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Raman spectroscopy AND clinical application

Abdul K Parchur, Qifei Li, Anhong Zhou
We report the synthesis, characterization, and application of Prussian blue (PB) functionalized CaMoO4:Eu@SiO2@Au nanorod hybrid nanoparticles (HNPs), with multimodal capabilities such as fluorescence imaging, surface-enhanced Raman spectroscopy (SERS) detection and photothermal therapy (PTT). The average size of CaMoO4:Eu@SiO2 NPs was 206 nm. The HNPs are highly dispersible in water for several weeks without settling and show a strong absorption in the near-infrared region, overlapping with the PB absorption between 600 nm and 1000 nm and the surface plasmon resonance of Au nanorods around 800 nm...
October 21, 2016: Biomaterials Science
Kevin M Koo, Eugene J H Wee, Paul N Mainwaring, Yuling Wang, Matt Trau
Cancer is a heterogeneous disease which manifests as different molecular subtypes due to the complex nature of tumor initiation, progression, and metastasis. The concept of precision medicine aims to exploit this cancer heterogeneity by incorporating diagnostic technology to characterize each cancer patient's molecular subtype for tailored treatments. To characterize cancer molecular subtypes accurately, a suite of multiplexed bioassays have currently been developed to detect multiple oncogenic biomarkers. Despite the reliability of current multiplexed detection techniques, novel strategies are still needed to resolve limitations such as long assay time, complex protocols, and difficulty in interpreting broad overlapping spectral peaks of conventional fluorescence readouts...
September 26, 2016: Small
Shuchen Hsieh, Hui-Jye Chen, Shan-Hui Hsu, Yi-Chun Yang, Cheng-Ming Tang, Mei-Yun Chu, Pei-Ying Lin, Ru-Huei Fu, Mei-Lang Kung, Yun-Wen Chen, Bi-Wen Yeh, Huey-Shan Hung
The ideal characteristics of surface modification on the vascular graft for clinical application would be with excellent hemocompatibility, endothelialization capacity, and anti-restenosis ability. Here, Fourier transform infrared spectroscopy (FTIR), surface enhanced raman spectroscopy (SERS), atomic force microscopy (AFM), contact angle () measurement, and thermogravimetric analyzer (TGA) were used to evaluate the chemical and mechanical properties of collagen-gold nanocomposites (collagen+Au) with 17...
October 7, 2016: ACS Applied Materials & Interfaces
Di Yan, Christian Domes, Robert Domes, Timea Frosch, Jürgen Popp, Mathias W Pletz, Torsten Frosch
Fiber enhanced resonance Raman spectroscopy (FERS) is introduced for chemically selective and ultrasensitive analysis of the biomolecules hematin, hemoglobin, biliverdin, and bilirubin. The abilities for analyzing whole intact, oxygenated erythrocytes are proven, demonstrating the potential for the diagnosis of red blood cell related diseases, such as different types of anemia and hemolytic disorders. The optical fiber enables an efficient light-guiding within a miniaturized sample volume of only a few micro-liters and provides a tremendously improved analytical sensitivity (LODs of 0...
October 17, 2016: Analyst
Sixian You, Haohua Tu, Youbo Zhao, Yuan Liu, Eric J Chaney, Marina Marjanovic, Stephen A Boppart
Fatty acids play essential roles in the growth and metastasis of cancer cells. To facilitate their avid growth and proliferation, cancer cells not only alter the fatty acid synthesis and metabolism intracellularly and extracellularly, but also in the macroenvironment via direct or indirect pathways. We report here, using Raman micro-spectroscopy, that an increase in the production of polyunsaturated fatty acids (PUFAs) was identified in both cancerous and normal appearing breast tissue obtained from breast cancer patients and tumor-bearing rats...
2016: Scientific Reports
Zeeshan Sheikh, Abdul Samad Khan, Nima Roohpour, Michael Glogauer, Ihtesham U Rehman
Periodontal disease if left untreated can result in creation of defects within the alveolar ridge. Barrier membranes are frequently used with or without bone replacement graft materials for achieving periodontal guided tissue regeneration (GTR). Surface properties of barrier membranes play a vital role in their functionality and clinical success. In this study polyetherurethane (PEU) membranes were synthesized by using 4,4'-methylene-diphenyl diisocyanate (MDI), polytetramethylene oxide (PTMO) and 1,4-butane diol (BDO) as a chain extender via solution polymerization...
November 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Brandy Broadbent, James Tseng, Rachel Kast, Thomas Noh, Michelle Brusatori, Steven N Kalkanis, Gregory W Auner
Surgical excision of brain tumors provides a means of cytoreduction and diagnosis while minimizing neurologic deficit and improving overall survival. Despite advances in functional and three-dimensional stereotactic navigation and intraoperative magnetic resonance imaging, delineating tissue in real time with physiological confirmation is challenging. Raman spectroscopy is a promising investigative and diagnostic tool for neurosurgery, which provides rapid, non-destructive molecular characterization in vivo or in vitro for biopsy, margin assessment, or laboratory uses...
August 13, 2016: Journal of Neuro-oncology
Eugene J H Wee, Yuling Wang, Simon Chang-Hao Tsao, Matt Trau
Sensitive and accurate identification of specific DNA mutations can influence clinical decisions. However accurate diagnosis from limiting samples such as circulating tumour DNA (ctDNA) is challenging. Current approaches based on fluorescence such as quantitative PCR (qPCR) and more recently, droplet digital PCR (ddPCR) have limitations in multiplex detection, sensitivity and the need for expensive specialized equipment. Herein we describe an assay capitalizing on the multiplexing and sensitivity benefits of surface-enhanced Raman spectroscopy (SERS) with the simplicity of standard PCR to address the limitations of current approaches...
2016: Theranostics
Howbeer Muhamadali, Abdu Subaihi, Mahsa Mohammadtaheri, Yun Xu, David I Ellis, Rajesh Ramanathan, Vipul Bansal, Royston Goodacre
Despite the fact that various microorganisms (e.g., bacteria, fungi, viruses, etc.) have been linked with infectious diseases, their crucial role towards sustaining life on Earth is undeniable. The huge biodiversity, combined with the wide range of biochemical capabilities of these organisms, have always been the driving force behind their large number of current, and, as of yet, undiscovered future applications. The presence of such diversity could be said to expedite the need for the development of rapid, accurate and sensitive techniques which allow for the detection, differentiation, identification and classification of such organisms...
August 15, 2016: Analyst
Felicia S Manciu, John D Ciubuc, Karla Parra, Marian Manciu, Kevin E Bennet, Paloma Valenzuela, Emma M Sundin, William G Durrer, Luis Reza, Giulio Francia
Although not yet ready for clinical application, methods based on Raman spectroscopy have shown significant potential in identifying, characterizing, and discriminating between noncancerous and cancerous specimens. Real-time and accurate medical diagnosis achievable through this vibrational optical method largely benefits from improvements in current technological and software capabilities. Not only is the acquisition of spectral information now possible in milliseconds and analysis of hundreds of thousands of data points achieved in minutes, but Raman spectroscopy also allows simultaneous detection and monitoring of several biological components...
July 4, 2016: Technology in Cancer Research & Treatment
Anne-Isabelle Henry, Bhavya Sharma, M Fernanda Cardinal, Dmitry Kurouski, Richard P Van Duyne
This perspective presents recent developments in the application of surface-enhanced Raman spectroscopy (SERS) to biosensing, with a focus on in vivo diagnostics. We describe the concepts and methodologies developed to date and the target analytes that can be detected. We also discuss how SERS has evolved from a "point-and-shoot" stand-alone technique in an analytical chemistry laboratory to an integrated quantitative analytical tool for multimodal imaging diagnostics. Finally, we offer a guide to the future of SERS in the context of clinical diagnostics...
July 5, 2016: Analytical Chemistry
He Dong, Chuan Liu, Chang-Jian Dai
Aiming at the shortage of the Raman spectra of drugs and the current situation of drug testing, we have applied Raman spectroscopic technique to several kinds of medicine, such as antibiotics, antihistamine, hemocoagulase and antiemetics. The spectral properties for the samples with high Raman activity are investigated by observing their Raman spectra to yield the shift, intensity, and line width of the Raman peaks, as well as the line shape of Raman envelope. For those samples with weak Raman activity or complex structures that are hard to be identified, we have also made some tentative measurements or raise some suggestions for future measurement...
January 2016: Guang Pu Xue Yu Guang Pu Fen Xi, Guang Pu
Arne D Hofemeier, Henning Hachmeister, Christian Pilger, Matthias Schürmann, Johannes F W Greiner, Lena Nolte, Holger Sudhoff, Christian Kaltschmidt, Thomas Huser, Barbara Kaltschmidt
Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches for the detection of osteogenic differentiation are invasive or destructive and not compatible with live cell analysis. Here, non-destructive and label-free approaches of Raman spectroscopy, coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy were used to detect and image osteogenic differentiation of human neural crest-derived inferior turbinate stem cells (ITSCs)...
2016: Scientific Reports
C Fink, H A Haenssle
The diagnosis of advanced cutaneous melanoma may easily be made by the unaided eye, followed by excisional biopsy and histopathological examination. However, in the setting of melanoma screening examinations in high-risk patients with many nevi, dermatologists are challenged with the differentiation of atypical but benign nevi and early invasive or in situ melanomas. In this situation, there is a real need for additional, noninvasive examination techniques that may serve as an aide to decide for or against an excisional biopsy...
July 2016: Der Hautarzt; Zeitschrift Für Dermatologie, Venerologie, und Verwandte Gebiete
Cerys A Jenkins, Paul D Lewis, Peter R Dunstan, Dean A Harris
Colorectal cancer (CRC) is the fourth most common cancer in the United Kingdom and is the second largest cause of cancer related death in the United Kingdom after lung cancer. Currently in the United Kingdom there is not a diagnostic test that has sufficient differentiation between patients with cancer and those without cancer so the current referral system relies on symptomatic presentation in a primary care setting. Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are forms of vibrational spectroscopy that offer a non-destructive method to gain molecular information about biological samples...
May 15, 2016: World Journal of Gastrointestinal Oncology
Anna Akkus, Ozan Akkus, Renato Roperto, Lisa Lang
PURPOSE: To systematically examine mineralisation of healthy human enamel using Raman spectroscopy and provide an understanding of baseline variations that may be inherent in the healthy enamel from individual to individual as well as variations within a tooth. MATERIALS AND METHODS: Human teeth were obtained in compliance with the NIH guidelines. The teeth were collected fresh within the date of the extraction and kept moist at all times with wet tissue paper without any additional disinfecting treatment...
2016: Oral Health & Preventive Dentistry
Stefano Managò, Carmen Valente, Peppino Mirabelli, Diego Circolo, Filomena Basile, Daniela Corda, Anna Chiara De Luca
Acute lymphoblastic leukemia type B (B-ALL) is a neoplastic disorder that shows high mortality rates due to immature lymphocyte B-cell proliferation. B-ALL diagnosis requires identification and classification of the leukemia cells. Here, we demonstrate the use of Raman spectroscopy to discriminate normal lymphocytic B-cells from three different B-leukemia transformed cell lines (i.e., RS4;11, REH, MN60 cells) based on their biochemical features. In combination with immunofluorescence and Western blotting, we show that these Raman markers reflect the relative changes in the potential biological markers from cell surface antigens, cytoplasmic proteins, and DNA content and correlate with the lymphoblastic B-cell maturation/differentiation stages...
2016: Scientific Reports
Zeineb Farhane, Franck Bonnier, Marcus Alexander Maher, Jane Bryant, Alan Casey, Hugh James Byrne
The potential of Raman micro spectroscopy as an in vitro, non-invasive tool for clinical applications has been demonstrated in recent years, specifically for cancer research. To further illustrate its potential as a high content and label free technique, it is important to show its capability to elucidate drug mechanisms of action and cellular resistances. In this study, cytotoxicity assays were employed to establish the toxicity profiles for 24 hr exposure of lung cancer cell lines, A549 and Calu-1, to the commercially available drug, doxorubicin (DOX)...
April 18, 2016: Journal of Biophotonics
Agnieszka Kamińska, Evelin Witkowska, Aneta Kowalska, Anna Skoczyńska, Iwona Gawryszewska, Elżbieta Guziewicz, Dymitr Snigurenko, Jacek Waluk
A highly efficient recognition unit based on surface-enhanced Raman spectroscopy (SERS) was developed as a promising, fast, and sensitive tool for detection of meningococcal meningitis, which is an extremely serious and often fatal disease of the nervous system (an inflammation of the lining around the brain and spinal cord). The results of this study confirmed that there were specific differences in SERS spectra between cerebrospinal fluid (CSF) samples infected by Neisseria meningitidis and the normal CSF, suggesting a potential role for neopterin in meningococcal meningitis detection and screening applications...
June 2016: Analytical and Bioanalytical Chemistry
Zhihao Bai, Guiyin Li, Jingtao Liang, Jing Su, Yue Zhang, Huaizhou Chen, Yong Huang, Weiguo Sui, Yongxiang Zhao
A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction...
August 15, 2016: Biosensors & Bioelectronics
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