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https://www.readbyqxmd.com/read/27897164/hmgb1-mediated-autophagy-decreases-sensitivity-to-oxymatrine-in-sw982-human-synovial-sarcoma-cells
#1
Yongsong Cai, Peng Xu, Le Yang, Ke Xu, Jialin Zhu, Xiaoqing Wu, Congshan Jiang, Qiling Yuan, Bo Wang, Yuanbo Li, Yusheng Qiu
Oxymatrine (OMT) is a type of alkaloid extracted from a traditional Chinese medicinal herb, Sophora flavescens. Although the antitumor activities of OMT have been observed in various cancers, there are no reports regarding the effects of OMT on human synovial sarcoma. In the present study, we analyzed the antitumor activities of OMT in SW982 human synovial sarcoma cells and determine whether high mobility group box protein 1 (HMGB1)-mediated autophagy was associated with its therapeutic effects. We found that OMT exhibited antitumor activity in SW982 cells and facilitated increases in autophagy...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27887595/antimicrobial-stewardship-of-chinese-ministry-of-health-reduces-multidrug-resistant-organism-isolates-in-critically-ill-patients-a-pre-post-study-from-a-single-center
#2
Xudong Ma, Jianfeng Xie, Yi Yang, Fengmei Guo, Zhiwei Gao, Hua Shao, Yingzi Huang, Congshan Yang, Haibo Qiu
BACKGROUND: China's Ministry of Health (MOH) has established a policy about the antimicrobial stewardship. To date, the effects of this policy on multidrug-resistant organism (MDRO) in critically ill patients are unknown. METHODS: A pre-post study was conducted on intensive care unit (ICU) patients from June 2010 to May 2011 and from June 2012 to May 2013. Bacterial cultures were conducted at ICU admission and discharge. In June 2011, our hospital started to administer the antimicrobial stewardship program of Chinese MOH...
November 25, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27828035/grating-design-for-interlayer-optical-interconnection-of-in-plane-waveguides-erratum
#3
Congshan Wan, Thomas K Gaylord, Muhannad S Bakir
Corrections to Eqs. (25) and (32) in our recent publication [Appl. Opt.55, 2601-2610 (2016).APOPAI0003-693510.1364/AO.55.002601] are presented in this erratum.
October 1, 2016: Applied Optics
https://www.readbyqxmd.com/read/27730520/construction-of-conveniently-screening-plko-1-trc-vector-tagged-with-turbogfp
#4
Jing Xu, Wenhua Zhu, Yuanxu Guo, Congshan Jiang, Nazim Hussain, Liesu Meng, Shemin Lu
The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescence-activated cell sorting (FACS). In the present work, PCR, restriction endonuclease digestion and molecular cloning techniques were used to insert the gene decoding green fluorescent protein (GFP) without changing the structure of original plasmid to extend its application...
October 11, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27650867/microrna-137-inhibits-efnb2-expression-affected-by-a-genetic-variant-and-is-expressed-aberrantly-in-peripheral-blood-of-schizophrenia-patients
#5
Shanshan Wu, Rui Zhang, Fayi Nie, Xiaoli Wang, Congshan Jiang, Meng Liu, Robert K Valenzuela, Wanqing Liu, Yongyong Shi, Jie Ma
MicroRNAs (miRNAs) are a class of endogenous and non-coding single-stranded RNAs of approximately 22 nucleotides, many of which are evolutionarily conserved. Genome-wide association studies have identified a robust statistical association between the MIR137 gene and schizophrenia in Europeans, which was replicated in the Han Chinese population in a case-control study. In the previous study, we provided evidence for a significant association between the EFNB2 gene and schizophrenia in Han Chinese subjects. In the current study, we utilized computational analysis, vector construction of point mutations, luciferase reporter assays and gene expression assays including RT-qPCR and western blotting methods to investigate miR-137 directly targeting EFNB2 gene and explore the reversal effect of a genetic variant of SNP rs550067317 in the putative seed-pair region of EFNB2 3'-UTR...
September 15, 2016: EBioMedicine
https://www.readbyqxmd.com/read/27505369/rcwa-eis-method-for-interlayer-grating-coupling
#6
Congshan Wan, Thomas K Gaylord, Muhannad S Bakir
The grating coupling efficiencies for interlayer connection (overlaid chips) were previously calculated using the new rigorous coupled-wave analysis equivalent-index-slab (RCWA-EIS) method. The chip-to-chip coupling efficiencies were determined for rectangular-groove (binary) gratings. In the present work, the search algorithms used in the RCWA-EIS method are optimized giving rise to improved definition of equivalent indices. Further, the versatility of the RCWA-EIS method is demonstrated by extending it to (nonbinary) parallelogramic gratings, sawtooth gratings, and volume gratings...
August 1, 2016: Applied Optics
https://www.readbyqxmd.com/read/27426724/toll-like-receptor-4-promotes-angiogenesis-in-pancreatic-cancer-via-pi3k-akt-signaling
#7
Yunliang Sun, Congshan Wu, Jianxia Ma, Yu Yang, Xiaohua Man, Hongyu Wu, Shude Li
Deregulation of Toll-like receptor 4 (TLR4) is closely associated with the progression of various types of cancers, but its role in pancreatic carcinogenesis is unclear. This study aimed to investigate the role of TLR4 in the angiogenesis of pancreatic cancer and the underlying molecular mechanisms. The culture supernatant (conditioned medium) of PANC-1 cells after appropriate treatment was used for the treatment of HUVECs. The proliferation, migration and tube formation of HUVECs were assessed by MTT, Transwell and Matrigel, respectively...
October 1, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27184927/the-hippo-effector-taz-wwtr1-transforms-myoblasts-and-taz-abundance-is-associated-with-reduced-survival-in-embryonal-rhabdomyosarcoma
#8
Abdalla Mohamed, Congshan Sun, Vanessa De Mello, Joanna Selfe, Edoardo Missiaglia, Janet Shipley, Graeme I Murray, Pete S Zammit, Henning Wackerhage
The Hippo effector YAP has recently been identified as a potent driver of embryonal rhabdomyosarcoma (ERMS). Most reports suggest that the YAP paralogue TAZ (gene symbol WWTR1) functions as YAP but, in skeletal muscle, TAZ has been reported to promote myogenic differentiation, whereas YAP inhibits it. Here, we investigated whether TAZ is also a rhabdomyosarcoma oncogene or whether TAZ acts as a YAP antagonist. Immunostaining of rhabdomyosarcoma tissue microarrays revealed that TAZ is significantly associated with poor survival in ERMS...
September 2016: Journal of Pathology
https://www.readbyqxmd.com/read/27139662/grating-design-for-interlayer-optical-interconnection-of-in-plane-waveguides
#9
Congshan Wan, Thomas K Gaylord, Muhannad S Bakir
Interlayer grating-to-grating optical interconnect coupling efficiency is simulated and optimized using rigorous coupled-wave analysis (RCWA) for the case of binary rectangular-groove gratings. The "equivalent index slab (EIS)" concept is proposed to alleviate the numerical sensitivity problem inherent in the RCWA-leaky-wave approach, making the method applicable to any multilayer structure that has an arbitrary grating profile, large refractive-index differences, and a limited grating length. The method is easy to implement and computationally efficient and can provide optimal designs based on the system designer's need...
April 1, 2016: Applied Optics
https://www.readbyqxmd.com/read/27107686/trxr2-deficiencies-promote-chondrogenic-differentiation-and-induce-apoptosis-of-chondrocytes-through-mitochondrial-reactive-oxygen-species
#10
Jidong Yan, Jing Xu, Yao Fei, Congshan Jiang, Wenhua Zhu, Yan Han, Shemin Lu
Thioredoxin reductase 2 (TrxR2) is a selenium (Se) containing protein. Se deficiency is associated with an endemic osteoarthropathy characterized by impaired cartilage formation. It is unclear whether TrxR2 have roles in cartilage function. We examined the effects of TrxR2 on chondrogenic ATDC5 cells through shRNA-mediated gene silencing of TrxR2. We demonstrated TrxR2 deficiencies could enhance chondrogenic differentiation and apoptosis of ATDC5 cells. TrxR2 deficiencies increased accumulation of cartilage glycosaminoglycans (GAGs) and mineralization...
May 15, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27075111/extracellular-microrna-21-and-microrna-26a-increase-in-body-fluids-from-rats-with-antigen-induced-pulmonary-inflammation-and-children-with-recurrent-wheezing
#11
Congshan Jiang, Hongchuan Yu, Qingzhu Sun, Wenhua Zhu, Jing Xu, Ning Gao, Rui Zhang, Li Liu, Xiaoying Wu, Xudong Yang, Liesu Meng, Shemin Lu
BACKGROUND: This study aims to find out whether extracellular miRNAs is implicated in recurrent childhood wheezing with asthmatic risk. METHODS: One hundred and forty children of Chinese Han population were recruited for this study. Plasma and intracellular miRNAs from children with recurrent wheezing and rats with antigen induced pulmonary inflammation (AIPI) were detected by using reverse transcription-quantitative PCR. Differential leukocytes in blood were automatically counted...
2016: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/27060415/regulation-of-ribosomal-rna-expression-across-the-lifespan-is-fine-tuned-by-maternal-diet-before-implantation
#12
Oleg Denisenko, Emma S Lucas, Congshan Sun, Adam J Watkins, Daniel Mar, Karol Bomsztyk, Tom P Fleming
Cells and organisms respond to nutrient deprivation by decreasing global rates of transcription, translation and DNA replication. To what extent such changes can be reversed is largely unknown. We examined the effect of maternal dietary restriction on RNA synthesis in the offspring. Low protein diet fed either throughout gestation or for the preimplantation period alone reduced cellular RNA content across fetal somatic tissues during challenge and increased it beyond controls in fetal and adult tissues after challenge release...
July 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/26951977/validation-of-reference-genes-for-quantitative-real-time-pcr-in-bovine-pbmcs-transformed-and-non-transformed-by-theileria-annulata
#13
Hongxi Zhao, Junlong Liu, Youquan Li, Congshan Yang, Shuaiyang Zhao, Juan Liu, Aihong Liu, Guangyuan Liu, Hong Yin, Guiquan Guan, Jianxun Luo
Theileria annulata is a tick-borne intracellular protozoan parasite that causes tropical theileriosis, a fatal bovine lymphoproliferative disease. The parasite predominantly invades bovine B lymphocytes and macrophages and induces host cell transformation by a mechanism that is not fully comprehended. Analysis of signaling pathways by quantitative real-time PCR (qPCR) could be a highly efficient means to understand this transformation mechanism. However, accurate analysis of qPCR data relies on selection of appropriate reference genes for normalization, yet few papers on T...
February 2016: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/26408597/evaluating-the-babesia-bovis-infection-of-cattle-in-china-with-enzyme-linked-immunosorbent-assay-elisa
#14
Congshan Yang, Junlong Liu, Anyan Li, Youquan Li, Aihong Liu, Junren Xie, Guangyuan Liu, Hong Yin, Guiquan Guan, Jianxun Luo
Babesia bovis is an important pathogen of bovine babesiosis and causes serious constraints on the health and productivity of domestic cattle in the tropical and subtropical areas of the world. Aiming to clarify the prevalence of B. bovis in China, a total of 2,364 cattle serum samples were randomly collected from 45 different areas of 17 provinces in China. Antibodies against B. bovis were tested by enzyme-linked immunosorbent assay (ELISA) using a recombinant C-terminal antigen of B. bovis rhoptry-associated protein-1 (RAP-1) to evaluate the prevalence of B...
December 2015: Acta Parasitologica
https://www.readbyqxmd.com/read/26026806/rhoptry-associated-protein-rap-1-genes-in-the-sheep-pathogen-babesia-sp-xinjiang-multiple-transcribed-copies-differing-by-3-end-repeated-sequences
#15
Qingli Niu, Jordan Marchand, Congshan Yang, Claire Bonsergent, Guiquan Guan, Hong Yin, Laurence Malandrin
Sheep babesiosis occurs mainly in tropical and subtropical areas. The sheep parasite Babesia sp. Xinjiang is widespread in China, and our goal is to characterize rap-1 (rhoptry-associated protein 1) gene diversity and expression as a first step of a long term goal aiming at developing a recombinant subunit vaccine. Seven different rap-1a genes were amplified in Babesia sp. Xinjiang, using degenerate primers designed from conserved motifs. Rap-1b and rap-1c gene types could not be identified. In all seven rap-1a genes, the 5' regions exhibited identical sequences over 936 nt, and the 3' regions differed at 28 positions over 147 nt, defining two types of genes designated α and β...
July 30, 2015: Veterinary Parasitology
https://www.readbyqxmd.com/read/26026059/prmt1-upregulated-by-epithelial-proinflammatory-cytokines-participates-in-cox2-expression-in-fibroblasts-and-chronic-antigen-induced-pulmonary-inflammation
#16
Qingzhu Sun, Li Liu, Michael Roth, Jia Tian, Qirui He, Bo Zhong, Ruanjuan Bao, Xi Lan, Congshan Jiang, Jian Sun, Xudong Yang, Shemin Lu
Protein arginine methyltransferase (PRMT)1, methylating both histones and key cellular proteins, has emerged as a key regulator of various cellular processes. This study aimed to identify the mechanism that regulates PRMT1 in chronic Ag-induced pulmonary inflammation (AIPI) in the E3 rat asthma model. E3 rats were challenged with OVA for 1 or 8 wk to induce acute or chronic AIPI. Expression of mRNAs was detected by real-time quantitative PCR. PRMT1, TGF-β, COX2, and vascular endothelial growth factor protein expression in lung tissues was determined by immunohistochemistry staining and Western blotting...
July 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/25785087/micrornas-associated-with-osteoarthritis-differently-expressed-in-bone-matrix-gelatin-bmg-rat-model
#17
Zixin Min, Rui Zhang, Jianfeng Yao, Congshan Jiang, Yuanxu Guo, Fei Cong, Wei Wang, Jia Tian, Nannan Zhong, Jian Sun, Jie Ma, Shemin Lu
Osteoarthritis (OA) is characterized by degeneration of articular cartilage, limited intraarticular inflammation with synovitis, and changes in peri-articular and subchondral bone. In recent years, more and more evidence demonstrated that microRNAs (miRNAs) play important roles in the molecular mechanisms in OA by suppressing gene expression at the post-transcriptional level. In current study, histological staining of toluidine blue and cartilage-specific gene express revealed that the bone matrix gelatin (BMG) rat model could demonstrate the different development of cartilage...
2015: International Journal of Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/25773183/in-vivo-and-in-vitro-efficacies-of-mebendazole-mefloquine-and-nitazoxanide-against-cyst-echinococcosis
#18
Congshan Liu, Haobing Zhang, Jianhai Yin, Wei Hu
Echinococcus granulosus is a cestode parasite. The metacestode stage causes cystic echinococcosis (CE) mainly in the human liver and lung. Current chemotherapy against CE is based on mebendazole and albendazole. However, benzimidazoles result in a low cure rate or are ineffective in many patients; therefore, novel compounds for the treatment of this disease have been studied. Mefloquine was reported to be dramatically effective on cultured Echinococcus multilocularis metacestodes in vitro. And, nitazoxanide has a prominent protoscolicidal effect...
June 2015: Parasitology Research
https://www.readbyqxmd.com/read/25730413/do-little-embryos-make-big-decisions-how-maternal-dietary-protein-restriction-can-permanently-change-an-embryo-s-potential-affecting-adult-health
#19
Tom P Fleming, Adam J Watkins, Congshan Sun, Miguel A Velazquez, Neil R Smyth, Judith J Eckert
Periconceptional environment may influence embryo development, ultimately affecting adult health. Here, we review the rodent model of maternal low-protein diet specifically during the preimplantation period (Emb-LPD) with normal nutrition during subsequent gestation and postnatally. This model, studied mainly in the mouse, leads to cardiovascular, metabolic and behavioural disease in adult offspring, with females more susceptible. We evaluate the sequence of events from diet administration that may lead to adult disease...
May 2015: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/25609498/epigenetic-regulation-of-histone-modifications-and-gata6-gene-expression-induced-by-maternal-diet-in-mouse-embryoid-bodies-in-a-model-of-developmental-programming
#20
Congshan Sun, Oleg Denisenko, Bhavwanti Sheth, Andy Cox, Emma S Lucas, Neil R Smyth, Tom P Fleming
BACKGROUND: Dietary interventions during pregnancy alter offspring fitness. We have shown mouse maternal low protein diet fed exclusively for the preimplantation period (Emb-LPD) before return to normal protein diet (NPD) for the rest of gestation, is sufficient to cause adult offspring cardiovascular and metabolic disease. Moreover, Emb-LPD blastocysts sense altered nutrition within the uterus and activate compensatory cellular responses including stimulated endocytosis within extra-embryonic trophectoderm and primitive endoderm (PE) lineages to protect fetal growth rate...
2015: BMC Developmental Biology
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