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MicroRNA transplantation

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https://www.readbyqxmd.com/read/27917123/mir-150-suppresses-the-proliferation-and-tumorigenicity-of-leukemia-stem-cells-by-targeting-the-nanog-signaling-pathway
#1
Dan-Dan Xu, Peng-Jun Zhou, Ying Wang, Yi Zhang, Rong Zhang, Li Zhang, Su-Hong Chen, Wu-Yu Fu, Bi-Bo Ruan, Hai-Peng Xu, Chao-Zhi Hu, Lu Tian, Jin-Hong Qin, Sheng Wang, Xiao Wang, Qiu-Ying Liu, Zhe Ren, Xue-Kui Gu, Yao-He Li, Zhong Liu, Yi-Fei Wang
Proliferation, a key feature of cancer cells, accounts for the majority of cancer-related diseases resulting in mortality. MicroRNAs (miRNAs) plays important post-transcriptional modulation roles by acting on multiple signaling pathways, but the underlying mechanism in proliferation and tumorigenicity is unclear. Here, we identified the role of miR-150 in proliferation and tumorigenicity in leukemia stem cells (LSCs; CD34+CD38- cells). miR-150 expression was significantly down-regulated in LSCs from leukemia cell lines and clinical samples...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27913008/microrna-98-plays-a-critical-role-in-experimental-myocarditis
#2
Xiao Chen, Shuo Dong, Ningning Zhang, Liang Chen, Mao-Gang Li, Ping-Chang Yang, Jiangping Song
BACKGROUND AND AIMS: Myocarditis is inflammation in the heart; its pathogenesis is to be further investigated. Activities of micro RNAs (miR) are associated with immune inflammation. This study tests a hypothesis that miR-98 is involved in the development of myocarditis. METHODS: BALB/c mice were immunized with cardiac α-myosin heavy chain peptides (MyHC-α) to induce myocarditis. The effects of miR-98 on regulation of interleukin (IL)-10 were assessed by real time RT-PCR...
November 23, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27893611/absence-of-mir-182-augments-cardiac-allograft-survival
#3
Liang Wei, Vandana Kaul, Xiumei Qu, Xiaoxing Xiong, Audrey H Lau, Naoharu Iwai, Olivia M Martinez, Sheri M Krams
BACKGROUND: MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate the posttranscriptional expression of target genes and are important regulators in immune responses. Previous studies demonstrated that the miRNA, miR-182 was significantly increased during allograft rejection. Further, the transcription factor Forkhead box (FOX) protein 1, (FOXO1) was shown to be a target of miR-182. The aim of this study is to further examine the role of miR-182 in alloimmune responses. METHODS: Transplantation of BALB/c cardiac allografts was performed in C57BL/6, miR-182, B6...
November 23, 2016: Transplantation
https://www.readbyqxmd.com/read/27874233/induction-of-hepatocyte-like-cells-from-human-umbilical-cord-derived-mesenchymal-stem-cells-by-defined-micrornas
#4
Xia Zhou, Lina Cui, Xinmin Zhou, Qiong Yang, Lu Wang, Guanya Guo, Yu Hou, Weile Cai, Zheyi Han, Yongquan Shi, Ying Han
Generating functional hepatocyte-like cells (HLCs) from mesenchymal stem cells (MSCs) is of great urgency for bio-artificial liver support system (BALSS). Previously, we obtained HLCs from human umbilical cord-derived MSCs by overexpressing seven microRNAs (HLC-7) and characterized their liver functions in vitro and in vivo. Here, we aimed to screen out the optimal miRNA candidates for hepatic differentiation. We sequentially removed individual miRNAs from the pool and examined the effect of transfection with remainder using RT-PCR, periodic acid-Schiff (PAS) staining and low-density lipoprotein (LDL) uptake assays and by assessing their function in liver injury models...
November 22, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27870736/lidocaine-administration-controls-micrornas-alterations-observed-after-lung-ischemia-reperfusion-injury
#5
Lisa Rancan, Carlos Simón, Emmeline Marchal-Duval, Javier Casanova, Sergio Damian Paredes, Alberto Calvo, Cruz García, David Rincón, Agustín Turrero, Ignacio Garutti, Elena Vara
BACKGROUND: Ischemia-reperfusion injury (IRI) is associated with morbidity and mortality. MicroRNAs (miRNAs) have emerged as regulators of IRI, and they are involved in the pathogenesis of organ rejection. Lidocaine has proven anti-inflammatory activity in several tissues but its modulation of miRNAs has not been investigated. This work aims to investigate the involvement of miRNAs in lung IRI in a lung auto-transplantation model and to investigate the effect of lidocaine. METHODS: Three groups (sham, control, and Lidocaine), each comprising 6 pigs, underwent a lung autotransplantation...
December 2016: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/27866993/micrornas-223-3p-and-93-5p-in-patients-with-chronic-kidney-disease-before-and-after-renal-transplantation
#6
M Ulbing, A H Kirsch, B Leber, S Lemesch, J Münzker, N Schweighofer, D Hofer, O Trummer, A R Rosenkranz, H Müller, K Eller, V Stadlbauer, B Obermayer-Pietsch
Chronic kidney disease (CKD) is associated with a multifactorial dysregulation of bone and vascular calcification and closely linked to increased cardiovascular mortality and concomitant bone disease. We aimed to investigate specific microRNA (miRNA) signatures in CKD patients to find indicators for vascular calcification and/or bone mineralization changes during CKD and after kidney transplantation (KT). A miRNA array was used to investigate serum miRNA profiles in CKD patients, then selected miRNAs were quantified in a validation cohort comprising 73 patients in CKD stages 3 to 5, 67 CKD patients after KT, and 36 healthy controls...
November 17, 2016: Bone
https://www.readbyqxmd.com/read/27863127/study-on-genetic-stability-in-human-urothelial-cells-in-vitro
#7
Clara Ibel Chamorro, Said Zeiai, Gisela Reinfeldt Engberg, David Brodin, Johanna Lundin, Agneta Nordenskjöld, Magdalena Fossum
Quality control studies addressing gene expression changes and genetic stability are of vital importance in regenerative medicine. In order to rule out that in vitro expansion gives rise to gene expression changes that could favor oncogenic events, we applied a total human gene expression chip (Affymetrix®) and bioinformatics analysis using the Ingenuity web-based application in combination with an analysis of chromosomal copy number variations using array CGH. We found that urothelial cells presented a general repression of genes required for cell cycle progression and an upregulated of growth-inhibitory genes, as well as a decrease in DNA replication after long-term culture...
November 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27855713/microrna-29-facilitates-transplantation-of-bone-marrow-derived-mesenchymal-stem-cells-to-alleviate-pelvic-floor-dysfunction-by-repressing-elastin
#8
Minfei Jin, Yuelin Wu, Jun Wang, Weiping Ye, Lei Wang, Peipei Yin, Wei Liu, Chenhao Pan, Xiaolin Hua
BACKGROUND: Pelvic floor dysfunction (PFD) is a condition affecting many women worldwide, with symptoms including stress urinary incontinence (SUI) and pelvic organ prolapse (POP). We have previously demonstrated stable elastin-expressing bone marrow-derived mesenchymal stem cells (BMSCs) attenuated PFD in rats, and aim to further study the effect of microRNA-29a-3p regulation on elastin expression and efficacy of BMSC transplantation therapy. METHODS: We inhibited endogenous microRNA-29a-3p in BMSCs and investigated its effect on elastin expression by RT-PCR and Western blot...
November 17, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27844280/stem-cells-as-a-promising-tool-for-the-restoration-of-brain-neurovascular-unit-and-angiogenic-orientation
#9
REVIEW
Mohammad Hossein Geranmayeh, Alireza Nourazarian, Çığır Biray Avci, Reza Rahbarghazi, Mehdi Farhoudi
Establishing systemic therapeutics for neuro-inflammatory diseases is a main objective of regenerative medicine, certainly focusing on restoration of BBB dysfunction and neurovascular reconstitution. In this context, the regenerative capacity and therapeutic effects of different stem cells have been recently found to bring hopeful outcomes through trans-differentiation, paracrine interactions, and molecular cross-talk of transplanted stem cells with resident cells of blood-brain barrier. Here, we highlight some of the most recent scientific breakthroughs and discoveries by different authorities...
November 14, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27829839/therapeutic-potential-of-stem-cells-strategy-for-cardiovascular-diseases
#10
REVIEW
Chang Youn Lee, Ran Kim, Onju Ham, Jihyun Lee, Pilseog Kim, Seokyeon Lee, Sekyung Oh, Hojin Lee, Minyoung Lee, Jongmin Kim, Woochul Chang
Despite development of medicine, cardiovascular diseases (CVDs) are still the leading cause of mortality and morbidity worldwide. Over the past 10 years, various stem cells have been utilized in therapeutic strategies for the treatment of CVDs. CVDs are characterized by a broad range of pathological reactions including inflammation, necrosis, hyperplasia, and hypertrophy. However, the causes of CVDs are still unclear. While there is a limit to the currently available target-dependent treatments, the therapeutic potential of stem cells is very attractive for the treatment of CVDs because of their paracrine effects, anti-inflammatory activity, and immunomodulatory capacity...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27826917/mirna-profiling-in-human-induced-pluripotent-stem-cells
#11
Erica Hennessy
Human iPS cells are capable of differentiation towards all three germ layer lineages. As well as being of use for the study of developmental biology, these cells also provide an excellent resource for disease modeling and cell replacement therapies. iPS cells derived from an individual with a disease type of interest can be differentiated towards the cell type afflicted in that particular disease. Such differentiated cell types can be used to test patient-specific drug responses in vitro, for generation of replacement cells for transplantation, or as a starting material for gene editing to correct disease-causing mutations/deletions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27826601/plasma-micrornas-are-potential-biomarkers-of-acute-rejection-after-hindlimb-transplantation-in-rats
#12
Hiroki Oda, Ryosuke Ikeguchi, Hirofumi Yurie, Yukitoshi Kaizawa, Souichi Ohta, Koji Yamamoto, Tomoki Aoyama, Shuichi Matsuda
BACKGROUND: The development of effective immunosuppressive regimens has resulted in many cases of successful hand transplantation. Visual skin inspection and histological evaluation are used to assess the rejection of hand transplants, but these methods are largely subjective. In this study, we aimed to determine the potential of microRNAs (miRNAs) as biomarkers for acute rejection in vascularized composite allotransplants. METHODS: In allograft group, 7 male Brown-Norway rats (RT1(n)) were used as donors and 13 male Lewis rats (RT1(l)) were used as recipients...
November 2016: Transplantation Direct
https://www.readbyqxmd.com/read/27817193/effect-of-microrna-155-on-the-interferon-gamma-signaling-pathway-in-biliary-atresia
#13
Yu-An Hsu, Chih-Hung Lin, Hui-Ju Lin, Chi-Chun Huang, Hsiu-Chu Lin, Ying-Chi Chen, Ching-Yao Chang, Su-Hua Huang, Jane-Ming Lin, Kuan-Rong Lee, Lei Wan
MicroRNAs (miRNAs) are ~22-nucleotide long RNAs that negatively regulate gene expression and inflammatory responses in eukaryotes. The aim of this work was to evaluate the roles of miRNA (miR)-155 on the interferon-γ (IFN-γ)-induced response in biliary atresia (BA), which is the most common form of pediatric chronic liver disease and a leading indication for pediatric liver transplantation. The expression of miR-155 and the suppressor of cytokine signaling 1 (SOCS1) gene in human and mice liver tissues of BA and healthy controls was evaluated...
December 31, 2016: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/27807762/stem-cell-derived-exosomes-autophagy-extracellular-matrix-turnover-and-mirnas-in-cardiac-regeneration-during-stem-cell-therapy
#14
REVIEW
Priyanka Prathipati, Shyam Sundar Nandi, Paras Kumar Mishra
Stem cell therapy (SCT) raises the hope for cardiac regeneration in ischemic hearts. However, underlying molecular mechanisms for repair of dead myocardium by SCT in the ischemic heart is poorly understood. Growing evidences suggest that cardiac matrix stiffness and differential expressions of miRNAs play a crucial role in stem cell survival and differentiation. However, their roles on transplanted stem cells, for myocardial repair of the ischemic heart, remain unclear. Transplanted stem cells may act in an autocrine and/or paracrine manner to regenerate the dead myocardium...
November 2, 2016: Stem Cell Reviews
https://www.readbyqxmd.com/read/27775615/microrna-mediated-down-regulation-of-apoptosis-signal-regulating-kinase-1-ask1-attenuates-the-apoptosis-of-human-mesenchymal-stem-cells-mscs-transplanted-into-infarcted-heart
#15
Chang Youn Lee, Sunhye Shin, Jiyun Lee, Hyang-Hee Seo, Kyu Hee Lim, Hyemin Kim, Jung-Won Choi, Sang Woo Kim, Seahyung Lee, Soyeon Lim, Ki-Chul Hwang
Stem cell therapy using adult stem cells, such as mesenchymal stem cells (MSCs) has produced some promising results in treating the damaged heart. However, the low survival rate of MSCs after transplantation is still one of the crucial factors that limit the therapeutic effect of stem cells. In the damaged heart, oxidative stress due to reactive oxygen species (ROS) production can cause the death of transplanted MSCs. Apoptosis signal-regulating kinase 1 (ASK1) has been implicated in the development of oxidative stress-related pathologic conditions...
October 20, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27775550/microrna-29b-mediates-altered-innate-immune-development-in-acute-leukemia
#16
Bethany L Mundy-Bosse, Steven D Scoville, Li Chen, Kathleen McConnell, Hsiaoyin C Mao, Elshafa H Ahmed, Nicholas Zorko, Sophia Harvey, Jordan Cole, Xiaoli Zhang, Stefan Costinean, Carlo M Croce, Karilyn Larkin, John C Byrd, Sumithira Vasu, William Blum, Jianhua Yu, Aharon G Freud, Michael A Caligiuri
Natural killer (NK) cells can have potent antileukemic activity following haplo-mismatched, T cell-depleted stem cell transplantations for the treatment of acute myeloid leukemia (AML), but they are not successful in eradicating de novo AML. Here, we have used a mouse model of de novo AML to elucidate the mechanisms by which AML evades NK cell surveillance. NK cells in leukemic mice displayed a marked reduction in the cytolytic granules perforin and granzyme B. Further, as AML progressed, we noted the selective loss of an immature subset of NK cells in leukemic mice and in AML patients...
December 1, 2016: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27773452/significant-role-for-microrna-21-affecting-toll-like-receptor-pathway-in-primary-graft-dysfunction-after-human-lung-transplantation
#17
Zhongping Xu, Monal Sharma, Andrew Gelman, Ramsey Hachem, Thalachallour Mohanakumar
BACKGROUND: MicroRNAs (miRNAs) were recently identified as modulators of immune responses after human lung transplantation (LTx). This study was undertaken to assess the contribution of miRNAs to the pathogenesis of primary graft dysfunction (PGD) after LTx. METHODS: Of the 39 recipients, 14 (35.9%) developed Grade 3 PGD (i.e., severe PGD) within the first 72 hours of LTx. The remaining 25 recipients (64.1%) had Grade 2 or less PGD, and served as the control group...
September 12, 2016: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/27747027/developing-mirna-therapeutics-for-cardiac-repair-in-ischemic-heart-disease
#18
Kai Zhu, Dingqian Liu, Hao Lai, Jun Li, Chunsheng Wang
MicroRNAs (miRNAs) families have been found to be powerful regulators in a wide variety of diseases, which enables the possible use of miRNAs in therapeutic strategies for cardiac repair after ischemic heart disease. This review provides some general insights into miRNAs modulation for development of current molecular and cellular therapeutics in cardiac repair, including endogenous regeneration, endogenous repair, stem cells transplantation, and reprogramming. We also review the delivery strategies for miRNAs modulation, and briefly summarize the current bench and clinical efforts that are being made to explore miRNAs as the future therapeutic target...
September 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27746218/recipient-hla-c-haplotypes-and-microrna-148a-b-binding-sites-have-no-impact-on-allogeneic-hematopoietic-cell-transplantation-outcomes
#19
Gretchen A Hoff, Johannes C Fischer, Katharine Hsu, Sarah Cooley, Jeffrey S Miller, Tao Wang, Michael Haagenson, Stephen Spellman, Stephanie J Lee, Markus Uhrberg, Jeffrey M Venstrom, Michael R Verneris
Natural killer cells are important in graft-versus-leukemia responses after hematopoietic cell transplantation (HCT). A variety of surface receptors dictates natural killer cell function, including killer cell immunoglobulin-like receptor recognition of HLA-C. Previous single-center studies show that HLA-C epitopes, designated C1 and C2, were associated with allogeneic HCT outcomes; specifically, recipients homozygous for the C1 epitope (C1/C1) experienced a survival benefit. Additionally, mismatching at HLA-C was beneficial in recipients possessing at least 1 C2 allele, whereas the opposite was true for homozygous C1 (C1/C1) recipients where HLA-C mismatching resulted in worse outcomes...
October 13, 2016: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/27744427/role-of-beclin-1-mediated-autophagy-in-the-survival-of-pediatric-leukemia-cells
#20
Xiaoli Wu, Xuefeng Feng, Xiaoqing Zhao, Futian Ma, Na Liu, Hongming Guo, Chaonan Li, Huan Du, Baoxi Zhang
BACKGROUND/AIMS: Acute and chronic leukemia are severe malignant cancers worldwide, and can occur in pediatric patients. Since bone marrow cell transplantation is seriously limited by the availability of the immune-paired donor sources, the therapy for pediatric leukemia (PL) remains challenging. Autophagy is essential for the regulation of cell survival in the harsh environment. However, the role of autophagy in the survival of PL cells under the oxidative stress, e.g. chemotherapy, remain ill-defined...
October 17, 2016: Cellular Physiology and Biochemistry
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