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https://www.readbyqxmd.com/read/28628119/differential-expression-of-mir16-in-glioblastoma-and-glioblastoma-stem-cells-their-correlation-with-proliferation-differentiation-metastasis-and-prognosis
#1
R Tian, J Wang, H Yan, J Wu, Q Xu, X Zhan, Z Gui, M Ding, J He
The function of miR16 in multiforme glioblastoma multiforme (GBM) and its stem cells (GSCs) remains elusive. To this end, we investigated the patterns of miR16 expression in these cells and their correlation with malignant behaviors and clinical outcomes. The levels of miR16 and its targeted genes in tumor tissue of GBM and GBM SGH44, U87, U251 cells as well as their stem cell counterparts were measured by qRT-PCR or western blot or immunohistochemistry. Luciferase reporter assay was used to confirm the binding of miR16 to 3'-UTR of its target genes...
June 19, 2017: Oncogene
https://www.readbyqxmd.com/read/28627702/lentivirus%C3%A2-mediated-knockdown-of-chondroitin-polymerizing-factor-inhibits-glioma-cell-growth-in%C3%A2-vitro
#2
Yang-Hua Fan, Bing Xiao, Shi-Gang Lv, Min-Hua Ye, Xin-Gen Zhu, Miao-Jing Wu
Glioma is the most common primary tumor in the central nervous system, characterized by rapid progression, aggressive behavior, frequent recurrence and poor prognosis. In the present study we demonstrated that chondroitin polymerizing factor (CHPF) is highly expressed in human glioma tissues and 4 glioma cell lines. To explore the role of CHPF in glioma, a lentiviral vector expressing CHPF shRNA was constructed and transfected into the glioma U251 cells, which stably downregulated the expression levels of the CHPF gene in U251 cells in vitro...
June 19, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28624794/knockdown-of-hdac1-expression-suppresses-invasion-and-induces-apoptosis-in-glioma-cells
#3
Xiao-Qiang Wang, Hong-Min Bai, Shi-Ting Li, Hui Sun, Ling-Zhao Min, Bang-Bao Tao, Jun Zhong, Bin Li
Glioma is the most common malignant tumor of the central nervous system, with a low survival rate of five years worldwide. Although high expression and prognostic value of histone deacetylase 1 (HDAC1) have been recently reported in various types of human tumors, the molecular mechanism underlying the biological function of HDAC1 in glioma is still unclear. We found that HDAC1 was elevated in glioma tissues and cell lines. HDAC1 expression was closely related with pathological grade and overall survival of patients with gliomas...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28624783/malignant-pericytes-expressing-gt198-give-rise-to-tumor-cells-through-angiogenesis
#4
Liyong Zhang, Yan Wang, Mohammad H Rashid, Min Liu, Kartik Angara, Nahid F Mivechi, Nita J Maihle, Ali S Arbab, Lan Ko
Angiogenesis promotes tumor development. Understanding the crucial factors regulating tumor angiogenesis may reveal new therapeutic targets. Human GT198 (PSMC3IP or Hop2) is an oncoprotein encoded by a DNA repair gene that is overexpressed in tumor stromal vasculature to stimulate the expression of angiogenic factors. Here we show that pericytes expressing GT198 give rise to tumor cells through angiogenesis. GT198+ pericytes and perivascular cells are commonly present in the stromal compartment of various human solid tumors and rodent xenograft tumor models...
May 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28615958/the-critical-role-of-egf-%C3%AE-catenin-signaling-in-the-epithelial-mesenchymal-transition-in-human-glioblastoma
#5
Xingqiang Wang, Shanshi Wang, Xiaolong Li, Shigang Jin, Feng Xiong, Xin Wang
To date, β-catenin has been reported to be implicated in mediating the epithelial-mesenchymal transition (EMT) in a variety of human cancers, which can be triggered by EGF. However, the mechanisms underlying EGF-β-catenin pathway-induced EMT of glioblastoma multiforme (GBM) have not been reported previously. In the present study, immunohistochemistry, reverse transcription polymerase chain reaction, and Western blot were applied to investigate the effect of EGF-β-catenin pathway on EMT of GBM. Here, we identified that β-catenin mRNA and protein levels were up-regulated in GBM tissues and four kinds of glioblastoma cell lines, including T98G, A172, U87, and U251 cells, compared with normal brain tissue and astrocytes...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28615614/effects-of-connexin43-overexpression-on-u251-cell-growth-migration-and-apoptosis
#6
Jia Lu, Mingyue Yu, Zhen Lin, Su Lue, Hao Zhang, Hang Zhao, Yanyan Xu, Haiyan Liu
BACKGROUND Glioblastoma multiforme (GBM) is a highly aggressive malignant brain tumor with a high incidence in adults. Connexin43 (Cx43) has general roles in tumorigenesis and is expressed in U251 glioma cells. Accordingly, the effects of Cx43 on the growth, migration, and apoptosis and the underlying mechanisms mediating Cx43-dependent migration and apoptosis were examined in U251 cells. MATERIAL AND METHODS A Cx43-overexpressing U251 cell line was generated to analyze the effects of Cx43 overexpression on cell growth, wound healing, and apoptosis-related protein expression after treatment with temozolomide...
June 15, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28610554/slc7a11-over-expression-in-glioblastoma-is-associated-with-increased-cancer-stem-cell-like-properties
#7
Monika Dagmara Polewski, Rosyli Francis Reveron-Thornton, Gregory Allan Cherryholmes, Georgi K Marinov, Karen Saida Aboody
System xc- is a sodium independent electro-neutral transporter, composed of a catalytic subunit xCT (SLC7A11) which is involved in importing cystine. Certain cancers such as gliomas up-regulate the expression of system xc- which confers a survival advantage against the detrimental effects of reactive oxygen species (ROS) by increasing the generation of the antioxidant glutathione. However, ROS have also been shown to function as targeted, intracellular second messengers in an array of physiological processes such as proliferation...
June 13, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28602292/high-sensitive-bioorthogonal-sers-tag-for-live-cancer-cell-imaging-by-self-assembling-core-satellites-structure-gold-silver-nanocomposite
#8
Meng Chen, Ling Zhang, Mingxia Gao, Xiangmin Zhang
A novel, high-sensitivity, biocompatible SERS tag with core-shell structure based on gold nanoparticles containing alkynyl molecule core -silver nanoparticle satellites shell was fabricated for the first time to be used for live cancer cells Surface enhanced Raman scattering (SERS) imaging. (E)-2-((4-(phenylethynyl)benzylidene) amino) ethanethiol (PBAT) synthesized facilely in our lab is the Raman-silence region reporter which is advantage for bioorthogonal SERS cell imaging. In order to enhance the intensity of the Raman tags for live cancer cell imaging, a series of news measures have been adopted...
September 1, 2017: Talanta
https://www.readbyqxmd.com/read/28595907/mir-141-3p-functions-as-a-tumor-suppressor-modulating-activating-transcription-factor-5-in-glioma
#9
Mengyuan Wang, Ming Hu, Zhaohua Li, Dongmeng Qian, Bin Wang, David X Liu
Glioma is the most common malignant primary brain tumor which arises from the central nervous system. Our studies reported that an anti-apoptotic factor, activating transcription factor 5 (ATF5), is highly expressed in malignant glioma specimens and cell lines. Downregulation by dominant-negetive ATF5 could repress glioma cell proliferation and accelerate apoptosis. Here, we further investigate the upstream factor which regulates ATF5 expression. Bioinformatic analysis showed that ATF5 was a potential target of miR-141-3p...
June 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28583903/interleukin-13-conjugated-quantum-dots-for-identification-of-glioma-initiating-cells-and-their-extracellular-vesicles
#10
A B Madhankumar, Oliver Mrowczynski, Suhag Patel, Cody Weston, Brad Zacharia, Michael Glantz, Christopher Siedlecki, Lichong Xu, James R Connor
Cadmium selenide (CdSe) based quantum dots modified with polyethylene glycol and chemically linked to interleukin-13 (IL13) were prepared with the aim of identifying the high affinity receptor (IL13Rα2) which is expressed in glioma stem cells and exosomes secreted by these cancer stem cells. IL13 conjugated quantum dots (IL13QD) were thoroughly characterized for their physicochemical properties including particle size and surface morphology. Furthermore, the specific binding of the IL13QD to glioma cells and to glioma stem cells (GSC) was verified using a competitive binding study...
June 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28581641/clk1-regulated-aerobic-glycolysis-is-involved-in-gliomas-chemoresistance
#11
Li Zhang, Huicui Yang, Wenbin Zhang, Zhongqin Liang, Qiang Huang, Guoqiang Xu, Xuechu Zhen, Long Tai Zheng
Chemoresistance remains a major challenge for the treatment of glioma. In the present study, we investigated the role of Clk1, which encodes an enzyme that is necessary for ubiquinone biosynthesis in glioma chemoresistance in vitro. The results showed that Clk1 was highly expressed in GL261 mouse glioma cells which were most sensitive to BCNU while was expressed in BCNU resistant cells such as glioma cancer stem cells: T98G, U87MG and U251 glioma cells at low levels. Knockdown of Clk1 in GL261 glioma cells significantly reduced BCNU- or cisplatin- induced cell apoptosis whereas the proliferative activity and the expression of multi-drug resistance -related genes including MDR1, MGMT and GSTP1 were not changed...
June 5, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28569417/down-regulation-of-aqp4-expression-via-p38-mapk-signaling-in-temozolomide-induced-glioma-cells-growth-inhibition-and-invasion-impairment
#12
Chen Yuqin, Gao Fei, Jiang Rong, Liu Hui, Hou Jiaojiao, Yi Yaoxing, Kang Lili, Liu Xueyuan, Li Yuan, Yang Mei
Glioma is the most common and lethal central nervous system tumors. Temozolomide (TMZ) is an effective drug for malignant glioma, however, the intracellular and molecular mechanisms behind this anti-cancer effect have yet to be fully understood. The aim of the present study was to determine whether TMZ inhibits proliferation, invasion of glioma cells in vitro and whether these effects can be mediated through modulation of aquaporin 4 (AQP4) and phosphorylation of the MAPK pathway. The viability of U87 and U251 human glioma cells was evaluated using MTT assay...
June 1, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28560379/vpa-and-mel-induce-apoptosis-by-inhibiting-the-nrf2-are-signaling-pathway-in-tmz-resistant-u251-cells
#13
Hao Pan, Handong Wang, Yue Jia, Qiang Wang, Liwen Li, Qi Wu, Longbang Chen
Chemoresistance is the primary obstacle to effective treatment of glioblastoma, the most lethal brain tumor. Our previous study demonstrated that Nf-E2 related factor 2 (Nrf2), a traditional cytoprotective transcription factor, was overexpressed in gliomas and promoted malignancy. The present study aimed to investigate the expression levels of Nrf2‑antioxidant response element (ARE) signaling pathway genes in temozolomide (TMZ)‑resistant U251 human glioblastoma cells (U251‑TMZ). Additionally, the effect of valproic acid (VPA) and melatonin (MEL) on Nrf2 expression in U251‑TMZ cells and their association with chemoresistance was investigated...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28559969/escape-of-u251-glioma-cells-from-temozolomide-induced-senescence-was-modulated-by-cdk1-survivin-signaling
#14
Zhenhua Song, Yunyun Pan, Gengqiang Ling, Shiyong Wang, Min Huang, Xiaodan Jiang, Yiquan Ke
Temozolomide (TMZ) has been widely used in conjunction with radiotherapy for treating various types of cancers. However, tumor cells arrested in senescence due to TMZ administration can sometimes escape and become drug resistant. In the current study, the possible role of survivin in the senescence escape of TMZ-treated glioma cells was comprehensively studied. The levels of survivin and CDK1 expression in a human glioma cell line (U251) were monitored, and cell apoptosis, cell cycle distribution, anchorage-independent growth, and senescence were studied in U251 cells in different degrees of senescence...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28551849/long-non-coding-rna-malat1-promotes-proliferation-and-suppresses-apoptosis-of-glioma-cells-through-derepressing-rap1b-by-sponging-mir-101
#15
Zhenjiang Li, Chenyang Xu, Bingqian Ding, Ming Gao, Xinting Wei, Nan Ji
Long non-coding RNAs (lncRNAs) have been recently shown to be dysregulated and closely related to several cancers. Here, we aimed to elucidate the function and the possible molecular mechanisms of lncRNA Metastasis-associated lung Adenocarcinoma transcript-1 (MALAT1) in human glioma. Quantitative real-time PCR (qRT-PCR) was used to detect the expressions of MALAT1, miR-101 and Rap1B mRNA in U251 and U87 cells. The protein level of Rap1B was examined by western blot assays. Moreover, the proliferation and apoptosis of U251 and U87 cells were determined by CCK-8 assay and flow cytometry analysis, respectively...
May 27, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28548636/long-non-coding-rna-uca1-targets-mir-122-to-promote-proliferation-migration-and-invasion-of-glioma-cells
#16
Yang Sun, Jun-Gong Jin, Wei-Yang Mi, Hao Wu, Shi-Rong Zhang, Qiang Meng, Shi-Tao Zhang
Glioma is the most common and lethal intracranial malignant tumor. Long non-coding RNAs (lncRNAs) have been identifiedas pivotal regulators in the tumorigenesis of glioma. However, the role of lncRNA urothelial carcinoma-associated 1 (UCA1) in the gliomagenesis is still unknown. The purpose of this study is to investigate the underlying function of UCA1 on glioma genesis. Results demonstrated that UCA1 was up-regulated in glioma tissue and indicated the poor prognosis. UCA1 knockdown induced by si-UCA1 significantly suppressed the proliferative, migrative and invasive activity of glioma cell lines (U87 and U251)...
May 5, 2017: Oncology Research
https://www.readbyqxmd.com/read/28535003/mirna%C3%A2-1271-inhibits-cell-proliferation-in-neuroglioma-by-targeting-fibronectin-1
#17
Jian Gong, Zhao-Xia Wang, Zhen-Ying Liu
miR-1271 is a multifunctional post-translational modulator, which is involved in several diseases. However, the association between microRNA (miR)‑1271 and fibronectin 1 (FN1) remains to be fully elucidated in neuroglioma. In the present study, it was hypothesized that a post‑translational mechanism of miR‑1271 regulates the expression of FN1 in the progression of neuroglioma. The present study aimed to investigate the clinical significance and underlying molecular mechanisms of miRNA‑1271 in the development of glioma...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534933/cuedc2-suppresses-glioma-tumorigenicity-by-inhibiting-the-activation-of-stat3-and-nf-%C3%AE%C2%BAb-signaling-pathway
#18
Feng Li, Chuanxi Tang, Dan Jin, Li Guan, Yue Wu, Xinfeng Liu, Xiuxiang Wu, Qing Yun Wu, Dianshuai Gao
CUEDC2, a CUE domain containing 2 protein, plays critical roles in many biological processes, such as cell cycle, inflammation and tumorigenesis. However, whether CUEDC2 was involved in tumorigenesis of glioma and the possible mechanism remains to be elucidated. In the present study, our results implied that the expression of CUEDC2 was lower in the glioma tissue and glioma cell lines than that of normal tissue and asctrocyte cells. Downregulation of endogenous CUEDC2 in glioma U251 cell lines by RNAi promoted the tumor cells proliferation, migration, invasion and glioma neurosphere formation, while, overexpression of CUEDC2 showed the opposite effect...
May 17, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28530245/shifting-the-optimal-stiffness-for-cell-migration
#19
Benjamin L Bangasser, Ghaidan A Shamsan, Clarence E Chan, Kwaku N Opoku, Erkan Tüzel, Benjamin W Schlichtmann, Jesse A Kasim, Benjamin J Fuller, Brannon R McCullough, Steven S Rosenfeld, David J Odde
Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches: embryonic chick forebrain neurons (ECFNs; optimum ∼1 kPa) and U251 glioma cells (optimum ∼100 kPa)...
May 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28528183/effects-of-quercetin-on-proliferation-and-migration-of-human-glioblastoma-u251-cells
#20
Yue Liu, Zhen-Gang Tang, Yi Lin, Xin-Guo Qu, Wei Lv, Guo-Bin Wang, Cai-Li Li
Quercetin is a flavonoid that has been shown to have anti-oxidation, anti-inflammation, anti-allergic, anti-viral, and anti-cancer activities. Here, we examined the effects of quercetin on cell viability, cell cycle progression, and migration in U251 cells, a human glioblastoma cell line. We found that quercetin inhibited cell proliferation after treating cells for 24 (IC50 of 113.65μg/ml) or 48h (IC50 of 48.61μg/ml). Quercetin treatment also induced apoptosis via deregulating the expression of apoptotic genes, including Bax and Bcl-2, and arrested cell cycle at G2/M phases...
May 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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