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https://www.readbyqxmd.com/read/28239997/effects-of-different-doses-of-silk-peptide-on-energy-metabolism-during-exercise-in-mice
#1
Suh Heajung, Kim Jisu, Park Jonghoon, Kim Bokyung, Lee Chi-Ho, Lim Kiwon
Purpose: This study was carried out to determine the optimal dose of silk peptide for enhancing fat metabolism during exercise. Methods: Fifty male ICR mice were randomly divided into five groups: Sed, SP0, SP200, SP400, and SP800. All SP mice underwent training by running on a treadmill 5 times a week for 2 weeks (20 m/min, 8° slope, 50 min/day for the first week and 25 m/min, 8° slope, 50 min/day at about 70-75% of maximum oxygen uptake for the second week)...
February 24, 2017: Journal of Exercise Nutrition & Biochemistry
https://www.readbyqxmd.com/read/28239404/impact-of-a-6-week-non-energy-restricted-ketogenic-diet-on-physical-fitness-body-composition-and-biochemical-parameters-in-healthy-adults
#2
Paul Urbain, Lena Strom, Lena Morawski, Anja Wehrle, Peter Deibert, Hartmut Bertz
BACKGROUND: The ketogenic diet (KD) is a very low-carbohydrate, high-fat and adequate-protein diet that without limiting calories induces different metabolic adaptations, eg, increased levels of circulating ketone bodies and a shift to lipid metabolism. Our objective was to assess the impact of a 6-week non-energy-restricted KD in healthy adults beyond cohorts of athletes on physical performance, body composition, and blood parameters. METHODS: Our single arm, before-and-after comparison study consisted of a 6-week KD with a previous preparation period including detailed instructions during classes and individual counselling by a dietitian...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28239013/recruitment-and-baseline-characteristics-of-participants-in-the-lifestyle-improvement-through-food-and-exercise-life-study
#3
Elizabeth Lynch, Laurin J Mack, Kelly Karavolos, Elizabeth Avery, Rebecca Liebman, Kathryn S Keim, Crystal M Glover, Leon Fogelfeld
African Americans experience poorer diabetes outcomes than non-Hispanic Whites. Few clinical trials of diabetes self-management interventions specifically target African Americans, perhaps due to well-documented barriers to recruitment in this population. This paper describes strategies used to successfully recruit 211 low-income African Americans from community clinics of a large, urban public hospital system to a randomized clinical trial of an 18-month diabetes self-management intervention. Diabetes-related physiological, psychosocial, and behavioral characteristics of the sample are reported...
2017: Journal of Health Care for the Poor and Underserved
https://www.readbyqxmd.com/read/28238810/defect-in-dermatan-sulfate-in-urine-of-patients-with-ehlers-danlos-syndrome-caused-by-a-chst14-d4st1-deficiency
#4
Shuji Mizumoto, Tomoki Kosho, Atsushi Hatamochi, Tomoko Honda, Tomomi Yamaguchi, Nobuhiko Okamoto, Noriko Miyake, Shuhei Yamada, Kazuyuki Sugahara
PURPOSE: Dermatan sulfate (DS) plays a number of roles in a wide range of biological activities such as cell signaling and tissue morphogenesis through interactions with various extracellular matrix proteins including collagen. Mutations in the carbohydrate sulfotransferase 14 gene (CHST14) encoding CHST14/dermatan 4-O-sulfotransferase-1 (D4ST1), which is responsible for the biosynthesis of DS, cause a recently delineated form of Ehlers-Danlos syndrome (EDS, musculocontractural type 1), an autosomal recessive connective tissue disorder characterized by congenital malformations (specific craniofacial features, and congenital multiple contractures) and progressive fragility-related complications (skin hyperextensibility, bruisability, and fragility with atrophic scars; recurrent dislocations; progressive talipes or spinal deformities; and large subcutaneous hematomas)...
February 23, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28238682/association-between-dietary-pattern-and-cardiometabolic-risk-in-children-and-adolescents-a-systematic-review
#5
REVIEW
Naruna Pereira Rocha, Luana Cupertino Milagres, Giana Zarbato Longo, Andréia Queiroz Ribeiro, Juliana Farias de Novaes
OBJECTIVE: To evaluate the association between dietary patterns and cardiometabolic risk factors in children and adolescents. DATA SOURCE: This article followed the recommendations of PRISMA, which aims to guide review publications in the health area. The article search strategy included searches in the electronic databases MEDLINE via PubMed, Scopus, and LILACS. There was no date limitation for publications. The descriptors were used in English according to MeSH and in Portuguese according to DeCS...
February 23, 2017: Jornal de Pediatria
https://www.readbyqxmd.com/read/28238618/metabolic-demands-and-replenishment-of-muscle-glycogen-after-a-rugby-league-match-simulation-protocol
#6
Warren J Bradley, Marcus P Hannon, Victoria Benford, James C Morehen, Craig Twist, Sam Shepherd, Matthew Cocks, Samuel G Impey, Robert G Cooper, James P Morton, Graeme L Close
OBJECTIVES: The metabolic requirements of a rugby league match simulation protocol and the timing of carbohydrate provision on glycogen re-synthesis in damaged muscle were examined. DESIGN: Fifteen (mean±SD: age 20.9±2.9 year, body-mass 87.3±14.1kg, height 177.4±6.0cm) rugby league (RL) players consumed a 6gkgday-1 CHO diet for 7-days, completed a time to exhaustion test (TTE) and a glycogen depletion protocol on day-3, a RL simulated-match protocol (RLMSP) on day-5 and a TTE on day-7...
February 15, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28238541/igg4-related-sclerosing-cholangitis-overlapping-with-autoimmune-hepatitis-report-of-a-case
#7
Hongyan Li, Li Sun, David R Brigstock, Lina Qi, Runping Gao
BACKGROUND: IgG4-related sclerosing cholangitis (IgG4-SC) is the biliary manifestation of IgG4-related disease (IgG4-RD) but the presence of IgG4-SC in the porta hepatis is difficult to differentiate from hilar cholangiocarcinoma (HCCA). IgG4-related autoimmune hepatitis (IgG4-related AIH) is extremely rare and it is not fully clear whether IgG4-related AIH is a hepatic manifestation of IgG4-RD or a subtype of AIH. CASE PRESENTATION: We present a rare case of a 52-year-old male who was admitted with obstructive jaundice and itchy skin...
February 10, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28237882/a-comparative-secretome-analysis-of-industrial-aspergillus-oryzae-and-its-spontaneous-mutant-zjgs-lz-21
#8
Yuanyuan Zhu, Xinle Liang, Hong Zhang, Wei Feng, Ye Liu, Fuming Zhang, Robert J Linhardt
Aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. In the present study, we performed a comparative secretome analysis of the industrial strain of Aspergillus oryzae 3.042 and its spontaneous mutantZJGS-LZ-21. One hundred and fifty two (152) differential protein spots were excised (p<0.05), and 25 proteins were identified. Of the identified proteins, 91.3% belonged to hydrolytic enzymes acting on carbohydrates or proteins. Consistent with their enzyme activities, the expression of 14 proteins involved in the degradation of cellulose, hemicellulose, starch and proteins, increased in the ZJGS-LZ-21isolate...
February 8, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28237649/protein-kinase-ck2%C3%AE-catalytic-subunit-ameliorates-diabetic-renal-inflammatory-fibrosis-via-nf-%C3%AE%C2%BAb-signaling-pathway
#9
Junying Huang, Zhiquan Chen, Jie Li, Qiuhong Chen, Jingyan Li, Wenyan Gong, Jiani Huang, Peiqing Liu, Heqing Huang
Activation of casein kinase 2 (CK2) is closely linked to the body disturbance of carbohydrate metabolism and inflammatory reaction. The renal chronic inflammatory reaction in the setting of diabetes is one of the important hallmarks of diabetic renal fibrosis. However, it remains unknown whether CK2 influences the process of diabetic renal fibrosis. The current study is aimed to investigate if CK2α ameliorates renal inflammatory fibrosis in diabetes via NF-κB pathway. To explore potential regulatory mechanism of CK2α, the expression and activity of CK2α, which were studied by plasmid transfection, selective inhibitor, small-interfering RNA (siRNA) and adenovirus infection in vitro or in vivo, were analyzed by means of western blotting (WB), dual luciferase reporter assay and electrophoretic mobility shift assay (EMSA)...
February 22, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28237643/adjunctive-role-of-preoperative-liver-magnetic-resonance-imaging-for-potentially-resectable-pancreatic-cancer
#10
Hyoung Woo Kim, Jong-Chan Lee, Kyu-Hyun Paik, Jingu Kang, Young Hoon Kim, Yoo-Seok Yoon, Ho-Seong Han, Jaihwan Kim, Jin-Hyeok Hwang
BACKGROUND: The adjunctive role of magnetic resonance imaging of the liver before pancreatic ductal adenocarcinoma has been unclear. We evaluated whether the combination of hepatic magnetic resonance imaging with multidetector computed tomography using a pancreatic protocol (pCT) could help surgeons select appropriate candidates and decrease the risk of early recurrence. METHODS: We retrospectively enrolled 167 patients in whom complete resection was achieved without grossly visible residual tumor; 102 patients underwent pCT alone (CT group) and 65 underwent both hepatic magnetic resonance imaging and pCT (magnetic resonance imaging group)...
February 22, 2017: Surgery
https://www.readbyqxmd.com/read/28237436/cationic-dendritic-starch-as-a-vehicle-for-photodynamic-therapy-and-sirna-co-delivery
#11
Sarah A Engelberth, Nadine Hempel, Magnus Bergkvist
Cationic enzymatically synthesized glycogen (cESG) is a naturally-derived, nano-scale carbohydrate dendrite that has shown promise as a cellular delivery vehicle owing to its flexibility in chemical modifications, biocompatibility and relative low cost. In the present work, cESG was modified and evaluated as a vehicle for tetraphenylporphinesulfonate (TPPS) in order to improve cellular delivery of this photosensitizer and investigate the feasibility of co-delivery with short interfering ribonucleic acid (siRNA)...
February 20, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28237316/metabolite-transport-across-the-mammalian-and-insect-brain-diffusion-barriers
#12
Astrid Weiler, Anne Volkenhoff, Helen Hertenstein, Stefanie Schirmeier
The nervous system in higher vertebrates is separated from the circulation by a layer of specialized endothelial cells. It protects the sensitive neurons from harmful blood-derived substances, high and fluctuating ion concentrations, xenobiotics or even pathogens. To this end, the brain endothelial cells and their interlinking tight junctions build an efficient diffusion barrier. A structurally analogous diffusion barrier exists in insects, where glial cell layers separate the hemolymph from the neural cells...
February 22, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28237295/carbohydrate-quality-and-quantity-affects-the-composition-of-the-red-blood-cell-fatty-acid-membrane-in-overweight-and-obese-individuals
#13
Simona Giardina, Aleix Sala-Vila, Pablo Hernández-Alonso, Carlos Calvo, Jordi Salas-Salvadó, Mònica Bulló
BACKGROUND: Cell membrane fatty acid (FA) composition may play a role in human metabolic diseases. However, the modulatory effect of nutrients other than fat is poorly explored. OBJECTIVE: To investigate the effect of moderate-carbohydrate diets with different glycemic indices (GI) and a low-fat diet (LF) on red blood cell (RBC) FA membrane composition. DESIGN: The RBC FA profile was measured in 87 subjects from the GLYNDIET study. Participants were randomly assigned to one of the following energy-restricted diet for 6 months: moderate-carbohydrate/low-GI diet (LGI, n = 31), moderate-carbohydrate/high-GI diet (HGI, n = 30) or LF-diet (n = 26)...
February 11, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28236479/glyphosate-binding-in-soil-as-revealed-by-sorption-experiments-and-quantum-chemical-modeling
#14
Peter Gros, Ashour Ahmed, Oliver Kühn, Peter Leinweber
The herbicide glyphosate (GLP) is supposed to be rapidly degraded or adsorbed strongly by soil solids but findings in soil years after application and concentrations in waters above legal limits question a harmless disappearance. Therefore, we conducted batch sorption experiments with 23 thoroughly characterized arable surface soils, correlated isotherm coefficients with numerous inorganic and organic soil parameters, and investigated GLP-SOM-complexes by quantum-chemical modeling. The Freundlich sorption model yielded the best fits, and coefficients Kf and nf were correlated positively with the contents of clay/silt...
February 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28236367/comparison-of-transferrin-isoform-analysis-by-capillary-electrophoresis-and-hplc-for-screening-congenital-disorders-of%C3%A2-glycosylation
#15
Mihika B Dave, Alpa J Dherai, Vrajesh P Udani, Anaita U Hegde, Neelu A Desai, Tester F Ashavaid
BACKGROUND: Transferrin, a major glycoprotein has different isoforms depending on the number of sialic acid residues present on its oligosaccharide chain. Genetic variants of transferrin as well as the primary (CDG) & secondary glycosylation defects lead to an altered transferrin pattern. Isoform analysis methods are based on charge/mass variations. We aimed to compare the performance of commercially available capillary electrophoresis CDT kit for diagnosing congenital disorders of glycosylation with our in-house optimized HPLC method for transferrin isoform analysis...
February 25, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28236296/different-types-of-dietary-advice-for-women-with-gestational-diabetes-mellitus
#16
REVIEW
Shanshan Han, Philippa Middleton, Emily Shepherd, Emer Van Ryswyk, Caroline A Crowther
BACKGROUND: Dietary advice is the main strategy for managing gestational diabetes mellitus (GDM). It remains unclear what type of advice is best. OBJECTIVES: To assess the effects of different types of dietary advice for women with GDM for improving health outcomes for women and babies. SEARCH METHODS: We searched Cochrane Pregnancy and Childbirth's Trials Register (8 March 2016), PSANZ's Trials Registry (22 March 2016) and reference lists of retrieved studies...
February 25, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28235871/metatranscriptomic-profiling-reveals-linkages-between-the-active-rumen-microbiome-and-feed-efficiency-in-beef-cattle
#17
Fuyong Li, Le Luo Guan
Exploring compositional and functional characteristics of the rumen microbiome can improve the understanding of its role in rumen function and feed efficiency. In this study, we applied metatranscriptomics to characterize the active rumen microbiomes of beef cattle with different feed efficiencies (efficient, n=10; inefficient, n=10) using total RNA sequencing. Active bacterial and archaeal compositions were estimated based on 16S rRNAs, and active microbial metabolic functions including carbohydrate-active enzymes (CAZymes) were assessed based on mRNAs from the same metatranscriptomic datasets...
February 24, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28235823/an-induced-chromosomal-translocation-in-soybean-disrupts-a-kasi-ortholog-and-is-associated-with-a-high-sucrose-and-low-oil-seed-phenotype
#18
Austin A Dobbels, Jean-Michel Michno, Benjamin W Campbell, Kamaldeep S Virdi, Adrian O Stec, Gary J Muehlbauer, Seth L Naeve, Robert M Stupar
Mutagenesis is a useful tool in many crop species to induce heritable genetic variability for trait improvement and gene discovery. In this study, forward screening of a soybean fast neutron mutant population identified an individual that produced seed with nearly twice the amount of sucrose (8.1% on dry matter basis) and less than half the amount of oil (8.5% on dry matter basis) as compared to wild-type. Bulked segregant analysis, comparative genomic hybridization, and genome resequencing were used to associate the seed composition phenotype with a reciprocal translocation between chromosomes 8 and 13...
February 24, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28235467/identification-and-characterization-of-a-novel-starch-branching-enzyme-from-the-picoalgae-ostreococcus-tauri
#19
Nicolas Hedin, Julieta Barchiesi, Diego F Gomez-Casati, Alberto A Iglesias, Miguel A Ballicora, María V Busi
Starch branching enzyme is a highly conserved protein from plants to algae. This enzyme participates in starch granule assembly by the addition of α-1,6-glucan branches to the α-1,4-polyglucans. This modification determines the structure of amylopectin thus arranging the final composition of the starch granule. Herein, we describe the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri. Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE)...
February 21, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28231561/structural-analysis-of-rebaudioside-a-derivatives-obtained-by-lactobacillus-reuteri-180-glucansucrase-catalyzed-trans-%C3%AE-glucosylation
#20
Gerrit J Gerwig, Evelien M Te Poele, Lubbert Dijkhuizen, Johannis P Kamerling
The wild-type Gtf180-ΔN glucansucrase enzyme from Lactobacillus reuteri 180 was found to catalyze the α-glucosylation of the steviol glycoside rebaudioside A, using sucrose as glucosyl donor in a transglucosylation process. Structural analysis of the formed products by MALDI-TOF mass spectrometry, methylation analysis and NMR spectroscopy showed that rebaudioside A is specifically α-d-glucosylated at the steviol C-19 β-d-glucosyl moiety (55% conversion). The main product is a mono-(α1 → 6)-glucosylated derivative (RebA-G1)...
January 31, 2017: Carbohydrate Research
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