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Amy Wagers

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https://www.readbyqxmd.com/read/27906115/established-cell-surface-markers-efficiently-isolate-highly-overlapping-populations-of-skeletal-muscle-satellite-cells-by-fluorescence-activated-cell-sorting
#1
Claire C Maesner, Albert E Almada, Amy J Wagers
BACKGROUND: Fluorescent-activated cell sorting (FACS) has enabled the direct isolation of highly enriched skeletal muscle stem cell, or satellite cell, populations from postnatal tissue. Several distinct surface marker panels containing different positively selecting surface antigens have been used to distinguish muscle satellite cells from other non-myogenic cell types. Because functional and transcriptional heterogeneity is known to exist within the satellite cell population, a direct comparison of results obtained in different laboratories has been complicated by a lack of clarity as to whether commonly utilized surface marker combinations select for distinct or overlapping subsets of the satellite cell pool...
November 8, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27881824/two-tissue-resident-progenitor-lineages-drive-distinct-phenotypes-of-heterotopic-ossification
#2
Devaveena Dey, Jana Bagarova, Sarah J Hatsell, Kelli A Armstrong, Lily Huang, Joerg Ermann, Ashley J Vonner, Yue Shen, Agustin H Mohedas, Arthur Lee, Elisabeth M W Eekhoff, Annelies van Schie, Marie B Demay, Charles Keller, Amy J Wagers, Aris N Economides, Paul B Yu
Fibrodysplasia ossificans progressiva (FOP), a congenital heterotopic ossification (HO) syndrome caused by gain-of-function mutations of bone morphogenetic protein (BMP) type I receptor ACVR1, manifests with progressive ossification of skeletal muscles, tendons, ligaments, and joints. In this disease, HO can occur in discrete flares, often triggered by injury or inflammation, or may progress incrementally without identified triggers. Mice harboring an Acvr1(R206H) knock-in allele recapitulate the phenotypic spectrum of FOP, including injury-responsive intramuscular HO and spontaneous articular, tendon, and ligament ossification...
November 23, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27852976/age-associated-nf-%C3%AE%C2%BAb-signaling-in-myofibers-alters-the-satellite-cell-niche-and-re-strains-muscle-stem-cell-function
#3
Juhyun Oh, Indranil Sinha, Kah Yong Tan, Bernard Rosner, Jonathan M Dreyfuss, Ornela Gjata, Peter Tran, Steven E Shoelson, Amy J Wagers
Skeletal muscle is a highly regenerative tissue, but muscle repair potential is increasingly compromised with advancing age. In this study, we demonstrate that increased NF-κB activity in aged muscle fibers contributes to diminished myogenic potential of their associated satellite cells. We further examine the impact of genetic modulation of NF-κB signaling in muscle satellite cells or myofibers on recovery after damage. These studies reveal that NF-κB activity in differentiated myofibers is sufficient to drive dysfunction of muscle regenerative cells via cell-non-autonomous mechanisms...
November 14, 2016: Aging
https://www.readbyqxmd.com/read/27826411/established-cell-surface-markers-efficiently-isolate-highly-overlapping-populations-of-skeletal-muscle-satellite-cells-by-fluorescence-activated-cell-sorting
#4
Claire C Maesner, Albert E Almada, Amy J Wagers
BACKGROUND: Fluorescent-activated cell sorting (FACS) has enabled the direct isolation of highly enriched skeletal muscle stem cell, or satellite cell, populations from postnatal tissue. Several distinct surface marker panels containing different positively selecting surface antigens have been used to distinguish muscle satellite cells from other non-myogenic cell types. Because functional and transcriptional heterogeneity is known to exist within the satellite cell population, a direct comparison of results obtained in different laboratories has been complicated by a lack of clarity as to whether commonly utilized surface marker combinations select for distinct or overlapping subsets of the satellite cell pool...
2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27620167/initiation-influence-and-impact-adolescents-and-parents-discuss-the-marketing-of-gambling-products-during-australian-sporting-matches
#5
Hannah Pitt, Samantha L Thomas, Amy Bestman
BACKGROUND: Harmful gambling is a significant public health issue. Alongside the rapid diversification of gambling products, are rapid increases in the marketing for specific types of gambling products, such as online wagering. While concern has been raised about the impact of gambling promotions during sporting matches on the gambling beliefs and behaviours of adolescents, very little research has explored adolescents' and parents' attitudes towards the marketing of gambling products within sport...
2016: BMC Public Health
https://www.readbyqxmd.com/read/27595405/a-multifunctional-aav-crispr-cas9-and-its-host-response
#6
Wei Leong Chew, Mohammadsharif Tabebordbar, Jason K W Cheng, Prashant Mali, Elizabeth Y Wu, Alex H M Ng, Kexian Zhu, Amy J Wagers, George M Church
CRISPR-Cas9 delivery by adeno-associated virus (AAV) holds promise for gene therapy but faces critical barriers on account of its potential immunogenicity and limited payload capacity. Here, we demonstrate genome engineering in postnatal mice using AAV-split-Cas9, a multifunctional platform customizable for genome editing, transcriptional regulation, and other previously impracticable applications of AAV-CRISPR-Cas9. We identify crucial parameters that impact efficacy and clinical translation of our platform, including viral biodistribution, editing efficiencies in various organs, antigenicity, immunological reactions, and physiological outcomes...
October 2016: Nature Methods
https://www.readbyqxmd.com/read/27526034/the-vitamin-d-receptor-regulates-tissue-resident-macrophage-response-to-injury
#7
Lige Song, Garyfallia Papaioannou, Hengguang Zhao, Hilary F Luderer, Christine Miller, Claudia Dall'Osso, Rosalynn M Nazarian, Amy J Wagers, Marie B Demay
Ligand-dependent actions of the vitamin D receptor (VDR) play a pleiotropic role in the regulation of innate and adaptive immunity. The liganded VDR is required for recruitment of macrophages during the inflammatory phase of cutaneous wound healing. Although the number of macrophages in the granulation tissue 2 days after wounding is markedly reduced in VDR knockout (KO) compared with wild-type mice, VDR ablation does not alter macrophage polarization. Parabiosis studies demonstrate that circulatory chimerism with wild-type mice is unable to rescue the macrophage defect in the wounds of VDR KO mice and reveal that wound macrophages are of local origin, regardless of VDR status...
October 2016: Endocrinology
https://www.readbyqxmd.com/read/27524502/-it-s-just-everywhere-children-and-parents-discuss-the-marketing-of-sports-wagering-in-australia
#8
Hannah Pitt, Samantha L Thomas, Amy Bestman, Melissa Stoneham, Mike Daube
OBJECTIVE: To investigate how children and adults recall the content and promotional channels for sports wagering marketing. METHODS: A mixed methods study of 152 parent/child (8-16 years) dyads was conducted at AFL (Australian Football League), NRL (National Rugby League), and soccer sporting sites in New South Wales and Victoria, Australia. Questions related to the frequency of viewing AFL and NRL matches, sports wagering promotions and perceptions of the normalisation of wagering in sport...
October 2016: Australian and New Zealand Journal of Public Health
https://www.readbyqxmd.com/read/27401346/developmental-regulation-of-myeloerythroid-progenitor-function-by-the-lin28b-let-7-hmga2-axis
#9
R Grant Rowe, Leo D Wang, Silvia Coma, Areum Han, Ronald Mathieu, Daniel S Pearson, Samantha Ross, Patricia Sousa, Phi T Nguyen, Antony Rodriguez, Amy J Wagers, George Q Daley
For appropriate development, tissue and organ system morphogenesis and maturation must occur in synchrony with the overall developmental requirements of the host. Mistiming of such developmental events often results in disease. The hematopoietic system matures from the fetal state, characterized by robust erythrocytic output that supports prenatal growth in the hypoxic intrauterine environment, to the postnatal state wherein granulocytes predominate to provide innate immunity. Regulation of the developmental timing of these myeloerythroid states is not well understood...
July 25, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27396512/aging-and-rejuvenation-insights-from-rusty-gage-leonard-guarente-and-amy-wagers
#10
Fred H Gage, Leonard P Guarente, Amy J Wagers
No abstract text is available yet for this article.
August 2016: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/27365422/phosphoproteomic-profiling-of-mouse-primary-hspcs-reveals-new-regulators-of-hspc-mobilization
#11
Leo D Wang, Scott B Ficarro, John N Hutchinson, Roland Csepanyi-Komi, Phi T Nguyen, Eva Wisniewski, Jessica Sullivan, Oliver Hofmann, Erzsebet Ligeti, Jarrod A Marto, Amy J Wagers
Protein phosphorylation is a central mechanism of signal transduction that both positively and negatively regulates protein function. Large-scale studies of the dynamic phosphorylation states of cell signaling systems have been applied extensively in cell lines and whole tissues to reveal critical regulatory networks, and candidate-based evaluations of phosphorylation in rare cell populations have also been informative. However, application of comprehensive profiling technologies to adult stem cell and progenitor populations has been challenging, due in large part to the scarcity of such cells in adult tissues...
September 15, 2016: Blood
https://www.readbyqxmd.com/read/27217486/overexpressing-irs1-in-endothelial-cells-enhances-angioblast-differentiation-and-wound-healing-in-diabetes-and-insulin-resistance
#12
Sayaka Katagiri, Kyoungmin Park, Yasutaka Maeda, Tata Nageswara Rao, Mogher Khamaisi, Qian Li, Hisashi Yokomizo, Akira Mima, Luca Lancerotto, Amy Wagers, Dennis P Orgill, George L King
The effect of enhancing insulin's actions in endothelial cells (ECs) to improve angiogenesis and wound healing was studied in obesity and diabetes. Insulin receptor substrate 1 (IRS1) was overexpressed in ECs using the VE-cadherin promoter to create ECIRS1 TG mice, which elevated pAkt activation and expressions of vascular endothelial growth factor (VEGF), Flk1, and VE-cadherin in ECs and granulation tissues (GTs) of full-thickness wounds. Open wound and epithelialization rates and angiogenesis significantly improved in normal mice and high fat (HF) diet-induced diabetic mice with hyperinsulinemia in ECIRS1 TG versus wild type (WT), but not in insulin-deficient diabetic mice...
September 2016: Diabetes
https://www.readbyqxmd.com/read/27058668/egln1-inhibition-and-rerouting-of-%C3%AE-ketoglutarate-suffice-for-remote-ischemic-protection
#13
Benjamin A Olenchock, Javid Moslehi, Alan H Baik, Shawn M Davidson, Jeremy Williams, William J Gibson, Abhishek A Chakraborty, Kerry A Pierce, Christine M Miller, Eric A Hanse, Ameeta Kelekar, Lucas B Sullivan, Amy J Wagers, Clary B Clish, Matthew G Vander Heiden, William G Kaelin
No abstract text is available yet for this article.
April 7, 2016: Cell
https://www.readbyqxmd.com/read/27034275/biochemistry-and-biology-of-gdf11-and-myostatin-similarities-differences-and-questions-for-future-investigation
#14
REVIEW
Ryan G Walker, Tommaso Poggioli, Lida Katsimpardi, Sean M Buchanan, Juhyun Oh, Sam Wattrus, Bettina Heidecker, Yick W Fong, Lee L Rubin, Peter Ganz, Thomas B Thompson, Amy J Wagers, Richard T Lee
Growth differentiation factor 11 (GDF11) and myostatin (or GDF8) are closely related members of the transforming growth factor β superfamily and are often perceived to serve similar or overlapping roles. Yet, despite commonalities in protein sequence, receptor utilization and signaling, accumulating evidence suggests that these 2 ligands can have distinct functions in many situations. GDF11 is essential for mammalian development and has been suggested to regulate aging of multiple tissues, whereas myostatin is a well-described negative regulator of postnatal skeletal and cardiac muscle mass and modulates metabolic processes...
April 1, 2016: Circulation Research
https://www.readbyqxmd.com/read/26956195/molecular-circuitry-of-stem-cell-fate-in-skeletal-muscle-regeneration-ageing-and-disease
#15
Albert E Almada, Amy J Wagers
Satellite cells are adult myogenic stem cells that repair damaged muscle. The enduring capacity for muscle regeneration requires efficient satellite cell expansion after injury, their differentiation to produce myoblasts that can reconstitute damaged fibres and their self-renewal to replenish the muscle stem cell pool for subsequent rounds of injury and repair. Emerging studies indicate that misregulation of satellite cell fate and function can contribute to age-associated muscle dysfunction and influence the severity of muscle diseases, including Duchenne muscular dystrophy (DMD)...
May 2016: Nature Reviews. Molecular Cell Biology
https://www.readbyqxmd.com/read/26919427/egln1-inhibition-and-rerouting-of-%C3%AE-ketoglutarate-suffice-for-remote-ischemic-protection
#16
Benjamin A Olenchock, Javid Moslehi, Alan H Baik, Shawn M Davidson, Jeremy Williams, William J Gibson, Abhishek A Chakraborty, Kerry A Pierce, Christine M Miller, Eric A Hanse, Ameeta Kelekar, Lucas B Sullivan, Amy J Wagers, Clary B Clish, Matthew G Vander Heiden, William G Kaelin
Ischemic preconditioning is the phenomenon whereby brief periods of sublethal ischemia protect against a subsequent, more prolonged, ischemic insult. In remote ischemic preconditioning (RIPC), ischemia to one organ protects others organs at a distance. We created mouse models to ask if inhibition of the alpha-ketoglutarate (αKG)-dependent dioxygenase Egln1, which senses oxygen and regulates the hypoxia-inducible factor (HIF) transcription factor, could suffice to mediate local and remote ischemic preconditioning...
February 25, 2016: Cell
https://www.readbyqxmd.com/read/26872699/poor-repair-of-skeletal-muscle-in-aging-mice-reflects-a-defect-in-local-interleukin-33-dependent-accumulation-of-regulatory-t-cells
#17
Wilson Kuswanto, Dalia Burzyn, Marisella Panduro, Kathy K Wang, Young Charles Jang, Amy J Wagers, Christophe Benoist, Diane Mathis
Normal repair of skeletal muscle requires local expansion of a special population of Foxp3(+)CD4(+) regulatory T (Treg) cells. Such cells failed to accumulate in acutely injured muscle of old mice, known to undergo ineffectual repair. This defect reflected reduced recruitment of Treg cells to injured muscle, as well as less proliferation and retention therein. Interleukin-33 (IL-33) regulated muscle Treg cell homeostasis in young mice, and its administration to old mice ameliorated their deficits in Treg cell accumulation and muscle regeneration...
February 16, 2016: Immunity
https://www.readbyqxmd.com/read/26808499/pkc%C3%AE-inhibition-normalizes-the-wound-healing-capacity-of-diabetic-human-fibroblasts
#18
Mogher Khamaisi, Sayaka Katagiri, Hillary Keenan, Kyoungmin Park, Yasutaka Maeda, Qian Li, Weier Qi, Thomas Thomou, Danielle Eschuk, Ana Tellechea, Aris Veves, Chenyu Huang, Dennis Paul Orgill, Amy Wagers, George L King
Abnormal fibroblast function underlies poor wound healing in patients with diabetes; however, the mechanisms that impair wound healing are poorly defined. Here, we evaluated fibroblasts from individuals who had type 1 diabetes (T1D) for 50 years or more (Medalists, n = 26) and from age-matched controls (n = 7). Compared with those from controls, Medalist fibroblasts demonstrated a reduced migration response to insulin, lower VEGF expression, and less phosphorylated AKT (p-AKT), but not p-ERK, activation. Medalist fibroblasts were also functionally less effective at wound closure in nude mice...
March 1, 2016: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/26721686/in-vivo-gene-editing-in-dystrophic-mouse-muscle-and-muscle-stem-cells
#19
Mohammadsharif Tabebordbar, Kexian Zhu, Jason K W Cheng, Wei Leong Chew, Jeffrey J Widrick, Winston X Yan, Claire Maesner, Elizabeth Y Wu, Ru Xiao, F Ann Ran, Le Cong, Feng Zhang, Luk H Vandenberghe, George M Church, Amy J Wagers
Frame-disrupting mutations in the DMD gene, encoding dystrophin, compromise myofiber integrity and drive muscle deterioration in Duchenne muscular dystrophy (DMD). Removing one or more exons from the mutated transcript can produce an in-frame mRNA and a truncated, but still functional, protein. In this study, we developed and tested a direct gene-editing approach to induce exon deletion and recover dystrophin expression in the mdx mouse model of DMD. Delivery by adeno-associated virus (AAV) of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 endonucleases coupled with paired guide RNAs flanking the mutated Dmd exon23 resulted in excision of intervening DNA and restored the Dmd reading frame in myofibers, cardiomyocytes, and muscle stem cells after local or systemic delivery...
January 22, 2016: Science
https://www.readbyqxmd.com/read/26499495/functional-genomic-screening-reveals-asparagine-dependence-as-a-metabolic-vulnerability-in-sarcoma
#20
Simone Hettmer, Anna C Schinzel, Daria Tchessalova, Michaela Schneider, Christina L Parker, Roderick T Bronson, Nigel Gj Richards, William C Hahn, Amy J Wagers
Current therapies for sarcomas are often inadequate. This study sought to identify actionable gene targets by selective targeting of the molecular networks that support sarcoma cell proliferation. Silencing of asparagine synthetase (ASNS), an amidotransferase that converts aspartate into asparagine, produced the strongest inhibitory effect on sarcoma growth in a functional genomic screen of mouse sarcomas generated by oncogenic Kras and disruption of Cdkn2a. ASNS silencing in mouse and human sarcoma cell lines reduced the percentage of S phase cells and impeded new polypeptide synthesis...
2015: ELife
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