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Zehao Li, Xu Zhou, Lidan Li, Shue Liu, Congshan Wang, Lina Li, Changyuan Yu, Xin Su
Hybridization-based probes emerge as a promising tool for nucleic acid target detection and imaging. However, the single-nucleotide selectivity is still challenging because the specificity of hybridization reaction is typically low at room temperature. We disclose an effective and simple method for highly selective detection and in situ imaging of single-nucleotide mutation (SNM) by taking the advantages of the specific hybridization of short duplex and the signal amplifying effect of cationic conjugated polymer (CCP)...
May 16, 2018: Analytical Chemistry
Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Manman Geng, Xiaoying Wu, Safdar Hussain, Bo Wang, Muhammad Shahid Riaz Rajoka, Yue Li, Juan Tian, Liesu Meng, Shemin Lu
Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of the present study was to observe the role of miR-10a-5p targeting T-box transcription factor 5 (TBX5) gene on synoviocyte proliferation and apoptosis in RA. Human synovial sarcoma cell line, SW982 cells stimulating with interleukin-1β (IL-1β) were transfected with miR-10a-5p mimic and siRNA of TBX5...
April 27, 2018: Bioscience Reports
Jianhai Yin, Congshan Liu, Yujuan Shen, Haobing Zhang, Jianping Cao
BACKGROUND: Cystic echinococcosis is a global public health problem; however, the drugs (albendazole and mebendazole) currently recommended by WHO for its treatment, have limited efficacy. Therefore, novel drugs are required to provide more choices for the treatment of this disease. METHODS: The anthelmintic effects of ursolic acid (UA) were tested on Echinococcus granulosus protoscoleces, germinal cells and metacestodes in vitro. The in vivo efficacy of UA was investigated in mice following secondary infection with E...
January 24, 2018: Parasites & Vectors
Biquan Xiong, Gang Wang, Congshan Zhou, Yu Liu, Panliang Zhang, Kewen Tang
An efficient phosphorylation of C(sp3 )-H bonds of arenes with diaryl phosphinic acids via Bu4 NI-catalyzed dehydrogenative coupling has been developed. This transformation proceeds efficiently under transition-metal-free reaction conditions and represents a straightforward method to prepare valuable organophosphorus compounds from readily available arenes and diaryl phosphinic acids.
January 19, 2018: Journal of Organic Chemistry
Bo Wang, Panpan Zhao, Yan Zhou, Liesu Meng, Wenhua Zhu, Congshan Jiang, Linyu Wang, Yongsong Cai, Shemin Lu, Weikun Hou
The objective of this study was to identify the key changed subtype of T helper cells (Th cells) and their cytokines in pristane-induced arthritis (PIA) in rats. The severity of arthritis was evaluated by body weight, clinical score, the perimeter of ankle and mid-paw and histological assessment of ankle joints. Cytokines of Th1, Th2 and Th17 were determined in the spleen and inguinal lymph nodes at 28 days after pristane injection by real-time qPCR. The mRNA levels of IL-22 receptors, IL-22R1 and IL-22BP, in the spleen were quantified by real-time qPCR...
2017: PloS One
Yasheng Yan, Shigang Qiao, Chika Kikuchi, Ivan Zaja, Sarah Logan, Congshan Jiang, Thiago Arzua, Xiaowen Bai
It has been shown that propofol can induce widespread apoptosis in neonatal mouse brains followed by long-term cognitive dysfunction. However, selective brain area and cell vulnerability to propofol remains unknown. This study was aimed to dissect toxic effect of propofol on multiple brain cells, including neurons, astrocytes, oligodendrocytes, and neural stem cells (NSCs). Seven-day-old mice were intraperitoneally administrated propofol or intralipid as a vehicle control for 6 hours. To identify vulnerable cells undergoing apoptosis following propofol exposure, brain sagittal sections were co-stained with antibodies against an apoptosis marker along with neuron, astrocyte, oligodendrocyte, or NSC markers using immunofluorescence staining...
October 14, 2017: Brain Sciences
Congshan Liu, Jianhai Yin, Jian Xue, Yi Tao, Wei Hu, Haobing Zhang
Cystic echinococcosis is a globally distributed zoonotic disease, which is caused by the larval stage of Echinococcosus granulosus sensu lato. The chemotherapy of the disease is limited to the use of benzimidazoles. Recently, mefloquine and its analogues, aminoalcohol-carbazole, and some amino alcohol derivatives were reported to display inhibitory effects on parasites. Here, the activities of 130 amino alcohol compounds against E. granulosus were tested on protoscoleces and germinal cells at a concentration of 20μg/ml over a period of three days...
August 28, 2017: Acta Tropica
Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Xiaoying Wu, Manman Geng, Safdar Hussain, Yongsong Cai, Ke Xu, Peng Xu, Yan Han, Jian Sun, Liesu Meng, Shemin Lu
MicroRNAs are considered to play critical roles in the pathogenesis of human inflammatory arthritis, including rheumatoid arthritis (RA). The purpose of this study was to determine the relationship between miR-10a-5p and TBX5 in synoviocytes and evaluate their contribution to joint inflammation. The expression of miR-10a-5p and TBX5 in the synovium of RA and human synovial sarcoma cell line SW982 stimulated by IL-1β was determined by RT-qPCR and Western blotting. The direct interaction between miR-10a-5p and TBX5 3'UTR was determined by dual-luciferase reporter assay in HeLa cells...
January 2018: Journal of Cellular and Molecular Medicine
Congshan Zhuo, Pierre Sagaut
In this paper, a variant of the acoustic multipole source (AMS) method is proposed within the framework of the lattice Boltzmann method. A quadrupole term is directly included in the stress system (equilibrium momentum flux), and the dependency of the quadrupole source in the inviscid limit upon the fortuitous discretization error reported in the works of E. M. Viggen [Phys. Rev. E 87, 023306 (2013)PLEEE81539-375510.1103/PhysRevE.87.023306] is removed. The regularized lattice Boltzmann (RLB) method with this variant AMS method is presented for the 2D and 3D acoustic problems in the inviscid limit, and without loss of generality, the D3Q19 model is considered in this work...
June 2017: Physical Review. E
Weikun Hou, Bo Wang, Yan Zhou, Ke Xu, Liesu Meng, Wenhua Zhu, Congshan Jiang, Peng Xu, Shemin Lu
The aim of the present study was to investigate the expression pattern of T helper (Th) 17 and Th22 cell-related factors in a pristane‑induced arthritis (PIA) rat model. PIA rats were divided into the initial phase group [day (D) 6 post‑pristane injection], the onset of clinical arthritis group (D12), the acute arthritis group (D26) and the chronic arthritis group (D70). Rats injected with saline alone were used as the control group. The mRNA expression levels of interleukin (IL)‑17A, IL‑17F, interferon (IFN)‑γ, IL‑22, IL‑22 receptor (R) 1, IL‑22 binding protein (BP) and RAR‑related orphan receptor α were examined in the spleen and/or synovium of the various phases of PIA rats by reverse transcription‑quantitative polymerase chain reaction analysis...
August 2017: Molecular Medicine Reports
Ji Li, Chengwen Zhong, Yong Wang, Congshan Zhuo
In our study, the dual time-stepping strategy of the gas-kinetic scheme is constructed and used for the simulation of unsteady flows. In comparison to the previous implicit gas-kinetic scheme, both the inviscid and viscous flux Jacobian are considered in our work, and the linear system of the pseudo-steady-state is solved by applying generalized minimal residual algorithm. The accuracy is validated by several numerical cases, the incompressible flow around blunt bodies (stationary circular cylinder and square cylinder), and the transonic buffet on the NACA0012 airfoil under hybrid mesh...
May 2017: Physical Review. E
Congshan Sun, Vanessa De Mello, Abdalla Mohamed, Huascar P Ortuste Quiroga, Amaya Garcia-Munoz, Abdullah Al Bloshi, Annie M Tremblay, Alexander von Kriegsheim, Elaina Collie-Duguid, Neil Vargesson, David Matallanas, Henning Wackerhage, Peter S Zammit
Hippo pathway downstream effectors Yap and Taz play key roles in cell proliferation and regeneration, regulating gene expression especially via Tead transcription factors. To investigate their role in skeletal muscle stem cells, we analyzed Taz in vivo and ex vivo in comparison with Yap. Small interfering RNA knockdown or retroviral-mediated expression of wild-type human or constitutively active TAZ mutants in satellite cells showed that TAZ promoted proliferation, a function shared with YAP. However, at later stages of myogenesis, TAZ also enhanced myogenic differentiation of myoblasts, whereas YAP inhibits such differentiation...
August 2017: Stem Cells
Congshan Wan, Thomas K Gaylord, Muhannad S Bakir
The interlayer waveguide grating coupling efficiencies under angular (rotational) misalignments are simulated using the 3D rigorous coupled-wave analysis (3D-RCWA) together with the RCWA equivalent-index-slab (RCWA-EIS) method. As examples of conical diffraction, rotations about the two coordinate axes, x and z, defined by the vectors [1 0 0] and [0 0 1], respectively, as well as an arbitrary axis, defined by the vector [2 2 1], are simulated for binary rectangular-groove gratings. The interlayer grating coupling efficiency is approximated by the product of the top- and bottom-grating diffraction efficiencies (DEs)...
December 10, 2016: Applied Optics
Wenhua Zhu, Jing Xu, Congshan Jiang, Bo Wang, Manman Geng, Xiaoying Wu, Nazim Hussain, Ning Gao, Yan Han, Dongmin Li, Xi Lan, Qilan Ning, Fujun Zhang, Rikard Holmdahl, Liesu Meng, Shemin Lu
Autophagy is involved in both innate and adaptive immune regulation. We propose that autophagy regulates activation of TLR3 in macrophages and is thereby essential for development of pristane-induced arthritis. We found that pristane treatment induced autophagy in macrophages in vitro and in vivo, in spleen cells from pristane injected rats. The induced autophagy was associated with STAT1 phosphorylation and expression of IRF1 and TLR3. Blocking the pristane activated autophagy by Wortmannin and Bafilomycin A1 or by RNAi of Becn1 led to a downregulation of the associated STAT1-IRF1-TLR3 pathway...
February 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Yongsong Cai, Peng Xu, Le Yang, Ke Xu, Jialin Zhu, Xiaoqing Wu, Congshan Jiang, Qiling Yuan, Bo Wang, Yuanbo Li, Yusheng Qiu
Oxymatrine (OMT) is a type of alkaloid extracted from a traditional Chinese medicinal herb, Sophora flavescens. Although the antitumor activities of OMT have been observed in various cancers, there are no reports regarding the effects of OMT on human synovial sarcoma. In the present study, we analyzed the antitumor activities of OMT in SW982 human synovial sarcoma cells and determine whether high mobility group box protein 1 (HMGB1)-mediated autophagy was associated with its therapeutic effects. We found that OMT exhibited antitumor activity in SW982 cells and facilitated increases in autophagy...
November 29, 2016: Scientific Reports
Xudong Ma, Jianfeng Xie, Yi Yang, Fengmei Guo, Zhiwei Gao, Hua Shao, Yingzi Huang, Congshan Yang, Haibo Qiu
BACKGROUND: China's Ministry of Health (MOH) has established a policy about the antimicrobial stewardship. To date, the effects of this policy on multidrug-resistant organism (MDRO) in critically ill patients are unknown. METHODS: A pre-post study was conducted on intensive care unit (ICU) patients from June 2010 to May 2011 and from June 2012 to May 2013. Bacterial cultures were conducted at ICU admission and discharge. In June 2011, our hospital started to administer the antimicrobial stewardship program of Chinese MOH...
November 25, 2016: BMC Infectious Diseases
Congshan Wan, Thomas K Gaylord, Muhannad S Bakir
Corrections to Eqs. (25) and (32) in our recent publication [Appl. Opt.55, 2601-2610 (2016).APOPAI0003-693510.1364/AO.55.002601] are presented in this erratum.
October 1, 2016: Applied Optics
Jing Xu, Wenhua Zhu, Yuanxu Guo, Congshan Jiang, Nazim Hussain, Liesu Meng, Shemin Lu
The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescence-activated cell sorting (FACS). In the present work, PCR, restriction endonuclease digestion and molecular cloning techniques were used to insert the gene decoding green fluorescent protein (GFP) without changing the structure of original plasmid to extend its application...
February 2017: Applied Biochemistry and Biotechnology
Shanshan Wu, Rui Zhang, Fayi Nie, Xiaoli Wang, Congshan Jiang, Meng Liu, Robert K Valenzuela, Wanqing Liu, Yongyong Shi, Jie Ma
MicroRNAs (miRNAs) are a class of endogenous and non-coding single-stranded RNAs of approximately 22 nucleotides, many of which are evolutionarily conserved. Genome-wide association studies have identified a robust statistical association between the MIR137 gene and schizophrenia in Europeans, which was replicated in the Han Chinese population in a case-control study. In the previous study, we provided evidence for a significant association between the EFNB2 gene and schizophrenia in Han Chinese subjects. In the current study, we utilized computational analysis, vector construction of point mutations, luciferase reporter assays and gene expression assays including RT-qPCR and western blotting methods to investigate miR-137 directly targeting EFNB2 gene and explore the reversal effect of a genetic variant of SNP rs550067317 in the putative seed-pair region of EFNB2 3'-UTR...
October 2016: EBioMedicine
Congshan Wan, Thomas K Gaylord, Muhannad S Bakir
The grating coupling efficiencies for interlayer connection (overlaid chips) were previously calculated using the new rigorous coupled-wave analysis equivalent-index-slab (RCWA-EIS) method. The chip-to-chip coupling efficiencies were determined for rectangular-groove (binary) gratings. In the present work, the search algorithms used in the RCWA-EIS method are optimized giving rise to improved definition of equivalent indices. Further, the versatility of the RCWA-EIS method is demonstrated by extending it to (nonbinary) parallelogramic gratings, sawtooth gratings, and volume gratings...
August 1, 2016: Applied Optics
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