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Emt thyroid

Chenxing Da, Kexia Wu, Chenli Yue, Peisong Bai, Rong Wang, Guanjie Wang, Man Zhao, Yanyan Lv, Peng Hou
Epithelial-mesenchymal transition (EMT), a crucial step in disease progression, plays a key role in tumor metastasis. N-cadherin, a well-known EMT marker, acts as a major oncogene in diverse cancers, whereas its functions in thyroid cancer remains largely unclear. This study was designed to explore the biological roles and related molecular mechanism of N-cadherin in thyroid tumorigenesis. Quantitative RT-PCR (qRT-PCR) and immunohistochemistry assays were used to evaluate N-cadherin expression. A series of in vitro studies such as cell proliferation, colony formation, cell cycle, apoptosis, migration and invasion assays were performed to determine the effect of N-cadherin on malignant behavior of thyroid cancer cells...
December 22, 2016: Oncotarget
Murilo Vieira Geraldo, Helder Imoto Nakaya, Edna Teruko Kimura
Papillary thyroid carcinoma (PTC) is the most prevalent malignant neoplasia of the thyroid gland. A fraction of PTC cases show loss of differentiation and aggressive behavior, with radioiodine therapy resistance and metastasis. Although microRNAs (miRNAs) emerged as promising molecular markers for PTC, their role in the loss of differentiation observed during PTC progression remains to be fully understood. We performed the large-scale analysis of miRNA expression during PTC progression in BRAFT1799A-transgenic animals (Tg-Braf) and thyroid cancer cell lines and identified the marked downregulation of several miRNAs from the region 14q32...
December 24, 2016: Oncotarget
Ying Ni, Spencer Seballos, Benjamin Fletcher, Todd Romigh, Lamis Yehia, Jessica Mester, Leigha Senter, Farshad Niazi, Motoyasu Saji, Matthew D Ringel, Thomas LaFramboise, Charis Eng
Cowden syndrome (CS) is an autosomal dominant disorder that predisposes to breast, thyroid, and other epithelial cancers. Differentiated thyroid carcinoma (DTC), as one of the major component cancers of CS, is the fastest rising incident cancer in the USA, and the most familial of all solid tumors. To identify additional candidate genes of CS and potentially DTC, we analyzed a multi-generation CS-like family with papillary thyroid cancer (PTC), applying a combined linkage-based and whole-genome sequencing strategy and identified an in-frame germline compound heterozygous deletion, p...
December 22, 2016: Human Molecular Genetics
Jiakai Han, Wei Gao, Dongyue Su, Yang Liu
A-kinase anchor protein 4 (AKAP4), a member of the A-kinase anchor proteins, plays a role in tumor development and progression. However, its expression pattern and function in human thyroid cancer remain obscure. Here, we examined AKAP4 expression in thyroid cancer cell lines. Additionally, the effects of AKAP4 on the proliferation and metastasis of thyroid cancer cells was examined. Finally, we explored the molecular mechanism by which AKAP4 mediates the metastatic potential of thyroid cancer cells. Our results revealed that the transcript and protein levels of AKAP4 were significantly up-regulated in thyroid cancer cell lines...
November 8, 2016: Oncology Research
Weixin Liang, Yongqiang Lai, Mingzhang Zhu, Shangshu Huang, Weizhao Feng, Xiaoyu Gu
BACKGROUND Combretastatin A4 (CA4) is a potential therapeutic candidate for a variety of human cancer treatments. However, the inhibitive effects of CA4 on thyroid cancer cells are still not well-clarified. This study aimed to investigate the potential effect of CA4 on thyroid cancer cells, as well as underlying mechanism. MATERIAL AND METHODS Human thyroid papillary carcinoma cell line TPC1 was pre-treated with 5 concentrations of CA4 (0, 1, 2, 5, or 10 μM) for 2 h. Cell proliferation was determined by 3-(4, 5-dimethyl-2- thiazolyl)-2, 5-diphenyl -2-H-tetrazolium bromide (MTT) assay...
December 14, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Hyung Kwon Byeon, Hwi Jung Na, Yeon Ju Yang, Sooah Ko, Sun Och Yoon, Minhee Ku, Jaemoon Yang, Jae Wook Kim, Myung Jin Ban, Ji-Hoon Kim, Da Hee Kim, Jung Min Kim, Eun Chang Choi, Chang-Hoon Kim, Joo-Heon Yoon, Yoon Woo Koh
Previously, the authors have identified that c-Met mediates reactivation of the PI3K/AKT pathway following BRAF inhibitor treatment in BRAF (V600E) mutant anaplastic thyroid cancer, thereby contributing to the acquired drug resistance. Therefore dual inhibition of BRAF and c-Met led to sustained treatment response, thereby maximizing the specific anti-tumor effect of targeted therapy. The present study goes one step further and aims to investigate the effect of acquired resistance of BRAF inhibitor on epithelial-to-mesenchymal transition (EMT) in BRAF mutant thyroid cancer cells and the effect of dual inhibition from combinatorial therapy...
November 21, 2016: Oncotarget
Hai-Wei Zhang, En-Wen Wang, Li-Xian Li, Shou-Hui Yi, Lu-Chun Li, Fa-Liang Xu, Dong-Lin Wang, Yong-Zhong Wu, Wei-Qi Nian
Specificity protein1 (Sp1) is required for TGF-β-induced epithelial-to-mesenchymal transition (EMT) which has been demonstrated to aggravate the progression of cancer including lung cancer. microRNA-29c (miR-29c) is identified to inhibit EMT, but the correlation between miR-29c and Sp1 in human lung cancer remain incompletely clarified. Here, we confirmed decreased expression of miR-29c and enhanced expression of Sp1 in lung cancer tissues (n = 20) and found that Sp1 could be targeted and inhibited by miR-29c...
November 5, 2016: Oncotarget
Fuqiang Wan, Li Peng, ChaoYu Zhu, XinFa Zhang, FangWen Chen, Tao Liu
Latent transforming growth factor (TGF) beta-binding protein 2 (LTBP2) is one of four proteins in the LTBP family of proteins (LTBP-1-4) and was shown to play a vital role in tumorigenesis. However, little is known regarding the functional role of LTBP2 in thyroid carcinoma. Therefore, the current study aimed to evaluate the effect of LTBP2 expression on the proliferation, invasion, and tumorigenesis in thyroid carcinoma cells, and to explore the molecular mechanism of LTBP2 in tumor progression. Our results showed that the expression of LTBP2 is upregulated in human thyroid carcinoma and cell lines...
October 5, 2016: Oncology Research
Ranran Zhang, Heather Hardin, Wei Huang, Jidong Chen, Sofia Asioli, Alberto Righi, Francesca Maletta, Anna Sapino, Ricardo V Lloyd
Long non-coding RNAs (lncRNAs) are important for transcription and for epigenetic or posttranscriptional regulation of gene expression and may contribute to carcinogenesis. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), an lncRNA involved in the regulation of the cell cycle, cell proliferation, and cell migration, is known to be deregulated in multiple cancers. Here, we analyzed the expression of MALAT1 on 195 cases of benign and malignant thyroid neoplasms by using tissue microarrays for RNA in situ hybridization (ISH) and real-time PCR...
September 30, 2016: Endocrine Pathology
Si Li, Hai-Yan Zhang, Zhen-Xian Du, Chao Li, Ming-Xin An, Zhi-Hong Zong, Bao-Qin Liu, Hua-Qin Wang
Beclin 1 has emerged as a haploinsufficient tumor suppression gene in a variety of human carcinomas. In order to clarify the role of Beclin 1 in thyroid cancer, Beclin 1 was knockdown in thyroid cancer cell lines. The current study demonstrated that knockdown of Beclin 1 resulted in morphological and molecular changes of thyroid cancer cells consistent with epithelial-mesenchymal transition (EMT), a morphogenetic procedure during which cells lose their epithelial characteristics and acquire mesenchymal properties concomitantly with gene expression reprogramming...
September 23, 2016: Oncotarget
Jiawei Luo, Wei Huang, Buwen Cao
MicroRNAs (miRNAs) play an essential role in many biological processes by regulating the target genes, especially in the initiation and development of cancers. Therefore, the identification of the miRNA-mRNA regulatory modules is important for understanding the regulatory mechanisms. Most computational methods only used statistical correlations in predicting miRNA-mRNA modules, and neglected the fact there are causal relationships between miRNAs and their target genes. In this paper, we propose a novel approach called CALM(the causal regulatory modules) to identify the miRNA-mRNA regulatory modules through integrating the causal interactions and statistical correlations between the miRNAs and their target genes...
September 21, 2016: IEEE/ACM Transactions on Computational Biology and Bioinformatics
Mingpeng Zhang, Xin Wang, Jin Tan, Minghui Zhao, Linjuan Lian, Weisan Zhang
Poly r(C) binding protein (PCBP) 1 or heterogeneous ribonucleoprotein (hnRNP) E1 is a RNA binding protein functional in multiple biological processes. PCBP1 has been shown to function as a tumor suppressor by negatively regulating translation of EMT inducer proteins in different cancers. Loss of PCBP1 expression or its Akt2-mediated phosphorylation at serine residue 43 has both been indicated to de-repress its regulation of EMT inducer proteins. However, its role in thyroid carcinoma has not been elucidated...
2016: American Journal of Translational Research
Hyeon Jin Kim, Min-Jung Kim, Areumnuri Kim, Chang Won Jung, Sunhoo Park, Jae Soo Koh, Jae Kyung Myung
Purpose: The Notch signaling pathway is widely expressed in normal, reactive, and neoplastic tissues; however, its role in thyroid tissues has not been fully elucidated. Therefore, this study was conducted to characterize the expression of the Notch signaling pathway in papillary thyroid cancer (PTC) cells and anaplastic thyroid cancer (ATC) cells. Materials and Methods: Expression of activated Notch1 in ATC and PTC paraffin-embedded tissues was determined by immunohistochemistry...
September 1, 2016: Cancer Research and Treatment: Official Journal of Korean Cancer Association
Zhaoming Zhong, Zedong Hu, Yue Jiang, Ruimei Sun, Xue Chen, Hongying Chu, Musheng Zeng, Chuanzheng Sun
Metastasis is the major cause of treatment failure in anaplastic thyroid carcinoma (ATC) patients. In the preliminary study, we demonstrated that interleukin (IL)-11 expression is positively correlated with distant metastasis in ATC. However, the mechanisms underlying remain largely unknown. Here, we found that cobalt chloride (a hypoxia mimetic) promoted IL-11 expression via HIF-1α activation. Furthermore, the resultant increase in IL-11 expression significantly induced epithelial-mesenchymal transition (EMT) in ATC cells, accompanied by Akt/GSK3β pathway activation and increased invasive and migratory abilities...
September 13, 2016: Oncotarget
Ana Sastre-Perona, Garcilaso Riesco-Eizaguirre, Miguel A Zaballos, Pilar Santisteban
Mutations in β-catenin are traditionally described as late events in thyroid cancer progression. However, the functional implications of β-catenin dysregulation in the context of tumor initiating events remain unclear. The aim of this work was to investigate whether the two main oncogenic drivers in thyroid cancer, RAS and BRAF, could activate the Wnt/β-catenin pathway. Expression of HRASV12 but not BRAFV600E in thyroid cells induced β-catenin nuclear localization, increased β-catenin-dependent transcriptional activity and inhibited GSK3β...
August 2, 2016: Oncotarget
M Gugnoni, V Sancisi, G Gandolfi, G Manzotti, M Ragazzi, D Giordano, I Tamagnini, M Tigano, A Frasoldati, S Piana, A Ciarrocchi
The transdifferentiation of epithelial cells toward a mesenchymal condition (EMT) is a complex process that allows tumor cells to migrate to ectopic sites. Cadherins are not just structural proteins, but they act as sensors of the surrounding microenvironment and as signaling centers for cellular pathways. However, the molecular mechanisms underlying these signaling functions remain poorly characterized. Cadherin-6 (CDH6) is a type 2 cadherin, which drives EMT during embryonic development and it is aberrantly re-activated in cancer...
July 4, 2016: Oncogene
Marialuisa Sponziello, Francesca Rosignolo, Marilena Celano, Valentina Maggisano, Valeria Pecce, Roberta Francesca De Rose, Giovanni Enrico Lombardo, Cosimo Durante, Sebastiano Filetti, Giuseppe Damante, Diego Russo, Stefania Bulotta
In this study we analyzed the expression levels of markers of epithelial-to-mesenchymal transition (EMT) in several papillary thyroid carcinomas (PTCs) and the relation with tumor genotypes and clinicopathological characteristics. The role of fibronectin-1 (FN1) was investigated by analyzing the effects of FN1 silencing in two human thyroid cancer cell lines. Most of EMT markers were significantly over-expressed in a group of 36 PTCs. In particular, FN1 mRNA levels were higher in tumor vs non-tumor tissue (117...
May 10, 2016: Molecular and Cellular Endocrinology
Brendon Mitchell, Jagdish K Dhingra, Meera Mahalingam
The increased prevalence of BRAF mutations in thyroid carcinoma and primary cutaneous melanoma (PCM) hint that dysregulation of BRAF might contribute to the noted association between PCM and thyroid carcinoma. A recent study evaluating the rate of BRAFV600E mutations among patients who had been diagnosed with primary papillary thyroid carcinoma (PTC) and PCM showed that patients with either PCM or PTC were at an increased risk of developing the other as a second primary malignant neoplasm. Furthermore, the authors noted that samples from patients suffering from both malignancies exhibited a higher rate of incidence of the BRAFV600E mutation, compared with patients not suffering from both malignancies...
July 2016: Advances in Anatomic Pathology
Juliana M Cazarin, Raquel G Coelho, Fabio Hecht, Bruno M Andrade, Denise P Carvalho
BACKGROUND: Differentiated thyroid carcinomas (DTC) are associated with a good prognosis and a high survival rate. However, tumor recurrence occurs in approximately 20-30% of DTC patients, reinforcing the importance of identifying new molecular targets for cancer management. It has been shown that the 5'-AMP-activated protein kinase (AMPK) is over-activated in papillary thyroid cancer (PTC). This study aimed to investigate the effects of 5-aminoimidazole-4-carboxamide-ribonucleoside (AICAR), an AMPK activator, on various aspects of thyroid cancer cell behavior, including cell survival, apoptosis, migration, invasion, and epithelial-to-mesenchymal transition (EMT), in the human thyroid cancer cell lines BCPAP and TPC-1...
July 2016: Thyroid: Official Journal of the American Thyroid Association
Ling Lan, Wei Deng, Hailing Chen, Lili Huo, Lili Deng, Guoying Zhang, Yong Luo
OBJECTIVE: To explore whether nuclear translocation of β-catenin affects functional expression of sodium iodide symporter (NIS) in human thyroid cancer cells. METHODS: Two kinds of β-catenin nuclear translocation cell models were firstly established with transfection of hypoxia inducible factor 1α (HIF-1α) and β-catenin cDNA in human thyroid cancer cell line FTC133. Then the expression of β-catenin were further interfered by shRNA in above two cell models...
March 22, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
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