keyword
MENU ▼
Read by QxMD icon Read
search

mRNA vaccine

keyword
https://www.readbyqxmd.com/read/29022386/bacillus-toyonensis-improves-immune-response-in-the-mice-vaccinated-with-recombinant-antigen-of-bovine-herpesvirus-type-5
#1
F D S Santos, Y A Menegon, R E A Piraine, P R C Rodrigues, R C Cunha, F P Leivas Leite
Probiotics modulate the immune response and can increase the effectiveness of vaccines. Bacillus toyonensis is widely used as a probiotic in animal feed. The aim of this study was to assess the effects of B. toyonensis administration on the immune response to an experimental recombinant vaccine against bovine herpesvirus type 5 (BoHV-5) in mice. Mice were vaccinated with BoHV-5 recombinant glycoprotein D and supplemented with the probiotic B. toyonensis in two regimes: one group received the probiotic only during seven days prior to the initial vaccination while the second group was given the probiotic throughout the experimental period of seven weeks...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/29021398/characterization-of-the-african-swine-fever-virus-decapping-enzyme-during-infection
#2
Ana Quintas, Daniel Pérez-Núñez, Elena G Sánchez, Maria L Nogal, Matthias W Hentze, Alfredo Castelló, Yolanda Revilla
African swine fever virus (ASFV) infection is characterized by a progressive decrease in cellular protein synthesis with a concomitant increase in viral protein synthesis, though the mechanism by which the virus achieves this phenomenon is still unknown. Decrease of cellular mRNA is observed during ASFV infection, suggesting that inhibition of cellular proteins is due to an active mRNA degradation process. ASFV carries a gene (Ba71V D250R/Malawi g5R) that encodes a decapping protein (ASFV-DP) which has a Nudix hydrolase motif and decapping activity in vitro Here we show that ASFV-DP was expressed from early times and accumulated throughout the infection with a subcellular localization typical of the endoplasmic reticulum, co-localizing with the cap structure and interacting with the ribosomal protein L23a...
October 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29018607/effects-of-astragalus-polysaccharide-on-the-adhesion-related-immune-response-of-endothelial-cells-stimulated-with-csfv-in-vitro
#3
Zengyu Zhuge, Yanpeng Dong, Liuan Li, Tianming Jin
BACKGROUND: Astragalus polysaccharide (APS) has immunomodulatory activities on porcine peripheral blood mononuclear cells. The immunomodulatory effects of APS on porcine endothelial cells (ECs) expose to classical swine fever virus (CSFV) remain unknown. METHODS: The virus was titrated using an indirect immune biotin enzyme standard method to confirm that porcine ECs were susceptible to CSFV infection and to determine the TCID50 of CSFV (C-strain). Porcine ECs were cultured with CSFV in the presence of APS...
2017: PeerJ
https://www.readbyqxmd.com/read/28987878/co-delivery-of-nucleoside-modified-mrna-and-tlr-agonists-for-cancer-immunotherapy-restoring-the-immunogenicity-of-immunosilent-mrna
#4
Rein Verbeke, Ine Lentacker, Laura Wayteck, Karine Breckpot, Mieke Van Bockstal, Benedicte Descamps, Christian Vanhove, Stefaan C De Smedt, Heleen Dewitte
This study reports on the design of mRNA and adjuvant-loaded lipid nanoparticles for therapeutic cancer vaccination. The use of nucleoside-modified mRNA has previously been shown to improve the translational capacity and safety of mRNA-therapeutics, as it prevents the induction of type I interferons (IFNs). However, type I IFNs were identified as the key molecules that trigger the activation of antigen presenting cells, and as such drive T cell immunity. We demonstrate that nucleoside-modified mRNA can be co-delivered with the clinically approved TLR agonist monophosphoryl lipid A (MPLA)...
October 5, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28958578/efficient-targeting-and-activation-of-antigen-presenting-cells-in%C3%A2-vivo-after-modified-mrna-vaccine-administration-in-rhesus-macaques
#5
Frank Liang, Gustaf Lindgren, Ang Lin, Elizabeth A Thompson, Sebastian Ols, Josefine Röhss, Shinu John, Kimberly Hassett, Olga Yuzhakov, Kapil Bahl, Luis A Brito, Hugh Salter, Giuseppe Ciaramella, Karin Loré
mRNA vaccines are rapidly emerging as a powerful platform for infectious diseases because they are well tolerated, immunogenic, and scalable and are built on precise but adaptable antigen design. We show that two immunizations of modified non-replicating mRNA encoding influenza H10 hemagglutinin (HA) and encapsulated in lipid nanoparticles (LNP) induce protective HA inhibition titers and H10-specific CD4(+) T cell responses after intramuscular or intradermal delivery in rhesus macaques. Administration of LNP/mRNA induced rapid and local infiltration of neutrophils, monocytes, and dendritic cells (DCs) to the site of administration and the draining lymph nodes (LNs)...
August 12, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28939528/functional-activities-of-interferon-gamma-in-large-yellow-croaker-larimichthys-crocea
#6
Sisi Yang, Qiuhua Li, Yinnan Mu, Jingqun Ao, Xinhua Chen
Interferon gamma (IFN-γ) is a T helper cell type 1 (Th1) cytokine that plays important roles in almost all phases of immune and inflammatory responses. Although IFN-γ gene in large yellow croaker Larimichthys crocea has been reported, little is known about its bioactivity. In this study, large yellow croaker IFN-γ (LycIFN-γ) gene was found to be constitutively expressed in all tissues tested, with the highest levels in blood and heart. Based on stimulation with polyinosinic-polycytidylic acid [poly (I:C)] or inactivated trivalent bacterial vaccine, LycIFN-γ mRNA was significantly increased in spleen and head kidney tissues...
September 20, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28939158/formulation-of-the-bivalent-prostate-cancer-vaccine-with-surgifoam-elicits-antigen-specific-effector-t-cells-in-psa-transgenic-mice
#7
Dev Karan
We previously developed and characterized an adenoviral-based prostate cancer vaccine for simultaneous targeting of prostate-specific antigen (PSA) and prostate stem cell antigen (PSCA). We also demonstrated that immunization of mice with the bivalent vaccine (Ad5-PSA+PSCA) inhibited the growth of established prostate tumors. However, there are multiple challenges hindering the success of immunological therapies in the clinic. One of the prime concerns has been to overcome the immunological tolerance and maintenance of long-term effector T cells...
October 13, 2017: Vaccine
https://www.readbyqxmd.com/read/28935113/directed-evolution-of-glycopeptides-using-mrna-display
#8
Satoru Horiya, Jennifer K Bailey, Isaac J Krauss
Directed evolution is a useful method for the discovery of nucleic acids, peptides, or proteins that have desired binding abilities or functions. Because of the abundance and importance of glycosylation in nature, directed evolution of glycopeptides and glycoproteins is also highly desirable. However, common directed evolution platforms such as phage-, yeast-, or mammalian-cell display are limited for these applications by several factors. Glycan structure at each glycosylation site is not genetically encoded, and yeast and mammalian cells produce a heterogeneous mixture of glycoforms at each site on the protein...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28918045/cationic-nanoliposomes-meet-mrna-efficient-delivery-of-modified-mrna-using-hemocompatible-and-stable-vectors-for-therapeutic-applications
#9
Tatjana Michel, Daniel Luft, Meike-Kristin Abraham, Sabrina Reinhardt, Martha L Salinas Medina, Julia Kurz, Martin Schaller, Meltem Avci-Adali, Christian Schlensak, Karlheinz Peter, Hans Peter Wendel, Xiaowei Wang, Stefanie Krajewski
Synthetically modified mRNA is a unique bioactive agent, ideal for use in therapeutic applications, such as cancer vaccination or treatment of single-gene disorders. In order to facilitate mRNA transfections for future therapeutic applications, there is a need for the delivery system to achieve optimal transfection efficacy, perform with durable stability, and provide drug safety. The objective of our study was to comprehensively analyze the use of 3β-[N-(N',N'-dimethylaminoethane) carbamoyl](DC-Cholesterol)/dioleoylphosphatidylethanolamine (DOPE) liposomes as a potential transfection agent for modified mRNAs...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28916391/zika-virus-structural-biology-and-progress-in-vaccine-development
#10
REVIEW
Hsiao-Han Lin, Bak-Sau Yip, Li-Min Huang, Suh-Chin Wu
The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. High-resolution ZIKV structures recently identified by cryo-electron microscopy indicate that the overall ZIKV structure is similar to those of other flaviviruses...
September 12, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28893281/rna-flow-cytometric-fish-for-investigations-into-hiv-immunology-vaccination-and-cure-strategies
#11
REVIEW
Amy E Baxter, Julia Niessl, Antigoni Morou, Daniel E Kaufmann
Despite the tremendous success of anti-retroviral therapy (ART) no current treatment can eradicate latent HIV reservoirs from HIV-infected individuals or generate, effective HIV-specific immunity. Technological limitations have hampered the identification and characterization of both HIV-infected cells and HIV-specific responses in clinical samples directly ex vivo. RNA-flow cytometric fluorescence in situ hybridisation (RNA Flow-FISH) is a powerful technique, which enables detection of mRNAs in conjunction with proteins at a single-cell level...
September 12, 2017: AIDS Research and Therapy
https://www.readbyqxmd.com/read/28882441/generation-of-a-novel-live-rabies-vaccine-strain-with-a-high-level-of-safety-by-introducing-attenuating-mutations-in-the-nucleoprotein-and-glycoprotein
#12
Keisuke Nakagawa, Kento Nakagawa, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Hiromichi Mitake, Kazuma Okada, Satoko Yamaoka, Yasuhiro Takashima, Tatsunori Masatani, Kota Okadera, Naoto Ito, Tetsuya Mizutani, Makoto Sugiyama
The current live rabies vaccine SAG2 is attenuated by only one mutation (Arg-to-Glu) at position 333 in the glycoprotein (G333). This fact generates a potential risk of the emergence of a pathogenic revertant by a back mutation at this position during viral propagation in the body. To circumvent this risk, it is desirable to generate a live vaccine strain highly and stably attenuated by multiple mutations. However, the information on attenuating mutations other than that at G333 is very limited. We previously reported that amino acids at positions 273 and 394 in the nucleoprotein (N273/394) (Leu and His, respectively) of fixed rabies virus Ni-CE are responsible for the attenuated phenotype by enhancing interferon (IFN)/chemokine gene expressions in infected neural cells...
September 4, 2017: Vaccine
https://www.readbyqxmd.com/read/28881156/nucleic-acid-based-vaccines-targeting-respiratory-syncytial-virus-delivering-the-goods
#13
Trevor R F Smith, Katherine Schultheis, Kate E Broderick
Respiratory syncytial virus (RSV) is a massive medical burden on a global scale. Infants, children and the elderly represent the vulnerable populations. Currently there is no approved vaccine to protect against the disease. Vaccine development has been hindered by several factors including vaccine enhanced disease (VED) associated with formalin-inactivated RSV vaccines, inability of target populations to raise protective immune responses after vaccination or natural viral infection, and a lack of consensus concerning the most appropriate virus-associated target antigen...
September 7, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28878087/novel-stable-ebola-virus-minigenome-replicon-reveals-remarkable-stability-of-the-viral-genome
#14
Wanyin Tao, Tianyu Gan, Mingzhe Guo, Yongfen Xu, Jin Zhong
Ebola virus (EBOV) causes severe hemorrhagic fever in humans and other primates with a high case fatality rate. No approved drug or vaccine of EBOV is available, which necessitates better understanding of the virus life cycle. Studies on EBOV have been hampered because experimentations involving live virus are restricted to biosafety level 4 (BSL-4) laboratories. EBOV minigenome system has provided researchers with the opportunity to study EBOV under BSL-2 conditions. Here, we developed a novel EBOV minigenome replicon which, to our knowledge, is the first EBOV cell culture system that can stably replicate and transcribe EBOV minigenome...
September 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28877647/improvement-of-in-vivo-expression-of-genes-delivered-by-self-amplifying-rna-using-vaccinia-virus-immune-evasion-proteins
#15
Tim Beissert, Lars Koste, Mario Perkovic, Kerstin C Walzer, Stephanie Erbar, Abderaouf Selmi, Mustafa Diken, Sebastian Kreiter, Özlem Türeci, Ugur Sahin
Among nucleic acid based delivery platforms, self-amplifying RNA (saRNA) vectors are of increasing interest for applications such as transient expression of recombinant proteins and vaccination. saRNA is safe and, due to its capability to amplify intracellularly high protein levels, can by produced from even minute amounts of transfected templates. It is an obstacle to full exploitation of this platform, though, that saRNA induces a strong innate host immune response. In transfected cells pattern recognition receptors sense double-stranded RNA intermediates and via activation of protein kinase R (PKR) and interferon signaling initiate host defense measures including a translational shut-down...
September 6, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28855902/different-adjuvants-induce-common-innate-pathways-that-are-associated-with-enhanced-adaptive-responses-against-a-model-antigen-in-humans
#16
Wivine Burny, Andrea Callegaro, Viviane Bechtold, Frédéric Clement, Sophie Delhaye, Laurence Fissette, Michel Janssens, Geert Leroux-Roels, Arnaud Marchant, Robert A van den Berg, Nathalie Garçon, Robbert van der Most, Arnaud M Didierlaurent
To elucidate the role of innate responses in vaccine immunogenicity, we compared early responses to hepatitis B virus (HBV) surface antigen (HBsAg) combined with different Adjuvant Systems (AS) in healthy HBV-naïve adults, and included these parameters in multi-parametric models of adaptive responses. A total of 291 participants aged 18-45 years were randomized 1:1:1:1:1 to receive HBsAg with AS01B, AS01E, AS03, AS04, or Alum/Al(OH)3 at days 0 and 30 (ClinicalTrials.gov: NCT00805389). Blood protein, cellular, and mRNA innate responses were assessed at early time-points and up to 7 days after vaccination, and used with reactogenicity symptoms in linear regression analyses evaluating their correlation with HBs-specific CD4(+) T-cell and antibody responses at day 44...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28843094/mers-coronavirus-nsp1-participates-in-an-efficient-propagation-through-a-specific-interaction-with-viral-rna
#17
Yutaka Terada, Kengo Kawachi, Yoshiharu Matsuura, Wataru Kamitani
MERS-CoV is the only lethal human CoV still endemic in the Arabian Peninsula and neither vaccine nor therapeutics against MERS-CoV infection is available. The nsp1 of CoV is thought to be a major virulence factor because it suppresses protein synthesis through the degradation of host mRNA. In contrast, viral RNA circumvents the nsp1-mediated translational shutoff for an efficient propagation. In this study, we identified amino acid residue in MERS-CoV nsp1 that differ from those of SARS-CoV nsp1, and that appear to be crucial for circumventing the translational shutoff...
November 2017: Virology
https://www.readbyqxmd.com/read/28837660/a-recombinant-multi-antigen-vaccine-with-broad-protection-potential-against-avian-pathogenic-escherichia-coli
#18
Angelica Van Goor, Zachary R Stromberg, Melha Mellata
Chickens are a major source of protein worldwide, yet infectious diseases continue to threaten the poultry industry. Avian pathogenic Escherichia coli (APEC), a subgroup of extraintestinal pathogenic E. coli (ExPEC), causes colibacillosis in chickens resulting in economic loss because of treatment, condemnation of products, and death. In this study, we evaluated a recombinant antigens (rAg) vaccine combining common ExPEC surface proteins EtsC, OmpA, OmpT, and TraT for broad protective potential against APEC infections in chickens...
2017: PloS One
https://www.readbyqxmd.com/read/28830889/dendritic-cell-vaccination-as-postremission-treatment-to-prevent-or-delay-relapse-in-acute-myeloid-leukemia
#19
Sébastien Anguille, Ann L Van de Velde, Evelien L Smits, Viggo F Van Tendeloo, Gunnar Juliusson, Nathalie Cools, Griet Nijs, Barbara Stein, Eva Lion, Ann Van Driessche, Irma Vandenbosch, Anke Verlinden, Alain P Gadisseur, Wilfried A Schroyens, Ludo Muylle, Katrien Vermeulen, Marie-Berthe Maes, Kathleen Deiteren, Ronald Malfait, Emma Gostick, Martin Lammens, Marie M Couttenye, Philippe Jorens, Herman Goossens, David A Price, Kristin Ladell, Yoshihiro Oka, Fumihiro Fujiki, Yusuke Oji, Haruo Sugiyama, Zwi N Berneman
Relapse is a major problem in acute myeloid leukemia (AML) and adversely affects survival. In this phase 2 study, we investigated the effect of vaccination with dendritic cells (DCs) electroporated with Wilms' tumor 1 (WT1) messenger RNA (mRNA) as postremission treatment in 30 patients with AML at very high risk of relapse. There was a demonstrable antileukemic response in 13 patients. Nine patients achieved molecular remission as demonstrated by normalization of WT1 transcript levels, 5 of which were sustained after a median follow-up of 109...
October 12, 2017: Blood
https://www.readbyqxmd.com/read/28826771/astragalus-polysaccharides-enhance-the-immune-response-to-avian-infectious-bronchitis-virus-vaccination-in-chickens
#20
Pengju Zhang, Jun Wang, Weixia Wang, Xiaohui Liu, Haiyan Liu, Xintao Li, Xinghong Wu
Astragalus polysaccharides (APS) are biological macromolecules extracted from Astragalus species that have strong immunoregulatory properties. In this study, APS were employed as an adjuvant for an avian infectious bronchitis virus (IBV) vaccine, and its effects on the cellular immune and humoral immune responses to vaccination in chicken were investigated. One hundred and fifty chicken were randomly divided into five groups (n = 30, each group). The chickens in all groups, except for the unvaccinated control group, were vaccinated with an IBV DNA vaccine...
October 2017: Microbial Pathogenesis
keyword
keyword
108262
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"