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Ultrafiltration

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https://www.readbyqxmd.com/read/28444982/ionic-liquid-based-ultrasound-assisted-extraction-of-isoflavones-from-belamcanda-chinensis-and-subsequent-screening-and-isolation-of-potential-%C3%AE-glucosidase-inhibitors-by-ultrafiltration-and-semi-preparative-high-performance-liquid-chromatography
#1
Senlin Li, Sainan Li, Yu Huang, Chunming Liu, Lina Chen, Yuchi Zhang
The separation of a compound of interest from its structurally similar homologues to produce high-purity natural products is a challenging problem. This work proposes a novel method for the separation of iristectorigenin A from its structurally similar homologues by ionic liquid-based ultrasound-assisted extraction and the subsequent screening and isolation of potential α-glucosidase inhibitors via ultrafiltration and semi-preparative high-performance liquid chromatography. Ionic-liquid-based ultrasound-assisted extraction was successfully applied to the extraction of tectorigenin, iristectorigenin A, irigenin, and irisflorentin from Belamcanda chinensis...
April 26, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28439687/colloid-characterization-and-in-situ-release-in-shallow-groundwater-under-different-hydrogeology-conditions
#2
Jingjing Zhou, Dan Liu, Wenjing Zhang, Xuequn Chen, Ying Huan, Xipeng Yu
Changes to groundwater hydrodynamics and chemistry can lead to colloid release that can have a major impact on the groundwater environment. To analyze the effects of colloid release caused by artificial groundwater recharge, field and laboratory tests on colloid characterization and colloid release were conducted. The field tests were carried out at an artificial recharge test site in Shandong Province. In the field investigation, one recharge water sample and five groundwater samples were collected and filtered through three levels of ultrafiltration membranes, with pore sizes of 0...
April 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28434743/the-effect-of-emulsifying-salts-on-the-turbidity-of-a-diluted-milk-system-with-varying-ph-and-protein-concentration
#3
M D Culler, Y Saricay, F M Harte
Solutions of 10 commonly used emulsifying salts (ES) listed in the Code of Federal Regulations (21CFR133.179) for pasteurized process cheese were tested for their effect on the turbidity of a diluted milk system at different pH and protein concentrations to characterize the conditions that affect micellar structure. Emulsifying salt solutions were made by mixing the ES in a 1-in-20 dilution of water in skim milk ultrafiltrate (3 kDa molecular weight cut-off) to obtain ES concentrations from 0 to 248 mM. Skim milk was added to solutions containing nanopure water, skim milk ultrafiltrate, and a specific ES ranging in concentration from 0 to 248 mM and pH 5, 5...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434245/simultaneous-quantitation-of-the-bace1-inhibitor-azd3293-and-its-metabolite-az13569724-in-human-matrices-by-lc-ms-ms
#4
Yan Li, Paul H Severin, Mark R Hoffmann, Dennis L Miller, Scott A Monk, Alan R Kugler
AIM: AZD3293 is a novel BACE1 inhibitor in Phase III development for Alzheimer's disease. Sensitive and robust bioanalytical methods were required to quantitate AZD3293 and its metabolite AZ13569724 in human biological matrices. METHODOLOGY/RESULTS: Human plasma was prepared by protein precipitation. Linearity for both analytes was in the range of 0.5-500 ng/ml with up to 100-fold dilution. Plasma ultrafiltrate samples were prepared using Centrifree(®) ultrafiltration device...
April 24, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28432886/evaluation-of-biochar-ultrafiltration-membrane-processes-for-humic-acid-removal-under-various-hydrodynamic-ph-ionic-strength-and-pressure-conditions
#5
Vaibhavi Shankar, Jiyong Heo, Yasir A J Al-Hamadani, Chang Min Park, Kyoung Hoon Chu, Yeomin Yoon
The performance of an ultrafiltration (UF)-biochar process was evaluated in comparison with a UF membrane process for the removal of humic acid (HA). Bench-scale UF experiments were conducted to study the rejection and flux trends under various hydrodynamic, pH, ionic strength, and pressure conditions. The resistance-in-series model was used to evaluate the processes and it showed that unlike stirred conditions, where low fouling resistance was observed (28.7 × 10(12) m(-1) to 32.5 × 10(12) m(-1)), higher values and comparable trends were obtained for UF-biochar and UF alone for unstirred conditions (28...
April 19, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28431257/integrated-antimicrobial-and-antifouling-ultrafiltration-membrane-by-surface-grafting-peo-and-n-chloramine-functional-groups
#6
Shuhua Hou, Jialin Xing, Xue Dong, Jifu Zheng, Shenghai Li
Ultrafiltration membranes with integrated antimicrobial and antifouling properties were fabricated using an engineering thermoplastic (carboxylated cardopoly(aryl ether ketone, PEK-COOH). Different molecular weights of PEO (Mw: 120, 350, 550) were grafted to the PEK-COOH membrane surface via EDC/NHS methodology. N-chloramine modified membranes then were prepared by simple exposure to dilute sodium hypochlorite solution. The surface grafting processes were all performed in water (i.e. without organic solvent)...
April 11, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28427667/membrane-cleaning-with-ultrasonically-driven-bubbles
#7
Fabian Reuter, Sonja Lauterborn, Robert Mettin, Werner Lauterborn
A laboratory filtration plant for drinking water treatment is constructed to study the conditions for purely mechanical in situ cleaning of fouled polymeric membranes by the application of ultrasound. The filtration is done by suction of water with defined constant contamination through a membrane module, a stack of five pairs of flat-sheet ultrafiltration membranes. The short cleaning cycle to remove the cake layer from the membranes includes backwashing, the application of ultrasound and air flushing. A special geometry for sound irradiation of the membranes parallel to their surfaces is chosen...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28426078/an-intravascular-bioartificial-pancreas-device-ibap-with-silicon-nanopore-membranes-snm-for-islet-encapsulation-under-convective-mass-transport
#8
Shang Song, Charles Blaha, Willieford Moses, Jaehyun Park, Nathan Wright, Joey Groszek, William Fissell, Shant Vartanian, Andrew M Posselt, Shuvo Roy
Diffusion-based bioartificial pancreas (BAP) devices are limited by poor islet viability and functionality due to inadequate mass transfer resulting in islet hypoxia and delayed glucose-insulin kinetics. While intravascular ultrafiltration-based BAP devices possess enhanced glucose-insulin kinetics, the polymer membranes used in these devices provide inadequate ultrafiltrate flow rates and result in excessive thrombosis. Here, we report the silicon nanopore membrane (SNM), which exhibits a greater hydraulic permeability and a superior pore size selectivity compared to polymer membranes for use in BAP applications...
April 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28424739/rapid-screening-of-potential-phosphodiesterase-inhibitors-from-the-roots-of-ilex-pubescens-hook-et-arn-using-a-combination-of-ultrafiltration-and-lc-ms
#9
Zichen Liu, Zongtao Lin, Shizhong Chen, Lingjun Wang, Shaoxiang Xian
The cyclic nucleotide phosphodiesterase (PDE) plays an important role in regulating the levels of second messenger molecules cAMP and cGMP. Various PDE inhibitors have been successfully developed into drugs for targeted diseases. In addition, PDE inhibitors can also be found in different foods and natural medicines. In this study, ultrafiltration liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry (ultrafiltration LC-DAD-ESI-IT-TOF-MS) was applied to screen PDE inhibitors from the roots of Ilex pubescens Hook...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28422324/optical-resolution-and-mechanism-using-enantioselective-cellulose-sodium-alginate-and-hydroxypropyl-%C3%AE-cyclodextrin-membranes
#10
Li-Ming Yuan, Wei Ma, Mei Xu, Hui-Lin Zhao, Yuan-Yuan Li, Rui-Lin Wang, Ai-Hong Duan, Ping Ai, Xue-Xian Chen
Chiral solid membranes of cellulose, sodium alginate, and hydroxypropyl-β-cyclodextrin were prepared for chiral dialysis separations. After optimizing the membrane material concentrations, the membrane preparation conditions and the feed concentrations, enantiomeric excesses of 89.1%, 42.6%, and 59.1% were obtained for mandelic acid on the cellulose membrane, p-hydroxy phenylglycine on the sodium alginate membrane, and p-hydroxy phenylglycine on the hydroxypropyl-β-cyclodextrin membrane, respectively. To study the optical resolution mechanism, chiral discrimination by membrane adsorption, solid phase extraction, membrane chromatography, high-pressure liquid chromatography ultrafiltration were performed...
April 19, 2017: Chirality
https://www.readbyqxmd.com/read/28420862/clinical-characteristics-treatment-and-prognosis-of-patients-with-acute-severe-heart-failure-of-ischemic-and-non-ischemic-etiology-analysis-from-the-commit-ahf-registry
#11
Mateusz Ostręga, Marek J Gierlotka, Grzegorz Słonka, Paweł Nadziakiewicz, Mariusz Gąsior
INTRODUCTION    There are limited data on the impact of ischemic etiology on clinical status and long-term prognosis among patients with acute severe heart failure (ASHF) not associated with acute coronary syndrome (ACS).  OBJECTIVES    To assess clinical characteristics, treatment and the 12-month mortality of patients with no-ACS ASHF according to etiology.  PATIENTS AND METHODS    The data from 112 patients with ASHF that was not associated with ACS were analyzed: 61 with ischemic (IASHF) and 51 with non-ischemic (NIASHF) etiology...
April 18, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/28419668/prevalence-and-associated-variables-of-postdialysis-fatigue-results-of-a-prospective-multicenter-study
#12
Maurizio Bossola, Emanuele Marzetti, Enrico Di Stasio, Tania Monteburini, Stefano Cenerelli, Katia Mazzoli, Emanuele Parodi, Vittorio Sirolli, Stefano Santarelli, Fabio Ippoliti, Pier Eugenio Nebiolo, Mario Bonomini, Roberta Melatti, Carlo Vulpio
AIMS: Little is known about postdialysis fatigue (PDF), a debilitating symptom of hemodialysis patients. METHODS: In 5 hemodialysis units of northern-centre Italy, patients were regarded to suffer from PDF if they spontaneously offered this complaint when asked the open-ended question: Do you feel better or worse after dialysis? If worse, please specify in which way. A complaint of fatigue would be probed further with questions directed at its duration, frequency and intensity, allowing creation of a fatigue index of severity (one third of the sum of these three parameters, each rated from 1 to 5)...
April 17, 2017: Nephrology
https://www.readbyqxmd.com/read/28416553/pharmacokinetics-and-dialytic-clearance-of-ceftazidime-avibactam-in-a-critically-ill-patient-on-continuous-venovenous-hemofiltration
#13
Eric Wenzler, Kristen L Bunnell, Susan C Bleasdale, Scott Benken, Larry H Danziger, Keith A Rodvold
Ceftazidime-avibactam 1.25 g every 8 hours was used to treat multi-drug resistant Pseudomonas aeruginosa bacteremia in a critically ill patient on continuous venovenous hemofiltration (CVVH). Pre-filter plasma drug concentrations of ceftazidime and avibactam were measured at 0, 1, 2, 4, 6, and 8 hours along with post-filter and ultrafiltrate concentrations at hours 2 and 6. Plasma pharmacokinetic parameters of ceftazidime and avibactam, respectively, were Cmax 61.10 and 14.54 mg/L, Cmin 31.96 and 8.45 mg/L, t1/2 6...
April 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28415129/p1294evaluation-of-right-atrium-mechanics-and-relation-with-loading-conditions-by-speckle-tracking-echocardiography
#14
S Unlu
Purpose: The aim of this study is to evaluate the right atrial (RA) mechanics and change in echocardiographic parameters used for assessment of RA by examining the end stage kidney patients before and after the hemodialysis (HD). Methods: Patients between 18 and 85 years of age, receiving HD for at least 6 months were included. The echocardiographic images were obtained before and after hemodialysis therapy. Two-dimensional speckle tracking strain analysis was performed for right atrium in 62 patients...
December 1, 2016: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28413443/molecular-and-biochemical-characterization-of-recombinant-cel12b-cel8c-and-peh28-overexpressed-in-escherichia-coli-and-their-potential-in-biofuel-production
#15
Eman Ibrahim, Kim D Jones, Keith E Taylor, Ebtesam N Hosseney, Patrick L Mills, Jean M Escudero
BACKGROUND: The high crystallinity of cellulosic biomass myofibrils as well as the complexity of their intermolecular structure is a significant impediment for biofuel production. Cloning of celB-, celC-encoded cellulases (cel12B and cel8C) and peh-encoded polygalacturonase (peh28) from Pectobacterium carotovorum subsp. carotovorum (Pcc) was carried out in our previous study using Escherichia coli as a host vector. The current study partially characterizes the enzymes' molecular structures as well as their catalytic performance on different substrates which can be used to improve their potential for lignocellulosic biomass conversion...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28413417/the-dialysis-sodium-gradient-a-modifiable-risk-factor-for-fluid-overload
#16
Emilie Trinh, Catherine Weber
BACKGROUND: Fluid overload in patients on conventional hemodialysis is a frequent complication, associated with increased cardiovascular morbidity and mortality. The dialysate sodium prescription is a potential modifiable risk factor. Our primary objective was to describe associations between dialysate-to-serum sodium gradient and parameters of fluid status. A secondary objective was to evaluate the 6-month risk of hospitalization and mortality in relation to sodium gradient. METHODS: We performed a cross-sectional study of 110 prevalent conventional hemodialysis patients at a single center...
January 2017: Nephron Extra
https://www.readbyqxmd.com/read/28411466/capillary-isoelectric-focusing-with-whole-column-imaging-detection-icief-a-new-approach-to-the-characterization-and-quantification-of-salivary-%C3%AE-amylase
#17
Atefeh S Zarabadi, Tiemin Huang, John G Mielke
Saliva is an easily collected biological fluid with potentially important diagnostic value. While gel electrophoresis is generally used for salivary analysis, we employed the capillary isoelectric focusing technique to allow for a rapid, automated mode of electrophoresis. Capillary isoelectric focusing coupled with UV whole column imaging detection (iCIEF) was used to develop a robust protocol to characterize salivary α-amylase collected from various glands. Notably, three sample preparation methods were examined: ultrafiltration, gel-filtration, and starch affinity interaction with salivary amylase...
March 30, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28409034/increased-intracranial-pressure-during-hemodialysis-in-a-patient-with-anoxic-brain-injury
#18
Anton Lund, Mette B Damholt, Ditte G Strange, Jesper Kelsen, Hasse Møller-Sørensen, Kirsten Møller
Dialysis disequilibrium syndrome (DDS) is a serious neurological complication of hemodialysis, and patients with acute brain injury are at increased risk. We report a case of DDS leading to intracranial hypertension in a patient with anoxic brain injury and discuss the subsequent dialysis strategy. A 13-year-old girl was admitted after prolonged resuscitation from cardiac arrest. Computed tomography (CT) revealed an inferior vena cava aneurysm and multiple pulmonary emboli as the likely cause. An intracranial pressure (ICP) monitor was inserted, and, on day 3, continuous renal replacement therapy (CRRT) was initiated due to acute kidney injury, during which the patient developed severe intracranial hypertension...
2017: Case Reports in Critical Care
https://www.readbyqxmd.com/read/28407953/antioxidant-potential-of-edible-mushroom-agaricus-bisporus-protein-hydrolysates-and-their-ultrafiltration-fractions
#19
Benard Muinde Kimatu, Liyan Zhao, Yuan Biao, Gaoxing Ma, Wenjian Yang, Fei Pei, Qiuhui Hu
Mushroom protein isolate (MPI) from Agaricus bisporus was hydrolyzed using Alcalase, Pancreatin, Flavourzyme, Alcalase-Pancreatin and Alcalase-Flavourzyme. The obtained hydrolysates (MPHs) were ultrafiltered to generate peptide fractions (UFs) of molecular sizes (<1, 1-3, 3-5 and 5-10kDa). The electrophoretic profile results indicated that the enzymatic systems were efficient in hydrolyzing the MPI into low molecular weight peptides. Hydrolysate yields of >57% and protein recoveries of >43% were obtained...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407942/effect-of-ultrasound-pretreatment-and-maillard-reaction-on-structure-and-antioxidant-properties-of-ultrafiltrated-smooth-hound-viscera-proteins-sucrose-conjugates
#20
Ola Abdelhedi, Leticia Mora, Ines Jemil, Mourad Jridi, Fidel Toldrá, Moncef Nasri, Rim Nasri
The effect of ultrasound (US) pre-treatment on the evolution of Maillard reaction (MR), induced between low molecular weight (LMW) peptides and sucrose, was studied. LMW peptides (<1kDa) were obtained by the ultrafiltration of smooth hound viscera protein hydrolysates, produced by Neutrase, Esperase and Purafect. MR was induced by heating the LMW peptides in the presence of sucrose for 2h at 90°C, without or with US pre-treatment. During the reaction, a marked decrease in pH values, coupled to the increase in colour of the Maillard reaction products (MRPs), were recorded...
September 1, 2017: Food Chemistry
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