keyword
MENU ▼
Read by QxMD icon Read
search

mRNA vaccine

keyword
https://www.readbyqxmd.com/read/28335090/in-ovo-vaccination-using-eimeria-profilin-and-clostridium-perfringens-netb-proteins-in-montanide-ims-adjuvant-increases-protective-immunity-against-experimentally-induced-necrotic-enteritis
#1
Hyun Soon Lillehoj, Seung Ik Jang, Alfredo Panebra, Erik Peter Lillehoj, Laurent Dupuis, Juliette B Arous, Seung Kyoo Lee, Sung Taek Oh
Objective: The effects of vaccinating 18-day-old chicken embryos with the combination of recombinant Eimeria profilin plus Clostridium perfringens NetB proteins mixed in the Montanide IMS adjuvant on the chicken immune response to necrotic enteritis (NE) were investigated using an E. maxima/C. perfringens co-infection NE disease model that we previously developed. Methods: Eighteen-day-old broiler embryos were injected with 100 μl of PBS, profilin, profilin plus NetB, profilin plus NetB/Montanide adjuvant (IMS 106), and profilin plus Net-B/Montanide adjuvant (IMS 101)...
March 21, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28331079/characterization-and-vaccine-potential-of-membrane-vesicles-produced-by-francisella-noatunensis-sup-orientalis-in-an-adult-zebrafish-model
#2
Leidy Lagos, Julia I Tandberg, Urska Repnik, Preben Boysen, Erik Ropstad, Deepa Varkey, Ian T Paulsen, Hanne C Winther-Larsen
Vaccine development against extracellular bacteria has been important for the sustainability of the aquaculture industry. In contrast, infections with intracellular pathogens remain largely an unresolved problem. Francisella noatunensis subspecies orientalis (Fno) are Gram-negative, facultative intracellular bacteria that cause the disease francisellosis in fish. Francisellosis is commonly characterized as a chronic granulomatous disease with high morbidity and can result in high mortality depending on the host...
March 22, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28317871/insights-into-molecular-therapy-of-glioma-current-challenges-and-next-generation-blueprint
#3
REVIEW
Y Rajesh, Ipsita Pal, Payel Banik, Sandipan Chakraborty, Sachin A Borkar, Goutam Dey, Ahona Mukherjee, Mahitosh Mandal
Glioma accounts for the majority of human brain tumors. With prevailing treatment regimens, the patients have poor survival rates. In spite of current development in mainstream glioma therapy, a cure for glioma appears to be out of reach. The infiltrative nature of glioma and acquired resistance substancially restrict the therapeutic options. Better elucidation of the complicated pathobiology of glioma and proteogenomic characterization might eventually open novel avenues for the design of more sophisticated and effective combination regimens...
March 20, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28317440/potent-immune-responses-induced-by-a-salmonella-ghost-delivery-system-that-expresses-the-recombinant-stx2eb-fedf-and-feda-proteins-of-the-escherichia-coli-producing-f18-and-shiga-toxin-in-a-murine-model-and-evaluation-of-its-protective-effect-as-a-porcine-vaccine
#4
Gayeon Won, John Hwa Lee
BACKGROUND: In the pathogenicity of porcine edema disease, which is caused by the Escherichia coli-producing F18 and Shiga toxin, F18(+) fimbrial adhesins and Shiga toxin 2e (Stx2e) play pivotal roles in the colonization and enterotoxicity of this pathogen. OBJECTIVE: To develop a vaccine candidate against edema disease by combining three selected antigens of F18(+) Shiga toxin-producing Escherichia coli (STEC). METHODS: Genetically engineered Salmonella Typhimurium (ST) ghosts that express Stx2eB, FedF, and FedA were individually inserted in a ghost plasmid cassette (pJHL184), and the resultant plasmids were transformed into a Δasd Δlon ΔcpxR ST (JOL912)...
March 20, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28301738/active-immunization-against-transforming-growth-factor-beta1-prevents-hepatic-fibrosis-in-a-rat-model-of-liver-disease
#5
Hong Ji, Gerald Y Minuk, Zhikang Peng, Yongping Chen, Chenwei Pan, Yuewen Gong
Transforming growth factor-beta1 (TGF-β1) plays an important role in hepatic fibrogenesis. To document the effects of active immunization against TGF-β1 on hepatic fibrosis in an animal model of chronic liver disease. BALB/c mice were immunized against three different peptides of TGF-β1 ligated into hepatitis B virus core protein (HBVc). Titers of TGF-β1 antibodies were documented by enzyme linked immunoassays and antibody activity by cell membrane receptor binding and proliferation assays. The most immunogenic recombinant HBVc+TGF-β1 peptide (HBVc+C) then served as a vaccine in Sprague-Dawley rats with dimethylnitrosamine-induced chronic liver disease...
March 16, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28296763/evaluating-the-immunogenicity-and-safety-of-a-biondvax-developed-universal-influenza-vaccine-multimeric-001-either-as-a-standalone-vaccine-or-as-a-primer-to-h5n1-influenza-vaccine-phase-iib-study-protocol
#6
Eva van Doorn, Heng Liu, Tamar Ben-Yedidia, Shimon Hassin, Ildiko Visontai, Stephen Norley, Henderik W Frijlink, Eelko Hak
INTRODUCTION: Influenza is a major respiratory viral infection of humans with high mortality and morbidity rates and profound economic impact. Although influenza vaccines are generally updated yearly to match the viruses expected in the coming season, genetic mutation and reassortment can result in unexpected novel strains. Therefore, it is important to develop universal vaccines inducing protective immunity to such strains before they appear. This clinical trial is designed to evaluate the safety and immunogenicity of Multimeric-001 (M-001), which contains conserved epitopes of influenza A and B...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28289848/genome-wide-associations-of-cd46-and-ifi44l-genetic-variants-with-neutralizing-antibody-response-to-measles-vaccine
#7
Iana H Haralambieva, Inna G Ovsyannikova, Richard B Kennedy, Beth R Larrabee, Michael T Zimmermann, Diane E Grill, Daniel J Schaid, Gregory A Poland
Population-based studies have revealed 2-10% measles vaccine failure rate even after two vaccine doses. While the mechanisms behind this remain unknown, we hypothesized that host genetic factors are likely to be involved. We performed a genome-wide association study of measles specific neutralizing antibody and IFNγ ELISPOT response in a combined sample of 2872 subjects. We identified two distinct chromosome 1 regions (previously associated with MMR-related febrile seizures), associated with vaccine-induced measles neutralizing antibody titers...
April 2017: Human Genetics
https://www.readbyqxmd.com/read/28278192/pbmc-transcriptome-profiles-identifies-potential-candidate-genes-and-functional-networks-controlling-the-innate-and-the-adaptive-immune-response-to-prrsv-vaccine-in-pietrain-pig
#8
Md Aminul Islam, Christine Große-Brinkhaus, Maren Julia Pröll, Muhammad Jasim Uddin, Sharmin Aqter Rony, Dawit Tesfaye, Ernst Tholen, Michael Hoelker, Karl Schellander, Christiane Neuhoff
The porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. A synergistic action of the innate and the adaptive immune system of the host is essential for mounting a durable protective immunity through vaccination. Therefore, the current study aimed to investigate the transcriptome profiles of peripheral blood mononuclear cells (PBMCs) to characterize the innate and the adaptive immune response to PRRS Virus (PRRSV) vaccination in Pietrain pigs...
2017: PloS One
https://www.readbyqxmd.com/read/28277646/efficient-nanovaccine-delivery-in-cancer-immunotherapy
#9
Guizhi Zhu, Fuwu Zhang, Qianqian Ni, Gang Niu, Xiaoyuan Chen
Vaccines hold tremendous potential for cancer immunotherapy by treating the immune system. Subunit vaccines, including molecular adjuvants and cancer-associated antigens or cancer-specific neoantigens, can elicit potent antitumor immunity. However, subunit vaccines have shown limited clinical benefit in cancer patients, which is in part attributed to inefficient vaccine delivery. In this Perspective, we discuss vaccine delivery by synthetic nanoparticles or naturally derived nanoparticles for cancer immunotherapy...
March 9, 2017: ACS Nano
https://www.readbyqxmd.com/read/28277090/serological-response-to-trivalent-inactivated-influenza-vaccine-in-hiv-infected-adults-in-singapore
#10
Yuk-Fai Lau, Lay-Hoon Tang, David Chien Lye, Eng-Eong Ooi, Yee-Sin Leo
A cohort of 81 HIV-infected participants received seasonal trivalent inactivated influenza vaccine (TIV) and their humoral responses were monitored using hemagglutination inhibition (HAI) assay and enzyme-linked immunosorbent assay (ELISA). Three weeks after the vaccination, the percentage of the cohort that had an HAI titer of >1:40 was 35% (for H1N1), 43% (for H3N2) and 19% (for influenza B). An increase in HAI titer can be achieved by an increase in magnitude of the antibody responses, which can be measured by an increase in ELISA titer; as well as a quality improvement of the antibody responses through increased avidity to the virus...
February 17, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28273716/lipid-nanoparticle-assisted-mrna-delivery-for-potent-cancer-immunotherapy
#11
Matthias A Oberli, Andreas M Reichmuth, J Robert Dorkin, Michael J Mitchell, Owen S Fenton, Ana Jaklenec, Daniel G Anderson, Robert Langer, Daniel Blankschtein
The induction of a strong cytotoxic T cell response is an important prerequisite for successful immunotherapy against many viral diseases and tumors. Nucleotide vaccines, including mRNA vaccines with their intracellular antigen synthesis, have been shown to be potent activators of a cytotoxic immune response. The intracellular delivery of mRNA vaccines to the cytosol of antigen presenting immune cells is still not sufficiently well understood. Here, we report on the development of a lipid nanoparticle formulation for the delivery of mRNA vaccines to induce a cytotoxic CD 8 T cell response...
March 8, 2017: Nano Letters
https://www.readbyqxmd.com/read/28254500/molecular-characterization-and-expression-analysis-during-embryo-development-of-cd4-1-homologue-in-large-yellow-croaker-larimichthys-crocea
#12
Kaiqiong Mao, Wei Chen, Yinnan Mu, Jingqun Ao, Xinhua Chen
CD4(+) helper T (Th) cells are a master component of the adaptive immune response. CD4 is one of the most effective surface markers for identifying Th cells. In the present study, we cloned and characterized a CD4-1 homologue, LycCD4-1, from large yellow croaker Larimichthys crocea. The full-length cDNA of LycCD4-1 is 1695 bp long, encoding a protein of 462 amino acids. The deduced LycCD4-1 protein has a typical domain architecture as found in mammalian CD4 molecules, including a signal peptide, four extracellular immunoglobulin-like (Ig-like) domains, a transmembrane region, and a CXC signaling motif in the cytoplasmic tail...
February 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28251619/narcolepsy-patients-blood-based-mirna-expression-profiling-mirna-expression-differences%C3%A2-with-pandemrix-vaccination
#13
N Mosakhani, V Sarhadi, P Panula, M Partinen, S Knuutila
OBJECTIVES: Narcolepsy is a neurological sleep disorder characterized by excessive daytime sleepiness and nighttime sleep disturbance. Among children and adolescents vaccinated with Pandemrix vaccine in Finland and Sweden, the number of narcolepsy cases increased. Our aim was to identify miRNAs involved in narcolepsy and their association with Pandemrix vaccination. MATERIALS AND METHODS: We performed global miRNA proofing by miRNA microarrays followed by RT-PCR verification on 20 narcolepsy patients (Pandemrix-associated and Pandemrix-non-associated) and 17 controls (vaccinated and non-vaccinated)...
March 1, 2017: Acta Neurologica Scandinavica
https://www.readbyqxmd.com/read/28242001/comparative-study-of-the-vaccine-potential-of-six-outer-membrane-proteins-of-edwardsiella-tarda-and-the-immune-responses-of-flounder-paralichthys-olivaceus-after-vaccination
#14
Fuguo Liu, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing, Wenbin Zhan
To obtain effective vaccine candidates for Edwardsiella tarda, six outer membrane proteins (OMPs) of E. tarda were selected and recombinantly expressed. Western blotting analysis showed that flounder anti-E. tarda serum could recognize rOmpV, rOmpK, rOmpX and rOmpI, but not rOmpH and rOmpR. Immunization of flounder with different rOMPs resulted in high levels of protection following the E. tarda challenge with relative percent survivals (RPS) of 92.1% (rOmpI), 84.2% (rOmpX), 68.4% (rOmpK), 60.5% (rOmpV), 57...
March 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28231510/lipopolyplex-potentiates-anti-tumor-immunity-of-mrna-based-vaccination
#15
Stefano Persano, Maria L Guevara, Zhaoqi Li, Junhua Mai, Mauro Ferrari, Pier Paolo Pompa, Haifa Shen
mRNA-based vaccines have the benefit of triggering robust anti-cancer immunity without the potential danger of genome integration from DNA vaccines or the limitation of antigen selection from peptide vaccines. Yet, a conventional mRNA vaccine comprising of condensed mRNA molecules in a positively charged protein core structure is not effectively internalized by the antigen-presenting cells. It cannot offer sufficient protection for mRNA molecules from degradation by plasma and tissue enzymes either. Here, we have developed a lipopolyplex mRNA vaccine that consists of a poly-(β-amino ester) polymer mRNA core encapsulated into a 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine/1,2-dioleoyl-sn-glycero-3-phosphatidyl-ethanolamine/1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000 (EDOPC/DOPE/DSPE-PEG) lipid shell...
May 2017: Biomaterials
https://www.readbyqxmd.com/read/28222903/modified-mrna-vaccines-protect-against-zika-virus-infection
#16
Justin M Richner, Sunny Himansu, Kimberly A Dowd, Scott L Butler, Vanessa Salazar, Julie M Fox, Justin G Julander, William W Tang, Sujan Shresta, Theodore C Pierson, Giuseppe Ciaramella, Michael S Diamond
The emergence of ZIKV infection has prompted a global effort to develop safe and effective vaccines. We engineered a lipid nanoparticle (LNP) encapsulated modified mRNA vaccine encoding wild-type or variant ZIKV structural genes and tested immunogenicity and protection in mice. Two doses of modified mRNA LNPs encoding prM-E genes that produced virus-like particles resulted in high neutralizing antibody titers (∼1/100,000) that protected against ZIKV infection and conferred sterilizing immunity. To offset a theoretical concern of ZIKV vaccines inducing antibodies that cross-react with the related dengue virus (DENV), we designed modified prM-E RNA encoding mutations destroying the conserved fusion-loop epitope in the E protein...
February 16, 2017: Cell
https://www.readbyqxmd.com/read/28217116/phenotypic-consequences-in-vivo-and-in-vitro-of-rearranging-the-p-gene-of-rabv-hep-flury
#17
Mingzhu Mei, Teng Long, Qiong Zhang, Jing Zhao, Qin Tian, Jiaojiao Peng, Jun Luo, Yifei Wang, Yingyi Lin, Xiaofeng Guo
Phosphoprotein (P) of the Rabies virus (RABV) is critically required for viral replication and pathogenicity. Here we manipulated infectious cDNA clones of the RABV HEP-Flury to translocate the P gene from its wild-type position 2 to 1, 3, or 4 in gene order, using an approach which left the viral nucleotide sequence unaltered. The recovered viruses were evaluated for the levels of gene expression, growth kinetics in cell culture, lethality in suckling mice and protection of mice. The results showed that viral replication was affected by the absolute value of N protein which was regulated by P protein...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28197373/systemic-activation-of-antigen-presenting-cells-via-rna-loaded-nanoparticles
#18
Elias J Sayour, Gabriel De Leon, Christina Pham, Adam Grippin, Hanna Kemeny, Joshua Chua, Jianping Huang, John H Sampson, Luis Sanchez-Perez, Catherine Flores, Duane A Mitchell
While RNA-pulsed dendritic cell (DC) vaccines have shown promise, the advancement of cellular therapeutics is fraught with developmental challenges. To circumvent the challenges of cellular immunotherapeutics, we developed clinically translatable nanoliposomes that can be combined with tumor-derived RNA to generate personalized tumor RNA-nanoparticles (NPs) with considerable scale-up capacity. RNA-NPs bypass MHC restriction, are amenable to central distribution, and can provide near immediate immune induction...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28192094/filovirus-proteins-for-antiviral-drug-discovery-structure-function-bases-of-the-replication-cycle
#19
REVIEW
Baptiste Martin, Bruno Canard, Etienne Decroly
Filoviruses are important pathogens that cause severe and often fatal hemorrhagic fever in humans, for which no approved vaccines and antiviral treatments are yet available. In an earlier article (Martin et al., Antiviral Research, 2016), we reviewed the role of the filovirus surface glycoprotein in replication and as a target for drugs and vaccines. In this review, we focus on recent findings on the filovirus replication machinery and how they could be used for the identification of new therapeutic targets and the development of new antiviral compounds...
February 10, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28185789/type-i-interferons-modulate-cd8-t-cell-immunity-to-mrna-vaccines
#20
REVIEW
Ans De Beuckelaer, Johan Grooten, Stefaan De Koker
mRNA vaccines have emerged as potent tools to elicit antitumor T cell immunity. They are characterized by a strong induction of type I interferons (IFNs), potent inflammatory cytokines affecting T cell differentiation and survival. Recent reports have attributed opposing roles for type I IFNs in modulating CD8+ T cell immunity to mRNA vaccines, from profoundly stimulatory to strongly inhibitory. The mechanisms behind this duality are unclear. Disentangling the factors governing the beneficial or detrimental impact of type I IFNs on CD8+ T cell responses is vital to the design of mRNA vaccines of increased potency...
March 2017: Trends in Molecular Medicine
keyword
keyword
108262
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"