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https://www.readbyqxmd.com/read/28324679/methicillin-resistant-and-susceptible-staphylococcus-aureus-from-retail-meat-in-denmark
#1
Yuanyue Tang, Jesper Larsen, Jette Kjeldgaard, Paal Skytt Andersen, Robert Skov, Hanne Ingmer
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is increasingly related to human infections. Farmers and veterinarians have the highest risk, but infections have also occurred in individuals without prior contact to livestock. Clonal complex (CC) 398 is the predominant LA-MRSA lineage causing human infections, and although pigs are the major source of CC398 worldwide, poultry and other animals are also reservoirs. This raises concern for transmission of MRSA via meat. In this study, the occurrence and characteristics of S...
March 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28321991/atomic-layer-deposition-of-a-silver-nanolayer-on-advanced-titanium-orthopedic-implants-inhibits-bacterial-colonization-and-supports-vascularized-de-novo-bone-ingrowth
#2
Aine Devlin-Mullin, Naomi M Todd, Zahra Golrokhi, Hua Geng, Moritz A Konerding, Nigel G Ternan, John A Hunt, Richard J Potter, Chris Sutcliffe, Eric Jones, Peter D Lee, Christopher A Mitchell
Joint replacement surgery is associated with significant morbidity and mortality following infection with either methicillin-resistant Staphylococcus aureus (MRSA) or Staphylococcus epidermidis. These organisms have strong biofilm-forming capability in deep wounds and on prosthetic surfaces, with 10(3) -10(4) microbes resulting in clinically significant infections. To inhibit biofilm formation, we developed 3D titanium structures using selective laser melting and then coated them with a silver nanolayer using atomic layer deposition...
March 21, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28321262/allium-stipitatum-extract-exhibits-in-vivo-antibacterial-activity-against-methicillin-resistant-staphylococcus-aureus-and-accelerates-burn-wound-healing-in-a-full-thickness-murine-burn-model
#3
Arunkumar Karunanidhi, Ehsanollah Ghaznavi-Rad, Jayakayatri Jeevajothi Nathan, Yusuf Abba, Alex van Belkum, Vasanthakumari Neela
The in vivo antibacterial and burn wound healing potency of Persian shallot bulbs (Allium stipitatum) were explored in a mice burn model infected with methicillin-resistant Staphylococcus aureus (MRSA). Hexane (ASHE) and dichloromethane (ASDE) extracts were tested. Female BALB/c mice were inflicted with third-degree thermal injury followed by infection with MRSA. ASHE and ASDE formulated with simple ointment base (SOB) at concentrations of 1%, 2%, and 5% (w/w) were topically applied to burn wounds twice a day for 20 days...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28321210/production-of-ramoplanin-and-ramoplanin-analogs-by-actinomycetes
#4
Mercedes de la Cruz, Ignacio González, Craig A Parish, Russell Onishi, José R Tormo, Jesús Martín, Fernando Peláez, Debbie Zink, Noureddine El Aouad, Fernando Reyes, Olga Genilloud, Francisca Vicente
Ramoplanin is a glycolipodepsipeptide antibiotic obtained from fermentation of Actinoplanes sp. ATCC 33076 that exhibits activity against clinically important multi-drug-resistant, Gram-positive pathogens including vancomycin-resistant Enterococcus (VRE), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-intermediate resistant Clostridium difficile. It disrupts bacterial cell wall through a unique mechanism of action by sequestering the peptidoglycan intermediate Lipid II and therefore does not show cross-resistance with other antibiotics...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28320357/outcome-of-neurological-early-rehabilitation-patients-carrying-multi-drug-resistant-bacteria-results-from-a-german-multi-center-study
#5
J D Rollnik, M Bertram, C Bucka, M Hartwich, M Jöbges, G Ketter, B Leineweber, M Mertl-Rötzer, D A Nowak, T Platz, K Scheidtmann, R Thomas, F von Rosen, C W Wallesch, H Woldag, P Peschel, J Mehrholz, M Pohl
BACKGROUND: Colonization or infection with multi-drug resistant (MDR) bacteria is considered detrimental to the outcome of neurological and neurosurgical early rehabilitation patients. METHODS: In a German multi-center study, 754 neurological early rehabilitation patients were enrolled and and reviewed in respect to MDR status, length of stay (LOS) and the following outcome variables: Barthel Index (BI), Early Rehabilitation Index (ERI), Glasgow Outcome Score Extended (GOSE), Coma Remission Scale (CRS), Functional Ambulation Categories (FAC)...
March 20, 2017: BMC Neurology
https://www.readbyqxmd.com/read/28319829/evaluation-of-the-pharmacokinetics-of-imipenem-following-regional-limb-perfusion-using-the-saphenous-and-the-cephalic-veins-in-standing-horses
#6
G Kelmer, A J Tatz, E Kdoshim, M Britzi, G Segev
This prospective experimental study goal was to determine the pharmacokinetics of imipenem after intravenous regional limb perfusion (IV-RLP) in standing horses. Nine horses participated in the study; that was approved by the University Animal Care and Use Committee. One thoracic limb or one pelvic limb of each horse was randomly selected. After the veins were catheterized, an Esmarch bandage tourniquet was applied and the catheter was injected with a solution containing 500mg of imipenem. Synovial fluid samples were collected from the fetlock joint and blood samples were collected from the jugular vein...
February 24, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28316313/flank-hernia-repair-with-suture-anchor-mesh-fixation-to-the-iliac-crest
#7
Kaushik Mukherjee, Richard S Miller
Traumatic or postsurgical flank hernias are complex and prone to recurrence, particularly at the border of the iliac crest. We reviewed our experience using suture anchors drilled into the iliac crest to fixate the mesh to bone. Our study of 10 repairs in eight patients was Institutional Review Board exempt. We obtained demographics, body mass index, diabetes, methicillin-resistant Staphylococcus aureus (MRSA) history, smoking status, steroid use, number of prior repairs, defect size, mesh size, number of anchors, and recurrence and infection at follow-up...
March 1, 2017: American Surgeon
https://www.readbyqxmd.com/read/28315144/monitoring-antimicrobial-resistance-amr-using-cusum-control-charts
#8
L Righi, R Amarsy, M-Q Picat, M Thuillier, E Cambau, L Raskine, S Chevret, R Flicoteaux
We evaluated the use of the Cumulative Summation (CUSUM) control chart methodology for detection of an excessive increase in antimicrobial-resistant (AMR) bacteria acquisition. We used administrative, clinical and bacteriological data from all 157,570 patients hospitalized for at least 48 h from January 1, 2010 to December 31, 2015 in a 654-bed university teaching hospital in Paris, France. Monthly computed CUSUM were evaluated for the detection of out-of-control situations, defined as incidence rates of acquired AMR bacterial colonization exceeding acceptable thresholds at the hospital and ward levels (based on six selected wards) for AMR bacteria overall and Extended-spectrum beta-lactamases Enterobacteriaceae (ESBL-E) and Methicillin-resistant Staphylococcus aureus (MRSA), specifically...
March 17, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28314997/clinical-and-microbiological-outcomes-in-haematogenous-spondylodiscitis-treated-conservatively
#9
Tiziana Ascione, Giovanni Balato, Sigismondo Luca Di Donato, Pasquale Pagliano, Francesco Granata, Gianluca Colella, Carlo Ruosi
INTRODUCTION: Spondylodiscitis refers to infections of the intervertebral disc and the adjacent vertebral body. Although it is still considered a rare condition, its rate is projected to increase. Mortality rate is considered to be low, but an estimated one third of the survivors experience residual disabilities. Literature shows that uncomplicated spondylodiscitis can be adequately treated by early antibiotic therapy and immobilization. The aim of the study is to evaluate the outcome of conservative treatment in patients with haematogenous spondylodiscitis...
March 17, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28304109/methicillin-resistant-staphylococcus-aureus-mrsa-a-controversial-food-borne-pathogen
#10
REVIEW
Daniil Sergelidis, Apostolos S Angelidis
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of severe healthcare-associated (HA) infections. Although during the last decade the incidence of HA invasive infections has dropped, the incidence of community-associated MRSA (CA-MRSA) infections has risen among the general population. Moreover, CA-MRSA, livestock-associated MRSA (LA-MRSA) and HA MRSA (HA-MRSA) can be found in foods intended for human consumption. Several studies from different geographical areas have reported the presence of enterotoxin genes in several MRSA food isolates...
March 17, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28298453/detection-of-mecc-positive-staphylococcus-aureus-what-to-expect-from-immunological-tests-targeting-pbp2a
#11
Céline Dupieux, Coralie Bouchiat, Anders Rhod Larsen, Bruno Pichon, Mark Holmes, Christopher Teale, Giles Edwards, Robert Hill, Jean-Winoc Decousser, Sophie Trouillet-Assant, Andreas Petersen, Robert Skov, Angela Kearns, Frédéric Laurent
Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is pivotal for the control of their dissemination and for accurate management of infected patients.….
March 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28296768/difficult-clinical-management-of-antituberculosis-dress-syndrome-complicated-by-mrsa-infection-a-case-report
#12
Li Wang, Lin-Feng Li
RATIONALE: Drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome is a severe drug-induced hypersensitivity reaction characterized by skin rash, fever, blood abnormalities, and multiple organ involvement. The diagnosis of DRESS syndrome is often delayed because of its variable presentation. Prompt withdrawal of the culprit drug is the definitive treatment. DRESS syndrome induced by antituberculosis drugs has rarely been reported. PATIENT CONCERNS: A 50-year-old man admitted to our hospital with recurrent episodes of progressive rash, fever, eosinophilia, lymphadenopathy, hepatic, and pulmonary involvement were experienced after repeat trials of the same antituberculosis drugs...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28293964/dosing-strategies-to-optimize-currently-available-anti-mrsa-treatment-options-part-1-iv-options
#13
Ronald G Hall, Michael Thatcher, Wei Wei, Shibin Varghese, Lincy Varughese, Michelle Ndiulor, Kenna D Payne
Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a predominant pathogen resulting in significant morbidity and mortality. Optimal dosing of anti-MRSA agents is needed to help prevent the development of antimicrobial resistance and to increase the likelihood of a favorable clinical outcome. Areas covered: This review summarizes the available data for antimicrobials routinely used for MRSA infections that are not administered orally or topically. We make recommendations and highlight the current gaps in the literature...
March 15, 2017: Expert Review of Clinical Pharmacology
https://www.readbyqxmd.com/read/28293153/susceptibility-profile-of-methicillin-resistant-staphylococcus-aureus-to-linezolid-in-clinical-isolates
#14
Ali Shariq, Syed Bilal Tanvir, Atif Zaman, Salman Khan, Armeena Anis, Misha Aftab Khan, Sumaira Ahmed
OBJECTIVE: To determine the resistance and sensitivity pattern of methicillin-resistant Staphylococcus aureus (MRSA) isolates to linezolid (LZD) along with its prevalence in a tertiary care hospital of Karachi, Pakistan. MATERIALS AND METHODS: A cross-sectional study was carried out. This study lasted for about 1 year. Prevalence and sensitivity of LZD, vancomycin, and oxacillin was tested against isolates of MRSA. RESULTS: Out of total 369 specimens 165 were found to be MRSA making the prevalence in our study 44...
January 2017: International Journal of Health Sciences
https://www.readbyqxmd.com/read/28290363/volatile-and-non-volatile-semi-volatile-compounds-and-in-vitro-bioactive-properties-of-chilean-ulmo-eucryphia-cordifolia-cav-honey
#15
Francisca Acevedo, Paulina Torres, B Dave Oomah, Severino Matias de Alencar, Adna Prado Massarioli, Raquel Martín-Venegas, Vicenta Albarral-Ávila, César Burgos-Díaz, Ruth Ferrer, Mónica Rubilar
Ulmo honey originating from Eucryphia cordifolia tree, known locally in the Araucania region as the Ulmo tree is a natural product with valuable nutritional and medicinal qualities. It has been used in the Mapuche culture to treat infections. This study aimed to identify the volatile and non-volatile/semi-volatile compounds of Ulmo honey and elucidate its in vitro biological properties by evaluating its antioxidant, antibacterial, antiproliferative and hemolytic properties and cytotoxicity in Caco-2 cells. Headspace volatiles of Ulmo honey were isolated by solid-phase microextraction (SPME); non-volatiles/semi-volatiles were obtained by removing all saccharides with acidified water and the compounds were identified by GC/MS analysis...
April 2017: Food Research International
https://www.readbyqxmd.com/read/28289460/the-evaluation-of-mrsa-surveillance-cultures-by-the-number-and-combinations-of-anatomical-sites
#16
Irena Grmek Kosnik, Alenka Storman, Ziva Petrovic, Slavica Robnik, Urska Dermota, Tjasa Zohar Cretnik
INTRODUCTION: The identification of patients infected and/or colonised by methicillin resistant Staphylococcus aureus (MRSA) is necessary for the timely introduction of measures for infection control. We compared the diagnostic efficacy of combinations of MRSA surveillance swabs routinely taken by health institutions in the country. METHODS: All surveillance samples, which were sent for a microbiological analysis to detect MRSA with the culture method in 2014, in the three departments for medical microbiology of the National Laboratory for Health, Environment and Food, were included in this study...
March 1, 2017: Zdravstveno Varstvo
https://www.readbyqxmd.com/read/28289036/characterizing-the-antimicrobial-activity-of-n-2-n-4-disubstituted-quinazoline-2-4-diamines-towards-multidrug-resistant-acinetobacter-baumannii
#17
Renee Fleeman, Kurt S Van Horn, Megan M Barber, Whittney N Burda, David L Flanigan, Roman Manetsch, Lindsey N Shaw
We previously reported a series of N(2) ,N(4) -disubstituted quinazoline-2,4-diamine as dihydrofolate reductase inhibitors, with potent in vitro and in vivo antibacterial activity against MRSA strains. In this work we extend our previous study to the Gram-negative pathogen Acinetobacter baumannii We determined that optimized N(2) ,N(4) -disubstituted quinazoline-2,4-diamines are strongly antibacterial against multi-drug resistant A. baumannii strains when the 6-position is substituted with a halide or an alkyl substituent...
March 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28284111/characteristics-and-predictors-of-outcome-of-spontaneous-spinal-epidural-abscesses-treated-conservatively-a-retrospective-cohort-study-in-a-referral-center
#18
Nikolaos Spernovasilis, Stelios Demetriou, Maria Bachlitzanaki, Ioannis Gialamas, Kalliopi Alpantaki, Georgios Hamilos, Apostolos Karantanas, Achilleas Gikas
OBJECTIVE: Recent studies have shown that in carefully selected patients, conservative treatment alone can be an option in the management of spinal epidural abscess (SEA). The aim of this study was to identify prognostic factors of outcome in patients with spontaneous SEA treated conservatively. PATIENTS AND METHODS: A retrospective cohort study of all patients with spontaneous SEA treated with antibiotics alone from January 2012 to December 2015 was conducted in a 1200-bed tertiary referral center...
February 27, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28283006/-investigation-of-biofilm-formation-properties-of-staphylococcus-isolates
#19
Duygu Nilüfer Öcal, İştar Dolapçı, Zeynep Ceren Karahan, Alper Tekeli
Biofilm production is an important virulence factor which allows staphylococci to adhere to medical devices. The principal component of biofilm is a "polysaccharide intercellular adhesin (PIA)" which is composed of a beta-1,6-N-acetylglucosamine polymer synthesized by an enzyme (N-acetylglucosamine transferase) encoded by the ica operon found on the bacterial chromosome. This operon is composed of four genes (A, B, C, and D), and a transposable element IS256. In this study, we aimed to determine the biofilm production characteristics of invasive/non-invasive staphylococcus isolates and different staphylococcus species...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28270804/eradication-and-sensitization-of-methicillin-resistant-staphylococcus-aureus-to-methicillin-with-bioactive-extracts-of-berry-pomace
#20
Serajus Salaheen, Mengfei Peng, Jungsoo Joo, Hironori Teramoto, Debabrata Biswas
The therapeutic roles of phenolic blueberry (Vaccinium corymbosum) and blackberry (Rubus fruticosus) pomace (commercial byproduct) extracts (BPE) and their mechanism of actions were evaluated against methicillin resistant Staphylococcus aureus (MRSA). Five major phenolic acids of BPE, e.g., protocatechuic, p. coumaric, vanillic, caffeic, and gallic acids, as well as crude BPE completely inhibited the growth of vegetative MRSA in vitro while BPE+methicillin significantly reduced MRSA biofilm formation on plastic surface...
2017: Frontiers in Microbiology
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