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https://www.readbyqxmd.com/read/29793181/exogenously-applied-spermidine-alleviates-photosynthetic-inhibition-under-drought-stress-in-maize-zea-mays-l-seedlings-associated-with-changes-in-endogenous-polyamines-and-phytohormones
#1
Lijie Li, Wanrong Gu, Jing Li, Congfeng Li, Tenglong Xie, Danyang Qu, Yao Meng, Caifeng Li, Shi Wei
Drought stress (DS) is a major environmental factor limiting plant growth and crop productivity worldwide. It has been established that exogenous spermidine (Spd) stimulates plant tolerance to DS. The effects of exogenous Spd on plant growth, photosynthetic performance, and chloroplast ultrastructure as well as changes in endogenous polyamines (PAs) and phytohormones were investigate in DS-resistant (Xianyu 335) and DS-sensitive (Fenghe 1) maize seedlings under well-watered and DS treatments. Exogenous Spd alleviated the stress-induced reduction in growth, photosynthetic pigment content, photosynthesis rate (Pn ) and photochemical quenching (qP ) parameters, including the maximum photochemistry efficiency of photosystem II (PSII) (Fv /Fm ), PSII operating efficiency (ФPSII), and qP coefficient...
May 15, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29793033/expression-of-anabaena-sensory-rhodopsin-is-influenced-by-different-codons-of-seven-residues-at-the-n-terminal-region
#2
Otgontuya Tsogbadrakh, Ah Reum Choi, Kwang-Hwan Jung
Microbial rhodopsins are well-known seven-transmembrane proteins that have been extensively studied for their structure and function. These retinal-binding proteins can be divided into two types. Type I is microbial rhodopsin, and type II (visual pigment) is expressed mostly in mammalian eyes. The two primary functions of type I rhodopsin are ion pumping activity and sensory transduction. Anabaena sensory rhodopsin (ASR) is a microbial rhodopsin with a specific function of photosensory transduction. ASR is expressed at moderate levels in Escherichia coli, but its expression level is lower compared to the general green light absorbing proteorhodopsin (GPR)...
May 21, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29792861/nlgn3-protects-retinal-cells-from-h-2-o-2-via-activating-nrf2-signaling
#3
Xiu-Miao Li, Dan Huang, Qing Yu, Jin Yao, Jian Yang
Intensified oxidative stress can cause severe damage to human retinal pigment epithelium (RPE) cells and retinal ganglion cells (RGCs). The potential effect of neuroligin-3 (NLGN3) against the process is studied here. Our results show that NLGN3 efficiently inhibited hydrogen peroxide (H2 O2 )-induced death and apoptosis in human RPE cells and RGCs. H2 O2 -induced reactive oxygen species (ROS) production, lipid peroxidation and DNA damage in retinal cells were alleviated by NLGN3. NLGN3 activated nuclear-factor-E2-related factor 2 (Nrf2) signaling, enabling Nrf2 protein stabilization, nuclear translocation and expression of key anti-oxidant enzymes (HO1, NOQ1 and GCLC) in RPE cells and RGCs...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792731/apr3-modulates-oxidative-stress-and-mitochondrial-function-in-arpe-19-cells
#4
Yuan Li, Xuan Zou, Jing Gao, Ke Cao, Zhihui Feng, Jiankang Liu
Impairment of retinal pigment epithelial (RPE) cells is considered a key contributor to the development of age-related macular degeneration. Apoptosis-related protein 3 (APR3) was recently discovered after treatment with all- trans retinoic acid, a pivotal molecule in RPE cells. However, the function of APR3 remains poorly understood. In the present study, we found that APR3 could interact with nuclear factor (erythroid-derived 2)-like 2, which is a regulator of phase II enzymes, and that knockdown of APR3 promoted nuclear factor (erythroid-derived 2)-like 2 nuclear translocation and activated expression of phase II enzymes, which was accompanied by improved redox status and mitochondrial activity...
May 24, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29792697/association-of-type-2-diabetes-with-submicron-titanium-dioxide-crystals-in-the-pancreas
#5
Adam Heller, Karalee Jarvis, Sheryl S Coffman
Pigment-grade titanium dioxide (TiO2) of 200-300 nm particle diameter is one of the most widely used submicron-sized particle materials. Inhaled and ingested TiO2 particles are known to enter the bloodstream, to be phagocytized by macrophages and neutrophils, to be inflammatory, and to activate the NLRP3 inflammasome. In this pilot study of 11 pancreatic specimens, 8 of the type 2 diabetic pancreas and 3 of the non-diabetic pancreas, we show that 110±70 nm average diameter TiO2 crystals abound in the type 2 diabetic pancreas, but not in the non-diabetic pancreas...
May 24, 2018: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29792438/collimonins-a-d-unstable-polyynes-with-antifungal-or-pigmentation-activities-from-the-fungus-feeding-bacterium-collimonas-fungivorans-ter331
#6
Kenji Kai, Mai Sogame, Fumie Sakurai, Norihiro Nasu, Makoto Fujita
The isolation and structure elucidation of collimonins A-D (1-4) from the fungus-feeding bacterium Collimonas fungivorans Ter331 are reported. Collimonins are new derivatives of polyoxygenated hexadecanoic acid, including an ene-triyne moiety. Their absolute configurations were fully determined by combining spectroscopic, chemical, and crystalline sponge methods. Collimonins showed antifungal or pigmentation activities against the fungus Aspergillus niger ATCC 9029.
May 24, 2018: Organic Letters
https://www.readbyqxmd.com/read/29792311/pedf-regulates-plasticity-of-a-novel-lipid-mtoc-axis-in-prostate-cancer-associated-fibroblasts
#7
Francesca Nardi, Philip Fitchev, Omar E Franco, Jelena Ivanisevic, Adrian Scheibler, Simon W Hayward, Charles B Brendler, Michael A Welte, Susan E Crawford
Prostate tumors make metabolic adaptations to ensure adequate energy and amplify cell cycle regulators such as centrosomes to sustain their proliferative capacity. It is not known whether cancer associated fibroblasts (CAFs) undergo metabolic re-programming. We postulated that CAFs augment lipid storage and amplify centrosomal or non-centrosomal microtubule organizing centers (MTOCs) through a pigment epithelium-derived factor (PEDF)-dependent lipid-MTOC signaling axis. Primary normal human prostate fibroblasts (NFs) and CAFs were evaluated for lipid content, triacylglycerol-regulating proteins, MTOC number and distribution...
May 23, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29791603/evaluation-instruments-for-quality-of-life-related-to-melasma-an-integrative-review
#8
Camila Fernandes Pollo, Silmara Meneguin, Helio Amante Miot
The aim of this study was to analyze scientific production concerning the validation and cultural adaptation of quality of life evaluation instruments for patients with melasma and to offer a critical reflection on these methods. A literature review was performed based on a search of the Web of Science, Bireme, PubMed, Elsevier Scopus, and Google Scholar databases. All published articles from indexed periodicals in these electronic databases up to December 2015 were included. Eight articles were identified, of which only one (12...
May 21, 2018: Clinics
https://www.readbyqxmd.com/read/29791447/development-of-an-rp-uhplc-pda-method-for-quantification-of-free-gossypol-in-cottonseed-cake-and-fungal-treated-cottonseed-cake
#9
Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio de Aquino Ribeiro, Felix Gonçalves de Siqueira, Robert Neil Gerard Miller, Simone Mendonça
Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated...
2018: PloS One
https://www.readbyqxmd.com/read/29790967/common-genetic-basis-of-eye-and-pigment-loss-in-two-distinct-cave-populations-of-the-isopod-crustacean-asellus-aquaticus
#10
Cassandra Re, Žiga Fišer, Justin Perez, Allyson Tacdol, Peter Trontelj, Meredith E Protas
Repeated evolution of similar phenotypes is a widespread phenomenon found throughout the living world and it can proceed through the same or different genetic mechanisms. Cave animals with their convergent traits such as eye and pigment loss, as well as elongated appendages, are a striking example of the evolution of similar phenotypes. Yet, few cave species are amenable to genetic crossing and mapping techniques making it challenging to determine the genetic mechanisms causing their similar phenotypes. To address this limitation, we have been developing Asellus aquaticus, a freshwater isopod crustacean, as a genetic model...
May 21, 2018: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/29790147/sensitivity-and-specificity-of-ex-vivo-dermatoscopy-a-case-series
#11
Danijela Dobrosavljevic, Dimitrije Brasanac, Sofija Glumac, Sanja Radojevic, Lidija Matija, Dejana Stanisavljevic
BACKGROUND: Sensitivity and specificity of ex vivo dermatoscopy (EVD) for malignancy detection of skin tumors is unknown. We sought to assess whether the use of EVD could be a useful adjunct to histopathological diagnosis of pigmented skin tumors, including cases where complete clinical information is inadequate or missing. MATERIALS AND METHODS: EVD was performed on 195 excised, formalin-fixed pigmented skin tumors. RESULTS: Of 183 eligible lesions, 104 (56...
May 23, 2018: International Journal of Dermatology
https://www.readbyqxmd.com/read/29790014/biochemical-profile-of-non-enzymatic-stress-markers-in-the-plant-species-urginea-maritima-in-a-mediterranean-natural-reserve-exposed-to-oxidative-stress
#12
Yara Khairallah, Tarek Houri, Bilal Osta, Dany Romanos, Georges Haddad
Protected areas decrease degrading natural ecosystems due to pollution such as air pollution. In 1981, the inhabitants founded Bentael natural reserve in Byblos, Lebanon, to secure their region against urbanization projects, like the recently constructed road that threatens the biodiversity of the reserve. This study was conducted to determine the oxidative stress resulting from this pollution and that menaces 360 floral species among them a rare species "Urginea maritima." In this research, the biomonitoring approach was experienced to assess the oxidative stress...
May 22, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29789708/combined-genotyping-microbial-diversity-and-metabolite-profiling-studies-on-farmed-mytilus-spp-from-kiel-fjord
#13
Caroline Utermann, Delphine Parrot, Corinna Breusing, Heiko Stuckas, Tim Staufenberger, Martina Blümel, Antje Labes, Deniz Tasdemir
The blue mussel Mytilus is a popular food source with high economical value. Species of the M. edulis complex (M. edulis, M. galloprovincialis and M. trossulus) hybridise whenever their geographic ranges overlap posing difficulties to species discrimination, which is important for blue mussel aquaculture. The aim of this study was to determine the genetic structure of farmed blue mussels in Kiel Fjord. Microbial and metabolic profile patterns were studied to investigate a possible dependency on the genotype of the bivalves...
May 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29789660/multicolor-tm-imaging-in-combined-hamartoma-of-the-retina-and-retinal-pigment-epithelium
#14
Konstantinos Kaprinis, Hannaa Bobat, Gabriella De Salvo
PURPOSE: To describe the principle characteristics of combined hamartoma of the retina and retinal pigment epithelium ('combined harmatoma') on MultiColorTM imaging and evaluate its role as a diagnostic and management tool. METHODS: Retrospective, observational case series comprising three patients with unilateral, juxtapapillary combined harmatoma. Complete ophthalmic examination was performed. MultiColorTM , including green reflectance (GR) and near infra-red reflectance (NIRR), optical coherence tomography (OCT) and autofluorescence (AF) imaging were obtained (Heidelberg Spectralis)...
May 23, 2018: Eye
https://www.readbyqxmd.com/read/29789643/changes-in-pituitary-gene-expression-may-underlie-multiple-domesticated-traits-in-chickens
#15
Amir Fallahshahroudi, Pia Løtvedt, Johan Bélteky, Jordi Altimiras, Per Jensen
Domesticated animals share a unique set of morphological and behavioral traits, jointly referred to as the domesticated phenotype. Striking similarities amongst a range of unrelated domesticated species suggest that similar regulatory mechanisms may underlie the domesticated phenotype. These include color pattern, growth, reproduction, development and stress response. Although previous studies have focused on the brain to find mechanisms underlying domestication, the potential role of the pituitary gland as a target of domestication is highly overlooked...
May 22, 2018: Heredity
https://www.readbyqxmd.com/read/29788869/advances-in-the-tyrosinase-inhibitors-from-plant-source
#16
Marco Bonesi, Jianbo Xiao, Rosa Tundis, Francesca Aiello, Vincenzo Sicari, Monica R Loizzo
Tyrosinase is a multifunctional copper-containing oxidase which catalyses the oxidation of tyrosine to produce melanin. The alteration in melanin biosynthesis occurs in many diseases. The pigment has a protecting role against skin photo-carcinogenesis, but anomalous melanin pigmentation is an aesthetic problem in human beings. Moreover, the formation of neuromelanin in human brain could contribute to the neurodegeneration associated with Parkinson's disease. Finally, tyrosinase is also responsible of undesired browning in fruits and vegetables...
May 21, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29788760/regression-patterns-of-choroidal-melanoma-after-palladium-103-103-pd-plaque-brachytherapy
#17
Abhilasha Maheshwari, Paul T Finger
PURPOSE: To describe the patterns of regression of choroidal melanoma after treatment with plaque brachytherapy. METHODS: Retrospective interventional case series including 170 consecutive patients treated with 103 Pd eye plaque radiation for choroidal melanoma. Outcome measures were changes in tumor thickness, surface characteristics, tumor vascularity, ultrasonography, fluorescein angiography, optical coherence tomography, and histopathology. RESULTS: The mean initial tumor thickness was 3...
May 1, 2018: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/29788682/immediate-breast-reconstruction-following-nipple-sparing-mastectomy-in-an-asian-population-aesthetic-outcomes-and-mitigating-nipple-areolar-complex-necrosis
#18
Wan-Sze Pek, Bien-Keem Tan, Yvonne Ying Ru Ng, Veronique Kiak Mien Tan, Mohamed Zulfikar Rasheed, Benita Kiat Tee Tan, Kong Wee Ong, Yee Siang Ong
BACKGROUND: Nipple-sparing mastectomies (NSMs) are increasingly performed to obtain the best aesthetic and psychological outcomes in breast cancer treatment. However, merely preserving the nipple-areolar complex (NAC) does not guarantee a good outcome. Darkly pigmented NACs and a tendency for poor scarring outcomes are particular challenges when treating Asian patients. Herein, we review the reconstructive outcomes following NSM at Singapore General Hospital. METHODS: All breasts reconstructed following NSM over an 11-year period from 2005 to 2015 were reviewed...
May 2018: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/29788491/lowering-the-recurrence-rate-in-pigmented-villonodular-synovitis-a-series-of-120-resections
#19
Carl Ferdinand Capellen, Reinhold Tiling, Alexander Klein, Andrea Baur-Melnyk, Thomas Knösel, Christof Birkenmaier, Falk Roeder, Volkmar Jansson, Hans Roland Dürr
Objectives: Tenosynovial giant-cell tumour or pigmented villonodular synovitis is an aggressive synovial proliferative disease, with the knee joint being the most commonly affected joint. The mainstay of therapy is surgical resection. The aim of this study was to evaluate the main patient characteristics, treatment and outcomes in a large single-centre retrospective study, focusing on meticulous aggressive open surgical procedures. Methods: From 1996 through 2014, 122 surgical interventions were performed in 105 patients...
May 16, 2018: Rheumatology
https://www.readbyqxmd.com/read/29788294/epigallocatechin-3-gallate-protected-vanadium-induced-eggshell-depigmentation-via-p38mapk-nrf2-ho-1-signaling-pathway-in-laying-hens
#20
J Wang, Z Yuan, K Zhang, X Ding, S Bai, Q Zeng, H Peng, P Celi
It has been demonstrated that tea polyphenol (TP) epigallocatechin-3-gallate (EGCG) can confer protection against vanadium (V) toxicity in laying hens; however, our understanding of the molecular mechanisms beyond this effect are still limited. In this study, 360 hens were randomly assigned to the 3 groups to study whether the potential mechanism P38MAPK-Nrf2/HO-1 signaling pathway is involved in the protective effect of EGCG on eggshell pigmentation in vanadium challenged laying hens. Treatments included a control group, a 10 mg/kg V (V10), and a V10 plus 130 mg/kg of EGCG group (V10+EGCG130)...
May 19, 2018: Poultry Science
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