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gene expression in embryonic development

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https://www.readbyqxmd.com/read/29676825/histone-chaperones-play-crucial-roles-in-maintenance-of-stem-cell-niche-during-plant-root-development
#1
Jing Ma, Yuhao Liu, Wangbin Zhou, Yan Zhu, Aiwu Dong, Wen-Hui Shen
Stem cells in both plant and animal kingdoms reside in a specialized cellular context called stem-cell niche (SCN). SCN integrity is crucial for organism development. Here we show that the H3/H4 histone chaperone CHROMATIN ASSEMBLY FACTOR-1 (CAF-1) and the H2A/H2B histone chaperone NAP1-RELATED PROTEIN1/2 (NRP1/2) play synergistic roles in Arabidopsis root SCN maintenance. Compared to either the m56-1 double mutant deprived of NRP1 and NRP2 or the fas2-4 mutant deprived of CAF-1, the combined m56-1fas2-4 triple mutant displayed a much more severe short-root phenotype...
April 20, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29674317/the-atypical-mitogen-activated-protein-kinase-erk3-is-essential-for-establishment-of-epithelial-architecture
#2
Chika Takahashi, Koichi Miyatake, Morioh Kusakabe, Eisuke Nishida
Epithelia contribute to physical barriers that protect internal tissues from the external environment and also support organ structure. Accordingly, establishment and maintenance of epithelial architecture are essential for both embryonic development and adult physiology. Here, using gene knockout and -down techniques along with gene profiling, we show that extracellular signal-regulated kinase 3 (ERK3), a poorly characterized atypical mitogen-activated protein kinase (MAPK), regulates the epithelial architecture in vertebrates...
April 19, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29674165/identification-expression-and-function-of-myosin-heavy-chain-family-genes-in-tribolium-castaneum
#3
Chengjun Li, Juanjuan Liu, Peng Lü, Shangshang Ma, Keming Zhu, Lu Gao, Bin Li, Keping Chen
Functions of myosin heavy chain (myosin) family genes are poorly understood in some insects. To address this, we determined the expression and function of myosin family genes in Tribolium castaneum. TcMyo15 is predominantly expressed in early embryos, late larvae and early adults, but TcMyo7B transcripts significantly increased in late larvae. TcMyo20 transcripts are abundant in pre-adults, whereas TcMhc1 is highly expressed in post-embryonic stages. TcMhc2 shows peak expression in late pupae. TcMyo9 transcripts reached their highest levels in late pupae...
April 16, 2018: Genomics
https://www.readbyqxmd.com/read/29673042/regulation-of-neural-crest-development-by-the-formin-family-protein-daam1
#4
Olga Ossipova, Ryan Kerney, Jean-Pierre Saint-Jeannet, Sergei Y Sokol
The neural crest (NC) multipotent progenitor cells form at the neural plate border and migrate to diverse locations in the embryo to differentiate into many cell types. NC is specified by several embryonic pathways, however the role of noncanonical Wnt signaling in this process remains poorly defined. Daam1 is a formin family protein that is present in embryonic ectoderm at the time of NC formation and can mediate noncanonical Wnt signaling. Our interference experiments indicated that Daam1 is required for NC gene activation...
April 19, 2018: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/29672507/unconventional-function-of-an-achaete-scute-homolog-as-a-terminal-selector-of-nociceptive-neuron-identity
#5
Neda Masoudi, Saeed Tavazoie, Lori Glenwinkel, Leesun Ryu, Kyuhyung Kim, Oliver Hobert
Proneural genes are among the most early-acting genes in nervous system development, instructing blast cells to commit to a neuronal fate. Drosophila Atonal and Achaete-Scute complex (AS-C) genes, as well as their vertebrate orthologs, are basic helix-loop-helix (bHLH) transcription factors with such proneural activity. We show here that a C. elegans AS-C homolog, hlh-4, functions in a fundamentally different manner. In the embryonic, larval, and adult nervous systems, hlh-4 is expressed exclusively in a single nociceptive neuron class, ADL, and its expression in ADL is maintained via transcriptional autoregulation throughout the life of the animal...
April 2018: PLoS Biology
https://www.readbyqxmd.com/read/29668864/bovine-embryo-elongation-is-altered-due-to-maternal-fatty-acid-supplementation
#6
K Giller, B Drews, J Berard, H Kienberger, M Schmicke, J Frank, B Spanier, H Daniel, G Geisslinger, S E Ulbrich
The pre-implantation period is prone to embryonic losses in bovine. Embryo-maternal communication is crucial to support embryo development. Thereby, factors of the uterine fluid (UF) are of specific importance. The maternal diet can affect the UF composition. Since omega 3 fatty acids (omega 3 FA) are considered to be beneficial for reproduction, we investigated if dietary omega 3 FA affected factors in the UF related to embryo elongation. Angus heifers (n = 37) were supplemented with either 450 g of rumen-protected fish oil (omega 3 FA) or sunflower oil (omega 6 FA) for a period of 8 weeks...
April 12, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29667432/identification-and-characterisation-of-micrornas-and-piwi-interacting-rnas-in-cockerels-spermatozoa-by-solexa-sequencing
#7
Shengru Wu, Jiacheng Guo, Liqin Zhu, Jiantao Yang, Si Chen, Xiaojun Yang
1. There has been substantial research focused on the roles of microRNAs (miRNA) and PIWI-interacting RNAs (piRNA) delivered from mammalian spermatozoa, however comparatively little is known about the role of spermatozoa-delivered miRNAs and piRNAs within breeding cockerels' spermatozoa. 2. A small RNA library of cockerels' spermatozoa was constructed using Illumina high-throughput sequencing technology. Unique sequences with lengths of 18-26 nucleotides were mapped to miRBase 21.0 and unique sequences with lengths of 25-37 nucleotides were mapped to a piRNA database...
April 18, 2018: British Poultry Science
https://www.readbyqxmd.com/read/29666896/calpain-inhibition-attenuates-bleomycin-induced-pulmonary-fibrosis-via-switching-the-development-of-epithelial-mesenchymal-transition
#8
Yuan Liu, Bing Liu, Gu-Qin Zhang, Jing-Feng Zou, Meng-Lin Zou, Zhen-Shun Cheng
Calpains are intracellular calcium-dependent cysteine proteases, which cleave several substrates proteins, have been proven to play important roles in lung fibrosis. The aim of this study was to investigate the effects of calpain on bleomycin (BLM)-induced pulmonary fibrosis. A lung fibrosis mice model was established successfully by intraperitoneal injection of bleomycin. Calpeptin, a highly selective inhibitor of calpain activation, was administered three times weekly after bleomycin injection. Histological examination was used to assess the fibrosis...
April 18, 2018: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29666335/identification-molecular-characterization-and-analysis-of-the-expression-pattern-of-soxf-subgroup-genes-the-yellow-river-carp-cyprinus-carpio
#9
Tingting Liang, Yongfang Jia, Ruihua Zhang, Qiyan Du, Zhongjie Chang
Sox7, Sox17 and Sox18 are the members of the Sry-related high-mobility group box family (SoxF) of transcription factors. SoxF factors regulate endothelial cell fate as well as development and differentiation of blood cells and lymphatic vessels. There is very less information about the functions of these genes in fish. We obtained the full-length cDNA sequence of SoxF genes including Sox7, Sox17 and Sox18 in Cyprinus carpio, where Sox7 and Sox18 had two copies. The construction of a phylogenetic tree showed that these genes were homologous to the genes in other species...
March 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29666052/functional-comparison-of-distinct-brachyury-states-in-a-renal-differentiation-assay
#10
Jing Zhou, Antonius Plagge, Patricia Murray
Mesodermal populations can be generated in vitro from mouse embryonic stem cells (mESCs) using three-dimensional (3-D) aggregates called embryoid bodies or two-dimensional (2-D) monolayer culture systems. Here, we investigated whether Brachyury -expressing mesodermal cells generated using 3-D or 2-D culture systems are equivalent, or instead, have different properties. Using a Brachyury -GFP/E2-Crimson reporter mESC line, we isolated Brachyury- GFP+ mesoderm cells using flow-activated cell sorting and compared their gene expression profiles and ex vivo differentiation patterns...
April 17, 2018: Biology Open
https://www.readbyqxmd.com/read/29664137/msh-homeobox-1-polymorphisms-and-the-risk-of-non-syndromic-orofacial-clefts-a-meta-analysis
#11
Min Gu, Yan Zhang, Hualian Liu, Jue Liu, Danxia Zhu, Xu Yang
MSH homebox 1 (MSX1) is a susceptibility gene for non-syndromic orofacial clefts (NSOCs). Here, a meta-analysis was conducted to assess their associations. A systematic search of PubMed to 1 September 2017, was performed to retrieve all eligible studies. Odds ratios (ORs) were used to calculate the associations. The stability of the results was evaluated by sensitivity analysis. Publication bias was assessed using Begg's funnel plots and the Egger test. In silico Msx1 expression during early mouse craniofacial development was evaluated by the Gene Expression Omnibus...
April 17, 2018: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/29663529/a-group-of-tissue-specific-micrornas-contribute-to-the-silencing-of-cux1-in-different-cell-lineages-during-development
#12
Hui Xu, Jie-Hua He, Shi-Jun Xu, Shu-Juan Xie, Li-Ming Ma, Yin Zhang, Hui Zhou, Liang-Hu Qu
Cut-like homeobox 1 (CUX1) is a highly conserved homeoprotein that functions as a transcriptional repressor of genes specifying terminal differentiation. We previously showed that liver-specific microRNA-122 (miR-122) regulates the timing of liver development by silencing CUX1 post-transcriptionally. Since the CUX1 protein is expressed in a subset of embryonic tissues, we hypothesized that it is regulated by specific microRNAs (miRNAs) in each cell type during development. Using a large-scale screening method, we identified ten tissue-specific miRNAs from different cell lineages that directly targeted CUX1...
April 16, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29662628/a-gene-expression-signature-predicts-recurrence-free-survival-in-meningioma
#13
Adriana Olar, Lindsey D Goodman, Khalida M Wani, Nicholas S Boehling, Devi S Sharma, Reema R Mody, Joy Gumin, Elizabeth B Claus, Frederick F Lang, Timothy F Cloughesy, Albert Lai, Kenneth D Aldape, Franco DeMonte, Erik P Sulman
BACKGROUND: Meningioma is the most common primary brain tumor and has a variable risk of local recurrence. While World Health Organization (WHO) grade generally correlates with recurrence, there is substantial within-grade variation of recurrence risk. Current risk stratification does not accurately predict which patients are likely to benefit from adjuvant radiation therapy (RT). We hypothesized that tumors at risk for recurrence have unique gene expression profiles (GEP) that could better select patients for adjuvant RT...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29662623/cancer-reversion-with-oocyte-extracts-is-mediated-by-cell-cycle-arrest-and-induction-of-tumour-dormancy
#14
Norazalina Saad, Ramiro Alberio, Andrew D Johnson, Richard D Emes, Tom C Giles, Philip Clarke, Anna M Grabowska, Cinzia Allegrucci
Inducing stable control of tumour growth by tumour reversion is an alternative approach to cancer treatment when eradication of the disease cannot be achieved. The process requires re-establishment of normal control mechanisms that are lost in cancer cells so that abnormal proliferation can be halted. Embryonic environments can reset cellular programmes and we previously showed that axolotl oocyte extracts can reprogram breast cancer cells and reverse their tumorigenicity. In this study, we analysed the gene expression profiles of oocyte extract-treated tumour xenografts to show that tumour reprogramming involves cell cycle arrest and acquisition of a quiescent state...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29661911/patl2-is-a-key-actor-of-oocyte-maturation-whose-invalidation-causes-infertility-in-women-and-mice
#15
Marie Christou-Kent, Zine-Eddine Kherraf, Amir Amiri-Yekta, Emilie Le Blévec, Thomas Karaouzène, Béatrice Conne, Jessica Escoffier, Said Assou, Audrey Guttin, Emeline Lambert, Guillaume Martinez, Magalie Boguenet, Selima Fourati Ben Mustapha, Isabelle Cedrin Durnerin, Lazhar Halouani, Ouafi Marrakchi, Mounir Makni, Habib Latrous, Mahmoud Kharouf, Charles Coutton, Nicolas Thierry-Mieg, Serge Nef, Serge P Bottari, Raoudha Zouari, Jean Paul Issartel, Pierre F Ray, Christophe Arnoult
The genetic causes of oocyte meiotic deficiency (OMD), a form of primary infertility characterised by the production of immature oocytes, remain largely unexplored. Using whole exome sequencing, we found that 26% of a cohort of 23 subjects with OMD harboured the same homozygous nonsense pathogenic mutation in PATL2 , a gene encoding a putative RNA-binding protein. Using Patl2 knockout mice, we confirmed that PATL2 deficiency disturbs oocyte maturation, since oocytes and zygotes exhibit morphological and developmental defects, respectively...
April 16, 2018: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/29661797/fank1-and-jazf1-promote-multiciliated-cell-differentiation-in-the-mouse-airway-epithelium
#16
Jo-Anne Johnson, Julie K Watson, Marko Z Nikolić, Emma L Rawlins
The airways are lined by secretory and multiciliated cells which function together to remove particles and debris from the respiratory tract. The transcriptome of multiciliated cells has been extensively studied, but the function of many of the genes identified is unknown. We have established an assay to test the ability of over-expressed transcripts to promote multiciliated cell differentiation in mouse embryonic tracheal explants. Overexpression data indicated that Fibronectin type 3 and ankyrin repeat domains 1 ( Fank1 ) and JAZF zinc finger 1 ( Jazf1 ) promoted multiciliated cell differentiation alone, and cooperatively with the canonical multiciliated cell transcription factor Foxj1...
April 16, 2018: Biology Open
https://www.readbyqxmd.com/read/29660519/oocyte-maturation-and-origin-of-the-germline-as-revealed-by-the-expression-of-nanos-like-in-the-pacific-oyster-crassostrea-gigas
#17
Rui Xu, Qi Li, Hong Yu, Lingfeng Kong
Nanos gene plays an important role in germline development in animals. However, the molecular mechanisms involved in germline development in Mollusca, the second largest animal phylum, are still poorly understood. Here we identified the Nanos orthologue from the Pacific oyster Crassostrea gigas (Cg-Nanos-like), and investigated the expression patterns of Nanos during gametogenesis and embryogenesis in C. gigas. Tissue expression analysis showed that Cg-Nanos-like was specifically expressed in female gonads...
April 13, 2018: Gene
https://www.readbyqxmd.com/read/29659814/the-first-whole-transcriptomic-exploration-of-pre-oviposited-early-chicken-embryos-using-single-and-bulked-embryonic-rna-sequencing
#18
Young Sun Hwang, Minseok Seo, Hee Jung Choi, Sang Kyung Kim, Heebal Kim, Jae Yong Han
Background: The chicken is a valuable model organism, especially in evolutionary and embryology research because its embryonic development occurs in the egg. However, despite its scientific importance, no transcriptome data have been generated for deciphering the early developmental stages of the chicken because of practical and technical constraints in accessing pre-oviposited embryos. Findings: Here, we determine the entire transcriptome of pre-oviposited avian embryos, including oocyte, zygote, and intrauterine embryos from Eyal-giladi and Kochav stage I (EGK...
April 1, 2018: GigaScience
https://www.readbyqxmd.com/read/29658437/decreased-expression-of-hoxa10-may-activate-the-autophagic-process-in-ovarian-endometriosis
#19
Jingjie Zheng, Xishao Luo, Jiaping Bao, Xiaowang Huang, Yi Jin, Lin Chen, Feiyun Zheng
Autophagy is a survival process that maintains homeostasis in all eukaryotic cells. Recent studies show an abnormal autophagic activity in endometriosis, but the role of autophagy is controversial. Homeobox A10 (HOXA10) is a transcription factor necessary for embryonic and adult uterine development, and studies indicate that its expression decreases in endometriosis. Homeobox A10 may negatively regulate autophagy in endometriosis. To test this hypothesis, we measured the expression levels of autophagic biomarkers (beclin-1 and LC3-II) and HOXA10 proteins by Western blotting and messenger RNA (mRNA) by quantitative real-time polymerase chain reaction...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29657129/multi-stage-differentiation-defines-melanoma-subtypes-with-differential-vulnerability-to-drug-induced-iron-dependent-oxidative-stress
#20
Jennifer Tsoi, Lidia Robert, Kim Paraiso, Carlos Galvan, Katherine M Sheu, Johnson Lay, Deborah J L Wong, Mohammad Atefi, Roksana Shirazi, Xiaoyan Wang, Daniel Braas, Catherine S Grasso, Nicolaos Palaskas, Antoni Ribas, Thomas G Graeber
Malignant transformation can result in melanoma cells that resemble different stages of their embryonic development. Our gene expression analysis of human melanoma cell lines and patient tumors revealed that melanoma follows a two-dimensional differentiation trajectory that can be subclassified into four progressive subtypes. This differentiation model is associated with subtype-specific sensitivity to iron-dependent oxidative stress and cell death known as ferroptosis. Receptor tyrosine kinase-mediated resistance to mitogen-activated protein kinase targeted therapies and activation of the inflammatory signaling associated with immune therapy involves transitions along this differentiation trajectory, which lead to increased sensitivity to ferroptosis...
April 3, 2018: Cancer Cell
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