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https://www.readbyqxmd.com/read/28096969/dendrosomal-nanocurcumin-and-p53-overexpression-synergistically-trigger-apoptosis-in-glioblastoma-cells
#1
Reihaneh Keshavarz, Babak Bakhshinejad, Sadegh Babashah, Narges Baghi, Majid Sadeghizadeh
OBJECTIVES: Glioblastoma is the most lethal tumor of the central nervous system. Here, we aimed to evaluate the effects of exogenous delivery of p53 and a nanoformulation of curcumin called dendrosomal curcumin (DNC), alone and in combination, on glioblastoma tumor cells. MATERIALS AND METHODS: MTT assay was exploited to measure the viability of U87-MG cells against DNC treatment. Cells were separately subjected to DNC treatment and transfected with p53-containing vector and then were co-exposed to DNC and p53 overexpression[A GA1][B2]...
December 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28088078/genetically-tailored-magnetosomes-used-as-mri-probe-for-molecular-imaging-of-brain-tumor
#2
M Boucher, F Geffroy, S Prévéral, L Bellanger, E Selingue, G Adryanczyk-Perrier, M Péan, C T Lefèvre, D Pignol, N Ginet, S Mériaux
We investigate here the potential of single step production of genetically engineered magnetosomes, bacterial biogenic iron-oxide nanoparticles embedded in a lipid vesicle, as a new tailorable magnetic resonance molecular imaging probe. We demonstrate in vitro the specific binding and the significant internalization into U87 cells of magnetosomes decorated with RGD peptide. After injection at the tail vein of glioblastoma-bearing mice, we evidence in the first 2 h the rapid accumulation of both unlabeled and functionalized magnetosomes inside the tumor by Enhanced Permeability and Retention effects...
December 19, 2016: Biomaterials
https://www.readbyqxmd.com/read/28081256/inhibition-of-acetyl-coa-carboxylase-1-acc1-and-2-acc2-reduces-proliferation-and-de-novo-lipogenesis-of-egfrviii-human-glioblastoma-cells
#3
Jessica E C Jones, William P Esler, Rushi Patel, Adhiraj Lanba, Nicholas B Vera, Jeffrey A Pfefferkorn, Cecile Vernochet
Tumor cell proliferation and migration processes are regulated by multiple metabolic pathways including glycolysis and de novo lipogenesis. Since acetyl-CoA carboxylase (ACC) is at the junction of lipids synthesis and oxidative metabolic pathways, we investigated whether use of a dual ACC inhibitor would provide a potential therapy against certain lipogenic cancers. The impact of dual ACC1/ACC2 inhibition was investigated using a dual ACC1/ACC2 inhibitor as well as dual siRNA knock down on the cellular viability and metabolism of two glioblastoma multiform cancer cell lines, U87 and a more aggressive form, U87 EGFRvIII...
2017: PloS One
https://www.readbyqxmd.com/read/28076826/identification-of-new-pyrrolo-2-3-d-pyrimidines-as-src-tyrosine-kinase-inhibitors-in%C3%A2-vitro-active-against-glioblastoma
#4
Francesca Musumeci, Anna Lucia Fallacara, Chiara Brullo, Giancarlo Grossi, Lorenzo Botta, Pierpaolo Calandro, Mario Chiariello, Miroslava Kissova, Emmanuele Crespan, Giovanni Maga, Silvia Schenone
In the last few years, several pyrrolo-pyrimidine derivatives have been either approved by the US FDA and in other countries for the treatment of different diseases or are currently in phase I/II clinical trials. Herein we present the synthesis and the characterization of a novel series of pyrrolo[2,3-d]pyrimidines, compounds 8a-j, and their activity against Glioblastoma multiforme (GBM). Docking studies and MM-GBSA analysis revealed the ability of such compounds to efficiently interact with the ATP binding site of Src...
December 19, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28075458/silencing-of-forkhead-box-d1-inhibits-proliferation-and-migration-in-glioma-cells
#5
Yuan-Feng Gao, Tao Zhu, Xiao-Yuan Mao, Chen-Xue Mao, Ling Li, Ji-Ye Yin, Hong-Hao Zhou, Zhao-Qian Liu
Despite the extensive role of Forkhead box transcription factors in the development and progression of various cancers, little is known about their role in glioma. We examined the expression and function of Forkhead box D1 (FOXD1) in glioma cell behavior and found that FOXD1 was upregulated and directly correlated with the glioma grade. Data analysis also revealed significant differences in FOXD1 expression for both gene expression profiles (GSE4290 and GSE7696) and the TCGA datasets. Additionally, decreased FOXD1 expression in U251 and U87 glioma cells caused a delay in cell growth and a disruption in colony formation...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/28068630/potential-mechanisms-of-microrna-129-5p-in-inhibiting-cell-processes-including-viability-proliferation-migration-and-invasiveness-of-glioblastoma-cells-u87-through-targeting-fndc3b
#6
Haisong Xu, Yanxia Hu, Wusi Qiu
The purpose of our study is to clarify the effects of microRNA-129-5p (miR-129-5p) in cellular processes correlated with cancer development and progression of Glioblastoma (GBM) cell by regulating FNDC3B. MiR-129-5p and FNDC3B expression in GBM tissues and tumor adjacent tissues were tested by quantitative real-time PCR. We validated the target relationship between miR-129-5p and FNDC3B by dual luciferase reporter gene system. MTT, colony formation, flow cytometry, Transwell and wound healing assays were used to analyze cell viability, proliferation, apoptosis, invasiveness and migration...
January 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28061441/activation-of-trpm7-by-naltriben-enhances-migration-and-invasion-of-glioblastoma-cells
#7
Raymond Wong, Ekaterina Turlova, Zhong-Ping Feng, James T Rutka, Hong-Shuo Sun
Glioblastoma (GBM), the most common and aggressive brain tumor in the central nervous system, remains a lethal diagnosis with a median survival of < 15 months. Aberrant expression of the TRPM7 channel has been linked to GBM functions. In this study, using the human GBM cell line U87, we evaluated the TRPM7 activator naltriben on GBM viability, migration, and invasiveness. First, using the whole-cell patch-clamp technique, we showed that naltriben enhanced the endogenous TRPM7-like current in U87 cells. In addition, with Fura-2 Ca2+ imaging, we observed robust Ca2+ influx following naltriben application...
January 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28053545/high-expression-of-long-noncoding-rna-hulc-is-a-poor-predictor-of-prognosis-and-regulates-cell-proliferation-in-glioma
#8
Hong Yan, Rui Tian, Min Zhang, Jing Wu, Min Ding, Jie He
PURPOSE: Emerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. This study investigated the role of lncRNA highly upregulated in liver cancer (HULC) expression in glioma and its clinical significance in glioma patients. MATERIALS AND METHODS: HULC expression was detected in glioma tissues and cell lines by using real-time quantitative reverse transcription polymerase chain reactions. Association between HULC levels and clinicopathological factors and patients prognosis was also analyzed...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28053137/activation-of-cecr1-in-m2-like-tams-promotes-paracrine-stimulation-mediated-glial-tumor-progression
#9
Changbin Zhu, Dana Mustafa, Ping-Pin Zheng, Marcel van der Weiden, Andrea Sacchetti, Maarten Brandt, Ihsan Chrifi, Dennie Tempel, Pieter J M Leenen, Dirk Jan Duncker, Caroline Cheng, Johan M Kros
BACKGROUND: The majority of glioma-associated microglia/macrophages have been identified as M2-type macrophages with immune suppressive and tumor supportive action. Recently, the extracellular adenosine deaminase protein Cat Eye Syndrome Critical Region Protein 1 (CECR1) was shown to regulate macrophage maturation. In this study, we investigate the role of CECR1 in the regulation of the glioma-associated macrophage response. METHODS: Expression of CECR1 was assessed in human glioma samples...
January 3, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28047779/we-h-207a-08-characterization-of-a-broad-spectrum-cancer-targeted-mri-contrast-agent
#10
C Brunnquell, R Zhang, A Pinchuk, J Weichert
PURPOSE: To characterize the relaxation properties and tumor targeting capabilities of a novel alkylphosphocholine (APC) analog MR contrast agent, Gd-DO3A-404. METHODS: Relaxivities were measured via T1 and T2 mapping of Gd-DO3A-404 with inversion recovery and spin echo pulse sequences, respectively. Uptake was characterized in flank xenograft models of non-small cell lung cancer (A549) and glioma (U87) and compared with uptake of Dotarem. Mice (N=3 per model per agent) were delivered 2...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28035411/ellagic-acid-inhibits-human-glioblastoma-growth-in%C3%A2-vitro-and-in%C3%A2-vivo
#11
Dongliang Wang, Qianxue Chen, Yinqiu Tan, Baohui Liu, Chao Liu
Ellagic acid (EA) is present in various fruits and plants and has recently been found to possess anticarcinogenic effects. The objective of this study was to investigate the anti‑glioblastoma effect of EA and its mechanisms in vitro and in vivo. We first studied the anticancer activity of EA in U87 and U118 human glioblastoma cell lines. The cell viability and cell proliferation were examined by Cell Counting Kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine staining respectively. The cell cycle was detected with propidium iodide staining method by flow cytometry and the DNA damage of the cells caused by EA exposure was evaluated by detection of γ-H2AX foci...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/28031711/synergistic-anti-glioma-effect-of-a-coloaded-nano-drug-delivery-system
#12
Huae Xu, Feng Jia, Pankaj Kumar Singh, Shu Ruan, Hao Zhang, Xiaolin Li
The anti-glioma effect of temozolomide (Tem) is sometimes undermined by the emerging resistance. Recently, resveratrol (Res), herbal medicine extracted from grape seeds, has been demonstrated for its potential use in chemosensitization. In the current study, both these drugs were loaded simultaneously into nanoparticles with methoxy poly(ethylene glycol)-poly epsilon caprolactone (mPEG-PCL) as drug carriers in order to achieve better antitumor efficiency. Tem/Res-coloaded mPEG-PCL nanoparticles were constructed, characterized, and tested for antitumor effect on glioma cells by using in vitro and xenograft model system...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28031240/combination-of-a-stat3-inhibitor-and-an-mtor-inhibitor-against-a-temozolomide-resistant-glioblastoma-cell-line
#13
Haruo Miyata, Tadashi Ashizawa, Akira Iizuka, Ryota Kondou, Chizu Nonomura, Takashi Sugino, Kenichi Urakami, Akira Asai, Nakamasa Hayashi, Koichi Mitsuya, Yoko Nakasu, Ken Yamaguchi, Yasuto Akiyama
BACKGROUND: Temozolomide-resistant (TMZ-R) glioblastoma is very difficult to treat, and a novel approach to overcome resistance is needed. MATERIALS AND METHODS: The efficacy of a combination treatment of STAT3 inhibitor, STX-0119, with rapamycin was investigated against our established TMZ-resistant U87 cell line. RESULTS: The growth-inhibitory effect of the combination treatment was significant against the TMZ-R U87 cell line (IC50: 78 μM for STX-0119, 30...
2, 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28029227/physicochemical-antioxidant-and-cytotoxic-properties-of-xao-tam-phan-paramignya-trimera-root-extract-and-its-fractions
#14
Nguyen Van Tang, Jennette A Sakoff, Christopher J Scarlett
Xao tam phan (Paramignya trimera (Oliv.) Guillaum) has been used as a medicinal plant for cancer prevention and treatment in recent years. The objective of this study was to determine the physicochemical, antioxidant and cytotoxic properties of crude P. trimera root (PTR) extract and its fractions using methanol as a solvent and microwave-assisted extraction as an advanced technique for preparation of the PTR extract. The results showed that the PTR extract had high contents of saponins, phenolics, flavonoids and proanthocyanidins (7731...
December 28, 2016: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28012197/interstitial-photodynamic-therapy-and-glioblastoma-light-fractionation-in-a-preclinical-model
#15
Henri-Arthur Leroy, Maximilien Vermandel, Anne-Sophie Vignion-Dewalle, Bertrand Leroux, Claude-Alain Maurage, Alain Duhamel, Serge Mordon, Nicolas Reyns
BACKGROUND: Glioblastoma is a high-grade cerebral tumor with local recurrence and poor outcome. Photodynamic therapy (PDT) is a localized treatment based on the light activation of a photosensitizer (PS) in the presence of oxygen, which results in the formation of cytotoxic species. The delivery of fractionated light may enhance treatment efficacy by reoxygenating tissues. OBJECTIVE: To evaluate the efficiency of two light-fractionation schemes using immunohistological data...
December 24, 2016: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28011394/-131-i-labeled-and-dox-loaded-multifunctional-nanoliposomes-for-radiotherapy-and-chemotherapy-in-brain-gliomas
#16
REVIEW
Jingmei Gao, Lei Fang, Danyang Sun, Yiming Shen, Yiming Hu, Ning Li, Jin Chang, Wei Li, Jian Tan
The codelivery of different therapeutics is a promising option because of its synergetic effects of drugs. In this study, a new combination therapy that used the doxorubicin-loaded and (131)I-labeled nanoliposomes ((131)I-DOX-NL) was proposed to delay tumor growth of gliomas, which are characterized by significant mortality and morbidity. (131)I-DOX-NL was constructed based on bovine serum albumin (BSA)-tailor made hydrophobic maleimide-functionalized poly(ε-caprolactone) (PCL) (Fig. 1) and was evaluated by cellular viability in vitro and by U87 xenograft models in vivo...
December 20, 2016: Brain Research
https://www.readbyqxmd.com/read/28004979/epigenetic-silencing-of-the-human-18%C3%A2-kda-translocator-protein-in-a-t-cell-leukemia-cell-line
#17
Ryan J Middleton, Winnie Wai-Ying Kam, Guo-Jun Liu, Richard B Banati
The mitochondrial membrane 18 kDa translocator protein (TSPO), previously known as the peripheral benzodiazepine receptor, is constitutively expressed in most organs, most abundantly in hormonal tissue and cells of mononuclear phagocyte lineage, while in the brain, TSPO expression is induced in the wake of injury, inflammation, and neurodegeneration. Increased TSPO expression is also prominent in several cancerous tissues where it appears to correlate with the degree of malignancy. Currently, TSPO is thus actively investigated as a generic biomarker for disease activity and a therapeutic target for a wide range of diseases...
December 22, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/28004224/antioxidant-and-antiproliferative-effects-of-different-solvent-fractions-from-terminalia-belerica-roxb-fruit-on-various-cancer-cells
#18
Tapasree Basu, Sourav Panja, Nikhil Baban Ghate, Dipankar Chaudhuri, Nripendranath Mandal
Terminalia belerica Roxb. fruits have been previously reported against diabetes, ulcer, microbial problems and hepatotoxicity. The present study was aimed to investigate antioxidant and anticancer potential of sequentially fractionated hexane (TBHE), chloroform (TBCE), ethyl acetate (TBEE), butanol (TBBE) and water (TBWE) extracts from the 70% methanolic extract of T. belerica fruits. TBCE, TBEE, TBBE and TBWE showed excellent ROS (reactive oxygen species) and RNS (reactive nitrogen species) scavenging activities which was investigated using 11 different assays for various free radicals...
December 21, 2016: Cytotechnology
https://www.readbyqxmd.com/read/28000842/the-anti-angiogenic-role-of-soluble-form-vegf-receptor-in-malignant-gliomas
#19
Shingo Takano, Eiichi Ishikawa, Masahide Matsuda, Noriaki Sakamoto, Hiroyoshi Akutsu, Tetsuya Yamamoto, Akira Matsumura
Anti-angiogenic therapy represents a promising, new therapeutic modality for malignant gliomas. The present study was designed to define the malignant glioma cases most suitable for anti-angiogenic therapy and to demonstrate the efficacy of anti-angiogenic therapy using soluble-form Flt1 (sFlt1) gene delivery in mice. In human malignant glioma samples (39 glioblastomas, 21 anaplastic astrocytomas and 4 anaplastic oligoastrocytomas), protein expression of VEGF, and its specific natural inhibitor, sFlt1, as well as vessel architecture were assessed...
February 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28000106/knockdown-of-nupr1-inhibits-the-proliferation-of-glioblastoma-cells-via-erk1-2-p38-mapk-and-caspase-3
#20
Jun Li, Siyang Ren, Yongjian Liu, Zhigang Lian, Bin Dong, Yiqun Yao, Yinghui Xu
Nuclear protein-1 (NUPR1), located on chromosome 16p11.2, is a stress response factor that plays an important role in the growth and migration of human malignant tumor cells. However, the role of NUPR1 in glioblastoma remains poorly understood. The expression level of NUPR1 was detected by quantitative real-time PCR and immunohistochemistry (IHC). Wound healing, MTT, cell counting and BrdU assays were used to analyze the migration and proliferation of glioblastoma cells after down-regulating NUPR1 expression using a lentiviral vector...
December 20, 2016: Journal of Neuro-oncology
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