keyword
https://read.qxmd.com/read/38533617/cooperative-dynamics-of-dna-grafted-magnetic-nanoparticles-optimize-magnetic-biosensing-and-coupling-to-dna-origami
#1
JOURNAL ARTICLE
Aidin Lak, Yihao Wang, Pauline J Kolbeck, Christoph Pauer, Mohammad Suman Chowdhury, Marco Cassani, Frank Ludwig, Thilo Viereck, Florian Selbach, Philip Tinnefeld, Meinhard Schilling, Tim Liedl, Joe Tavacoli, Jan Lipfert
Magnetic nanoparticles (MNPs) provide new opportunities for enzyme-free biosensing of nucleic acid biomarkers and magnetic actuation by patterning on DNA origami, yet how the DNA grafting density affects their dynamics and accessibility remains poorly understood. Here, we performed surface functionalization of MNPs with single-stranded DNA (ssDNA) via click chemistry with a tunable grafting density, which enables the encapsulation of single MNPs inside a functional polymeric layer. We used several complementary methods to show that particle translational and rotational dynamics exhibit a sigmoidal dependence on the ssDNA grafting density...
March 27, 2024: Nanoscale
https://read.qxmd.com/read/38529703/concept-and-development-of-algebraic-topological-framework-nucleic-acids
#2
JOURNAL ARTICLE
Lu Song, Xiaolei Zuo, Min Li
Nucleic acids are considered as promising materials for developing exquisite nanostructures from one to three dimensions. The advances of DNA nanotechnology facilitate ingenious design of DNA nanostructures with diverse shapes and sizes. Especially, the algebraic topological framework nucleic acids (ATFNAs) are functional DNA nanostructures that engineer guest molecules (e.g., nucleic acids, proteins, small molecules, and nanoparticles) stoichiometrically and spatially. The intrinsic precise properties and tailorable functionalities of ATFNAs hold great promise for biological applications, such as cell recognition and immunotherapy...
March 26, 2024: ChemPlusChem
https://read.qxmd.com/read/38522375/effect-of-the-combined-binding-of-topotecan-and-catechin-protocatechuic-acid-to-a-ph-sensitive-dna-tetrahedron-on-release-and-cytotoxicity-spectroscopic-and-calorimetric-studies
#3
JOURNAL ARTICLE
Lu Wang, Jie Liu, Xiangtai Wang, Xinyu Li, Xinpeng Zhang, Lixia Yuan, Yushu Wu, Min Liu
The therapeutic efficacy of chemotherapy drugs can be effectively improved through the dual effects of their combination with natural polyphenols and the delivery of targeted DNA nanostructures. In this work, the interactions of topotecan (TPT), (+)-catechin (CAT), or protocatechuic acid (PCA) with a pH-sensitive DNA tetrahedron (MUC1-TD) in the binary and ternary systems at pHs 5.0 and 7.4 were investigated by fluorescence spectroscopy and calorimetry. The intercalative binding mode of TPT/CAT/PC to MUC1-TD was confirmed, and their affinity was ranked in the order of PCA > CAT > TPT...
March 20, 2024: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://read.qxmd.com/read/38521021/-two-in-one-a-novel-dna-cascade-amplification-strategy-for-trace-detection-of-dual-targets
#4
JOURNAL ARTICLE
Qianqian Cai, Yuehui Wang, Yuanzhen Ning, Guifen Jie
According to the characteristics of DNA programming, the cascaded nucleic acid amplification technology with larger output can overcome the problem of insufficient sensitivity of single nucleic acid amplification technology, and it combines the advantages of two or even multiple nucleic acid amplification technologies at the same time. In this work, a novel cascade signal amplification strategy with strand displacement amplification (SDA) and cascade hybridization chain reaction (HCR) was proposed for trace detection of hAAG and VEGF165...
March 21, 2024: Talanta
https://read.qxmd.com/read/38517264/modular-weaving-dnazyme-in-skeleton-of-dna-nanocages-for-photoactivatable-catalytic-activity-regulation
#5
JOURNAL ARTICLE
Hengzhi Zhao, Deyu Yi, Lele Li, Yuliang Zhao, Mengyuan Li
DNAzymes exhibit tremendous application potentials in the field of biosensing and gene regulation due to its unique catalytic function. However, spatiotemporally controlled regulation of DNAzyme activity remains a daunting challenge, which may cause nonspecific signal leakage or gene silencing of the catalytic systems. Here, we report a photochemical approach via modular weaving active DNAzyme into the skeleton of tetrahedral DNA nanocages (TDN) for light-triggered on-demand liberation of DNAzyme and thus conditional control of gene regulation activity...
March 22, 2024: Angewandte Chemie
https://read.qxmd.com/read/38512069/rapid-silicification-of-a-dna-origami-with-shape-fidelity
#6
JOURNAL ARTICLE
Haozhi Wang, Ziyu Li, Xiaoguo Liu, Sisi Jia, Yanjing Gao, Mingqiang Li
High-fidelity patterning of DNA origami nanostructures on various interfaces holds great potential for nanoelectronics and nanophotonics. However, distortion of a DNA origami often occurs due to the strong interface interactions, e.g., on two-dimensional (2D) materials. In this study, we discovered that the adsorption of silica precursors in rapid silicification can prevent the distortion caused by graphene and generates a high shape-fidelity DNA origami-silica composite on a graphene interface. We found that an incubation time of 1 min and silicification time of 16 h resulted in the formation of DNA origami-silica composites with the highest shape fidelity of 99%...
March 21, 2024: ACS Applied Bio Materials
https://read.qxmd.com/read/38505984/selectivity-in-the-chiral-self-assembly-of-nucleobase-arylazopyrazole-photoswitches-along-dna-templates
#7
JOURNAL ARTICLE
Noemí Nogal, Santiago Guisán, David Dellemme, Mathieu Surin, Andrés de la Escosura
The control of supramolecular DNA assembly through external stimuli such as light represents a promising approach to control bioreactions, and modulate hybridization or delivery processes. Here, we report on the design of nucleobase-containing arylazopyrazole photoswitches that undergo chiral organization upon self-assembly along short DNA templates. Chiroptical spectroscopy shows that the specific nucleobases allow selectivity in the resulting supramolecular DNA complexes, and UV light irradiation triggers partial desorption of the arylazopyrazole photoswitches...
March 20, 2024: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://read.qxmd.com/read/38502035/trehalose-polyamine-dna-nanocomplexes-impact-of-vector-architecture-on-cell-and-organ-transfection-selectivity
#8
JOURNAL ARTICLE
Fernando Ortega-Caballero, María L Santana-Armas, Conchita Tros de Ilarduya, Christophe Di Giorgio, Raphäel Tripier, Nathalie Le Bris, Cedric Ollier, Carmen Ortiz Mellet, José M García Fernández, José L Jiménez Blanco, Alejandro Méndez-Ardoy
A novel family of precision-engineered gene vectors with well-defined structures built on trehalose and trehalose-based macrocycles (cyclotrehalans) comprising linear or cyclic polyamine heads have been synthesized through procedures that exploit click chemistry reactions. The strategy was conceived to enable systematic structural variations and, at the same time, ensuring that enantiomerically pure vectors are obtained. Notably, changes in the molecular architecture translated into topological differences at the nanoscale upon co-assembly with plasmid DNA, especially regarding the presence of regions with short- or long-range internal order as observed by TEM...
March 19, 2024: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://read.qxmd.com/read/38497300/cell-surface-binding-of-dna-nanostructures-for-enhanced-intracellular-and-intranuclear-delivery
#9
JOURNAL ARTICLE
Weitao Wang, Bhavya Chopra, Vismaya Walawalkar, Zijuan Liang, Rebekah Adams, Markus Deserno, Xi Ren, Rebecca E Taylor
DNA nanostructures (DNs) have found increasing use in biosensing, drug delivery, and therapeutics because of their customizable assembly, size and shape control, and facile functionalization. However, their limited cellular uptake and nuclear delivery have hindered their effectiveness in these applications. Here, we demonstrate the potential of applying cell-surface binding as a general strategy to enable rapid enhancement of intracellular and intranuclear delivery of DNs. By targeting the plasma membrane via cholesterol anchors or the cell-surface glycocalyx using click chemistry, we observe a significant 2 to 8-fold increase in the cellular uptake of three distinct types of DNs that include nanospheres, nanorods, and nanotiles, within a short time frame of half an hour...
March 18, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38492362/label-free-plasmonic-spectral-profiling-of-serum-dna
#10
JOURNAL ARTICLE
Peng Zheng, Piyush Raj, Le Liang, Lintong Wu, Santosh Kumar Paidi, Jeong Hee Kim, Ishan Barman
Genetic and epigenetic modifications are linked to the activation of oncogenes and inactivation of tumor suppressor genes. Likewise, the associated molecular alternations can best inform precision medicine for personalized tumor treatment. Therefore, performing characterization of genetic and epigenetic alternations at the molecular level represents a crucial step in early diagnosis and/or therapeutics of cancer. However, the prevailing methods for DNA analysis involve a series of tedious and complicated steps, in which important genetic and epigenetic information could be lost or altered...
March 13, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38490809/on-demand-crack-formation-on-dna-film-via-organic-solvent-induced-dehydration
#11
JOURNAL ARTICLE
Soeun Lee, Hyeonbin Moon, Juri Kim, Seunghwa Ryu, Soon Mo Park, Dong Ki Yoon
Crack is found on the soil when severe drought comes, which inspired the idea to rationalize patterning applications using dried deoxyribonucleic acid (DNA) film. DNA is one of the massively produced biomaterials in nature, showing the lyotropic liquid crystal (LC) phase in highly concentrated conditions. DNA nanostructures in the hydrated condition can be orientation controlled, which can be extended to make drying-induced cracks. We explore the controlled crack generation in oriented DNA films by inducing mechanical fracture through organic solvent-induced dehydration (OSID) using tetrahydrofuran (THF)...
March 15, 2024: Advanced Materials
https://read.qxmd.com/read/38490020/in-vitro-detection-of-circulating-tumor-cells-using-the-nicking-endonuclease-assisted-lanthanide-metal-luminescence-amplification-strategy
#12
JOURNAL ARTICLE
Xuekang Wang, Yating Zeng, Nanhang Zhu, Yue Yu, Qiangying Yi, Yao Wu
The in vitro detection of circulating tumor cells (CTCs) has been proven as a vital method for early diagnosis and evaluation of cancer metastasis, since the existence and number fluctuation of CTCs have shown close correlation with clinical outcomes. However, it remains difficult and technically challenging to realize accurate CTCs detection, due to the rarity of CTCs in the blood samples with complex components. Herein, we reported a CTCs in vitro detection strategy, utilizing a loop amplification strategy based on DNA tetrahedron and nicking endonuclease reaction, as well as the anti-background interference based on lanthanide metal luminescence strategy...
March 14, 2024: Talanta
https://read.qxmd.com/read/38488889/tobramycin-mediated-self-assembly-of-dna-nanostructures-for-targeted-treatment-of-pseudomonas-aeruginosa-infected-lung-inflammation
#13
JOURNAL ARTICLE
Yuhang Xu, Qian Liu, Bin Wang, Quan Li, Yue Chen, Yao Yang, Zhihao Zhu, Daohui Gong, Chuan Zhang, Guansong Wang, Hang Qian
Pseudomonas aeruginosa (PA) is one of the most common multidrug-resistant pathogens found in clinics, often manifesting as biofilms. However, due to the emergence of superbugs in hospitals and the overuse of antibiotics, the prevention and treatment of PA infections have become increasingly challenging. Utilizing DNA nanostructures for packaging and delivering antibiotics presents an intervention strategy with significant potential. Nevertheless, construction of functional DNA nanostructures with multiple functionalities and enhanced stability in physiological settings remains challenging...
March 15, 2024: Biomaterials Science
https://read.qxmd.com/read/38482742/programming-of-supercrystals-using-replicable-dna-functionalized-colloids
#14
JOURNAL ARTICLE
Xiaoyun Sun, Wenqiang Hua, Xiaoyu Liu, Jing Jin, Jianing Zhang, Jie Tian, Bin Zheng, Wei Jiang, Dongbao Yao, Haojun Liang
The development of self-replicating systems is of great importance in research on the origin of life. As the most iconic molecules, nucleic acids have provided prominent examples of the fabrication of self-replicating artificial nanostructures. However, it is still challenging to construct sophisticated synthetic systems that can create large-scale or three-dimensionally ordered nanomaterials using self-replicating nanostructures. By integrating a template system containing DNA-functionalized colloidal seeds with a simplified DNA strand-displacement circuit programmed subsystem to produce DNA-functionalized colloidal copies, we developed a facile enthalpy-mediated strategy to control the replication and catalytic assembly of DNA-functionalized colloids in a time-dependent manner...
March 14, 2024: Angewandte Chemie
https://read.qxmd.com/read/38481552/curcumin-in-treatment-of-hematological-cancers-promises-and-challenges
#15
REVIEW
Maliheh Entezari, Armita Tayari, Mahshid Deldar Abad Paskeh, Simin Khorsand Kheirabad, Sahar Naeemi, Afshin Taheriazam, Hossein Dehghani, Shokooh Salimimoghadam, Mehrdad Hashemi, Sepideh Mirzaei, Saeed Samarghandian
Hematological cancers include leukemia, myeloma and lymphoma and up to 178.000 new cases are diagnosed with these tumors each year. Different kinds of treatment including radiotherapy, chemotherapy, immunotherapy and stem cell transplantation have been employed in the therapy of hematological cancers. However, they are still causing death among patients. On the other hand, curcumin as an anti-cancer agent for the suppression of human cancers has been introduced. The treatment of hematological cancers using curcumin has been followed...
March 2024: Journal of Traditional and Complementary Medicine
https://read.qxmd.com/read/38477549/functional-2d-nanoplatforms-alleviate-eosinophilic-chronic-rhinosinusitis-by-modulating-eosinophil-extracellular-trap-formation
#16
JOURNAL ARTICLE
Zhaoxu Tu, Ming Liu, Changyi Xu, Yi Wei, Tong Lu, Yongqiang Xiao, Hongxia Li, Shuaiyin Zhang, Xinran Xie, Jian Li, Weiping Wen
The therapeutic outcomes of patients with eosinophilic chronic rhinosinusitis (ECRS) remain unsatisfactory, largely because the underlying mechanisms of eosinophilic inflammation are uncertain. Here, it is shown that the nasal secretions of ECRS patients have high eosinophil extracellular trap (EET) and cell-free DNA (cfDNA) levels. Moreover, the cfDNA induced EET formation by activating toll-like receptor 9 (TLR9) signaling. After demonstrating that DNase I reduced eosinophilic inflammation by modulating EET formation, linear polyglycerol-amine (LPGA )-coated TiS2 nanosheets (TLPGA ) as functional 2D nanoplatforms with low cytotoxicity, mild protein adsorption, and increased degradation rate is developed...
March 13, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/38470720/a-curcumin-decorated-nanozyme-with-ros-scavenging-and-anti-inflammatory-properties-for-neuroprotection
#17
JOURNAL ARTICLE
Feng Gao, Wenyu Liang, Qixin Chen, Bairu Chen, Yuchen Liu, Zhibo Liu, Xu Xu, Rongrong Zhu, Liming Cheng
Disordered reactive oxygen/nitrogen species are a common occurrence in various diseases, which usually cause cellular oxidative damage and inflammation. Despite the wide range of applications for biomimetic nanoparticles with antioxidant or anti-inflammatory properties, designs that seamlessly integrate these two abilities with a synergistic effect in a simple manner are seldom reported. In this study, we developed a novel PEI-Mn composite nanoparticle (PM NP) using a chelation method, and the curcumin was loaded onto PM NPs via metal-phenol coordination to form PEI-Mn@curcumin nanoparticles (PMC NPs)...
February 20, 2024: Nanomaterials
https://read.qxmd.com/read/38464301/mimicking-an-in-cellulo-environment-for-enzyme-free-paper-based-nucleic-acid-tests-at-the-point-of-care
#18
Jeffrey W Beard, Samuel L Hunt, Alexander Evans, Coleman Goenner, Benjamin L Miller
Point of care (PoC) nucleic acid amplification tests (NAATs) are a cornerstone of public health, providing the earliest and most accurate diagnostic method for many communicable diseases, such as HIV, in the same location the patient receives treatment. Communicable diseases disproportionately impact low-resource communities where NAATs are often unobtainable due to the resource intensive enzymes that drive the tests. Enzyme-free nucleic acid detection methods, such as hybridization chain reaction (HCR), use DNA secondary structures for self-driven amplification schemes producing large DNA nanostructures and capable of single molecule detection in cellulo ...
February 29, 2024: bioRxiv
https://read.qxmd.com/read/38457775/plasmonic-gold-nanostar-based-probes-with-distance-dependent-plasmon-enhanced-fluorescence-for-ultrasensitive-dna-methyltransferase-assay
#19
JOURNAL ARTICLE
Tingting Zhao, Xiaozhe Pang, Congkai Wang, Lei Wang, Yifan Yang, Junqi Wang, Jiangfei Jia, Xinxue Liu, Shenghao Xu, Xiliang Luo
The ultrasensitive DNA methyltransferase (Dam MTase) assay is of high significance for biomedical research and clinical diagnosis because of its profound effect on gene regulation. However, detection sensitivity is still limited by shortcomings, including photobleaching and weak signal intensities of conventional fluorophores at low concentrations. Plasmonic nanostructures with ultrastrong electromagnetic fields and fluorescence enhancement capability that can overcome these intrinsic defects hold great potential for ultrasensitive bioanalysis...
March 8, 2024: Analytical Chemistry
https://read.qxmd.com/read/38457477/a-novel-method-for-the-isolation-of-single-cells-mimicking-circulating-tumour-cells-adhered-on-smart-bio-surface-slides-by-laser-capture-microdissection
#20
JOURNAL ARTICLE
Grazia Visci, Doron Tolomeo, Angelo Lonoce, Aram Arshadi, Lorenzo Bascetta, Gianluca Trotta, Margot van Riel, Joris Robert Vermeesch, Roberta Carbone, Clelia Tiziana Storlazzi
In recent years, the importance of isolating single cells from blood circulation for several applications, such as non-invasive tumour diagnosis, the monitoring of minimal residual disease, and the analysis of circulating fetal cells for prenatal diagnosis, urged the need to set up innovative methods. For such applications, different methods were developed. All show some weaknesses, especially a limited sensitivity, and specificity. Here we present a new method for isolating a single or a limited number of cells adhered to SBS slides (Tethis S...
2024: PloS One
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