Amanda G Charlesworth, Uri Seroussi, Nicolas J Lehrbach, Mathias S Renaud, Adam E Sundby, Ruxandra I Molnar, Robert X Lao, Alexandra R Willis, Jenna R Woock, Matthew J Aber, Annette J Diao, Aaron W Reinke, Gary Ruvkun, Julie M Claycomb
The Caenorhabditis elegans genome encodes nineteen functional Argonaute proteins that use 22G-RNAs, 26G-RNAs, miRNAs or piRNAs to regulate target transcripts. Only one Argonaute is essential under normal laboratory conditions: CSR-1. While CSR-1 has been studied widely, nearly all studies have overlooked the fact that the csr-1 locus encodes two isoforms. These isoforms differ by an additional 163 amino acids present in the N-terminus of CSR-1a. Using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG into the long (CSR-1a) and short (CSR-1b) isoforms, we found that CSR-1a is expressed during spermatogenesis and in several somatic tissues, including the intestine...
September 7, 2021: Nucleic Acids Research