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Cervical intraepithelial lesions

Mónica Molano, Oscar Buitrago, Pablo Moreno-Acosta, Suzanne M Garland, Nicolás Morales, Antonio Huertas, Teresa Martinez, Oscar Gamboa, Sepehr N Tabrizi, Alyssa Cornall, Nubia Muñoz
Certain variants of human papillomavirus (HPV)type 58 are associated with an increased risk of high grade squamous intraepithelial lesions and cervical cancer. However, little is known about the persistence of HPV58 E6/E7 variants in women with incident HPV58 infections. The aim of the present study was to evaluate the presence and persistence of HPV58 E6/E7 variants in 71 women with incident HPV58 infection throughout their follow-up. These women belonged to a cohort examined in a longitudinal study of 1,610 Colombian women, who were HPV-negative and had normal baseline cytology...
August 2018: Oncology Letters
Marjolein van Zummeren, Annemiek Leeman, Wieke W Kremer, Maaike C G Bleeker, David Jenkins, Miekel van de Sandt, Daniëlle A M Heideman, Renske Steenbergen, Peter J F Snijders, Wim G V Quint, Johannes Berkhof, Chris J L M Meijer
AIMS: To investigate the accuracy and reproducibility of a scoring system for cervical intraepithelial neoplasia (CIN1-3) based on immunohistochemical (IHC) biomarkers Ki-67 and p16ink4a . METHODS: 115 cervical tissue specimens were reviewed by three expert gynaecopathologists and graded according to three strategies: (1) CIN grade based on H&E staining only; (2) immunoscore based on the cumulative score of Ki-67 and p16ink4a only (0-6); and (3) CIN grade based on H&E supported by non-objectified IHC 2 weeks after scoring 1 and 2...
July 16, 2018: Journal of Clinical Pathology
Ann Edblad-Svensson, Lena Silfverdal, Pia Collberg, Katarina Tunón
OBJECTIVE: To determine whether high-risk types of human papilloma virus (hrHPV) DNA testing is reliable for selection patients in need of further investigation with colposcopy in women with increased risk of high-grade cervical lesions as a result of false negative cytology. The secondary objective was to compare the sensitivity of hrHPV testing on self-collected versus physician-collected samples for the detection of histological high-grade cervical intraepithelial neoplasia (CIN2+)...
July 13, 2018: Acta Cytologica
C J Chibwesha, B Goeieman, S Levin, M Mulongo, M Faesen, A Swarts, S Ramotshela, S Williams, N Rakhombe, S Bruce, P Michelow, C Firnhaber
BACKGROUND: Cervical cancer remains the second most common cancer among women worldwide, with much of the global burden occurring in low- and middle-income countries. HIV-infected women are at increased risk of human papillomavirus infection, preinvasive cervical disease and invasive cervical cancer (ICC). Funded through the United States President's Emergency Plan for AIDS Relief (PEPFAR) and working in collaboration with the South African (SA) Department of Health, our team supports cervical screening integrated within public sector HIV clinics in SA...
February 27, 2018: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
Giselle Fachetti-Machado, Rosane Ribeiro Figueiredo-Alves, Marise Amaral Rebouças Moreira
OBJECTIVE:  To estimate the cytological and colposcopic performances for the diagnosis of cervical neoplasias. METHODS:  Cross-sectional retrospective study with data from patients' charts. The participants underwent colposcopy, guided biopsies, and excision when needed. The cytological and colposcopic categorization followed the Bethesda System and the international colposcopic terminologies. The cytology and colposcopy performances were evaluated by sensitivity (SE), specificity (SP), positive predictive value (PPV), and negative predictive value (NPV) analyses with 95% confidence interval (95% CI)...
July 11, 2018: Revista Brasileira de Ginecologia e Obstetrícia
Suzanne M Garland, Elmar A Joura, Kevin A Ault, F Xavier Bosch, Darron R Brown, Xavier Castellsagué, Alex Ferenczy, Daron G Ferris, Anna R Giuliano, Mauricio Hernandez-Avila, Warner K Huh, Ole-Erik Iversen, Susanne K Kjaer, Robert J Kurman, Joaquin Luna, Joseph Monsonego, Nubia Muñoz, Jorma Paavonen, Punnee Pitisuttihum, Brigitte M Ronnett, Marc Steben, Mark H Stoler, Cosette M Wheeler, Dorothy J Wiley, Gonzalo Perez, Alfred J Saah, Alain Luxembourg, Se Li, Mark J DiNubile, Monika Wagner, Christine Velicer
OBJECTIVE: To estimate the proportion of vulvar and vaginal low-grade and high-grade squamous intraepithelial lesions (LSILs and HSILs) in females 15-26 years of age attributable to 14 human papillomavirus (HPV) genotypes (6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59). METHODS: A post hoc analysis of prospectively diagnosed vulvar and vaginal LSILs and HSILs among females 15-26 years of age enrolled in the placebo arms of two phase 3, randomized HPV vaccine trials assessed 14 prespecified HPV genotypes associated with cervical cancers or anogenital warts using a type-specific multiplex polymerase chain reaction assay...
July 10, 2018: Obstetrics and Gynecology
Chelsey C Spriggs, Luis Z Blanco, Kruti P Maniar, Laimonis A Laimins
Human papillomaviruses (HPVs) are DNA viruses with epithelial tropism. High-risk types of HPV are the causative agents of the majority of cervical cancers and are responsible for a number of other anogenital as well as oropharyngeal cancers. The life cycle of HPV is closely linked to the differentiation state of its host cell and is dependent on the activation of specific pathways of the DNA damage response. Several proteins from the ataxia telangiectasia mutated and the ataxia telangiectasia mutated and Rad3-related DNA repair pathways, which are essential for maintaining genomic stability in cells, are upregulated in HPV-positive cells and are required for viral replication...
July 10, 2018: International Journal of Gynecological Pathology
Hiroaki Komatsu, Tetsuro Oishi, Daiken Osaku, Mayumi Sawada, Akiko Kudoh, Michiko Nonaka, Jun Chikumi, Shinya Sato, Tasuku Harada
OBJECTIVES: The aim of this study was to evaluate the diagnostic significance of high-risk human papillomavirus (hrHPV) testing for managing women with atypical glandular cells (AGC) and to explore the distribution of hrHPV genotypes. METHODS: We analyzed cytologic and histopathologic diagnoses in patients referred to our institution due to AGC or atypical squamous cells of undetermined significance (ASC-US). All patients underwent hrHPV testing and genotyping, and positive (PPV) and negative predictive values (NPV) for cervical intraepithelial neoplasia (CIN) 2 or worse [CIN2+/adenocarcinoma in situ (AIS)+] were calculated...
July 10, 2018: Acta Cytologica
Lichen Guo, Aimin Zhao, Rui Zhang, Yan Xie, Xiaofeng Xu
AIMS: To discuss the correlation between the expression of fibroblast growth factor receptor 4 (FGFR4) and human papillomavirus (HPV) infection in cervical cancer and precancerous lesions. METHODS: One hundred and sixty four cases of cervical lesions tissue samples were collected (60 cases of cervical cancer, 38 cases of cervical intraepithelial neoplasia [CIN] stage I, 32 cases of CIN II, 34 cases of CIN III). HPV infection status and expression strength of FGFR4 of all samples were detected and compared among different groups...
July 6, 2018: Gynecologic and Obstetric Investigation
Huilan Xu, Sam Egger, Louiza S Velentzis, Dianne L O'Connell, Emily Banks, Jessica Darlington-Brown, Karen Canfell, Freddy Sitas
BACKGROUND: Human papillomavirus (HPV) vaccines protect against HPV types 16/18, but do not eliminate the need to detect pre-cancerous lesions. Australian women vaccinated as teenage girls are now entering their mid-thirties. Since other oncogenic HPV types have been shown to be more prevalent in women ≥30 years old, understanding high grade cervical lesions in older women is still important. Hormonal contraceptives (HC) and smoking are recognised cofactors for the development of pre-malignant lesions...
July 3, 2018: Cancer Epidemiology
Joseph D Coppock, Brian C Willis, Mark H Stoler, Anne M Mills
BACKGROUND: In situ hybridization for human papillomavirus (HPV) messenger RNA (HPV RNA ISH) recently was introduced as an ancillary tool in the diagnosis of cervical squamous intraepithelial lesions, and can aid in the distinction between low-grade squamous intraepithelial lesions (LSILs) versus reactive/negative biopsies. Prior work has shown that up to one-half of cases originally diagnosed as LSIL are reclassified as negative/reactive by expert consensus review of morphology, and negative HPV RNA ISH results most often correlate with an expert diagnosis of negative/reactive...
July 5, 2018: Cancer Cytopathology
Zhengchen Guo, Yuanjing Hu, Liqin Yuan, Na Li, Tian Wang
PURPOSE: To study the predictive value of the DNA methylation levels of JAM3, SOX1, SLIT2, C13ORF18, and TERT in the cervical intraepithelial neoplasia prognosis. METHOD: In the present study, 139 cases were collected and followed up for 24 months. The DNA methylation levels of JAM3, SOX1, SLIT2, C13ORF18, and TERT were tested from their exfoliated cells. One-way ANOVA, receiver operating characteristic (ROC) curve analyses were conducted to analyze the data. RESULTS: The DNA methylation of the five genes was associated with prognosis of CIN...
July 3, 2018: Archives of Gynecology and Obstetrics
Lu Li, Wen-Ting Yang, Peng-Sheng Zheng, Xiao-Fang Liu
The SRY-box containing gene 17 (SOX17) is considered as a regulator in stemness maintenance and a suppressor in some malignant tumors. However, the biological function and molecular mechanism of SOX17 in the process of initiation and progression of cervical cancer remain obscure. In this study, immunohistochemistry showed that the expression of SOX17 was high in the normal cervix, moderate in the high-grade squamous intraepithelial lesion, and low in the cervical cancer. SOX17 inhibited the proliferation and viability of cervical cancer cells in vitro as well as tumor formation in vivo...
July 3, 2018: Cell Death & Disease
Gerard J Nuovo, Cecilia Vianna de Andrade, Susanne I Wells, Marion Brusadelli, Alcina F Nicol
Acute human papillomavirus (HPV) infection of the cervix (cervical intraepithelial neoplasia, CIN) is marked by high copy episomal viral DNA and L1/L2 capsid protein expression (productive infection) in the cells towards the surface that facilitate sexual viral transmission. Viral DNA is low copy and not associated with viral capsid protein expression in the less differentiated lower part of the CIN (nonproductive infection). The purpose of this study was to examine the host response in these two areas. Serial section and co-localization analyses demonstrated that in 29/33 (88%) of cases the NF-κB pathway was activated and localized to the suprabasal nonproductively infected cells in the CIN lesions...
June 22, 2018: Annals of Diagnostic Pathology
Pushp Lata Sachan, Meenakshi Singh, Munna Lal Patel, Rekha Sachan
Objective: The objective of the study is to evaluate the use of the Pap smear screening method for detection of precancerous lesions. Methods: All women who visited the outpatient gynecology clinic of the Department of Obstetrics and Gynaecology at King Georges Medical University, Lucknow, UP, India, over 1 year for different clinical problems were recruited for the study. A total of 1650 women who were sexually active and over 21 years of age were enrolled in the study...
July 2018: Asia-Pacific Journal of Oncology Nursing
Caroline Domingues Rogeri, Henrique César Santejo Silveira, Rhafaela Lima Causin, Luisa Lina Villa, Maíra Degiovani Stein, Ana Carolina de Carvalho, Lídia Maria Rebolho Batista Arantes, Cristovam Scapulatempo-Neto, Júlio César Possati-Resende, Márcio Antoniazzi, Adhemar Longatto-Filho, José Humberto Tavares Guerreiro Fregnani
OBJECTIVES: The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. METHODS: A total of 447 cervical cytology samples obtained from women who underwent colposcopy were examined. The cases were distributed as follows: (1) cervices without cervical intraepithelial neoplasia (CIN; n = 152); (2) cervices with a CIN grade of 1 (CIN 1; n = 147); and (3) cervices with a CIN grade of 2 or 3 (CIN 2/3; n = 148)...
June 27, 2018: Gynecologic Oncology
Meritxell Munmany, Aureli Torné, Roser Nonell, Esther Barnadas, Nerea Luqui, Jaume Ordi, Marta Del Pino
OBJECTIVE: The aim of the study was to assess the accuracy of colposcopy evaluation at the time of the loop electrosurgical excision procedure (LEEP) to identify women with a previous confirmatory diagnosis of squamous intraepithelial lesion/cervical intraepithelial neoplasia (SIL/CIN) with low probability of dysplasia in the LEEP specimen. MATERIALS AND METHODS: We prospectively recruited a cohort of 162 women undergoing LEEP for histological high-grade SIL/CIN 2-3 or low-grade SIL/CIN 1 with high-grade SIL cytology showing a fully visible squamocolumnar junction in the colposcopy evaluation at the time of LEEP...
June 27, 2018: Journal of Lower Genital Tract Disease
Alia Albawardi, M Ruhul Quddus, Shamsa Al Awar, Saeeda Almarzooqi
BACKGROUND: The incidence of abnormal cervical smears in the United Arab Emirates (UAE) is 3.6%. Data regarding specific high-risk HPV (hrHPV) genotypes are insufficient. Identification of hrHPV subtypes is essential to allow formulating effective vaccination strategies. METHODS: A total of 75 archival cervical cone biopsies with HSIL or higher lesions (2012-2016) were retrieved from a tertiary hospital, including HSIL (n = 70), adenocarcinoma in-situ (n = 1) and squamous cell carcinoma (n = 4)...
June 26, 2018: Diagnostic Pathology
T Y Li, Z N Wu, M Y Jiang, J F Cui, B Liu, F Chen, W Chen
Objective: To explore the relationship between high risk HPV (HR-HPV) DNA load and cervical lesions in HR-HPV single/ multiple infections. Methods: Two thousand six hundred and forty-six women from Shanxi, Henan and Xinjiang were recruited into a cervical cancer screening program. Cervical exfoliated cell specimens collected from all of the participants were detected by hybrid capture Ⅱ (HC2), cytological diagnosis was performed according to the Bethesda System, and pathological diagnosis was interpreted using cervical intraepithelial neoplasia (CIN) terminology...
June 23, 2018: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
Sze Chuen Cesar Wong, Thomas Chi Chuen Au, Sammy Chung Sum Chan, Lawrence Po Wah Ng, Hin Fung Tsang
Background: Human papillomavirus (HPV) is a known causative factor in the etiology of cervical cancer. Methods: HPV DNA genotyping was performed in menstrual blood (MB) collected in napkins from patients with cervical intraepithelial neoplasia (CIN), HPV infection and sexually active apparently normal subjects. In the same patient cohort, MB TAP1 I333V and TAP1 D637G gene polymorphisms were examined. Results: The sensitivity, specificity, positive and negative predictive values of HPV DNA to detect CIN or HPV infection was 83% (223/268), 98% (131/134), 99% (223/226) and (74%) 131/176, respectively...
June 19, 2018: Journal of Infectious Diseases
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