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https://www.readbyqxmd.com/read/28230768/bioinformatic-analysis-reveals-archaeal-trna-tyr-and-trna-trp-identities-in-bacteria
#1
Takahito Mukai, Noah M Reynolds, Ana Crnković, Dieter Söll
The tRNA identity elements for some amino acids are distinct between the bacterial and archaeal domains. Searching in recent genomic and metagenomic sequence data, we found some candidate phyla radiation (CPR) bacteria with archaeal tRNA identity for Tyr-tRNA and Trp-tRNA synthesis. These bacteria possess genes for tyrosyl-tRNA synthetase (TyrRS) and tryptophanyl-tRNA synthetase (TrpRS) predicted to be derived from DPANN superphylum archaea, while the cognate tRNA(Tyr) and tRNA(Trp) genes reveal bacterial or archaeal origins...
February 21, 2017: Life
https://www.readbyqxmd.com/read/28228214/inhibition-of-protein-tyrosine-phosphatase-non-receptor-type-2-by-ptp-inhibitor-xix-its-role-as-a-multiphosphatase-inhibitor
#2
Hien Thi Thu Le, Young-Chang Cho, Sayeon Cho
Protein tyrosine phosphatases (PTPs) play crucial roles in signal transduction and their functional alteration has been detected in many diseases. PTP inhibitors have been developed as therapeutic drugs for diseases that are related to the activity of PTPs. In this study, PTP inhibitor XIX, an inhibitor of CD45 and PTEN, was investigated whether it inhibits other PTPs. Protein tyrosine phosphatase non-receptor type 2 (PTPN2) was selectively inhibited by the inhibitor in a competitive manner. Drug affinity responsive target stability (DARTS) analysis showed that the inhibitor induces conformational changes in PTPN2...
February 23, 2017: BMB Reports
https://www.readbyqxmd.com/read/28219781/adenosine-a2-receptor-activation-ameliorates-mitochondrial-oxidative-stress-upon-reperfusion-through-the-posttranslational-modification-of-ndufv2-subunit-of-complex-i-in-the-heart
#3
Jingman Xu, Xiyun Bian, Yuan Liu, Lan Hong, Tianming Teng, Yuemin Sun, Zhelong Xu
While it is well known that adenosine receptor activation protects the heart from ischemia/reperfusion injury, the precise mitochondrial mechanism responsible for the action remains unknown. This study probed the mitochondrial events associated with the cardioprotective effect of 5'-(N-ethylcarboxamido) adenosine (NECA), an adenosine A2 receptor agonist. Isolated rat hearts were subjected to 30min ischemia followed by 10min of reperfusion, whereas H9c2 cells experienced 20min ischemia and 10min reperfusion...
February 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28218795/defensive-coping-and-essential-amino-acid-markers-as-possible-predictors-for-structural-vascular-disease-in-an-african-and-caucasian-male-cohort-the-sabpa-study
#4
Marisa Möller, Leoné Malan, Martin Magnusson, Carina M C Mels, Nico T Malan
Defensive coping (DefS), oxidative stress, inflammation, and related amino acids (phenylalanine [Phe] and tyrosine [Tyr]) have been implicated in cardiovascular disease. This study assessed whether inflammation, oxidative stress, changes in essential amino acids, and altered coping strategies are correlated with subclinical vascular changes in African (n = 82) and Caucasian (n = 100) men from the Sympathetic Activity and Ambulatory Blood Pressure in Africans (SABPA) study. The Coping Strategy Indicator questionnaire identified DefS participants...
February 20, 2017: Psychophysiology
https://www.readbyqxmd.com/read/28216000/pharmacological-and-molecular-characterization-of-the-positive-allosteric-modulators-of-metabotropic-glutamate-receptor-2
#5
REVIEW
L Lundström, C Bissantz, J Beck, M Dellenbach, T J Woltering, J Wichmann, S Gatti
The metabotropic glutamate receptor 2 (mGlu2) plays an important role in the presynaptic control of glutamate release and several mGlu2 positive allosteric modulators (PAMs) have been under assessment for their potential as antipsychotics. The binding mode of mGlu2 PAMs is better characterized in functional terms while few data are available on the relationship between allosteric and orthosteric binding sites. Pharmacological studies characterizing binding and effects of two different chemical series of mGlu2 PAMs are therefore carried out here using the radiolabeled mGlu2 agonist (3)[H]-LY354740 and mGlu2 PAM (3)[H]-2,2,2-TEMPS...
February 16, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28215920/antimicrobial-drug-resistance-and-genetic-properties-of-salmonella-enterica-serotype-enteritidis-circulating-in-chicken-farms-in-tunisia
#6
Rakia Ben Salem, Mohamed S Abbassi, Vanesa García, Raquel García-Fierro, Javier Fernández, Hajer Kilani, Imen Jaouani, Monia Khayeche, Lilia Messadi, María Rosario Rodicio
This study focused on 77 isolates of Salmonella enterica serotype Enteritidis collected during 2009 to 2013 from healthy and sick chickens and environmental farm samples in Tunisia. Resistance to 14 antimicrobials and the encoding genes were analyzed. 66, 26, 6.5, 3.9 and 1.3% were pan-susceptible or showed resistance to nalidixic acid (Asp87 to Tyr and Asp87 to Asn substitutions in GyrA), ampicillin (blaTEM-1-like and blaSHV), sulfonamides (sul1and sul3) and streptomycin (strB), respectively. A single isolate with intermediate susceptibility to ciprofloxacin was positive for qnrB, whereas qnrA, qnrS or aac(6')-Ib-cr were not detected...
February 16, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28215095/ph-dependence-of-photoinduced-electron-transfer-with-ru-tap-3-2
#7
Ludovic Troian-Gautier, Epiphanie Mugeniwabagara, Luca Fusaro, Cécile Moucheron, Andrée Kirsch-De Mesmaeker, Michel Luhmer
The quenching of the excited state of [Ru(TAP)3](2+) (TAP = 1,4,5,8-tetraazaphenanthrene) by guanosine-5'-monophosphate (GMP), N-acetyltyrosine (N-Ac-Tyr), and hydroquinone (H2Q) has been studied in aqueous solution over a wide range of pH values including, for the first time, strongly acidic media. This quenching by electron transfer was examined by steady-state (1)H photochemically induced dynamic nuclear polarization (photo-CIDNP) as well as by more conventional techniques, among which are pulsed laser-induced transient absorption and emission experiments...
February 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28214543/theoretical-structural-characterization-of-lymphoguanylin-a-potential-candidate-for-the-development-of-drugs-to-treat-gastrointestinal-disorders
#8
Állan S Pires, William F Porto, Pryscilla O Castro, Octavio L Franco, Sérgio A Alencar
Guanylin peptides (GPs) are small cysteine-rich peptide hormones involved in salt absorption, regulation of fluids and electrolyte homeostasis. This family presents four members: guanylin (GN), uroguanylin (UGN), lymphoguanylin (LGN) and renoguanylin (RGN). GPs have been used as templates for the development of drugs for the treatment of gastrointestinal disorders. Currently, LGN is the only GP with only one disulfide bridge, making it a remarkable member of this family and a potential drug template; however, there is no structural information about this peptide...
February 16, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28214467/paxillin-a-crossroad-in-pathological-cell-migration
#9
REVIEW
Ana María López-Colomé, Irene Lee-Rivera, Regina Benavides-Hidalgo, Edith López
Paxilllin is a multifunctional and multidomain focal adhesion adapter protein which serves an important scaffolding role at focal adhesions by recruiting structural and signaling molecules involved in cell movement and migration, when phosphorylated on specific Tyr and Ser residues. Upon integrin engagement with extracellular matrix, paxillin is phosphorylated at Tyr31, Tyr118, Ser188, and Ser190, activating numerous signaling cascades which promote cell migration, indicating that the regulation of adhesion dynamics is under the control of a complex display of signaling mechanisms...
February 18, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28212269/separation-and-characterization-of-angiotensin-i-converting-enzyme-ace-inhibitory-peptides-from-saurida-elongata-proteins-hydrolysate-by-imac-ni-2
#10
Lixia Sun, Shanguang Wu, Liqin Zhou, Feng Wang, Xiongdiao Lan, Jianhua Sun, Zhangfa Tong, Dankui Liao
Lizard fish protein hydrolysates (LFPH) were prepared from Lizard fish (Saurida elongata) proteins possessing powerful angiotensin I converting enzyme (ACE) inhibitory activity and the fraction (LFPH-I) with high ACE inhibitory activity was obtained through ultrafiltration. The active Fraction (F2) was isolated from LFPH-I using immobilized metal affinity chromatography (IMAC-Ni(2+)). Analysis of amino acid levels revealed that F2 eluted from IMAC was enriched in Met, His, Tyr, Pro, Ile, and Leu compared to the crude peptide LFPH-I...
February 15, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28202335/purification-and-characterization-of-a-milk-clotting-aspartic-protease-from-withania-coagulans-fruit
#11
Mahmoud Salehi, Mahmoud Reza Aghamaali, Reza H Sajedi, S Mohsen Asghari, Eisa Jorjani
Withania coagulans fruit has traditionally been used as milk coagulant. The present study reports the purification and characterization of an aspartic protease from W. coagulans fruit. The enzyme was purified via fractional ammonium sulfate precipitation and cation exchange chromatography. SDS-PAGE analysis revealed the presence of a monomeric protein with molecular weight of 31kDa. Proteolytic activity (PA) of the protease was evaluated using casein, and the milk-clotting activity (MCA) was analyzed by skim milk...
February 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28194737/regio-selective-intramolecular-hydrogen-deuterium-exchange-in-gas-phase-electron-transfer-dissociation
#12
Yoshitomo Hamuro
Protein backbone amide hydrogen/deuterium exchange mass spectrometry (HDX-MS) typically utilizes enzymatic digestion after the exchange reaction and before MS analysis to improve data resolution. Gas-phase fragmentation of a peptic fragment prior to MS analysis is a promising technique to further increase the resolution. The biggest technical challenge for this method is elimination of intramolecular hydrogen/deuterium exchange (scrambling) in the gas phase. The scrambling obscures the location of deuterium...
February 13, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28192900/biphasic-affinity-chromatographic-approach-for-deep-tyrosine-phosphoproteome-analysis
#13
Zhenzhen Deng, Mingming Dong, Yan Wang, Jing Dong, Shawn S-C Li, Hanfa Zou, Mingliang Ye
Tyrosine phosphorylation (pTyr) is important for normal physiology and implicated in many human diseases, particularly cancer. Identification of pTyr sites is critical to dissecting signaling pathways and understanding disease pathologies. However, compared with serine/threonine phosphorylation (pSer/pThr), the analysis of pTyr at the proteome level is more challenging due to its low abundance. Here, we developed a biphasic affinity chromatographic approach where Src SH2 superbinder was coupled with NeutrAvidin affinity chromatography, for tyrosine phosphoproteome analysis...
February 1, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28190857/protective-effects-of-tyrosol-against-lps-induced-acute-lung-injury-via-inhibiting-nf-%C3%AE%C2%BAb-and-ap-1-activation-and-activating-the-ho-1-nrf2-pathways
#14
Wen-Chen Wang, Yan-Min Xia, Bo Yang, Xiang-Ni Su, Jia-Kuan Chen, Wei Li, Tao Jiang
Tyrosol (Tyr) is a natural antioxidant that displays anti-oxidant and anti-inflammatory properties. The present study aimed to investigate the effect and mechanism of Tyr on lipopolysaccharide (LPS)-induced acute lung injury (ALI). In a mouse model, we found that pretreatment with Tyr significantly improved survival rate, attenuated lung permeability, ameliorated histopathological alterations, reduced expression of the inflammatory mediators and improved expression of the antioxidant enzyme. Further study revealed that Tyr markedly inhibited nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) activation at both in vivo and in vitro levels...
February 10, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28187434/interface-between-breast-cancer-cells-and-the-tumor-microenvironment-using-platelet-rich-plasma-to-promote-tumor-angiogenesis-influence-of-platelets-and-fibrin-bundles-on-the-behavior-of-breast-tumor-cells
#15
Sheila Siqueira Andrade, Joana Tomomi Sumikawa, Eloísa Dognani Castro, Fabricio Pereira Batista, Edgar Paredes-Gamero, Lilian Carolina Oliveira, Izabel Monastério Guerra, Giovani Bravin Peres, Renan Pelluzzi Cavalheiro, Luiz Juliano, Afonso Pinto Nazário, Gil Facina, Siu Mui Tsai, Maria Luiza Vilela Oliva, Manoel João Batista Castello Girão
Cancer progression is associated with an evolving tissue interface of direct epithelial-tumor microenvironment interactions. In biopsies of human breast tumors, extensive alterations in molecular pathways are correlated with cancer staging on both sides of the tumor-stroma interface. These interactions provide a pivotal paracrine signaling to induce malignant phenotype transition, the epithelial-mesenchymal transition (EMT). We explored how the direct contact between platelets-fibrin bundles primes metastasis using platelet-rich plasma (PRP) as a source of growth factors and mimics the provisional fibrin matrix between actively growing breast cancer cells and the tumor stroma...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28187345/protein-tyrosine-nitration-in-plants-present-knowledge-computational-prediction-and-future-perspectives
#16
REVIEW
Zsuzsanna Kolbert, Gábor Feigl, Ádám Bordé, Árpád Molnár, László Erdei
Nitric oxide (NO) and related molecules (reactive nitrogen species) regulate diverse physiological processes mainly through posttranslational modifications such as protein tyrosine nitration (PTN). PTN is a covalent and specific modification of tyrosine (Tyr) residues resulting in altered protein structure and function. In the last decade, great efforts have been made to reveal candidate proteins, target Tyr residues and functional consequences of nitration in plants. This review intends to evaluate the accumulated knowledge about the biochemical mechanism, the structural and functional consequences and the selectivity of plants' protein nitration and also about the decomposition or conversion of nitrated proteins...
February 2, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28186406/substrate-trapping-in-the-siderophore-tailoring-enzyme-pvdq
#17
Kenneth D Clevenger, Romila Mascarenhas, Daniel Catlin, Rui Wu, Neil L Kelleher, Eric J Drake, Andrew M Gulick, Dali Liu, Walter Fast
Siderophore biosynthesis by Pseudomonas aeruginosa enhances virulence and represents an attractive drug target. PvdQ functions in the type-1 pyoverdine biosynthetic pathway by removing a myristoyl anchor from a pyoverdine precursor, allowing eventual release from the periplasm. A circularly permuted version of PvdQ bypasses the self-processing step of this Ntn-hydrolase and retains the activity, selectivity, and structure of wild-type PvdQ, as revealed by a 1.8 Å resolution X-ray crystal structure. A 2.55 Å resolution structure of the inactive S1A/N269D-cpPvdQ mutant in complex with the pyoverdine precursor PVDIq reveals a specific binding pocket for the d-Tyr of this modified peptide substrate...
February 15, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28185437/crosslinked-network-with-rotatable-binding-sites-based-on-mono-carboxylated-%C3%AE-cyclodextrin-2-rotaxane-capable-of-angiotensin-iii-recognition
#18
Ko-Hei Ohmori, Tooru Ooya, Toshifumi Takeuchi
Synthetic receptors selective for target peptides or proteins have received attention because of their potential applications in the separation of biomolecules and biomedical diagnostics. In this study, a [2]rotaxane-based functional monomer containing mono-carboxylated α-cyclodextrin (α-CD) was synthesized, and its crosslinked polymers were evaluated to determine their binding ability to a model peptide, angiotensin III (Arg-Val-Tyr-Ile-His-Pro-Phe), containing an arginine (Arg) residue. The binding ability of the resulting polymers toward angiotensin III, angiotensin IV (Val-Tyr-Ile-His-Pro-Phe), and FMRF-amide (Phe-Met-Arg-Phe) was examined by the batch-binding assay and compared with that of control polymers in which maleic acid-introduced α-CD was chemically crosslinked...
February 10, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28174758/interferon-alpha-promotes-th1-response-and-epithelial-apoptosis-via-inflammasome-activation-in-human-intestinal-mucosa
#19
Anne Jarry, Florent Malard, Chantal Bou-Hanna, Guillaume Meurette, Mohamad Mohty, Jean-François Mosnier, Christian L Laboisse, Céline Bossard
BACKGOUND & AIMS: Several lines of investigation suggest that interferon (IFN) alpha can alter human intestinal mucosa homeostasis. These include the endogenous production of IFN alpha in celiac disease or inflammatory bowel diseases, as well as the occurrence of intestinal side effects of exogenous IFN alpha used as a therapeutic tool. Here, we present an ex vivo translational approach to investigate the effects of IFN alpha on the human normal intestinal mucosa, as well as its underlying mechanisms...
January 2017: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28165370/plumbagin-suppresses-%C3%AE-msh-induced-melanogenesis-in-b16f10-mouse-melanoma-cells-by-inhibiting-tyrosinase-activity
#20
Taek-In Oh, Jeong-Mi Yun, Eun-Ji Park, Young-Seon Kim, Yoon-Mi Lee, Ji-Hong Lim
Recent studies have shown that plumbagin has anti-inflammatory, anti-allergic, antibacterial, and anti-cancer activities; however, it has not yet been shown whether plumbagin suppresses alpha-melanocyte stimulating hormone (α-MSH)-induced melanin synthesis to prevent hyperpigmentation. In this study, we demonstrated that plumbagin significantly suppresses α-MSH-stimulated melanin synthesis in B16F10 mouse melanoma cells. To understand the inhibitory mechanism of plumbagin on melanin synthesis, we performed cellular or cell-free tyrosinase activity assays and analyzed melanogenesis-related gene expression...
February 3, 2017: International Journal of Molecular Sciences
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