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https://www.readbyqxmd.com/read/29156770/sco-spondin-oligopeptide-inhibits-angiogenesis-in-glioblastoma
#1
Romain Bibes, Stéphane Gobron, François Vincent, Carole Mélin, Nicolas Vedrenne, Aurélie Perraud, Francois Labrousse, Marie-Odile Jauberteau, Fabrice Lalloué
Angiogenesis plays a critical role in glioblastoma growth and progression. We therefore aimed at evaluating the anti-angiogenic properties of an oligopeptide originating from SCO-spondin (NX) on a model of human glioblastoma. To this end, we studied the impact of NX treatment on human brain endothelial cells (HBMECs) alone or co-cultured with glioblastoma cells (U87-MG) on apoptosis, proliferation, migration and release of angiogenic factors. We further investigated the anti-angiogenic potential of NX on human glioblastoma cells grown on chorio-allantoic membrane (CAM) or in glioblastoma xenografts...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156717/oligomer-procyanidins-f2-repress-hif-1%C3%AE-expression-in-human-u87-glioma-cells-by-inhibiting-the-egfr-akt-mtor-and-mapk-erk1-2-signaling-pathways-in-vitro-and-in-vivo
#2
Hong-Li Zheng, Li-Hui Wang, Bao-Shan Sun, Yi Li, Jing-Yu Yang, Chun-Fu Wu
Hypoxia-inducible factor-1 (HIF-1) is over-expressed in gliomas and has become one of the most compelling tumor targets. In this study, we found that oligomer procyanidins (F2) can suppress the expressions of HIF-1α and its target genes in U87 cells, and also down-regulate the EGFR/PI3K/AKT/mTOR and MAPK/ERK1/2 pathways in vitro and in vivo. Furthermore, hypoxia-induced formation of tubular structures by human umbilical vascular endothelial cells and the migration and invasion of U87 cells could be inhibited by F2 in a HIF-1 dependent manner...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156346/abcg2-mediated-suppression-of-chlorin-e6-accumulation-and-photodynamic-therapy-efficiency-in-glioblastoma-cell-lines-can-be-reversed-by-ko143
#3
Sara A Abdel Gaber, Patricia Müller, Wolfgang Zimmermann, Dirk Hüttenberger, Rainer Wittig, Mahmoud H Abdel Kader, Herbert Stepp
BACKGROUND: Photodynamic therapy (PDT) of malignant brain tumors is a promising adjunct to standard treatment, especially if tumor stem cells thought to be responsible for tumor progression and therapy resistance were also susceptible to this kind of treatment. However, some photosensitizers have been reported to be substrates of ABCG2, one of the membrane transporters mediating resistance to chemotherapy. Here we investigate, whether inhibition of ABCG2 can restore sensitivity to photosensitizer chlorin e6-mediated PDT...
October 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29145888/tgf-%C3%AE-2-initiates-autophagy-via-smad-and-non-smad-pathway-to-promote-glioma-cells-invasion
#4
Chao Zhang, Xin Zhang, Ran Xu, Bin Huang, An-Jing Chen, Chao Li, Jian Wang, Xin-Gang Li
BACKGROUND: Glioblastoma multiforme (GBM) is characterized by lethal aggressiveness and patients with GBM are in urgent need for new therapeutic avenues to improve quality of life. Current studies on tumor invasion focused on roles of cytokines in tumor microenvironment and numerous evidence suggests that TGF-β2 is abundant in glioma microenvironment and vital for glioma invasion. Autopagy is also emerging as a critical factor in aggressive behaviors of cancer cells; however, the relationship between TGF-β2 and autophagy in glioma has been poorly understood...
November 16, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29143921/dichloroacetate-induced-intracellular-acidification-in-glioblastoma-in-vivo-detection-using-aacid-cest-mri-at-9-4%C3%A2-tesla
#5
Mohammed Albatany, Alex Li, Susan Meakin, Robert Bartha
Intracellular pH (pHi) plays an important role in the maintenance of normal cell function, and is maintained within a narrow range by the activity of transporters located at the plasma membrane. Modulation of tumor pHi may influence proliferation, apoptosis, chemotherapy resistance, and thermosensitivity. Chemical exchange saturation transfer (CEST) is a novel MRI contrast mechanism that is dependent on cellular pH. Amine and amide concentration-independent detection (AACID) is a recently developed CEST contrast method that is intracellular pH (pHi) weighted...
November 16, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29138027/imidazo-1-2-a-pyridines-linked-with-thiazoles-thiophene-motif-through-keto-spacer-as-potential-cytotoxic-agents-and-nf-%C3%AE%C2%BAb-inhibitors
#6
Kamala K Vasu, Chander Singh Digwal, Amit N Pandya, Dhaivat H Pandya, Jayesh A Sharma, Sneha Patel, Milee Agarwal
A series of new imidazo[1,2-a]pyridine linked with thiazole/thiophene motif through a keto spacer were synthesized and tested for their cytotoxic potential against three human cancer cell lines including A549, HeLa and U87-MG using MTT assay. Compounds A2, A3, A4, C1 and C2 showed cytotoxicity against all the three cell lines. The selectivity index for compound A4 for A549 and HeLa cells was comparable to that of doxorubicin. Among the synthesized compounds, B5 showed the maximum inhibition of NF-κB activity as ascertained by NF-κB reporter assay (IC50 = 6...
November 11, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29131445/airyscan-super-resolution-microscopy-of-mitochondrial-morphology-and-dynamics-in-living-tumor-cells
#7
Vladimir L Kolossov, Mayandi Sivaguru, Joseph Huff, Katherine Luby, Kaviamuthan Kanakaraju, H Rex Gaskins
Mitochondrial morphology is regulated by continuous fusion-and-fission events that are essential for maintaining normal function. Despite the prominence of mitochondrial function in energy generation and cell signaling, understanding of processes of fusion and fission dynamics has been hampered by the lack of high-resolution optical systems that accommodate live-cell imaging. We have examined different confocal modalities in terms of resolution and signal-to-noise ratio (SNR) in a point scanning confocal microscope with Airyscan super-resolution (AS-SR)...
November 13, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29129044/anti-sema3a-antibody-a-novel-therapeutic-agent-to-suppress-gbm-tumor-growth
#8
Jaehyun Lee, Yong Jae Shin, Kyoungmin Lee, Hee Jin Cho, Jason K Sa, Sang-Yun Lee, Seok-Hyung Kim, Jeongwu Lee, Yeup Yoon, Do-Hyun Nam
Purpose: Glioblastoma (GBM) is classified as one of the most aggressive and lethal brain tumor. Great strides have been made in understanding the genomic and molecular underpinnings of GBM, which translated into development of new therapeutic approaches to combat such deadly disease. However, there are only few therapeutic agents that can effectively inhibit GBM invasion in a clinical framework. In an effort to address such challenges, we have generated anti-SEMA3A monoclonal antibody as a potential therapeutic antibody against GBM progression...
November 10, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/29118922/il-6-increases-sdcbp-expression-cell-proliferation-and-cell-invasion-by-activating-jak2-stat3-in-human-glioma-cells
#9
Fang Cao, Qiang Zhang, Wei Chen, Chong Han, Yang He, Qishan Ran, Shengtao Yao
Syndecan-binding protein (SDCBP), which is induced by tumor necrosis factor-α and interferon-γ, controls the proliferation and invasion of several different types of cancer cells. Interleukin-6 (IL-6) is known to play an important role in the glioma cell growth and invasion. The present study aimed to investigate the relationship between IL-6 and SDCBP in glioma cells. SDCBP expression was knocked down in two glioma cell lines (T98G and U87) by small interfering RNA (siRNA) transfection. Cell proliferation and invasion were significantly repressed following SDCBP knockdown, and there was a positive correlation between SDCBP and IL-6 expression levels in glioma tissues...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29118089/oncolytic-virotherapy-blockade-by-microglia-and-macrophages-requires-stat1-3
#10
Zahid M Delwar, Yvonne Kuo, Yan H Wen, Paul S Rennie, William Jia
The first oncolytic virotherapy employing HSV-1 (oHSV-1) was approved recently by the FDA to treat cancer, but further improvements in efficacy are needed to eradicate challenging refractory tumors such as glioblastomas (GBM). Microglia/macrophages comprising ~40% of a GBM tumor may limit virotherapeutic efficacy. Here we show these cells suppress oHSV-1 growth in gliomas by internalizing the virus through phagocytosis. Internalized virus remained capable of expressing reporter genes while viral replication was blocked...
November 8, 2017: Cancer Research
https://www.readbyqxmd.com/read/29117732/phytochemical-constituents-from-dietary-plant-citrus-hystrix
#11
Mathusalini Sadasivam, Chandraprakash Kumarasamy, Arasakumar Thangaraj, Mani Govindan, Gayathri Kasirajan, Viswanathan Vijayan, Velmurugan Devadasan, Lin Chia-Her, Gogul Ramnath Madhusudhanan, Thirugnanasampandan Ramaraj, Mohan Palathurai Subramaniam
Chemical investigation of the fruit peel of dietary plant Citrus hystrix offered two new flavones 5,6,4'-trihydroxypyranoflavone I and 5,4'-dimethyl-6-prenylpyranoflavone XIII besides 11 known compounds. The structures of all compounds were elucidated with the aid of suitable analytical methods like 1D, 2D-NMR, mass and single crystal X-ray analysis. An X-ray crystal study of compound II was done for the first time and the compounds I-VI, XI and XII are hitherto not reported from this plant. Biological studies revealed that compound I found to have a good antidiabetic and antiacetylcholinesterase activities meanwhile compounds II, III and V showed a significant free radical scavenging ability as well as antioxidant capacity...
November 9, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29115617/three-dimensional-hydrogel-is-suitable-for-targeted-investigation-of-amoeboid-migration-of-glioma-cells
#12
Yubao Huang, Luqing Tong, Li Yi, Chen Zhang, Long Hai, Tao Li, Shengping Yu, Wei Wang, Zhennan Tao, Haiwen Ma, Peidong Liu, Yang Xie, Xuejun Yang
Glioblastoma (GBM) invasion and migration are key biological behaviors leading to refractoriness to current therapies and infiltration into the non‑tumor brain parenchyma. GBM cell migration is strongly dependent on tumor architecture in vivo, which is absent in traditional two‑dimensional (2D) monolayer culture. The present study applied a three‑dimensional (3D) hydrogel model to rebuild the tumor architecture in vitro. Treatment with NSC23766, a specific inhibitor of Ras‑related C3 botulinum toxin substrate 1 (Rac1), inhibited the mesenchymal invasiveness however triggered the amoeboid motility called mesenchymal‑amoeboid transition (MAT)...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115581/tramadol-attenuates-the-sensitivity-of-glioblastoma-to-temozolomide-through-the-suppression-of-cx43%C3%A2-mediated-gap-junction-intercellular-communication
#13
Lingzhi Wang, Yuexia Peng, Jianxin Peng, Min Shao, Li Ma, Zhuoli Zhu, Guocheng Zhong, Zhengyuan Xia, Huansen Huang
Analgesics and antineoplastic drugs are often used concurrently for cancer patients. Our previous study reported that gap junctions composed of connexin32 (Cx32) was implicated in the effect of analgesics on cisplatin cytotoxicity. However, the effect of analgesic on the most widely expressed connexin (Cx), connexin43 (Cx43), and whether such effect mediates the influence on chemotherapeutic efficiency remain unknown. By manipulation of Cx43 expression or gap junction function, we found that there were gap junction-dependent and independent effect of Cx43 on temozolomide (TMZ) sensitivity in U87 glioblastoma cells...
November 3, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29115572/talens-mediated-homozygous-ccr5%C3%AE-32-mutations-endow-cd4-u87-cells-with-resistance-against-hiv%C3%A2-1-infection
#14
Ai Qing Yu, Yan Ding, Zhi Yong Lu, Yan Zhe Hao, Zhi Ping Teng, Shi Rong Yan, Dong Sheng Li, Yi Zeng
Since evidence suggests that transplantation of bone marrow stem cells with the C‑C chemokine receptor type 5 (CCR5)Δ32/Δ32 genotype may cure patients infected with human immunodeficiency virus (HIV)‑1, the present study aimed to reproduce the CCR5Δ32 mutation in cluster of differentiation (CD)4+ U87 cells using genome engineering methods. A modified transcription activator‑like effector nucleases (TALENs) technique, combined with homologous recombination for site‑specific, size‑controlled and homozygous DNA deletions, was used to reproduce the homozygous CCR5Δ32 mutation in CD4+ U87 cells...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115407/suppression-of-microrna-130b-inhibits-glioma-cell-proliferation-and-invasion-and-induces-apoptosis-by-pten-akt-signaling
#15
Jian-Jun Gu, Kai-Chun Fan, Jian-He Zhang, Hong-Jie Chen, Shou-Sen Wang
Glioblastoma is the most common malignant brain tumor in adults and is characterized by extensive proliferation and the diffused invasion of tumor cells. Due to the intricate signaling pathways involved in glioma progression, more effective targeted therapies and prognostic biomarkers in clinical practice are required. The suppression of proto-oncogene function or recovery of tumor suppressor gene function remains one of the primary approaches in gene therapy. The close association between the abnormal expression or mutation of microRNA (miRNA) and the tumorigenesis, progression and staging in glioma have been demonstrated previously...
November 2, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29113824/comparative-analysis-of-different-cell-systems-for-zika-virus-zikv-propagation-and-evaluation-of-anti-zikv-compounds-in-vitro
#16
Ilaria Vicenti, Adele Boccuto, Alessia Giannini, Filippo Dragoni, Francesco Saladini, Maurizio Zazzi
A strong correlation between Zika virus (ZIKV) infection and severe neurological disease in newborns and occasionally adults has emerged in the Brazilian outbreak. Efficient human cell-based assays are required to test candidate inhibitors of ZIKV replication. The aim of this work was to investigate ZIKV propagation and quantification in different cell lines. The human (U87, A549, Huh7), mosquito (C6/36) and monkey (VERO E6) cell lines tested were all permissive to ZIKV infection. When assessed by plaque forming units (PFU) in three different target cell lines, the maximal production of ZIKV was achieved in Huh7 at day 3 post-infection (6...
November 4, 2017: Virus Research
https://www.readbyqxmd.com/read/29113309/macrophage-migration-inhibitory-factor-promotes-vasculogenic-mimicry-formation-induced-by-hypoxia-via-cxcr4-akt-emt-pathway-in-human-glioblastoma-cells
#17
Xing Guo, Shugang Xu, Xiao Gao, Jian Wang, Hao Xue, Zihang Chen, Jinsen Zhang, Xiaofan Guo, Mingyu Qian, Wei Qiu, Gang Li
Macrophage migration inhibitory factor (MIF) is over-expressed and secreted in various cancer cells in particular in response to hypoxia. Recent studies have shown that, under hypoxic conditions, glioblastoma (GBM) cells display the ability to drive blood-perfused vasculogenic mimicry (VM). The aim of this study was to investigate the underlying mechanism of MIF in the regulation of hypoxia-induced VM in GBM cells. By analyzing clinical specimens, we observed the co-localization of MIF, C-X-C motif chemokine receptor 4 (CXCR4) and VM in hypoxic regions of gliomas...
October 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/29106517/tpd52l2-impacts-proliferation-invasiveness-and-apoptosis-of-glioblastoma-cells-via-modulation-of-wnt-%C3%AE-catenin-snail-signaling
#18
Qiang Zhou, Jun-Jie Li, Hai Wang, Hong Li, Bing-Xi Lei, Lei Chen, Wei Xiang, Ya-Wei Liu, Annie Huang, Song-Tao Qi, Yun-Tao Lu
Intratumoral heterogeneity greatly hinders efficiency of target therapy in glioblastoma. To decipher the underlying mechanisms of heterogeneity, patient-derived adult glioblastoma (GBM) cells were separately isolated from margins of T1 gadolinium enhancing tumor lesions (PNCs) and T1 gadolinium enhancing core lesions (ECs). Single clone culture was conducted in ECs and U87MG cell line to screen clones with distinct biological phenotypes. Single cell clones with diverse phenotypes were simultaneously separated from ECs and U87 cell line...
November 2, 2017: Carcinogenesis
https://www.readbyqxmd.com/read/29083237/a-new-protein-extract-inhibitor-from-hypobranchial-purple-gland-of-hexaplex-trunculus-a-mediterranean-mollusk-impairs-the-motility-of-human-glioblastoma-u87-and-the-hela-cell-line-of-cervical-carcinoma-cells
#19
Hazem Ben Mabrouk, Sayari Nejia, Morjen Maram, Marrakchi Naziha, Chabchoub-Ellouze Soufia
The aim of this study is to evaluate the effect of hypobranchial gland protein extracts (HGPEs) of Hexaplex trunculus on the viability, cell adhesion, and migration of human U87 glioblastoma cells and the HeLa cell line obtained from epithelial cervical carcinoma cells. Analysis of the HGPE on polyacrylamide gel (12%) shows a variety of proteins whose molecular weights vary between 12 and 1OO kDa. Chromatographic analysis shows 16 peaks obtained at various retention times. Cytotoxic effect was observed after 24 hours of incubation at the concentrations 20, 40, and 60 μg/ml in a dose-dependent manner...
October 30, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/29081036/microrna-378-enhances-radiation-response-in-ectopic-and-orthotopic-implantation-models-of-glioblastoma
#20
Wende Li, Yujiao Liu, Weining Yang, Xiaoxing Han, Sen Li, Hao Liu, Leo E Gerweck, Dai Fukumura, Jay S Loeffler, Burton B Yang, Rakesh K Jain, Peigen Huang
Glioblastoma multiforme (GBM) is the most common and highly malignant primary brain tumor, which is virtually incurable due to its therapeutic resistance to radiation and chemotherapy. To develop novel therapeutic approaches for treatment of GBM, we examined the role of miR-378 on tumor growth, angiogenesis, and radiation response in ectopic and orthotopic U87 glioblastoma models. Cell and tumor growth rates, in vitro and in vivo radiation sensitivities, and tumor vascular density were evaluated in U87-GFP and U87-miR-378 tumor lines...
October 28, 2017: Journal of Neuro-oncology
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