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https://www.readbyqxmd.com/read/28105330/genetic-markers-associated-with-resistance-to-beta-lactam-and-quinolone-antimicrobials-in-non-typhoidal-salmonella-isolates-from-humans-and-animals-in-central-ethiopia
#1
Tadesse Eguale, Josephine Birungi, Daniel Asrat, Moses N Njahira, Joyce Njuguna, Wondwossen A Gebreyes, John S Gunn, Appolinaire Djikeng, Ephrem Engidawork
BACKGROUND: Beta-lactam and quinolone antimicrobials are commonly used for treatment of infections caused by non-typhoidal Salmonella (NTS) and other pathogens. Resistance to these classes of antimicrobials has increased significantly in the recent years. However, little is known on the genetic basis of resistance to these drugs in Salmonella isolates from Ethiopia. METHODS: Salmonella isolates with reduced susceptibility to beta-lactams (n = 43) were tested for genes encoding for beta-lactamase enzymes, and those resistant to quinolones (n = 29) for mutations in the quinolone resistance determining region (QRDR) as well as plasmid mediated quinolone resistance (PMQR) genes using PCR and sequencing...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28103321/derivative-of-extremophilic-50s-ribosomal-protein-l35ae-as-an-alternative-protein-scaffold
#2
Anna V Lomonosova, Andrei B Ulitin, Alexei S Kazakov, Tajib A Mirzabekov, Eugene A Permyakov, Sergei E Permyakov
Small antibody mimetics, or alternative binding proteins (ABPs), extend and complement antibody functionality with numerous applications in research, diagnostics and therapeutics. Given the superiority of ABPs, the last two decades have witnessed development of dozens of alternative protein scaffolds (APSs) for the design of ABPs. Proteins from extremophiles with their high structural stability are especially favorable for APS design. Here, a 10X mutant of the 50S ribosomal protein L35Ae from hyperthermophilic archaea Pyrococcus horikoshii has been probed as an APS...
2017: PloS One
https://www.readbyqxmd.com/read/28102514/carbapenemase-producing-klebsiella-pneumoniae-bloodstream-infection-in-critically-ill-patients-risk-factors-and-predictors-of-mortality
#3
M Papadimitriou-Olivgeris, F Fligou, C Bartzavali, A Zotou, A Spyropoulou, K Koutsileou, S Vamvakopoulou, N Sioulas, V Karamouzos, E D Anastassiou, I Spiliopoulou, M Christofidou, M Marangos
A significant increase in carbapenemase-producing Klebsiella pneumoniae (CP-Kp) bacteraemias has been observed worldwide. The objective of the present work was to study the risk factors and predictors of mortality of CP-Kp bacteraemias among critically ill patients. During a 4-year period (2012-3015), a matched 1:2 case-control study was conducted. Klebsiella pneumoniae was identified by Vitek 2 technology. Antibiotic susceptibility was performed by the agar disc diffusion method and Etest. The presence of the bla KPC, bla VIM and bla NDM genes was confirmed by polymerase chain reaction (PCR)...
January 19, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28095119/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-strains-is-associated-with-worse-outcome-in-patients-hospitalized-in-the-pediatric-oncology-unit-of-beni-messous-hospital-in-algiers-algeria
#4
Chafiaa Medboua-Benbalagh, Abdelaziz Touati, Rachida Kermas, Alima Gharout-Sait, Lucien Brasme, Halima Mezhoud, Djamila Touati, Thomas Guillard, Christophe de Champs
OBJECTIVES: The current study aimed to investigate extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) fecal carriage in children with different cancers admitted in the pediatric oncology unit of Beni-Messous Hospital (Algiers, Algeria). MATERIALS AND METHODS: Rectal swabs from children with cancer were sampled from February 2012 to May 2013 within 48 hours following their admission. After species identification and detection of ESBL production by double-disk synergy test (DD test), antibiotic susceptibility was determined by the standard disk diffusion method...
January 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28094489/the-sex-specific-internal-and-external-demands-imposed-on-players-during-ultimate-frisbee-game-play
#5
Maria C Madueno, Crystal O Kean, Aaron T Scanlan
BACKGROUND: Despite the growing popularity of Ultimate Frisbee (UF) across sexes, the game demands imposed on players have been predominately examined in males. This study aimed to compare the internal and external demands of UF game-play in males and females. METHODS: Male (N = 10) and female (N = 10) recreational UF players competed in separate sex-specific, indoor UF games. Internal responses (blood lactate concentration ([BLa-]), rating of perceived exertion (RPE), and heart rate (HR)) and external responses (direction-specific and total relative (·min-1) PlayerLoadTM (PL), and Estimated Equivalent Distance (EED)) were measured...
January 17, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28092608/white-and-red-blood-cell-picture-in-rabbits-experimentally-infected-with-strains-of-the-rabbit-haemorrhagic-disease-rhd-virus-without-or-with-variable-haemagglutination-capacity
#6
P Niedźwiedzka-Rystwej, B Tokarz-Deptuła, W Deptuła
The aim of the study was to establish if haemagglutination of rabbit haemorrhagic disease virus (RHDV) affects haematological picture of peripheral blood in rabbits and the pathogenicity of the virus. The study analyzed white and red blood cell picture in rabbits experimentally infected with two non-haemagglutinating (HA-) RHDV strains (Frankfurt and Asturias) and one strain with variable haemagglutination capacity (HA+/-) (Hagenow). Studies with HA- and HA +/- are rare and relate only to 4 HA- strains (2 RHDV: BLA and Rainham; 2 RHDVa: Pv97 and 9905) and 1 HA+/- RHDV strain: ŻD, where less changes in haematological indices and less pathogenicity were observed...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28081348/the-effect-of-basolateral-amygdala-nucleus-lesion-on-memory-under-acute-mid-and-chronic-stress-in-male-rats
#7
Hoda Ranjbar, Maryam Radahmadi, Hojjatallah Alaei, Parham Reisi, Sara Karimi
BACKGROUND/AIM: The basolateral amygdala (BLA) modulates memory for emotional events and is involved in both stress and memory. This study investigated different durations of stress and the role of BLA on serum corticosterone level and spatial and cognitive memory. MATERIALS AND METHODS: Different durations of stress (acute, mid, and chronic stress), with and without BLA lesion were induced in rats by 6 h/day restraint stress for 1, 7, and 21 days. Memory functions were evaluated by novel object recognition (NOR) and object location test (OLT)...
December 20, 2016: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28078492/first-report-of-bla-ctx-m-15-type-esbl-producing-klebsiella-pneumoniae-in-wild-migratory-birds-in-pakistan
#8
Shahbaz Raza, Mashkoor Mohsin, Waqas Ahmed Madni, Fatima Sarwar, Muhammad Saqib, Bilal Aslam
We investigated wild migratory birds faecal swabs for extended-spectrum β-lactamases-producing Klebsiella pneumoniae (ESBL-K. pneumoniae) from wetland habitats in Pakistan. ESBL-K. pneumoniae were analysed for MDR phenotype, ESBL genotype and genetic diversity. A total of 13 (8.6%) ESBL-K. pneumoniae were recovered. Of these, 8 (61%) isolates were MDR. DNA sequencing confirmed bla CTX-M-15 as the dominant ESBL genotype. BOX-PCR fingerprints showed most of the isolates are unrelated. This study is the first to report the wildlife contamination of CTX-M-15-producing K...
January 11, 2017: EcoHealth
https://www.readbyqxmd.com/read/28076352/high-intensity-interval-training-elicits-higher-enjoyment-than-moderate-intensity-continuous-exercise
#9
Jacob S Thum, Gregory Parsons, Taylor Whittle, Todd A Astorino
: Exercise adherence is affected by factors including perceptions of enjoyment, time availability, and intrinsic motivation. Approximately 50% of individuals withdraw from an exercise program within the first 6 mo of initiation, citing lack of time as a main influence. Time efficient exercise such as high intensity interval training (HIIT) may provide an alternative to moderate intensity continuous exercise (MICT) to elicit substantial health benefits. This study examined differences in enjoyment, affect, and perceived exertion between MICT and HIIT...
2017: PloS One
https://www.readbyqxmd.com/read/28067350/enzyme-responsive-reporter-molecules-for-selective-localization-and-fluorescence-imaging-of-pathogenic-biofilms
#10
Junxin Aw, Frances Widjaja, Yichen Ding, Jing Mu, Yang Liang, Bengang Xing
Pathogenic bacteria and their biofilm formation are responsible for a broad spectrum of microbial infections. A novel enzyme-responsive reporter molecule (ERM-1), which can specifically recognize AmpC β-lactamase (Bla) in drug resistant bacteria, has been developed to enable the selective localization of biofilms.
January 9, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28065891/genetic-and-biochemical-characterization-of-hmb-1-a-novel-subclass-b1-metallo-%C3%AE-lactamase-found-in-a-pseudomonas-aeruginosa-clinical-isolate
#11
Niels Pfennigwerth, Felix Lange, Cristina Belmar Campos, Moritz Hentschke, Sören G Gatermann, Martin Kaase
OBJECTIVES: To characterize a novel subclass B1 metallo-β-lactamase (MBL) found in an MDR Pseudomonas aeruginosa clinical isolate. METHODS: The isolate P. aeruginosa NRZ-03096 was recovered in 2012 from an anal swab from a patient hospitalized in Northern Germany and showed high MICs of carbapenems. MBL production was analysed by several phenotypic tests. Genetic characterization of the novel bla gene and MLST was performed by WGS. The novel bla gene was expressed in Escherichia coli TOP10 and the enzyme was subjected to biochemical characterization to determine the kinetic parameters Km and kcat RESULTS: P...
January 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28063155/effects-of-electrical-lesion-of-basolateral-amygdala-nucleus-on-rat-anxiety-like-behavior-under-acute-sub-chronic-and-chronic-stresses
#12
Hoda Ranjbar, Maryam Radahmadi, Parham Reisi, Hojjatallah Alaei
Stress contributes, as a risk factor, to such psychological disorders as anxiety. The effects of electrical lesions in the basolateral amygdala nucleus (BLA) were investigated on the locomotor activity and anxiety-like behavior in different stress durations. For this purpose, rats were randomly allocated to control, sham, and experimental groups, the latter including groups with and without BLA nucleus subjected to acute, sub-chronic, and chronic stress conditions for 1, 7, and 21 days, respectively, applied 6 hours/day...
January 7, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28063000/evaluation-of-loop-mediated-isothermal-amplification-for-the-rapid-identification-of-bacteria-and-resistance-determinants-in-positive-blood-cultures
#13
J Rödel, J A Bohnert, S Stoll, L Wassill, B Edel, M Karrasch, B Löffler, W Pfister
The use of molecular assays to rapidly identify pathogens and resistance genes directly from positive blood cultures (BCs) contribute to shortening the time required for the diagnosis of bloodstream infections. In this work, loop-mediated isothermal amplification (LAMP) assays have been examined for their potential use in BC diagnosis. Three different assays were applied. The commercially available eazyplex® MRSA test detects Staphylococcus aureus, S. epidermidis, mecA, and mecC. Two in-house assays [Gram-positive (GP) and Gram-negative (GN)] have been developed for the detection of streptococci, enterococci, vanA, vanB, Pseudomonas spp...
January 6, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28056839/molecular-epidemiology-of-bla-oxa-23-producing-carbapenem-resistant-acinetobacter-baumannii-in-a-single-institution-over-a-65-month-period-in-north-china
#14
Nian-Zhi Ning, Xiong Liu, Chun-Mei Bao, Su-Ming Chen, En-Bo Cui, Ju-Ling Zhang, Jie Huang, Fang-Hong Chen, Tao Li, Fen Qu, Hui Wang
BACKGROUND: Carbapenem-resistant Acinetobacter baumannii poses a significant threat to hospitalized patients, as few therapeutic options remain. Thus, we investigated the molecular epidemiology and mechanism of resistance of carbapenem-resistant A.baumannii isolates in Beijing, China. METHODS: Carbapenem-resistant A.baumannii isolates (n = 101) obtained between June 2009 and November 2014 were used. Multilocus sequence typing (MLST) and PCR assays for class C and D β-lactamase were performed on all isolates...
January 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28039104/rectal-carriage-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-enterobacteriaceae-among-hospitalised-neonates-in-a-neonatal-intensive-care-unit-in-fez-morocco
#15
Btissam Arhoune, Bouchra Oumokhtar, Fouzia Hmami, Abouddihaj Barguigua, Mohammed Timinouni, Samira El Fakir, Fouzia Chami, Abdelhak Bouharrou
OBJECTIVES: The aim of this study was to investigate the faecal carriage and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBLE) isolated from rectal samples of neonates hospitalised in a neonatal intensive care unit (NICU) of a university hospital in Fez, Morocco. METHODS: From February-July 2013, all neonates hospitalised in the NICU were screened for ESBLE carriage at discharge. ESBLs were identified by double-disk synergy test, PCR and DNA sequencing analysis...
December 27, 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28035181/functional-connectivity-of-basolateral-amygdala-neurons-carrying-orexin-receptors-and-melanin-concentrating-hormone-receptors-in-regulating-sociability-and-mood-related-behaviors
#16
Tae-Kyung Kim, Pyung-Lim Han
Chronic stress induces changes in neuronal functions in specific brain regions regulating sociability and mood-related behaviors. Recently we reported that stress-induced persistent upregulation of the neuropeptides orexin and melanin-concentrating hormone (MCH) in the basolateral amygdala (BLA) and the resulting activation of orexin receptors or MCH receptors within the BLA produced deficits in sociability and mood-related behaviors. In the present study, we investigated the neural targets that were innervated by BLA neurons containing orexin receptors or MCH receptors...
December 2016: Experimental Neurobiology
https://www.readbyqxmd.com/read/28034786/bidirectional-modulation-of-taste-aversion-extinction-by-insular-cortex-ltp-and-ltd
#17
Luis F Rodríguez-Durán, Araceli Martínez-Moreno, Martha L Escobar
The history of activity of a given neuron has been proposed to bidirectionally influence its future response to synaptic inputs. In particular, induction of synaptic plasticity expressions such as long-term potentiation (LTP) and long-term depression (LTD) modifies the performance of several behavioral tasks. Our previous studies in the insular cortex (IC), a neocortical region that has been related to acquisition and retention of conditioned taste aversion (CTA), have demonstrated that induction of LTP in the basolateral amygdaloid nucleus (Bla)-IC pathway before CTA training enhances the retention of this task...
December 27, 2016: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/28030757/altered-resting-state-functional-connectivity-of-fear-and-reward-circuitry-in-comorbid-ptsd-and-major-depression
#18
Xi Zhu, Liat Helpman, Santiago Papini, Franklin Schneier, John C Markowitz, Page E Van Meter, Martin A Lindquist, Tor D Wager, Yuval Neria
BACKGROUND: Individuals with comorbid posttraumatic stress disorder and major depressive disorder (PTSD-MDD) often exhibit greater functional impairment and poorer treatment response than individuals with PTSD alone. Research has not determined whether PTSD-MDD is associated with different network connectivity abnormalities than PTSD alone. METHODS: We used functional magnetic resonance imaging (fMRI) to measure resting state functional connectivity (rs-FC) patterns of brain regions involved in fear and reward processing in three groups: patients with PTSD-alone (n = 27), PTSD-MDD (n = 21), and trauma-exposed healthy controls (TEHCs, n = 34)...
December 28, 2016: Depression and Anxiety
https://www.readbyqxmd.com/read/28028604/molecular-mechanisms-of-group-i-metabotropic-glutamate-receptor-mediated-ltp-and-ltd-in-basolateral-amygdala-in-vitro
#19
A Chen, W W Hu, X L Jiang, M Potegal, H Li
The roles of group I metabotropic glutamate receptors, metabotropic glutamate receptor 1 (mGluR1) and mGluR5, in regulating synaptic plasticity and metaplasticity in the basolateral amygdala (BLA) remain unclear. The present study examined mGluR1- and mGluR5-mediated synaptic plasticity in the BLA and their respective signaling mechanisms. Bath application of the group I mGluR agonist, 3,5-dihydroxyphenylglycine (DHPG) (20 μM), directly suppressed basal fEPSPs (84.5 ± 6.3% of the baseline). The suppressive effect persisted for at least 30 min after washout; it was abolished by the mGluR1 antagonist 7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxylate ethyl ester (CPCCOEt) but was unaffected by the mGluR5 antagonist 2-methyl-6- (phenylethynyl)-pyridine (MPEP)...
December 28, 2016: Psychopharmacology
https://www.readbyqxmd.com/read/28028198/sex-dependent-regulation-of-aromatase-mediated-synaptic-plasticity-in-the-basolateral-amygdala
#20
Roland A Bender, Lepu Zhou, Ricardo Vierk, Nicola Brandt, Alexander Keller, Christine E Gee, Michael K E Schäfer, Gabriele M Rune
: The basolateral amygdala (BLA) integrates sensory input from cortical and subcortical regions, a function that requires marked synaptic plasticity. Here we provide evidence that cytochrome P450 aromatase (AROM), the enzyme converting testosterone to 17β-estradiol (E2), contributes to the regulation of this plasticity in a sex-specific manner. We show that AROM is expressed in the BLA, particularly in the basolateral nucleus (BL), in male and female rodents. Systemic administration of the AROM-inhibitor letrozole reduced spine synapse density in the BL of adult female mice, but not in the BL of male mice...
December 27, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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