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https://www.readbyqxmd.com/read/28534062/insights-into-the-prevalence-and-underlying-causes-of-clonal-variation-through-transcriptomic-analysis-in-pichia-pastoris
#1
Rochelle Aw, Geraint R Barton, David J Leak
Clonal variation, wherein a range of specific productivities of secreted proteins are observed from supposedly identical transformants, is an accepted aspect of working with Pichia pastoris. It means that a significant number of transformants need to be tested to obtain a representative sample, and in commercial protein production, companies regularly screen thousands of transformants to select for the highest secretor. Here, we have undertaken a detailed investigation of this phenomenon by characterising clones transformed with the human serum albumin gene...
May 22, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28533952/quantifying-climate-change-impacts-emphasises-the-importance-of-managing-regional-threats-in-the-endangered-yellow-eyed-penguin
#2
Thomas Mattern, Stefan Meyer, Ursula Ellenberg, David M Houston, John T Darby, Melanie Young, Yolanda van Heezik, Philip J Seddon
Climate change is a global issue with effects that are difficult to manage at a regional scale. Yet more often than not climate factors are just some of multiple stressors affecting species on a population level. Non-climatic factors-especially those of anthropogenic origins-may play equally important roles with regard to impacts on species and are often more feasible to address. Here we assess the influence of climate change on population trends of the endangered Yellow-eyed penguin (Megadyptes antipodes) over the last 30 years, using a Bayesian model...
2017: PeerJ
https://www.readbyqxmd.com/read/28533753/age-related-assessment-of-sugar-and-protein-intake-of-ceratitis-capitata-in-ad-libitum-conditions-and-modeling-its-relation-to-reproduction
#3
Nikos A Kouloussis, Petros T Damos, Charalambos S Ioannou, Constantinos Tsitsoulas, Nikos T Papadopoulos, David Nestel, Dimitris S Koveos
In the inquiry on the age related dietary assessment of an organism, knowledge of the distributional patterns of food intake throughout the entire life span is very important, however, age related nutritional studies often lack robust feeding quantification methods due to their limitations in obtaining short-term food-intake measurements. In this study, we developed and standardized a capillary method allowing precise life-time measurements of food consumption by individual adult medflies, Ceratitis capitata (Diptera: Tephritidae), under laboratory conditions...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28533623/short-physical-performance-battery-for-middle-aged-and-older-adult-cardiovascular-disease-patients-implication-for-strength-tests-and-lower-extremity-morphological-evaluation
#4
Tomohiro Yasuda, Kazuya Fukumura, Toshiaki Nakajima
[Purpose] To examine if the SPPB is higher with healthy subjects than outpatients, which was higher than inpatients and if the SPPB can be validated assessment tool for strength tests and lower extremity morphological evaluation in cardiovascular disease patients. [Subjects and Methods] Twenty-four middle aged and older adults with cardiovascular disease were recruited from inpatient and outpatient facilities and assigned to separate experimental groups. Twelve age-matched healthy volunteers were assigned to a control group...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28530452/overground-propulsion-kinematics-and-acceleration-in-elite-wheelchair-rugby
#5
David S Haydon, Ross A Pinder, Paul N Grimshaw, William S P Robertson
PURPOSE: Maximal acceleration from standstill has been identified as a key performance indicator in wheelchair rugby; however, the impact of classification and kinematic variables on performance has received limited attention. This study aimed to investigate kinematic variables during maximal acceleration, with level of activity limitation accounted for using sport classification scores. METHODS: 25 elite wheelchair rugby players were analysed in high-, mid-, and low-point groups based on their sporting classification score, which reflects the combined trunk, arm, and hand function, before completing five 5m sprints from a stationary position...
May 22, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/28530215/takotsubo-cardiomyopathy-after-intravenous-epinephrine-administration-following-cardiac-arrest-provoked-by-pneumoperitoneum-a-case-report
#6
Waldemar Elikowski, Malgorzata Małek-Elikowska, Jacek Karoń, Maria Mrozińska, Alina Baszko, Karolina Horbacka
In stress-induced takotsubo cardiomyopathy (TC) high levels of catecholamines, including epinephrine, may be detected in blood. On the other hand, administration of exogenous epinephrine may occasionally result in TC. A CASE REPORT: The authors describe a case of a 58-year-old, otherwise healthy female, with TC which occurred after intravenous injection of 1 mg of epinephrine against cardiac arrest provoked by pneumoperitoneum performed before planned laparoscopic cholecystectomy. She was admitted 3 days earlier due to biliary colic following a dietary mistake...
April 21, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28529528/safety-evaluation-of-potential-toxic-metals-exposure-from-street-foods-consumed-in-mid-west-nigeria
#7
O C Ekhator, N A Udowelle, S Igbiri, R N Asomugha, Z N Igweze, O E Orisakwe
Objective. Street-vended foods offer numerous advantages to food security; nevertheless, the safety of street food should be considered. This study has investigated the level of potential toxic metal (Pb, Cd, Hg, Sb, Mn, and Al) contamination among street-vended foods in Benin City and Umunede. Methods. Twenty street food samples were purchased from vendors at bus stops. Metals were analyzed with atomic absorption spectrophotometry. The methods developed by the US EPA were employed to evaluate the potential health risk of toxic metals...
2017: Journal of Environmental and Public Health
https://www.readbyqxmd.com/read/28528761/responding-to-a-cvdpv1-outbreak-in-ukraine-implications-challenges-and-opportunities
#8
Nino Khetsuriani, Ihor Perehinets, Dorit Nitzan, Dragoslav Popovic, Thomas Moran, Vusala Allahverdiyeva, Shahin Huseynov, Eugene Gavrilin, Liudmyla Slobodianyk, Olha Izhyk, Anna Sukhodolska, Sahar Hegazi, Katerina Bulavinova, Sergei Platov, Patrick O'Connor
BACKGROUND: The European Region, certified polio-free in 2002, remains at risk of wild poliovirus reintroduction and emergence of circulating vaccine-derived polioviruses (cVDPV) until global polio eradication is achieved, as demonstrated by the cVDPV1 outbreak in Ukraine in 2015. METHODS: We reviewed epidemiologic, clinical and virology data on cVDPV cases, surveillance and immunization coverage data, and reports of outbreak-related surveys, country missions, and expert group meetings...
May 18, 2017: Vaccine
https://www.readbyqxmd.com/read/28527796/genetic-parameters-of-mid-infrared-methane-predictions-and-their-relationships-with-milk-production-traits-in-holstein-cattle
#9
P B Kandel, M-L Vanrobays, A Vanlierde, F Dehareng, E Froidmont, N Gengler, H Soyeurt
Many countries have pledged to reduce greenhouse gases. In this context, the dairy sector is one of the identified sectors to adapt production circumstances to address socio-environmental constraints due to its large carbon footprint related to CH4 emission. This study aimed mainly to estimate (1) the genetic parameters of 2 milk mid-infrared-based CH4 proxies [predicted daily CH4 emission (PME, g/d), and log-transformed predicted CH4 intensity (LMI)] and (2) their genetic correlations with milk production traits [milk (MY), fat (FY), and protein (PY) yields] from first- and second-parity Holstein cows...
May 17, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28527795/the-use-of-mid-infrared-spectrometry-to-estimate-the-ration-composition-of-lactating-dairy-cows
#10
M Klaffenböck, A Steinwidder, C Fasching, G Terler, L Gruber, G Mészáros, J Sölkner
The composition of cow milk is strongly affected by the feeding regimen. Because milk components are routinely determined using mid-infrared (MIR) spectrometry, MIR spectra could also be used to estimate an animal's ration composition. The objective of this study was to determine whether and how well amounts of dry matter intake and the proportions of concentrates, hay, grass silage, maize silage, and pasture in the total ration can be estimated using MIR spectra at an individual animal level. A total of 10,200 milk samples and sets of feed intake data were collected from 90 dairy cows at 2 experimental farms of the Agricultural Research and Education Centre in Raumberg-Gumpenstein, Austria...
May 17, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28527530/intergenerational-impact-of-maternal-overnutrition-and-obesity-throughout-pregnancy-in-sheep-on-metabolic-syndrome-in-grandsons-and-granddaughters
#11
C L Pankey, M W Walton, J F Odhiambo, A M Smith, A B Ghnenis, P W Nathanielsz, S P Ford
We previously reported that maternal overnutrition and obesity (MO) throughout pregnancy and lactation in sheep (MOF0) decreases term fetal pancreatic β-cell numbers and increases perirenal adiposity producing hyperphagia, increased adiposity and insulin resistance in adult female offspring (MOF1) fed ad libitum. Pregnant female MOF1 exhibited increased blood glucose from mid to late gestation vs control F1 (CTRF1) though both groups ate only to NRC recommendations. MOF1 ewes delivered female offspring (F2) who like their MOF1 mothers exhibited increased abdominal adiposity and absent neonatal leptin surge...
April 22, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28526286/physical-activity-alters-limb-bone-structure-but-not-entheseal-morphology
#12
Ian J Wallace, Julia M Winchester, Anne Su, Doug M Boyer, Nicolai Konow
Studies of ancient human skeletal remains frequently proceed from the assumption that individuals with robust limb bones and/or rugose, hypertrophic entheses can be inferred to have been highly physically active during life. Here, we experimentally test this assumption by measuring the effects of exercise on limb bone structure and entheseal morphology in turkeys. Growing females were either treated with a treadmill-running regimen for 10 weeks or served as controls. After the experiment, femoral cortical and trabecular bone structure were quantified with μCT in the mid-diaphysis and distal epiphysis, respectively, and entheseal morphology was quantified in the lateral epicondyle...
June 2017: Journal of Human Evolution
https://www.readbyqxmd.com/read/28525618/establishing-expanding-and-certifying-a-closed-colony-of-phlebotomus-argentipes-diptera-psychodidae-for-xenodiagnostic-studies-at-the-kala-azar-medical-research-center-muzaffarpur-bihar-india
#13
Puja Tiwary, Shakti Kumar Singh, Anurag Kumar Kushwaha, Edgar Rowton, David Sacks, Om Prakash Singh, Shyam Sundar, Phillip Lawyer
This pilot project was preliminary and essential to a larger effort to define the ability of certain human-subject groups across the infection spectrum to serve as reservoirs of Leishmania donovani infection to sand flies in areas of anthroponotic transmission such as in Bihar state, India. This is possible only via xenodiagnosis of well-defined subject groups using live vector sand flies. The objective was to establish at the Kala Azar Medical Research Center (KAMRC), Muzaffarpur, Bihar, India, a self-sustaining colony of Phlebotomus argentipes (Annandale & Brunneti), closed to infusion with wild-caught material and certified safe for human xenodiagnosis...
May 19, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28524020/altered-gene-expression-of-epigenetic-modifying-enzymes-in-response-to-dietary-supplementation-with-linseed-oil
#14
Ran Li, Eveline M Ibeagha-Awemu
Recently we showed that 5% linseed oil (LSO) and 5% safflower oil (SFO) supplementation of cow's diets reduced milk fat yield by 30·38 and 32·42% respectively, accompanied by differential expression of genes and regulation by microRNAs (miRNA). This research communication addresses the hypothesis that epigenetic regulation could be involved in the observed milk fat reduction. Thus, this study investigated the gene expression pattern of major epigenetic modifying enzymes in response to dietary supplementation with LSO or SFO...
May 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28521776/solid-medical-waste-a-cross-sectional-study-of-household-disposal-practices-and-reported-harm-in-southern-ghana
#15
Emilia Asuquo Udofia, Gabriel Gulis, Julius Fobil
BACKGROUND: Solid medical waste (SMW) in households is perceived to pose minimal risks to the public compared to SMW generated from healthcare facilities. While waste from healthcare facilities is subject to recommended safety measures to minimize risks to human health and the environment, similar waste in households is often untreated and co-mingled with household waste which ends up in landfills and open dumps in many African countries. In Ghana, the management of this potentially hazardous waste stream at household and community level has not been widely reported...
May 18, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28518067/remote-sensing-evaluation-of-two-spotted-spider-mite-damage-on-greenhouse-cotton
#16
Daniel E Martin, Mohamed A Latheef
The objective of this study was to evaluate a ground-based multispectral optical sensor as a remote sensing tool to assess foliar damage caused by the two-spotted spider mite (TSSM), Tetranychus urticae Koch, on greenhouse grown cotton. TSSM is a polyphagous pest which occurs on a variety of field and horticultural crops. It often becomes an early season pest of cotton in damaging proportions as opposed to being a late season innocuous pest in the mid-southern United States. Evaluation of acaricides is important for maintaining the efficacy of and preventing resistance to the currently available arsenal of chemicals and newly developed control agents...
April 28, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516244/a-comparison-of-basilar-artery-diameters-measured-by-t2wi-and-tof-mr-angiography
#17
Emre Can Çelebioğlu, Muhammed Mustafa Aldur, Selcuk Tunali, Murvet Hayran, Ayşe Beliz Taşçıoğlu, F Mustafa Sargon
PURPOSE: The purpose of our study is to compare basilar artery diameters (BAD) measured by T2WI to diameters measured by TOF MR angiography (MRA). By doing this, we hope to understand how compatible these two methodologies are with each other. METHODS: We used data from 100 patients (59 females, 41 males) who underwent a session of both T2W MRI and TOF MRA at the same time (ages between 18 and 83). We measured BAD by both T2WI and TOF MRA in three different levels...
May 17, 2017: Surgical and Radiologic Anatomy: SRA
https://www.readbyqxmd.com/read/28515028/augmentation-of-the-infraorbital-rim-in-orthognathic-surgery
#18
T Fattahi, S Salman, B Steinberg
Mid-face augmentation via a Le Fort I osteotomy is a commonly performed operation. Advancement of the upper jaw and associated structures (nose, lower cheek areas) can certainly improve function as well as facial aesthetics and harmony. Often, in patients with severe mid-face deficiency, hypoplasia of the maxilla extends all the way up to the infraorbital rims. The receding infraorbital rim contributes to the negative vector of the globes. In patients with this level of mid-face hypoplasia, while advancing the maxilla at the Le Fort I level satisfies all of the requirements for orthognathic surgery, the deficient infraorbital rim remains unchanged and can actually accentuate the negative vector of the globes...
May 14, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28514928/challenges-in-evaluation-of-screening-for-gastric-cancer-among-men-based-on-nonrandomized-design
#19
Ilkka Vohlonen, Matti Härkönen, Nea Malila, Eero Pukkala, Pentti Sipponen, Veli Koistinen, Matti Hakama
BACKGROUND: Objective was to quantify biases in screening for gastric cancer when comparing attenders to nonattenders using serum pepsinogen I (SPGI) level as primary test. METHODS: In mid 1990s, all men aged 51-65 years from two Finnish cities were invited to SPGI screening. Mortality and premature mortality in attenders were compared to nonattenders. Efficacy of screening was studied by 15 years' follow-up of standardized mortality ratio (SMR) and potential years of life lost (PYLL) due to gastric cancer...
July 2017: Acta Oncologica
https://www.readbyqxmd.com/read/28514136/highly-selective-voc-breath-analysis-using-a-broadly-tunable-vecsel
#20
Béla Tuzson, Jana Jágerská, Herbert Looser, Manuel Graf, Ferdinand Felder, Matthias Fill, Luc Tappy, Lukas Emmenegger
A broadly tunable mid-infrared VECSEL is employed in a direct absorption laser spectroscopic setup to measure acetone in breath-like gas matrix. The large wavelength coverage of more than 30 cm(-1) at 3.38 µm allows, besides acetone, the simultaneous measurement of isoprene, ethanol, methanol, methane, and water. Despite the severe spectral interferences from water and alcohols, an unambiguous determination of acetone is realized with a precision of 13 ppbv that is routinely achieved after 5 minutes averaging at typical breath mean acetone levels without any sample preparation...
May 17, 2017: Analytical Chemistry
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