keyword
MENU ▼
Read by QxMD icon Read
search

Raman spectroscopy AND clinical application

keyword
https://www.readbyqxmd.com/read/28649013/online-quantitative-monitoring-of-live-cell-engineered-cartilage-growth-using-diffuse-fiber-optic-raman-spectroscopy
#1
Mads S Bergholt, Michael B Albro, Molly M Stevens
Tissue engineering (TE) has the potential to improve the outcome for patients with osteoarthritis (OA). The successful clinical translation of this technique as part of a therapy requires the ability to measure extracellular matrix (ECM) production of engineered tissues in vitro, in order to ensure quality control and improve the likelihood of tissue survival upon implantation. Conventional techniques for assessing the ECM content of engineered cartilage, such as biochemical assays and histological staining are inherently destructive...
June 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28639667/recent-progress-in-surface-enhanced-raman-spectroscopy-for-biological-and-biomedical-applications-from-cells-to-clinics
#2
REVIEW
D Cialla-May, X-S Zheng, K Weber, J Popp
The application of surface-enhanced Raman spectroscopy (SERS) in biological and biomedical detection schemes is feasible due to its excellent molecular specificity and high sensitivity as well as the capability of SERS to be performed in complex biological compositions. SERS-based investigation of cells, which are the basic structure and functional unit of organisms, represents the starting point of this review. It is demonstrated that SERS provides a deep understanding of living cells as well as their microenvironment which is needed to assess the development of diseases...
June 22, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28635172/doxorubicin-kinetics-and-effects-on-lung-cancer-cell-lines-using-in-vitro-raman-micro-spectroscopy-binding-signatures-drug-resistance-and-dna-repair
#3
Zeineb Farhane, Franck Bonnier, Orla Howe, Alan Casey, Hugh J Byrne
Raman micro-spectroscopy is a non-invasive analytical tool, whose potential in cellular analysis and monitoring drug mechanisms of action has already been demonstrated, and which can potentially be used in pre-clinical and clinical applications for the prediction of chemotherapeutic efficacy. To further investigate such potential clinical application, it is important to demonstrate its capability to differentiate drug mechanisms of action and cellular resistances. Using the example of Doxorubicin (DOX), in this study, it was used to probe the cellular uptake, signatures of chemical binding and subsequent cellular responses, of the chemotherapeutic drug in two lung cancer cell lines, A549 and Calu-1...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28600775/diagnostic-bacteriology-raman-spectroscopy
#4
Rebecca L Pavlicek, Nicole J Crane, Meron Ghebremedhin, Katherine E Cilwa, Eric A Elster
Current clinical methodology for identification of bacterial infections relies predominantly on culturing microbes from patient material and performing biochemical tests. This can often be an inefficient and lengthy process, which has a significant detrimental effect upon patient care. Techniques used in other aspects of molecular research have the potential to revolutionize the way in which diagnostic tests are used and delivered in the clinical setting. The need for rapid, accurate, and cost-effective molecular techniques in the diagnostic laboratory is imperative to improving patient care, preventing the spread of drug resistance and decreasing the overall burden associated with nosocomial infections...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28505414/quantitative-online-liquid-chromatography-surface-enhanced-raman-scattering-lc-sers-of-methotrexate-and-its-major-metabolites
#5
Abdu Subaihi, Drupad K Trivedi, Katherine A Hollywood, James Bluett, Yun Xu, Howbeer Muhamadali, David I Ellis, Royston Goodacre
The application of Raman spectroscopy as a detection method coupled with liquid chromatography (LC) has recently attracted considerable interest, although this has currently been limited to isocratic elution. The combination of LC with rapidly advancing Raman techniques, such as surface-enhanced Raman scattering (SERS), allows for rapid separation, identification and quantification, leading to quantitative discrimination of closely eluting analytes. This study has demonstrated the utility of SERS in conjunction with reversed-phase liquid chromatography (RP-LC), for the detection and quantification of the therapeutically relevant drug molecule methotrexate (MTX) and its metabolites 7-hydroxy methotrexate (7-OH MTX) and 2,4-diamino-N(10)-methylpteroic acid (DAMPA) in pure solutions and mixtures, including spikes into human urine from a healthy individual and patients under medication...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28464521/on-chip-spectroscopic-assessment-of-microbial-susceptibility-to-antibiotics-within-3-5%C3%A2-hours
#6
Ulrich-Christian Schröder, Johanna Kirchhoff, Uwe Hübner, Günter Mayer, Uwe Glaser, Thomas Henkel, Wolfgang Pfister, Wolfgang Fritzsche, Jürgen Popp, Ute Neugebauer
In times of rising antibiotic resistances, there is a high need for fast, sensitive and specific methods to determine antibiotic susceptibilities of bacterial pathogens. Here, we present an integrated microfluidic device in which bacteria from diluted suspensions are captured in well-defined regions using on-chip dielectrophoresis and further analyzed in a label-free and non-destructive manner using Raman spectroscopy. Minimal sample preparation and automated sample processing ensure safe handling of infectious material with minimal hands-on time for the operator...
May 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28440049/analytical-based-methodologies-for-examining-the-in-vitro-absorption-distribution-metabolism-and-elimination-adme-of-silver-nanoparticles
#7
Sesha L A Paluri, John D Ryan, Nhi H Lam, Dhriti Nepal, Ioana E Sizemore
The clinical applications of silver nanoparticles (AgNPs) remain limited due to the lack of well-established methodologies for studying their nanokinetics. Hereby, the primary goal is to adapt a suite of analytical-based methodologies for examining the in vitro absorption, distribution, metabolism, and elimination of AgNPs. Vero 76 and HEK 293 cells are exposed to ≈10-nm spherical AgNPs(+) and AgNPs(-) at relevant concentrations (0-300 µg mL(-1) ) and times (4-48 h). Absorption: Inductively coupled plasma optical emission spectroscopy (ICP-OES) demonstrates that the two AgNP formulations are not bioequivalent...
April 25, 2017: Small
https://www.readbyqxmd.com/read/28405007/rapid-and-accurate-peripheral-nerve-imaging-by-multipoint-raman-spectroscopy
#8
Yasuaki Kumamoto, Yoshinori Harada, Hideo Tanaka, Tetsuro Takamatsu
Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of peripheral nerve fibers is an essential step for Raman spectroscopy towards application in clinical surgery. Here we present a multipoint Raman spectroscopic technique for rapid peripheral nerve imaging. In only 5 seconds, spectra at 32 points situated on ex vivo rat peripheral nerve bundles and adjoining connective tissues were acquired...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28398071/raman-spectroscopy-of-blood-and-blood-components
#9
Chad G Atkins, Kevin Buckley, Michael W Blades, Robin F B Turner
Blood is a bodily fluid that is vital for a number of life functions in animals. To a first approximation, blood is a mildly alkaline aqueous fluid (plasma) in which a large number of free-floating red cells (erythrocytes), white cells (leucocytes), and platelets are suspended. The primary function of blood is to transport oxygen from the lungs to all the cells of the body and move carbon dioxide in the return direction after it is produced by the cells' metabolism. Blood also carries nutrients to the cells and brings waste products to the liver and kidneys...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28388498/raman-spectroscopy-for-the-detection-of-organ-distribution-and-clearance-of-pegylated-reduced-graphene-oxide-and-biological-consequences
#10
Santhakumar Syama, Willi Paul, Arumugam Sabareeswaran, Parayanthala Valappil Mohanan
Graphene, a 2D carbon material has found vast application in biomedical field because of its exciting physico-chemical properties. The large planar sheet like structure helps graphene to act as an effective carrier of drug or biomolecules in enormous amount. However, limited data available on the biocompatibility of graphene upon interaction with the biological system prompts us to evaluate their toxicity in animal model. In this study organ distribution, clearance and toxicity of PEGylated reduced nanographene (PrGO) on Swiss Albino mice was investigated after intraperitoneal and intravenous administration...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28365985/two-dimensional-correlation-spectroscopy-for-multimodal-analysis-of-ft-ir-raman-and-maldi-tof-ms-hyperspectral-images-with-hamster-brain-tissue
#11
Peter Lasch, Isao Noda
Hyperspectral imaging (HSI) techniques are useful for obtaining very detailed structural and compositional information from biomedical, pharmaceutical, or clinical samples, among others. The informative value of these methods can be further increased through the application of different HSI techniques and joint analysis of the data. However, interpretation and understanding of multimodal HSI have been impeded by difficulties in registration of the different HSI data sets and by the lack of integrative analysis concepts...
April 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28340309/analytical-techniques-in-lipidomics-state-of-the-art
#12
Kamil Jurowski, Kamila Kochan, Justyna Walczak, Małgorzata Barańska, Wojciech Piekoszewski, Bogusław Buszewski
Current studies related to lipid identification and determination, or lipidomics in biological samples, are one of the most important issues in modern bioanalytical chemistry. There are many articles dedicated to specific analytical strategies used in lipidomics in various kinds of biological samples. However, in such literature, there is a lack of articles dedicated to a comprehensive review of the actual analytical methodologies used in lipidomics. The aim of this article is to characterize the lipidomics methods used in modern bioanalysis according to the methodological point of view: (1) chromatography/separation methods, (2) spectroscopic methods and (3) mass spectrometry and also hyphenated methods...
March 24, 2017: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/28288455/diagnostic-tools-for-doctors-evaluation-of-tattoo-complications
#13
Jørgen Serup
Diagnosis of tattoo complications is a multi-facetted field since many clinical entities and disease mechanisms are represented. Infections, allergies, and pigment foreign body reactions with granuloma are the major groups. The clinician needs a structured approach to diagnosis and an armamentarium of standard tests. Diagnosis primarily builds on patient history, objective clinical examination, and punch biopsy, supplemented with microbiology testing, ultrasound scanning, and clinical photography. Evaluation of allergic tattoo reactions and allergy to pigments by patch testing is not applicable and has a falsely negative outcome except for the diagnosis of allergy to metals and preservatives...
2017: Current Problems in Dermatology
https://www.readbyqxmd.com/read/28285197/graphene-oxide-functionalized-long-period-grating-for-ultrasensitive-label-free-immunosensing
#14
Chen Liu, Qi Cai, Baojian Xu, Weidong Zhu, Lin Zhang, Jianlong Zhao, Xianfeng Chen
We explore graphene oxide (GO) nanosheets functionalized dual-peak long period grating (dLPG) based biosensor for ultrasensitive label-free antibody-antigen immunosensing. The GO linking layer provides a remarkable analytical platform for bioaffinity binding interface due to its favorable combination of exceptionally high surface-to-volume ratio and excellent optical and biochemical properties. A new GO deposition technique based on chemical-bonding in conjunction with physical-adsorption was proposed to offer the advantages of a strong bonding between GO and fiber device surface and a homogeneous GO overlay with desirable stability, repeatability and durability...
March 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28258037/the-role-of-zinc-deficiency-induced-changes-in-the-phospholipid-protein-balance-of-blood-serum-in-animal-depression-model-by-raman-ftir-and-uv-vis-spectroscopy
#15
J Depciuch, M Sowa-Kućma, G Nowak, B Szewczyk, U Doboszewska, M Parlinska-Wojtan
Depression is a serious mental illness. To study the mechanisms of diseases and search for new, more effective therapies, animal models are used. Unfortunately, none of the available models does reflect all symptoms of depression. Zinc deficiency is proposed as a new animal model of depression. However, it has not been yet validated in a detailed manner. Recently, spectroscopic techniques are increasingly being used both in clinical and preclinical studies. Here we examined the effect of zinc deficiency and amitryptyline treatment on the phospholipid - protein balance in the blood serum of rats using Raman, Fourier Transform Infra Red (FTIR) and UV-vis technique...
May 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28210442/a-portable-fiber-optic-raman-spectrometer-concept-for-evaluation-of-mineral-content-within-enamel-tissue
#16
Anna Akkus, Shan Yang, Renato Roperto, Hathem Mustafa, Sorin Teich, Ozan Akkus
BACKGROUND: Measurement of tooth enamel mineralization using a clinically viable method is essential since variation of mineralization may be used to monitor caries risk or in assessing the effectiveness of remineralization therapy. Fiber optic Raman systems are becoming more affordable and popular in context of biomedical applications. However, the applicability of fiber optic Raman systems for measurement of mineral content within enamel tissue has not been elucidated significantly in the prior literature...
February 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28192951/identification-and-quantification-of-intravenous-therapy-drugs-using-normal-raman-spectroscopy-and-electrochemical-surface-enhanced-raman-spectroscopy
#17
Stephanie Zaleski, Kathleen A Clark, Madison M Smith, Jan Y Eilert, Mark Doty, Richard P Van Duyne
Errors in intravenous (IV) drug therapies can cause human harm and even death. There are limited label-free methods that can sensitively monitor the identity and quantity of the drug being administered. Normal Raman spectroscopy (NRS) provides a modestly sensitive, label-free, and completely noninvasive means of IV drug sensing. In the case that the analyte cannot be detected within its clinical range with Raman, a label-free surface-enhanced Raman spectroscopy (SERS) approach can be implemented to detect the analyte of interest...
January 30, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28042588/review-of-the-potential-of-optical-technologies-for-cancer-diagnosis-in-neurosurgery-a-step-toward-intraoperative-neurophotonics
#18
REVIEW
Fartash Vasefi, Nicholas MacKinnon, Daniel L Farkas, Babak Kateb
Advances in image-guided therapy enable physicians to obtain real-time information on neurological disorders such as brain tumors to improve resection accuracy. Image guidance data include the location, size, shape, type, and extent of tumors. Recent technological advances in neurophotonic engineering have enabled the development of techniques for minimally invasive neurosurgery. Incorporation of these methods in intraoperative imaging decreases surgical procedure time and allows neurosurgeons to find remaining or hidden tumor or epileptic lesions...
January 2017: Neurophotonics
https://www.readbyqxmd.com/read/28027692/comparison-of-3d-printed-poly-%C3%A9-caprolactone-scaffolds-functionalized-with-tricalcium-phosphate-hydroxyapatite-bio-oss-or-decellularized-bone-matrix
#19
Ethan Nyberg, Alexandra Rindone, Amir Dorafshar, Warren L Grayson
Three-dimensional (3D)-printing facilitates rapid, custom manufacturing of bone scaffolds with a wide range of material choices. Recent studies have demonstrated the potential for 3D-printing bioactive (i.e., osteo-inductive) scaffolds for use in bone regeneration applications. In this study, we 3D-printed porous poly-ɛ-caprolactone (PCL) scaffolds using a fused deposition modeling (FDM) process and functionalized them with mineral additives that have been widely used commercially and clinically: tricalcium phosphate (TCP), hydroxyapatite (HA), Bio-Oss (BO), or decellularized bone matrix (DCB)...
February 7, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28001146/development-of-a-high-throughput-ht-raman-spectroscopy-method-for-rapid-screening-of-liquid-blood-plasma-from-prostate-cancer-patients
#20
Dinesh K R Medipally, Adrian Maguire, Jane Bryant, John Armstrong, Mary Dunne, Marie Finn, Fiona M Lyng, Aidan D Meade
Extensive research has been undertaken on the examination of tissue biopsies using vibrational spectroscopic techniques. However, fewer studies have focused on less invasive and commonly acquired blood samples. Recent studies have shown the ability of Raman and Fourier transform infrared (FTIR) spectroscopy to discriminate between non-cancer controls and cancer cases using blood serum or plasma. Even though many studies have proposed Raman spectroscopy as a potential diagnostic tool in various cancers, the Raman spectroscopic technique has not been introduced as a routine clinical technology...
December 21, 2016: Analyst
keyword
keyword
108281
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"