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Ming Tsuey Chew, Andrew Nisbet, Masao Suzuki, Naruhiro Matsufuji, Takeshi Murakami, Bleddyn Jones, David A Bradley
Glioblastoma (GBM), a Grade IV brain tumour, is a well-known radioresistant cancer. To investigate one of the causes of radioresistance, we studied the capacity for potential lethal damage repair (PLDR) of three altered strains of GBM: T98G, U87 and LN18, irradiated with various ions and various levels of linear energy transfer (LET). The GBM cells were exposed to 12C and 28Si ion beams with LETs of 55, 100 and 200 keV/μm, and with X-ray beams of 1.7 keV/μm. Mono-energetic 12C ions and 28Si ions were generated by the Heavy Ion Medical Accelerator at the National Institute of Radiological Science, Chiba, Japan...
November 17, 2018: Journal of Radiation Research
Hong Ngoc Thuy Pham, Jennette A Sakoff, Quan Van Vuong, Michael C Bowyer, Christopher J Scarlett
This data article indicates the in vitro cytotoxicity of kaempferol and gallic acid across different cancer cell lines including A2780 (ovarian), H460 (lung), A431 (skin), MIA PaCa-2 (pancreas), Du145 (prostate), HT29 (colon), MCF-7 (breast), BE2-C (neuroblastoma), SJ-G2, U87 and SMA (glioblastoma). The dataset showed that the inhibitory activity of kaempferol was comparatively stronger than gallic acid. Thereby, kaempferol is offered as a potent anticancer agent for further investigation and beneficial as a dietary supplement...
December 2018: Data in Brief
Mostafa A L Abdel-Salam, Juliana Carvalho-Tavares, Kamila Sousa Gomes, Andrea Teixeira-Carvalho, Gregory T Kitten, Johanna Nyffeler, Felipe F Dias, Pablo V Mendes Dos Reis, Adriano M C Pimenta, Marcel Leist, Maria Elena de Lima, Elaine Maria de Souza-Fagundes
Antimicrobial peptides present a broad spectrum of therapeutic applications, including their use as anticancer peptides. These peptides have as target microbial, normal, and cancerous cells. The oncological properties of these peptides may occur by membranolytic mechanisms or non-membranolytics. In this work, we demonstrate for the first time the cytotoxic effects of the cationic alpha-helical antimicrobial peptide LyeTx I-b on glioblastoma lineage U87-MG. The anticancer property of this peptide was associated with a membranolytic mechanism...
November 17, 2018: Amino Acids
Jingxuan Ni, Mengqi Guo, Yucheng Cao, Lei Lei, Kangli Liu, Binghua Wang, Fangfang Lu, Rong Zhai, Xiangwei Gao, Chunhong Yan, Hongbo Wang, Yi Bi
A series of novel fusidic acid (FA) derivatives were synthesized and screened for their in vitro cytotoxicity against the Hela, U87, KBV and MKN45 cancer cell lines. Selected FA derivatives with anti-tumor activity were firstly identified including compound 4, which exhibited good anti-proliferative activity with IC50 values in the range of 1.26-3.57 μM. Further research revealed that compound 4 induced Hela cells to undergo apoptosis by increasing the ratio of the cells in the Sub-G0 /G1 phase via decreasing the neo-synthesized proteins in a dose-dependent manner from 1 to 10 μM...
November 3, 2018: European Journal of Medicinal Chemistry
Ningbo Xu, Boyang Liu, Changlin Lian, Desislava Met Doycheva, Zhao Fu, Yanting Liu, Jian Zhou, Zhenyan He, Zhao Yang, Qiongzhen Huang, Huijun Zeng, Hongbo Guo
Temozolomide (TMZ) and radiation therapy combination for glioblastoma (GB) patients has been considered as the most effective therapy after surgical procedure. However, the overall clinical prognosis remains unsatisfactory due to intrinsic or developing resistance to TMZ. Recently, increasing evidence suggested that long noncoding RNAs (lncRNAs) play a critical role in various biological processes of tumors, and have been implicated in resistance to various drugs. However, the role of lncRNAs in TMZ resistance is poorly understood...
November 15, 2018: Cell Death & Disease
Teresa Lobo-Jarne, Eva Nývltová, Rafael Pérez-Pérez, Alba Timón-Gómez, Thibaut Molinié, Austin Choi, Arnaud Mourier, Flavia Fontanesi, Cristina Ugalde, Antoni Barrientos
The mitochondrial respiratory chain is organized in a dynamic set of supercomplexes (SCs). The COX7A2L protein is essential for mammalian SC III2 +IV assembly. However, its function in respirasome (SCs I+III2 +IVn ) biogenesis remains controversial. To unambiguously determine the COX7A2L role, we generated COX7A2L-knockout (COX7A2L-KO) HEK293T and U87 cells. COX7A2L-KO cells lack SC III2 +IV but have enhanced complex III steady-state levels, activity, and assembly rate, normal de novo complex IV biogenesis, and delayed respirasome formation...
November 13, 2018: Cell Reports
Faisal Alowaidi, Saeed M Hashimi, Maria Nguyen, Mallika Meshram, Naif Alqurashi, Brenton L Cavanagh, Bernadette Bellette, Saso Ivanovski, Adrian Meedenyia, Stephen A Wood
Cripto-1 has been implicated in a number of human cancers. Although there is high potential for a role of Cripto-1 in glioblastoma multiforme (GBM) pathogenesis and progression, few studies have tried to define its role in GBM. These studies were limited in that Cripto-1 expression was not studied in detail in relation to markers of cancer initiation and progression. Therefore, these correlative studies allowed limited interpretation of Criptos-1's effect on the various aspects of GBM development using the U87 GBM cell line...
November 13, 2018: Journal of Cellular Biochemistry
Chao Yang, Longshuang He, Gaofei Chen, Zeqian Ning, Zhibai Xia
Chloride (Cl- ), a primary anion in the extracellular fluid, plays an important role in a variety of physiological and pathological processes, such as cell apoptosis and proliferation. However, the information about Cl- in cancer cell apoptosis and chemoresistance is poorly understood. In the present study, we found that temozolomide (TMZ) treatment led to a decrease in intracellular concentration of Cl- ([Cl- ]i ) in both U87 and TMZ-resistant U87/R glioma cells. The decrease in [Cl- ]i was more noticeable in U87 cells than in U87/R cells...
November 13, 2018: Human Cell
Janel Kydd, Rahul Jadia, Prakash Rai
Polymer-based nanoparticles (NPs) are useful vehicles in treating glioblastoma because of their favorable characteristics such as small size and ability to cross the blood⁻brain barrier, as well as reduced immunogenicity and side effects. The use of a photosensitizer drug such as Verteporfin (BPD), in combination with a pan-vascular endothelial growth factor receptor (VEGFR) tyrosine kinase inhibitor (TKI), Cediranib (CED), encapsulated in NPs will provide the medical field with new research on the possible ways to treat glioblastoma...
November 10, 2018: Pharmaceutics
Chao Qu, Jingyun Ma, Yejun Zhang, Chao Han, Liang Huang, Liming Shen, Hongyan Li, Xiaobo Wang, Jing Liu, Wei Zou
Glioblastoma (GBM) is a highly infiltrative and malignant primary brain tumor. Despite aggressive therapy, patients with GBM have a dismal prognosis with median survival of about one year. Tamoxifen (TAM), a selective estrogen receptor modulator (SERM), has been used to treat GBM for many years. ER-α36 is a novel variant of estrogen receptor-alpha66 (ER-α66) and can mediate cell proliferation through estrogen or anti-estrogen signaling in different cancer cells. Previously, we found that ER-α36 was highly expressed in GBM and was involved in the tamoxifen-sensitivity of glioblastoma cells...
November 11, 2018: Cancer Science
Qi Zhang, Zhihao Han, Ji Tao, Menglu Zhao, Wancun Zhang, Ping Li, Liping Tang, Yueqing Gu
Glypican-3 (GPC3) is a key biomarker for early diagnosis of human hepatocellular carcinoma (HCC) due to its overexpression in most HCC tumor tissues. Recently, peptides with high affinity to GPC3 have attracted more attention because of their high biocompatibility, non-immunogenicity, fast clearing and easy modification. Herein, we have designed an innovative GPC3 targeting peptide (sequence: DYEMHLWWGTEL, denoted as IPA) by using structure-based virtual simulation. The higher binding abilities of IPA over the reported peptide (YP) were displayed on different cell lines, showing a positive correlation with GPC3 expressions, which were further verified by the GPC3 protein binding assay...
November 12, 2018: Biomaterials Science
A V Dadeko, L Lilge, P Kaspler, T D Murav'eva, A M Starodubtcev, V M Kiselev, V V Zarubaev, G V Ponomarev
Photophysical and in vitro photocytotoxicity studies were performed for the photosensitizer Dimegine, a disodium salt 2.4-di(alpha-methoxyethyl)-deuteroporphyrin-IX with very low systemic toxicity. The singlet oxygen and luminescence quantum yield were ΦΔ  = 0,65 ± 0,06, and Φƒ =0,11 ± 0,01 respectively, and were independent of the excitation wavelength. The photobleaching coefficients for Dimegine dissolved in phosphate buffer (pH 7.4), and DMEM medium at concentration 2 μM/l and in phosphate buffer (pH 7...
November 6, 2018: Photodiagnosis and Photodynamic Therapy
Jie Wang, Miao Li, Xiaolin Cui, Dongyue Lv, Lingling Jin, Muhammad Khan, Tonghui Ma
Background: Sesquiterpene lactones are plant-derived, natural, bioactive molecules often used against inflammatory diseases in traditional Chinese medicines. Recently, sesquiterpene lactones have been reported to exhibit potent anticancer activity. In the present study, we have investigated the anticancer activity of Brevilin A, a sesquiterpene lactone component of Centipeda minima , against U87 glioblastoma cells. Materials and methods: The cell proliferation was determined by MTT assay...
2018: OncoTargets and Therapy
Swati Paliwal, Rituparna Chaudhuri, Anurag Agrawal, Sujata Mohanty
BACKGROUND: Recent studies have demonstrated mesenchymal stem cells (MSCs) as effective mitochondrial donors with therapeutic success in multiple experimental models of human disease. MSCs obtained from different tissue sources such as bone marrow (BM), adipose (AD), dental pulp (DP), and Wharton's jelly (WJ) are routinely used in clinical trials with no known study of their mitochondrial donor capacity. Here, we show for the first time that MSCs derived from different tissue sources have different mitochondrial donor properties and that this is correlated with their intrinsic respiratory states...
November 8, 2018: Stem Cell Research & Therapy
Martina Da Ros, Anna Lisa Iorio, Veronica De Gregorio, Ornella Fantappiè, Giacomo Laffi, Maurizio de Martino, Claudio Pisano, Lorenzo Genitori, Iacopo Sardi
Glioblastoma Multiforme (GBM) is still an incurable disease. The front-line Temozolomide (TMZ)-based therapy suffers from poor efficacy, underlining the need of new therapies. Preclinically, Aldoxorubicin (Aldox), a novel prodrug of Doxorubicin (Dox), has been successfully tested against GBM, encouraging the study of its association with other agents. For the first time, we evaluated the effectiveness of Aldox combined to TMZ in preclinical models of GBM. Our in vitro results demonstrated that the anti-glioma effect of Aldox was more marked than TMZ and their combination increased the killing effect of the anthracycline in TMZ-resistant GBM cells...
October 9, 2018: Oncotarget
Fei Zeng, Hong Chen, Zhaohui Zhang, Tao Yao, Guan Wang, Qingxing Zeng, Shenhan Duan, Yanqiang Zhan
To investigate the effects of hypoxia on the features of cancer stem cells in the glioma cancer U87 cell line and underlying mechanism, stem cell markers and features in U87 were studied under the hypoxic and normoxic culture conditions by reverse transcription-quantitative polymerase chain reaction, western blot analysis, MTT, a colony formation test and flow cytometry. Compared to the normoxic group, the cluster of differentiation 133+ phenotype, clone formation rate and cell vitality were significantly elevated in U87 cells cultured in a hypoxic microenvironment...
November 2018: Oncology Letters
Xiaodong Ding, Changqing Ding, Fei Wang, Wenshuai Deng, Mingming Yu, Qinghai Meng, Peng Sun
The present study aimed to observe the effect of the Notch1 signaling inhibitor γ-secretase inhibitor II (GSI II) on the growth and differentiation of tumor cells. The tumor cell line U87 was grown in serum-free media, and cell growth was evaluated using immunofluorescence. Single-cell wall-adherent growing conditions were prepared, GSI II was added, and the differentiation and growth of single tumor cells was evaluated. Immunofluorescence demonstrated positive results for the expression of Nestin and cluster of differentiation 133...
November 2018: Oncology Letters
Ann-Christin Nickel, Xue-Yan Wan, Dino-Vitali Saban, Yin-Lun Weng, Shu Zhang, Kathy Keyvani, Ulrich Sure, Yuan Zhu
PURPOSE: Glioblastoma (GBM) is one of the most aggressive and incurable primary brain tumors. Identification of novel therapeutic targets is an urgent priority. Programmed cell death 10 (PDCD10), a ubiquitously expressed apoptotic protein, has shown a dual function in different types of cancers and in chemo-resistance. Recently, we reported that PDCD10 was downregulated in human GBM. The aim of this study was to explore the function of PDCD10 in GBM cells. METHODS: PDCD10 was knocked down in three GBM cell lines (U87, T98g and LN229) by lentiviral-mediated shRNA transduction...
November 3, 2018: Journal of Neuro-oncology
Yanwen Xu, Renli Liu, Chuanpeng Liao, Jing Liu, Huafu Zhao, Zongyang Li, Wenlan Liu, Lei Chen, Changpeng Wu, Hui Tan, Zhongping Chen, Ni Xie, Weiping Li
Glioma is the commonest malignant tumor in the central nervous system (CNS), characterized by rapid growth. However, the molecular mechanism underlying the growth remains unclear. Immunity-related GTPase family M protein (IRGM) participates in immune response to pathogen and tumorigenesis. Proliferation and autophagy are two crucial functions contributing to aggressive growth. Therefore, our aims were to probe whether IRGM regulates glioma proliferation and autophagy. In this study, we found that 47 glioma specimens had more IRGM expression than 11 non-cancerous brain tissues with immunohistochemistry...
October 24, 2018: Pathology, Research and Practice
Hongchao Li, Qinghua Liang, Lin Wang
Glioblastoma (GBM) is a common and aggressive brain tumor that is associated with significant increase in glycolysis for energy production. Icaritin is a natural compound and exhibits anticancer activity in GBM. However, the effect of icaritin on glycolysis in GBM cells remains unclear. The aim of the current study was to investigate the effect of icaritin on glycolysis in GBM cells. The human GBM cell lines U87 and T98G were treated with icaritin or the inhibitor of Stat3 (S3I-201) in the presence or absence of recombinant human interleukin (IL)-6...
November 2, 2018: Journal of Cellular Biochemistry
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