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Raman spectroscopy AND clinical application

Fay Nicolson, Lauren E Jamieson, Samuel Mabbott, Konstantinos Plakas, Neil C Shand, Michael R Detty, Duncan Graham, Karen Faulds
In order to improve patient survival and reduce the amount of unnecessary and traumatic biopsies, non-invasive detection of cancerous tumours is of imperative and urgent need. Multicellular tumour spheroids (MTS) can be used as an ex vivo cancer tumour model, to model in vivo nanoparticle (NP) uptake by the enhanced permeability and retention (EPR) effect. Surface enhanced spatially offset Raman spectroscopy (SESORS) combines both surface enhanced Raman spectroscopy (SERS) and spatially offset Raman spectroscopy (SORS) to yield enhanced Raman signals at much greater sub-surface levels...
April 21, 2018: Chemical Science
Ortrud Uckermann, Wenmin Yao, Tareq A Juratli, Roberta Galli, Elke Leipnitz, Matthias Meinhardt, Edmund Koch, Gabriele Schackert, Gerald Steiner, Matthias Kirsch
INTRODUCTION: Mutations in the isocytrate dehydrogenase 1 (IDH1) gene are early genetic events in glioma pathogenesis and cause profound metabolic changes. Because this genotype is found in virtually every tumor cell, therapies targeting mutant IDH1 protein are being developed. The intraoperative administration of those therapies would require fast technologies for the determination of IDH1 genotype. As of today, there is no such diagnostic test available. Recently, infrared spectroscopy was shown to bridge this gap...
May 14, 2018: Journal of Neuro-oncology
Jing Zhang, Victoria Boyes, Frederic Festy, Richard J M Lynch, Timothy F Watson, Avijit Banerjee
OBJECTIVE: To test the null hypothesis that chitosan application has no impact on the remineralisation of artificial incipient enamel white spot lesions (WSLs). METHODS: 66 artificial enamel WSLs were assigned to 6 experimental groups (n=11): (1) bioactive glass slurry, (2) bioactive glass containing polyacrylic acid (BG+PAA) slurry, (3) chitosan pre-treated WSLs with BG slurry (CS-BG), (4) chitosan pre-treated WSLs with BG+PAA slurry (CS-BG+PAA), (5) remineralisation solution (RS) and (6) de-ionised water (negative control, NC)...
May 8, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Fausto Zamparini, Francesco Siboni, Carlo Prati, Paola Taddei, Maria Giovanna Gandolfi
OBJECTIVE: The aim of the study was to evaluate chemical-physical properties and apatite-forming ability of three premixed calcium silicate materials containing monobasic calcium phosphate (CaH4 P2 O8 ) bioceramic, tantalum pentoxide and zirconium oxide, recently marketed for endodontics (TotalFill BC-Sealer, BC-RRM-Paste, BC-RRM-Putty). MATERIALS AND METHODS: Microchemical and micromorphological analyses, radiopacity, initial and final setting times, calcium release and alkalising activity were tested...
May 8, 2018: Clinical Oral Investigations
Hong Wang, Shaohong Zhang, Limei Wan, Hong Sun, Jie Tan, Qiucheng Su
OBJECTIVE: Lung cancer is the leading cause of cancer-related death worldwide. Current clinical screening methods to detect lung cancer are expensive and associated with many complications. Raman spectroscopy is a spectroscopic technique that offers a convenient method to gain molecular information about biological samples. In this study, we measured the serum Raman spectral intensity of healthy volunteers and patients with different stages of non-small cell lung cancer. The purpose of this study was to evaluate the application of serum laser Raman spectroscopy as a low cost alternative method in the screening and staging of non-small cell lung cancer (NSCLC)...
April 13, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Igor V Ermakov, Maia Ermakova, Mohsen Sharifzadeh, Aruna Gorusupudi, Kelliann Farnsworth, Paul S Bernstein, Jodi Stookey, Jane Evans, Tito Arana, Lisa Tao-Lew, Carly Isman, Anna Clayton, Akira Obana, Leah Whigham, Alisha H Redelfs, Lisa Jahns, Werner Gellermann
Resonance Raman spectroscopy (RRS) and reflection spectroscopy (RS) are optical methods applicable to the non-invasive detection of carotenoids in human skin. RRS is the older, more thoroughly validated method, whereas RS is newer and has several advantages. Since collective skin carotenoid levels serve as a biomarker for vegetable and fruit intake, both methods hold promise as convenient screening tools for assessment of dietary interventions and correlations between skin carotenoids and health and disease outcomes...
March 27, 2018: Archives of Biochemistry and Biophysics
Matthew J Baker, Hugh J Byrne, John Chalmers, Peter Gardner, Royston Goodacre, Alex Henderson, Sergei G Kazarian, Francis L Martin, Julian Moger, Nick Stone, Josep Sulé-Suso
Correction for 'Clinical applications of infrared and Raman spectroscopy: state of play and future challenges' by Matthew J. Baker, et al., Analyst, 2018, DOI: 10.1039/c7an01871a.
April 16, 2018: Analyst
Mei Chen, Huilan Su, Li Mao, MingYi Guo, Jiali Tang
This paper describes a voltammetric method for sensitive determination of specific sequences of DNA. The assay is based on three-dimensional nitrogen-doped graphene (3D-NG) which, due to its excellent electrical conductivity, provides a favorable microenvironment to retain the activity of immobilized probe single-stranded DNA and also facilitates electron transfer. The free-standing 3D-NG electrode was characterized by scanning electron microscopy, Raman and X-ray photoelectron spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy...
December 14, 2017: Mikrochimica Acta
Yongqiang Cheng, Lijuan Dong, Jiangyan Zhang, Yaqing Zhao, Zhengping Li
The development of simple, robust, and reliable microRNA (miRNA) detection methods is of great significance in the studies of the biological function of miRNAs, molecular diagnostics, treatment of diseases, and targeted drugs. In recent years, with the increasing development of miRNA research, lots of novel approaches were developed for the detection of miRNA in terms of sensitivity, specificity, multiplicity, in situ imaging, etc. In particular, nucleic acid amplification-based methods and many detection techniques such as droplet digital PCR (ddPCR), electrochemiluminescence (ECL), surface-enhanced Raman spectroscopy (SERS), and mass spectrometry (MS) have been employed widely for the highly sensitive detection of miRNA...
March 21, 2018: Analyst
Xiaozhou Li, Tianyue Yang, Caesar Siqi Li, Youtao Song, Hong Lou, Dagang Guan, Lili Jin
In this paper, we discuss the use of a procedure based on polymerase chain reaction (PCR) and surface enhanced Raman spectroscopy (SERS) (PCR-SERS) to detect DNA mutations. Methods: This method was implemented by first amplifying DNA-containing target mutations, then by annealing probes, and finally by applying SERS detection. The obtained SERS spectra were from a mixture of fluorescence tags labeled to complementary sequences on the mutant DNA. Then, the SERS spectra of multiple tags were decomposed to component tag spectra by multiple linear regression (MLR)...
2018: Theranostics
Chenzhou Wu, John Gleysteen, Nutte Tarn Teraphongphom, Yi Li, Eben Rosenthal
Head and neck cancers become a severe threat to human's health nowadays and represent the sixth most common cancer worldwide. Surgery remains the first-line choice for head and neck cancer patients. Limited resectable tissue mass and complicated anatomy structures in the head and neck region put the surgeons in a dilemma between the extensive resection and a better quality of life for the patients. Early diagnosis and treatment of the pre-malignancies, as well as real-time in vivo detection of surgical margins during en bloc resection, could be leveraged to minimize the resection of normal tissues...
March 18, 2018: International Journal of Oral Science
Agnieszka Kamińska, Tomasz Szymborski, Tomasz Jaroch, Adam Zmysłowski, Arkadiusz Szterk
Surface-enhanced Raman spectroscopy (SERS) has been widely used in a variety of biomedical, analytical, forensic and environmental investigations due to its chemical specificity, label-free nature combined with high sensitivity. Here, we report a simple method for the fabrication of reproducible and reliable, well-defined, stable SERS substrates with uniform and giant Raman enhancement suitable for routine trace chemical analysis and detection of biological compounds in complex biological fluids. We prepared porous silicone (PS) surface by a galvanostatic anodic etch of crystalline silicon wafers...
March 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Matthew J Baker, Hugh J Byrne, John Chalmers, Peter Gardner, Royston Goodacre, Alex Henderson, Sergei G Kazarian, Francis L Martin, Julian Moger, Nick Stone, Josep Sulé-Suso
Vibrational spectroscopies, based on infrared absorption and/or Raman scattering provide a detailed fingerprint of a material, based on the chemical content. Diagnostic and prognostic tools based on these technologies have the potential to revolutionise our clinical systems leading to improved patient outcome, more efficient public services and significant economic savings. However, despite these strong drivers, there are many fundamental scientific and technological challenges which have limited the implementation of this technology in the clinical arena, although recent years have seen significant progress in addressing these challenges...
April 16, 2018: Analyst
Heng Zhang, Zhenyi Chen, Jiping Wu, Na Chen, Wenjie Xu, Taihao Li, Shupeng Liu
ST36 is a commonly-used acupoint in traditional Chinese medicine (TCM) for treatment of inflammations, pains and gastrointestinal disturbs. For decades, the low power laser acupuncture has been widely applied as an alternative therapy to traditional metal needle acupuncture and achieved relatively fine therapeutic effect for ST36-related symptoms with reduction of uncomfortableness and infection risks. However its disadvantages of low penetrativity and lack of manipulation skills limit its potential performance...
February 15, 2018: Journal of Biophotonics
A Yu Sdobnov, M E Darvin, E A Genina, A N Bashkatov, J Lademann, V V Tuchin
This paper aims to review recent progress in optical clearing of the skin and over naturally turbid biological tissues and blood using this technique in vivo and in vitro with multiphoton microscopy, confocal Raman microscopy, confocal microscopy, NIR spectroscopy, optical coherence tomography, and laser speckle contrast imaging. Basic principles of the technique, its safety, advantages and limitations are discussed. The application of optical clearing agent on a tissue allows for controlling the optical properties of tissue...
May 15, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Padma Malyala, Donatello Laera, Simona Cianetti, Simone Bufali, Marianna Aggravi, Elvira Ianni, Casey Judge, Gillis Otten, Manmohan Singh, Derek T O' Hagan
Adjuvants are necessary to enable vaccine development against a significant number of challenging pathogens for which effective vaccines are not available. We engineered a novel small-molecule immune potentiator, a benzonaphthyridine agonist targeting toll-like receptor 7 (TLR7), as a vaccine adjuvant. TLR7 agonist (TLR7a) was engineered to be adsorbed onto aluminum hydroxide (AlOH), and the resulting AlOH/TLR7a was evaluated as a vaccine adjuvant. AlOH/TLR7a exploits the flexibility of AlOH formulations, has an application in many vaccine candidates, and induced good efficacy and safety profiles against all tested antigens (bacterial- and viral-derived protein antigens, toxoids, glycoconjugates, and so forth) in many animal models, including nonhuman primates...
February 6, 2018: Journal of Pharmaceutical Sciences
Anh H Nguyen, Emily A Peters, Zachary D Schultz
Surface enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics, the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the de novo signal from a wide range of biomolecules has been reported to date. In this review, we examine different classes of biomolecules for the signals observed and experimental details that enable their detection...
December 2017: Reviews in Analytical Chemistry
Iwan W Schie, Jan Rüger, Abdullah S Mondol, Anuradha Ramoji, Ute Neugebauer, Christoph Krafft, Jürgen Popp
We present a high-throughput screening Raman spectroscopy (HTS-RS) platform for a rapid and label-free macromolecular fingerprinting of tens of thousands eukaryotic cells. The newly proposed label-free HTS-RS platform combines automated imaging microscopy with Raman spectroscopy to enable a rapid label-free screening of cells and can be applied to a large number of biomedical and clinical applications. The potential of the new approach is illustrated by two applications. (1) HTS-RS-based differential white blood cell count...
February 6, 2018: Analytical Chemistry
Lana Woolford, Mingzhou Chen, Kishan Dholakia, C Simon Herrington
The ability to provide quantitative, objective and automated pathological analysis would provide enormous benefits for national cancer screening programmes, in terms of both resource reduction and improved patient wellbeing. The move towards molecular pathology through spectroscopic methods shows great promise, but has been restricted by spectral quality, acquisition times and lack of direct clinical application. In this paper, we present the application of wavelength modulated Raman spectroscopy for the automated label- and fluorescence-free classification of fixed squamous epithelial cells in suspension, such as those produced during a cervical smear test...
December 28, 2017: Journal of Biophotonics
Audrey C Ford, Wan Fung Chui, Anne Y Zeng, Aditya Nandy, Ellen Liebenberg, Carlo Carraro, Galateia Kazakia, Tamara Alliston, Grace D O'Connell
Native articular cartilage has limited capacity to repair itself from focal defects or osteoarthritis. Tissue engineering has provided a promising biological treatment strategy that is currently being evaluated in clinical trials. However, current approaches in translating these techniques to developing large engineered tissues remains a significant challenge. In this study, we present a method for developing large-scale engineered cartilage surfaces through modular fabrication. Modular Engineered Tissue Surfaces (METS) uses the well-known, but largely under-utilized self-adhesion properties of de novo tissue to create large scaffolds with nutrient channels...
January 23, 2018: Journal of Biomechanics
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