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https://www.readbyqxmd.com/read/28237688/the-flashlights-on-a-distinct-role-of-protein-kinase-c-%C3%AE-phosphorylation-of-regulatory-and-catalytic-domain-upon-oxidative-stress-in-glioma-cells
#1
Matus Misuth, Jaroslava Joniova, Denis Horvath, Lenka Dzurova, Zuzana Nichtova, Marta Novotova, Pavol Miskovsky, Katarina Stroffekova, Veronika Huntosova
Glioblastoma multiforme are considered to be aggressive high-grade tumors with poor prognosis for patient survival. Photodynamic therapy is one of the adjuvant therapies which has been used for glioblastoma multiforme during last decade. Hypericin, a photosensitizer, can be employed in this treatment. We have studied the effect of hypericin on PKCδ phosphorylation in U87 MG cells before and after light application. Hypericin increased PKCδ phosphorylation at tyrosine 155 in the regulatory domain and serine 645 in the catalytic domain...
February 22, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28229962/overexpression-of-hsa-mir-939-follows-by-ngfr-down-regulation-and-apoptosis-reduction
#2
Fahimeh Hosseini Aghdaei, Bahram M Soltani, Sadat Dokanehiifard, Seyed Javad Mowla, Masoud Soleimani
Neurotrophin receptors play a crucial role in neuronal survival, differentiation and regeneration. Nerve growth factor receptor (NGFR) or P75(NTR) is a neurotrophin receptor that is involved in many pathological conditions including cancers. Genetic factors that are involved in regulation of neurotrophin receptors are under intense investigation. MiRNAs are novel regulators of signalling pathways that are candidates for regulation of neurotrophin receptors. Computational programs predicted that NGFR gene is a bona fide target for hsa-miR- 939...
March 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/28223165/heterogeneous-intratumoral-distribution-of-gadolinium-nanoparticles-within-u87-human-glioblastoma-xenografts-unveiled-by-micro-pixe-imaging
#3
Asuncion Carmona, Stéphane Roudeau, Baptiste L'Homel, Frédéric Pouzoulet, Sarah Bonnet-Boissinot, Yolanda Prezado, Richard Ortega
Metallic nanoparticles have great potential in cancer radiotherapy as theranostic drugs since, they serve simultaneously as contrast agents for medical imaging and as radio-therapy sensitizers. As with other anticancer drugs, intratumoral diffusion is one of the main limiting factors for therapeutic efficiency. To date, a few reports have investigated the intratumoral distribution of metallic nanoparticles. The aim of this study was to determine the quantitative distribution of gadolinium (Gd) nanoparticles after direct intratumoral injection within U87 human glioblastoma tumors grafted in mice, using micro-PIXE (Particle Induced X-ray Emission) imaging...
February 18, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28222026/hypoxic-regulation-of-the-expression-of-genes-encoded-estrogen-related-proteins-in-u87-glioma-cells-eff-ect-of-ire1-inhibition
#4
Minchenko Do, Riabovol Oo, Ratushna Oo, Minchenko Oh
OBJECTIVE: The aim of the present study was to examine the effect of inhibition of endoplasmic reticulum stress signaling, mediated by IRE1 (inositol requiring enzyme 1), which is a central mediator of the unfolded protein response on the expression of genes encoded estrogen related proteins (NRIP1/RIP140, TRIM16/EBBP, ESRRA/NR3B1, FAM162A/E2IG5, PGRMC2/PMBP, and SLC39A6/LIV-1) and their hypoxic regulation in U87 glioma cells for evaluation of their possible significance in the control of glioma cells proliferation...
January 1, 2017: Endocrine Regulations
https://www.readbyqxmd.com/read/28220178/a-photo-degradable-supramolecular-hydrogel-for-selective-delivery-of-microrna-into-3d-cultured-cells
#5
Zhengquan Zhou, Qikun Yi, Tingting Xia, Wencui Yin, Adnan A Kadi, Jinbo Li, Yan Zhang
Multi-functional supramolecular hydrogels have emerged as smart biomaterials for diverse biomedical applications. Here we report a multi-functional supramolecular hydrogel formed by the conjugate of the bioactive GRGDS peptide with biaryltetrazole that is the substrate of photo-click reaction. The hydrogel was used as a biocompatible matrix to encapsulate live cells for 3D culture. The presence of the RGD epitope in the hydrogelator enhanced the interaction of the nanofiber with integrin over-expressing cells, which resulted in the selective enhancement in the miRNA delivery into the encapsulated U87 cells...
February 21, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28219405/ttbk2-circular-rna-promotes-glioma-malignancy-by-regulating-mir-217-hnf1%C3%AE-derlin-1-pathway
#6
Jian Zheng, Xiaobai Liu, Yixue Xue, Wei Gong, Jun Ma, Zhuo Xi, Zhongyou Que, Yunhui Liu
BACKGROUND: Circular RNAs are a subgroup of non-coding RNAs and generated by a mammalian genome. Herein, the expression and function of circular RNA circ-TTBK2 were investigated in human glioma cells. METHODS: Fluorescence in situ hybridization and quantitative real-time PCR were conducted to profile the cell distribution and expression of circ-TTBK2 and microRNA-217 (miR-217) in glioma tissues and cells. Immunohistochemical and western blot were used to determine the expression of HNF1β and Derlin-1 in glioma tissues and cells...
February 20, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28205096/pharmacological-effects-of-asiatic-acid-in-glioblastoma-cells-under-hypoxia
#7
Flourina Kumar Thakor, Ka-Wai Wan, Philip John Welsby, Gail Welsby
Glioblastoma multiforme is the most common and malignant primary brain tumor in adults. Despite current treatment options including surgery followed by radiation and chemotherapy with temozolomide and cisplatin, the median survival rate remains low (<16 months). Combined with increasing drug resistance and the inability of some compounds to cross the blood-brain barrier, novel compounds are being sought for the treatment of this disease. Here, we aimed to examine the pharmacological effect of Asiatic acid (AA) in glioblastoma under hypoxia...
February 15, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28203118/human-u87-glioblastoma-cells-with-stemness-features-display-enhanced-sensitivity-to-natural-killer-cell-cytotoxicity-through-altered-expression-of-nkg2d-ligand
#8
Se-Jeong Oh, Jung-In Yang, Ok Kim, Eun-Jung Ahn, Woo Dae Kang, Jae-Hyuk Lee, Kyung-Sub Moon, Kyung-Hwa Lee, Duck Cho
BACKGROUND: Glioblastoma (GBM) is one of the most lethal tumors with a poor prognosis. Its inevitable recurrence is frequently explained by the presence of cancer stem cells. We aimed to show that human GBM cells with stemness features are more sensitive to natural killer (NK) cells than GBM cells without stemness characteristics. METHODS: Natural killer cell cytotoxicity was measured using flow cytometry in neurosphere-forming U87 GBM cells cultured with neurobasal media (NBE condition) and compared with that in serum-cultured U87 GBM cells (serum condition)...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28197638/cobalt-chloride-treatment-induces-autophagic-apoptosis-in-human-glioma-cells-via-a-p53-dependent-pathway
#9
Bor-Chin Cheng, Jui-Tai Chen, Shun-Tai Yang, Chung-Ching Chio, Shing-Hwa Liu, Ruei-Ming Chen
Malignant glioma is the most aggressive brain tumor. Hypoxic condition has been explored for killing cancer stem cells or drug-resistant tumor cells. This study investigated the effects of hypoxia on autophagic death and the possible mechanisms. Exposure of human malignant glioma U87-MG cells to cobalt chloride (CoCl2) increased cellular hypoxia-inducible factor-1α levels and concurrently decreased cell viability concentration- and time-dependently. In parallel, treatment with CoCl2 suppressed proliferation of human U87-MG cells...
March 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28193559/dual-functional-mesoporous-silicon-nanoparticles-enhance-the-radiosensitivity-of-vpa-in-glioblastoma
#10
Hailong Zhang, Wei Zhang, Yong Zhou, Yuhua Jiang, Shupeng Li
Radiotherapy is a critical strategy and standard adjuvant approach to glioblastoma treatment. One of the major challenges facing radiotherapy is to minimize radiation damage to normal tissue without compromising therapeutic effects on cancer cells. Various agents and numerous approaches have been developed to improve the therapeutic index of radiotherapy. Among them, radiosensitizers have attracted much attention because they selectively increase susceptibility of cancer cells to radiation and thus enhance biological effectiveness of radiotherapy...
February 10, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28191852/artificial-chemical-reporter-targeting-strategy-using-bioorthogonal-click-reaction-for-improving-active-targeting-efficiency-of-tumor
#11
Hong Yeol Yoon, Min Lee Shin, Man Kyu Shim, Sangmin Lee, Jin Hee Na, Heebeom Koo, Hyukjin Lee, Jong-Ho Kim, Kuen Yong Lee, Kwangmeyung Kim, Ick Chan Kwon
Biological ligands such as aptamer, antibody, glucose and peptide have been widely used to bind specific surface molecules or receptors in tumor cells or subcellular structures to improve tumor-targeting efficiency of nanoparticles. However, this active targeting strategy has limitations for tumor targeting due to inter- and intra-heterogeneity of tumors. In this study, we demonstrated an alternative active targeting strategy using metabolic engineering and bioorthogonal click reaction to improve tumor-targeting efficiency of nanoparticles...
February 13, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28191621/the-effect-of-ascorbic-acid-over-the-etoposide-and-temozolomide-mediated-cytotoxicity-in-glioblastoma-cell-culture-a-molecular-study
#12
Dilek Göktürk, Hasim Kelebek, Seda Ceylan, Derviş Mansuri Yilmaz
AIM: Glioblastoma (GBM) is one of the lethal central nervous system tumors. One of the widely used chemical agents for treatment of glioblastoma is temozolomide. It is an orally administered, DNA alkylating agent. DNA alkylation triggers the death of tumor cells. However, some tumor cells are able to repair this type of DNA damage, thus lower the therapeutic effect of temozolomide. Laboratory and clinical studies indicate that temozolomide\'s anticancer effects might be strengthened when combined with other chemotherapeutic agents, like etoposide, or antioxidant agents, like ascorbic acid...
January 19, 2017: Turkish Neurosurgery
https://www.readbyqxmd.com/read/28188727/apoptin-gene-delivery-by-the-functionalized-polyamidoamine-pamam-dendrimer-derivatives-induces-cell-death-of-u87-mg-glioblastoma-cells
#13
Yoonhee Bae, Hyang-Shuk Rhim, Seulgi Lee, Kyung Soo Ko, Jin Han, Joon Sig Choi
Malignant glioma is the most common and aggressive form of primary brain tumor in adults. In this study, we describe the efficacy of nonviral gene delivery carriers, histidine and arginine or histidine and lysine grafted PAMAM dendrimers (PAMAM-H-R and PAMAM-H-K), in delivering a therapeutic, a tumor-selective killer gene, apoptin, using human glioma cells (U87-MG) and newborn human dermal fibroblast (NB-HDF) cells. We analyzed transfection efficiency using luciferase (Luc) and a pDNA encoding for enhanced fluorescent protein (EGFP), and assessed cell viability in both cells...
February 7, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28187755/long-noncoding-rna-papillary-thyroid-carcinoma-susceptibility-candidate-3-ptcsc3-inhibits-proliferation-and-invasion-of-glioma-cells-by-suppressing-the-wnt-%C3%AE-catenin-signaling-pathway
#14
Shujun Xia, Ri Ji, Weiwei Zhan
BACKGROUND: The dysregulation of long noncoding RNAs (lncRNAs) has been identified in a variety of cancers. An increasing number of studies have found the critical role of lncRNAs in the regulation of cellular processes, such as proliferation, invasion and differentiation. Long noncoding RNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) is a novel lncRNA that was primarily detected in papillary thyroid carcinoma. However, the biological function and molecular mechanism of lncRNA PTCSC3 in glioma are still unknown...
February 10, 2017: BMC Neurology
https://www.readbyqxmd.com/read/28185834/eva1a-inhibits-gbm-cell-proliferation-by-inducing-autophagy-and-apoptosis
#15
Xue Shen, Shifeng Kan, Zhen Liu, Guang Lu, Xiaoyan Zhang, Yingyu Chen, Yun Bai
Eva-1 homolog A (EVA1A) is a novel lysosome and endoplasmic reticulum-associated protein involved in autophagy and apoptosis. In this study, we constructed a recombinant adenovirus 5-EVA1A vector (Ad5-EVA1A) to overexpress EVA1A in glioblastoma (GBM) cell lines and evaluated its anti-tumor activities in vitro and in vivo. We found that overexpression of EVA1A in three GBM cell lines (U251, U87 and SHG44) resulted in a suppression of tumor cell growth via activation of autophagy and induction of cell apoptosis in a dose- and time-dependent manner...
March 1, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28184932/microrna-520c-inhibits-glioma-cell-migration-and-invasion-by-the-suppression-of-transforming-growth-factor-%C3%AE-receptor-type%C3%A2-2
#16
Shengli Hu, Huibin Chen, Yuqiang Zhang, Chaojia Wang, Kaijun Liu, Hui Wang, Jie Luo
Dysregulation of microRNAs (miRNAs) is actively involved in the development and progression of glioma. miR-520c was previously found to inhibit glioblastoma cell migration. However, the clinical significance of miR-520c and its biological function in glioma remain largely unknown. In the present study, we found that miR-520c expression in glioma tissues was significantly decreased compared to adjacent non‑cancerous tissues. The low level of miR-520c was prominently correlated with advanced World Health Organization (WHO) grade and decreased overall survival of glioma patients...
March 2017: Oncology Reports
https://www.readbyqxmd.com/read/28168356/identifying-survival-associated-modules-from-the-dysregulated-triplet-network-in-glioblastoma-multiforme
#17
Jia-Bin Wang, Feng-Hua Liu, Jian-Hang Chen, Hai-Tao Ge, Lu-Yan Mu, Hong-Bo Bao, Zhi-Guo Lin
BACKGROUND: Long noncoding RNAs (lncRNAs) can act as competitive endogenous RNAs (ceRNAs) to compete with mRNAs for binding miroRNAs (miRNAs). The dysregulated triplets, composed by mRNAs, lncRNAs, and miRNAs, contributed to the development and progression of diseases, such as cancer. However, the roles played by triplet biomarkers are not fully understand in glioblastoma multiforme (GBM) patient survival. OBJECTIVES: Here, we constructed a differential triplet interaction network (TriNet) between GBM and normal tissues and identified GBM survival related triplets...
February 6, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28168193/proliferative-and-invasive-effects-of-progesterone-induced-blocking-factor-in-human-glioblastoma-cells
#18
Araceli Gutiérrez-Rodríguez, Valeria Hansberg-Pastor, Ignacio Camacho-Arroyo
Progesterone-induced blocking factor (PIBF) is a progesterone (P4) regulated protein expressed in different types of high proliferative cells including astrocytomas, the most frequent and aggressive brain tumors. It has been shown that PIBF increases the number of human astrocytoma cells. In this work, we evaluated PIBF regulation by P4 and the effects of PIBF on proliferation, migration, and invasion of U87 and U251 cells, both derived from human glioblastomas. PIBF mRNA expression was upregulated by P4 (10 nM) from 12 to 24 h...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28153956/influence-of-bevacizumab-on-blood-brain-barrier-permeability-and-o-2-18f-fluoroethyl-l-tyrosine-uptake-in-rat-gliomas
#19
Carina Stegmayr, Dennis Oliveira, Nicole Niemietz, Antje Willuweit, Philipp Lohmann, Norbert Galldiks, N Jon Shah, Johannes Ermert, Karl-Josef Langen
PURPOSE: Restoration of the blood-brain barrier (BBB) following antiangiogenic therapy of gliomas using Bevacizumab may result in a decrease of contrast enhancement in magnet resonance imaging (MRI) despite tumor progression. This so called pseudoresponse is difficult to differentiate from true tumor response with conventional MRI. First patient studies have indicated that positron emission tomography (PET) using O-(2-(18)F-fluoroethyl)-L-tyrosine ((18)F-FET) may be helpful to solve this diagnostic problem...
February 2, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28153732/zika-virus-induces-inflammasome-activation-in-the-glial-cell-line-u87-mg
#20
Paola Maura Tricarico, Ilaria Caracciolo, Sergio Crovella, Pierlanfranco D'Agaro
In the last years, neurological complications related to Zika virus (ZIKV) infection have emerged as an important threat to public health worldwide. ZIKV infection has been associated to neurological disorders such as congenital microcephaly in newborns and Guillain-Barré syndrome, myelopathy and encephalitis in adults. ZIKV is characterized by neurotropism and neurovirulence. Several studies have identified microglial nodules, gliosis, neuronal and glial cells degeneration and necrosis in the brain of ZIKV infected infants, suggesting that ZIKV could play a role in these neurological disorders through neuroinflammation and microglial activation...
January 30, 2017: Biochemical and Biophysical Research Communications
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