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Cervical intraepithelial lesions

Nehal M Fahim, Iman H Shehata, Shereen E Taha, Ragia A Fahmy, Manar S Elsayed
Human leukocyte antigen-G (HLA-G) is a non-classical HLA-class Ib molecule with multiple immunoregulatory properties. Its main functions in physiological conditions are to abolish maternal immune cell activity against fetus and to establish immune tolerance at the maternal-fetal interface. Cervical tumor cells have been reported to express HLA-G which is one of the immunomodulatory molecules that is involved in every phase of cancer immunoediting. It has inhibitory functions against natural killer (NK) cells, T-lymphocytes, and antigen-presenting cells (APCs)...
January 2018: Egyptian Journal of Immunology
Usha Rani Poli, Swarnalata Gowrishankar, Meenakshi Swain, Jose Jeronimo
PURPOSE: Human papillomavirus (HPV) DNA screening reduces cervical cancer incidence and mortality in low-resource settings. Self-collected vaginal samples tested with affordable HPV tests such as careHPV can increase the rate of screening in resource-constrained settings. We report the role of visual inspection with acetic acid (VIA) as a triage test for women testing positive with the careHPV test on self-collected vaginal samples. METHODS: As part of a multicountry demonstration study, 5,207 women 30 to 49 years of age were recruited from urban slums to undergo four cervical screening tests using the careHPV test on self-collected vaginal samples, provider-collected cervical samples, the Papanicolaou test, and VIA...
September 2018: Journal of Global Oncology
Ana Gradíssimo, Jessica Lam, John D Attonito, Joel Palefsky, L Stewart Massad, Xianhong Xie, Isam-Eldin Eltoum, Lisa Rahangdale, Margaret A Fischl, Kathryn Anastos, Howard Minkoff, Xiaonan Xue, Gypsyamber D'Souza, Lisa C Flowers, Christine Colie, Sadeep Shrestha, Nancy A Hessol, Howard D Strickler, Robert D Burk
BACKGROUND: HIV-positive women are at substantial risk of HPV-associated cervical neoplasia caused by high-risk (HR) HPVs. Methylation of the HPV genome is associated with cervical intraepithelial neoplasia grade 3 (CIN3) in HIV-negative women, yet it is unknown whether this holds true for HIV-positive women. METHODS: We designed a case-control study within the WIHS cohort comparing HIV-positive CIN3 cases (N=72) to HIV-positive controls without detectable CIN2+...
September 20, 2018: Cancer Epidemiology, Biomarkers & Prevention
Omna Shaki, Barun Kumar Chakrabarty, N Nagaraja
Introduction: Cervical cancer is the most common cause of death among women in developing countries. Among the Indian women, cervical cancer is the most common genital tract cancer. Papanicolaou (Pap) smear test plays a vital role in the detection of cervical cancer even in its premalignant condition. The aim of this study to evaluate the role of Pap smear in detecting premalignant and malignant lesions as well as nonneoplastic lesions of the cervix and to determine the prevalence of various lesions...
July 2018: Journal of Family Medicine and Primary Care
Guanyu Ruan, Yiyi Song, Binhua Dong, Xiaodan Mao, Fen Lin, Yafang Kang, Shuxia Xu, Xianjing Chen, Qibin Wu, Pengming Sun
Objectives: The Cervista® high-risk human papillomavirus (HR-HPV) test was evaluated as a primary screening method for cervical cancer in women aged ≥21 years and was compared with different screening and triage combinations. Materials and methods: A nested case-control study within the Fujian provincial Cervical Lesion Screening Cohorts was used to evaluate the Cervista test as the primary cervical screening method in a hospital-based population. Strategy 1 primarily screened using a cytology screen with HR-HPV testing used for triage...
2018: Cancer Management and Research
Stephanie S Liu, Karen Kl Chan, Daniel Kh Chu, Tina N Wei, Lesley Sk Lau, Siew F Ngu, Mandy My Chu, Ka Yu Tse, Philip Pc Ip, Enders Ko Ng, Annie Ny Cheung, Hextan Ys Ngan
Cervical cancer is one of the leading causes of cancer death in women globally, despite widespread use of cytology/Human Papillomavirus (HPV) screening. Here, we aimed to identify the potential role of miRNA as diagnostic biomarker in the detection of cervical pre-malignant lesions and cancer. In total, we recruited 582 patients with cervical diseases and 145 control individuals. The expression levels of six miRNAs (miR-20a, miR-92a, miR-141, miR-183*, miR-210 and miR-944) were found to be significantly up-regulated in cervical cancer and pre-malignant lesions as compared to normal cervical samples, indicating that they are oncogenic miRNAs...
September 17, 2018: Molecular Oncology
Annemiek Leeman, Renée M F Ebisch, Annemieke Kasius, Remko P Bosgraaf, David Jenkins, Miekel M van de Sandt, Lise M A de Strooper, Daniëlle A M Heideman, Peter J F Snijders, Leon F A G Massuger, Ruud L M Bekkers, Chris J L M Meijer, Folkert J van Kemenade, Wim G V Quint, Willem J G Melchers
OBJECTIVE: HPV16/18 genotyping and detection of hypermethylation of human cell genes involved in cervical oncogenesis have shown promising results in triage of high-risk HPV (hrHPV)-screen positive women on cervical smears. These tests can be performed on self-samples, which contain cervical and vaginal cells. We studied whether a self-sample represents the hrHPV type causing the worst cervical lesion and whether any differences in hypermethylation of FAM19A4/miR124-2 exist between CIN lesions caused by different hrHPV types...
September 12, 2018: Gynecologic Oncology
Urska Ivanus, Tine Jerman, Alenka Repse Fokter, Iztok Takac, Veronika Kloboves Prevodnik, Mateja Marcec, Ursula Salobir Gajsek, Maja Pakiz, Jakob Koren, Simona Hutter Celik, Kristina Gornik Kramberger, Ulrika Klopcic, Rajko Kavalar, Simona Sramek Zatler, Biljana Grcar Kuzmanov, Mojca Florjancic, Natasa Nolde, Srdjan Novakovic, Mario Poljak, Maja Primic Zakelj
Background To overcome obstacles within the Slovenian organised cervical cancer screening programme, a randomised pilot study of human papillomavirus (HPV) self-sampling among non-attenders was performed, aiming to assess three different screening approaches. Participants and methods Non-attenders aged 30-64 years from two Slovenian regions were randomised to two HPV self-sampling groups-the opt-in (I1, n = 14.400) and the opt-out (I2, n = 9.556), with a control group (P, n = 2.600). Self-collected samples were analysed using the Hybrid Capture 2 assay...
September 14, 2018: Radiology and Oncology
Naouel Tifaoui, Thierry Maudelonde, Jacques Combecal, Roselyne Vallo, Sylviane Doutre, Marie-Noëlle Didelot, Nicolas Nagot, Michel Segondy, Nathalie Boulle
BACKGROUND: With population ageing, post-menopausal women represent a new group to be considered in cervical cancer screening strategies, including the significance of High Risk (HR)-HPV detection. OBJECTIVES: A retrospective analysis was conducted in a cohort of 406 menopausal women attending routine gynaecological consultation at the Hospital of Montpellier (France). STUDY DESIGN: All women benefited from a cervical smear and HR-HPV detection using Hybrid Capture 2 (HC2) test...
August 31, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Yunpeng Ji, Xiao-Xia Ma, Zhaocai Li, Maikel P Peppelenbosch, Zhongren Ma, Qiuwei Pan
Background: Chlamydia trachomatis may coinfect with human papillomavirus (HPV) and complicate the cervical pathogenesis. This study aimed to evaluate the prevalence, risk factors, and clinical outcomes of HPV/C. trachomatis coinfection in women from Inner Mongolia, China. Methods: We performed a polymerase chain reaction (PCR)-based HPV/C. trachomatis screening and cervical samples were analyzed by thinprep cytologic test. Statistical analysis was used to assess the association between demographic factors and coinfection...
September 5, 2018: Journal of Infectious Diseases
Fangda Leng, Lu Jiang, Lin Nong, Xiying Ren, Tong Xie, Ying Dong, Xia Tao
AIM: To investigate the features of skip lesions and evaluate value of top-hat procedure in management of squamous intraepithelial lesion. METHODS: We reviewed the records of patients who underwent loop electrosurgical excision procedure (LEEP) in Peking University First Hospital between 2011 and 2016. Patients were confirmed to have CIN1-3. The term 'skip lesion' refers to lesion lying deep in cervical canal discontiguous with other lesions in transformation zone and was confirmed by top-hat...
September 6, 2018: Journal of Obstetrics and Gynaecology Research
Sheila E Segura, Gloria Ramos-Rivera, Laleh Hakima, Mark Suhrland, Samer Khader
BACKGROUND: The 2014 Bethesda System for Reporting Cervical Cytology classifies squamous intraepithelial lesions (SILs) of cervix in 2 main categories: Low-grade SIL (LSIL) and High-grade SIL (HSIL). In some clinical practices, "LSIL cannot rule out high grade lesion (LROH)" interpretive category is used in cases with LSIL and findings that may raise the possibility of HSIL. Our purpose is to assess follow-up histopathology and high-risk human papillomavirus (hrHPV) results in patients with LROH, in comparison with LSIL, atypical squamous cells, cannot rule out HSIL (ASC-H), and HSIL in our institution...
September 6, 2018: Cytopathology: Official Journal of the British Society for Clinical Cytology
Erdem Sahin, Yusuf Madendag, Mefkure Eraslan Sahin, Ilknur Col Madendag, Gokhan Acmaz, Cıgdem Karakukcu, Hatice Karaman, Iptisam Ipek Muderris
PURPOSE: To evaluate cervical mucus secretory leukocyte protease inhibitor (SLPI) concentrations in patients with high-risk human papillomavirus (hrHPV) 16 or 18 positive and low-grade squamous intraepithelial lesions (LGSIL) or high-grade squamous intraepithelial lesions (HGSIL). METHOD: Patients with HPV 16 or 18 positive from 30 to 45 years of age whose cervical cancer screening results reported cytologically LGSIL or HGSIL were included in the study. In the control group, we included participants in the same age with cytology negative and HPV-negative healthy women...
January 2018: Cancer Control: Journal of the Moffitt Cancer Center
Valentina Fiano, Morena Trevisan, Francesca Fasanelli, Chiara Grasso, Federica Marabese, Maria da Graça Bicalho, Newton S de Carvalho, Carlos A Maestri, Franco Merletti, Carlotta Sacerdote, Laura De Marco, Anna Gillio-Tos
OBJECTIVE: The present study aimed to evaluate the association between altered methylation and histologically confirmed high grade cervical intraepithelial neoplasia (hgCIN). METHODS: Methylation levels in selected host (CADM1, MAL, DAPK1) and HPV (L1_I, L1_II, L2) genes were measured by pyrosequencing in DNA samples obtained from 543 women recruited in Curitiba (Brazil), 249 with hgCIN and 294 without cervical lesions. Association of methylation status with hgCIN was estimated by Odds Ratio (OR) with 95% confidence interval (CI)...
August 29, 2018: Gynecologic Oncology
Sebastián Videla, Antoni Tarrats, Arelly Ornelas, Roger Badia, Eva Castella, Carme Alcalde, Anna Chamorro, José A Esté, Bonaventura Clotet, Guillem Sirera
Currently, Papanicolaou smears are proposed at three-year intervals for cervical screening to all women living with HIV. The aim of this retrospective cohort study was to provide data on the incidence of cervical high-grade squamous intraepithelial lesions (HSIL) in cervical smear confirmed by histology in HIV-1-infected women (two consecutive normal Papanicolaou smears at baseline) after a long-term follow-up. Sixty-seven women (recruited between March 1999 and January 2003) were analyzed. The median period of follow-up was 13...
August 31, 2018: International Journal of STD & AIDS
Kleber Paiva Trugilo, Guilherme Cesar Martelossi Cebinelli, Fernanda Costa Brandão Berti, Nádia Calvo Martins Okuyama, Fernando Cezar-Dos-Santos, Michelle Mota Sena, Luis Fernando Lásaro Mangieri, Maria Angelica Ehara Watanabe, Karen Brajão de Oliveira
The main purpose was to assess the effect of c.29C>T and c.74G>C polymorphisms in the TGFB1 signal peptide on HPV infection and development of cervical lesions. Cervical swabs and blood samples were obtained from 349 outpatient women, along with socio-demographic and sexual behavioral data. The study population was stratified by absence or presence of HPV DNA, as tested by PCR, as well as by lesion grade. TGFB1 signal peptide polymorphisms were genotyped using PCR-restriction fragment length polymorphism...
August 30, 2018: Medical Microbiology and Immunology
Lin Jing, Wu Dan, Li Zhunan, Xu Ying, Chen Yi
OBJECTIVE: To identify the risk factors for residual lesion in hysterectomy specimens after loop electrosurgical excision procedure (LEEP) for cervical intraepithelial neoplasia (CIN). METHODS AND RESULTS: We retrospectively analyzed the clinical data of 594 patients who underwent total hysterectomy after LEEP for CIN at the International Peace Maternity and Child Health Hospital affiliated to Shanghai Jiaotong University between July 2006 and June 2015. Among the 594 patients, there were no residual lesions in uterine specimens of 409 (68...
October 2018: Archives of Gynecology and Obstetrics
Shikha Suman, Ashutosh Mishra
Overlapping genes across high-grade squamous intraepithelial lesions (CIN2 and 3) and cancer may serve as potential biomarkers for this progressive disease. Differentially expressed genes (DEGs) of dysplastic (CIN2 and CIN3) and cancer cells were identified by microarray data analysis. Gene interaction network was constructed using the 98 common DEGs among the dysplastic and cancer cells and analysed for the identification of common modules, hubs and significant motifs. Two significant modules and 10 hubs of the common gene interaction network, with 125 nodes and 201 edges were found...
August 27, 2018: Scientific Reports
Min-Sun Jin, Hyebin Lee, Min A Kim, In Ae Park, Chul Lee, Hyoung Jin An, Bobae Shim, Ji Hye Moon, Jae Kyung Won, Han Suk Ryu
BACKGROUND: Although the Papanicolaou (Pap) test is the first-line screening method for cervical cancer, it has low sensitivity for detection of human papillomavirus (HPV)-infected cervical lesion compared to the HPV test. The aims of this study are to determine novel cytomorphologic parameters for HPV infection in patients previously diagnosed as negative for intraepithelial lesion or malignancy (NILM) and to comparatively analyze the detection performance of 3 HPV tests: nested PCR, the DNA Chip test, and the Liquid Beads Microarray (LBMA) assay...
August 25, 2018: Diagnostic Cytopathology
Yuping Dai, Lirong Wang, Donghong Li
Folate receptor-mediated staining solution detection (FRD) has been recently suggested as an effective tool in the cervical cancer screening. We aim to compare the accuracy of FRD to human papillomavirus (HPV) and thinprep cytology test (TCT) based on cytology-based biopsy.During May 2016 and December 2016, we recruited women who presented for routine cervical lesion screening. The eligible cases were nonpregnant women and not in the menstrual period, aging between 25 and 65. All eligible women were screened with TCT, HPV, FRD testing, and colposcopy...
August 2018: Medicine (Baltimore)
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