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https://www.readbyqxmd.com/read/28223006/complete-genome-sequence-of-the-drought-resistance-promoting-endophyte-klebsiella-sp-ltgpaf-6f
#1
Lei Zhang, Jun Zhong, Hao Liu, Kaiyun Xin, Chaoqiong Chen, Qiqi Li, Yahong Wei, Yao Wang, Fei Chen, Xihui Shen
Bacterial endophytes with capacity to promote plant growth and improve plant tolerance against biotic and abiotic stresses have importance in agricultural practice and phytoremediation. A plant growth-promoting endophyte named Klebsiella sp. LTGPAF-6F, which was isolated from the roots of the desert plant Alhagi sparsifolia in north-west China, exhibits the ability to enhance the growth of wheat under drought stress. The complete genome sequence of this strain consists of one circular chromosome and two circular plasmids...
February 13, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28214755/identification-of-a-new-tert-leucinate-class-synthetic-cannabinoid-in-powder-and-spice-like-herbal-incenses-methyl-2-1-5-fluoropentyl-indole-3-carbonyl-amino-3-3-dimethyl-butanoate-5f-mdmb-pica
#2
Martijn D P Risseeuw, Peter Blanckaert, Vera Coopman, Stijn Van Quekelberghe, Serge Van Calenbergh, Jan Cordonnier
A brown powder and different product packages of "spice-like" herbal incenses were analyzed using a systematic identification approach based on liquid chromatography with diode array detector (HPLC-PDA) and gas chromatography mass spectrometry (GC-MS) with computer based library search against spectral libraries. However, the most predominant compound in the methanolic sample solutions could not be identified. In order to elucidate the chemical structure, a more extensive analysis of the material was initiated using liquid chromatography mass spectrometry (LC-MS), electrospray high resolution mass spectrometry (ESI-HRMS) and (1)H, (13)C and (19)F nuclear magnetic resonance spectroscopy (NMR), which allowed the identification and characterization of the major compound as methyl 2-[[1-(5-fluoropentyl)indole-3-carbonyl]amino]-3,3-dimethyl-butanoate (5F-MDMB-PICA)...
February 4, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28214444/the-boosted-biomass-and-lipid-accumulation-in-chlorella-vulgaris-by-supplementation-of-synthetic-phytohormone-analogs
#3
Tingting Liu, Fei Liu, Chao Wang, Zhenyao Wang, Yuqin Li
This study attempted at maximizing biomass and lipid accumulation in Chlorella vulgaris by supplementation of natural abscisic acid (ABA) or synthetic 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) hormone analogs. Amongst three tested additives, NAA-treatment performed remarkable promoting effect on cell growth and lipid biosynthesis. The favorable lipid productivity (418.6mg/L/d) of NAA-treated cells showed 1.48 and 2.24 times more than that of 2,4-D and ABA. NAA-treatment also positively modified the proportions of saturated (C16:0 and C18:0) and monounsaturated fatty acids (C18:1) which were prone to high-quality biofuels-making...
February 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28212039/in-vitro-stepwise-reconstitution-of-amino-acid-derived-vinyl-isocyanide-biosynthesis-detection-of-an-elusive-intermediate
#4
Wei-Chen Chang, Dev Sanyal, Jhih-Liang Huang, Kuljira Ittiamornkul, Qin Zhu, Xinyu Liu
In vitro reconstitution of a newly discovered isonitrile synthase (AmbI1 and AmbI2) and the detection of an elusive intermediate (S)-3-(1H-indol-3-yl)-2-isocyanopropanoic acid 1 in indolyl vinyl isocyanide biogenesis are reported. The characterization of iron/2-oxoglutarate (Fe/2OG) dependent desaturases IsnB and AmbI3 sheds light on the possible mechanism underlying stereoselective alkene installation to complete the biosynthesis of (E)- and (Z)-3-(2-isocyanovinyl)-1H-indole 2 and 5. Establishment of a tractable isonitrile synthase system (AmbI1 and AmbI2) paves the way to elucidate the enigmatic enzyme mechanism for isocyanide formation...
February 17, 2017: Organic Letters
https://www.readbyqxmd.com/read/28211339/phytoextraction-of-contaminated-urban-soils-by-panicum-virgatum-l-enhanced-with-application-of-a-plant-growth-regulator-bap-and-citric-acid
#5
Matthew Aderholt, Dale L Vogelien, Marina Koether, Sigurdur Greipsson
Lead (Pb) contamination in soil represents a threat to human health. Phytoextraction has gained attention as a potential alternative to traditional remediation methods because of lower cost and minimal soil disruption. The North American native switchgrass (Panicum virgatum L.) was targeted due to its ability to produce high biomass and grow across a variety of ecozones. In this study switchgrass was chemically enhanced with applications of the soil-fungicide benomyl, chelates (EDTA and citric acid), and PGR to optimize phytoextraction of Pb and zinc (Zn) from contaminated urban soils in Atlanta, GA...
February 4, 2017: Chemosphere
https://www.readbyqxmd.com/read/28209181/de-novo-sequencing-and-comparative-transcriptome-analysis-of-adventitious-root-development-induced-by-exogenous-indole-3-butyric-acid-in-cuttings-of-tetraploid-black-locust
#6
Jine Quan, Seng Meng, Erhui Guo, Sheng Zhang, Zhong Zhao, Xitian Yang
BACKGROUND: Indole-3-butyric acid (IBA) is applied to the cuttings of various plant species to induce formation of adventitious roots (ARs) in commercial settings. Tetraploid black locust is an attractive ornamental tree that is drought resistant, sand tolerant, can prevent sand erosion and has various commercial uses. To further elucidate the mechanisms of AR formation, we used Illumina sequencing to analyze transcriptome dynamics and differential gene expression at four developmental stages in control (CK) and IBA-treated groups...
February 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28197647/the-overproduction-of-indole-3-acetic-acid-iaa-in-endophytes-upregulates-nitrogen-fixation-in-both-bacterial-cultures-and-inoculated-rice-plants
#7
Roberto Defez, Anna Andreozzi, Carmen Bianco
Endophytic bacteria from roots and leaves of rice plants were isolated and identified in order to select the diazotrophs and improve their nitrogen-fixing abilities. The nitrogen-fixing endophytes were identified by PCR amplification of the nifH gene fragment. For this purpose, two isolates, Enterobacter cloacae RCA25 and Klebsiella variicola RCA26, and two model bacteria (Herbaspirillum seropedicae z67 and Sinorhizobium fredii NGR234) were transformed to increase the biosynthesis of the main plant auxin indole-3-acetic acid (IAA)...
February 14, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28197562/n-heterocyclic-carbene-nhc-catalysed-atom-economical-construction-of-2-3-disubstituted-indoles
#8
Battu Harish, Manyam Subbireddy, Surisetti Suresh
A novel organocatalytic approach, harnessing the unique reactivities of N-heterocyclic carbenes (NHCs), has been revealed for the construction of indoles. The NHC-catalysed atom economical synthesis of a wide range of 2-substituted indole-3-acetic acid derivatives is displayed. Strategic application of the developed method was demonstrated for a short synthesis of a cyclin-dependent kinase (CDK) inhibitor: paullone.
February 15, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28187139/complete-genome-analysis-of-serratia-marcescens-rsc-14-a-plant-growth-promoting-bacterium-that-alleviates-cadmium-stress-in-host-plants
#9
Abdur Rahim Khan, Gun-Seok Park, Sajjad Asaf, Sung-Jun Hong, Byung Kwon Jung, Jae-Ho Shin
Serratia marcescens RSC-14 is a Gram-negative bacterium that was previously isolated from the surface-sterilized roots of the Cd-hyperaccumulator Solanum nigrum. The strain stimulates plant growth and alleviates Cd stress in host plants. To investigate the genetic basis for these traits, the complete genome of RSC-14 was obtained by single-molecule real-time sequencing. The genome of S. marcescens RSC-14 comprised a 5.12-Mbp-long circular chromosome containing 4,593 predicted protein-coding genes, 22 rRNA genes, 88 tRNA genes, and 41 pseudogenes...
2017: PloS One
https://www.readbyqxmd.com/read/28180138/er-microsome-preparation-and-subsequent-iaa-quantification-in-maize-coleoptile-and-primary-root-tissue
#10
Verena Kriechbaumer
Auxin is a major growth hormone in plants and the first plant hormone to be discovered and studied (Darwin and Darwin, 1880). The auxin molecule in plants was first identified as indole-3-acetic acid (IAA) by Kögl et al. (1934). Active research over nearly a decade has shed light on many of the molecular mechanisms of its action but the complexity and redundancy of the auxin biosynthetic network raises questions about control of this system. We have shown that some enzymes involved in the YUCCA-route of auxin biosynthesis are not cytosolic but localised to the endoplasmic reticulum (ER) in both Arabidopsis thaliana (YUCCA4...
May 5, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28177844/genome-wide-analysis-and-expression-characteristics-of-small-auxin-up-rna-saur-genes-in-moso-bamboo-phyllostachys-edulis
#11
Qingsong Bai, Dan Hou, Long Li, Zhanchao Cheng, Wei Ge, Jun Liu, Xueping Li, Shaohua Mu, Jian Gao
Moso bamboo (Phyllostachys edulis) is well-known for its rapid shoot growth. Auxin exerts pleiotropic effects on plant growth. The small auxin-up RNA (SAUR) genes are early auxin-responsive genes involved in plant growth. In total, 38 SAUR genes were identified in P. edulis (PheSAUR). A comprehensive overview of the PheSAUR gene family is presented, including the gene structures, phylogeny and subcellular location predictions. A transcriptome analysis indicated that 37 (except PheSAUR18) of the PheSAUR genes were expressed during shoot growth process and the PheSAUR genes were differentially expressed...
November 15, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177802/plant-growth-promotion-properties-of-bacterial-strains-isolated-from-the-rhizosphere-of-the-jerusalem-artichoke-helianthus-tuberosus-l-adapted-to-saline-alkaline-soils-and-their-effect-on-wheat-growth
#12
Xiaolin Liu, Xiangyue Li, Yan Li, Runzhi Li, Zhihong Xie
The Jerusalem artichoke (JA; Helianthus tuberosus), known to be tolerant to saline-alkaline soil conditions, has been cultivated for many years in the Yellow River delta, Shandong Province coastal zone, in China. The aim of our study was to isolate nitrogen-fixing bacteria colonizing the rhizosphere of JA and to characterize other plant growth promotion properties. The ultimate goal was to identify isolates that could be used as inoculants benefiting an economic crop, in particular for improving wheat growth production in the Yellow River delta...
November 14, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28174680/structural-evidence-for-arabidopsis-glutathione-transferase-atgstf2-functioning-as-a-transporter-of-small-organic-ligands
#13
Laziana Ahmad, Elizabeth L Rylott, Neil C Bruce, Robert Edwards, Gideon Grogan
Glutathione transferases (GSTs) are involved in many processes in plant biochemistry, with their best characterised role being the detoxification of xenobiotics through their conjugation with glutathione. GSTs have also been implicated in noncatalytic roles, including the binding and transport of small heterocyclic ligands such as indole hormones, phytoalexins and flavonoids. Although evidence for ligand binding and transport has been obtained using gene deletions and ligand binding studies on purified GSTs, there has been no structural evidence for the binding of relevant ligands in noncatalytic sites...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174581/arabidopsis-nitrilase-1-contributes-to-the-regulation-of-root-growth-and-development-through-modulation-of-auxin-biosynthesis-in-seedlings
#14
Thomas Lehmann, Tim Janowitz, Beatriz Sánchez-Parra, Marta-Marina Pérez Alonso, Inga Trompetter, Markus Piotrowski, Stephan Pollmann
Nitrilases consist of a group of enzymes that catalyze the hydrolysis of organic cyanides. They are found ubiquitously distributed in the plant kingdom. Plant nitrilases are mainly involved in the detoxification of ß-cyanoalanine, a side-product of ethylene biosynthesis. In the model plant Arabidopsis thaliana a second group of Brassicaceae-specific nitrilases (NIT1-3) has been found. This so-called NIT1-subfamily has been associated with the conversion of indole-3-acetonitrile (IAN) into the major plant growth hormone, indole-3-acetic acid (IAA)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28163278/bacterial-compatibility-in-combined-inoculations-enhances-the-growth-of-potato-seedlings
#15
Christine D Santiago, Shogo Yagi, Motoaki Ijima, Tomoya Nashimoto, Maki Sawada, Seishi Ikeda, Kenji Asano, Yoshitake Orikasa, Takuji Ohwada
The compatibility of strains is crucial for formulating bioinoculants that promote plant growth. We herein assessed the compatibility of four potential bioinoculants isolated from potato roots and tubers (Sphingomonas sp. T168, Streptomyces sp. R170, Streptomyces sp. R181, and Methylibium sp. R182) that were co-inoculated in order to improve plant growth. We screened these strains using biochemical tests, and the results obtained showed that R170 had the highest potential as a bioinoculant, as indicated by its significant ability to produce plant growth-promoting substances, its higher tolerance against NaCl (2%) and AlCl3 (0...
February 4, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28158805/integration-of-auxin-indole-3-acetic-acid-17-and-rga-like3-confers-salt-stress-resistance-through-stabilization-by-nitric-oxide-in-arabidopsis
#16
Haitao Shi, Wen Liu, Yunxie Wei, Tiantian Ye
No abstract text is available yet for this article.
January 31, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28138057/biochemical-and-chemical-biology-study-of-rice-ostar1-revealed-that-tryptophan-aminotransferase-is-involved-in-auxin-biosynthesis-identification-of-a-potent-ostar1-inhibitor-pyruvamine2031
#17
Yusuke Kakei, Ayako Nakamura, Mitsuhiro Yamamoto, Yosuke Ishida, Chiaki Yamazaki, Akiko Sato, Megumi Narukawa-Nara, Kazuo Soeno, Yukihisa Shimada
Indole-3-acetic acid (IAA), a major form of auxin, is biosynthesized from L-tryptophan via the indole-3-pyruvic acid (IPyA) pathway in Arabidopsis Tryptophan aminotransferases (TAA1/TARs) catalyze the first step from L-tryptophan to IPyA. In rice, the importance of TAA/TARs or YUC homologs in auxin biosynthesis has been suggested, but the enzymatic activities and involvement of the intermediate IPyA in auxin biosynthesis remain elusive. In this study, we obtained biochemical evidence that the rice tryptophan aminotransferase OsTAR1 converts L-tryptophan to IPyA, and has a Km of 82...
January 30, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28137215/targeting-cancer-stem-cells-for-chemoprevention-of-pancreatic-cancer
#18
Dharmalingam Subramaniam, Gaurav Kaushik, Prasad Dandawate, Shrikant Anant
Pancreatic ductal adenocarcinoma is one of the deadliest cancers worldwide and the fourth leading cause of cancer-related deaths in United States. Regardless of the advances in molecular pathogenesis and consequential efforts to suppress the disease, this cancer remains a major health problem in United States. By 2030, the projection is that pancreatic cancer will be climb up to be the second leading cause of cancer-related deaths in the United States. Pancreatic cancer is a rapidly invasive and highly metastatic cancer, and does not respond to standard therapies...
January 26, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28135698/graphene-oxide-sio2-nanocomposite-as-the-adsorbent-for-extraction-and-preconcentration-of-plant-hormones-for-hplc-analysis
#19
Xiaona Zhang, Jiahua Niu, Xiaoting Zhang, Rui Xiao, Minghua Lu, Zongwei Cai
In this research, a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method based on graphene oxide@SiO2(SiO2@GO) nanocomposite as adsorbent of dispersive solid-phase extraction (dSPE) combined with high performance liquid chromatography (HPLC) for the analysis of four plant hormones in different plants was established. The as-prepared SiO2@GO was characterized by scanning electron microscopy, transmission electron microscopy and infrared spectroscopy. The experimental conditions for dSPE, including the ratio of material to liquid, pH of sample, adsorption and desorption time, desorption temperature as well as desorption solution, were investigated...
January 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28134574/ameliorates-of-salt-stress-on-bermudagrass-by-the-fungus-aspergillus-aculeatus
#20
Yan Xie, Shijuan Han, Xiaoning Li, Erick Amombo, Jinmin Fu
There is considerable evidence that plants' abiotic-stress tolerance can be evoked by the exploitation of globally abundant microbe. A.aculeatus, which was initially isolated from the rhizosphere of bermudagrass, has been shown to increase heavy metal tolerance in turfgrasses. Here, we report on the potential of A.aculeatus to induce tolerance to salt stress in bermudagrass. Physiological markers for salt stress, such as plant growth rate, lipid peroxidation, photosynthesis and ionic homeostasis were assessed...
January 30, 2017: Molecular Plant-microbe Interactions: MPMI
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