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https://www.readbyqxmd.com/read/28431450/-livestock-associated-methicillin-resistant-staphylococcus-aureus-epidemiological-data-from-an-orthopaedic-department-in-north-west-germany
#1
Gerrit Steffen Maier, Fritz Thorey, Kristina Kolbow, Djordje Lazovic, Markus Lühmann, Jörg Ohnsorge, Uwe Maus
Background MRSA represents a considerable health threat to orthopaedic patients. Throughout the last decade, livestock-associated MRSA emerged increasingly in livestock all over Europe. LA-MRSA broke the species barrier, colonizing and causing infections in humans. Nevertheless, to date there are no studies concerning livestock-associated MRSA and orthopaedic patients. Therefore, we aimed to investigate the prevalence of LA-MRSA in a regional orthopaedic department localized in an area with high-density livestock...
April 21, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/28430811/antimicrobial-resistance-in-coagulase-positive-staphylococci-isolated-from-companion-animals-in-australia-a-one-year-study
#2
Sugiyono Saputra, David Jordan, Kate A Worthing, Jacqueline M Norris, Hui S Wong, Rebecca Abraham, Darren J Trott, Sam Abraham
Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nation-wide survey on antimicrobial resistance in animal pathogens in Australia for a one-year period (January 2013 to January 2014)...
2017: PloS One
https://www.readbyqxmd.com/read/28428342/lysibodies-are-igg-fc-fusions-with-lysin-binding-domains-targeting-staphylococcus-aureus-wall-carbohydrates-for-effective-phagocytosis
#3
Assaf Raz, Anna Serrano, Christine Lawson, Maneesha Thaker, Tricia Alston, Stylianos Bournazos, Jeffrey V Ravetch, Vincent A Fischetti
The cell wall of Gram-positive bacteria contains abundant surface-exposed carbohydrate molecules that are highly conserved within and often across species. The potential therapeutic usefulness of high-affinity antibodies to cell wall carbohydrates is unquestioned, however obtaining such antibodies is challenging due to the poor overall immunogenicity of these bacterial targets. Autolysins and phage lysins are peptidoglycan hydrolases, enzymes that have evolved over a billion years to degrade bacterial cell wall...
April 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28424995/the-emergence-of-vancomycin-resistant-staphylococcus-aureus-in-an-intensive-care-unit-in-kerman-iran
#4
Yasser Fasihi, Fereshteh Saffari, Shahla Mansouri, Davood Kalantar-Neyestanaki
Methicillin-resistant Staphylococcus aureus (MRSA) is a global threat to public health. This study is the first report of the emergence of vancomycin-resistant MRSA in Kerman, Iran. During a period of 15 months, a total of 205 clinical isolates of S. aureus were collected from three university hospitals affiliated with the Kerman University of Medical Science, Kerman, Iran. Screening of methicillin and vancomycin resistance was carried out by phenotypic methods. The resistance and virulence genes of vancomycin-resistant isolates were detected by polymerase chain reaction (PCR)...
April 19, 2017: Wiener Medizinische Wochenschrift
https://www.readbyqxmd.com/read/28423435/-pathogen-spectrum-of-complex-wounds-in-germany-from-war-zones
#5
Hans Joachim Riesner, Benedikt Friemert, Patricia Lang, Hans Georg Palm, Arnold J Suda
Background Treatment of the injured from war zones with wounds with multi-resistant pathogens is becoming more common in Germany, especially in German Armed Forces Hospitals. In most cases, the pre-treatment of these patients has been inadequate and they have a high load of rare pathogens. In Germany, hospitals have to evaluate these pathogens by law. The aim of this study was to review current German guidelines and data on the array of pathogens of complex trauma wounds in Germany and to compare patients with wounds and multi-resistant pathogens from war zones treated in Germany...
April 19, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/28422484/direct-synthesis-of-dextran-based-antibacterial-hydrogels-for-extended-release-of-biocides-and-eradication-of-topical-biofilms
#6
Jiaul Hoque, Jayanta Haldar
Cationic small molecular biocides have been developed as promising antibiofilm agents because of their tunability in chemical structures and ability to disrupt established biofilms. However, the impact of biocides in antibiofilm treatment is largely limited due to lack of an effective delivery system that can ensure sustained release of biocide at the target site. Herein we report biocide-encapsulated antibacterial and antibiofilm hydrogel that acts as efficient delivery vehicle for the biocide and eradicates matured bacterial biofilm...
April 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28422471/-epidemiological-surveillance-for-multidrug-resistant-microorganisms-in-a-general-icu
#7
A Fernández-Verdugo, J Fernández, D Escudero, L Cofiño, L Forcelledo, M Telenti, E García-Prieto, R Rodríguez-García, L Álvarez-García, A Pérez-García, C Rodríguez-Lucas, F Vazquez
OBJECTIVE: Multidrug resistant (MDR) microorganisms represent a threat for patients admitted in Intensive Care Units (ICUs). The objective of the present study is to analyse the results of epidemiological surveillance cultures for these microorganisms in one of these units. METHODS: General ICU. Retrospective analysis, descriptive statistics. Analysis of epidemiological surveillance cultures for MDR microorganisms in 2015. Studied microorganisms: Methicillin-resistant Staphylococcus aureus (MRSA), ESBL-and/or carbapenemase-producing Klebsiella pneumoniae (CESBL-KP) and MDR Acinetobacter baumannii (MDRAB)...
April 5, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28421046/horses-in-denmark-are-a-reservoir-of-diverse-clones-of-methicillin-resistant-and-susceptible-staphylococcus-aureus
#8
Md Zohorul Islam, Carmen Espinosa-Gongora, Peter Damborg, Raphael N Sieber, Rikke Munk, Louise Husted, Arshnee Moodley, Robert Skov, Jesper Larsen, Luca Guardabassi
Denmark is a country with high prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 in pigs. Even though pig farming is regarded as the main source of human infection or colonization with MRSA CC398, 10-15% of the human cases appear not to be linked to pigs. Following the recent reports of MRSA CC398 in horses in other European countries and the lack of knowledge on S. aureus carriage in this animal species, we carried out a study to investigate whether horses constitute a reservoir of MRSA CC398 in Denmark, and to gain knowledge on the frequency and genetic diversity of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28419870/methicillin-resistant-staphylococcus-aureus-nasal-colonisation-amongst-healthcare-workers-in-kurdistan-region-iraq
#9
Nawfal R Hussein, Mahde S Assafi, Tayyaba Ijaz
OBJECTIVES: The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) compared with non-HCWs at Duhok city, Kurdistan Region, northern Iraq. METHODS: A total of 182 HCWs with different occupations and working in different hospital units as well as 198 non-HCWs were recruited. Nasal swab samples were collected and were inoculated on mannitol salt agar and incubated at 35°C for 48h...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28419202/genomic-characterization-of-usa300-mrsa-to-evaluate-intraclass-transmission-and-recurrence-of-ssti-among-high-risk-military-trainees
#10
Eugene V Millar, Gregory K Rice, Emad M Elassal, Carey D Schlett, Jason W Bennett, Cassie L Redden, Deepika Mor, Natasha N Law, David R Tribble, Theron Hamilton, Michael W Ellis, Kimberly A Bishop-Lilly
Background.: Military trainees are at increased risk for methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infection (SSTI). Whole genome sequencing (WGS) can refine our understanding of MRSA transmission and microevolution in congregate settings. Methods.: We conducted a prospective case-control study of SSTI among US Army Infantry trainees at Fort Benning, GA from July 2012-December 2014. We identified clusters of USA300 MRSA SSTI within select training classes and performed WGS on clinical isolates...
April 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28419033/oral-only-linezolid-rifampin-is-highly-effective-compared-with-other-antibiotics-for-periprosthetic-joint-infection-study-of-a-mouse-model
#11
John M Thompson, Vikram Saini, Alyssa G Ashbaugh, Robert J Miller, Alvaro A Ordonez, Roger V Ortines, Yu Wang, Robert S Sterling, Sanjay K Jain, Lloyd S Miller
BACKGROUND: The medical treatment of periprosthetic joint infection (PJI) involves prolonged systemic antibiotic courses, often with suboptimal clinical outcomes including increased morbidity and health-care costs. Oral and intravenous monotherapies and combination antibiotic regimens were evaluated in a mouse model of methicillin-resistant Staphylococcus aureus (MRSA) PJI. METHODS: Oral linezolid with or without oral rifampin, intravenous vancomycin with oral rifampin, intravenous daptomycin or ceftaroline with or without oral rifampin, oral doxycycline, or sham treatment were administered at human-exposure doses for 6 weeks in a mouse model of PJI...
April 19, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28417109/intractable-methicillin-resistant-staphylococcus-aureus-otorrhea-with-silicone-impression-in-the-middle-ear
#12
Eun Kyung Jung, Chul Ho Jang, Jong Yuap Seong, Yong Beom Cho
We report a case of methicillin-resistant Staphylococcus aureus (MRSA) otorrhea with impression material of hearing aid in the middle ear. The patient had chronic otitis media in the right ear with sensorineural hearing loss in both ears. The silicone flowed into the middle ear through a tympanic membrane perforation during the process of making an ear mold. Several days after hearing aid fitting, the patient had severe otalgia, intractable otorrhea, aggravated hearing loss, and dizziness. The pus culture and sensitivity test revealed MRSA...
April 2017: Journal of Audiology & Otology
https://www.readbyqxmd.com/read/28416961/predictive-factors-of-methicillin-resistant-staphylococcus-aureus-infection-in-elderly-patients-with-community-onset-pneumonia
#13
Hyeyoung Jwa, Jong Wook Beom, Jong Hoo Lee
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) infection is a severe and life-threatening disease in patients with community-onset (CO) pneumonia. However, the current guidelines lack specificity for a screening test for MRSA infection. METHODS: This study was retrospectively conducted in elderly patients aged ≥65 years, who had contracted CO-pneumonia during hospitalization at the Jeju National University Hospital, between January 2012 and December 2014...
April 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28413476/the-mechanism-of-human-%C3%AE-defensin-3-in-mrsa-induced-infection-of-implant-drug-resistant-bacteria-biofilm-in-the-mouse-tibial-bone-marrow
#14
Chen Zhu, Ni-Rong Bao, Shuo Chen, Jian-Ning Zhao
The mechanism of human β-defensin 3 (HBD-3) in methicillin-resistant Staphylococcus aureus (MRSA-induced infection of implant drug-resistant bacteria biofilm in the mouse tibial bone marrow was studied. Healthy adult male Sprague-Dawley rats with average weight of 230 g were selected to construct the infection model of MRSA-induced implant drug-resistant bacteria biofilm in the mouse left tibial bone marrow. The drugs were intraperitoneally injected after 24 h medullary cavity infection, and the experimental groups included the model group, HBD-3 group, and vancomycin group (20 rats in each group)...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28412663/a-facile-rapid-and-sensitive-detection-of-mrsa-using-a-crispr-mediated-dna-fish-method-antibody-like-dcas9-sgrna-complex
#15
Kyeonghye Guk, Joo Oak Keem, Seul Gee Hwang, Hyeran Kim, Taejoon Kang, Eun-Kyung Lim, Juyeon Jung
Rapid and reliable diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) is crucial for guiding effective patient treatment and preventing the spread of MRSA infections. Nonetheless, further simplification of MRSA detection procedures to shorten detection time and reduce labor relative to that of conventional methods remains a challenge. Here, we have demonstrated a Clustered regularly interspaced palindromic repeats (CRISPR)-mediated DNA-FISH method for the simple, rapid and highly sensitive detection of MRSA; this method uses CRISPR associated protein 9/single-guide RNA (dCas9/sgRNA) complex as a targeting material and SYBR Green I (SG I) as a fluorescent probe...
April 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28412131/screening-nasal-swabs-for-methicillin-resistant-staphylococcus-aureus-a-regional-burn-center-s-experience
#16
Shawn Tejiram, Laura S Johnson, Mihriye Mete, Sameer Desale, Kimberly Johnson, Jenny Zhang, Lauren T Moffatt, Jeffrey W Shupp
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen that can result in substantial morbidity and mortality. Early detection of MRSA colonization by screening nasal swabs may be important in the management of burn-injured patients. However, studies examining its use in this population are limited. The aim of this study was to study the utility of admission MRSA screening nasal swabs and determine if being positive for MRSA on admission impacted outcomes. MATERIALS AND METHODS: A retrospective review was conducted of burn patients who presented to a single regional burn center between June 2012 and December 2014...
April 12, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28410834/wearing-id-badges-in-the-operating-room-environment-is-reconsideration-warranted
#17
Matthew H Hogue, Kris P Heilmann, John J Callaghan
BACKGROUND: Surgical site infection and nosocomial infections in general have appropriately undergone increased scrutiny over the last decade. Numerous studies have documented pathogenic bacterial contamination of personal items such as cell phones, pagers, ties, and pens in the hospital setting. It is our understanding that Joint Commission on Accreditation of Healthcare Organizations requires all personnel to wear an identification badge at all times, which includes the operating room environment...
February 3, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28409124/hospital-dissemination-of-tst-1-positive-clonal-complex-5-cc5-methicillin-resistant-staphylococcus-aureus
#18
Min Wang, Yi Zheng, Jose R Mediavilla, Liang Chen, Barry N Kreiswirth, Yajun Song, Ruifu Yang, Hong Du
Methicillin-resistant Staphylococcus aureus (MRSA), is one of the most prevalent clinical pathogens isolated from hospital settings, and has increasingly identified in community settings. In China, the SCCmecIII-ST239 strains are disseminated in different geographic regions, accounting for >75% of all MRSA isolates in some national studies. Here we characterized 150 non-duplicate MRSA isolates collected from February 2012 to May 2013 in a tertiary hospital in Suzhou, Eastern China, to explore the molecular epidemiology...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28407796/prevalence-of-methicillin-resistant-staphylococcus-aureus-mrsa-infection-and-the-molecular-characteristics-of-mrsa-bacteraemia-over-a-two-year-period-in-a-tertiary-teaching-hospital-in-malaysia
#19
Pik San Sit, Cindy Shuan Ju Teh, Nuryana Idris, I-Ching Sam, Sharifah Faridah Syed Omar, Helmi Sulaiman, Kwai Lin Thong, Adeeba Kamarulzaman, Sasheela Ponnampalavanar
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is an established pathogen that causes hospital- and community-acquired infections worldwide. The prevalence rate of MRSA infections were reported to be the highest in Asia. As there is limited epidemiological study being done in Malaysia, this study aimed to determine the prevalence of MRSA infection and the molecular characteristics of MRSA bacteraemia. METHODS: Two hundred and nine MRSA strains from year 2011 to 2012 were collected from a tertiary teaching hospital in Malaysia...
April 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28406770/frequency-and-antibiogram-of-isolates-from-surgical-units-of-a-tertiary-care-hospital-in-quetta
#20
Maria Mushtaq Gill, Muhammad Junaid Khan, Muhammad Asif Nawaz, Sarfraz Khan Janjua
OBJECTIVE: To determine the frequency and antibiogram of the isolates from infected patients in surgical units of a tertiary care hospital. STUDY DESIGN: Cross-sectional, descriptive study. PLACE AND DURATION OF STUDY: Department of Microbiology, Combined Military Hospital, Quetta, from March to October 2015. METHODOLOGY: Clinical samples from the surgical units received in Department of Microbiology for culture and sensitivity were analyzed by Gram stain, culture and biochemical tests for identification of the isolates; and the antibiotic susceptibility was determined by modified Kirby Bauer disc diffusion method...
March 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
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