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Raman spectroscopy AND clinical application

Agnieszka Kamińska, Tomasz Szymborski, Tomasz Jaroch, Adam Zmysłowski, Arkadiusz Szterk
Surface-enhanced Raman spectroscopy (SERS) has been widely used in a variety of biomedical, analytical, forensic and environmental investigations due to its chemical specificity, label-free nature combined with high sensitivity. Here, we report a simple method for the fabrication of reproducible and reliable, well-defined, stable SERS substrates with uniform and giant Raman enhancement suitable for routine trace chemical analysis and detection of biological compounds in complex biological fluids. We prepared porous silicone (PS) surface by a galvanostatic anodic etch of crystalline silicon wafers...
March 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Matthew J Baker, Hugh J Byrne, John Chalmers, Peter Gardner, Royston Goodacre, Alex Henderson, Sergei G Kazarian, Francis L Martin, Julian Moger, Nick Stone, Josep Sulé-Suso
Vibrational spectroscopies, based on infrared absorption and/or Raman scattering provide a detailed fingerprint of a material, based on the chemical content. Diagnostic and prognostic tools based on these technologies have the potential to revolutionise our clinical systems leading to improved patient outcome, more efficient public services and significant economic savings. However, despite these strong drivers, there are many fundamental scientific and technological challenges which have limited the implementation of this technology in the clinical arena, although recent years have seen significant progress in addressing these challenges...
March 5, 2018: Analyst
Heng Zhang, Zhenyi Chen, Jiping Wu, Na Chen, Wenjie Xu, Taihao Li, Shupeng Liu
ST36 is a commonly-used acupoint in traditional Chinese medicine (TCM) for treatment of inflammations, pains and gastrointestinal disturbs. For decades,the low power laser acupuncture has been widely applied as an alternative therapy to traditional metal needle acupuncture and achieved relatively fine therapeutic effect for ST36-related symptoms with reduction of uncomfortableness and infection risks. However its disadvantages of low penetrativity and lack of manipulation skills limit its potential performance...
February 15, 2018: Journal of Biophotonics
A Yu Sdobnov, M E Darvin, E A Genina, A N Bashkatov, J Lademann, V V Tuchin
This paper aims to review recent progress in optical clearing of the skin and over naturally turbid biological tissues and blood using this technique in vivo and in vitro with multiphoton microscopy, confocal Raman microscopy, confocal microscopy, NIR spectroscopy, optical coherence tomography, and laser speckle contrast imaging. Basic principles of the technique, its safety, advantages and limitations are discussed. The application of optical clearing agent on a tissue allows for controlling the optical properties of tissue...
February 3, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Padma Malyala, Donatello Laera, Simona Cianetti, Simone Bufali, Marianna Aggravi, Elvira Ianni, Casey Judge, Gillis Otten, Manmohan Singh, Derek T O' Hagan
Adjuvants are necessary to enable vaccine development against a significant number of challenging pathogens for which effective vaccines are not available [1]. We engineered a novel small-molecule immune potentiator (SMIP), a benzonaphthyridine agonist targeting toll-like receptor 7 (TLR7), as a vaccine adjuvant. TLR7 agonist (TLR7a) was engineered to be adsorbed onto aluminum hydroxide (AlOH), and the resulting AlOH/TLR7a was evaluated as a vaccine adjuvant [2-6]. AlOH/TLR7a exploits the flexibility of AlOH formulations, has an application in many vaccine candidates, and induced good efficacy and safety profiles against all tested antigens (bacterial- and viral-derived protein antigens, toxoids, glycoconjugates, etc...
February 5, 2018: Journal of Pharmaceutical Sciences
Anh H Nguyen, Emily A Peters, Zachary D Schultz
Surface enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics, the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the de novo signal from a wide range of biomolecules has been reported to date. In this review, we examine different classes of biomolecules for the signals observed and experimental details that enable their detection...
December 2017: Reviews in Analytical Chemistry
Iwan W Schie, Jan Rüger, Abdullah Saif Mondol, Anuradha Ramoji, Ute Neugebauer, Christoph Krafft, Juergen Popp
We present a high-throughput screening Raman spectroscopy (HTS-RS) platform for a rapid and label-free macromolecular fingerprinting of tens of thousands eukaryotic cells. The newly proposed label-free HTS-RS platform combines automated imaging microscopy with Raman spectroscopy to enable a rapid label-free screening of cells and can be applied to a large number of biomedical and clinical applications. The potential of the new approach is illustrated by two applications: 1. HTS-RS-based differential white blood cell count...
December 29, 2017: Analytical Chemistry
Lana Woolford, Mingzhou Chen, Kishan Dholakia, C Simon Herrington
The ability to provide quantitative, objective and automated pathological analysis would provide enormous benefits for national cancer screening programmes, in terms of both resource reduction and improved patient wellbeing. The move towards molecular pathology through spectroscopic methods shows great promise, but has been restricted by spectral quality, acquisition times, and lack of direct clinical application. In this paper, we present the application of wavelength modulated Raman spectroscopy for the automated label- and fluorescence-free classification of fixed squamous epithelial cells in suspension, such as those produced during a cervical smear test...
December 28, 2017: Journal of Biophotonics
Audrey C Ford, Wan Fung Chui, Anne Y Zeng, Aditya Nandy, Ellen Liebenberg, Carlo Carraro, Galateia Kazakia, Tamara Alliston, Grace D O'Connell
Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis. Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials. However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge. In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication. Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels...
December 9, 2017: Journal of Biomechanics
Rishikesh Pandey, Chi Zhang, Jeon W Kang, Parind M Desai, Ramachandra R Dasari, Ishan Barman, Tulio A Valdez
Otitis media with effusion (OME) is an important and common condition affecting hearing in pediatric patients characterized by the presence of fluid in the middle ear space. The fluid is normally described as serous or mucoid based on differences in the fluid viscosity. The differential diagnosis of two OMEs, namely serous and mucoid is of significant clinical value because while the former is self-limiting, surgical procedure is commonly required for the latter. However, accurate identification of fluid types remains a challenging target unattainable with current clinical modalities due to unavailability of nonperturbative molecular tools...
December 12, 2017: Journal of Biophotonics
Valentina Dinca, Diana Zaharie-Butucel, Luciana Stanica, Simona Brajnicov, Valentina Marascu, Anca Bonciu, Andra Cristocea, Laura Gaman, Mihaela Gheorghiu, Simion Astilean, Alina Vasilescu
Whole cell optical biosensors, made by immobilizing whole algal, bacterial or mammalian cells on various supports have found applications in several fields, from ecology and ecotoxicity testing to biopharmaceutical production or medical diagnostics. We hereby report the deposition of functional bacterial layers of Micrococcus lysodeikticus (ML) via Matrix-Assisted Pulsed Laser Evaporation (MAPLE) on poly(diallyldimethylamonium) (PDDA)-coated-glass slides and their application as an optical biosensor for the detection of lysozyme in serum...
February 1, 2018: Colloids and Surfaces. B, Biointerfaces
Anna C Biermann, Julia Marzi, Eva Brauchle, Maria Schneider, Angela Kornberger, Sherif Abdelaziz, Julian L Wichmann, Christophe T Arendt, Eike Nagel, Kelvin G M Brockbank, Martina Seifert, Katja Schenke-Layland, Ulrich A Stock
OBJECTIVES: Allogeneic frozen cryopreserved heart valves (allografts or homografts) are commonly used in clinical practice. A major obstacle for their application is the limited availability in particular for paediatrics. Allogeneic large animal studies revealed that alternative ice-free cryopreservation (IFC) results in better matrix preservation and reduced immunogenicity. The objective of this study was to evaluate xenogeneic (porcine) compared with allogeneic (ovine) IFC heart valves in a large animal study...
November 27, 2017: European Journal of Cardio-thoracic Surgery
Ingrid Gomes Perez Occhi-Alexandre, Mauro Luciano Baesso, Francielle Sato, Lidiane Vizioli de Castro-Hoshino, Pedro Luiz Rosalen, Raquel Sano Suga Terada, Antonio Medina Neto, Mitsue Fujimaki
BACKGROUND: Photodynamic therapy has a topical action, so it is important for the photosensitizer to penetrate into the tissue to which it has been applied to induce inactivation of the microorganisms. This study aimed to compare the penetration of two different concentrations of erythrosine into sound and in vitro decayed dentin samples. METHODS: This in vitro study evaluated erythrosine (0.3 and 5%) penetration into sound and decayed dentin. A total of 11 dentin discs were prepared and divided into two equal halves, in order to keep one half sound while the other half was submitted to sterilization and an in vitro demineralization model for 5 days...
November 20, 2017: Photodiagnosis and Photodynamic Therapy
Zuzana Chaloupková, Anna Balzerová, Jitka Bařinková, Zdenka Medříková, Pavel Šácha, Petr Beneš, Václav Ranc, Jan Konvalinka, Radek Zbořil
Prostate cancer is one of the most common cancers among men and can in its later stages cause serious medical problems. Due to the limited suitability of current diagnostic biochemical markers, new biomarkers for the detection of prostate cancer are highly sought after. An ideal biomarker should serve as a reliable prognostic marker, be applicable for early diagnosis, and be applicable for monitoring of therapeutic response. One potential candidate is glutamate carboxypeptidase II (GCPII), also known as prostate specific membrane antigen (PSMA), which has a promising role for direct imaging...
January 2, 2018: Analytica Chimica Acta
Z Farhane, H Nawaz, F Bonnier, H J Byrne
This overview groups some of the recent studies highlighting the potential application of Raman micro-spectroscopy as an analytical technique in preclinical development to predict drug mechanism of action and in clinical application as a companion diagnostic and in personalised therapy due to its capacity to predict cellular resistance and therefore to optimise chemotherapeutic treatment efficacy. Notably, the anthracyclines, Doxorubicin and Actinomycin D, elicit similar spectroscopic signatures of subcellular interaction characteristic of the mode of action of intercalation...
October 30, 2017: Journal of Biophotonics
Richard L Evans, Graham A Turner, Susan Bates, Teresa Robinson, David Arnold, Robert E Marriott, Paul D A Pudney, Eleanor Y M Bonnist, Darren Green
The study objectives were to demonstrate that glycerol, when topically applied from a roll-on antiperspirant formulation, can be delivered directly to human skin ex vivo and the axillary stratum corneum (SC) in vivo, and to assess whether it improves the quality of the axillary skin barrier. Ex vivo human skin absorption of glycerol was measured following application of a roll-on antiperspirant formulation containing 4% 13C3-glycerol. Skin distribution of 13C3-glycerol over 24 h was assessed using gas chromatography-mass spectrometry...
November 2017: Archives of Dermatological Research
Jianhua Zhao, Haishan Zeng, Sunil Kalia, Harvey Lui
Raman spectroscopy provides a noninvasive bedside tool that captures unique optical signals via molecular vibrations in tissue samples. Raman theory was discovered at the beginning of the twentieth century, but it was not until the past few decades that it has been used to differentiate skin neoplasms. We provide a brief description of Raman spectroscopy for in vivo skin cancer diagnosis, including the physical principles underlying Raman spectroscopy, its advantages, typical spectra of skin pathologies, and its clinical application for aiding skin cancer diagnosis...
October 2017: Dermatologic Clinics
Alice Gualerzi, Stefania Niada, Chiara Giannasi, Silvia Picciolini, Carlo Morasso, Renzo Vanna, Valeria Rossella, Massimo Masserini, Marzia Bedoni, Fabio Ciceri, Maria Ester Bernardo, Anna Teresa Brini, Furio Gramatica
Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice...
August 29, 2017: Scientific Reports
Andrey Bogomolov, Urszula Zabarylo, Dmitry Kirsanov, Valeria Belikova, Vladimir Ageev, Iskander Usenov, Vladislav Galyanin, Olaf Minet, Tatiana Sakharova, Georgy Danielyan, Elena Feliksberger, Viacheslav Artyushenko
Optical spectroscopy is increasingly used for cancer diagnostics. Tumor detection feasibility in human kidney samples using mid- and near-infrared (NIR) spectroscopy, fluorescence spectroscopy, and Raman spectroscopy has been reported (Artyushenko et al., Spectral fiber sensors for cancer diagnostics in vitro. In Proceedings of the European Conference on Biomedical Optics, Munich, Germany, 21-25 June 2015). In the present work, a simplification of the NIR spectroscopic analysis for cancer diagnostics was studied...
August 19, 2017: Sensors
Binbin Zhou, Shaofei Li, Xianghu Tang, Pan Li, Xiaomin Cao, Borong Yu, Liangbao Yang, Jinhuai Liu
Intramolecular proton transfer of hypoxanthine, induced by application of a laser on the surface of a bare noble nanomaterial, was monitored in real time using surface-enhanced Raman spectroscopy (SERS). This monitoring demonstrated the dependence of the reaction on the identity of the nanomaterial and on the laser power density. The results pave the way for monitoring the proton transfer reaction in various relevant fields. In addition, we observed the presence of the proton transfer phenomenon of hypoxanthine in serum, providing a way to avoid the effect of proton transfer and hence achieve more reliable spectra of sera for clinical diagnosis...
August 31, 2017: Nanoscale
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