Read by QxMD icon Read

monocyte neutrophil

Roberto Anaya-Prado, Manuel Fernando Barba-Anaya, Jorge Manuel Sánchez-González, Michelle Marie Anaya-Fernández, Ana Bertha Zavalza-Gómez, Francisco Gil-Villarreal, Alejandro Bravo-Cuellar, Andrea Del Carmen Grijalva-Solis
Studies have shown that triggering receptor expressed on myeloid cells-1 (TREM-1) is the mediator and activator of neutrophils and monocytes after stimulation with lipopolysaccharide (LPS), heat-inactivated Gram (-) bacteria, Gram (+) bacteria or fungi. Different studies have measured the expression of TREM-1 in patients with bacterial infections and critical states. The purpose of this study was to evaluate the expression of TREM-1 in circulating maternal leukocytes in premature rupture of the membranes (PRM)...
October 18, 2016: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
Charles Nicaise, Laura Weischelbaum, Liliane Schandene, Valérie Gangji, Florence Dehavay, Joanna Bouchat, Benoît Balau, Thomas Vogl, Muhammad S Soyfoo
OBJECTIVES: To determine the role of S100A8/A9 in the pathogenesis of primary Sjögren's syndrome (pSS). METHODS: The serum levels of S100A8/A9 were determined in pSS patients and healthy controls by ELISA. The expression of S100A8/A9 in salivary glands was assessed by immunohistochemistry. The phenotype of S100A8+ and S100A9+ cells was identified using double immunofluorescence. The effects of S100A8/A9 on cytokine production by peripheral blood mononuclear cells (PBMCs) from pSS patients were determined in vitro by flow cytometry...
October 7, 2016: Clinical and Experimental Rheumatology
Kaitlyn Sadtler, Brian W Allen, Kenneth Estrellas, Franck Housseau, Drew M Pardoll, Jennifer H Elisseeff
The immune system mediates tissue growth and homeostasis and is the first responder to injury or biomaterial implantation. Recently, it has been appreciated that immune cells play a critical role in wound healing and tissue repair and should thus be considered as potentially beneficial particularly in the context of scaffolds for regenerative medicine. Here, we present a flow cytometric analysis of cellular recruitment to tissue-derived extracellular matrix scaffolds where we quantitatively describe the infiltration and polarization of several immune subtypes including macrophages, dendritic cells, neutrophils, monocytes, T cells, and B cells...
October 13, 2016: Tissue Engineering. Part A
Silvia Lee, Markus Durstberger, Beate Eichelberger, Christoph W Kopp, Renate Koppensteiner, Simon Panzer, Thomas Gremmel
BACKGROUND: The beneficial effects of ß-blockers on the long-term prognosis of patients with cardiovascular disease may in part be attributable to decreased platelet activation. In this prospective cohort study, we sought to investigate the impact of concomitant ß-blocker therapy on sensitive markers of platelet activation and aggregation. MATERIALS AND METHODS: Monocyte- (MPA) and neutrophil-platelet aggregate (NPA) formation in vivo and in response to the platelet agonist adenosine diphosphate (ADP) were determined by flow cytometry in 258 patients undergoing angioplasty and stenting...
October 13, 2016: European Journal of Clinical Investigation
Rachael D Wright, Patricia R Souza, Magdalena B Flak, Prasheetha Thedchanamoorthy, Lucy V Norling, Dianne Cooper
Galectin-3 has been associated with a plethora of proinflammatory functions because of its ability, among others, to promote neutrophil activation and because of the reduction in neutrophil recruitment in models of infection in Gal-3-null mice. Conversely, it has also been linked to resolution of inflammation through its actions as an opsonin and its ability to promote efferocytosis of apoptotic neutrophils. Using a self-resolving model of peritonitis, we have addressed the modulation and role of Gal-3 in acute inflammation...
October 12, 2016: Journal of Leukocyte Biology
Ahmet Goktug Ertem, Cagri Yayla, Burak Acar, Ozgur Kırbas, Sefa Unal, Melahat Uzel Sener, Mehmet Kadri Akboga, Tolga Han Efe, Serkan Sıvrı, Fatih Sen, Serkan Gokaslan, Serkan Topaloglu
INTRODUCTION: The relationship between inflammation and mortality after acute pulmonary embolism (APE) has previously been investigated with different variables (platelet/lymphocyte ratio etc.) Objectives: We investigated the predictive value of lymphocye to monocyte ratio (LMR) for mortality in first 30 days after APE. METHODS: The study population included 294 APE patients of which 230 patients survivors, 64 patients non-survivors. RESULTS: Lymphocye to monocyte ratio was significantly lower in non-survivors after APE (p<0...
October 11, 2016: Clinical Respiratory Journal
Donald Metcalf
This review is restricted to neutrophilic granulocytes (granulocytes), monocytes (macrophages), and eosinophils, with only passing reference to cells that are also usually included in the "myeloid" category-megakaryocytes, mast cells, and erythroid cells. Although some dendritic cells are of myeloid origin, they are discussed elsewhere. The validity of the information to be described depends on two assumptions: (a) that in vitro data are applicable to events in vivo and (b) that mouse data reflect events in man...
August 2016: Microbiology Spectrum
O Skor, A Fuchs-Baumgartinger, A Tichy, M Kleiter, I Schwendenwein
Leukocyte ratios correlate with outcome in several human cancers. Little is known about their prognostic significance in mast cell tumour (MCT). The aim of the study was to evaluate the prognostic significance of pretreatment leukocyte concentrations and their ratios in dogs with MCT for survival. Medical records of 92 dogs with MCT were retrospectively reviewed. Tumour diagnosis was made by tumour biopsy or fine-needle aspirate. Only dogs without prior treatment were included. Eosinophil, lymphocyte, monocyte and neutrophil concentration were obtained by ADVIA 2120™ (Siemens Healthcare, Vienna, Austria)...
October 9, 2016: Veterinary and Comparative Oncology
Anja J Gerrits, Andrew L Frelinger, Alan D Michelson
In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. This leukocyte-platelet aggregate formation is initiated primarily through platelet surface expression of P-selectin (CD62P), following activation-dependent degranulation of α-granules, binding to its constitutively expressed counter-receptor, P-selectin glycoprotein ligand 1 (PSGL-1), on leukocytes. Monocyte-platelet aggregates are a more sensitive marker of platelet activation than platelet surface P-selectin...
October 10, 2016: Current Protocols in Cytometry
Cheryl Sarah Philipose, T Umashankar
INTRODUCTION: Malaria is a mosquito borne disease which is a major public health problem and a leading cause of morbidity and mortality worldwide. Various haematological parameters have been studied to help predict malaria, such as alteration in the leucocyte count, platelet counts and erythrocyte counts. The neutrophil lymphocyte count ratio (NLCR) was found to have a good predictive value in systemic inflammation, particularly in critical care setting. AIMS AND OBJECTIVES: The present study aims to study the various haematological parameters and acertain the predictive value of NLCR and MLR in the detection of malaria...
July 2016: Tropical Parasitology
Liang Ming, Ruo Yu Peng, Lei Zhang, Chun Li Zhang, Pin Lv, Zhong Quan Wang, Jing Cui, Hui Jun Ren
As we all know, invasion of host intestinal epithelium is very important for T. spiralis to complete successfully their life cycle. However, the mechanisms that the intestinal infective larvae (IIL) invade and migrate in the intestinal epithailial cells (IECs) remain unclear until now. The related researches have been hindered since a readily operable in vitro normal model. In our earlier study, an in vitro normal IEC invasion model was established for the first time, and the abilities of the normal IECs to initiate mucosal inflammatory responses to invasion by the IIL in vitro were evaluated in this study...
October 4, 2016: Experimental Parasitology
Sylwia Mańka, Ewa Majewska
Literature data indicate a significant immunoregulatory role of melatonin. Melatonin exerts an effect directly affecting leucocytes bearing specific melatonin receptors or indirectly by means of melatonin regulating other hormones, opioids or cytokines. Despite numerous experiments, the influence of the hormone on the immune system is still controversial. Melatonin affects the immune response acting as both an activator and an inhibitor of the inflammatory process. The hormone acts as an "immunological buffer" activating impaired immunity in immunosuppression, chronic stress or old age as well as suppressing overreaction of the immune system...
October 4, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
Natascha Luther, Fatemeh Shahneh, Melanie Brähler, Franziska Krebs, Sven Jäckel, Saravanan Subramaniam, Christian Stanger, Tanja Schönfelder, Bettina Kleis-Fischer, Christoph Reinhardt, Hans C Probst, Philip Wenzel, Katrin Schäfer, Christian Becker
RATIONALE: Immune cells play an important role during the generation and resolution of thrombosis. T cells are powerful regulators of immune and non-immune cell function, however their role in sterile inflammation in venous thrombosis has not been systematically examined. OBJECTIVE: This study investigated the recruitment, activation and inflammatory activity of T cells in deep vein thrombosis (DVT) and its consequences for venous thrombus resolution. METHODS AND RESULTS: CD4(+) and CD8(+) T cells infiltrate the thrombus and vein wall rapidly upon DVT induction and remain in the tissue throughout thrombus resolution...
October 5, 2016: Circulation Research
Aurélie Gabrion, Isabelle Hmitou, Despina Moshous, Bénédicte Neven, Alain Lefèvre-Utile, Jean-Sébastien Diana, Félipe Suarez, Capucine Picard, Stéphane Blanche, Alain Fischer, Marina Cavazzana, Fabien Touzot
BACKGROUND: Chronic granulomatous disease (CGD) is primary immunodeficiency caused by defective production of reactive oxygen species in phagocytic cells that results in life-threatening infections and severe inflammatory manifestations. The treatment of inflammatory manifestations remains challenging, as it can be associated with an increased risk of infections. Previous studies have shown that CGD phagocytes display a defect in autophagy and a reactive oxygen species-independent activation of the inflammasome...
October 1, 2016: Journal of Allergy and Clinical Immunology
Camila A Pazzini, Luciano J Pereira, Ana P Peconick, Leandro S Marques, Saul M Paiva
The aim of this study was to assess periodontal status and blood parameters in orthodontic patients with nickel allergy one month after removal of brackets. Ninety-six randomly selected patients were initially evaluated. Allergy to nickel was diagnosed using a patch test. After determining the prevalence of subjects allergic to nickel, two groups were formed: 16 allergic (experimental) and 16 non-allergic (control) patients. Their periodontal status was determined regularly by a single, blinded, duly calibrated examiner using the Löe Index (GI) and their blood was tested (complete blood test, including nickel and IgE levels) after nine months of orthodontic treatment and again one month after removing the orthodontic appliances...
April 2016: Acta Odontológica Latinoamericana: AOL
Silvia Cellone Trevelin, Célio Xavier Dos Santos, Raphael Gomes Ferreira, Larissa de Sá Lima, Rangel Leal Silva, Cristoforo Scavone, Rui Curi, José Carlos Alves-Filho, Thiago Mattar Cunha, Pérsio Roxo-Júnior, Maria-Célia Cervi, Francisco Rafael Martins Laurindo, John Stephen Hothersall, Andrew M Cobb, Min Zhang, Aleksandar Ivetic, Ajay M Shah, Lucia Rossetti Lopes, Fernando Queiroz Cunha
The reactive-oxygen-species-(ROS)-generating-enzyme Nox2 is essential for leukocyte anti-microbial activity. However its role in cellular redox homeostasis and, consequently, in modulating intracellular signaling pathways remains unclear. Herein, we show Nox2 activation favors thioredoxin-1 (TRX-1)/p40phox interaction, which leads to exclusion of TRX-1 from the nucleus. In contrast, the genetic deficiency of Nox2 or its pharmacological inhibition with apocynin (APO) results in reductive stress after lipopolysaccharide-(LPS)-cell stimulation, which causes nuclear accumulation of TRX-1 and enhanced transcription of inflammatory mediators through nuclear-factor-(NF)-κB...
October 4, 2016: Scientific Reports
Timothy M Williams, Andrea F Wise, Daniel S Layton, Sharon D Ricardo
BACKGROUND AND AIM: Kidney ischemia/reperfusion (IR) injury is characterised by tubular epithelial cell (TEC) death and an inflammatory response involving cytokine production and immune cell infiltration. In various kidney diseases, increased macrophage numbers correlate with injury severity and poor prognosis. However, macrophage plasticity enables a diverse range of functions, including wound healing, making them a key target for novel therapies. This study aimed to comprehensively characterise the changes in myeloid and epithelial cells and the production of cytokines throughout the experimental IR model of acute kidney injury to aid in the identification of targets to promote and enhance kidney regeneration and repair...
October 1, 2016: Nephrology
Maria E Stålhammar, Lena Douhan Håkansson, Anders Jonzon, Richard Sindelar
BACKGROUND: A prerequisite for an effective innate immunity is the migrative ability of neutrophils to respond to inflammatory and infectious agents such as the intermediate interleukin (IL)-8 and the end-target formyl-methionyl-leucyl-phenylalanine (fMLP) chemoattractants. The aim was to study the chemotactic capacity of neutrophils from newborn infants and adults in response to IL-8 and the bacterial peptide fMLP. METHODS: In the under-agarose cell migration assay, isolated leukocytes from healthy adults and from cord blood of healthy term newborn infants were studied with dose responses towards IL-8 and fMLP...
October 3, 2016: Upsala Journal of Medical Sciences
Erin R Green, Stacie Clark, Gregory T Crimmins, Matthias Mack, Carol A Kumamoto, Joan Mecsas
All three pathogenic Yersinia species share a conserved virulence plasmid that encodes a Type 3 Secretion System (T3SS) and its associated effector proteins. During mammalian infection, these effectors are injected into innate immune cells, where they block many bactericidal functions, including the production of reactive oxygen species (ROS). However, Y. pseudotuberculosis (Yptb) lacking the T3SS retains the ability to colonize host organs, demonstrating that chromosome-encoded factors are sufficient for growth within mammalian tissue sites...
September 2016: PLoS Pathogens
Qi Zhang, Cuiping Zhang, Fangzhi Chang, Kun Liang, Xiaoyan Yin, Xiaoyu Li, Kun Zhao, Qinghui Niu, Zibin Tian
Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a chronically intestinal autoimmune disease, the pathological mechanisms of which are not very clear. Wild type p-53 induced phosphatase 1 (Wip1), a serine/threonine protein phosphatase, has been reported to negatively regulate the inflammation in sepsis. However, the role of Wip1 in IBD is not very clear. Therefore, colonic tissues and peripheral blood from patients with IBD and healthy controls were collected to analyzed mRNA and protein expression of Wip1 using the method of qPCR and immunohistochemistry...
July 25, 2016: Cellular Immunology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"