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https://www.readbyqxmd.com/read/28343107/phosphorylated-tyrosine-based-pseudobioaffinity-adsorbent-for-the-purification-of-immunoglobulin-g
#1
Gisele Luiza Pavan, Igor Tadeu Lazzarotto Bresolin, Angélica Grespan, Sonia Maria Alves Bueno
The present study evaluated the phosphorylated-tyrosine (P-Tyr) based pseudobioaffinity adsorbent for the purification of human immunoglobulin G (IgG). P-Tyr was selected as a ligand to mimic the natural interactions that occur between the immunoreceptor tyrosine-based activation motif and the IgG. The ligand was coupled to bisoxirane-activated agarose gel and the effect of buffer system, pH, and conductivity was performed to elucidate the nature of IgG-P-Tyr interactions. P-Tyr-agarose was able to purify IgG from human plasma solution in HEPES buffer at pH 7...
March 21, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28342601/ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-ms-e-based-untargeted-milk-metabolomics-in-dairy-cows-with-subclinical-or-clinical-mastitis
#2
Xiaomin Xi, Lai-Yu Kwok, Yuenan Wang, Chen Ma, Zhihui Mi, Heping Zhang
In this study, a novel metabolomics technique based on ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry in the MS(E) mode was used to investigate the milk metabolomics of healthy, subclinical, and clinical mastitis cows, which were classified based on somatic cell count and presentation of clinical symptoms. Meanwhile, univariate and multivariate statistical analyses were performed to identify the significant differences across the 3 groups. Compared with healthy milk samples, less glucose, d-glycerol-1-phosphate, 4-hydroxyphenyllactate, l-carnitine, sn-glycero-3-phosphocholine, citrate, and hippurate were detected in the clinical mastitic milk samples, whereas less d-glycerol-1-phosphate, benzoic acid, l-carnitine, and cis-aconitate were found in the subclinical mastitic milk samples...
March 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28340295/response-of-catalase-of-the-mouse-primary-hepatocytes-to-sodium-dodecylbenzene-sulfonate-and-the-underlying-molecular-mechanisms
#3
Jing Wang, Jiaxi Wang, Lu Zhang, Rutao Liu, Wansong Zong
This study investigated the adverse effects of sodium dodecylbenzene sulfonates (SDBS) on the mouse primary hepatocytes by conducting cell viability, intracellular oxidative stress level and catalase (CAT) activity assays. It was shown that SDBS altered CAT activities, triggered oxidative stress, and thus exhibited cytotoxicity to the hepatocytes. Both the stimulation of intracellular CAT production and the inhibition of molecular CAT activity contributed to intracellular CAT activity change. Molecular mechanisms underlying CAT activity inhibition and structural changes were explored by isothermal titration calorimetry, multi-spectroscopy and molecular docking studies...
March 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28337140/antioxidant-and-anti-tyrosinase-activities-of-phenolic-extracts-from-rape-bee-pollen-and-inhibitory-melanogenesis-by-camp-mitf-tyr-pathway-in-b16-mouse-melanoma-cells
#4
Liping Sun, Yan Guo, Yanxin Zhang, Yongliang Zhuang
Rape bee pollen possesses many nutritional and therapeutic properties because of its abundant nutrimental and bioactive components. In this study, free (FPE) and bound (BPE) phenolic extracts of rape bee pollen were obtained, phenolic and flavonoid contents were determined, and composition of phenolic acids was analyzed. In vitro antioxidant and anti-tyrosinase (TYR) activities of FPE and BPE were compared, and inhibitory melanogenesis of FPE was further evaluated. Results showed FPE and BPE contain total phenolic contents of 11...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28336782/functional-imaging-signature-of-patients-presenting-with-polycythemia-paraganglioma-syndromes
#5
Ingo Janssen, Clara Chi Chen, Zhenping Zhuang, Corina M Millo, Katherine Wolf, Alexander Ling, Frank I Lin, Karen T Adams, Peter Herscovitch, Richard A Feelders, Antonio T Fojo, David Taieb, Electron Kebebew, Karel Pacak
Rationale: Pheochromocytoma/paraganglioma (PPGL) syndromes associated with polycythemia have previously been described in association with mutations in the von Hippel-Lindau (VHL) gene. Recently, mutations in the prolyl hydroxylase gene (PHD) 1 and 2 and in the hypoxia-inducible factor 2 alpha (HIF2A) were also found to be associated with multiple and recurrent PPGL. Such patients also presented with PPGL and polycythemia, and later on, some presented with duodenal somatostatinoma. In additional patients presenting with PPGL and polycythemia, no further mutations have been discovered...
March 23, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28336329/an-aromatic-amino-acid-within-intracellular-loop-2-of-the-prostaglandin-ep2-receptor-is-a-prerequisite-for-selective-association-and-activation-of-g%C3%AE-s
#6
Akiko Yano, Yuko Takahashi, Hiromi Moriguchi, Tomoaki Inazumi, Tomoaki Koga, Akira Otaka, Yukihiko Sugimoto
We previously demonstrated that the aromatic moiety of Tyr(143) within the intracellular loop 2 (ICL2) region of the prostaglandin EP2 receptor plays a crucial role in Gs coupling. Here we investigated whether the ICL2 of the EP2 receptor directly binds to Gαs and whether an aromatic moiety affects this interaction. In Chinese hamster ovary cells, mutations of Tyr(143) reduced the ability of the EP2 receptor to interact with G proteins as demonstrated by GTPγS sensitivity, as well as the ability of agonist-induced cAMP formation, with the rank order of Phe>Tyr (wild-type)=Trp>Leu>Ala (=0)...
March 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28331230/dentin-sialoprotein-facilitates-dental-mesenchymal-cell-differentiation-and-dentin-formation
#7
Wentong Li, Lei Chen, Zhuo Chen, Lian Wu, Junsheng Feng, Feng Wang, Lisa Shoff, Xin Li, Kevin J Donly, Mary MacDougall, Shuo Chen
Dentin sialoprotein (DSP) is a dentin extracellular matrix protein. It is involved in dental mesenchymal cell lineages and dentin formation through regulation of its target gene expression. DSP mutations cause dentin genetic diseases. However, mechanisms of DSP in controlling dental mesenchymal cell differentiation are unknown. Using DSP as bait, we screened a protein library from mouse odontoblastic cells and found that DSP is a ligand and binds to cell surface receptor, occludin. Further study identified that the C-terminal DSP domain(aa 363-458) interacts with the occludin extracellular loop 2(aa 194-241)...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28323554/selective-nitration-of-psbo1-inhibits-oxygen-evolution-from-isolated-arabidopsis-thylakoid-membranes
#8
Misa Takahashi, Jun Shigeto, Atsushi Sakamoto, Hiromichi Morikawa
Treatment of isolated Arabidopsis thaliana thylakoid membranes with nitrogen dioxide (NO2) induces selective nitration of the tyrosine residue at the ninth amino acid ((9)Tyr) of PsbO1. This selective nitration is triggered by light and is inhibited by photosynthetic electron transport inhibitors. Therefore, we postulated that, similar to (161)Tyr of D1 (Yz), (9)Tyr of PsbO1 is redox active and is selectively oxidized by photosynthetic electron transport in response to illumination to a tyrosyl radical that is highly susceptible to nitration...
March 21, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28323037/a-phosphorylation-wide-sncrna-screen-reveals-protein-functional-effector-sncrnas-pfernas-in-human-lung-somatic-cells
#9
Tyler Gable, Yuyan Wang, David Clark, Priti Kumari, Amol Carl Shetty, Li Mao, Yuping Mei
We recently reported that PIWI-interacting RNAs likes (piR-Ls) could regulate functions of the interacting phosphorylated proteins (p-Proteins). In addition, except for writers and erasers, functional efficacy of p-Proteins on their readers still remains unknown. We, therefore, reasoned there was a type of sncRNAs which could regulate functional efficacy of p-Proteins. Here, we profiled sncRNAs interacting with phosphorylated -Ser, -Thr and -Tyr residues in 3 HBE and 4 lung SCC cell lines, investigated effects and mechanisms of phosphorylated-residue-interacting sncRNAs...
March 16, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28322027/rapid-differentiating-colorectal-cancer-and-colorectal-polyp-using-dried-blood-spot-mass-spectrometry-metabolomic-approach
#10
Yu Jing, Xue Wu, Peng Gao, Zhongze Fang, Jingjing Wu, Qingjun Wang, Chen Li, Zhitu Zhu, Yunfeng Cao
Colorectal cancer (CRC) is the third leading causes of cancer mortality, and the early-stage detection could significantly enhance survival rates. Cancer influences the important metabolic pathways and the changes in metabolite levels had been used in many studies as the potential biomarkers. This study is aimed at screening metabolite biomarkers with CRC diagnosis potentials. The direct infusion mass spectrometry (MS) metabolomic analysis based on dried blood spot was used to distinguish CRC from polyp. The target metabolites were composed of 23 amino acids and 26 acylcarnitines...
March 20, 2017: IUBMB Life
https://www.readbyqxmd.com/read/28321929/the-effect-of-butin-on-the-vitiligo-mouse-model-induced-by-hydroquinone
#11
Shi-Xia Huo, Qiong Wang, Xin-Min Liu, Chun-Hui Ge, Li Gao, Xiao-Ming Peng, Ming Yan
Vernonia anthelmintica (L.) Willd has been traditionally used in the treatment of vitiligo in Uyghur medicine. This study used butin, the main component of V. anthelmintica, to study the influence on hydroquinone-induced vitiligo in mice. The animals were randomly divided into six groups: control, model, 8-methoxypsoralen (8-MOP, 4.25 mg/kg), and butin (0.425, 4.25, and 42.5 mg/kg) groups. The number of melanin-containing hair follicles, basal layer melanocytes, melanin-containing epidermal cells, the expression of tyrosinase (TYR) and tyrosinase-related protein-1 (TRP-1), the malondialdehyde (MDA), and cholinesterase (CHE) activity in serum were measured...
March 21, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28320783/pitfalls-in-the-response-evaluation-after-prrt-with-177-lu-dota-0-tyr-3-octreotate
#12
Tessa Brabander, Wouter Van der Zwan, Jaap J M Teunissen, Boen L R Kam, Wouter W de Herder, R A Feelders, Eric P Krenning, Dik Kwekkeboom
Peptide receptor radionuclide therapy (PRRT) with [177Lu-DOTA0,Tyr3]octreotate (177Lu-DOTATATE) is a treatment with good results in patients with metastatic gastroenteropancreatic neuroendocrine tumour (GEPNETs). However, there are some pitfalls that should be taken into consideration when evaluating the treatment response after PRRT. 354 Dutch patients with GEPNETs who were treated with 177Lu-DOTATATE between March 2000 and December 2011 were retrospectively selected. Liver function parameters and chromogranin A were measured before each therapy and in follow-up...
March 20, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/28319020/in%C3%A2-vitro-preparation-and-assessment-of-radical-reducing-peptide-from-octopus-aegina-using-digestive-proteases
#13
Sekar Sudhakar, Rasool Abdul Nazeer
Antioxidant peptides protect biological macromolecules against radical damages. The use of these peptides was evaluated using free radicals scavenging assays [2,2-diphenyl-1 picrylhydrazyl (DPPH) and hydroxyl] with the help of UV-visible and electron spin resonance (ESR) spectroscopy methods. The Octopus aegina mantle protein were tested upon hydrolysis using gastrointestinal enzymes up to 12 h, where pepsin hydrolysate exhibited superior properties (DPPH: 44.39±0.67% and hydroxyl: 38.84±1.07%) compared with trypsin and α-chymotrypsin...
March 16, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28318893/design-and-characterization-of-opioid-ligands-based-on-cycle-in-macrocycle-scaffold
#14
Anna Adamska-Bartłomiejczyk, Rossella De Marco, Luca Gentilucci, Alicja Kluczyk, Anna Janecka
The study reports on a series of novel cyclopeptides based on the structure Tyr-[d-Lys-Phe-Phe-Asp]NH2, a mixed mu and kappa opioid receptor agonist with low nanomolar affinity, in which Phe(4) residue was substituted by cyclic amino acids, such as Pro or its six-membered surrogates, piperidine-2-, 3- or 4-carboxylic acids (Pip, Nip and Inp, respectively). All derivatives exhibited high mu- and moderate delta-opioid receptor affinity, and almost no binding to the kappa-opioid receptor. Conformational analysis suggested that the cis conformation of the peptide bond Phe(3)-Xaa(4) influences receptor selectivity through the control of the position of Phe(3) side chain...
February 28, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28318885/genetic-association-of-the-functional-cdhr3-genotype-with-early-onset-adult-asthma-in-japanese-populations
#15
Jun Kanazawa, Hironori Masuko, Yohei Yatagai, Tohru Sakamoto, Hideyasu Yamada, Yoshiko Kaneko, Haruna Kitazawa, Hiroaki Iijima, Takashi Naito, Takefumi Saito, Emiko Noguchi, Satoshi Konno, Masaharu Nishimura, Tomomitsu Hirota, Mayumi Tamari, Nobuyuki Hizawa
BACKGROUND: Recent studies have demonstrated that a coding SNP (rs6967330, Cys529→Tyr) in cadherin-related family member 3 (CDHR3), which was previously associated with wheezing illness and hospitalizations in infancy, could support efficient human rhinovirus C (RV-C) entry and replication. Here, we sought to examine the genetic contribution of this variant to the development of adult asthma. METHODS: We performed a candidate gene case-control association study of 2 independent Japanese populations (a total of 3366 adults)...
March 16, 2017: Allergology International: Official Journal of the Japanese Society of Allergology
https://www.readbyqxmd.com/read/28316028/improved-positron-emission-tomography-imaging-of-glioblastoma-cancer-using-novel-68-ga-labeled-peptides-targeting-the-urokinase-type-plasminogen-activator-receptor-upar
#16
Mathias Dyrberg Loft, Yao Sun, Changhao Liu, Camilla Christensen, Daijuan Huang, Andreas Kjaer, Zhen Cheng
The urokinase-type plasminogen activator receptor (uPAR) is overexpressed in several cancers including glioblastoma (GBM) and is an established biomarker for metastatic potential. The uPAR-targeting peptide AE105-NH2 (Ac-Asp-Cha-Phe-(D)Ser-(D)Arg-Tyr-Leu-Trp-Ser-CONH2) is a promising candidate for non-invasive positron emission tomography (PET) imaging of uPAR. Despite the optimal physical properties of (68)Ga for peptide-based PET imaging, low tumor uptakes have previously been reported using (68)Ga-labeled AE105-NH2-based tracers...
March 18, 2017: Amino Acids
https://www.readbyqxmd.com/read/28304288/changes-in-synaptic-plasticity-and-glutamate-receptors-in-type-2-diabetic-kk-ay-mice
#17
Huajing Yin, Weiping Wang, Wenwen Yu, Jiang Li, Nan Feng, Ling Wang, Xiaoliang Wang
In the present study, the progressive alteration of cognition and the mechanisms of reduction in long-term potentiation (LTP) in spontaneous obese KK-Ay type 2 diabetic mice were investigated. In the study, 3-, 5-, and 7-month-old KK-Ay mice were used. The results indicated that KK-Ay mice showed cognitive deficits in the Morris water maze test beginning at the age of 3 months. LTP was significantly impaired in KK-Ay mice during whole study period (3 to 7 months). The above deficits were reversible at an early stage (3 to 5 months old) by diet intervention...
March 18, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28300624/neurotoxicity-of-the-steroidal-alkaloids-tomatine-and-tomatidine-is-rip1-kinase-and-caspase-independent-and-involves-the-eif2%C3%AE-branch-of-the-endoplasmic-reticulum
#18
Daniela Correia da Silva, Paula B Andrade, Patrícia Valentão, David M Pereira
Steroidal alkaloids are a class of natural products that occur in several species of the Solanaceae family. In the case of tomato plant (Lycopersicon esculentum Mill.), tomatine and its aglycone, tomatidine, are the most representative molecules. These steroidal alkaloids have already shown several potentially useful biological activities, from anticancer to anti-inflammatory or antibacterial. In this work, the toxicity of these molecules in neuronal cells, namely in the neuroblastoma cell line SH-SY5Y, was assessed, emphasis being given to the cellular mechanisms underlying the effects observed...
March 11, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28300587/enzyme-inhibition-kinetics-and-molecular-interactions-of-patatin-peptides-with-angiotensin-i-converting-enzyme-and-renin
#19
Yu Fu, Adeola M Alashi, Jette F Young, Margrethe Therkildsen, Rotimi E Aluko
In this work, the inhibitory effects of potato patatin-derived peptides Trp-Gly (WG) and Pro-Arg-Tyr (PRY) on angiotensin I-converting enzyme (ACE) and renin activities were investigated using kinetics, intrinsic fluorescence and molecular docking. The results indicated that PRY was a more potent ACE- and renin-inhibitory peptide than WG. Enzyme inhibition kinetics showed that WG and PRY inhibited ACE activity through mixed-type and competitive modes, respectively. The inhibitory mechanism of WG and PRY towards renin was determined to be mixed-type...
March 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28297654/role-of-pore-lining-residues-in-defining-the-rate-of-water-conduction-by-aquaporin-0
#20
Patrick O Saboe, Chiara Rapisarda, Shreyas Kaptan, Yu-Shan Hsiao, Samantha R Summers, Rita De Zorzi, Danijela Dukovski, Jiaheng Yu, Bert L de Groot, Manish Kumar, Thomas Walz
Compared to other aquaporins (AQPs), lens-specific AQP0 is a poor water channel, and its permeability was reported to be pH-dependent. To date, most water conduction studies on AQP0 were performed on protein expressed in Xenopus oocytes, and the results may therefore also reflect effects introduced by the oocytes themselves. Experiments with purified AQP0 reconstituted into liposomes are challenging because the water permeability of AQP0 is only slightly higher than that of pure lipid bilayers. By reconstituting high amounts of AQP0 and using high concentrations of cholesterol to reduce the permeability of the lipid bilayer, we improved the signal-to-noise ratio of water permeability measurements on AQP0 proteoliposomes...
March 14, 2017: Biophysical Journal
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