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Cervical intraepithelial lesions

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https://www.readbyqxmd.com/read/29456720/mir-494-inhibits-cervical-cancer-cell-proliferation-through-upregulation-of-socs6-expression
#1
Lei Cheng, Beihua Kong, Ying Zhao, Jie Jiang
It is unclear how microRNA (miR)-494 inhibits the proliferation of cervical cancer cells by altering the expression of SOCS6. Therefore, the present study aimed to investigate the molecular mechanism underlying miR-494 regulation of suppressor of cytokine signaling 6 (SOCS6) in human cervical cancer samples and the human cervical cancer HeLa cell line. The expression of miR-494 was determined using reverse transcription-quantitative polymerase chain reaction. In addition, TargetScan was used to predict miR-494 target genes and the luciferase reporter assay was used to determine whether SOCS6 was a direct target of miR-494...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29447912/immunogold-agglutination-assay-for-direct-detection-of-hpv-16-e6-and-l1-proteins-from-clinical-specimens
#2
Parvapan Bhattarakosol, Kamolwan Plaignam, Amornpun Sereemaspun
HPV-16 infection is the most common cause of cervical cancer. As HPV-16 transforms the cell, E6 oncoprotein is over-expressed. Therefore, molecular detection of HPV-16 E6 mRNA is now being used for diagnosis and prediction of cancer development. Besides detecting E6 mRNA, a rapid lateral flow detecting the E6 protein using enzyme immunoassay is also now on market with a sensitivity of 53.5% for cervical intraepithelial neoplasia (CIN)-3 or more severe (CIN-3 + ). Here, an immunogold-agglutination assay was developed to detect not only HPV-16 E6 protein but also L1, a major capsid protein found in the productive stage of the virus...
February 12, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29446041/characteristic-findings-of-high-grade-cervical-intraepithelial-neoplasia-or-more-on-magnifying-endoscopy-with-narrow-band-imaging
#3
Kunihisa Uchita, Kenji Kanenishi, Koki Hirano, Hideki Kobara, Noriko Nishiyama, Ai Kawada, Shintaro Fujihara, Emi Ibuki, Reiji Haba, Yohei Takahashi, Yuka Kai, Kenji Yorita, Hirohito Mori, Jun Kunikata, Naoki Nishimoto, Toshiyuki Hata, Tsutomu Masaki
BACKGROUND: Colposcopy, which is a standard modality for diagnosing cervical intraepithelial neoplasia (CIN), can have limited accuracy owing to poor visibility. Flexible magnifying endoscopy with narrow band imaging (ME-NBI) has excellent diagnostic accuracy for early gastrointestinal neoplasms and is expected to be highly useful for CIN diagnosis. This study aimed to determine the characteristic findings and evaluate the diagnostic ability of ME-NBI for lesions ≥ CIN 3. METHODS: A well-designed prospective diagnostic case series conducted at multiple tertiary-care centers...
February 14, 2018: International Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29442221/dual-stained-cervical-cytology-and-histology-with-claudin-1-and-ki67
#4
Tímea Szekerczés, Ádám Galamb, Adrienn Kocsis, Márta Benczik, Tibor Takács, Attila Martonos, Balázs Járay, András Kiss, Csaba Jeney, Miklós Nyíri, Zsuzsa Schaff, Gábor Sobel
Several biomarkers are in use to improve the sensitivity and specificity of cervical cancer screening. Previously, increased expression of tight junction protein claudin-1 (CLDN1) was detected in premalignant and malignant cervical lesions and applied for cytology screening. To improve the specificity, a double immunoreaction with CLDN1/Ki67 was developed in the recent study. Parallel p16/Ki67 (CINtec® PLUS) and CLDN1/Ki67 dual-stained cytology and histology were performed and compared. p16/Ki67 immunoreaction showed positivity in 317 out of 1596 smears with negativity in 1072 and unacceptable reactions in 207 samples...
February 13, 2018: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/29438168/utility-of-ck7-versus-p16-as-a-prognostic-biomarker-in-cin-2
#5
Brandon Umphress, Beatriz Sanchez, Ajit Paintal, Ritu Nayar, Kruti P Maniar
Cervical intraepithelial neoplasia (CIN) 2 is an equivocal diagnosis, with p16 immunohistochemical positivity currently recommended for diagnostic confirmation. Biomarkers characteristic of squamocolumnar junction cells were recently found to be positive in almost all CIN 2 and CIN 3. CIN 1 lesions which express squamocolumnar junction markers (in particular cytokeratin 7 [CK7]) are associated with a higher rate of subsequent high-grade squamous intraepithelial lesion, suggesting that CK7 may be a useful prognostic biomarker for CIN 1...
February 12, 2018: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29437696/clinical-outcomes-after-conservative-management-of-cervical-intraepithelial-neoplasia-grade-2-cin2-in-women-ages-21-39-years
#6
Michelle I Silver, Julia C Gage, Mark Schiffman, Barbara Fetterman, Nancy E Poitras, Thomas Lorey, Li C Cheung, Hormuzd A Katki, Alexander Locke, Walter K Kinney, Philip E Castle
Cervical intraepithelial neoplasia grade 2 (CIN2) frequently regresses, is typically slow-growing, and rarely progresses to cancer. Some women forgo immediate treatment, opting for conservative management (heightened surveillance with cytology and colposcopy), to minimize overtreatment and increased risk of obstetric complications; however, there are limited data examining clinical outcomes in these women. We performed a retrospective cohort analysis of younger women diagnosed with initially untreated CIN1/2, CIN2 and CIN2/3 lesions at Kaiser Permanente Northern California between 2003-2015...
February 5, 2018: Cancer Prevention Research
https://www.readbyqxmd.com/read/29434935/methylation-of-the-l1-gene-and-integration-of-human-papillomavirus-16-and-18-in-cervical-carcinoma-and-premalignant-lesions
#7
Francisco Israel Torres-Rojas, Luz Del Carmen Alarcón-Romero, Marco Antonio Leyva-Vázquez, Julio Ortiz-Ortiz, Miguel Ángel Mendoza-Catalán, Daniel Hernández-Sotelo, Oscar Del Moral-Hernández, Hugo Alberto Rodríguez-Ruiz, Dinorah Leyva-Illades, Eugenia Flores-Alfaro, Berenice Illades-Aguiar
High-risk human papillomavirus (HPV) is the primary cause of cervical carcinoma (CC). Viral integration into the host chromosomes is associated with neoplastic progression, and epigenetic changes may occur as a result. The objective of the present study was to analyze HPV L1 gene methylation and to compare the use of quantitative polymerase chain reaction (qPCR), in situ hybridization (ISH) and L1 methylation analysis as methods for detecting HPV integration. Cervical scrapes or biopsy samples positive for HPV 16 or 18, from 187 female patients with CC, squamous intraepithelial lesions (SILs) or no intraepithelial lesion (non-IL) were analyzed...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434897/folate-deficiency-and-aberrant-dna-methylation-and-expression-of-fhit-gene-were-associated-with-cervical-pathogenesis
#8
Qiaoling Li, Ling Ding, Nan Jing, Chunliang Liu, Zuokai Yang, Fang Chen, Lifang Hou, Jintao Wang
Aberrant DNA methylation is a recognized feature in various types of human cancer, and folate has a vital role in the epigenetics of mammalian cells by supplying methyl groups for DNA methylation reactions. Fragile histidine triad (FHIT) is a tumor suppressor gene that is frequently silenced in cervical cancer (CC) and preneoplastic lesions. Promoter hypermethylation was previously observed in CC, and its epigenetic silencing has been observed at mRNA or protein levels. Changes in folate intake to modulate DNA methylation may be a mechanistic link to cancer, but this remains to be elucidated...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434847/the-relationship-between-cervical-precancerous-lesion-galectin-3-and-p27-protein-expression-and-clinical-prognosis
#9
Jianting Ma, Xingguang Zhang, Gang He, Chunlin Yang
The relationship between galectin-3 and p27kip1 protein expression levels in cervical precancerous lesions and clinical prognosis were studied. A total of 74 patients with cervical intraepithelial neoplasia [(CIN), 20 cases classified as stage I, 24 cases as stage II and 30 cases as stage III] were enrolled in this study. Tissue galectin-3, p27kip1, vascular endothelial growth factor (VEGF)-2 and cyclin D protein levels were detected via immunohistochemical staining and reverse transcription polymerase chain reaction (PCR)...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434342/p16-immunostaining-in-histological-grading-of-anal-squamous-intraepithelial-lesions-a-systematic-review-and-meta-analysis
#10
Andreia Albuquerque, Elisabete Rios, Claudia Camila Dias, Mayura Nathan
p16 is the most widely studied biomarker in lower anogenital tract squamous intraepithelial lesions and, currently the only recommended biomarker for histological grade assessment. The aim of this systematic review and meta-analysis was to evaluate p16-positive rates according to anal squamous intraepithelial lesions/anal intraepithelial neoplasia (AIN) grade. Two investigators independently searched four electronic databases: PubMed, Web of Sciences, Scopus, and Embase from inception until August 2017. Studies that evaluated p16 immunostaining in histological samples of anal and/or perianal squamous intraepithelial lesions and defined a p16-positive result as diffuse block staining with nuclear or nuclear plus cytoplasmic staining were included...
February 13, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29433016/self-sampling-with-hpv-mrna-analyses-from-vagina-and-urine-compared-with-cervical-samples
#11
Katrin Christine Asciutto, Avalon Ernstson, Ola Forslund, Christer Borgfeldt
BACKGROUND: In order to increase coverage in the organized cervical screening program, self-sampling with HPV analyses has been suggested. OBJECTIVES: The aim was to compare human papillomavirus (HPV) mRNA detection in vaginal and urine self-collected samples with clinician-taken cervical samples and the corresponding clinician-taken histological specimens. STUDY DESIGN: Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 209 women with the Aptima mRNA assay (Hologic Inc, MA, USA)...
February 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29430635/hpv-testing-vs-hpv-cytology-co-testing-to-predict-the-outcome-after-conization
#12
Laerke Valsøe Bruhn, Sisse Josephine Andersen, Jalil Hariri
INTRODUCTION: The purpose of this study was to determine the feasibility of human papillomavirus (HPV) testing alone as prognostic tool to predict recurrent disease within a three years follow-up period after treatment for cervical intraepithelial neoplasia (CIN)2+. MATERIAL AND METHODS: Retrospectively 128 women, with histologically verified CIN2+, who had a conization performed at Southern Jutland Hospital in Denmark between January 1st 2013 and December 31st 2013 were included...
February 11, 2018: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/29428790/management-of-cervical-premalignant-lesions
#13
REVIEW
Partha Basu, Katayoun Taghavi, Shang-Ying Hu, Sushma Mogri, Smita Joshi
Treatment of cervical premalignant lesions (cervical intraepithelial neoplasia; CIN) of different grades is very effective, simple, and safe. The entire transformation zone of the cervix needs to be treated either by an ablative technique (cryotherapy or thermal ablation) or an excisional technique (large loop excision of transformation zone or cold knife conization); the choice of treatment depends on the size and location of the lesion and the type of the transformation zone. The cure rate after ablative treatment of high-grade CIN may be little lower than that after excisional treatment...
January 11, 2018: Current Problems in Cancer
https://www.readbyqxmd.com/read/29416214/prevalence-of-specific-types-of-human-papiloma-virus-in-cervical-intraepithelial-lesions-and-cervical-cancer-in-macedonian-women
#14
Irena Aleksioska-Papestiev, Vesna Chibisheva, Megi Micevska, Goran Dimitrov
Introduction: Cervical cancer is a malignancy originating in the transformation zone of the cervix, most commonly in the squamous cells. It is the fourth most common cancer in women worldwide, and the third most common cause of female cancer death. Genital human papilloma viruses (HPV) are sexually transmitted and approximately 630 milion people worldwide are infected. More than 200 genotypes, subtypes and variants have been reported, 13-15 being oncogenic type, which could be responsible for cervical intraepithelial lesions (CIN) or cancer...
February 2018: Medical Archives
https://www.readbyqxmd.com/read/29410710/identification-of-reliable-biomarkers-of-human-papillomavirus-16-methylation-in-cervical-lesions-based-on-integration-status-using-high-resolution-melting-analysis
#15
Lu Liu, Chunmei Ying, Zhen Zhao, Long Sui, Xinyan Zhang, Chunyan Qian, Qing Wang, Limei Chen, Qisang Guo, Jiangnan Wu
Background: The dynamic methylation of human papillomavirus (HPV) 16 DNA is thought to be associated with the progression of cervical lesions. Previous studies that did not consider the physical status of HPV 16 may have incorrectly mapped HPV 16 methylomes. In order to identify reliable biomarkers for squamous cervical cancer (SCC), we comprehensively evaluated the methylation of HPV 16 depending on the integration incidence of each sample. Methods: Based on the integration status of 115 HPV 16-infected patients (50 SCC, 30 high-grade squamous intraepithelial lesion [HSIL], and 35 low-grade squamous intraepithelial lesion [LSIL]) and HPV 16-infected Caski cell lines by PCR detection of integrated papillomavirus sequences, we designed a series of primers that would not be influenced by breakpoints for a high-resolution melting (HRM) PCR method to detect the genome methylation...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29402202/predicting-factors-for-high-grade-cervical-dysplasia-in-women-with-low-grade-cervical-cytology-and-nonvisible-squamocolumnar-junction
#16
Giorgio Bogani, Francesca Taverna, Claudia Lombardo, Antonino Ditto, Fabio Martinelli, Mauro Signorelli, Valentina Chiappa, Umberto Leone Roberti Maggiore, Lavinia Mosca, Ilaria Sabatucci, Cono Scaffa, Domenica Lorusso, Francesco Raspagliesi
OBJECTIVE: To assess the risk of developing high-grade cervical dysplasia among women with low-grade cervical cytology and nonvisible squamocolumnar junction (SCJ) at colposcopic examination. METHODS: Data of consecutive women with low-grade intraepithelial lesion(≤LSIL) undergoing colposcopic examination, which was unsatisfactory (due to the lack of the visualization of the entire SCJ), were retrospectively reviewed. The risk of developing high-grade cervical intraepithelial neoplasia (CIN2+) was assessed using Kaplan-Meier and Cox models...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29400024/e2-e6-ratio-and-l1-immunoreactivity-as-biomarkers-to-determine-hpv16-positive-high-grade-squamous-intraepithelial-lesions-cin2-and-3-and-cervical-squamous-cell-carcinoma
#17
Youn Jin Choi, Ahwon Lee, Tae Jung Kim, Hyun Tak Jin, Yong Bok Seo, Jong Sup Park, Sung Jong Lee
OBJECTIVE: Human papillomavirus (HPV) 16 is the most carcinogenic HPV genotype. We investigated if HPV16 L1 capsid protein and E2/E6 ratio, evaluated by cervical cytology, may be used as biomarkers of ≥cervical intraepithelial neoplasia (CIN) 2 lesions. METHODS: Cervical specimens were obtained from 226 patients with HPV16 single infection. Using cytology specimen, L1 capsid protein and E2/E6 ratio were detected and the results were compared with those of the conventional histologic analysis of cervical tissues (CIN1-3 and squamous cell carcinoma [SCC]) to evaluate the association...
February 1, 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29396352/risk-of-high-risk-human-papillomavirus-infection-and-cervical-precancerous-lesions-with-past-or-current-trichomonas-infection-a-pooled-analysis-of-25-054-women-in-rural-china
#18
Rui-Mei Feng, Margaret Z Wang, Jennifer S Smith, Li Dong, Feng Chen, Qin-Jing Pan, Xun Zhang, You-Lin Qiao, Fang-Hui Zhao
BACKGROUND: Trichomonas vaginitis (TV) infection has obviously been implicated in gynecological morbidity but still unclear in cervical lesions. OBJECTIVE: To evaluate the risk of hr-HPV infection and cervical intraepithelial neoplasia grade 2 or worse (CIN2 + ) by TV infection. STUDY DESIGN: The pooled study was conducted among 12 population-based, cervical cancer screening studies throughout China (N = 24,054). HPV was detected by Hybrid Capture®2 (HC2) test...
December 30, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29390909/colpocytological-abnormalities-in-hiv-infected-and-uninfected-pregnant-women-prevalence-persistence-and-progression
#19
Carmine Carriero, Fabiana Divina Fascilla, Paola Cramarossa, Achiropita Lepera, Stefano Bettocchi, Antonella Vimercati
In this retrospective case-control study, we analyse data of 48 HIV-positive pregnant patients, versus a control group of 99 HIV-negative pregnant women, followed as outpatients by our department from 2009 to 2014. The aims of the study were to investigate the prevalence, persistence and progression of cervical squamous intraepithelial lesions (SIL) in each group and to correlate colpo-cytological lesions to the socio-demographic and clinical-laboratory findings in the HIV + pregnant women. In our study we observed that immunosuppression, HPV infection and vaginal coinfections were predictive of cervical lesions...
February 1, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29387223/telomerase-activity-in-cervical-scrapes-of-women-with-high-grade-cervical-disease-a-nested-case-control-study
#20
Mónica Molano, Diana Carolina Martín, Pablo Moreno-Acosta, Gustavo Hernández, Alyssa Cornall, Oscar Buitrago, Oscar Gamboa, Suzanne Garland, Sepehr Tabrizi, Nubia Muñoz
Epidemiological information on telomerase activity (TA) and development of cervical lesions is scarce. A nested case-control study was carried out within a cohort of Colombian women tested for Human Papillomavirus (HPV). Measurement of TA was done in cervical scrapes of 25 women who developed High Grade Squamous Intraepithelial Lesion (HGSIL) during the first 6 years of follow-up and was compared with that of 104 control women who maintained normal cytology during the entire follow-up. TA was measured by a telomerase repeat amplification protocol-ELISA...
January 2018: Oncology Letters
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