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https://www.readbyqxmd.com/read/29109701/microbial-community-and-biochemical-dynamics-of-biological-soil-crusts-across-a-gradient-of-surface-coverage-in-the-central-mojave-desert
#1
Rakesh Mogul, Parag Vaishampayan, Mina Bashir, Chris P McKay, Keith Schubert, Rosalba Bornaccorsi, Ernesto Gomez, Sneha Tharayil, Geoffrey Payton, Juliana Capra, Jessica Andaya, Leonard Bacon, Emily Bargoma, David Black, Katie Boos, Michaela Brant, Michael Chabot, Danny Chau, Jessica Cisneros, Geoff Chu, Jane Curnutt, Jessica DiMizio, Christian Engelbrecht, Caroline Gott, Raechel Harnoto, Ruben Hovanesian, Shane Johnson, Britne Lavergne, Gabriel Martinez, Paul Mans, Ernesto Morales, Alex Oei, Gary Peplow, Ryan Piaget, Nicole Ponce, Eduardo Renteria, Veronica Rodriguez, Joseph Rodriguez, Monica Santander, Khamille Sarmiento, Allison Scheppelmann, Gavin Schroter, Devan Sexton, Jenin Stephenson, Kristin Symer, Tatiane Russo-Tait, Bill Weigel, Mary B Wilhelm
In this study, we expand upon the biogeography of biological soil crusts (BSCs) and provide molecular insights into the microbial community and biochemical dynamics along the vertical BSC column structure, and across a transect of increasing BSC surface coverage in the central Mojave Desert, CA, United States. Next generation sequencing reveals a bacterial community profile that is distinct among BSCs in the southwestern United States. Distribution of major phyla in the BSC topsoils included Cyanobacteria (33 ± 8%), Proteobacteria (26 ± 6%), and Chloroflexi (12 ± 4%), with Phormidium being the numerically dominant genus...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29089518/diversity-oriented-biosynthesis-via-accelerated-evolution-of-modular-gene-clusters
#2
Aleksandra Wlodek, Steve G Kendrew, Nigel J Coates, Adam Hold, Joanna Pogwizd, Steven Rudder, Lesley S Sheehan, Sarah J Higginbotham, Anna E Stanley-Smith, Tony Warneck, Mohammad Nur-E-Alam, Markus Radzom, Christine J Martin, Lois Overvoorde, Markiyan Samborskyy, Silke Alt, Daniel Heine, Guy T Carter, Edmund I Graziani, Frank E Koehn, Leonard McDonald, Alexander Alanine, Rosa María Rodríguez Sarmiento, Suzan Keen Chao, Hasane Ratni, Lucinda Steward, Isobel H Norville, Mitali Sarkar-Tyson, Steven J Moss, Peter F Leadlay, Barrie Wilkinson, Matthew A Gregory
Erythromycin, avermectin and rapamycin are clinically useful polyketide natural products produced on modular polyketide synthase multienzymes by an assembly-line process in which each module of enzymes in turn specifies attachment of a particular chemical unit. Although polyketide synthase encoding genes have been successfully engineered to produce novel analogues, the process can be relatively slow, inefficient, and frequently low-yielding. We now describe a method for rapidly recombining polyketide synthase gene clusters to replace, add or remove modules that, with high frequency, generates diverse and highly productive assembly lines...
October 31, 2017: Nature Communications
https://www.readbyqxmd.com/read/29079920/redefining-the-ki-67-index-stratification-for-low-grade-pancreatic-neuroendocrine-tumors-improving-its-prognostic-value-for-recurrence-of-disease
#3
Alexandra G Lopez-Aguiar, Cecilia G Ethun, Lauren M Postlewait, Kristen Zhelnin, Alyssa Krasinskas, Bassel F El-Rayes, Maria C Russell, Juan M Sarmiento, David A Kooby, Charles A Staley, Shishir K Maithel, Kenneth Cardona
BACKGROUND: The Ki-67 index is an established prognostic marker for recurrence after resection of pancreatic neuroendocrine tumors (PanNETs) that groups tumors into three categories: low grade (< 3%), intermediate grade (3-20%), and high grade (> 20%). Given that the majority of resected PanNETs have a Ki-67 less than 3%, this study aimed to stratify this group further to predict disease recurrence more accurately. METHODS: The Ki-67 index was pathologically re-reviewed and scored by a pathologist blinded to all other clinicopathologic variables using tissue microarray blocks made in triplicate...
October 27, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/29078671/use-of-autologous-platelet-rich-plasma-prp-in-stopping-massive-hemoptysis-at-the-lung-center-of-the-philippines-a-pilot-study
#4
Armand Gregorio C Sarmiento, Jose Luis J Danguilan, Zenaida M Mariano, Maria Teresa A Barzaga
BACKGROUND: The purpose of this study is to determine the effect of using autologous platelet rich plasma (PRP) in patients having massive hemoptysis within a period of one week. METHODS: This is a prospective cohort study involving 20 consecutive patients admitted who met the criteria for massive hemoptysis from July to October 2011. After stabilizing the patient, fiberoptic bronchoscopy (FOB) was performed for localization of bleeding within 6 hours from diagnosis...
2017: J Vis Surg
https://www.readbyqxmd.com/read/29069487/nf-%C3%AE%C2%BAb1-ikk%C3%AE%C2%B5-gene-expression-and-clinical-activity-in-patients-with-rheumatoid-arthritis
#5
Fabiola Lilí Sarmiento Salinas, Jonnathan Guadalupe Santillán Benítez, María Dolores Hernández Navarro, Hugo Mendieta Zerón
Objective: Rheumatoid arthritis (RA) is a systemic and autoimmune disorder whose primary characteristic is the chronic inflammation of joints. The objective of this study was to evaluate whether there was an association between nuclear factor kappa beta1/IKK epsilon (NF-κB1/IKKε) gene expression and clinical activity in RA. Methods: Sixty patients with RA were included in the study: 30 with clinical activity and 30 with clinical remission. NF-κB1/IKKε gene expression was performed by real-time quantitative polymerase chain reaction through relative quantification with Taqman probes...
October 23, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/29066246/changes-on-serum-and-hepatic-lipidome-after-a-chronic-cadmium-exposure-in-wistar-rats
#6
Victor Enrique Sarmiento-Ortega, Samuel Treviño, José Ángel Flores-Hernández, Patricia Aguilar-Alonso, Diana Moroni-González, Violeta Aburto-Luna, Alfonso Diaz, Eduardo Brambila
No abstract text is available yet for this article.
December 1, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29058616/correction-to-novel-snp-markers-in-invge-and-sssi-genes-are-associated-with-natural-variation-of-sugar-contents-and-frying-color-in-solanum-tuberosum-group-phureja
#7
D Duarte-Delgado, D Juyó, C Gebhardt, F Sarmiento, T Mosquera-Vásquez
No abstract text is available yet for this article.
October 20, 2017: BMC Genetics
https://www.readbyqxmd.com/read/29058557/epidemiology-of-traumatic-spinal-cord-injury-in-childhood-and-adolescence-in-galicia-spain-report-of-the-last-26-years
#8
E Canosa-Hermida, R Mora-Boga, J J Cabrera-Sarmiento, M E Ferreiro-Velasco, S Salvador-de la Barrera, A Rodríguez-Sotillo, A Montoto-Marqués
OBJECTIVE: To analyze the characteristics of traumatic spinal cord injury in children of Galicia (Spain). DESIGN: Descriptive and retrospective study. METHODS: Data extracted from the internal registry of the Spinal Cord Injury Unit and the patient's medical records, between March 1988 and December 2014. INCLUSION CRITERIA: patients aged ≤ 17 years with a traumatic spinal cord injury. OUTCOME MEASURES: Total patients, percentages, incidence, ASIA scale results and improvement...
October 23, 2017: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/29045705/outbreak-and-eradication-of-tropical-rat-mite-acari-macronyssidae-in-a-european-animal-facility
#9
Yeray Brito-Casillas, Mercedes Díaz-Sarmiento, Moisés García-Arencibia, Cristina Carranza, Antonio Castrillo, Leandro Fernández-Pérez, Manuel Zumbado-Peña, Jorge F González, Ana M Wägner
A zoonotic, opportunistic out-break of tropical rat mite Ornithonyssus bacoti [Acari: Macronyssidae; Ornithonyssus bacoti (Hirst)] in an animal facility, is described. Immunocompetent mice [Mus musculus (Linnaeus)] and rat [Rattus norvegicus (Berkenhout)] strains in a conventional health status facility suffered from scratching and allopecia and staff members suffered from pruritic, erythemato-papular lesions, presumed to be allergic in origin. O. bacoti was identified and treatment with a 0.1% ivermectin solution led to its complete erradication...
October 17, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29032930/3-hydroxy-3-4-dimethoxyflavone-blocks-tubulin-polymerization-and-is-a-potent-apoptotic-inducer-in-human-sk-mel-1-melanoma-cells
#10
Francisco Estévez-Sarmiento, Mercedes Said, Ignacio Brouard, Francisco León, Celina García, José Quintana, Francisco Estévez
Flavonoids are naturally occurring polyphenolic compounds and are among the most promising anticancer agents. A series of flavonols and their 3-methyl ether derivatives were synthesized and assessed for cytotoxicity. It was found that 3'-hydroxy-3,4'-dimethoxyflavone (flavonoid 7a) displayed strong cytotoxicity against human SK-MEL-1 melanoma cells and blocked tubulin polymerization, but had no significant cytotoxic effects against quiescent or proliferating human peripheral blood mononuclear cells. Our analyses showed that flavonoid 7a induces G2-M cell cycle arrest and apoptosis in melanoma cells which is associated with cytochrome c release and activation of both extrinsic and intrinsic apoptotic pathways of cell death...
November 1, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29032844/-family-adherence-in-serious-mental-disorder
#11
Ernesto Martín Padilla, Diana Obando Posada, Pedro Sarmiento Medina
OBJECTIVE: Identify attitudes and behaviors that evidence and characterize family adherence to treatment in patients with severe mental disorder. DESIGN: Qualitative descriptive, from an interpretative social approach. LOCATION: Chia, Colombia, with professionals in the psychiatric and geriatric settings. PARTICIPANTS: Twelve professionals in psychiatry, nursing and psychology, with experience in care of patients with serious mental disorder and their families...
October 9, 2017: Atencion Primaria
https://www.readbyqxmd.com/read/29030970/annexin-a1-annexin-a2-and-dyrk-1b-are-upregulated-during-gas1-induced-cell-cycle-arrest
#12
G Pérez-Sánchez, A Jiménez, M A Quezada-Ramírez, E Estudillo, A E Ayala-Sarmiento, G Mendoza-Hernández, Justino Hernández-Soto, F C Hernández-Hernández, F E Cázares-Raga, J Segovia
GAS1 is a pleiotropic protein that has been investigated because of its ability to induce cell proliferation, cell arrest, and apoptosis, depending on the cellular or the physiological context in which it is expressed. At this point, we have information about the molecular mechanisms by which GAS1 induces proliferation and apoptosis; but very few studies have been focused on elucidating the mechanisms by which GAS1 induces cell arrest. With the aim of expanding our knowledge on this subject, we first focused our research on finding proteins that were preferentially expressed in cells arrested by serum deprivation...
October 14, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29029632/fatal-haemorrhage-and-neoplastic-thrombosis-in-a-captive-african-lion-panthera-leo-with-metastatic-testicular-sex-cord-stromal-tumour
#13
Omar Antonio Gonzales-Viera, Angélica María Sánchez-Sarmiento, Natália Coelho Couto de Azevedo Fernandes, Juliana Mariotti Guerra, Rodrigo Albergaria Ressio, José Luiz Catão-Dias
BACKGROUND: The study of neoplasia in wildlife species contributes to the understanding of cancer biology, management practices, and comparative pathology. Higher frequencies of neoplasms among captive non-domestic felids have been reported most commonly in aging individuals. However, testicular tumours have rarely been reported. This report describes a metastatic testicular sex cord-stromal tumour leading to fatal haemorrhage and thrombosis in a captive African lion (Panthera leo). CASE PRESENTATION: During necropsy of a 16-year-old male African lion, the left testicle and spermatic cord were found to be intra-abdominal (cryptorchid), semi-hard and grossly enlarged with multiple pale-yellow masses...
October 13, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29016404/hepatobiliary-mucinous-cystic-neoplasms-with-ovarian-type-stroma-so-called-hepatobiliary-cystadenoma-cystadenocarcinoma-clinicopathologic-analysis-of-36-cases-illustrates-rarity-of-carcinomatous-change
#14
Brian Quigley, Michelle D Reid, Burcin Pehlivanoglu, Malcolm H Squires, Shishir Maithel, Yue Xue, Choi Hyejeong, Gizem Akkas, Takashi Muraki, David A Kooby, Juan M Sarmiento, Ken Cardona, Aarti Sekhar, Alyssa Krasinskas, Volkan Adsay
The literature is highly conflicting on hepatobiliary mucinous cystic neoplasms (MCNs), aka "hepatobiliary cystadenoma/cystadenocarcinoma," largely because ovarian stroma (OS) was not a requirement until WHO-2010 and is not widely applied even today. In this study, MCNs (with OS) accounted for 24 of 229 (11%) resected hepatic cysts in one institution. Eight of the 32 (25%) cysts that had been originally designated as hepatobiliary cystadenoma/cystadenocarcinoma at the time of diagnosis proved not to have an OS during this review and were thus re-classified as non-MCN...
October 12, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28973927/soilborne-fungi-have-host-affinity-and-host-specific-effects-on-seed-germination-and-survival-in-a-lowland-tropical-forest
#15
Carolina Sarmiento, Paul-Camilo Zalamea, James W Dalling, Adam S Davis, Simon M Stump, Jana M U'Ren, A Elizabeth Arnold
The Janzen-Connell (JC) hypothesis provides a conceptual framework for explaining the maintenance of tree diversity in tropical forests. Its central tenet-that recruits experience high mortality near conspecifics and at high densities-assumes a degree of host specialization in interactions between plants and natural enemies. Studies confirming JC effects have focused primarily on spatial distributions of seedlings and saplings, leaving major knowledge gaps regarding the fate of seeds in soil and the specificity of the soilborne fungi that are their most important antagonists...
October 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28972702/survival-outcomes-for-patients-with-localized-upper-tract-urothelial-carcinoma-managed-with-non-definitive-treatment
#16
Jamil S Syed, Kevin A Nguyen, Alfredo Suarez-Sarmiento, Katelyn Johnson, Michael S Leapman, Jay D Raman, Brian Shuch
OBJECTIVE: To investigate the outcomes of patients with upper tract urothelial carcinoma (UTUC) with non-definitive therapy, which currently remains unknown. SUBJECTS/PATIENTS AND METHODS: We utilized the Surveillance, Epidemiology, and End Results (SEER) database to identify individuals with a localized, histologically confirmed kidney/renal pelvis and ureteral urothelial carcinoma. Survival analysis using the Kaplan Meier method was performed. A competing risk model evaluated the cumulative incidence and predictors of cancer specific mortality (CSM)...
October 3, 2017: BJU International
https://www.readbyqxmd.com/read/28971901/molecular-and-clinical-profile-of-von-willebrand-disease-in-spain-pcm-evw-es-comprehensive-genetic-analysis-by-next-generation-sequencing-of-480-patients
#17
Nina Borràs, Javier Batlle, Almudena Pérez-Rodríguez, María Fernanda López-Fernández, Ángela Rodríguez-Trillo, Esther Lourés, Ana Rosa Cid, Santiago Bonanad, Noelia Cabrera, Andrés Moret, Rafael Parra, María Eva Mingot-Castellano, Ignacia Balda, Carme Altisent, Rocío Pérez-Montes, Rosa María Fisac, Gemma Iruín, Sonia Herrero, Inmaculada Soto, Beatriz de Rueda, Victor Jimimenez-Yuste, Nieves Alonso, Dolores Vilariño, Olga Arija, Rosa Campos, María José Paloma, Nuria Bermejo, Rubén Berrueco, José Mateo, Karmele Arribalzaga, Pascual Marco, Ángeles Palomo, Lizheidy Sarmiento, Belén Iñigo, María Del Mar Nieto, Rosa Vidal, María Paz Martínez, Reyes Aguinaco, Jesús María César, María Ferreiro, Javier García-Frade, Ana María Rodríguez-Huerta, Jorge Cuesta, Ramón Rodríguez-González, Faustino García-Candel, Rosa Cornudella, Carlos Aguilar, Francisco Vidal, Irene Corrales
Molecular diagnosis of patients with von Willebrand disease is pending in most populations due to the complexity and high cost of conventional molecular analyses. The need for molecular and clinical characterization of von Willebrand disease in Spain prompted the creation of a multicenter project (PCM-EVW-ES) that resulted in the largest prospective cohort study of patients with all types of von Willebrand disease. Molecular analysis of relevant regions of the VWF, including intronic and promoter regions, was achieved in the 556 individuals recruited by development of a simple, innovative, relatively low-cost protocol based on microfluidic technology and next-generation sequencing...
September 29, 2017: Haematologica
https://www.readbyqxmd.com/read/28966800/parity-history-determines-a-systemic-inflammatory-response-to-spread-of-ovarian-cancer-in-naturally-aged-mice
#18
Ulises Urzua, Carlos Chacon, Luis Lizama, Sebastián Sarmiento, Pía Villalobos, Belén Kroxato, Katherine Marcelain, María-Julieta Gonzalez
Aging intersects with reproductive senescence in women by promoting a systemic low-grade chronic inflammation that predisposes women to several diseases including ovarian cancer (OC). OC risk at menopause is significantly modified by parity records during prior fertile life. To date, the combined effects of age and parity on the systemic inflammation markers that are particularly relevant to OC initiation and progression at menopause remain largely unknown. Herein, we profiled a panel of circulating cytokines in multiparous versus virgin C57BL/6 female mice at peri-estropausal age and investigated how cytokine levels were modulated by intraperitoneal tumor induction in a syngeneic immunocompetent OC mouse model...
October 2017: Aging and Disease
https://www.readbyqxmd.com/read/28945994/efficacy-of-empathy-training-in-nursing-students-a-quasi-experimental-study
#19
Pilar Bas-Sarmiento, Martina Fernández-Gutiérrez, María Baena-Baños, Jose Manuel Romero-Sánchez
BACKGROUND: Empathy is a competency to be learned by nurses and a therapeutic tool in the helping relationship that has repercussions on the health of both patients and professionals. OBJECTIVES: To determine the efficacy of an experiential training for improving the empathy of nursing students in terms of capacity building, empathic performance and increased learning perception and retention of the material. DESIGN: A quasi-experimental study of a single group with pretest-posttest measurements of the educational intervention and follow-up at one month after the training...
December 2017: Nurse Education Today
https://www.readbyqxmd.com/read/28945270/whole-brain-radiation-therapy-wbrt-alone-versus-wbrt-and-radiosurgery-for-the-treatment-of-brain-metastases
#20
REVIEW
Chirag G Patil, Katie Pricola, J Manuel Sarmiento, Sachin K Garg, Andrew Bryant, Keith L Black
BACKGROUND: Historically, whole brain radiation therapy (WBRT) has been the main treatment for brain metastases. Stereotactic radiosurgery (SRS) delivers high-dose focused radiation and is being increasingly utilized to treat brain metastases. The benefit of adding SRS to WBRT is unclear. This is an updated version of the original Cochrane Review published in Issue 9, 2012. OBJECTIVES: To assess the efficacy of WBRT plus SRS versus WBRT alone in the treatment of adults with brain metastases...
September 25, 2017: Cochrane Database of Systematic Reviews
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