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MicroRNA transplantation

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https://www.readbyqxmd.com/read/28733386/mir-99-regulates-normal-and-malignant-hematopoietic-stem-cell-self-renewal
#1
Mona Khalaj, Carolien M Woolthuis, Wenhuo Hu, Benjamin H Durham, S Haihua Chu, Sarah Qamar, Scott A Armstrong, Christopher Y Park
The microRNA-99 (miR-99) family comprises a group of broadly conserved microRNAs that are highly expressed in hematopoietic stem cells (HSCs) and acute myeloid leukemia stem cells (LSCs) compared with their differentiated progeny. Herein, we show that miR-99 regulates self-renewal in both HSCs and LSCs. miR-99 maintains HSC long-term reconstitution activity by inhibiting differentiation and cell cycle entry. Moreover, miR-99 inhibition induced LSC differentiation and depletion in an MLL-AF9-driven mouse model of AML, leading to reduction in leukemia-initiating activity and improved survival in secondary transplants...
July 21, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28723642/suppression-of-microrna-384-enhances-autophagy-of-airway-smooth-muscle-cells-in-asthmatic-mouse
#2
Zhe Cheng, Xi Wang, Lingling Dai, Liuqun Jia, Xiaogang Jing, Ying Liu, Huan Wang, Pengfei Li, Lin An, Meng Liu
Injury to airway smooth muscle (ASM) cells hallmarks the pathological progression of asthma, a chronic inflammatory airway disease. MicroRNAs (miRNAs) play essential roles in the development of asthma as well as airway remodeling. Here we studied the involvement of miRNAs in the regulation of autophagic survival of ASM cells and airway disorder. We analyzed autophagy-associated factors LC3 and Beclin-1 by RT-qPCR and protein blotting in purified airway smooth muscle cells from ovalbumin (OVA)-induced asthmatic mice...
July 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28713918/endothelial-growth-medium-suppresses-apoptosis-of-mesenchymal-stem-cells-in-vitro-via-decrease-of-mir%C3%A2-29a
#3
Qianqian Wu, Tao Fang, Min Chen, Guoxian Qi
The administration of mesenchymal stem cells (MSCs) in cases of cardiac ischemia/reperfusion injury (IRI) has been associated with a significant reduction of myocardial cell death and an effective improvement in cardiac function. However, one major limiting factor in MSCs transplantation therapy is the low survival rate of the transplanted cells. The present study aimed to demonstrate that human amnion‑derived mesenchymal stem cells (hAMSCs) cultured with endothelial growth medium (EGM‑2) exhibited reduced apoptosis when exposed to serum‑free and hypoxic conditions; and that the expression of microRNA (miR)‑29a decreased significantly...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28711328/overexpression-of-microrna-30a-contributes-to-the-development-of-aortic-dissection-by-targeting-lysyl-oxidase
#4
Yang Yu, Enyi Shi, Tianxiang Gu, Rui Tang, Shilun Gao, Yongchao Wang, Hongbo Liu
OBJECTIVE: To explore the role of microRNA (miR)-30a in the development of aortic dissection. METHODS: Human aortic specimens of aortic dissections and aneurysms were harvested. Aortic specimens from donors for heart transplantation served as controls. Rat aortic vascular smooth muscle cells (VSMCs) were transfected with agomiR-30a or antagomiR-30a, and control cells were incubated with empty vectors. Rats were pretreated with agomiR-30a or antagomiR-30a (5 × 10(7) transfection units every 3 days for 4 weeks), and empty vectors were infused to controls...
June 16, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28710827/comparison-of-non-coding-rnas-in-exosomes-and-functional-efficacy-of-human-embryonic-stem-cell-versus-induced-pluripotent-stem-cell-derived-cardiomyocytes
#5
Won Hee Lee, Wenyi Chen, Ning-Yi Shao, Dan Xiao, Xulei Qin, Natalie Baker, Hye Ryeong Michelle Bae, Praveen Shukla, Haodi Wu, Kazuki Kodo, Sang-Ging Ong, Joseph C Wu
BACKGROUND: Both human embryonic stem cell-derived cardiomyocytes (ESC-CMs) and human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) can serve as unlimited cell sources for cardiac regenerative therapy. However, the functional equivalency between human ESC-CMs and iPSC-CMs for cardiac regenerative therapy has not been demonstrated. Here we performed a head-to-head comparison of ESC-CMs and iPSC-CMs in their ability to restore cardiac function in a rat myocardial infarction (MI) model as well as their exosomal secretome...
July 14, 2017: Stem Cells
https://www.readbyqxmd.com/read/28706977/acute-rejection-after-kidney-transplantation-associates-with-circulating-micrornas-and-vascular-injury
#6
Roel Bijkerk, Barend W Florijn, Meriem Khairoun, Jacques M G J Duijs, Gurbey Ocak, Aiko P J de Vries, Alexander F Schaapherder, Marko J K Mallat, Johan W de Fijter, Ton J Rabelink, Anton Jan van Zonneveld, Marlies E J Reinders
BACKGROUND: Acute rejection (AR) of kidney transplants is associated with the loss of endothelial integrity, microvascular rarefaction and, ultimately, graft dysfunction. Circulating angiogenic microRNAs (miRNAs) may serve as markers for microvascular injury. Here, we investigated the short- and long-term effects of AR after kidney transplantation on systemic vascular injury and the associated circulating miRNA profile. METHODS: Systemic vascular injury was determined by measuring capillary tortuosity and density within the oral mucosa as well as by assessing circulating levels of angiopoietin-2/angiopoietin-1 ratio, vascular endothelial growth factor and soluble thrombomodulin...
July 2017: Transplantation Direct
https://www.readbyqxmd.com/read/28704388/the-mir-23a-27a-24-2-microrna-cluster-buffers-transcription-and-signaling-pathways-during-hematopoiesis
#7
Jeffrey L Kurkewich, Justin Hansen, Nathan Klopfenstein, Helen Zhang, Christian Wood, Austin Boucher, Joseph Hickman, David E Muench, H Leighton Grimes, Richard Dahl
MicroRNA cluster mirn23a has previously been shown to promote myeloid development at the expense of lymphoid development in overexpression and knockout mouse models. This polarization is observed early in hematopoietic development, with an increase in common lymphoid progenitors (CLPs) and a decrease in all myeloid progenitor subsets in adult bone marrow. The pool size of multipotential progenitors (MPPs) is unchanged; however, in this report we observe by flow cytometry that polarized subsets of MPPs are changed in the absence of mirn23a...
July 13, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28681918/microrna-137-is-negatively-associated-with-clinical-outcome-and-regulates-tumor-development-through-ezh2-in-cervical-cancer
#8
Huimin Zhang, Ting Yan, Zhijun Liu, Jun Wang, Yu Lu, Donglin Li, Wentong Liang
PURPOSE: We intend to evaluate the expression, clinical relevance and functional role of microRNA-137 (miR-137) in human cervical cancer (CC). METHODS: MiR-137 expressions were assessed by qPCR in CC cell lines and human CC tumors. The correlation between endogenous miR-137 expression and CC Patients' postoperative overall survival was examined statistically. CC cell lines, Ca-Ski and SiHa cells were transduced with lentivirus to ectopically upregulate endogenous miR-137 expressions...
July 6, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28670859/high-expression-of-cpne3-predicts-adverse-prognosis-in-acute-myeloid-leukemia
#9
Lin Fu, Huaping Fu, Jianlin Qiao, Yifan Pang, Keman Xu, Lei Zhou, Qingyun Wu, Zhenyu Li, Xiaoyan Ke, Kailin Xu, Jinlong Shi
CPNE3, a member of a Ca(2+) -dependent phospholipid-binding protein family, was identified as a ligand of ERBB2 and has a more general role in carcinogenesis. Here, we identified the prognostic significance of CPNE3 expression in acute myeloid leukemia (AML) patients based on two datasets. In the first microarray datasets (n=272), high CPNE3 expression (CPNE3(high) ) was associated with adverse overall survival (OS, P < 0.001) and event-free survival (EFS, P < 0.001) comparing to low CPNE3 expression (CPNE3(low) )...
July 3, 2017: Cancer Science
https://www.readbyqxmd.com/read/28670383/mir-155-up-regulated-by-tgf-%C3%AE-promotes-epithelial-mesenchymal-transition-invasion-and-metastasis-of-human-hepatocellular-carcinoma-cells-in-vitro
#10
Da-Peng Li, Juan Fan, Yong-Jun Wu, Yu-Feng Xie, Juan-Min Zha, Xiu-Min Zhou
It has previously been reported that microRNA (miR)-155 is linked to the recurrence and prognosis of hepatocellular carcinoma (HCC) following liver transplantation. However, the role of miR-155 in the invasion and metastasis of HCC cells remains largely unclear. The aim of this study was to investigate the expression of miR-155 in HCC cells and its role in the invasion and migration of HCC cells in vitro. We found that the level of expression of miR-155 in HCC tissues and cells was significantly increased compared with non-tumorous adjacent tissues...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28662151/bone-marrow-mesenchymal-stem-cell-derived-vascular-endothelial-growth-factor-attenuates-cardiac-apoptosis-via-regulation-of-cardiac-mirna-23a-and-mirna-92a-in-a-rat-model-of-myocardial-infarction
#11
Yi-Sun Song, Hyun-Woo Joo, In-Hwa Park, Guang-Yin Shen, Yonggu Lee, Jeong Hun Shin, Hyuck Kim, Kyung-Soo Kim
Bone marrow-mesenchymal stem cell (BM-MSC) therapy improves the recovery of cardiac function after myocardial infarction (MI); however, the underlying molecular mechanisms are not completely understood. Recent studies have shown that microRNAs (miRNAs) modulate the pathophysiology of cardiovascular diseases. Here, we investigated the mechanisms underlying the effects of BM-MSC-derived paracrine factors and cardiac miRNAs on myocardial regeneration after MI. In our study, MI was induced by permanent ligation of the left anterior descending (LAD) coronary artery...
2017: PloS One
https://www.readbyqxmd.com/read/28643818/serum-micrornas-are-altered-in-various-stages-of-chronic-kidney-disease-a-preliminary-study
#12
COMMENT
Benjamin Brigant, Valérie Metzinger-Le Meuth, Ziad A Massy, Nathalie McKay, Sophie Liabeuf, Marion Pelletier, Marion Sallée, Eléonore M'Baya-Moutoula, Pascale Paul, Tilman B Drueke, Stéphane Burtey, Laurent Metzinger
BACKGROUND: MicroRNAs (miRNAs) are innovative and informative blood-based biomarkers involved in numerous pathophysiological processes. In this study and based on our previous experimental data, we investigated miR-126, miR-143, miR-145, miR-155 and miR-223 as potential circulating biomarkers for the diagnosis and prognosis of patients with chronic kidney disease (CKD). The primary objective of this study was to assess the levels of miRNA expression at various stages of CKD. METHODS: RNA was extracted from serum, and RT-qPCR was performed for the five miRNAs and cel-miR-39 (internal control)...
February 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28642684/ferulic-acid-improves-functional-recovery-after-acute-spinal-cord-injury-in-rats-by-inducing-hypoxia-to-inhibit-microrna-590-and-elevate-vascular-endothelial-growth-factor-expressions
#13
Zhenjie Li, Shengyun Wang, Wenfang Li, Hongbin Yuan
Spinal cord injury (SCI) is the leading cause of paralysis, disability and even death in severe cases, and neural stem cells (NSCs) transplant has been employed for repairing SCI. Ferulic acid (FA) is able to promote neurogenesis in various stem cell therapies. We aimed to investigate the effect of FA on NSC transplant therapy, and the underlying mechanism, in improving functional recovery in SCI rat model. A rat model of SCI was established, which then received transplant of NSCs with or without FA pre-treatment...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28642130/mir-155-expression-is-downregulated-in-kidney-transplant-patients-with-human-cytomegalovirus-infection
#14
Massimiliano Bergallo, Stefano Gambarino, Paola Montanari, Valentina Daprà, Marco Rassu, Ilaria Galliano, Paolo Ravanini
MicroRNAs are small, noncoding RNAs that control expression of target genes through inhibiting protein translation or inducing degradation of mRNA transcripts of target genes. According to the latest update, 2578 unique mature miRNAs are currently annotated in the human genome and participate in the regulation of multiple events, such as cellular proliferation or apoptosis. Herpesvirus family comprehends many viruses able to control and modulate host-cell processes permitting the survival by a latency phase after primary infection...
June 19, 2017: Transplant Immunology
https://www.readbyqxmd.com/read/28640790/microrna-155-deficiency-in-kupffer-cells-ameliorates-liver-ischemia-reperfusion-injury-in-mice
#15
Yakun Li, Dongxia Ma, Zhimin Wang, Jun Yang
BACKGROUND: MicroRNA-155 (miR-155) is known to be involved in autoimmune diseases, inflammation, and transplantation. However, its role in a warm hepatic ischemia-reperfusion (IR) model has not been fully elucidated. METHODS: Partial hepatic IR was performed in wild-type and miR-155-deficient mice treated with or without GdCl3, and then the serum transaminase concentration and histology were analyzed. Kupffer cells (KCs) were isolated from the liver after IR, and immunohistochemistry was used to evaluate activation and polarization...
July 2017: Transplantation
https://www.readbyqxmd.com/read/28637783/paradoxical-suppression-of-atherosclerosis-in-the-absence-of-microrna-146a
#16
Henry S Cheng, Rickvinder Besla, Angela Li, Zhiqi Chen, Eric A Shikatani, Maliheh Nazari-Jahantigh, Adel Hammoutène, My-Anh Nguyen, Michele Geoffrion, Lei Cai, Nadiya Khyzha, Tong Li, Sonya A MacParland, Mansoor Husain, Myron I Cybulsky, Chantal M Boulanger, Ryan E Temel, Andreas Schober, Katey J Rayner, Clinton Robbins, Jason E Fish
Rationale: Inflammation is a key contributor to atherosclerosis. MicroRNA-146a (miR-146a) has been identified as a critical brake on pro-inflammatory NF-κB signalling in several cell types, including endothelial cells and bone marrow-derived cells. Importantly, miR-146a expression is elevated in human atherosclerotic plaques, and polymorphisms in the miR-146a pre-cursor have been associated with risk of coronary artery disease. Objective: To define the role of endogenous miR-146a during atherogenesis. Methods and Results: Paradoxically, Low-density lipoprotein receptor (Ldlr)(-/-) mice deficient in miR-146a develop less atherosclerosis, despite having highly elevated levels of circulating pro-inflammatory cytokines...
June 21, 2017: Circulation Research
https://www.readbyqxmd.com/read/28624527/significantly-altered-expression-of-mir-511-3p-and-its-target-akt3-has-negative-prognostic-value-in-human-prostate-cancer
#17
Fan Zhang, Zhongjun Wu
PURPOSE: In this study, we assessed the expression and functions of microRNA-511-3p (miR-511-3p) in human prostate cancer (CaP). METHODS: Gene expressions of miR-511-3p in CaP cells and human CaP tumors were assessed by qPCR. In VCaP and PC3 cells, miR-511-3p was overexpressed by lentivirus. The functions of miR-511-3p upregulation in regulating in vitro cancer proliferation, migration and in vivo cancer growth were assessed by MTT, transwell and transplantation assays, respectively...
June 14, 2017: Biochimie
https://www.readbyqxmd.com/read/28619106/bone-mesenchymal-stem-cells-ameliorate-ischemia-reperfusion-induced-damage-in-renal-epithelial-cells-via-microrna-223
#18
Xiaopeng Yuan, Xiaoping Wang, Chuanbao Chen, Jian Zhou, Ming Han
BACKGROUND: Recent studies have indicated that microRNA-223 (miR-223) plays a role in the tissue-protective effect of mesenchymal stem cells (MSCs). NLR family-pyrin domain containing 3 (NLRP3) was reported to affect a renal ischemia/reperfusion (I/R) injury by exerting a direct effect on the renal tubular epithelium. Therefore, we investigated how miR-223 and NLRP3 might function in kidneys exposed to conditions of ischemia and subsequent reperfusion. METHODS: Hypoxia/reoxygenation (H/R) murine renal tubular epithelial cells (RTECs) were cocultured with either MSCs or hypoxia-pretreated MSCs (htMSCs), after which the RTECs were examined for their viability and evidence of apoptosis...
June 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28602220/inhibition-of-microrna-24-increases-liver-fibrosis-by-enhanced-menin-expression-in-mdr2-mice
#19
Chad Hall, Laurent Ehrlich, Fanyin Meng, Pietro Invernizzi, Francesca Bernuzzi, Terry C Lairmore, Gianfranco Alpini, Shannon Glaser
BACKGROUND: Liver transplantation remains the primary treatment for primary sclerosing cholangitis (PSC). Mdr2(-/-) mice provide a reliable in vivo model of PSC and develop characteristic biliary inflammation and fibrosis. We tested the hypothesis that the tumor suppressor protein menin is implicated in the progression of liver fibrosis and that menin expression can be regulated in the liver via microRNA-24 (miR-24). MATERIALS AND METHODS: Menin expression was measured in human PSC and Mdr2(-/-) mice...
May 11, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28596252/success-in-bone-marrow-failure-novel-therapeutic-directions-based-on-the-immune-environment-of-myelodysplastic-syndromes
#20
REVIEW
Alyssa H Cull, Michael J Rauh
Myelodysplastic syndromes (MDS) are clonal neoplasms of aging that are associated with BM failure, related cytopenias, fatigue, susceptibility to infections, bruising, bleeding, a shortened lifespan, and a propensity for leukemic transformation. Most frail, elderly patients are not candidates for curative allogeneic BM transplantations and instead receive expectant management, supportive blood transfusions, or empirical, nontargeted therapy. It has been known for some time that MDS arises in an abnormal BM immune environment; however, connections have only recently been established with recurring MDS-associated mutations...
June 8, 2017: Journal of Leukocyte Biology
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