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Weiguo Zhang, Bin Qiao, Junli Fan
We aimed to elucidate the roles and regulatory mechanism of miR-4443 in regulating the resistance of non-small cell lung cancer (NSCLC) cells to epirubicin (EPI). Fifty-four advanced NSCLC patients were classified as ''insensitive'' or ''sensitive'' according to patient's responses following EPI-based chemotherapy and then the expression of miR-4443 was determined. The EPI-resistant H1299 cells were collected and transfected with miR-4443 mimics, whereas parental H1299 cells were transfected with miR-4443 inhibitors...
July 1, 2018: Die Pharmazie
Lei Chen, Biwu Liu, Zhang-Run Xu, Juewen Liu
Selective extraction of a small amount of nucleic acids from complex biological samples containing a high concentration of proteins is critical for bioanalytical chemistry. A number of previously published studies have focused on long, double-stranded DNA such as plasmid DNA. On the other hand, we are interested in short oligonucleotides. Nucleic acids have a negatively charged phosphate backbone that interacts with metal oxides strongly, and this may be used to distinguish them from proteins. In this work, a few metal oxide nanoparticles were screened including NiO, CoO, ZnO, TiO2, CeO2, and Fe3O4 for DNA recovery...
July 12, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
G Ramakrishna, Parampreet Kaur, Deepti Nigam, Pavan K Chaduvula, Sangita Yadav, Akshay Talukdar, Nagendra Kumar Singh, Kishor Gaikwad
BACKGROUND: Water permeability governed by seed coat is a major facet of seed crops, especially soybean, whose seeds lack physiological dormancy and experience rapid deterioration in seed viability under prolonged storage. Moreover, the physiological and chemical characteristics of soybean seeds are known to vary with seed coat color. Thus, to underpin the genes controlling water permeability in soybean seeds, we carried out an in-depth characterization of the associated genomic variation...
July 9, 2018: BMC Plant Biology
Megumu Sakoda, Makoto Kaneko, Seiichi Ohta, Pan Qi, Shigetoshi Ichimura, Yutaka Yatomi, Taichi Ito
We have developed a new hydrogel hemostat composed of hyaluronan (HA) conjugated with inorganic polyphosphate (PolyP). A hemostatic hydrogel, HAX-PolyP, was formed rapidly by mixing aldehyde-modified HA and hydrazide-modified HA conjugated with PolyP (HA-PolyP). Although the gelation rate decreased with increasing PolyP content, the gelation time was below 5 min. In addition, the hydrogel swelling volume decreased with increasing PolyP content, but the degradation rate did not depend on PolyP content and the hydrogel underwent complete degradation through hydrolysis over 3 weeks in phosphate buffered saline...
July 9, 2018: Biomacromolecules
Guangxin Feng, Yinong Feng, Tengjiao Guo, Yisheng Yang, Wei Guo, Min Huang, Haohao Wu, Ming-Yong Zeng
Polyphosphates are one of the active compounds from probiotics to maintain gut health. The current research extracted and purified intact biogenic polyphosphate nanoparticles (BPNPs) from Synechococcus sp. PCC 7002 cells. BPNPs were near-spherical anionic particles (56.9 ± 15.1 nm) mainly composed by calcium and magnesium salt of polyphosphate, and were colloidally stable at near-neutral and alkaline pH. BPNPs survived gastrointestinal digestion in mice, and could be absorbed and transported by polarized Caco-2 cell monolayers...
July 5, 2018: Journal of Agricultural and Food Chemistry
Shu-Ying Geng, Wei-Zhang Fu, Jing Wang, Shu-Lian Zheng
The application of SBR reactors is becoming more common and in order to further improve the efficiency of their treatment it has been proposed to add a magnetic field to SBR systems. The effects of different magnetic fields on the degradation efficiency of conventional pollutants in an SBR was studied. MiSeq high-throughput sequencing techniques were used to analyze the diversity of microbial communities in activated sludge under magnetic field conditions. These results show that the addition of a magnetic field improves the performance of a SBR system to an extent and magnetic induction effects are most obvious at 7×10-2 T...
November 8, 2017: Huan Jing Ke Xue= Huanjing Kexue
Jing-Feng Gao, Shi-Jie Wang, Xiao-Yan Fan, Kai-Ling Pan, Li-Fang Zhang, Shu-Jun Zhang, Yong-Qing Gao, Shuai Zhang
In this study, domestic sewage was utilized to cultivate aerobic granular sludge (AGS) in a simultaneous nitrogen and phosphorus removal (SNPR) system. The bacterial population dynamics during the aerobic sludge granulation were investigated to reveal the granulation mechanisms using Illumina MiSeq PE300 high-throughput sequencing. Quantitative real time polymerase chain reactions (PCR) were used to investigate shifts in the abundance of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), nitrite-oxidizing bacteria (NOB) and polyphosphate accumulating organisms (PAOs)...
November 8, 2017: Huan Jing Ke Xue= Huanjing Kexue
Chang-Yun Teng, Jian-Guo Shen, Zhong Wang, Hang Wang, Hong-Yi Li, Zhi-Jian Zhang
Microbial community and phosphorus forms in response to simulated climate warming were studied by high-throughput sequencing and 31 P nuclear magnetic resonance(31 P-NMR) respectively, which were from wetland soils in constructed microcosm columns. The results revealed that relative abundances of Firmicutes, Clostridia, Clostridiales, Clostridiaceae and Clostridium were significantly decreased by 65%-98%, 69%-87%, 67%-87%, 73%-97% and 74%-93% under warming condition respectively, suggesting warming had a significant inhibitory effect on the bacterial lineage from Firmicutes to Clostridium at different taxonomic level...
July 8, 2017: Huan Jing Ke Xue= Huanjing Kexue
Mansi Punjabi, Navneeta Bharadvaja, Monica Jolly, Anil Dahuja, Archana Sachdev
Soybean is one of the leading oilseed crop in the world and is showing a remarkable surge in its utilization in formulating animal feeds and supplements. Its dietary consumption, however, is incongruent with its existing industrial demand due to the presence of anti-nutritional factors in sufficiently large amounts. Phytic acid in particular raises concern as it causes a concomitant loss of indigestible complexed minerals and charged proteins in the waste and results in reduced mineral bioavailability in both livestock and humans...
2018: Frontiers in Plant Science
Bin Zhang, Weidong Wang, Chonghui Li, Rong Liu
The inositol polyphosphate-4-phosphatase type II (INPP4B) has been mostly proposed to act as a tumor suppressor whose expression is frequently dysregulated in numerous human cancers. To date, little is unveiled about whether and how INPP4B will exert its tumor suppressive function on the turnover of cadherin-based cell-cell adhesion system in pancreatic ductal adenocarcinomas (PDACs) in vitro. Here we provide the evidence that INPP4B manipulates cadherin switch in certain PDAC cell lines through a phosphorylated AKT-inactivation manner...
June 28, 2018: Cell Adhesion & Migration
Yang Xiang, Xudong Ru, Jinguo Shi, Jiang Song, Haidong Zhao, Yaqing Liu, Guizhe Zhao
A new granular slow-release fertilizer prepared by a cold extrusion strategy (GSRFEx) based on urea-formaldehyde (UF), ammonium polyphosphate (APP) and amorphous silica gel (ASG) was presented. Characterizations showed that there were a strong hydrogen bond interaction and good compatibility among UF, APP and ASG in GSRFEx. Mechanical properties as well as slow-release properties of GSRFEx were greatly enhanced after adding APP and ASG into UF. Rape pot experiments indicated that GSRFEx could improve N use efficiency dramatically and thereby facilitate the growth of rapes...
June 26, 2018: Journal of Agricultural and Food Chemistry
Yueyun Li, Helen A Cope, Sheikh Mokhlesur Rahman, Guangyu Li, Per Halkjær Nielsen, Alistair Philip David Elfick, April Z Gu
This study reports a proof-of concept study to demonstrate the novel approach of phenotyping microbial communities in enhanced biological phosphorus removal (EBPR) systems using single cell Raman microspectroscopy and link it with phylogentic structures. We uses hierarchical clustering analysis (HCA) of single-cell Raman spectral fingerprints and intracellular polymers signatures to separate and classify the functionally relevant populations in EBPR systems, namely polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs), as well as other microbial populations...
June 26, 2018: Environmental Science & Technology
Kaitlyn L Norman, Christian A Shively, Amberlene J De La Rocha, Nebibe Mutlu, Sukanya Basu, Paul J Cullen, Anuj Kumar
Pseudohyphal growth is a nutrient-regulated program in which budding yeast form multicellular filaments of elongated and connected cells. Filamentous growth is required for virulence in pathogenic fungi and provides an informative model of stress-responsive signaling. The genetics and regulatory networks modulating pseudohyphal growth have been studied extensively, but little is known regarding the changes in metabolites that enable pseudohyphal filament formation. Inositol signaling molecules are an important class of metabolite messengers encompassing highly phosphorylated and diffusible inositol polyphosphates (InsPs)...
June 25, 2018: PLoS Genetics
Yun Zhang, Yang Zhang, Jie Gao, Qiuxuan Shen, Zhihui Bai, Xuliang Zhuang, Guoqiang Zhuang
Although Nitrobacter winogradskyi is an important chemoorganotrophic organism for the study of nitrite-oxidizing bacteria (NOB) physiology as well as nitrification, until now, the mixotrophic medium for this organism growth has not been optimized, comprehensively. In this study, we aimed to improve the growth medium of N. winogradskyi using the one-factor-at-a-time (NaNO2 , glycerol, pH) method. In addition, a further experimental design was carried out based on Central Composite Design (CCD) with response surface methodology (RSM)...
June 21, 2018: Letters in Applied Microbiology
Indranil Biswas, Sumith R Panicker, Xiaofeng Cai, Padmaja Mehta-D'souza, Alireza R Rezaie
OBJECTIVE: Inorganic polyphosphate (polyP) is known to modulate coagulation, inflammation, and metabolic pathways. It also amplifies inflammatory responses of HMGB1 (high mobility group box 1) in endothelial cells. The objective of this study was to evaluate the effect of polyP on von Willebrand factor (VWF) release from endothelial cells with or without HMGB1. APPROACH AND RESULTS: EA.hy926 endothelial cells were treated with different concentrations of polyP70 alone or in combination with different concentrations of HMGB1...
June 21, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
Cristina Azevedo, Jyoti Singh, Nicole Steck, Alexandre Hofer, Felix A Ruiz, Tanya Singh, Henning J Jessen, Adolfo Saiardi
Phenotypes are established by thigh regulation on protein functions. This regulation can be mediated allosterically, through protein binding, and covalently, through post-translational modification (PTM). The integration of an ever-increasing number of PTMs into regulatory networks enable and define the proteome complexity. Protein PTMs can occur enzymatically and non-enzymatically. Polyphosphorylation, a recently discovered PTM that belongs to the latter category, is the covalent attachment of the linear ortho-phosphate polymer called inorganic polyphosphate (polyP) to lysine residues...
June 20, 2018: ACS Chemical Biology
Chun-Yang Sun, Ziyang Cao, Xue-Jun Zhang, Rong Sun, Chun-Shui Yu, Xianzhu Yang
The simple integration of chemotherapeutic drugs and photosensitizers (PSs) into the same nanocarriers only achieves a combination of chemo-photodynamic therapy but may not confer synergistic effects. The boosted intracellular release of chemotherapeutic drugs during the photodynamic therapy (PDT) process is necessary to achieve a cascade of amplified synergistic therapeutic effects of chemo-photodynamic therapy. Methods: In this study, we explored an innovative hyperbranched polyphosphate (RHPPE) containing a singlet oxygen (SO)-labile crosslinker to boost drug release during the PDT process...
2018: Theranostics
Xiuzhong Wang, Anand Lopez, Juewen Liu
Phosphate containing molecules exist in many forms in biology and environment, and their interaction with metal oxides is an important aspect of their chemistry and biochemistry. In this work, phosphates with different degrees of polymerization (e.g. orthophosphate, pyrophosphate, sodium triphosphate (STPP), trimetaphosphate (STMP), and polyphosphate with 25 phosphate units) and phosphates with one or two capping groups were studied. CeO2 nanoparticles (nanoceria) were used as a model metal oxide. DNA is also a polyphosphate, and a fluorescently labeled DNA oligonucleotide was mixed with nanoceria...
June 11, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Wei Zhou, Rui Huang, Zhiguang Zhu, Yi-Heng P Job Zhang
Thermostability and specific activity of enzymes are two of the most important properties for industrial biocatalysts. Here we developed a Petri dish-based double-layer high-throughput screening (HTS) strategy for rapid identification of desired mutants of polyphosphate glucokinase (PPGK) from a thermophilic actinobacterium Thermobifida fusca YX with both enhanced thermostability and activity. Escherichia coli colonies representing a PPGK mutant library were grown on the first-layer Phytagel™-based plates, which can remain solid for 1 h even at heat treatment temperatures of more than 100 °C...
June 8, 2018: Applied and Environmental Microbiology
Meng Zhang, Hui Tian, Rongrong Li, Wenlong Yan, Rongxue Xu
This study aimed to investigate the key roles of miR-4286 in regulating the development of esophageal carcinoma, as well as its regulatory mechanism. The expression of miR-4286 in esophageal carcinoma tissues and cells was determined. Effects of abnormal expression of miR-4286 cell viability, apoptosis, migration and invasion were further investigated. Furthermore, the potential target of miR-4286 was explored, and the regulatory relationship between miR-4286 and Janus tyrosine kinase/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway was elucidated...
June 1, 2018: Die Pharmazie
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