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https://www.readbyqxmd.com/read/28631420/expression-of-pten-long-mediated-by-crispr-cas9-can-repress-u87-cell-proliferation
#1
Na Fang, Tingxuan Gu, Yahui Wang, Shuzhen Wang, Fengling Wang, Yang An, Wenqiang Wei, Weijuan Zhang, Xiangqian Guo, Adil J Nazarali, Shaoping Ji
PTEN is a tumour suppressor that is frequently mutated in a variety of cancers. Hence, PTEN has significant potential as a therapeutic molecule. PTEN-long is an alternative translation variant, with an additional 173 amino acids added to the N-terminal of the canonical PTEN when CUG of the mRNA is utilized as the start codon. PTEN-long is secreted into serum and can re-enter cells throughout the body. One of the major barriers for gene therapy is to efficiently and specifically deliver DNA or RNA material to target cells...
June 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28629521/erythropoietin-promotes-glioblastoma-via-mir-451-suppression
#2
Begum Alural, Zeynep O Ayyildiz, Kemal U Tufekci, Sermin Genc, Kursad Genc
Erythropoietin (EPO) is an erythropoiesis stimulating growth factor and hormone. EPO has been widely used in the treatment of chronic renal failure, cancer, and chemotherapy-related anemia for three decades. However, many clinical trials showed that EPO treatment may be associated with tumorigenesis and cancer progression. EPO is able to cross blood-brain barriers, and this may lead to an increased possibility of central nervous system tumors such as glioblastoma. Indeed, EPO promotes glioblastoma growth and invasion in animal studies...
2017: Vitamins and Hormones
https://www.readbyqxmd.com/read/28628119/differential-expression-of-mir16-in-glioblastoma-and-glioblastoma-stem-cells-their-correlation-with-proliferation-differentiation-metastasis-and-prognosis
#3
R Tian, J Wang, H Yan, J Wu, Q Xu, X Zhan, Z Gui, M Ding, J He
The function of miR16 in multiforme glioblastoma multiforme (GBM) and its stem cells (GSCs) remains elusive. To this end, we investigated the patterns of miR16 expression in these cells and their correlation with malignant behaviors and clinical outcomes. The levels of miR16 and its targeted genes in tumor tissue of GBM and GBM SGH44, U87, U251 cells as well as their stem cell counterparts were measured by qRT-PCR or western blot or immunohistochemistry. Luciferase reporter assay was used to confirm the binding of miR16 to 3'-UTR of its target genes...
June 19, 2017: Oncogene
https://www.readbyqxmd.com/read/28627659/effects-of-saikosaponin-d-on-apoptosis-in-human-u87-glioblastoma-cells
#4
Yan Li, Tongjian Cai, Wei Zhang, Wenhe Zhu, Shijie Lv
Saikosaponin D (SSd) is a type of Saponin derivative, which is a component extracted from Bupleurum falactum. SSd has been reported to exert anticancer activities. However, the effects of SSd on gliomas have not been elucidated. The aim of the present study was to investigate the pharmacological functions and potential molecular mechanisms of SSd in human U87 glioblastoma cells. The cells were treated with SSd at various concentrations for 48 h, the cell viability was assessed with 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium assay, and the activation of Akt, extracellular signal‑regulated kinases (ERK), c‑Jun N‑terminal kinases (JNK) and caspase‑3 was assessed by western blotting...
June 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28619752/the-met-axl-fgfr-inhibitor-s49076-impairs-aurora-b-activity-and-improves-the-antitumor-efficacy-of-radiotherapy
#5
Céline Clémenson, Cyrus Chargari, Winchygn Liu, Michele Mondini, Charles Ferté, Mike F Burbridge, Valérie Cattan, Anne Jacquet-Bescond, Eric Deutsch
Several therapeutic agents targeting HGF/MET signaling are under clinical development as single agents or in combination, notably with anti-EGFR therapies in non-small cell lung cancer (NSCLC). However, despite increasing data supporting a link between MET, irradiation and cancer progression, no data regarding the combination of MET-targeting agents and radiotherapy are available from the clinic. S49076 is an oral ATP-competitive inhibitor of MET, AXL and FGFR1-3 receptors that is currently in phase I/II clinical trials in combination with gefinitib in NSCLC patients whose tumors show resistance to EGFR inhibitors...
June 15, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28615958/the-critical-role-of-egf-%C3%AE-catenin-signaling-in-the-epithelial-mesenchymal-transition-in-human-glioblastoma
#6
Xingqiang Wang, Shanshi Wang, Xiaolong Li, Shigang Jin, Feng Xiong, Xin Wang
To date, β-catenin has been reported to be implicated in mediating the epithelial-mesenchymal transition (EMT) in a variety of human cancers, which can be triggered by EGF. However, the mechanisms underlying EGF-β-catenin pathway-induced EMT of glioblastoma multiforme (GBM) have not been reported previously. In the present study, immunohistochemistry, reverse transcription polymerase chain reaction, and Western blot were applied to investigate the effect of EGF-β-catenin pathway on EMT of GBM. Here, we identified that β-catenin mRNA and protein levels were up-regulated in GBM tissues and four kinds of glioblastoma cell lines, including T98G, A172, U87, and U251 cells, compared with normal brain tissue and astrocytes...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28612256/low-dose-of-doxorubicin-potentiates-the-effect-of-temozolomide-in-glioblastoma-cells
#7
Emilly Schlee Villodre, Franciele Cristina Kipper, Andrew Oliveira Silva, Guido Lenz, Patrícia Luciana da Costa Lopez
Glioblastoma (GBM) is an aggressive brain tumor with temozolomide (TMZ)-based chemotherapy as the main therapeutic strategy. Doxorubicin (DOX) is not used in gliomas due to its low bioavailability in the brain; however, new delivery strategies and low doses may be effective in the long term, especially as part of a drug cocktail. Our aim was to evaluate the chronic effects of low doses of DOX and TMZ in GBM. Human U87-ATCC cells and a primary GBM culture were chronically treated with TMZ (5 μM) and DOX (1 and 10 nM) alone or combined...
June 13, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28608961/high-affinity-click-rgd-peptidomimetics-as-radiolabeled-probes-for-imaging-%C3%AE-v%C3%AE-3-integrin
#8
Monica Piras, Andrea Testa, Ian N Fleming, Sergio Dall'Angelo, Alexandra Andriu, Sergio Menta, Mattia Mori, Gavin D Brown, Duncan Forster, Kaye J Williams, Matteo Zanda
Non-peptidic RGD-mimic ligands were designed and synthesized by click chemistry between an arginine-azide mimic and an aspartic acid-alkyne mimic. Some of these molecules combine excellent in vitro properties (high αvβ3 affinity, selectivity, drug-like logD, high metabolic stability) with a variety of radiolabeling options (e.g. tritium and [18F]fluorine, plus compatibility with radio-iodination), not requiring the use of chelators or prosthetic groups. The binding mode of the resulting triazole RGD-mimics to αvβ3 or αIIbβ3 receptors was investigated by molecular modeling simulations...
June 13, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28607604/preferential-association-of-lissencephaly-1-gene-expression-with-cd133-glioblastoma-cells
#9
Felix Mircea Brehar, Anca Violeta Gafencu, Violeta Georgeta Trusca, Elena Valeria Fuior, Dorel Arsene, Mirela Amaireh, Andrei Giovani, Mircea Radu Gorgan
Lissencephaly-1 (Lis1) protein is a dynein-binding protein involved in neural stem cell division, morphogenesis and motility. To determine whether Lis1 is a key factor in glioblastoma, we evaluated its expression and function in CD133+ glioblastoma cells. Global, Lis1 gene expression is similar in glioblastoma and normal samples. Interestingly, immunohistochemistry data indicate increased Lis1 expression colocalized with CD133 in a subset of glioma cells, including the tumor cells with perivascular localization...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28607028/chemoenzymatic-synthesis-nanotization-and-anti-aspergillus-activity-of-optically-enriched-fluconazole-analogues
#10
Shashwat Malhotra, Seema Singh, Neha Rana, Shilpi Tomar, Priyanka Bhatnagar, Mohit Gupta, Suraj K Singh, Brajendra K Singh, Anil K Chhillar, Ashok K Prasad, Christophe Len, Pradeep Kumar, Kailash C Gupta, Anjani J Varma, Ramesh C Kuhad, Gainda L Sharma, Virinder S Parmar, Nigel G J Richards
Despite recent advances in diagnostic and therapeutic advances in antifungal research, aspergillosis still remains a leading cause of morbidity and mortality. One strategy to address this problem is to enhance the activity spectrum of known antifungals, and we now report the first successful application of Candida antarctica lipase (CAL) for the preparation of optically enriched fluconazole analogs. Anti-Aspergillus activity was observed for an optically enriched derivative, (-)-S-2-(2' ,4' -difluorophenyl)-1-hexyl-amino-3-(1‴,2‴,4‴) triazol-1‴-yl-propan-2-ol, which exhibits MIC values of 15...
June 12, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28602979/inhibition-of-cyp4a-by-a-novel-flavonoid-fla-16-prolongs-survival-and-normalizes-tumor-vasculature-in-glioma
#11
Chenlong Wang, Ying Li, Honglei Chen, Jie Zhang, Jing Zhang, Tian Qin, Chenfan Duan, Xuewei Chen, Yanzhuo Liu, Xiaoyang Zhou, Jing Yang
Glioblastomas rapidly become refractory to anti-VEGF therapies. We previously showed that cytochrome P450 (CYP) 4A-derived 20-hydroxyeicosatetraenoic acid (20-HETE) promotes angiogenesis. Here, we tested whether a novel flavonoid (FLA-16) prolongs survival and normalizes tumor vasculature in glioma through CYP4A inhibition. FLA-16 improved survival, reduced tumor burden, and normalized vasculature, accompanied with the decreased secretion of 20-HETE, VEGF and TGF-β in tumor-associated macrophages (TAMs) and endothelial progenitor cells (EPCs) in C6 and U87 gliomas...
June 7, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28599959/photodynamic-process-induced-by-chloro-aluminum-phthalocyanine-nanoemulsion-in-glioblastoma
#12
Andrielle Castilho-Fernandes, Tácila G Lopes, Fernando L Primo, Marcelo R Pinto, Antonio C Tedesco
BACKGROUND: Glioblastoma multiforme (GBM) is a tumor characterized by rapid cell proliferation and migration. GBM constitutes the most aggressive and deadly type of brain tumor and is classified into several subtypes that show high resistance to conventional therapies. There are currently no curative treatments for malignant glioma despite the numerous advances in surgical techniques, radiotherapy, and chemotherapy. Therefore, alternative approaches are required to improve GBM treatment...
June 6, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28599130/negligible-interaction-of-ru-phen-3-2-with-human-serum-albumin-makes-it-promising-for-a-reliable-invivo-assessment-of-the-tissue-oxygenation
#13
Dominik Belej, Zuzana Jurasekova, Michal Nemergut, Georges Wagnieres, Daniel Jancura, Veronika Huntosova
The interaction between a ruthenium - based water soluble oxygen probe ([Ru(Phen)3](2+), phen - phenanthroline) and human serum albumin (HSA) was investigated with the aim of describing the influence of HSA on the [Ru(Phen)3](2+) luminescence properties. Nowadays, several oxygen sensitive luminescent probes are used to determine the oxygen level in different compartments of living organisms. However, they can interact, depending on their hydrophilic/hydrophobic characters, with various serum proteins, and/or lipids, during their utilization for invivo oxygen measurement...
May 31, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28595721/study-of-antioxidant-effects-on-malignant-glioma-cells-by-constructing-a-tumor-microvascular-structure-on-microchip
#14
Hanyang Liu, Mingsha Jie, Ziyi He, Hai-Fang Li, Jin-Ming Lin
In this work, a three-dimensional tumor-microvascular structure was simulated on a microfluidic chip for study of antioxidants effects on malignant glioma cells in vitro. The 3D hydrogel containing lumen was constructed to co-culture endothelial cells and glioma cells to mimic tumor microvascular environment. Macroporous gelatin transglutaminase (TG) hydrogel was prepared with biological and mechanical properties suitable for cells culture and nutrient refresh. To reform a vessel structure, U87 cells were dispersed in the TG-gelatin hydrogel and HUVEC cells were seeded in the lumen of hydrogel...
July 25, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28591734/application-of-luteolin-nanomicelles-anti-glioma-effect-with-improvement-in-vitro-and-in-vivo
#15
Songping Zheng, Yongzhong Cheng, Yan Teng, Xiaoxiao Liu, Ting Yu, Yi Wang, Jiagang Liu, Yuzhu Hu, Cong Wu, Xiang Wang, Yanhui Liu, Chao You, Xiang Gao, Yuquan Wei
Glioblastoma multiforme (GBM) is one of the most common and malignant tumor. Luteolin, a polyphenolic compound, has been proposed to have anti-tumor activity against various cancers. However, the greatest obstacle in the administration of luteolin is its hydrophobicity as well as the low oral bioavailability. In this study, we formulated luteolin-loaded MPEG-PCL (Luteolin/MPEG-PCL) micelles aiming to improve its solubility in aqueous solution and investigate the anti-tumor effect on glioma in vitro and in vivo...
May 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28591726/multifunctional-targeted-liposomal-drug-delivery-for-efficient-glioblastoma-treatment
#16
Zakia Belhadj, Changyou Zhan, Man Ying, Xiaoli Wei, Cao Xie, Zhiqiang Yan, Weiyue Lu
Glioblastoma multiforme (GBM) has been considered to be the most malignant brain tumors. Due to the existence of various barriers including the blood-brain barrier (BBB) and blood-brain tumor barrier (BBTB) greatly hinder the accumulation and deep penetration of chemotherapeutics, the treatment of glioma remains to be the most challenging task in clinic. In order to circumvent these hurdles, we developed a multifunctional liposomal glioma-targeted drug delivery system (c(RGDyK)/pHA-LS) modified with cyclic RGD (c(RGDyK)) and p-hydroxybenzoic acid (pHA) in which c(RGDyK) could target integrin αvβ3 overexpressed on the BBTB and glioma cells and pHA could target dopamine receptors on the BBB...
May 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28583844/nociceptin-orphanin-fq-antagonizes-lipopolysaccharide-stimulated-proliferation-migration-and-inflammatory-signaling-in-human-glioblastoma-u87-cells
#17
Andrea Bedini, Monica Baiula, Gabriele Vincelli, Francesco Formaggio, Sara Lombardi, Marco Caprini, Santi Spampinato
Glioblastoma is among the most aggressive brain tumors and has an exceedingly poor prognosis. Recently, the importance of the tumor microenvironment in glioblastoma cell growth and progression has been emphasized. Toll-like receptor 4 (TLR4) recognizes bacterial lipopolysaccharide (LPS) and endogenous ligands originating from dying cells or the extracellular matrix involved in host defense and in inflammation. G-protein coupled receptors (GPCRs) have gained interest in anti-tumor drug discovery due to the role that they directly or indirectly play by transactivating other receptors, causing cell migration and proliferation...
June 2, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28579776/aptamer-conjugated-pegylated-quantum-dots-targeting-epidermal-growth-factor-receptor-variant-iii-for-fluorescence-imaging-of-glioma
#18
Jiaze Tang, Ning Huang, Xiang Zhang, Tao Zhou, Ying Tan, Jiangli Pi, Li Pi, Si Cheng, Huzhi Zheng, Yuan Cheng
The extent of resection is a significant prognostic factor in glioma patients. However, the maximum safe resection level is difficult to determine due to the inherent infiltrative character of tumors. Recently, fluorescence-guided surgery has emerged as a new technique that allows safe resection of glioma. In this study, we constructed a new kind of quantum dot (QD)-labeled aptamer (QD-Apt) nanoprobe by conjugating aptamer 32 (A32) to the QDs surface, which can specially bind to the tumors. A32 is a single-stranded DNA capable of binding to the epidermal growth factor receptor variant III (EGFRvIII) specially distributed on the surface of glioma cells...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28578643/glioblastoma-targeted-gene-therapy-based-on-pegfp-p53-loaded-superparamagnetic-iron-oxide-nanoparticles
#19
Touba Eslaminejad, Seyed Noureddin Nematollahi-Mahani, Mehdi Ansari
BACKGROUND: Bloodâ€"brain barrier (BBB) separates the neural tissue from circulating blood because of its high selectivity. This study focused on the in vitro application of magnetic nanoparticles to deliver Tp53 as a gene of interest to glioblastoma (U87) cells across a simulated BBB model that comprised KB cells. MATERIAL AND METHOD: After magnetic and non-magnetic nanoparticles were internalized by KB cells, their location in these cells was examined by transmission electron microscopy...
June 4, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28576721/novel-hybrids-of-natural-%C3%AE-elemene-bearing-isopropanolamine-moieties-synthesis-enhanced-anticancer-profile-and-improved-aqueous-solubility
#20
Jichao Chen, Tianyu Wang, Shengtao Xu, Aijun Lin, Hequan Yao, Weijia Xie, Zheying Zhu, Jinyi Xu
A series of novel β-elemene isopropanolamine derivatives were synthesized and evaluated for their antitumor activity. The results indicated that all of the compounds showed stronger antiproliferative activities than β-elemene as well as improved aqueous solubility. In particular dimer 6q showed the strongest cytotoxicity against four tumor cell lines (SGC-7901, HeLa, U87 and A549) with IC50 values ranging from 4.37 to 10.20μM. Moreover, combination of 6q with cisplatin exhibited a synergistic effect on these cell lines with IC50 values ranging from 1...
May 31, 2017: Fitoterapia
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