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Cervical intraepithelial lesions

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https://www.readbyqxmd.com/read/29679470/molecular-progression-to-cervical-precancer-epigenetic-switch-or-sequential-model
#1
Belinda Nedjai, Caroline Reuter, Amar Ahmad, Rawinder Banwait, Rhian Warman, James Carton, Sabrina Boer, Jack Cuzick, Attila Lorincz
The evolution of precancerous cervical lesions is poorly understood. A widely held model of cervical intraepithelial neoplasia grade 3 (CIN3) development is sequential progression from normal through CIN1 and CIN2 to CIN3. Another hypothesis, the "molecular switch" model, postulates that CIN3 can evolve directly from human papillomavirus (HPV)-infected normal epithelium without progressing through CIN1 and CIN2. To shed light on this process, we compared DNA methylation of selected human biomarkers and HPV types in two groups of CIN1: CIN1 that were near or adjacent to CIN3 (adjacent-CIN1) and CIN1 that were the principal lesions with no CIN3 detected (principal-CIN1)...
April 21, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29678360/validation-of-a-new-hpv-self-sampling-device-for-cervical-cancer-screening-the-cervical-and-self-sample-in-screening-cassis-study
#2
Mariam El-Zein, Sheila Bouten, Karolina Louvanto, Lucy Gilbert, Walter Gotlieb, Robert Hemmings, Marcel A Behr, Eduardo L Franco, Victoria Liang, Claudia Martins, Silvy Duarte, Natalia Sarban, Patricia Geddes, Ana Massa, Kathrin Samios, Siham Aboufadl, Sophie Verdon, Cynthia Pereria, Isabelle Lacroix
OBJECTIVE: We compared the self-sampling performance of the newly designed HerSwab™ device with a physician-collected cervical sample and another self-sample using the cobas® PCR Female swab for the detection of cervical intraepithelial neoplasia (CIN) and cancer. METHODS: Women referred for colposcopy at McGill University affiliated hospital clinics collected two consecutive self-samples, one with HerSwab™ and one with cobas® swab, after receiving instructions...
April 17, 2018: Gynecologic Oncology
https://www.readbyqxmd.com/read/29673740/recent-intrauterine-device-use-and-the-risk-of-precancerous-cervical-lesions-and-cervical-cancer
#3
Sarah Averbach, Michael J Silverberg, Wendy Leyden, Karen Smith-McCune, Tina Raine-Bennett, George F Sawaya
OBJECTIVE: Understanding the effect of contraceptives on the development of precancerous lesions of the cervix and cervical cancer may provide information that is valuable to women in contraceptive decision-making. The purpose of this study was to evaluate the association between recent intrauterine device (IUD) use (by type) and cervical intraepithelial neoplasia 2, 3, adenocarcinoma in situ or cancer (CIN2+ or CIN3+). STUDY DESIGN: Case-control study of 17,559 women age 18-49 with incident CIN2+ cases and 5:1 age-matched, incidence-density selected controls (N=87,378) who were members of Kaiser Permanente Northern California Healthcare System from 1996-2014...
April 16, 2018: Contraception
https://www.readbyqxmd.com/read/29671553/cervical-cancer-screening
#4
Caitlyn M Rerucha, Rebecca J Caro, Vernon L Wheeler
Screening in women has decreased the incidence and mortality of cervical cancer. Precancerous cervical lesions (cervical intraepithelial neoplasias) and cervical carcinomas are strongly associated with sexually-transmitted high-risk human papillomavirus (HPV) infection, which causes more than 99% of cervical cancers. Screening methods include cytology (Papanicolaou test) and HPV testing, alone or in combination. The American Academy of Family Physicians and the U.S. Preventive Services Task Force recommend starting screening in immunocompetent, asymptomatic women at 21 years of age...
April 1, 2018: American Family Physician
https://www.readbyqxmd.com/read/29664054/limited-role-of-promoter-methylation-of-mgmt-and-c13orf18-in-triage-of-low-grade-squamous-intraepithelial-lesion
#5
Lu-Lu Sun, Yuan Liu, Xiao Sun, Lei Pan, Dan Wu, Yu-Dong Wang
Background: Promoter methylation of MGMT and C13ORF18 has been confirmed as a potential biomarker for early diagnosis of cervical cancer. The aim of this study was to evaluate the performance of MGMT and C13ORF18 promoter methylation for triage of cytology screening samples and explore the potential mechanism. Methods: Methylation-sensitive high-resolution melting was used to detect promoter methylation of MGMT and C13ORF18 in 124 cervical samples. High-risk human papillomavirus (HR-HPV) was detected by the Digene Hybrid Capture 2® ...
April 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29658966/methylation-in-the-promoter-regions-of-wt1-nkx6-1-and-dbc1-genes-in-cervical-cancer-tissues-of-uygur-women-in-xinjiang
#6
Dan Wu, Jinli Zhang, Peiwen Fan, Hongtao Li, Dongmei Li, Huan Pan, Hongchang He, Xianxian Ren, Zhenzhen Pan, Renfu Shao, Zemin Pan
This study aimed to explore: 1) DNA methylation in the promoter regions of Wilms tumor gene 1 (WT1), NK6 transcription factor related locus 1 gene (NKX6-1) and Deleted in bladder cancer 1 (DBC1) gene in cervical cancer tissues of Uygur women in Xinjiang, and 2) the correlation of gene methylation with the infection of HPV16/18 viruses. We detected HPV16/18 infection in 43 normal cervical tissues, 30 cervical intraepithelial neoplasia lesions (CIN) and 48 cervical cancer tissues with polymerase chain reaction (PCR) method...
January 2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29655523/the-clinical-value-of-hpv-e6-e7-and-stat3-mrna-detection-in-cervical-cancer-screening
#7
Yibing Fan, Zongji Shen
OBJECTIVE: To explore the value of human papillomavirus (HPV) E6/E7 and signal transducer and activator of transcription 3 (STAT3) mRNA detection in the screening of cervical lesions. METHODS: 192 patients with abnormal ThinPrep cytology test (TCT) results and/or high-risk HPV infection were screened to identify possible cervical lesions in cases. Diagnoses were confirmed by histopathology. Fluorescence in situ hybridization (FISH) was performed to detect and qualify the mRNAs of HPV E6/E7, STAT3, and Survivin in cervical exfoliated cells...
February 11, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29652189/a-review-and-update-on-papillary-immature-metaplasia-of-the-uterine-cervix-a-distinct-subset-of-low-grade-squamous-intraepithelial-lesion-proposing-a-possible-cell-of-origin
#8
Soon Auck Hong, Su Hyun Yoo, Jene Choi, Stanley J Robboy, Kyu-Rae Kim
CONTEXT: - Papillary immature metaplasia (PIM) is a known papillary cervical lesion associated with low-risk human papilloma virus (LR-HPV). OBJECTIVE: - To evaluate additional clinicopathologic features and the HPV genotypes of PIM and discuss the presumptive cell of origin. DESIGN: - A total of 26 PIM cases were evaluated by p16INK4a , cytokeratin (CK) 7, and CK17 immunohistochemical stainings. Human papilloma virus genotyping was performed, by using HPV DNA Chip, HPV polymerase chain reaction (PCR), and real-time PCR...
April 13, 2018: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29643651/pilot-study-of-an-open-source-image-analysis-software-for-automated-screening-of-conventional-cervical-smears
#9
Parikshit Sanyal, Prosenjit Ganguli, Sanghita Barui, Prabal Deb
Introduction: The Pap stained cervical smear is a screening tool for cervical cancer. Commercial systems are used for automated screening of liquid based cervical smears. However, there is no image analysis software used for conventional cervical smears. The aim of this study was to develop and test the diagnostic accuracy of a software for analysis of conventional smears. Materials and Methods: The software was developed using Python programming language and open source libraries...
April 2018: Journal of Cytology
https://www.readbyqxmd.com/read/29627221/fluorescence-microscopy-of-h-e-stained-cervical-biopsies-to-assist-the-diagnosis-and-grading-of-cin
#10
REVIEW
Mario R Castellanos, Vijeyaluxmy Motilal Nehru, Edyta C Pirog, Lynne Optiz
BACKGROUND: Prevention of cervical cancer is based upon the accurate diagnosis and grading of cervical lesions identified during screening. The pathological classification of cervical intraepithelial neoplasia (CIN) is problematic, as it relies on subjective criteria and is known to have high interobserver variability and low reproducibility. These limitations can result in either over or under treatment of patients. Biomarkers to improve CIN diagnosis have not overcome all these challenges...
March 26, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29619426/genotyping-of-human-pappilomavirus-in-cervical-precancerous-lesion-and-squamous-cell-carcinoma-at-dr-soetomo-hospital-surabaya-indonesia
#11
Gondo Mastutik, Rahmi Alia, Alphania Rahniayu, Anny Setijo Rahaju, Nila Kurniasari, Suhartono Taat Putra
Background: Cervical cancer caused by human papilloma virus (HPV), is the second most common cancer for women. This cancer is distributed worldwide, with ~80% of cases are found in the developing countries. In Indonesia, data of HPV genotypes are still limited and do not represent all regions of the country. Thus, here we report genotyping of HPV samples collected from the Dr. Soetomo Hospital Surabaya Indonesia patients, in 2013. Materials and Method: A cross sectional study was performed using 68 paraffin blocks of low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) cervix...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29617952/risk-factors-for-human-papillomavirus-infection-and-abnormal-cervical-cytology-among-perinatally-human-immunodeficiency-virus-infected-and-uninfected-asian-youth
#12
Annette H Sohn, Stephen J Kerr, Rawiwan Hansudewechakul, Sivaporn Gatechompol, Kulkanya Chokephaibulkit, Hanh Le Dung Dang, Dan Ngoc Hanh Tran, Jullapong Achalapong, Nipat Teeratakulpisarn, Amphan Chalermchockcharoenkit, Manopchai Thamkhantho, Tippawan Pankam, Thida Singtoroj, Wichai Termrungruanglert, Surasith Chaithongwongwatthana, Nittaya Phanuphak
Background: Infection with high-risk human papillomavirus (HR-HPV) may be higher in perinatally human immunodeficiency virus (HIV)-infected (PHIV) than HIV-uninfected (HU) adolescents because of long-standing immune deficiency. Methods: PHIV and HU females aged 12-24 years in Thailand and Vietnam were matched by age group and lifetime sexual partners. At enrollment, blood, cervical, vaginal, anal, and oral samples were obtained for HPV-related testing. The Wilcoxon and Fisher exact tests were used for univariate and logistic regression for multivariate analyses...
March 30, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29609231/-characteristics-of-invisible-cervical-intraepithelial-neoplasia-%C3%A2-under-colposcopy
#13
Q Chen, H Du, C Wang, J L Tang, R F Wu
Objective: To explore the human papilomavirus (HPV) genotypes and epithelial thickness of invisible cervical intraepithelial neoplasia Ⅲ (CIN Ⅲ) under colposcopy. Methods: One hundred and sixty-nine biopsies from 93 patients with a final diagnosis of CIN Ⅲ were extracted from the Shenzhen cervical cancer screening trial Ⅱ (SHENCCAST Ⅱ) . The SHENCCAST Ⅱ was conducted from 2009 to 2010. All the cervical blocks from these patients were re-cut and placed on 6 slides, i.e. sandwich model, with the top and bottom sections being stained with HE, the top second be processed for other studies, 3 sections for HPV genotypes by matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) assay...
March 25, 2018: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/29603728/distinct-profiles-of-tert-promoter-mutations-and-telomerase-expression-in-head-and-neck-cancer-and-cervical-carcinoma
#14
Clorinda Annunziata, Francesca Pezzuto, Stefano Greggi, Franco Ionna, Simona Losito, Gerardo Botti, Luigi Buonaguro, Franco M Buonaguro, Maria Lina Tornesello
Two recurrent mutations (-124 G>A and -146 G>A) in the core promoter region of the human telomerase reverse transcriptase (TERT) gene create consensus binding sites for ETS transcription factors and cause increased TERT expression in several tumour types. We analyzed TERT promoter mutations and TERT mRNA levels in head and neck cancer, cervical carcinoma and cervical intraepithelial neoplasia (CIN) as well as in C-4I, CaSki, HeLa and SiHa cervical cell lines. Nucleotide sequence analysis of TERT promoter region showed that 33...
March 31, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29599374/natural-history-of-squamous-intraepithelial-lesions-in-pregnancy-and-mode-of-delivery
#15
Stefanie Schuster, Elmar Joura, Petra Kohlberger
BACKGROUND: Numerous studies have addressed the impact of mode of delivery on the natural history of squamous intraepithelial lesions (SIL) in pregnant women. However, the literature is still contradictory. PATIENTS AND METHODS: In the course of a retrospective analysis, data of 63 pregnant women with abnormal cervical smears who were referred to our Outpatient Department for pre-invasive lesions of the cervix were analyzed. The study was conducted at the General Hospital in Vienna, Austria, between 2010 and 2015...
April 2018: Anticancer Research
https://www.readbyqxmd.com/read/29597202/impact-of-implementing-100-rapid-review-as-a-quality-control-tool-in-cervical-cytology
#16
Juliana Cristina Magalhães, Cinara Zago Silveira Ázara, Sulene Brito do Nascimento Tavares, Edna Joana Claudio Manrique, Rita Goreti Amaral
OBJECTIVE: We aimed to evaluate the impact of implementing 100% rapid review (100% RR) as a quality control tool in cervical smear cytology. DESIGN: A cross-sectional study was conducted in which cytology findings, false-negative results, and quality indicators were evaluated. The variables were analyzed in 2004, the year in which 100% RR was implemented, and again in 2013, i.e., 10 years on. RESULTS: Detection of atypical squamous cells of undetermined significance (ASC-US) increased from 0...
March 29, 2018: Acta Cytologica
https://www.readbyqxmd.com/read/29593040/within-host-variations-of-human-papillomavirus-reveal-apobec-signature-mutagenesis-in-the-viral-genome
#17
Yusuke Hirose, Mamiko Onuki, Yuri Tenjimbayashi, Seiichiro Mori, Yoshiyuki Ishii, Takamasa Takeuchi, Nobutaka Tasaka, Toyomi Satoh, Tohru Morisada, Takashi Iwata, Shingo Miyamoto, Koji Matsumoto, Akihiko Sekizawa, Iwao Kukimoto
Persistent infection with oncogenic human papillomaviruses (HPVs) causes cervical cancer, accompanied with the accumulation of somatic mutations into the host genome. There are concomitant genetic changes in the HPV genome during viral infection; however, their relevance to cervical carcinogenesis is poorly understood. Here we explored within-host genetic diversity of HPV by performing deep sequencing analyses of viral whole-genome sequences in clinical specimens. The whole genomes of HPV types 16, 52 and 58 were amplified by type-specific PCR from total cellular DNA of cervical exfoliated cells collected from patients with cervical intraepithelial neoplasia (CIN) and invasive cervical cancer (ICC), and were deep-sequenced...
March 28, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29578257/an-automated-quantitative-dna-image-cytometry-system-detects-abnormal-cells-in-cervical-cytology-with-high-sensitivity
#18
O G Wong, M W Ho, O K Tsun, A K Ng, E Y Tsui, J N Chow, P P Ip, A N Cheung
OBJECTIVE: To evaluate the performance of an automated DNA-image-cytometry system as a tool to detect cervical carcinoma. METHODS: Of 384 liquid-based cervical cytology samples with available biopsy follow-up were analyzed by both the Imager System and a high-risk HPV test (Cobas). RESULTS: The sensitivity and specificity of Imager System for detecting biopsy proven high-grade squamous intraepithelial lesion (HSIL, cervical intraepithelial neoplasia [CIN]2-3) and carcinoma were 89...
March 26, 2018: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/29578171/effect-of-smoking-on-high-grade-cervical-cancer-in-women-on-the-basis-of-human-papillomavirus-infection-studies
#19
Jian-Hong Fang, Xue-Mei Yu, Shu-Hong Zhang, Yan Yang
Purpose: We aimed, in the present study, to measure the risk related to the high-grade cervical intraepithelial neoplasia grade 3 (CIN3) or worse (CIN3+) or worse/high-grade squamous intraepithelial lesions with respect to changes in human papillomavirus (HPV) and smoking status. Materials and Methods: A structured interview underwent for 7129 women. Then, we obtained their cervical cells and subjected to HPV testing. High-risk HPV infected and "no prevalent" cervical disease infected women were followed for cervical lesions up to 12 years (at baseline; n = 1531)...
2018: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/29575971/prognosis-and-related-factors-of-hpv-infections-in-postmenopausal-uyghur-women
#20
Shuang Sui, Mingyue Zhu, Zhen Jiao, Lili Han, Lin Wang, Mayineur Niyazi, Kaichun Zhu
With the aim to explore the characteristics of persistent HPV infections in postmenopausal Uyghur women and analyse the possible related risk factors, from September 2012 to September 2013; postmenopausal Uyghur women with HPV positive and pathologically diagnosed as non-cervical intraepithelial neoplasia (CIN) lesions and non-cervical cancer were recruited. Their clinical course was closely followed up for 24-36 months, and the risk factors were analysed by a logistic regression model. One hundred and sixteen positive women were followed for 36 months...
March 25, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
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