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Phloridzin

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https://www.readbyqxmd.com/read/28279980/glucose-transporter-4-glut4-is-not-necessary-for-overload-induced-glucose-uptake-or-hypertrophic-growth-in-mouse-skeletal-muscle
#1
Shawna L McMillin, Denise L Schmidt, Barbara B Kahn, Carol A Witczak
Glucose transporter 4 (GLUT4) is necessary for acute insulin- and contraction-induced skeletal muscle glucose uptake, but its role in chronic muscle loading (overload)-induced glucose uptake is unknown. Our goal was to determine if GLUT4 is required for overload-induced glucose uptake. Overload was induced in mouse plantaris muscle by unilateral synergist ablation. After 5 days, muscle weights and ex vivo [(3)H]-2-deoxy-D-glucose uptake were assessed. Overload-induced muscle glucose uptake and hypertrophic growth were not impaired in muscle-specific GLUT4 knockout mice, demonstrating that GLUT4 is not necessary for these processes...
March 9, 2017: Diabetes
https://www.readbyqxmd.com/read/28241438/lycium-barbarum-l-polysaccharide-lbp-reduces-glucose-uptake-via-down-regulation-of-sglt-1-in-caco2-cell
#2
Huizhen Cai, Xiaohui Yang, Qian Cai, Binbin Ren, Hongyan Qiu, Zhiqing Yao
Lycium barbarum L. polysaccharide (LBP) is prepared from Lycium barbarum L. (L. barbarum), which is a traditional Chinese medicine. LPB has been shown to have hypoglycemic effects. In order to gain some mechanistic insights on the hypoglycemic effects of LBP, we investigated the uptake of LBP and its effect on glucose absorption in the human intestinal epithelial cell line Caco2 cell. The uptake of LBP through Caco2 cell monolayer was time-dependent and was inhibited by phloridzin, a competitive inhibitor of SGLT-1...
February 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28192456/the-flavanone-homoeriodictyol-increases-sglt-1-mediated-glucose-uptake-but-decreases-serotonin-release-in-differentiated-caco-2-cells
#3
Barbara Lieder, Julia Katharina Hoi, Ann-Katrin Holik, Katrin Geissler, Joachim Hans, Barbara Friedl, Kathrin Liszt, Gerhard E Krammer, Jakob P Ley, Veronika Somoza
Flavanoids and related polyphenols, among them hesperitin, have been shown to modulate cellular glucose transport by targeting SGLT-1 and GLUT-2 transport proteins. We aimed to investigate whether homoeriodictyol, which is structurally related to hesperitin, affects glucose uptake in differentiated Caco-2 cells as a model for the intestinal barrier. The results revealed that, in contrast to other polyphenols, the flavanon homoeriodictyol promotes glucose uptake by 29.0 ± 3.83% at a concentration of 100 μM...
2017: PloS One
https://www.readbyqxmd.com/read/28190822/phloridzin-inhibits-high-k-induced-contraction-via-the-inhibition-of-sodium-glucose-cotransporter-1-in-rat-ileum
#4
Hidenori Kanda, Takeharu Kaneda, Akira Kawaguchi, Noriyasu Sasaki, Tsuyoshi Tajima, Norimoto Urakawa, Kazumasa Shimizu, Hiroetsu Suzuki
Recent studies have shown that phloridzin, an inhibitor of sodium-glucose cotransporter (SGLT), strongly decreases high K(+)-induced contraction in phasic muscle, such as tenia coli, but slightly affects tonic muscle, such as trachea . In this study, we examined the inhibitory mechanism of phloridzin on high K(+)-induced muscle contraction in rat ileum, a phasic muscle. Phloridzin inhibited the high K(+)-induced contraction in the ileum and the aorta, and the relaxing effect of phloridzin at 1 mM in the ileum was approximately five-fold more potent than that in the aorta...
February 11, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28081477/pharmacokinetic-studies-of-active-triterpenoid-saponins-and-the-total-secondary-saponin-from-anemone-raddeana-regel
#5
Dandan Zhang, Tianli Lei, Chongning Lv, Huimin Zhao, Haiyan Xu, Jincai Lu
The rhizome of Anemone raddeana Regel, a Traditional Chinese Medicine (TCM) which has a robust history treating rheumatism and neuralgia. The total secondary saponin (TSS) from it has demonstrated antitumor activity. In this study, a rapid and validated LC-MS/MS method was developed to simultaneously determine the active compounds (Hederacolchiside A1 and Eleutheroside K). Analytes were separated on a reverse-phase C18 column with acetonitrile-water (5mmol/L ammonium acetate) as the mobile phase. This assay showed acceptable linearity (r>0...
January 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28042856/effect-of-selected-plant-phenolics-on-fe-2-edta-h%C3%A2-o%C3%A2-system-mediated-deoxyribose-oxidation-molecular-structure-derived-relationships-of-anti-and-pro-oxidant-actions
#6
Jeffrey de Graft-Johnson, Dariusz Nowak
In the presence of transition metal ions and peroxides, polyphenols, well-known dietary antioxidants, can act as pro-oxidants. We investigated the effect of 13 polyphenols and their metabolites on oxidative degradation of deoxyribose by an (•)OH generating Fenton system (Fe(2+)-ethylenediaminetetraacetic acid (EDTA)-H₂O₂). The relationship between phenolics pro-oxidant/anti-oxidant effects and their molecular structure was analyzed using multivariate analysis with multiple linear regression and a backward stepwise technique...
December 31, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27973994/influence-of-static-magnetic-fields-up-to-700-mt-and-dihydrochalcones-on-the-antioxidant-response-in-fibroblasts
#7
Agnieszka Synowiec-Wojtarowicz, Magdalena Kimsa-Dudek, Katarzyna Pawłowska-Góral, Ewa Kurzeja, Marek Glinka, Stanisław Gawron
The effects of a static magnetic field (SMF) and the dihydrochalcones phloretin and phloridzin on the redox homeostasis of fibroblasts were investigated. The aim of the present study was to determine the redox homeostasis of fibroblasts that were simultaneously exposed to a static magnetic field and the dihydrochalcones phloretin and phloridzin. The fibroblasts were cultured for 72 h in special magnetic test chambers at different moderate intensities (0.4, 0.55 and 0.7 T). In this report, the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione transferase (GST); the concentrations of malondialdehyde (MDA), adenosine triphosphate (ATP) and total antioxidant status were measured using commercially available kits...
March 21, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/27894824/inhibitory-effect-of-phloretin-on-%C3%AE-glucosidase-kinetics-interaction-mechanism-and-molecular-docking
#8
Lin Han, Chun Fang, Ruixue Zhu, Qiang Peng, Ding Li, Min Wang
As the aglycone of phloridzin, phloretin belongs to dihydrochalcone with antioxidant, anti-inflammatory and antimicrobial activities. In this study, multispectroscopic techniques and molecular docking analysis were used to investigate the inhibitory activity and mechanisms of phloretin on α-glucosidase. The results showed that phloretin reversibly inhibited α-glucosidase in a mixed-type manner and the value of IC50 was 31.26μgL(-1). The intrinsic fluorescence of α-glucosidase was quenched by the interactions with phloretin through a static quenching mechanism and spontaneously formed phloretin-α-glucosidase complex by the driving forces of van der Waals force and hydrogen bond...
February 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27893760/leaf-extract-from-lithocarpus-polystachyus-rehd-promote-glycogen-synthesis-in-t2dm-mice
#9
Jinfei Wang, Yumin Huang, Kaixiang Li, Yingying Chen, Diana Vanegas, Eric Scott McLamore, Yingbai Shen
The purpose of this study was to investigate the effects of leaf extract from Lithocarpus polystachyus Rehd. on type II diabetes mellitus (T2DM) and the active ingredients of this effect. In addition, this study determined, for the first time, the underlying molecular and pharmacological mechanisms of the extracts on hyperglycemia using long-term double high diet-fed and streptozotocin (STZ) induced type II diabetic mice. In the present study, leaf extract, phloridzin and trilobatin were assessed in vivo (gavage) and in vitro (non-invasive micro-test technique, NMT) in experimental T2DM mice...
2016: PloS One
https://www.readbyqxmd.com/read/27883364/determination-of-s-methyl-l-methionine-smm-from-brassicaceae-family-vegetables-and-characterization-of-the-intestinal-transport-of-smm-by-caco-2-cells
#10
Ji-Hoon Song, Hae-Rim Lee, Soon-Mi Shim
The objectives of the current study were to determine S-methyl-L-methionine (SMM) from various Brassicaceae family vegetables by using validated analytical method and to characterize the intestinal transport mechanism of SMM by the Caco-2 cells. The SMM is well known to provide therapeutic activity in peptic ulcers. The amount of SMM from various Brassicaceae family vegetables ranged from 89.08 ± 1.68 μg/g to 535.98 ± 4.85 μg/g of dry weight by using validated ultra-performance liquid chromatography-electrospray ionization-mass spectrometry method...
January 2017: Journal of Food Science
https://www.readbyqxmd.com/read/27853059/dose-dependent-effect-of-glucose-on-glp-1-secretion-involves-sweet-taste-receptor-in-isolated-perfused-rat-ileum-rapid-communication
#11
Zhiwei Xu, Wendong Wang, Xiaofeng Nian, Guiqin Song, Xiaoyun Zhang, Hongyuan Xiao, Xiaobo Zhu
Luminal glucose is an important stimulus for glucagon-like peptide 1 (GLP-1) secretion from intestinal endocrine cells. However, the effects of luminal glucose concentration on GLP-1 secretion remain unknown. In this study, we investigated the effect of luminal glucose concentrations (3.5, 5, 10, 15, and 20 mmol/L) on GLP-1 secretion from isolated perfused rat ileum. Results showed that the perfusate glucose concentration dose-dependently stimulated GLP-1 secretion from isolated perfused rat ileum, which was eliminated by the sweet taste receptor inhibitor gurmarin (30 μg/mL), but not inhibited by phloridzin (1 mmol/L), a Na(+)-coupled glucose transporters inhibitor...
November 15, 2016: Endocrine Journal
https://www.readbyqxmd.com/read/27731384/purification-and-characterization-of-a-novel-phloretin-2-o-glycosyltransferase-favoring-phloridzin-biosynthesis
#12
Tingjing Zhang, Jianqiang Liang, Panxue Wang, Ying Xu, Yutang Wang, Xinyuan Wei, Mingtao Fan
Phloretin-2'-O-glycosyltransferase (P2'GT) catalyzes the last glycosylation step in the biosynthesis of phloridzin that contributes to the flavor, color and health benefits of apples and processed apple products. In this work, a novel P2'GT of Malus x domestica (MdP2'GT) with a specific activity of 46.82 μkat/Kg protein toward phloretin and uridine diphosphate glucose (UDPG) at an optimal temperature of 30 °C and pH 8.0 was purified from the engineered Pichia pastoris broth to homogeneity by anion exchange chromatography, His-Trap affinity chromatography and gel filtration...
October 12, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27688003/high-triterpenic-acids-production-in-callus-cultures-from-fruit-pulp-of-two-apple-varieties
#13
Giancarlo Verardo, Andrea Gorassini, Donata Ricci, Daniele Fraternale
INTRODUCTION: Very rarely fruit pulp has been used in in vitro culture to produce secondary metabolites useful in promoting health. OBJECTIVES: The aims of this work were the study of the best conditions to obtain the callus cultures from the pulp of two varieties of apples, Golden Delicious (GD) and "Mela Rosa Marchigiana" (MRM), and the quali-quantitative analysis of secondary metabolites produced by the two in vitro callus cultures. METHODOLOGY: Callus was induced on both Murashige and Skoog and Gamborg B5 media containing various combinations of supplements...
January 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27595398/expression-of-jak3-sensitive-na-coupled-glucose-carrier-sglt1-in-activated-cytotoxic-t-lymphocytes
#14
Shefalee K Bhavsar, Yogesh Singh, Piyush Sharma, Vishal Khairnar, Zohreh Hosseinzadeh, Shaqiu Zhang, Monica Palmada, Ivan Sabolic, Hermann Koepsell, Karl S Lang, Philipp A Lang, Florian Lang
BACKGROUND: Similar to tumor cells, activated T-lymphocytes generate ATP mainly by glycolytic degradation of glucose. Lymphocyte glucose uptake involves non-concentrative glucose carriers of the GLUT family. In contrast to GLUT isoforms, Na+-coupled glucose-carrier SGLT1 accumulates glucose against glucose gradients and is effective at low extracellular glucose concentrations. The present study explored expression and regulation of SGLT1 in activated murine splenic cytotoxic T cells (CTLs) and human Jurkat T cells...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27542458/experimental-and-molecular-docking-investigations-on-the-inclusion-mechanism-of-the-complex-of-phloridzin-and-hydroxypropyl-%C3%AE-cyclodextrin
#15
Chun-Ling Zhang, Jie-Chao Liu, Wen-Bo Yang, Da-Lei Chen, Zhong-Gao Jiao
Phloridzin is a nutraceutical. Its use in food, medicine and cosmetics is limited because of its low aqueous solubility and stability limits, but it can be improved by complexing with cyclodextrins. In this study, we investigated the inclusion mechanism between phloridzin and hydroxylpropyl-β-cyclodextrin (HP-β-CD) using isothermal titration calorimetry (ITC), ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), proton nuclear magnetic resonance spectroscopy ((1)H NMR) and molecular docking simulations...
January 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27535497/docosahexaenoic-acid-acylated-phloridzin-a-novel-polyphenol-fatty-acid-ester-derivative-is-cytotoxic-to-breast-cancer-cells
#16
Wasundara Fernando, Melanie R Power Coombs, David W Hoskin, H P Vasantha Rupasinghe
Docosahexaenoic acid-acylated phloridzin (PZ-DHA), a novel polyphenol fatty acid ester derivative, was synthesized through a regioselective acylation reaction with the aim of increasing the bioactivity of phloridzin (PZ) and docosahexaenoic acid (DHA). In this study, PZ-DHA's cytotoxic activity was explored using in vitro and in vivo models of mammary carcinoma. PZ-DHA was selectively cytotoxic for mammary carcinoma (MDA-MB-231, MDA-MB-468, 4T1, MCF-7 and T-47D) cells compared to non-malignant human mammary epithelial cells (HMEC and MCF-10A) and fibroblasts by MTS assay and Annexin-V-FLUOS/propidium iodide staining...
October 2016: Carcinogenesis
https://www.readbyqxmd.com/read/27520503/real-time-monitoring-of-glucose-and-phenols-intestinal-absorption-through-an-integrated-caco-2tc7cells-biosensors-telemetric-device-hypoglycemic-effect-of-fruit-phytochemicals
#17
Antonio Barberis, Antonella Garbetta, Angela Cardinali, Gianfranco Bazzu, Isabella D 'Antuono, Gaia Rocchitta, Angela Fadda, Vito Linsalata, Guy D 'Hallewin, Andrea Pier Serra, Fiorenza Minervini
An integrated device for real-time monitoring of glucose and phenols absorption, that consists of a sensors/biosensors system (SB) and a Caco-2TC7 human intestinal cell culture, is described in this study. The SB is composed of a glucose oxidase-based biosensor, a sentinel platinum sensor, a laccase/tyrosinase-based biosensor and a sentinel carbon sensor, all located in the basolateral compartment (BC) of a cell culture plate. Caco-2TC7 cells, differentiated on culture inserts, separated the apical compartment that simulates the intestinal lumen, from the BC which represented the bloodstream...
February 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27486478/screening-for-antioxidant-and-antibacterial-activities-of-phenolics-from-golden-delicious-apple-pomace
#18
Tingjing Zhang, Xinyuan Wei, Zhuang Miao, Hamada Hassan, Yunbo Song, Mingtao Fan
BACKGROUND: Synthetic antioxidants and antimicrobials are losing ground to their natural counterparts and therefore, the food industry has motivated to seek other natural alternatives. Apple pomace, a by-product in the processing of apples, is rich in polyphenols, and plant polyphenols have been used as food additives owing to their strong antioxidant and antimicrobial properties. The goal of this study was to screen the individual polyphenols with antioxidant and antimicrobial activities from the extracts (methanol, ethanol, acetone, ethyl acetate, and chloroform) of Golden Delicious pomace...
2016: Chemistry Central Journal
https://www.readbyqxmd.com/read/27441252/chemical-composition-and-antioxidant-prooxidant-potential-of-a-polyphenolic-extract-and-a-proanthocyanidin-rich-fraction-of-apple-skin
#19
Ana María Mendoza-Wilson, Sergio Ivan Castro-Arredondo, Angélica Espinosa-Plascencia, María Del Refugio Robles-Burgueño, René Renato Balandrán-Quintana, María Del Carmen Bermúdez-Almada
The apple is a food rich in diverse classes of polyphenols (PP), among which the proanthocyanidins (PCs), which are primarily concentrated in the skin, are one of the most abundant. These compounds are of considerable interest for their possible positive health effects because of their antioxidant properties. However, depending on the classes of PP present (chemical composition) and their relative concentrations in the apple skin, their antioxidant effects vary and some of their components can even generate prooxidant effects...
February 2016: Heliyon
https://www.readbyqxmd.com/read/27283697/apple-phenolics-as-inhibitors-of-the-carbonylation-pathway-during-in-vitro-metal-catalyzed-oxidation-of-myofibrillar-proteins
#20
Tine Rysman, Mariana Utrera, David Morcuende, Geert Van Royen, Stephanie Van Weyenberg, Stefaan De Smet, Mario Estévez
The effect of apple phenolics on the oxidative damage caused to myofibrillar proteins by an in vitro metal-catalyzed oxidation system was investigated. Three pure phenolic compounds (chlorogenic acid, (-)-epicatechin and phloridzin) and an apple peel extract were added to myofibrillar proteins in three concentrations (50, 100 and 200μM), and a blank treatment was included as a control. All suspensions were subjected to Fe(3+)/H2O2 oxidation at 37°C during 10days, and protein oxidation was evaluated as carbonylation (α-amino adipic and γ-glutamic semialdehydes) and Schiff base cross-links...
November 15, 2016: Food Chemistry
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