Xueli Ren, Hironori Tsuji, Takahiko Uchino, Izumi Kono, Takashi Isoshima, Akimitsu Okamoto, Noriyuki Nagaoka, Toshifumi Ozaki, Akihiro Matsukawa, Hideyuki Miyatake, Yoshihiro Ito
Inorganic biomaterials are used in various orthopedic and dental implants. Nevertheless, they cause clinical issues such as loosening of implants and patient morbidity. Therefore, inspired by mussel adhesive proteins, we aimed to design an adhesive and dimer-forming highly active bone morphogenetic protein-2 (BMP-2) using bioorthogonal chemistry, in which recombinant DNA technology was combined with enzymatic modifications, to achieve long-term osseointegration with titanium. The prepared BMP-2 exhibited substantially higher binding activity than wild-type BMP-2, while the adhered BMP-2 was more active than soluble BMP-2...
March 7, 2024: Journal of Materials Chemistry. B, Materials for Biology and Medicine