keyword
MENU ▼
Read by QxMD icon Read
search

Bla

keyword
https://www.readbyqxmd.com/read/27913108/chicken-line-dependent-mortality-after-experimental-infection-with-three-type-iixiii-recombinant-toxoplasma-gondii-clones
#1
G Schares, D C Herrmann, P Maksimov, B Matzkeit, F J Conraths, G Moré, R Preisinger, S Weigend
Three genetically different clones of Toxoplasma gondii, also different in mouse virulence, were studied by experimental infection in chickens. For the experiments, four chicken lines were used, which differed in phylogenetic origin and performance level: two white egg layer lines, one with high laying performance (WLA), one with low (R11) and two brown layer lines, also displaying high (BLA) and low (L68) egg number. Chickens were intraperitoneally infected with three different T. gondii isolates representing type IIxIII recombinant clones, i...
November 29, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27905406/differential-roles-for-l-type-calcium-channel-subtypes-in-alcohol-dependence
#2
Stefanie Uhrig, David Vandael, Andrea Marcantoni, Nina Dedic, Ainhoa Bilbao, Miriam A Vogt, Natalie Hirth, Laura Broccoli, Rick E Bernardi, Kai Schönig, Peter Gass, Dusan Bartsch, Rainer Spanagel, Jan M Deussing, Wolfgang H Sommer, Emilio Carbone, Anita C Hansson
It has previously been shown that the inhibition of L-type calcium channels (LTCCs) decreases alcohol consumption, although the contribution of the central LTCC subtypes Cav1.2 and Cav1.3 remains unknown. Here, we determined changes in Cav1.2 (Cacna1c) and Cav1.3 (Cacna1d) mRNA and protein expression in alcohol dependent rats during protracted abstinence and naïve controls using in situ hybridization and Western Blot analysis. Functional validation was obtained by electrophysiological recordings of calcium currents in dissociated hippocampal pyramidal neurons...
December 1, 2016: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/27899281/sex-differences-in-responses-of-the-basolateral-central-amygdala-circuit-to-alcohol-corticosterone-and-their-interaction
#3
Marian L Logrip, Christopher Oleata, Marisa Roberto
Alcohol use disorders are chronically relapsing conditions that pose significant health challenges for our society. Stress is a prevalent trigger of relapse, particularly for women, yet the mechanisms by which alcohol and stress interact, and how this differs between males and females, remain poorly understood. The glutamatergic circuit connecting the basolateral (BLA) and central (CeA) nuclei of the amygdala is a likely locus for such adaptations, yet the impact of alcohol, corticosterone and their interaction on this circuit has been understudied...
November 26, 2016: Neuropharmacology
https://www.readbyqxmd.com/read/27891098/deceptive-manipulation-of-competitive-starting-strategies-influences-subsequent-pacing-physiological-status-and-perceptual-responses-during-cycling-time-trials
#4
Emily L Williams, Hollie S Jones, S Andy Sparks, David C Marchant, Adrian W Midgley, Craig A Bridge, Lars R McNaughton
Little is currently known regarding competitor influence on pacing at the start of an event and in particular the subsequent effect on the remaining distance. The purpose of the present study was to investigate the influence of starting pace on the physiological and psychological responses during cycling time trials (TT) utilizing an innovative approach allowing pace to be accurately and dynamically replicated, as well as deceptively manipulated. Ten competitive male cyclists completed five 16.1 km TT, two baseline trials performed alone (BLs), and three with a simulated, dynamic avatar of which they were to match the pace of for the initial 4 km...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27887603/genetic-elements-associated-with-antimicrobial-resistance-among-avian-pathogenic-escherichia-coli
#5
Amal Awad, Nagah Arafat, Mohamed Elhadidy
BACKGROUND: Avian-pathogenic Escherichia coli (APEC) are pathogenic strains of E. coli that are responsible for one of the most predominant bacterial disease affecting poultry worldwide called avian colibacillosis. This study describes the genetic determinants implicated in antimicrobial resistance among APEC isolated from different broiler farms in Egypt. METHODS: A total of 116 APEC were investigated by serotyping, antimicrobial resistance patterns to 10 antimicrobials, and the genetic mechanisms underlying the antimicrobial-resistant phenotypes...
November 25, 2016: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/27882043/why-cannot-a-%C3%AE-lactamase-gene-be-detected-using-an-efficient-molecular-diagnostic-method
#6
Kwang Seung Park, Jung Hun Lee, Moonhee Park, Asad Mustafa Karim, Sang Hee Lee
OBJECTIVE: Fast detection of β-lactamase (bla) genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited bla gene types, these methods have significant limitations, such as their failure to detect almost all clinically available bla genes. We have evaluated a further refinement of our fast and accurate molecular method, developed to overcome these limitations, using clinical isolates. METHODS: We have recently developed the efficient large-scale bla detection method (large-scaleblaFinder) that can detect bla gene types including almost all clinically available 1,352 bla genes with perfect specificity and sensitivity...
September 2016: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/27878209/preclinical-models-of-overwhelming-sepsis-implicate-the-neural-system-that-encodes-contextual-fear-memory
#7
Patricio T Huerta, Sergio Robbiati, Tomás Salvador Huerta, Anchal Sabharwal, Rose A Berlin, Maya Frankfurt, Bruce T Volpe
Long-term sepsis survivors sustain cryptic brain injury that leads to cognitive impairment, emotional imbalance, and increased disability burden. Suitable animal models of sepsis, such as cecal ligation and puncture (CLP), have permitted the analysis of abnormal brain circuits that underlie post-septic behavioral phenotypes. For instance, we have previously shown that CLP-exposed mice exhibit impaired spatial memory together with depleted dendritic arbors and decreased spines in the apical dendrites of pyramidal neurons in the CA1 region of the hippocampus...
November 17, 2016: Molecular Medicine
https://www.readbyqxmd.com/read/27873217/a-retrospective-evaluation-of-the-use-of-mass-spectrometry-in-fda-biologics-license-applications
#8
Sarah Rogstad, Anneliese Faustino, Ashley Ruth, David Keire, Michael Boyne, Jun Park
The characterization sections of biologics license applications (BLAs) approved by the United States Food and Drug Administration (FDA) between 2000 and 2015 were investigated to examine the extent of the use of mass spectrometry. Mass spectrometry was found to be integral to the characterization of these biotherapeutics. Of the 80 electronically submitted monoclonal antibody and protein biotherapeutic BLAs included in this study, 79 were found to use mass spectrometric workflows for protein or impurity characterization...
November 21, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27869181/functional-states-of-rat-cortical-circuits-during-the-unpredictable-availability-of-a-reward-related-cue
#9
Iván Fernández-Lamo, Raudel Sánchez-Campusano, Agnès Gruart, José M Delgado-García M
Proper performance of acquired abilities can be disturbed by the unexpected occurrence of external changes. Rats trained with an operant conditioning task (to press a lever in order to obtain a food pellet) using a fixed-ratio (1:1) schedule were subsequently placed in a Skinner box in which the lever could be removed randomly. Field postsynaptic potentials (fPSPs) were chronically evoked in perforant pathway-hippocampal CA1 (PP-CA1), CA1-subiculum (CA1-SUB), CA1-medial prefrontal cortex (CA1-mPFC), mPFC-nucleus accumbens (mPFC-NAc), and mPFC-basolateral amygdala (mPFC-BLA) synapses during lever IN and lever OUT situations...
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27865917/the-co-expression-of-glun2b-subunits-of-the-nmda-receptors-and-glucocorticoid-receptors-after-chronic-restraint-stress-in-low-and-high-anxiety-rats
#10
Małgorzata Lehner, Aleksandra Wisłowska-Stanek, Marek Gryz, Alicja Sobolewska, Danuta Turzyńska, Natalia Chmielewska, Paweł Krząścik, Anna Skórzewska, Adam Płaźnik
The aim of this study was to assess the mechanisms underlying behavioural differences between high- (HR) and low- (LR) anxiety rats, selected according to their behaviour in the contextual fear test (i.e., the duration of the freezing response was used as a discriminating variable), after a chronic restraint procedure (21days, 3h daily). We analysed the expression of the GluN2B subunits of the NMDA and glucocorticoid receptors (GRs) in selected brain structures (immunofluorescence). Following chronic restraint stress in the HR rats, we observed a decrease in the expression of the GRs and GluN2B subunits of the NMDA receptor in the prefrontal cortical areas and the hippocampus compared to the HR-control and the LR-restraint groups...
November 16, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/27862823/quinoidal-aromatic-transformation-in-%C3%AF-conjugated-oligomers-vibrational-raman-studies-on-the-limit-of-rupture-of-%C3%AF-bonds
#11
Juan Casado, Paula Mayorga Burrezo, José L Zafra, Juan T López Navarrete
The vibrational Raman spectra of several series of aromatic and quinoidal compounds have been analyzed considering the down-shifts and up-shifts of the frequencies of the relevant Raman bands as a function of the number of repeating units. Oligothiophenes, oligophenylene-vinylenes, oligoperylenes (oligophenyls) derivatives are studied in a common context. These shifts are taken as spectroscopic fingerprints of the changes in π-conjugation. For a given family, aromatic and quinoidal oligomers have been studied together and according with their Raman frequency shifts located in the two-well BLA-energy curve of their ground electronic state as a function of the bond-length-alternation pattern (BLA)...
November 11, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27862489/amygdala-mu-opioid-receptors-mediate-the-motivating-influence-of-cue-triggered-reward-expectations
#12
Nina T Lichtenberg, Kate M Wassum
Environmental reward-predictive stimuli can retrieve from memory a specific reward expectation that allows them to motivate action and guide choice. This process requires the basolateral amygdala (BLA), but little is known about the signaling systems necessary within this structure. Here we examined the role of the neuromodulatory opioid receptor system in the BLA in such cue-directed action using the outcome-specific Pavlovian-to-instrumental transfer (PIT) test in rats. Inactivation of BLA mu-, but not delta-opioid receptors was found to dose-dependently attenuate the ability of a reward-predictive cue to selectively invigorate the performance of actions directed at the same unique predicted reward (i...
November 12, 2016: European Journal of Neuroscience
https://www.readbyqxmd.com/read/27855079/chromosomal-amplification-of-the-blaoxa-58-carbapenemase-gene-in-a-proteus-mirabilis-clinical-isolate
#13
Delphine Girlich, Rémy A Bonnin, Pierre Bogaerts, Morgane De Laveleye, Daniel T Huang, Laurent Dortet, Philippe Glaser, Youri Glupczynski, Thierry Naas
Horizontal gene transfer may occur between distantly related bacteria thus leading to genetic plasticity and in some cases to acquisition of novel resistance traits. Proteus mirabilis is an Enterobacterial species responsible for human infections that may express various acquired ß-lactam resistance genes, including different classes of carbapenemase genes. Here we report a Proteus mirabilis clinical isolate (1091) displaying resistance to penicillin, including temocillin, together with reduced susceptibility to carbapenems and susceptibility to expanded-spectrum cephalosporins...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27845329/hyperconnectivity-of-prefrontal-cortex-to-amygdala-projections-in-a-mouse-model-of-macrocephaly-autism-syndrome
#14
Wen-Chin Huang, Youjun Chen, Damon T Page
Multiple autism risk genes converge on the regulation of mTOR signalling, which is a key effector of neuronal growth and connectivity. We show that mTOR signalling is dysregulated during early postnatal development in the cerebral cortex of germ-line heterozygous Pten mutant mice (Pten(+/-)), which model macrocephaly/autism syndrome. The basolateral amygdala (BLA) receives input from subcortical-projecting neurons in the medial prefrontal cortex (mPFC). Analysis of mPFC to BLA axonal projections reveals that Pten(+/-) mice exhibit increased axonal branching and connectivity, which is accompanied by increased activity in the BLA in response to social stimuli and social behavioural deficits...
November 15, 2016: Nature Communications
https://www.readbyqxmd.com/read/27845187/basolateral-amygdalar-d2-receptor-activation-is-required-for-the-companions-exerted-suppressive-effect-on-the-cocaine-conditioning
#15
Wen-Yu Tzeng, Chian-Fang G Cherng, Lung Yu, Ching-Yi Wang
The presence of companions renders decreases in cocaine-stimulated dopamine release in the nucleus accumbens and cocaine-induced conditioned place preference (CPP) magnitude. Limbic systems are widely believed to underlie the modulation of accumbal dopamine release and cocaine conditioning. Thus, this study aimed to assess whether intact basolateral nucleus of amygdala (BLA), dorsal hippocampus (DH), and dorsolateral striatum (DLS) is required for the companions-exerted suppressive effect on the cocaine-induced CPP...
November 11, 2016: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/27844053/involvement-of-crfr1-in-the-basolateral-amygdala-in-the-immediate-fear-extinction-deficit
#16
Fiona Hollis, Yannick Sevelinges, Jocelyn Grosse, Olivia Zanoletti, Carmen Sandi
Several animal and clinical studies have highlighted the ineffectiveness of fear extinction sessions delivered shortly after trauma exposure. This phenomenon, termed the immediate extinction deficit, refers to situations in which extinction programs applied shortly after fear conditioning may result in the reduction of fear behaviors (in rodents, frequently measured as freezing responses to the conditioned cue) during extinction training, but failure to consolidate this reduction in the long term. The molecular mechanisms driving this immediate extinction resistance remain unclear...
September 2016: ENeuro
https://www.readbyqxmd.com/read/27837411/first-study-on-characterization-of-virulence-and-antibiotic-resistance-genes-in-verotoxigenic-and-enterotoxigenic-e-coli-isolated-from-raw-milk-and-unpasteurized-traditional-cheeses-in-romania
#17
Alexandra Tabaran, Marian Mihaiu, Flaviu Tăbăran, Liora Colobatiu, Oana Reget, Mihai Marian Borzan, Sorin Daniel Dan
The study focused on the incidence of enterotoxigenic Escherichia coli (ETEC) and verotoxigenic E. coli (VTEC) in raw milk and traditional dairy cheeses marketed in Romania, characterizing the virulence and antibiotic resistance genes of these isolates. One hundred and twenty samples of raw milk and 80 samples of unpasteurized telemy cheese were collected and cultured according to the international standard protocol. All the characteristic E. coli cultures were analyzed for the presence of STa, STb, LT, stx1, and stx2 toxicity genes...
November 11, 2016: Folia Microbiologica
https://www.readbyqxmd.com/read/27829316/investigation-of-the-binding-interactions-of-bisdemethoxycurcumin-diacetylcurcumin-and-diacetylbisdemethoxycurcumin-with-bovine-%C3%AE-lactalbumin-by-experimental-and-theoretical-analysis
#18
Fakhrossadat Mohammadi, Marzieh Moeeni
It was reported that bovine α-lactalbumin (BLA) as an important whey protein can be utilized as valuable vehicle for metal ions. The goal of this study was to investigate the interaction of BLA with bisdemethoxycurcumin (BDMC), Diacetylcurcumin (DAC), and diacetylbisdemethoxycurcumin (DABC) as three bioactive compounds by fluorescence quenching measurements and docking studies. It was observed that these ligands come closer to tryptophan residues and quench their emission without any change in their micro region polarity...
December 1, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27825808/dissociation-in-the-effects-of-stress-and-d1-receptors-activation-on-basolateral-amygdalar-ltp-in-juvenile-and-adult-animals
#19
Rachel Schayek, Mouna Maroun
Post-weaning or juvenility is a critical developmental stage during which neural structures as the medial prefrontal cortex (mPFC) and amygdala and the neurotransmitter systems undergo major reorganization and thus are susceptible to the effects of environmental factors. Interactions between the mPFC and the amygdala are critical for fear regulation. Changes in plasticity in the projections from the mPFC to the basolateral amygdala (BLA) were previously shown following stress and fear. In the present study, we examined possible differences between adult and PW animals in the (1) effects of exposure to stress on BLA- LTP and (2) the possible dependency of mPFC-BLA LTP on N-methyl-d-aspartate (NMDA) and Dopamine (D) receptors activation...
November 4, 2016: Neuropharmacology
https://www.readbyqxmd.com/read/27822504/sleep-and-serotonin-modulate-paracapsular-nitric-oxide-synthase-expressing-neurons-of-the-amygdala
#20
Marco Bocchio, Simon P Fisher, Gunes Unal, Tommas J Ellender, Vladyslav V Vyazovskiy, Marco Capogna
Unraveling the roles of distinct neuron types is a fundamental challenge to understanding brain function in health and disease. In the amygdala, a brain structure regulating emotional behavior, the diversity of GABAergic neurons has been only partially explored. We report a novel population of GABAergic amygdala neurons expressing high levels of neuronal nitric oxide synthase (nNOS). These cells are predominantly localized along basolateral amygdala (BLA) boundaries. Performing ex vivo patch-clamp recordings from nNOS(+) neurons in Nos1-Cre(ER);Ai9 mice, we observed that nNOS(+) neurons located along the external capsule display distinctive electrophysiological properties, axonal and dendritic arborization, and connectivity...
September 2016: ENeuro
keyword
keyword
108376
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"