Sarthak Gupta, Eiko Yamada, Hiroyuki Nakamura, Paola Perez, Thomas Jf Pranzatelli, Kalie Dominick, Shyh-Ing Jang, Mehdi Abed, Daniel Martin, Peter Burbelo, ChangYu Zheng, Ben French, Ilias Alevizos, Zohreh Khavandgar, Margaret Beach, Eileen Pelayo, Brian Walitt, Sarfaraz Hasni, Mariana J Kaplan, Mayank Tandon, Maria Teresa Magone, David E Kleiner, John A Chiorini, Alan Baer, Blake M Warner
OBJECTIVES: Inflammatory cytokines that signal through the Janus kinases-signal transducer and activator of transcription (JAK-STAT) pathway, especially interferons (IFNs), are implicated in Sjögren's disease (SjD). Although inhibition of JAKs is effective in other autoimmune diseases, a systematic investigation of IFN-JAK-STAT signalling and the effect of JAK inhibitor (JAKi) therapy in SjD-affected human tissues has not been fully investigated. METHODS: Human minor salivary glands (MSGs) and peripheral blood mononuclear cells (PBMCs) were investigated using bulk or single-cell (sc) RNA sequencing (RNAseq), immunofluorescence (IF) microscopy and flow cytometry...
March 25, 2024: Annals of the Rheumatic Diseases