keyword
MENU ▼
Read by QxMD icon Read
search

polyphosphate

keyword
https://www.readbyqxmd.com/read/28718056/aeration-control-strategies-to-stimulate-simultaneous-nitrification-denitrification-via-nitrite-during-the-formation-of-aerobic-granular-sludge
#1
Thomas Dobbeleers, Jolien D'aes, Solange Miele, Michel Caluwé, Veerle Akkermans, Dominique Daens, Luc Geuens, Jan Dries
In this study, a sequencing batch reactor (SBR), treating synthetic wastewater (COD/N = 5), was operated in two stages. During stage I, an aeration control strategy based on oxygen uptake rate (OUR) was applied, to accomplish nitrogen removal via nitrite >80%. In stage II, the development of aerobic granular sludge (AGS) was examined while two aeration control strategies (OUR and pH slope) maintained the nitrite pathway and optimized the simultaneous nitrification-denitrification (SND) performance. Stimulation of slow-growing organisms, (denitrifying) polyphosphate-accumulating organisms (D)PAO and (denitrifying) glycogen-accumulating organisms (D)GAO leads to full granulation (at day 200, SVI10 = 47...
July 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28712337/overall-effect-of-carbon-production-and-nutrient-release-in-sludge-holding-tank-on-mainstream-biological-nutrient-removal-efficiency
#2
Pouria Jabari, Qiuyan Yuan, Jan A Oleszkiewicz
The objective of this study was to assess the potential of hydrolysis/fermentation of activated sludge in sludge holding tank (SHT) to produce additional carbon for biological nutrient removal (BNR) process. The study was conducted in anaerobic batch tests using the BNR sludge (from a full-scale Westside process) and the mixture of BNR sludge with conventional non-BNR activated sludge (to have higher biodegradable particulate COD (bpCOD) in sludge). At different times during the batch tests the nutrient release, filtered COD (FCOD) production and polyphosphate (poly-P) and aerobic phosphorous uptake of sludge were measured...
July 17, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28705712/hydrolysis-of-diadenosine-polyphosphates-exploration-of-an-additional-role-of-mycobacterium-smegmatis-mutt1
#3
S M Arif, U Varshney, M Vijayan
Diadenosine polyphosphates (ApnA, n=2-6), particularly Ap4A, are involved in several important physiological processes. The substantial sequence identity of the Nudix hydrolase domain (domain 1) of Mycobacterium smegmatis MutT1 (MsMutT1) with a known Ap4A hydrolase suggested that MsMutT1 could also hydrolyse diadenosine polyphosphates. Biochemical experiments yielded results in conformity with this suggestion, with Ap4A as the best among the substrates. ATP is a product in all experiments; small amounts of ADP were also observed in the experiments involving Ap4A and Ap6A...
July 10, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28701714/structural-and-biochemical-characterization-of-apicomplexan-inorganic-pyrophosphatases
#4
Abhishek Jamwal, Manickam Yogavel, Malik Z Abdin, Swatantra K Jain, Amit Sharma
Inorganic pyrophosphatases (PPase) participate in energy cycling and they are essential for growth and survival of organisms. Here we report extensive structural and functional characterization of soluble PPases from the human parasites Plasmodium falciparum (PfPPase) and Toxoplasma gondii (TgPPase). Our results show that PfPPase is a cytosolic enzyme whose gene expression is upregulated during parasite asexual stages. Cambialistic PfPPase actively hydrolyzes linear short chain polyphosphates like PPi, polyP3 and ATP in the presence of Zn(2+)...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28696004/frequent-loss-of-inositol-polyphosphate-5-phosphatase-in-oropharyngeal-squamous-cell-carcinoma
#5
A B Patel, A R Mangold, C M Costello, T H Nagel, M L Smith, R E Hayden, A Sekulic
There is an incomplete understanding in the pathogenesis of oropharyngeal squamous cell carcinoma (OPSCC) and cancer broadly. It is essential to discover new pathways of normal cell-cycle regulation and oncogenesis. We identified that the loss of inositol polyphosphate-5-phosphatase (INPP5A) may play a role in the development and progression of cutaneous squamous cell carcinoma (SCC).(1) Loss of INPP5A has been previously linked to cancer development and progression.(2) This article is protected by copyright...
July 11, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28690145/salivary-gland-transcripts-of-the-kissing-bug-panstrongylus-chinai-a-vector-of-chagas-disease
#6
Hirotomo Kato, Ryan C Jochim, Eduardo A Gomez, Shunsuke Tsunekawa, Jesus G Valenzuela, Yoshihisa Hashiguchi
The saliva of hematophagous arthropods injected during blood feeding contains potent pharmacologically active components to counteract the host hemostatic and inflammatory systems. In the present study, dominant salivary gland transcripts of Panstrongylus chinai, a vector of Chagas disease, were analyzed by sequencing randomly selected clones of the salivary gland cDNA library. This analysis showed that 56.5% of the isolated transcripts coded for putative secreted proteins, of which 73.7% coded for proteins belonging to the lipocalin family...
July 6, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28687622/polyphosphate-as-donor-of-high-energy-phosphate-for-the-synthesis-of-adp-and-atp
#7
Werner E G Müller, Shunfeng Wang, Meik Neufurth, Maria Kokkinopoulou, Qingling Feng, Heinz C Schröder, Xiaohong Wang
We studied the potential role of inorganic polyphosphate (polyP) as an energy source for ADP and ATP formation in the extracellular space. Using SaOS-2 we show that matrix vesicles are released into the extracellular space after incubation with polyP. These vesicles contain both the alkaline phosphatase (ALP) and the adenylate kinase (AK). Both enzymes translocate to the cell membrane in response to polyP. To distinguish the process(es) (i) AMP and ADP formation during ALP hydrolysis, from the (ii) ATP generation via the AK reaction, inhibition studies with the AK inhibitor A(5')P5(5')A were performed...
July 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28678862/pseudomonas-aeruginosa-cells-attached-to-a-surface-display-a-typical-proteome-early-as-20-minutes-of-incubation
#8
Marc Crouzet, Stéphane Claverol, Anne-Marie Lomenech, Caroline Le Sénéchal, Patricia Costaglioli, Christophe Barthe, Bertrand Garbay, Marc Bonneu, Sébastien Vilain
Biofilms are present in all environments and often result in negative effects due to properties of the biofilm lifestyle and especially antibiotics resistance. Biofilms are associated with chronic infections. Controlling bacterial attachment, the first step of biofilm formation, is crucial for fighting against biofilm and subsequently preventing the persistence of infection. Thus deciphering the underlying molecular mechanisms involved in attachment could allow discovering molecular targets from it would be possible to develop inhibitors against bacterial colonization and potentiate antibiotherapy...
2017: PloS One
https://www.readbyqxmd.com/read/28678391/interferon-lambda1-il-29-and-inorganic-polyphosphate-are-novel-regulators-of-neutrophil-driven-thromboinflammation
#9
Akrivi Chrysanthopoulou, Konstantinos Kambas, Dimitrios Stakos, Ioannis Mitroulis, Alexandros Mitsios, Veroniki Vidali, Iliana Angelidou, Magdalena Bochenek, Stella Arelaki, Athanasios Arampatzioglou, Ioanna-Evdokia Galani, Panagiotis Skendros, Elias A Couladouros, Stavros Konstantinides, Evangelos Andreakos, Katrin Schäfer, Konstantinos Ritis
Neutrophils and neutrophil-released meshwork structures termed neutrophil extracellular traps (NETs) are major mediators of thromboinflammation and emerging targets for therapy, yet the mechanisms and pathways that control the role of neutrophils in thromboinflammation remain poorly understood. Here, we explored the role of IFN-λ1/IL-29, a major antiviral cytokine recently shown to suppress the neutrophil migratory capacity, in prothrombotic and proNETotic functions of neutrophils. In an ex vivo human experimental setting of acute ST-segment elevation myocardial infarction (STEMI), we show that IFN-λ1/IL-29 hinders NET release and diminishes the amount of cytoplasmic TF in neutrophils...
July 5, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28677086/preparation-and-performance-of-biofouling-resistant-pan-chitosan-hollow-fiber-membranes
#10
D Shanthana Lakshmi, Santlal Jaiswar, Mayank Saxena, Franco Tasselli, Hiren D Raval
The preparation of polyacrylonitrile (PAN) hollow fiber (HF) membranes has been carried out by dry-jet wet spinning. PAN HF membranes were coated with chitosan biopolymers 2 wt% by dip coating and further crosslinked by chemical reagents (Tri sodium polyphosphate). PAN HF (Virgin) and PAN/chitosan coated membrane were characterized by SEM and tested for water flux. Proteins Pepsin, Albumin, and Clay of 1000 ppm concentration were tested for separation efficiency. In addition, bacterial species Escherichia coli and Bacillus subtilis were tested for fouling control efficiency and found out that PAN/chitosan membranes were quite superior to virgin PAN fibers...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28675869/flame-retardant-and-hydrophobic-coatings-on-cotton-fabrics-via-sol-gel-and-self-assembly-techniques
#11
Dongqiao Zhang, Brandon L Williams, Saral B Shrestha, Zain Nasir, Elaina M Becher, Benjamin J Lofink, Victor H Santos, Harsh Patel, Xiaohong Peng, Luyi Sun
Nanocoatings consisting of ammonium polyphosphate (APP), sodium montmorillonite (MMT), and vinyltrimethoxysilane (VTMS) were prepared via self-assembly and in situ sol-gel techniques and applied onto cotton fabrics to achieve both flame retardancy and hydrophobicity. The impacts of APP concentration on the hydrophobicity and fire resistance of the coated fabrics were investigated. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) characterization results verified the hydrolysis-condensation reaction of VTMS and the formation of Si-O-Si network structure...
June 27, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28669993/the-5-phosphatase-ocrl-in-lowe-syndrome-and-dent-disease-2
#12
REVIEW
Maria Antonietta De Matteis, Leopoldo Staiano, Francesco Emma, Olivier Devuyst
Lowe syndrome is an X-linked disease that is characterized by congenital cataracts, central hypotonia, intellectual disability and renal Fanconi syndrome. The disease is caused by mutations in OCRL, which encodes an inositol polyphosphate 5-phosphatase (OCRL) that acts on phosphoinositides - quantitatively minor constituents of cell membranes that are nonetheless pivotal regulators of intracellular trafficking. In this Review we summarize the considerable progress made over the past decade in understanding the cellular roles of OCRL in regulating phosphoinositide balance along the endolysosomal pathway, a fundamental system for the reabsorption of proteins and solutes by proximal tubular cells...
August 2017: Nature Reviews. Nephrology
https://www.readbyqxmd.com/read/28668748/effect-of-ciprofloxacin-on-biological-nitrogen-and-phosphorus-removal-from-wastewater
#13
Kaixin Yi, Dongbo Wang, QiYang, Xiaoming Li, Hongbo Chen, Jian Sun, Hongxue An, Liqun Wang, Yongchao Deng, Jun Liu, Guangming Zeng
In this work, both short-term and long-term experiments were therefore conducted to assess the effects of ciprofloxacin (0.2 and 2mg·L(-1)) on wastewater nutrient removal. The results showed that both levels of ciprofloxacin had no acute and chronic adverse effects on the surface integrity and viability of activated sludge. Short-term exposure to all the ciprofloxacin levels induced negligible influences on wastewater nutrient removal. However, the prolonged exposure to ciprofloxacin decreased total phosphorus and nitrogen removal efficiencies from 96...
June 29, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28666149/phosphorus-removal-performance-and-biological-dephosphorization-process-in-treating-reclaimed-water-by-integrated-vertical-flow-constructed-wetlands-ivcws
#14
Lu Du, Qianru Chen, Panpan Liu, Xia Zhang, Huihui Wang, Qiaohong Zhou, Dong Xu, Zhenbin Wu
Phosphorous removal in adsorption had been extensively researched; however, the biological dephosphorization process and optimum operating parameters have not been discussed or quantified in Integrated Vertical-flow Constructed Wetlands (IVCWs). In this study, IVCWs planted with different plants were employed to evaluate total phosphorus (TP) treatment performance under different hydraulic retention times (HRTs), in summer and autumn. The results showed that the systems planted with Canna generalis showed the highest TP removal efficiency (77%) under a three-day HRT in autumn...
June 19, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28660385/genetic-improvement-of-magnetospirillum-gryphiswaldense-for-enhanced-biological-removal-of-phosphate
#15
Yanping Zhou, Wiktor Lisowski, Yan Zhou, Ng Wun Jern, Kama Huang, Eileen Fong
OBJECTIVES: To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase. RESULTS: Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained...
June 28, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28657292/plasma-functionalized-polytetrafluoroethylene-nanoparticles-for-improved-wear-in-lubricated-contact
#16
Vinay Sharma, Richard Timmons, Ali Erdemir, Pranesh B Aswath
Plasma-functionalized polytetrafluoroethylene (PTFE) nanoparticles were employed to evaluate their utility in improving the lubrication property of a group III mineral oil with a significantly low amount of zinc dialkyl dithiophosphate (ZDDP). The particles were coated with two consecutive films; the initial coating contained silica to enhance amorphous glassy tribofilm formation, followed by a methacrylate film to protect the silica coating and enhance dispersibility in the oil. The functionalized nanoparticles were evaluated for their tribological performance using a high-frequency reciprocating rig, in a cylinder-on-flat configuration...
July 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28653606/mir-181a-5p-promotes-proliferation-and-invasion-and-inhibits-apoptosis-of-cervical-cancer-cells-via-regulating-inositol-polyphosphate-5-phosphatase-a-inpp5a
#17
Meng Yang, Xu Zhai, Tingting Ge, Chang Yang, Ge Lou
Expression of miR-181a-5p associates with the proliferation and progression of cancer cells via its targets. This studywas designed to investigate the effect of miR-181a-5p and its target inositol polyphosphate-5-phosphatase A (INPP5A) on progression of cervical cancers. Upregulation of miR-181a-5p was revealed in cervical cancer cell lines HeLa and SiHa in comparison with End1/E6E7 (p < 0.001). The inhibition and upregulation of miR-181a-5p in cervical cancer cell lines significantly reduced or increased cell proliferation and invasion capacity, accompanied with enhanced or reduced apoptosis (p < 0...
June 23, 2017: Oncology Research
https://www.readbyqxmd.com/read/28651489/inorganic-polyphosphate-enhances-radio-sensitivity-in-a-human-non-small-cell-lung-cancer-cell-line-h1299
#18
Kaori Tsutsumi, Yusuke Matsuya, Tomoki Sugahara, Manami Tamura, Satoshi Sawada, Sagiri Fukura, Hisashi Nakano, Hiroyuki Date
Inorganic polyphosphate is a linear polymer containing tens to hundreds of orthophosphate residues linked by high-energy phosphoanhydride bonds. Polyphosphate has been recognized as a potent anti-metastasis reagent. However, the molecular mechanism underlying polyphosphate action on cancer cells is poorly understood. In this study, we investigated the involvement of polyphosphate in radio-sensitivity using a human non-small cell lung cancer cell line, H1299. We found that polyphosphate treatment decreases cellular adenosine triphosphate levels, suggesting a disruption of energy metabolism...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28650469/a-compartmentalized-phosphoinositide-signaling-axis-at-cilia-is-regulated-by-inpp5e-to-maintain-cilia-and-promote-sonic-hedgehog-medulloblastoma
#19
S E Conduit, V Ramaswamy, M Remke, D N Watkins, B J Wainwright, M D Taylor, C A Mitchell, J M Dyson
Sonic Hedgehog (SHH) signaling at primary cilia drives the proliferation and progression of a subset of medulloblastomas, the most common malignant paediatric brain tumor. Severe side effects associated with conventional treatments and resistance to targeted therapies has led to the need for new strategies. SHH signaling is dependent on primary cilia for signal transduction suggesting the potential for cilia destabilizing mechanisms as a therapeutic target. INPP5E is an inositol polyphosphate 5-phosphatase that hydrolyses PtdIns(4,5)P2 and more potently, the phosphoinositide (PI) 3-kinase product PtdIns(3,4,5)P3...
June 26, 2017: Oncogene
https://www.readbyqxmd.com/read/28648175/enzymatic-phosphorylation-of-mannose-by-glucomannokinase-from-mycobacterium-phlei-using-inorganic-polyphosphate
#20
Shafaq Parveen, Biqiang Chen, Luo Liu, Tianwei Tan
Mannose-6-phosphate is an important phosphor-sugar, which is involved in many physiological functions and it is used to treat many diseases. Its production is however expensive since it requires costly substrate ATP as phosphorylation agent. This study has focused upon the direct synthesis of M6P by glucomannokinase using inorganic polyphosphate without involvement of ATP. The gene cloned for glucomannokinase has been sequenced from Mycobacterium phlei and it is transformed into Escherichia coli for expression...
September 2017: Enzyme and Microbial Technology
keyword
keyword
108392
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"