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https://www.readbyqxmd.com/read/28645211/akr1c3-mediated-adipose-androgen-generation-drives-lipotoxicity-in-women-with-polycystic-ovary-syndrome
#1
Michael W O'Reilly, Punith Kempegowda, Mark Walsh, Angela E Taylor, Konstantinos N Manolopoulos, J William Allwood, Robert K Semple, Daniel Hebenstreit, Warwick B Dunn, Jeremy W Tomlinson, Wiebke Arlt
Context: Polycystic ovary syndrome (PCOS) is a prevalent metabolic disorder, occurring in up to 10% of women of reproductive age. PCOS is associated with insulin resistance and cardiovascular risk. Androgen excess is a defining feature of PCOS and has been suggested as causally associated with insulin resistance; however, mechanistic evidence linking both is lacking. We hypothesized that adipose tissue is an important site linking androgen activation and metabolic dysfunction in PCOS...
June 22, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28645179/system-wide-analysis-of-short-term-response-to-high-temperature-in-pinus-radiata
#2
Mónica Escandón, Luis Valledor, Jesús Pascual, Gloria Pinto, María Jesús Cañal, Mónica Meijón
Pinus radiata seedlings, the most widely planted pine species in the world, were exposed to temperatures within a range mimicking future scenarios based on current models of heat increase. The short-term heat response in P. radiata was studied in detail by exploring the metabolome, proteome and targeted transcriptome. The use of complementary mass spectrometry techniques, GC-MS and LC-Orbitrap-MS, together with novel bioinformatics tools allowed the reliable quantification of 2,075 metabolites and 901 protein groups...
June 22, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28644610/mzmine-2-data-preprocessing-to-enhance-molecular-networking-reliability
#3
Florent Olivon, Gwendal Grelier, Fanny Roussi, Marc Litaudon, David Touboul
Molecular networking is becoming more and more popular into the metabolomic community to organize tandem mass spectrometry (MS2) data. Even though this approach allows the treatment and comparison of large data sets, several drawbacks related to the MS-Cluster tool routinely used on the Global Natural Product Social Molecular Networking platform (GNPS) limit its potential. MS-Cluster cannot distinguish between chromatography well-resolved isomers as retention times are not taken into account. Annotation with predicted chemical formulas is also not implemented and semi-quantification is only based on the number of MS2 scans...
June 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28644019/metabolomics-analysis-to-evaluate-the-anti-inflammatory-effects-of-polyphenols-glabridin-reversed-metabolism-change-caused-by-lps-in-raw-264-7-cells
#4
Kaiqin Liu, FuWei Pi, Hongxia Zhang, Jian Ji, Shuang Xia, Fangchao Cui, Jiadi Sun, Xiulan Sun
Inflammation has been shown to play a critical role in the development of many diseases. In this study, we used metabolomics to evaluate the inflammatory effect of lipopolysaccharide (LPS) and anti-inflammatory effect of glabridin (GB, a polyphenol from Glycurrhiza glabra L. roots) in RAW 264.7 cells. Multivariate statistical analysis showed that compared with the LPS group, the metabolic profile of the GB group was more similar to that of the control group. LPS impacted the amino acid, energy and lipid metabolisms in RAW 264...
June 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28643618/heat-stabilised-rice-bran-consumption-by-colorectal-cancer-survivors-modulates-stool-metabolite-profiles-and-metabolic-networks-a-randomised-controlled-trial
#5
Dustin G Brown, Erica C Borresen, Regina J Brown, Elizabeth P Ryan
Rice bran (RB) consumption has been shown to reduce colorectal cancer (CRC) growth in mice and modify the human stool microbiome. Changes in host and microbial metabolism induced by RB consumption was hypothesised to modulate the stool metabolite profile in favour of promoting gut health and inhibiting CRC growth. The objective was to integrate gut microbial metabolite profiles and identify metabolic pathway networks for CRC chemoprevention using non-targeted metabolomics. In all, nineteen CRC survivors participated in a parallel randomised controlled dietary intervention trial that included daily consumption of study-provided foods with heat-stabilised RB (30 g/d) or no additional ingredient (control)...
May 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28643516/untargeted-comprehensive-two-dimensional-liquid-chromatography-coupled-with-high-resolution-mass-spectrometry-analysis-of-rice-metabolome-using-multivariate-curve-resolution
#6
Meritxell Navarro-Reig, Joaquim Jaumot, Anna Baglai, Gabriel Vivó-Truyols, Peter J Schoenmakers, Romà Tauler
In this work, a new strategy for the chemometric analysis of LC×LC-HRMS data is proposed. This approach consists of a preliminary compression step along the MS spectral dimension based on the selection of the Regions of Interest (ROI), followed by a further data compression along the chromatographic dimension by wavelet transforms. In a secondary step, the Multivariate Curve Resolution Alternating Least Squares (MCR-ALS) method is applied to previously compressed data sets obtained in the simultaneous analysis of multiple LC×LC-HRMS chromatographic runs from multiple samples...
June 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28643099/-beyond-antibiotic-therapy-future-antiinfective-strategies-update-2017
#7
REVIEW
D Vogt, S Sperling, T Tkhilaishvili, A Trampuz, J-P Pirnay, C Willy
BACKGROUND: The key elements in the therapy of surgical site infections (SSI) are surgical debridement and local and systemic antibiotic therapy; however, due to increasing antibiotic resistance, the development of additional therapeutic measures is of great interest for future trauma and orthopedic surgery. METHOD: Against the background of our own experimental and clinical experiences and on the basis of the current literature, possible future anti-infective strategies were elaborated...
June 22, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28642837/environmental-influences-in-the-etiology-of-colorectal-cancer-the-premise-of-metabolomics
#8
Nicholas J W Rattray, Georgia Charkoftaki, Zahra Rattray, James E Hansen, Vasilis Vasiliou, Caroline H Johnson
PURPOSE OF REVIEW: In this review we discuss how environmental exposures predominate the etiology of colorectal cancer (CRC). With CRC being a personalized disease influenced by genes and environment, our goal was to explore the role metabolomics can play in identifying exposures, assessing the interplay between co-exposures, and the development of personalized therapeutic interventions. RECENT FINDINGS: Approximately 10 % of CRC cases can be explained by germ-line mutations, whereas the prevailing majority are caused by an initiating exposure event occurring decades prior to diagnosis...
June 2017: Current Pharmacology Reports
https://www.readbyqxmd.com/read/28642462/metabolic-changes-in-medaka-fish-induced-by-cyanobacterial-exposures-in-mesocosms-an-integrative-approach-combining-proteomic-and-metabolomic-analyses
#9
Benoît Sotton, Alain Paris, Séverine Le Manach, Alain Blond, Gérard Lacroix, Alexis Millot, Charlotte Duval, Hélène Huet, Qin Qiao, Sophie Labrut, Giovanni Chiapetta, Joelle Vinh, Arnaud Catherine, Benjamin Marie
Cyanobacterial blooms pose serious threats to aquatic organisms and strongly impact the functioning of aquatic ecosystems. Due to their ability to produce a wide range of potentially bioactive secondary metabolites, so called cyanotoxins, cyanobacteria have been extensively studied in the past decades. Proteomic and metabolomic analyses provide a unique opportunity to evaluate the global response of hundreds of proteins and metabolites at a glance. In this study, we provide the first combined utilization of these methods targeted to identify the response of fish to bloom-forming cyanobacteria...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28642310/metabolomics-analysis-identifies-sex-associated-metabotypes-of-oxidative-stress-and-the-autotaxin-lysopa-axis-in%C3%A2-copd
#10
Shama Naz, Johan Kolmert, Mingxing Yang, Stacey N Reinke, Muhammad Anas Kamleh, Stuart Snowden, Tina Heyder, Bettina Levänen, David J Erle, C Magnus Sköld, Åsa M Wheelock, Craig E Wheelock
Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease and a leading cause of mortality and morbidity worldwide. The aim of this study was to investigate the sex dependency of circulating metabolic profiles in COPD.Serum from healthy never-smokers (healthy), smokers with normal lung function (smokers), and smokers with COPD (COPD; Global Initiative for Chronic Obstructive Lung Disease stages I-II/A-B) from the Karolinska COSMIC cohort (n=116) was analysed using our nontargeted liquid chromatography-high resolution mass spectrometry metabolomics platform...
June 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28642031/clustering-of-ms-2-spectra-using-unsupervised-methods-to-aid-the-identification-of-secondary-metabolites-from-pseudomonas-aeruginosa
#11
Tobias Depke, Raimo Franke, Mark Brönstrup
Pseudomonas aeruginosa is an important opportunistic pathogen that produces a large arsenal of small molecule virulence factors and quorum sensing signal molecules. The annotation of these secondary metabolites in untargeted, mass spectrometry-based metabolomics is difficult, as many of them cannot be found in common metabolite databases, and as manual annotation is tedious. We therefore developed an algorithm named CluMSID that uses cosine similarities of product ion spectra and neutral loss patterns in combination with unsupervised clustering methods such as multidimensional scaling, density based clustering and hierarchical clustering to group structurally similar compounds and hence facilitate their annotation...
June 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28641781/pregnancy-to-postpartum-transition-of-serum-metabolites-in-women-with-gestational-diabetes
#12
Elin Chorell, Ulrika Andersson Hall, Carolina Gustavsson, Kerstin Berntorp, Jatta Puhkala, Riitta Luoto, Tommy Olsson, Agneta Holmäng
CONTEXT: Gestational diabetes is commonly linked to development of type 2 diabetes mellitus (T2DM). There is a need to characterize metabolic changes associated with gestational diabetes in order to find novel biomarkers for T2DM. OBJECTIVE: To find potential pathophysiological mechanisms and markers for progression from gestational diabetes mellitus to T2DM by studying the metabolic transition from pregnancy to postpartum. DESIGN: The metabolic transition profile from pregnancy to postpartum was characterized in 56 women by mass spectrometry-based metabolomics; 11 women had gestational diabetes mellitus, 24 had normal glucose tolerance, and 21 were normoglycaemic but at increased risk for gestational diabetes mellitus...
July 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28641411/development-of-an-lc-hrms-metabolomics-method-with-high-specificity-for-metabolite-identification-using-all-ion-fragmentation-aif-acquisition
#13
Shama Naz, Hector Gallart-Ayala, Stacey Reinke, Caroline Mathon, Richard Blankley, Romanas Chaleckis, Craig Edward Wheelock
High-resolution mass spectrometry (HRMS)-based metabolomics approaches have made significant advances. However, metabolite identification is still a major challenge and significant bottleneck in translating metabolomics data into biological context. In the current study, a liquid chromatography (LC)-HRMS metabolomics method was developed using an all ion fragmentation (AIF) acquisition approach. In order to increase the specificity in metabolite annotation, four criteria were considered: i) accurate mass (AM), ii) retention time (RT), iii) MS/MS spectrum, and iv) product/precursor ion intensity ratios...
June 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28640973/production-of-red-flowered-oilseed-rape-via-the-ectopic-expression-of-orychophragmus-violaceus-ovpap2
#14
Wenqin Fu, Daozong Chen, Qi Pan, Fengfeng Li, Zhigang Zhao, Xianhong Ge, Zaiyun Li
Oilseed rape (Brassica napus L.), which has yellow flowers, is both an important oil crop and a traditional tourism resource in China, whereas the Orychophragmus violaceus, which has purple flowers, likely possesses a candidate gene or genes to alter the flower color of oilseed rape. A previously established B. napus line has a particular pair of O. violaceus chromosomes (M4) and exhibits slightly red petals. In this study, the transcriptomic analysis of M4, B. napus (H3), and O. violaceus with purple petals (OvP) and with white petals (OvW) revealed that most anthocyanin biosynthesis genes were up-regulated in both M4 and OvP...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28640868/functional-metabolomics-as-a-tool-to-analyze-mediator-function-and-structure-in-plants
#15
Celine Davoine, Ilka N Abreu, Khalil Khajeh, Jeanette Blomberg, Brendan N Kidd, Kemal Kazan, Peer M Schenk, Lorenz Gerber, Ove Nilsson, Thomas Moritz, Stefan Björklund
Mediator is a multiprotein transcriptional co-regulator complex composed of four modules; Head, Middle, Tail, and Kinase. It conveys signals from promoter-bound transcriptional regulators to RNA polymerase II and thus plays an essential role in eukaryotic gene regulation. We describe subunit localization and activities of Mediator in Arabidopsis through metabolome and transcriptome analyses from a set of Mediator mutants. Functional metabolomic analysis based on the metabolite profiles of Mediator mutants using multivariate statistical analysis and heat-map visualization shows that different subunit mutants display distinct metabolite profiles, which cluster according to the reported localization of the corresponding subunits in yeast...
2017: PloS One
https://www.readbyqxmd.com/read/28640802/peroxisomal-biogenesis-is-genetically-and-biochemically-linked-to-carbohydrate-metabolism-in-drosophila-and-mouse
#16
Michael F Wangler, Yu-Hsin Chao, Vafa Bayat, Nikolaos Giagtzoglou, Abhijit Babaji Shinde, Nagireddy Putluri, Cristian Coarfa, Taraka Donti, Brett H Graham, Joseph E Faust, James A McNew, Ann Moser, Marco Sardiello, Myriam Baes, Hugo J Bellen
Peroxisome biogenesis disorders (PBD) are a group of multi-system human diseases due to mutations in the PEX genes that are responsible for peroxisome assembly and function. These disorders lead to global defects in peroxisomal function and result in severe brain, liver, bone and kidney disease. In order to study their pathogenesis we undertook a systematic genetic and biochemical study of Drosophila pex16 and pex2 mutants. These mutants are short-lived with defects in locomotion and activity. Moreover these mutants exhibit severe morphologic and functional peroxisomal defects...
June 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28640714/an-unexplored-brain-gut-microbiota-axis-in-stroke
#17
Shu Wen Wen, Connie H Y Wong
Microbiota research, in particular that of the gut, has recently gained much attention in medical research owing to technological advances in metagenomics and metabolomics. Despite this, much of the research direction has focused on long-term or chronic effects of microbiota manipulation on health and disease. In this addendum, we reflect on our recent publication that reported findings addressing a rather unconventional hypothesis. Bacterial pneumonia is highly prevalent and is one of the leading contributors to stroke morbidity and mortality worldwide...
June 22, 2017: Gut Microbes
https://www.readbyqxmd.com/read/28640361/global-and-targeted-serum-metabolic-profiling-of-colorectal-cancer-progression
#18
Yin Long, Beatriz Sanchez-Espiridion, Moubin Lin, Lindsey White, Lopa Mishra, Gottumakkala S Raju, Scott Kopetz, Cathy Eng, Michelle A T Hildebrandt, David W Chang, Yuanqing Ye, Dong Liang, Xifeng Wu
BACKGROUND: Patients with colorectal adenoma polyps (PLPs) are at higher risk for developing colorectal cancer (CRC). However, the development of improved and robust biomarkers to enable the screening, surveillance, and early detection of PLPs and CRC continues to be a challenge. The aim of this study was to identify biomarkers of progression to CRC through metabolomic profiling of human serum samples with a multistage approach. METHODS: Metabolomic profiling was conducted with the Metabolon platform for 30 CRC patients, 30 PLP patients, and 30 control subjects, and this was followed by the targeted validation of the top metabolites in an additional set of 50 CRC patients, 50 PLP patients, and 50 controls with liquid chromatography-tandem mass spectrometry...
June 22, 2017: Cancer
https://www.readbyqxmd.com/read/28640228/development-and-validation-of-a-high-throughput-mass-spectrometry-based-urine-metabolomic-test-for-the-detection-of-colonic-adenomatous-polyps
#19
Lu Deng, David Chang, Rae R Foshaug, Roman Eisner, Victor K Tso, David S Wishart, Richard N Fedorak
Background: Colorectal cancer is one of the leading causes of cancer deaths worldwide. The detection and removal of the precursors to colorectal cancer, adenomatous polyps, is the key for screening. The aim of this study was to develop a clinically scalable (high throughput, low cost, and high sensitivity) mass spectrometry (MS)-based urine metabolomic test for the detection of adenomatous polyps. Methods: Prospective urine and stool samples were collected from 685 participants enrolled in a colorectal cancer screening program to undergo colonoscopy examination...
June 22, 2017: Metabolites
https://www.readbyqxmd.com/read/28640223/non-targeted-metabolomics-analysis-of-the-effects-of-tyrosine-kinase-inhibitors-sunitinib-and-erlotinib-on-heart-muscle-liver-and-serum-metabolism-in-vivo
#20
Brian C Jensen, Traci L Parry, Wei Huang, Amro Ilaiwy, James R Bain, Michael J Muehlbauer, Sara K O'Neal, Cam Patterson, Gary L Johnson, Monte S Willis
Background: More than 90 tyrosine kinases have been implicated in the pathogenesis of malignant transformation and tumor angiogenesis. Tyrosine kinase inhibitors (TKIs) have emerged as effective therapies in treating cancer by exploiting this kinase dependency. The TKI erlotinib targets the epidermal growth factor receptor (EGFR), whereas sunitinib targets primarily vascular endothelial growth factor receptor (VEGFR) and platelet-derived growth factor receptor (PDGFR).TKIs that impact the function of non-malignant cells and have on- and off-target toxicities, including cardiotoxicities...
June 22, 2017: Metabolites
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