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Ultrafiltration

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https://www.readbyqxmd.com/read/28534129/insomnia-muscular-cramps-and-pruritus-have-low-intensity-in-hemodialysis-patients-with-good-dialysis-efficiency-low-inflammation-and-arteriovenous-fistula
#1
Olga Hilda Orasan, Aniela Pop Saplontai, Angela Cozma, Simona Racasan, Ina Maria Kacso, Crina Claudia Rusu, Diana Moldovan, Dacian Tirinescu, Alina Potra, Ioan Mihai Patiu, Remus Aurel Orasan
INTRODUCTION: Insomnia, muscular cramps, pruritus and postdialysis recovery time (RT) are quality-of-life parameters that affect hemodialysis (HD) patients physically and mentally. METHODS: We included 171 end-stage renal disease patients: 115 on high-flux HD and 56 on online hemodiafiltration (HDF). Patients were asked "How long does it take you to recover from a dialysis session?" and they evaluated intensity (absent, mild, medium and severe) of insomnia, muscular cramps and pruritus in the past 4 weeks...
May 22, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28534128/cathepsin-l-activity-correlates-with-proteinuria-in-chronic-kidney-disease-in-humans
#2
Yu Cao, Xing Liu, Ying Li, Yao Lu, Hua Zhong, Weihong Jiang, Alex F Chen, Timothy R Billiar, Hong Yuan, Jingjing Cai
BACKGROUND: The presence and severity of proteinuria is considered an important prognostic marker in patients with chronic kidney disease (CKD) and is associated with mortality and morbidity. Cathepsin L is highly expressed in the foot processes of podocytes in the kidney, which serves as an ultrafiltration barrier. Cathepsin L is also up-regulated in the setting of inflammation as a feature of CKD. Therefore, we postulated that proteinuria severity in CKD patients might correlate with increased serum levels of cathepsin L...
May 22, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28532077/preparation-of-robust-braid-reinforced-poly-vinyl-chloride-ultrafiltration-hollow-fiber-membrane-with-antifouling-surface-and-application-to-filtration-of-activated-sludge-solution
#3
Zhuang Zhou, Saeid Rajabzadeh, Lifeng Fang, Taro Miyoshi, Yuriko Kakihana, Hideto Matsuyama
Braid-reinforced hollow fiber membranes with high mechanical properties and considerable antifouling surface were prepared by blending poly(vinyl chloride) (PVC) with poly(vinyl chloride-co-poly(ethylene glycol) methyl ether methacrylate) (poly(VC-co-PEGMA)) copolymer via non-solvent induced phase separation (NIPS). The tensile strength of the braid-reinforced PVC hollow fiber membranes were significantly larger than those of previously reported various types of PVC hollow fiber membranes. The high interfacial bonding strength indicated the good compatibility between the coating materials and the surface of polyethylene terephthalate (PET)-braid...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28530588/capillary-electrophoresis-coupled-to-mass-spectrometry-employing-hexafluoro-2-propanol-for-the-determination-of-nucleosides-and-nucleotide-mono-di-and-tri-phosphates-in-baby-foods
#4
María Mateos-Vivas, Javier Domínguez-Álvarez, Encarnación Rodríguez-Gonzalo, Rita Carabias-Martínez
The present work describes a method for the simultaneous determination of unmodified nucleosides and nucleotide mono-, di- and tri-phosphates by capillary electrophoresis coupled to mass spectrometry (CE-MS). The use of hexafluoro-2-propanol (HFIP) in the separation medium, and as an additive to the sheath liquid of the electrospray interface (ESI), generated a highly efficient and sensitive method. Instrumental limits of detection in the range of 14-53ngmL(-1) for nucleosides and 7-23, 20-49 and 64-124ngmL(-1) for nucleotide mono-, di-, and tri-phosphates, respectively, were found...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28527978/enhanced-antimony-v-removal-using-synergistic-effects-of-fe-hydrolytic-flocs-and-ultrafiltration-membrane-with-sludge-discharge-evaluation
#5
Baiwen Ma, Xing Wang, Ruiping Liu, Zenglu Qi, William A Jefferson, Huachun Lan, Huijuan Liu, Jiuhui Qu
The integration of adsorbents with ultrafiltration (UF) membranes is a promising method for alleviating membrane fouling and reducing land use. However, a number of problems have become apparent concerning the granular adsorbents used currently, such as high running cost, high chance of causing membrane surface damage, low in situ chemical cleaning efficiency, etc. Herein, to overcome these disadvantages, loose in situ hydrolyzed flocs were directly injected into the membrane tank, providing strong adsorption ability at low cost...
May 13, 2017: Water Research
https://www.readbyqxmd.com/read/28527829/graphene-oxide-nanosheets-and-d-%C3%AE-tocopheryl-polyethylene-glycol-1000-succinate-tpgs-doping-improves-biocompatibility-and-ultrafiltration-in-polyethersulfone-hollow-fiber-membranes
#6
Akshay Modi, Surendra Kumar Verma, Jayesh Bellare
Novel graphene oxide (G)-and d-α-Tocopheryl polyethylene glycol 1000 succinate (T)-doped polyethersulfone (P) hollow fiber membranes (GTP HFMs) were efficiently prepared. GTP HFMs were found to be a desirable biocompatible substrate for attachment and proliferation of human embryonic kidney-293 (HEK-293) cells. Significantly high porosity (94.58±1.1%), low contact angle (61.1±2.5°), low hemolysis (0.58% in batch mode and 0.64% in continuous mode), low terminal complement complex activation (SC5b-9 marker level ∼6...
May 12, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28527288/-partial-purification-of-peptides-present-in-the-tityus-macrochirus-buthidae-scorpion-venom-and-preliminary-assessment-of-their-cytotoxicity
#7
Clara Andrea Rincón-Cortés, Edgar Antonio Reyes-Montaño, Nohora Angélica Vega-Castro
INTRODUCTION: Scorpion venom contains peptides with neurotoxic action primarily active on ion channels in the nervous system of insects and mammals. They are also characterized as cytolytic and anticancer, biological characteristics that have not yet been reported for the Tityus macrochirus venom. OBJECTIVE: To assess if the total T. macrochirus venom and the fraction of partially purified peptides decrease the viability of various tumor-derived cell lines. MATERIALS AND METHODS: The scorpion venom was collected by electrical stimulation and, subsequently, subjected to chromatography, electrophoresis, and ultrafiltration with Amicon Ultra 0...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28525882/interfacially-crosslinked-composite-porous-membranes-for-ultrafast-removal-of-anionic-dyes-from-water-through-permeating-adsorption
#8
Cuijing Liu, Liang Cheng, Yifan Zhao, Liping Zhu
The dye wastewater is one of the most difficult industrial wastewaters to treat. It keeps a big challenge to realize fast removal of dyes from water by membrane filtration due to the trade-off between separation selectivity and permeation flux for ultrafiltration or nanofiltration (NF) process. Here we report novel composite porous membranes which can remove anionic dyes from water by ultrafast permeating adsorption. A crosslinked polyethyleneimine (PEI) polymer with strong adsorption ability was incorporated onto a nylon microfiltration membrane by the interfacial amidation reaction between PEI and trimesoyl chloride...
April 12, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28525669/relationship-between-bioimpedance-determined-body-composition-and-peritoneal-transport-in-peritoneal-dialysis
#9
Bernardo M da Costa, Gloria Del Peso, Maria Auxiliadora Bajo, Gilda Carreño, Marta Ferreira, Carina Ferreira, Rafael Selgas
PURPOSE: In peritoneal dialysis (PD) patients, body fluid homeostasis is dependent on peritoneal elimination of water and solutes. Patients with less favorable peritoneal transport parameters should be more overhydrated. Despite this, the association between faster transport and overhydration (OH) is weak, and the factors that influence hydration status are still poorly characterized. Modified peritoneal equilibration tests (PET) offer us new parameters that might correlate better with hydration status, like free water transport (FWT)...
May 18, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28524241/fabrication-techniques-enabling-ultrathin-nanostructured-membranes-for-separations
#10
REVIEW
Marcela Mireles Ramirez, Thomas R Gaborski
The fabrication of nanostructured materials is an area of continuous improvement and innovative techniques that fulfill the demand of many fields of research and development. The continuously decreasing size of the smallest patternable feature has expanded the catalog of methods enabling the fabrication of nanostructured materials. Several of these nanofabrication techniques have sprouted from applications requiring nanoporous membranes such as molecular separations, cell culture, and plasmonics. This review summarizes methods that successfully produce through-pores in ultrathin films exhibiting an approximate pore size to thickness ratio of one, which has been shown to be beneficial due to high permeability and improved separation potential...
May 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28501217/edta-functionalized-magnetic-nanoparticles-a-suitable-platform-for-the-analysis-of-low-abundance-urinary-proteins
#11
Paulo Bastos, Fábio Trindade, Rita Ferreira, Adelino Leite-Moreira, Inês Falcão-Pires, Bruno Manadas, Ana L Daniel-da-Silva, Rui Vitorino
Urine is a highly attractive source of biological information and disease biomarkers, whose proteome characterization is ongoing. To that end, depletion/enrichment strategies for protein analysis can be of great convenience. We have thus developed a method based on the use of EDTA-functionalized magnetic nanoparticles (NPs@EDTA), to fractionate urine samples before liquid chromatography-mass spectrometry analysis and compared the identified proteins with those obtained from ultrafiltrated/unfractionated (UF) urine samples...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28501082/a-high-content-screening-technology-for-quantitatively-studying-podocyte-dynamics
#12
REVIEW
Jochen Reiser, Ha Won Lee, Vineet Gupta, Mehmet M Altintas
Podocytes form the visceral layer of a kidney glomerulus and express a characteristic octopus-like cellular architecture specialized for the ultrafiltration of blood. The cytoskeletal dynamics and structural elasticity of podocytes rely on the self-organization of highly interconnected actin bundles, and the maintenance of these features is important for the intact glomerular filtration. Development of more differentiated podocytes in culture has dramatically increased our understanding of the molecular mechanisms regulating podocyte actin dynamics...
May 2017: Advances in Chronic Kidney Disease
https://www.readbyqxmd.com/read/28499668/a-multicenter-feasibility-study-on-ultrafiltration-via-a-single-peripheral-venous-access-in-acute-heart-failure-with-overt-fluid-overload
#13
Marco Morpurgo, Mario Pasqualini, Maria Cristiana Brunazzi, Gabriele Vianello, Roberto Valle, Loris Roncon, Fulvio Fiorini, Nadia Aspromonte, Mario Barbiero, Marco Goldoni, Giancarlo Marenzi
OBJECTIVES: The need for a central venous catheter has limited the widespread use of ultrafiltration in daily clinical practice for the treatment of acute heart failure (AHF) with overt fluid overload. We evaluated the feasibility of a new ultrafiltration device, the CHIARA (Congestive Heart Impairment Advanced Removal Approach) system, that utilizes a single-lumen cannula (17G, multi-hole) inserted in a peripheral vein of the arm. METHODS: In this multicenter, prospective, feasibility study, consecutive ultrafiltration treatments (lasting ≥6 hours and with an ultrafiltration rate ≥100ml/h) with the CHIARA device and a single peripheral venous approach were performed at 6 Italian hospitals...
May 2, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28499179/fluorescent-natural-organic-matter-responsible-for-ultrafiltration-membrane-fouling-fate-contributions-and-fouling-mechanisms
#14
Hui Wang, An Ding, Zhendong Gan, Fangshu Qu, Xiaoxiang Cheng, Langming Bai, Shaodong Guo, Guibai Li, Heng Liang
Membrane fouling has been a main obstacle to the success of ultrafiltration (UF) technology. Recently, fluorescent natural organic matter (FNOM), including humic-like substances (HS) and protein-like substances, has been recognized as substances responsible for membrane fouling. In this study, the matrix of FNOM in natural river water was substantially modified by combined coagulation and powdered activated carbon adsorption to enhance the diversity of the FNOM matrix. Fluorescence excitation emission matrix spectroscopy was employed to characterize FNOM components during the UF process...
May 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28497137/identification-of-two-novel-antioxidant-peptides-from-edible-bird-s-nest-aerodramus-fuciphagus-protein-hydrolysates
#15
Masomeh Ghassem, Keizo Arihara, Salimeh Mohammadi, Norrakiah Abdullah Sani, Abdul Salam Babji
Edible bird's nest (EBN) is widely consumed as a delicacy and traditional medicine amongst the Chinese. In the present study, for the first time, the antioxidant properties of an EBN pepsin-trypsin hydrolysate of the swiftlet species Aerodramus fuciphagus and its ultrafiltration fractions were investigated. Thirteen peptides with molecular weights between 514.29 and 954.52 Da were identified in the EBN fraction with the use of mass spectrometry. Two novel pentapeptides Pro-Phe-His-Pro-Tyr and Leu-Leu-Gly-Asp-Pro, corresponding to f134-138 and f164-168 of cytochrome b of A...
May 24, 2017: Food & Function
https://www.readbyqxmd.com/read/28496409/rapid-screening-for-%C3%AE-glucosidase-inhibitors-from-gymnema-sylvestre-by-affinity-ultrafiltration-hplc-ms
#16
Guilin Chen, Mingquan Guo
Gymnema sylvestre R. Br. (Asclepiadaceae) has been known to posses potential anti-diabetic activity, and the gymnemic acids were reported as the main bioactive components in this plant species. However, the specific components responsible for the hypoglycemic effect still remain unknown. In the present study, the in vitro study revealed that the extract of G. sylvestre exhibited significant inhibitory activity against α-glucosidase with IC50 at 68.70 ± 1.22 μg/mL compared to acarbose (positive control) at 59...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28494980/extracorporeal-ultrafiltration-for-fluid%C3%A2-overload-in-heart-failure-current-status-and-prospects-for-further-research
#17
REVIEW
Maria Rosa Costanzo, Claudio Ronco, William T Abraham, Piergiuseppe Agostoni, Jonathan Barasch, Gregg C Fonarow, Stephen S Gottlieb, Brian E Jaski, Amir Kazory, Allison P Levin, Howard R Levin, Giancarlo Marenzi, Wilfried Mullens, Dan Negoianu, Margaret M Redfield, W H Wilson Tang, Jeffrey M Testani, Adriaan A Voors
More than 1 million heart failure hospitalizations occur annually, and congestion is the predominant cause. Rehospitalizations for recurrent congestion portend poor outcomes independently of age and renal function. Persistent congestion trumps serum creatinine increases in predicting adverse heart failure outcomes. No decongestive pharmacological therapy has reduced these harmful consequences. Simplified ultrafiltration devices permit fluid removal in lower-acuity hospital settings, but with conflicting results regarding safety and efficacy...
May 16, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28494541/-new-therapeutic-paradigm-and-concepts-for-patients-with-cirrhotic-refractory-ascites
#18
S Z Wang, H G Ding
The activation of renin-angiotensin-aldosterone-vasopressin system is a key factor in the formation of ascites due to splanchnic vasodilation in cirrhosis. In theory, aldosterone antagonists, contraction of blood vessels, vasopressin V2 receptor, and angiotensin receptor antagonists are important targets for the prevention and treatment of cirrhotic ascites. The 15%-20% of patients with cirrhotic ascites that show no response to at least one week's treatment with potent diuretics (spironolactone 160 mg/d combined with furosemide 80 mg/d) are considered to have refractory ascites...
April 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28493131/filter-aided-sample-preparation-fasp-for-improved-proteome-analysis-of-recombinant-chinese-hamster-ovary-cells
#19
Orla Coleman, Michael Henry, Martin Clynes, Paula Meleady
Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell line for biopharmaceutical production because of their ability to correctly fold and posttranslationally modify recombinant proteins that are compatible with human use. Proteomics, along with other 'omic platforms, are being used to understand the biology of CHO cells with the ultimate aim of enhancing CHO cell factories for more efficient production of biopharmaceuticals. In this chapter, we will describe an efficient protocol called Filter Aided Sample Preparation (FASP) for the extraction of proteins from CHO cells for proteomic studies...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28490530/il-6-promotes-epithelial-to-mesenchymal-transition-of-human-peritoneal-mesothelial-cells-possibly-through-jak2-stat3-signaling-pathway
#20
Jing Xiao, Yanan Gong, Ying Chen, Dahai Yu, Xiaoyang Wang, Xiaoxue Zhang, Yanna Dou, Dong Liu, Genyang Cheng, Shan Lu, Wenming Yuan, Yansheng Li, Zhanzheng Zhao
Long-term peritoneal dialysis (PD) therapy results in functional and structural alteration of the peritoneal membrane, including epithelial-to-mesenchymal transition (EMT). Interleukin 6 (IL-6) is a local pleiotropic cytokine, hypothesized to play an important role in EMT. This study was designed to investigate the role of IL-6 in EMT and peritoneal membrane dysfunction in long-term PD patients by assessing the level of IL-6 in dialysate and exploring the relationship between IL-6, the related signaling pathway JAK2/STAT3, and EMT, using in vitro cellular and molecular techniques...
May 10, 2017: American Journal of Physiology. Renal Physiology
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