
Hendrik Walther, Lakshman N Subbaraman, Lyndon Jones
PURPOSE: To determine the efficacy of multipurpose solutions (MPSs) on the removal of cholesterol deposits from silicone hydrogel (SH) contact lens materials using an in vitro model. MATERIALS AND METHODS: Five SH lens materials: senofilcon A, comfilcon A, balafilcon A, lotrafilcon A, and lotrafilcon B were removed from the blister pack (n=4 for each lens type), incubated for 7 days at 37°C in an artificial tear solution containing C radiolabeled cholesterol. Thereafter, lenses were stored in a preserved saline solution control (Sensitive Eyes Saline Plus) or cleaned with 1 of the 5 MPSs incorporating different preservatives (POLYQUAD/ALDOX, polyquaternium-1/alexidine, polyquaternium-1/PHMB, and 2 based on PHMB alone) using a rub and rinse technique, according to the manufacturer's recommendations, and stored in the MPS for a minimum of 6 hr...
August 30, 2018: Eye & Contact Lens
Yu Cai, Zhenxing Sun, Bokai Liao, Song Zhanqiang, Ting Xiao, Pengfei Zhu
PURPOSE: Treatment of partial-thickness rotator cuff tears (PTRCTs) remains controversial. Few studies have focused on the conservative and new measurements of small to medium PTRCTs. The use of sodium hyaluronate (SH) or platelet-rich plasma (PRP) as a method for rotator cuff repair requires further investigation. The aim of this study was to evaluate the combined use of SH and PRP in the treatment of small to medium PTRCTs.Study designDouble-blinded randomized trial. METHODS: Individuals with PTRCTs detected by clinical examination and magnetic resonance imaging (MRI) were included in this study...
September 7, 2018: Medicine and Science in Sports and Exercise
Basma A Omran, Hussein N Nassar, Sherif A Younis, Nesreen A Fatthallah, A Hamdy, Einas H El-Shatoury, Nour Sh El-Gendy
AIMS: In order to efficiently control the corrosive sulphate-reducing bacteria (SRB); the main precursor of the microbial influenced corrosion (MIC) in oil industry, the ability of Trichoderma longibrachiatum DSMZ 16517 to synthesize silver nanoparticles (AgNPs) was investigated and their biocidal activity against halotolerant SRB was tested. METHODS AND RESULTS: The mycelial cell free filtrate (MCFF) bio-reduced the silver ions (Ag+ ) to their metallic nanoparticle state (Ag0 )...
September 10, 2018: Journal of Applied Microbiology
Antoine Parrot, Sebastian Tavolaro, Guillaume Voiriot, Antony Canellas, Jalal Assouad, Jacques Cadranel, Muriel Fartoukh
Though rare, severe hemoptysis (SH) is associated with a mortality rate exceeding 50% when not managed properly. Areas covered: This paper reviews the recent epidemiological data regarding SH, the role of multidetector computed tomography angiography (MDCTA), and fiberoptic bronchoscopy (FOB) in its management, as well as the value of current treatments. Expert commentary: MDCTA is becoming an essential modality, since it allows determining the location, etiology, and mechanism of the bleeding. FOB can be delayed, except when local control of bleeding is required...
September 10, 2018: Expert Review of Respiratory Medicine
Xiao-Qin Chu, Jie Huang, Zheng-Guang Li, Qian Li, Jiao-Jiao Cao, Jian-Qin Jiang, Shuang-Ying Gui
There has been a growing concern in transdermal drug technology over the past several decades. As a novel transdermal delivery system, Lyotropic liquid crystals (LLC) still face challenges such as drug loading, limited drug permeation and instability of systems. LLC system is so sensitive that a very subtle change in composition may induce a phase transformation or conversion of spatial configuration, and result in a diverse percutaneous delivery subsequently. To find out the effects of hydrophilic and lipophilic components on the structure and transdermal properties of LLCs, hydrophilic sinomenine hydrochloride (SH) and lipophilic cinnamaldehyde (CA) was chosen as a model drug and a skin permeation enhancer, respectively, several formulations were prepared and compared...
September 10, 2018: Current Drug Delivery
Lin-Nan Zhu, Hong-Hua Qiao, Ling Chen, Le-Ping Sun, Jia-Liang Hui, Yong-Ling Lian, Wei-Bing Xie, Jiu-Yang Ding, Yun-le Meng, Bo-Feng Zhu, Ping-Ming Qiu
Methamphetamine (METH) is an illegal and widely abused psychoactive stimulant. METH abusers are at high risk of neurodegenerative disorders, including Parkinson's disease (PD). Previous studies have demonstrated that METH causes alpha-synuclein (α-syn) aggregation in the both laboratory animal and human. In this study, exposure to high METH doses increased the expression of α-syn and the small ubiquitin-related modifier 1 (SUMO-1). Therefore, we hypothesized that SUMOylation of α-syn is involved in high-dose METH-induced α-syn aggregation...
2018: Frontiers in Cellular Neuroscience
Shi-Kun Guo, Ming-Feng Shen, Hong-Wei Yao, Yong-Sheng Liu
BACKGROUND/AIMS: Transforming growth factor beta-induced protein (TGFBI) is an extracellular matrix protein induced by TGF-β. Previous studies have reported that the abnormal expression of TGFBI is related to the occurrence and development of some types of cancers, while the role of TGFBI in glioma is uncertain. METHODS: The association between TGFBI expression and the prognosis of patients with glioma was analyzed based on data obtained from The Cancer Genome Atlas database...
September 7, 2018: Cellular Physiology and Biochemistry
Carolina Galarza Vargas, Jeferson Delgado da Silva Junior, Thallita Kelly Rabelo, José Cláudio Fonseca Moreira, Daniel Pens Gelain, Eliseu Rodrigues, Paula Rossini Augusti, Alessandro de Oliveira Rios, Simone Hickmann Flôres
Rice bran is obtained from the rice polishing process, and this by-product contains many bioactive compounds. In this study, the composition of phenolic compounds from red and black rice brans was determined by HPLC-DAD-MS. Additionally, the neuroprotective ability of these brans in SH-SY5Y cells insulted with hydrogen peroxide (H2 O2 ) was evaluated. The phenolic constituents of rice bran were separated into hydrophilic and pellet fractions. The major phenolic compound in both samples was ferulic acid. Cyanidin 3-glucoside was the main anthocyanin in black rice bran...
November 2018: Food Research International
Bruno Tota, Daniela Amelio, Maria Carmela Cerra, Filippo Garofalo
This review aims to summarize the changes of the NOS/NO system which occur in the lungs, gills, kidney, heart, and myotomal muscle of air breathing fish of the genus Protopterus, i.e. P. dolloi and P. annectens, in relation to the switch from freshwater to aestivation, and vice-versa. The modifications of NOS and its partners Akt and Hsp-90, and HIF-1α, detected by immunohistochemical and molecular biology methods, are discussed together with the apoptosis rate, evaluated by TUNEL. We hypothesize that these molecular components are key elements of the stress-induced signal transduction/integration networks which allow the lungfish to overcome the dramatic environmental challenges experienced at the beginning, during, and at the end of the dry season...
September 5, 2018: Acta Histochemica
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