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https://www.readbyqxmd.com/read/28818689/sol-gel-derived-hydroxyapatite-films-over-porous-calcium-polyphosphate-substrates-for-improved-tissue-engineering-of-osteochondral-like-constructs
#1
Whitaik David Lee, Rahul Gawri, Robert M Pilliar, William L Stanford, Rita A Kandel
Integration of in vitro-formed cartilage on a suitable substrate to form tissue-engineered implants for osteochondral defect repair is a considerable challenge. In healthy cartilage, a zone of calcified cartilage (ZCC) act as an intermediary for mechanical force transfer from soft to hard tissue, as well as an effective interlocking structure to better resist interfacial shear forces. We have developed biphasic constructs that consist of scaffold-free cartilage tissue grown in vitro on, and interdigitated with, porous calcium polyphosphate (CPP) substrates...
August 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28809105/linear-to-%C3%AE-shape-p-o-p-bond-transmutation-in-polyphosphates-with-infinite-po3-%C3%A2-chain
#2
Ying Wang, Lin Li, Shujuan Han, Bing-Hua Lei, Maierhaba Abudoureheman, Zhihua Yang, Shilie Pan
A new metal polyphosphate, α-CsBa2(PO3)5, exhibiting the first example of a linear P-O-P bond angle in a one-dimensional (PO3)∞ chain has been reported. Interestingly, α → β phase transition occurs in CsBa2(PO3)5 along with the P-O-P bonds varying from linear to λ-shape, suggesting that α-CsBa2(PO3)5 with unfavorable linear P-O-P bonds is more stable at ambient temperature.
August 15, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28809038/the-cadmium-tolerance-in-saccharomyces-cerevisiae-depends-on-inorganic-polyphosphate
#3
Ludmila Trilisenko, Ekaterina Kulakovskaya, Tatiana Kulakovskaya
The sensitivity to cadmium (Cd(II)), an important environmental pollutant, was studied in the cells of Saccharomyces cerevisiae strains with genetically altered polyphosphate metabolism. The strains overproducing polyphosphatases PPX1 or PPN1 were more sensitive to Cd(II) than the parent strain. The half maximal inhibitory concentrations were 0.02 and 0.05 mM for the transformants and the parent strain, respectively. Transformant strains cultivated in the presence of Cd(II) show a decrease in the content of short-chained cytosolic acid soluble polyphosphate...
August 15, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28805368/pyrophosphate-recognition-and-sensing-in-water-using-bis-zinc-ii-dipicolylamino-functionalized-peptides
#4
Katrina A Jolliffe
Phosphate oxoanions and phosphorylated biomolecules (such as nucleotides, lipids, and proteins) play key roles in a wide range of biological processes. The ability to selectively detect these ions in the presence of each other has numerous applications in biochemistry and biomedicine. However, receptors and sensors that can discriminate between polyphosphate species with high selectivity and in biologically relevant conditions are rare. In this Account, we show how peptides (both cyclic and linear) can be used to position two zinc(II)dipicolylamine [Zn(II)DPA] binding sites in an appropriate arrangement to provide selective binding of pyrophosphate (PPi) in the presence of other polyphosphate species, including ATP, and in complex media such as cell growth buffer...
August 14, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28803110/dynamics-of-microbial-activities-and-community-structures-in-activated-sludge-under-aerobic-starvation
#5
Wenlong Liu, Yongzhen Peng, Bin Ma, Linna Ma, Fangxu Jia, Xiyao Li
The knowledge of the effect of aerobic starvation on the functional bacterial activities and community structures is important for the recovery of nutrient removal in activated sludge system. Four aerobic starvation processes (3, 7, 14 and 30days) for nitrifiers and polyphosphate accumulating organisms (PAOs) were studied. The results showed that nitrifiers could utilize the released ammonium for growth during the first 7days of the aerobic starvation, and then gradually decayed. In the recovery period, the slower recovery ability of nitrite-oxidizing bacteria (NOB) than ammonium-oxidizing bacteria (AOB) contributed to the nitrite accumulation...
July 25, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28799726/external-hyphae-of-rhizophagus-irregularis-daom-197198-are-less-sensitive-to-low-ph-than-roots-in-arbuscular-mycorrhizae-evidence-from-axenic-culture-system
#6
Ning Wang, Zengwei Feng, Yang Zhou, Honghui Zhu, Qing Yao
The growth of plant roots and arbuscular mycorrhizal fungi (AMF) can be inhibited by low pH; however, it is largely unknown which is more sensitive to low pH. This study aimed to compare the physiological and molecular responses of external hyphae (EH) and roots to low pH in terms of growth, development and functioning. We established AM symbiosis in a two-compartmented system (root compartment, RC; hyphal compartment, HC) using AMF and transformed hairy roots and exposed them to pH 6.5 and/or pH 4.5. The results showed that pH 4...
August 11, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28795780/pseudomonas-aeruginosa-defense-systems-against-microbicidal-oxidants
#7
Bastian Groitl, Jan-Ulrik Dahl, Jeremy W Schroeder, Ursula Jakob
The most abundant oxidants controlling bacterial colonization on mucosal barrier epithelia are hypochlorous acid (HOCl), hypobromous acid (HOBr), and hypothiocyanous acid (HOSCN). All three oxidants are highly antimicrobial but little is known about their relative efficacies, their respective cellular targets, or what specific responses they elicit in bacteria. To address these important questions, we directly tested the individual oxidants on the virulent Pseudomonas aeruginosa strain PA14. We discovered that HOCl and HOBr work almost interchangeably, impacting non-growing bacterial cultures more significantly than actively growing bacteria, and eliciting similar stress responses, including the heat shock response...
August 10, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28792096/the-inositol-pyrophosphate-synthesis-pathway-in-trypanosoma-brucei-is-linked-to-polyphosphate-synthesis-in-acidocalcisomes
#8
Ciro D Cordeiro, Adolfo Saiardi, Roberto Docampo
Inositol pyrophosphates are novel signaling molecules possessing high-energy pyrophosphate bonds and involved in a number of biological functions. Here, we report the correct identification and characterization of the kinases involved in the inositol pyrophosphate biosynthetic pathway in Trypanosoma brucei: inositol polyphosphate multikinase (TbIPMK), inositol pentakisphosphate 2-kinase (TbIP5K) and inositol hexakisphosphate kinase (TbIP6K). TbIP5K and TbIP6K were not identifiable by sequence alone and their activities were validated by enzymatic assays with the recombinant proteins or by their complementation of yeast mutants...
August 9, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28783608/the-effect-of-applying-a-rinse-and-chill-procedure-to-lamb-carcases-immediately-post-death-on-meat-quality
#9
S M Fowler, J M Claus, D L Hopkins
The current study examined the effect of vascular rinsing lamb carcases (Rinse & Chill®) with a commercially available isotonic substrate solution (98.5% water; balance: glucose, polyphosphates, and maltose) at 14°C. Thirty, lamb carcases were randomly allocated to receive either the solution (n=15) 25 min post slaughter on a slaughter line or no solution (n=15). There was no apparent effect on the rate of pH decline due to infusion or muscle shortening of the M. longissimus thoracis et lumborum (LL), but there was a more than 50% reduction in toughness (lower shear force) of the LL due to infusion...
July 22, 2017: Meat Science
https://www.readbyqxmd.com/read/28772825/flame-retardancy-of-carbon-fibre-reinforced-sorbitol-based-bioepoxy-composites-with-phosphorus-containing-additives
#10
Andrea Toldy, Péter Niedermann, Ákos Pomázi, György Marosi, Beáta Szolnoki
Carbon fibre reinforced flame-retarded bioepoxy composites were prepared from commercially available sorbitol polyglycidyl ether (SPE) cured with cycloaliphatic amine hardener. Samples containing 1, 2, and 3% phosphorus (P) were prepared using additive type flame retardants (FRs) resorcinol bis(diphenyl phosphate) (RDP), ammonium polyphosphate (APP), and their combinations. The fire performance of the composites was investigated by limiting oxygen index (LOI), UL-94 tests, and mass loss calorimetry. The effect of FRs on the glass transition temperature, and storage modulus was evaluated by dynamic mechanical analysis (DMA), while the mechanical performance was investigated by tensile, bending, and interlaminar shear measurements, as well as by Charpy impact test...
April 27, 2017: Materials
https://www.readbyqxmd.com/read/28770290/inorganic-polyphosphate-induces-accelerated-tube-formation-of-huvec-endothelial-cells
#11
Werner E G Müller, Maximilian Ackermann, Shunfeng Wang, Meik Neufurth, Rafael Muñoz-Espí, Qingling Feng, Heinz C Schröder, Xiaohong Wang
In this study, the effect of inorganic polyphosphate (polyP) on the initial phase of angiogenesis and vascularization was investigated, applying the HUVEC cell tube formation assay. PolyP is a physiological and high energy phosphate polymer which has been proposed to act as a metabolic fuel in the extracellular space with only a comparably low ATP content. The experiments revealed that polyP accelerates tube formation of human umbilical vein endothelial cells (HUVEC), seeded onto a solidified basement membrane extract matrix which contains polyP-metabolizing alkaline phosphatase (ALP) activity...
August 2, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28766615/rare-earth-doped-fluoride-phosphate-glasses-structural-foundations-of-their-luminescence-properties
#12
Gustavo Galleani, Silvia Helena Santagneli, Younes Messaddeq, Marcos de Oliveira, Hellmut Eckert
We report a detailed structural investigation of a series of fluoride-phosphate glasses with different phosphate/fluoride ratios in the system xSr(PO3)2-(100 - x)[AlF3-CaF2-SrF2-MgF2] with x = 5, 10, 20, 40. Raman and multinuclear solid NMR spectroscopies confirm that the polyphosphate network structure is successively transformed to a structure dominated by Al-O-P linkages with increasing AlF3 content. Average numbers of Al-O-P linkages have been quantified by (27)Al/(31)P NMR double-resonance techniques. The majority of the fluoride species are found in an alkaline earth metal/aluminum rich environment...
August 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28761091/a-novel-pharmacodynamic-assay-to-evaluate-the-effects-of-crystallization-inhibitors-on-calcium-phosphate-crystallization-in-human-plasma
#13
M D Ferrer, M M Pérez, M M Cànaves, J M Buades, C Salcedo, J Perelló
Cardiovascular calcification (CVC) is a progressive complication of chronic kidney disease and a predictor of CV events and mortality. The use of biomarkers to predict CV risk and activities of potential or current treatment drugs in these patients could have a crucial impact on therapeutic approaches. Our aim was to develop a novel assay for measurement of the rate of calcium phosphate crystallization in human plasma and provide a tool to evaluate the effects of crystallization inhibitors. The efficacy of inhibitors was determined by adding inhibitory compounds (polyphosphates, fetuin-A, sodium thiosulfate or citrate) to control samples...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28732682/characterization-and-purification-of-proteins-suitable-for-the-production-of-antibodies-against-ca-liberibacter-asiaticus
#14
Huawei Liu, Sagheer Atta, John S Hartung
The citrus disease huanglongbing (HLB), which is caused by 'Candidatus Liberibacter asiaticus' (CaLas), is one of the most devastating pathogens of citrus, and with no effective method of control, poses a serious threat to citrus production throughout the world. In a previous study we described the production of single chain antibodies against several CaLas proteins that provide the basis for efficient and accurate detection of CaLas in citrus tissues. The isolation of a sufficient amount of purified antigen is a key step in the production of functional antibodies...
July 18, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28731339/colloidal-bound-polyphosphates-and-organic-phosphates-are-bioavailable-a-nutrient-solution-study
#15
Jessica Bollyn, Joran Faes, Andreas Fritzsche, Erik Smolders
Colloidal forms of Fe(III) minerals can be stabilized in solution by coatings of organic or poly-phosphate (P), which reduce the zeta-potential. This opens up a route toward the development of nanoforms of P fertilizers. However, it is unclear if such P forms are bioavailable. To address this question, spinach (Spinacia oleracea) was grown in nutrient solutions, at equal total P, using three different forms of P (orthophosphate = Pi; hexametaphosphate = HMP; myo-inositol hexaphosphate = IHP), free or bound to goethite/ferrihydrite colloids...
August 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28718056/aeration-control-strategies-to-stimulate-simultaneous-nitrification-denitrification-via-nitrite-during-the-formation-of-aerobic-granular-sludge
#16
Thomas Dobbeleers, Jolien D'aes, Solange Miele, Michel Caluwé, Veerle Akkermans, Dominique Daens, Luc Geuens, Jan Dries
In this study, a sequencing batch reactor (SBR), treating synthetic wastewater (COD/N = 5), was operated in two stages. During stage I, an aeration control strategy based on oxygen uptake rate (OUR) was applied, to accomplish nitrogen removal via nitrite >80%. In stage II, the development of aerobic granular sludge (AGS) was examined while two aeration control strategies (OUR and pH slope) maintained the nitrite pathway and optimized the simultaneous nitrification-denitrification (SND) performance. Stimulation of slow-growing organisms, (denitrifying) polyphosphate-accumulating organisms (D)PAO and (denitrifying) glycogen-accumulating organisms (D)GAO leads to full granulation (at day 200, SVI10 = 47...
July 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28712337/overall-effect-of-carbon-production-and-nutrient-release-in-sludge-holding-tank-on-mainstream-biological-nutrient-removal-efficiency
#17
Pouria Jabari, Qiuyan Yuan, Jan A Oleszkiewicz
The objective of this study was to assess the potential of hydrolysis/fermentation of activated sludge in sludge holding tank (SHT) to produce additional carbon for biological nutrient removal (BNR) process. The study was conducted in anaerobic batch tests using the BNR sludge (from a full-scale Westside process) and the mixture of BNR sludge with conventional non-BNR activated sludge (to have higher biodegradable particulate COD (bpCOD) in sludge). At different times during the batch tests the nutrient release, filtered COD (FCOD) production and polyphosphate (poly-P) and aerobic phosphorous uptake of sludge were measured...
July 17, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28705712/hydrolysis-of-diadenosine-polyphosphates-exploration-of-an-additional-role-of-mycobacterium-smegmatis-mutt1
#18
S M Arif, U Varshney, M Vijayan
Diadenosine polyphosphates (ApnA, n=2-6), particularly Ap4A, are involved in several important physiological processes. The substantial sequence identity of the Nudix hydrolase domain (domain 1) of Mycobacterium smegmatis MutT1 (MsMutT1) with a known Ap4A hydrolase suggested that MsMutT1 could also hydrolyse diadenosine polyphosphates. Biochemical experiments yielded results in conformity with this suggestion, with Ap4A as the best among the substrates. ATP is a product in all experiments; small amounts of ADP were also observed in the experiments involving Ap4A and Ap6A...
July 10, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28701714/structural-and-biochemical-characterization-of-apicomplexan-inorganic-pyrophosphatases
#19
Abhishek Jamwal, Manickam Yogavel, Malik Z Abdin, Swatantra K Jain, Amit Sharma
Inorganic pyrophosphatases (PPase) participate in energy cycling and they are essential for growth and survival of organisms. Here we report extensive structural and functional characterization of soluble PPases from the human parasites Plasmodium falciparum (PfPPase) and Toxoplasma gondii (TgPPase). Our results show that PfPPase is a cytosolic enzyme whose gene expression is upregulated during parasite asexual stages. Cambialistic PfPPase actively hydrolyzes linear short chain polyphosphates like PPi, polyP3 and ATP in the presence of Zn(2+)...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28696004/frequent-loss-of-inositol-polyphosphate-5-phosphatase-in-oropharyngeal-squamous-cell-carcinoma
#20
A B Patel, A R Mangold, C M Costello, T H Nagel, M L Smith, R E Hayden, A Sekulic
There is an incomplete understanding in the pathogenesis of oropharyngeal squamous cell carcinoma (OPSCC) and cancer broadly. It is essential to discover new pathways of normal cell-cycle regulation and oncogenesis. We identified that the loss of inositol polyphosphate-5-phosphatase (INPP5A) may play a role in the development and progression of cutaneous squamous cell carcinoma (SCC).(1) Loss of INPP5A has been previously linked to cancer development and progression.(2) This article is protected by copyright...
July 11, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
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