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https://www.readbyqxmd.com/read/29627711/simple-dna-extraction-of-urine-samples-effects-of-storage-temperature-and-storage-time
#1
Huey Hian Ng, Hwee Chen Ang, See Ying Hoe, Mae-Lynn Lim, Hua Eng Tai, Richard Choon Hock Soh, Christopher Kiu-Choong Syn
Urine samples are commonly analysed in cases with suspected illicit drug consumption. In events of alleged sample mishandling, urine sample source identification may be necessary. A simple DNA extraction procedure suitable for STR typing of urine samples was established on the Promega Maxwell® 16 paramagnetic silica bead platform. A small sample volume of 1.7mL was used. Samples were stored at room temperature, 4°C and -20°C for 100days to investigate the influence of storage temperature and time on extracted DNA quantity and success rate of STR typing...
March 29, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29538381/taxonomy-based-on-science-is-necessary-for-global-conservation
#2
Scott A Thomson, Richard L Pyle, Shane T Ahyong, Miguel Alonso-Zarazaga, Joe Ammirati, Juan Francisco Araya, John S Ascher, Tracy Lynn Audisio, Valter M Azevedo-Santos, Nicolas Bailly, William J Baker, Michael Balke, Maxwell V L Barclay, Russell L Barrett, Ricardo C Benine, James R M Bickerstaff, Patrice Bouchard, Roger Bour, Thierry Bourgoin, Christopher B Boyko, Abraham S H Breure, Denis J Brothers, James W Byng, David Campbell, Luis M P Ceríaco, István Cernák, Pierfilippo Cerretti, Chih-Han Chang, Soowon Cho, Joshua M Copus, Mark J Costello, Andras Cseh, Csaba Csuzdi, Alastair Culham, Guillermo D'Elía, Cédric d'Udekem d'Acoz, Mikhail E Daneliya, René Dekker, Edward C Dickinson, Timothy A Dickinson, Peter Paul van Dijk, Klaas-Douwe B Dijkstra, Bálint Dima, Dmitry A Dmitriev, Leni Duistermaat, John P Dumbacher, Wolf L Eiserhardt, Torbjørn Ekrem, Neal L Evenhuis, Arnaud Faille, José L Fernández-Triana, Emile Fiesler, Mark Fishbein, Barry G Fordham, André V L Freitas, Natália R Friol, Uwe Fritz, Tobias Frøslev, Vicki A Funk, Stephen D Gaimari, Guilherme S T Garbino, André R S Garraffoni, József Geml, Anthony C Gill, Alan Gray, Felipe G Grazziotin, Penelope Greenslade, Eliécer E Gutiérrez, Mark S Harvey, Cornelis J Hazevoet, Kai He, Xiaolan He, Stephan Helfer, Kristofer M Helgen, Anneke H van Heteren, Francisco Hita Garcia, Norbert Holstein, Margit K Horváth, Peter H Hovenkamp, Wei Song Hwang, Jaakko Hyvönen, Melissa B Islam, John B Iverson, Michael A Ivie, Zeehan Jaafar, Morgan D Jackson, J Pablo Jayat, Norman F Johnson, Hinrich Kaiser, Bente B Klitgård, Dániel G Knapp, Jun-Ichi Kojima, Urmas Kõljalg, Jenő Kontschán, Frank-Thorsten Krell, Irmgard Krisai-Greilhuber, Sven Kullander, Leonardo Latella, John E Lattke, Valeria Lencioni, Gwilym P Lewis, Marcos G Lhano, Nathan K Lujan, Jolanda A Luksenburg, Jean Mariaux, Jader Marinho-Filho, Christopher J Marshall, Jason F Mate, Molly M McDonough, Ellinor Michel, Vitor F O Miranda, Mircea-Dan Mitroiu, Jesús Molinari, Scott Monks, Abigail J Moore, Ricardo Moratelli, Dávid Murányi, Takafumi Nakano, Svetlana Nikolaeva, John Noyes, Michael Ohl, Nora H Oleas, Thomas Orrell, Barna Páll-Gergely, Thomas Pape, Viktor Papp, Lynne R Parenti, David Patterson, Igor Ya Pavlinov, Ronald H Pine, Péter Poczai, Jefferson Prado, Divakaran Prathapan, Richard K Rabeler, John E Randall, Frank E Rheindt, Anders G J Rhodin, Sara M Rodríguez, D Christopher Rogers, Fabio de O Roque, Kevin C Rowe, Luis A Ruedas, Jorge Salazar-Bravo, Rodrigo B Salvador, George Sangster, Carlos E Sarmiento, Dmitry S Schigel, Stefan Schmidt, Frederick W Schueler, Hendrik Segers, Neil Snow, Pedro G B Souza-Dias, Riaan Stals, Soili Stenroos, R Douglas Stone, Charles F Sturm, Pavel Štys, Pablo Teta, Daniel C Thomas, Robert M Timm, Brian J Tindall, Jonathan A Todd, Dagmar Triebel, Antonio G Valdecasas, Alfredo Vizzini, Maria S Vorontsova, Jurriaan M de Vos, Philipp Wagner, Les Watling, Alan Weakley, Francisco Welter-Schultes, Daniel Whitmore, Nicholas Wilding, Kipling Will, Jason Williams, Karen Wilson, Judith E Winston, Wolfgang Wüster, Douglas Yanega, David K Yeates, Hussam Zaher, Guanyang Zhang, Zhi-Qiang Zhang, Hong-Zhang Zhou
No abstract text is available yet for this article.
March 2018: PLoS Biology
https://www.readbyqxmd.com/read/29494593/the-epidemiology-of-adolescents-living-with-perinatally-acquired-hiv-a-cross-region-global-cohort-analysis
#3
Amy L Slogrove, Michael Schomaker, Mary-Ann Davies, Paige Williams, Suna Balkan, Jihane Ben-Farhat, Nancy Calles, Kulkanya Chokephaibulkit, Charlotte Duff, Tanoh François Eboua, Adeodata Kekitiinwa-Rukyalekere, Nicola Maxwell, Jorge Pinto, George Seage, Chloe A Teasdale, Sebastian Wanless, Josiane Warszawski, Kara Wools-Kaloustian, Marcel Yotebieng, Venessa Timmerman, Intira J Collins, Ruth Goodall, Colette Smith, Kunjal Patel, Mary Paul, Diana Gibb, Rachel Vreeman, Elaine J Abrams, Rohan Hazra, Russell Van Dyke, Linda-Gail Bekker, Lynne Mofenson, Marissa Vicari, Shaffiq Essajee, Martina Penazzato, Gabriel Anabwani, Edith Q Mohapi, Peter N Kazembe, Makhosazana Hlatshwayo, Mwita Lumumba, Tessa Goetghebuer, Claire Thorne, Luisa Galli, Annemarie van Rossum, Carlo Giaquinto, Magdalena Marczynska, Laura Marques, Filipa Prata, Luminita Ene, Liubov Okhonskaia, Pablo Rojo, Claudia Fortuny, Lars Naver, Christoph Rudin, Sophie Le Coeur, Alla Volokha, Vanessa Rouzier, Regina Succi, Annette Sohn, Azar Kariminia, Andrew Edmonds, Patricia Lelo, Samuel Ayaya, Patricia Ongwen, Laura F Jefferys, Sam Phiri, Mwangelwa Mubiana-Mbewe, Shobna Sawry, Lorna Renner, Mariam Sylla, Mark J Abzug, Myron Levin, James Oleske, Miriam Chernoff, Shirley Traite, Murli Purswani, Ellen G Chadwick, Ali Judd, Valériane Leroy
BACKGROUND: Globally, the population of adolescents living with perinatally acquired HIV (APHs) continues to expand. In this study, we pooled data from observational pediatric HIV cohorts and cohort networks, allowing comparisons of adolescents with perinatally acquired HIV in "real-life" settings across multiple regions. We describe the geographic and temporal characteristics and mortality outcomes of APHs across multiple regions, including South America and the Caribbean, North America, Europe, sub-Saharan Africa, and South and Southeast Asia...
March 2018: PLoS Medicine
https://www.readbyqxmd.com/read/29140927/clinical-trial-designs-and-models-for-analgesic-medications-for-acute-pain-in-neonates-infants-toddlers-children-and-adolescents-acttion-recommendations
#4
Gary A Walco, Ernest A Kopecky, Steven J Weisman, Jennifer Stinson, Bonnie Stevens, Paul J Desjardins, Charles B Berde, Elliot J Krane, Kanwaljeet Js Anand, Myron Yaster, Carlton D Dampier, Robert H Dworkin, Ian Gilron, Anne M Lynn, Lynne G Maxwell, Srinivasa Raja, Bernard Schachtel, Dennis C Turk
Clinical trials to test the safety and efficacy of analgesics across all pediatric age cohorts are needed to avoid inappropriate extrapolation of adult data to children. However, the selection of acute pain models and trial design attributes to maximize assay sensitivity, by pediatric age cohort, remains problematic. Acute pain models used for drug treatment trials in adults are not directly applicable to the pediatric age cohorts - neonates, infants, toddlers, children, and adolescents. Developmental maturation of metabolic enzymes in infants and children must be taken into consideration when designing trials to test analgesic treatments for acute pain...
November 13, 2017: Pain
https://www.readbyqxmd.com/read/28902771/attention-and-visual-motor-integration-in-young-children-with-uncorrected-hyperopia
#5
MULTICENTER STUDY
Marjean Taylor Kulp, Elise Ciner, Maureen Maguire, Maxwell Pistilli, T Rowan Candy, Gui-Shuang Ying, Graham Quinn, Lynn Cyert, Bruce Moore
SIGNIFICANCE: Among 4- and 5-year-old children, deficits in measures of attention, visual-motor integration (VMI) and visual perception (VP) are associated with moderate, uncorrected hyperopia (3 to 6 diopters [D]) accompanied by reduced near visual function (near visual acuity worse than 20/40 or stereoacuity worse than 240 seconds of arc). PURPOSE: To compare attention, visual motor, and visual perceptual skills in uncorrected hyperopes and emmetropes attending preschool or kindergarten and evaluate their associations with visual function...
October 2017: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/28844645/direct-electrical-stimulation-in-the-human-brain-disrupts-melody-processing
#6
Frank E Garcea, Benjamin L Chernoff, Bram Diamond, Wesley Lewis, Maxwell H Sims, Samuel B Tomlinson, Alexander Teghipco, Raouf Belkhir, Sarah B Gannon, Steve Erickson, Susan O Smith, Jonathan Stone, Lynn Liu, Trenton Tollefson, John Langfitt, Elizabeth Marvin, Webster H Pilcher, Bradford Z Mahon
Prior research using functional magnetic resonance imaging (fMRI) [1-4] and behavioral studies of patients with acquired or congenital amusia [5-8] suggest that the right posterior superior temporal gyrus (STG) in the human brain is specialized for aspects of music processing (for review, see [9-12]). Intracranial electrical brain stimulation in awake neurosurgery patients is a powerful means to determine the computations supported by specific brain regions and networks [13-21] because it provides reversible causal evidence with high spatial resolution (for review, see [22, 23])...
September 11, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28507124/thiophene-antibacterials-that-allosterically-stabilize-dna-cleavage-complexes-with-dna-gyrase
#7
Pan F Chan, Thomas Germe, Benjamin D Bax, Jianzhong Huang, Reema K Thalji, Eric Bacqué, Anna Checchia, Dongzhao Chen, Haifeng Cui, Xiao Ding, Karen Ingraham, Lynn McCloskey, Kaushik Raha, Velupillai Srikannathasan, Anthony Maxwell, Robert A Stavenger
A paucity of novel acting antibacterials is in development to treat the rising threat of antimicrobial resistance, particularly in Gram-negative hospital pathogens, which has led to renewed efforts in antibiotic drug discovery. Fluoroquinolones are broad-spectrum antibacterials that target DNA gyrase by stabilizing DNA-cleavage complexes, but their clinical utility has been compromised by resistance. We have identified a class of antibacterial thiophenes that target DNA gyrase with a unique mechanism of action and have activity against a range of bacterial pathogens, including strains resistant to fluoroquinolones...
May 30, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28449302/prescribing-safety-assessment-2016-delivery-of-a-national-prescribing-assessment-to-7343-uk-final-year-medical-students
#8
Simon R J Maxwell, Jamie J Coleman, Lynne Bollington, Celia Taylor, David J Webb
AIMS: Newly graduated doctors write a large proportion of prescriptions in UK hospitals but recent studies have shown that they frequently make prescribing errors. The prescribing safety assessment (PSA) has been developed as an assessment of competence in relation to prescribing and supervising the use of medicines. This report describes the delivery of the PSA to all UK final-year medical students in 2016 (PSA2016). METHODS: The PSA is a 2-hour online assessment comprising eight sections which cover various aspects of prescribing defined within the outcomes of undergraduate education identified by the UK General Medical Council...
October 2017: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28300512/the-hypnotic-induction-in-the-broad-scheme-of-hypnosis-a-sociocognitive-perspective
#9
REVIEW
Steven Jay Lynn, Reed Maxwell, Joseph P Green
Researchers and clinicians typically divide hypnosis into two distinct parts: the induction and the suggestions that follow. We suggest that this distinction is arbitrary and artificial. Different definitions of hypnosis ascribe different roles to the hypnotic induction, yet none clearly specifies the mechanisms that mediate or moderate subjective and behavioral responses to hypnotic suggestions. Researchers have identified few if any differences in responding across diverse hypnotic inductions, and surprisingly little research has focused on the specific ingredients that optimize responsiveness...
April 2017: American Journal of Clinical Hypnosis
https://www.readbyqxmd.com/read/28281916/prevalence-and-correlates-of-arrests-or-stops-for-drunk-driving-on-both-sides-of-the-u-s-mexico-border
#10
Lynn Wallisch, Sarah E Zemore, Guilherme Borges, Cheryl J Cherpitel, Jane C Maxwell
Risk for driving under the influence of alcohol (DUI) may be higher in U.S. and Mexico border cities as compared to nonborder cities in each country. We examine rates and correlates of self-reported DUI arrests or stops on both sides of the border, drawing on a large-scale survey of 4,796 Mexicans and Mexican Americans in border and nonborder cities of Texas and two states in Mexico. Findings varied by site and country and did not consistently show higher rates on the border. DUI prevention efforts should consider the heterogeneity of local conditions and needs...
March 10, 2017: Journal of Ethnicity in Substance Abuse
https://www.readbyqxmd.com/read/28238214/self-reported-substance-use-in-iraq-findings-from-the-iraqi-national-household-survey-of-alcohol-and-drug-use-2014
#11
Nesif Al-Hemiery, Rufaidah Dabbagh, Mushtaq T Hashim, Salih Al-Hasnawi, Ali Abutiheen, Emad A Abdulghani, Jawad K Al-Diwan, Neeraj Kak, Hala Al Mossawi, Jane Carlisle Maxwell, Mary-Lynn Brecht, Valerie Antonini, Albert Hasson, Richard A Rawson
AIMS: To estimate the prevalence of tobacco, alcohol and drug use in Iraq using data from the Iraqi National Household Survey of Alcohol and Drug Use (INHSAD). DESIGN: A cross-sectional survey was conducted using a multi-stage cluster sampling method. Trained surveyors conducted face-to-face household interviews. SETTING: Iraq, from April 2014 to December 2014. PARTICIPANTS: A total of 3200 adult, non-institutionalized Iraqi citizens residing across all 18 governorates of Iraq...
February 26, 2017: Addiction
https://www.readbyqxmd.com/read/28062336/comparative-safety-of-morphine-delivered-via-intravenous-route-vs-patient-controlled-analgesia-device-for-pediatric-inpatients
#12
Jennifer Faerber, Wenjun Zhong, Dingwei Dai, Avi Baehr, Lynne G Maxwell, Francis Wickham Kraemer, Chris Feudtner
CONTEXT: Although patient-controlled analgesia (PCA) is an effective pain control modality, there is a lack of large studies on PCA safety in pediatric patients. OBJECTIVES: This study compared the delivery of morphine either via intravenous route (morphine IV) or via PCA device (morphine PCA) on risk of cardiopulmonary resuscitation (CPR) and mechanical ventilation (MV) using a large administrative database. METHODS: We assembled a retrospective cohort of pediatric inpatients between five and 21 years old in 42 children's hospitals between 2007 and 2011 from the Pediatric Health Information System database...
May 2017: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/27768675/optimal-timing-of-surgical-procedures-in-pediatric-patients
#13
REVIEW
Riva R Ko, Teeda Pinyavat, Steven Stylianos, Sarah M Lambert, Richard C Anderson, Pamela F Gallin, Lynne G Maxwell, Christopher G Ward, Jayant K Deshpande, Constance S Houck
The Pediatric Anesthesia Neuro Development Assessment (PANDA) team at the Anesthesiology Department at Columbia University Medical Center held its fifth biennial symposium to discuss issues regarding potential neurotoxicity of anesthetic agents in pediatric patients. Overall optimal surgical timing as well as a "critical window" for surgery on a specialty specific basis are areas of focus for the American Academy of Pediatrics Surgical Advisory Panel. An ad hoc panel of pediatric surgical experts representing general surgery, urology, neurosurgery, and ophthalmology was assembled for this meeting and provided a dialogue focused on the benefits of early intervention versus potential anesthetic risk, addressing parental concerns, and the need for continued interdisciplinary collaboration in this area...
October 2016: Journal of Neurosurgical Anesthesiology
https://www.readbyqxmd.com/read/27532251/neonatal-anesthesia
#14
Stephanie A Black, Lynne G Maxwell
No abstract text is available yet for this article.
September 2016: Anesthesiology
https://www.readbyqxmd.com/read/27477769/visual-function-of-moderately-hyperopic-4-and-5-year-old-children-in-the-vision-in-preschoolers-hyperopia-in-preschoolers-study
#15
MULTICENTER STUDY
Elise B Ciner, Marjean Taylor Kulp, Maureen G Maguire, Maxwell Pistilli, T Rowan Candy, Bruce Moore, Gui-Shuang Ying, Graham Quinn, Gale Orlansky, Lynn Cyert
PURPOSE: To compare visual performance between emmetropic and uncorrected moderately hyperopic preschool-age children without strabismus or amblyopia. DESIGN: Cross-sectional study. METHODS: setting: Multicenter, institutional. patient or study population: Children aged 4 or 5 years. intervention or observation procedures: Visual functions were classified as normal or reduced for each child based on the 95% confidence interval for emmetropic individuals...
October 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27272582/association-between-a-single-general-anesthesia-exposure-before-age-36-months-and-neurocognitive-outcomes-in-later-childhood
#16
MULTICENTER STUDY
Lena S Sun, Guohua Li, Tonya L K Miller, Cynthia Salorio, Mary W Byrne, David C Bellinger, Caleb Ing, Raymond Park, Jerilynn Radcliffe, Stephen R Hays, Charles J DiMaggio, Timothy J Cooper, Virginia Rauh, Lynne G Maxwell, Ahrim Youn, Francis X McGowan
IMPORTANCE: Exposure of young animals to commonly used anesthetics causes neurotoxicity including impaired neurocognitive function and abnormal behavior. The potential neurocognitive and behavioral effects of anesthesia exposure in young children are thus important to understand. OBJECTIVE: To examine if a single anesthesia exposure in otherwise healthy young children was associated with impaired neurocognitive development and abnormal behavior in later childhood...
June 7, 2016: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/27022143/nf%C3%AE%C2%BAb1-is-essential-to-prevent-the-development-of-multiorgan-autoimmunity-by-limiting-il-6-production-in-follicular-b-cells
#17
Elisha de Valle, George Grigoriadis, Lorraine A O'Reilly, Simon N Willis, Mhairi J Maxwell, Lynn M Corcoran, Evelyn Tsantikos, Jasper K S Cornish, Kirsten A Fairfax, Ajithkumar Vasanthakumar, Mark A Febbraio, Margaret L Hibbs, Marc Pellegrini, Ashish Banerjee, Philip D Hodgkin, Axel Kallies, Fabienne Mackay, Andreas Strasser, Steve Gerondakis, Raffi Gugasyan
We examined the role of NFκB1 in the homeostasis and function of peripheral follicular (Fo) B cells. Aging mice lacking NFκB1 (Nfκb1(-/-)) develop lymphoproliferative and multiorgan autoimmune disease attributed in large part to the deregulated activity of Nfκb1(-/-)Fo B cells that produce excessive levels of the proinflammatory cytokine interleukin 6 (IL-6). Despite enhanced germinal center (GC) B cell differentiation, the formation of GC structures was severely disrupted in the Nfκb1(-/-)mice. Bone marrow chimeric mice revealed that the Fo B cell-intrinsic loss of NFκB1 led to the spontaneous generation of GC B cells...
April 4, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27009035/rapid-recovery-pathway-after-spinal-fusion-for-idiopathic-scoliosis
#18
Wallis T Muhly, Wudbhav N Sankar, Kelly Ryan, Annette Norton, Lynne G Maxwell, Theresa DiMaggio, Sharon Farrell, Rachel Hughes, Alex Gornitzky, Ron Keren, John J McCloskey, John M Flynn
BACKGROUND: Posterior spinal fusion (PSF) for adolescent idiopathic scoliosis (AIS) is associated with significant pain and prolonged hospitalization. There is evidence that early mobilization and multimodal analgesia can accelerate functional recovery and reduced length of stay (LOS). Using these principles, we implemented a quality improvement initiative to enable earlier functional recovery in our AIS-PSF population. METHODS: We designed and implemented a standardized rapid recovery pathway (RRP) with evidence-based management recommendations for children aged 10 to 21 years undergoing PSF for AIS...
April 2016: Pediatrics
https://www.readbyqxmd.com/read/26826748/uncorrected-hyperopia-and-preschool-early-literacy-results-of-the-vision-in-preschoolers-hyperopia-in-preschoolers-vip-hip-study
#19
MULTICENTER STUDY
Marjean Taylor Kulp, Elise Ciner, Maureen Maguire, Bruce Moore, Jill Pentimonti, Maxwell Pistilli, Lynn Cyert, T Rowan Candy, Graham Quinn, Gui-Shuang Ying
PURPOSE: To compare early literacy of 4- and 5-year-old uncorrected hyperopic children with that of emmetropic children. DESIGN: Cross-sectional. PARTICIPANTS: Children attending preschool or kindergarten who had not previously worn refractive correction. METHODS: Cycloplegic refraction was used to identify hyperopia (≥3.0 to ≤6.0 diopters [D] in most hyperopic meridian of at least 1 eye, astigmatism ≤1.5 D, anisometropia ≤1...
April 2016: Ophthalmology
https://www.readbyqxmd.com/read/26690879/functional-and-genomic-insights-into-the-pathogenesis-of-burkholderia-species-to-rice
#20
REVIEW
Lynn M Naughton, Shi-qi An, Ingyu Hwang, Shan-Ho Chou, Yong-Qiang He, Ji-Liang Tang, Robert P Ryan, J Maxwell Dow
A number of species of bacteria from the genus Burkholderia have been shown to be causal agents of diseases of rice. These diseases, caused by Burkholderia glumae, B. gladioli and B. plantarii, are becoming increasingly common across the globe. This is particularly so for B. glumae, whose ability to grow at elevated temperatures suggests that it may become a prevalent problem in an era of global warming. Despite the increasing threat to rice, relatively little is known about the virulence mechanisms employed by these pathogens...
March 2016: Environmental Microbiology
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