Marike Palmer, Jonathan K Covington, En-Min Zhou, Scott C Thomas, Neeli Habib, Cale O Seymour, Dengxun Lai, Juliet Johnston, Ameena Hashimi, Jian-Yu Jiao, Alise R Muok, Lan Liu, Wen-Dong Xian, Xiao-Yang Zhi, Meng-Meng Li, Leslie P Silva, Benjamin P Bowen, Katherine Louie, Ariane Briegel, Jennifer Pett-Ridge, Peter K Weber, Elitza I Tocheva, Tanja Woyke, Trent R Northen, Xavier Mayali, Wen-Jun Li, Brian P Hedlund
Although the phylum Chloroflexota is ubiquitous, its biology and evolution are poorly understood due to limited cultivability. Here, we isolated two motile, thermophilic bacteria from hot spring sediments belonging to the genus Tepidiforma and class Dehalococcoidia within the phylum Chloroflexota. A combination of cryo-electron tomography, exometabolomics, and cultivation experiments using stable isotopes of carbon revealed three unusual traits: flagellar motility, a peptidoglycan-containing cell envelope, and heterotrophic activity on aromatics and plant-associated compounds...
April 11, 2023: ISME Journal