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Mengnan Zhao, Fabienne Danhier, Chiara Bastiancich, Nicolas Joudiou, Lakshmi Pallavi Ganipineni, Nikolaos Tsakiris, Bernard Gallez, Anne des Rieux, Aleksandar Jankovski, John Bianco, Véronique Préat
Glioblastoma multiforme (GBM) is the most common primary malignant brain tumor. Despite available therapeutic options, the prognosis for patients with GBM remains very poor. We hypothesized that the intra-operative injection of a photopolymerizable hydrogel into the tumor resection cavity could sustain the release of the anti-cancer drug paclitaxel (PTX) encapsulated in poly (lactic-co-glycolic acid) (PLGA) nanoparticles and prevent GBM recurrence. The tumor was resected 13 days after implantation and a pre-gel solution composed of polyethylene glycol dimethacrylate (PEG-DMA) polymer, a photoinitiator and PTX-loaded PLGA nanoparticles (PTX PLGA-NPs) was injected into the tumor resection cavity...
July 16, 2018: International Journal of Pharmaceutics
Chen Xie, Meng Xu, Dejuan Lu, Weiguang Zhang, Laizang Wang, Hongwei Wang, Jianhua Li, Fubin Ren, Chao Wang
Glioma is the most common malignant brain tumor, and the incidence of glioma demonstrates an upward trend. It is vital to elucidate the pathogenesis of glioma and seek effective therapies. The aim of the present study was to identify the potential gene markers associated with glioma based on GSE31262 gene expression profiles, and to explore the underlying mechanism of glioma progression by analyzing the gene markers. The microarray dataset GSE31262 was downloaded and neural stem cell samples (control group) and glioma samples (glioma group) were analyzed to identify the differentially expressed genes (DEGs) between the two groups...
June 29, 2018: Molecular Medicine Reports
Bing Kong, Ming-Rong Zuo, Yan-Hui Liu
OBJECTIVE: To study the effect of cinobufagin (CB) on the proliferation inhibition and induction of apoptosis in glioblastoma cell lines U87 and its molecular mechanism. METHODS: A gradient concentration (0-20 μmol/L) of CB was used to treat the U87 glioma cells for 6 h,12 h,24 h and 48 h,respectively. Cell viabilities were determined by CCK-8 assay to discover the effects of different concentrations of CB on the proliferation of glioma cells. Different concentrations (1-20 μmol/L) of CB were used to treat the U87 glioma cells for 12 h and 24 h,hochest33342 staining assay was used to assess the apoptosis levels...
May 2018: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
Neetika Lal, Vishal Nemaysh, Pratibha Mehta Luthra
Recently, we have reported that Demethoxycurcumin induced Reactive oxygen species generation via inhibition of Mitochondrial Superoxide Dismutase is an initial event to trigger apoptosis through caspase-8 and 9 activation and to inhibit Akt/NF-κB survival signaling in human glioma U87 MG cells (R. Kumar, Lal, Nemaysh, & Luthra, 2018). Although cell-cycle disruption had been suggested to be the possible mechanism for DMC inhibitory effect on human glioma U87 MG cells, comprehensive mechanisms of cell-cycle arrest caused by DMC are not fully understood...
July 12, 2018: Toxicology and Applied Pharmacology
Muralidhar Allam, A K D Bhavani, Anwita Mudiraj, Nikhil Ranjan, Mallikarjuna Thippana, Phanithi Prakash Babu
A series of hybrid aza heterocycles containing pyrazolo[3,4-d]pyrimidin-4(5H)-ones tethered to 1,2,3-triazole scaffold were synthesized from 1,3-dipolar cycloaddition reaction of pyrazolopyrimidinone based alkyne with azides using Cu(II) catalyst in presence of sodium ascorbate and evaluated for their anticancer efficacy in vitro against C6 rat and U87 human glioma cell lines. These compounds induced a concentration dependent inhibition of C6 rat and U87 human glioma cell proliferation. Compound 5f arrested the cells at S-phase of the cell cycle and induced apoptosis in U87 GBM cell lines...
June 27, 2018: European Journal of Medicinal Chemistry
Farina Hanif, Kahkashan Perveen, Saima M Malhi, Huma Jawed, Shabana U Simjee
Glioblastoma Multiforme (GBM) is the most malignant and invasive tumor of the CNS. Although temozolomide (TMZ) has improved the survival, long-lasting responses have not been reported. Therefore, there is a need to develop improved treatments, one of which might be newly identified drugs which can be used in combination therapy with low doses of standard drugs. Verapamil (VP) a known antihypertensive drug has been shown to enhance the activity of bis-chloroethylnitrosourea (BCNU), a drug used to treat GBM. Since, TMZ has replaced BCNU as the standard GBM chemotherapy; therefore, we aimed to study in vitro interaction of VP and TMZ against GBM...
July 9, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Jun Chul Park, In Bok Chang, Jun Hyong Ahn, Ji Hee Kim, Joon Ho Song, Seung Myung Moon, Young-Han Park
Objective: Notch receptors are heterodimeric transmembrane proteins that regulate cell fate, such as differentiation, proliferation, and apoptosis. Dysregulated Notch pathway signaling has been observed in glioblastomas, as well as in other human malignancies. Nerve growth factor (NGF) is essential for cell growth and differentiation in the nervous system. Recent reports suggest that NGF stimulates glioblastoma proliferation. However, the relationship between NGF and Notch1 in glioblastomas remains unknown...
July 2018: Journal of Korean Neurosurgical Society
Ya-Jun Zhang, Zhi-Gang Xu, Shi-Qiang Li, Liu-Jun He, Yan Tang, Zhong-Zhu Chen, Dong-Lin Yang
Background: Recent studies showed that benzimidazoleisoquinolinone derivatives exhibit anticancer activity against human cancer cell lines. The aim of this study is to evaluate the anti-tumor effects and mechanisms of benzimidazoleisoquinolinones in isocitrate dehydrogenase-wildtype subtype of human glioblastoma (GBM) cells. Methods: Human U87 and LN229 cell lines were used to perform the experiments. MTT was applied to screen the effective small molecular inhibitors suppressing growth of GBM cells...
2018: Cancer Cell International
Tsuyoshi Fukushima, Makiko Kawaguchi, Koji Yamamoto, Fumiki Yamashita, Aya Izumi, Takashi Kaieda, Yuka Takezaki, Hiroshi Itoh, Hideo Takeshima, Hiroaki Kataoka
Hepatocyte growth factor activator inhibitor type 2 (HAI-2), encoded by the SPINT2 gene, is a membrane-anchored protein that inhibits proteases involved in the activation of hepatocyte growth factor (HGF), a ligand of MET receptor. Epigenetic silencing of the SPINT2 gene has been reported in a human glioblastoma cell line (U87) and glioblastoma-derived cancer stem cells. However, the incidence of SPINT2 methylation in tumor tissues obtained from glioma patients is unknown. In this study, we analyzed the methylation status of the SPINT2 gene of eight human glioblastoma cell lines and surgically resected glioma tissues of different grades (II, III and IV) by bisulfite sequence analysis and methylation-specific PCR (MSP)...
July 10, 2018: Cancer Science
Fei Liu, Xiaoxia Xu, Hua Zhu, Yan Zhang, Jianhua Yang, Lifang Zhang, Nan Li, Lin Zhu, Hank F Kung, Zhi Yang
Sustaining the growth of tumor cells requires extra energy and metabolic building blocks. In addition to consuming glucose, glutamine may play the role as an alternative source of nutrient for growth and survival. We aim to characterize, a glutamine analog, 18F-(2S,4R)4-fluoroglutamine (18F-(2S,4R)4-FGln), as an imaging agent for interrogating the role of glutamine from in vitro study of tumor cells to clinical manifestation in breast cancer patients. Purity was measured by radio-high performance liquid chromatography (Radio-HPLC), and the stability after production was evaluated in phosphate buffer saline (PBS), saline, mouse and human serum buffers...
July 9, 2018: Molecular Pharmaceutics
Chuanhong Zhong, Yitian Chen, Bei Tao, Lilei Peng, Tangming Peng, Xiaobo Yang, Xiangguo Xia, Ligang Chen
BACKGROUND: LIM and SH3 protein 1 (LASP1) is upregulated in several types of human cancer and implicated in cancer progression. However, the expression and intrinsic function of LASP1 in glioblastoma (GBM) remains unclear. METHOD: Oncomine and The Cancer Genome Atlas (TCGA) database was analyzed for the expression and clinical significance of LASP1 in GBM. LASP1 mRNA and protein level were measured by qRT-PCR and western blotting. The effect of LASP1 on GBM proliferation was examined by MTT assay and colony formation assay, the effect of LASP1 on sensitivity of Temozolomide was measured by flow cytometry and subcutaneous tumor model...
July 6, 2018: BMC Cancer
Xiaofeng Chen, Weiping Kuang, Hongxing Huang, Bo Li, Yong Zhu, Bin Zhou, Lin Yan
Glioblastoma is the most common and malignant primary brain tumor. RWD domain containing 3 (RWDD3) has been previously reported to serve a promoting role in pituitary tumors. However, the exact role of RWDD3 in glioblastoma remains unclear. Therefore, the present study aimed to investigate the expression levels of RWDD3 in human glioblastoma tissues and cell lines, as well as to examine the regulatory mechanism of RWDD3 underlying glioblastoma growth and metastasis. The results revealed that RWDD3 was significantly upregulated in glioblastoma tissues compared with normal brain tissues, while high expression of RWDD3 was associated with a shorter survival time of glioblastoma patients...
July 2018: Experimental and Therapeutic Medicine
Wan Li, Jinyi Liu, Weiqi Fu, Xiangjin Zheng, Liwen Ren, Shiwei Liu, Jinhua Wang, Tengfei Ji, Guanhua Du
BACKGROUND: Glioblastoma (GBM) is the most common, malignant, and lethal primary brain tumor in adults accounting for about 50% of all gliomas. Up to now, the chemotherapy approaches for GBM were limited. 3-O-acetyl-11-keto-β-boswellic acid (AKBA), the major active ingredient of the gum resin from Boswellia serrata and Boswellia carteri Birdw., was reported to inhibit the growth of many types of cancer cells; however, the underlying mechanism of its anticancer effects are still unclear...
July 3, 2018: Journal of Experimental & Clinical Cancer Research: CR
Ran Kim, Seokyeon Lee, Jihyun Lee, Minji Kim, Won Jung Kim, Hee Won Lee, Min Young Lee, Jongmin Kim, Woochul Chang
Exosomes are small membranous vesicles which contain abundant RNA molecules, and are transferred from releasing cells to uptaking cells. MicroRNA (miRNA) is one of the transferred molecules affecting the adopted cells, including glioma cells. We hypothesized that mesenchymal stem cells (MSCs) can secrete exosomes loading miRNA and have important effects on the progress of gliomas. To determine these effects by treating exosomal miRNA in culture media of miRNA mimic transfected MSCs, we assessed the in vitro cell proliferation and invasion capabilities, and the expression level of relative proteins associated with cell apoptosis, growth and migration...
July 3, 2018: BMB Reports
Kacper A Walentynowicz, Natalia Ochocka, Maria Pasierbinska, Kamil Wojnicki, Karolina Stepniak, Jakub Mieczkowski, Iwona A Ciechomska, Bozena Kaminska
Immune cells accumulating in the microenvironment of malignant tumors are tumor educated and contribute to its growth, progression, and evasion of antitumor immune responses. Glioblastoma (GBM), the common and most malignant primary brain tumor in adults, shows considerable accumulation of resident microglia and peripheral macrophages, and their polarization into tumor-supporting cells. There are controversies regarding a functional phenotype of glioma-associated microglia/macrophages (GAMs) due to a lack of consistent markers...
2018: Frontiers in Immunology
Hye Hyeon Yun, Ji-Ye Baek, Gwanwoo Seo, Yong Sam Kim, Jeong-Heon Ko, Jeong-Hwa Lee
The expression of BCL-2 interacting cell death suppressor (BIS), an anti-stress or anti-apoptotic protein, has been shown to be regulated at the transcriptional level by heat shock factor 1 (HSF1) upon various stresses. Recently, HSF1 was also shown to bind to BIS, but the significance of these protein-protein interactions on HSF1 activity has not been fully defined. In the present study, we observed that complete depletion of BIS using a CRISPR/Cas9 system in A549 non-small cell lung cancer did not affect the induction of heat shock protein (HSP) 70 and HSP27 mRNAs under various stress conditions such as heat shock, proteotoxic stress, and oxidative stress...
July 2018: Korean Journal of Physiology & Pharmacology
Xiaoyan Wu, Huaqian Dong, Xiang Ye, Li Zhong, Tiantian Cao, Qiping Xu, Jun Wang, Yu Zhang, Jinhong Xu, Wei Wang, Qiang Wei, Ying Liu, Shuhui Wang, Yiming Shao, Huiqin Xing
The human immunodeficiency virus-1 (HIV-1) regulatory protein Tat plays an important role during HIV-1-associated neurocognitive disorders (HAND) by inducing neuronal autophagy. In this study, we used immunohistochemistry, immunofluorescence, western blot, qRT-PCR, and RNA interference to elucidate the involvement of Bcl-2-associated athanogene 3 (BAG3) in the pathogenesis of HIV-1 Tat-induced autophagy during HAND. We found that BAG3 expression is elevated in astrocytes in frontal cortex of macaques infected with simian immunodeficiency virus-human immunodeficiency chimeric virus (SHIV)...
July 2, 2018: Cell Cycle
Wei-Qiang Jia, Zhao-Tao Wang, Ming-Ming Zou, Jian-Hao Lin, Ye-Hai Li, Liang Zhang, Ru-Xiang Xu
BACKGROUND/AIMS: As a natural antioxidant, verbascoside (VB) is proved to be a promising method for the treatment of oxidative-stress-related neurodegenerative diseases. Thus, this study aimed to investigate the effects of VB on glioblastoma cell proliferation, apoptosis, migration, and invasion as well as the mechanism involving signal transducer and activator of transcription 3 (STAT3) and Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP-1). METHODS: U87 cells were assigned to different treatments...
June 29, 2018: Cellular Physiology and Biochemistry
Liang Zhang, Yidong Cao, Minghao Wei, Xue Jiang, Dong Jia
Glioblastoma (GBM) is a devastating and highly aggressive tumor, which is apoptosis resistant and difficult to cure. Recently, long noncoding RNAs have been shown to play a pivotal role in GBM progression. Evidence has suggested that retinal noncoding RNA3 (RNCR3) is a GBM-associated noncoding RNA and is under-expressed in GBM. However, the function and mechanism of RNCR3 on GBM cell growth and apoptosis are still uncertain. In the current study, we found that the level of RNCR3 is decreased in U87, U251, U373, and A172 GBM cell lines when compared with the normal human astrocytes...
June 28, 2018: Journal of Cellular Biochemistry
Thatchawan Thanasupawat, Aleksandra Glogowska, Maxwell Burg, Jerry Krcek, Jason Beiko, Marshall Pitz, Guo-Jun Zhang, Sabine Hombach-Klonisch, Thomas Klonisch
The C1Q-TNF related peptide CTRP8 has recently emerged as a novel ligand of the G protein coupled receptor RXFP1 in the fatal brain tumor glioblastoma (GBM). We previously demonstrated that the CTRP8-RXFP1 ligand-receptor system promotes motility and matrix invasion of patient GBM and U87 MG cells by specific phosphorylation of PI-3-kinase and protein kinase C. Here, we demonstrate a novel role for CTRP8 in protecting human GBM cells against the DNA alkylating damage of temozolomide (TMZ), the standard chemotherapy drug used to treat GBM...
June 27, 2018: Molecular Oncology
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