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https://www.readbyqxmd.com/read/27855103/hyaluronic-acid-binding-sperm-selection-for-assisted-reproduction-treatment-habselect-study-protocol-for-a-multicentre-randomised-controlled-trial
#1
K D Witt, L Beresford, S Bhattacharya, K Brian, A Coomarasamy, R Hooper, J Kirkman-Brown, Y Khalaf, S E Lewis, A Pacey, S Pavitt, R West, D Miller
INTRODUCTION: The selection of a sperm with good genomic integrity is an important consideration for improving intracytoplasmic sperm injection (ICSI) outcome. Current convention selects sperm by vigour and morphology, but preliminary evidence suggests selection based on hyaluronic acid binding may be beneficial. The aim of the Hyaluronic Acid Binding Sperm Selection (HABSelect) trial is to determine the efficacy of hyaluronic acid (HA)-selection of sperm versus conventionally selected sperm prior to ICSI on live birth rate (LBR)...
October 7, 2016: BMJ Open
https://www.readbyqxmd.com/read/27231097/intervention-improves-assisted-conception-intracytoplasmic-sperm-injection-outcomes-for-patients-with-high-levels-of-sperm-dna-fragmentation-a-retrospective-analysis
#2
C K Bradley, S J McArthur, A J Gee, K A Weiss, U Schmidt, L Toogood
Sperm DNA fragmentation (SDF) is used in assisted reproductive technology (ART) programs as an indicator for sperm quality, although there is still a lack of consensus as to its clinical utility. In this retrospective study, we examined intracytoplasmic sperm injection (ICSI) outcomes of 1924 infertile patients who underwent SDF analysis using the sperm chromatin integrity test. ART patients were classified as having low [DNA fragmentation index (DFI) <29%] or high SDF (DFI ≥29%) and by whether or not an intervention [physiological intracytoplasmic sperm injection (PICSI), intracytoplasmic morphologically selected sperm injection (IMSI), testicular sperm extraction (TESE)/testicular sperm aspiration (TESA), frequent ejaculation] was performed...
September 2016: Andrology
https://www.readbyqxmd.com/read/27206090/annexin-v-macs-in-infertile-couples-as-method-for-separation-of-sperm-without-dna-fragmentation
#3
Jhon Troya, Ingrid Zorrilla
OBJECTIVE: To determine the effect of using MACS technology on clinical pregnancy, as a method for separation of damaged sperm in infertile patients. METHODS: 136 infertile men having normal semen parameters in accordance with WHO 2010 criterion, undergoing ICSI cycle were enrolled during the course of the study. The patients were prospectively randomized and enrolled after oocyte retrieval and were assigned to the ICSI group, PICSI group or MACS group. Embryo development and clinical pregnancy were assessed...
2015: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/25348679/advanced-sperm-selection-techniques-for-assisted-reproduction
#4
REVIEW
Simon McDowell, Ben Kroon, Emily Ford, Ysanne Hook, Demián Glujovsky, Anusch Yazdani
BACKGROUND: Assisted reproductive technologies (ART) such as in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) bring together gametes outside of the body to enhance the probability of fertilisation and pregnancy. Advanced sperm selection techniques are increasingly being employed in ART, most commonly in cycles utilising ICSI. Advanced sperm selection techniques are thought to improve the chance that structurally intact and mature sperm with high DNA integrity are selected for fertilisation...
2014: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/25119820/is-sperm-hyaluronic-acid-binding-ability-predictive-for-clinical-success-of-intracytoplasmic-sperm-injection-picsi-vs-icsi
#5
COMPARATIVE STUDY
Attila Mokánszki, Emese Varga Tóthné, Béla Bodnár, Zoltán Tándor, Zsuzsanna Molnár, Attila Jakab, Anikó Ujfalusi, Éva Oláh
Although intracytoplasmic sperm injection (ICSI) is now a widely-used technique, it is still of interest to improve our knowledge as to which is the best spermatozoon to be selected for ICSI. Infertile men have increased risks of producing aneuploid spermatozoa. Using hyaluronic acid (HA)-binding sperm selection may reduce the genetic risks such as chromosomal aberrations of offspring. In the present study we examined the clinical success of ICSI with HA-selected sperm ('physiologic' ICSI, PICSI) compared to conventional ICSI, as well as the necessity to differentiate patients according to the initial HA-binding assay result (HBA score) and whether the sperm concentration or HBA score can provide additional information...
December 2014: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/24085466/a-prospective-randomized-study-to-evaluate-the-effect-of-hyaluronic-acid-sperm-selection-on-the-intracytoplasmic-sperm-injection-outcome-of-patients-with-unexplained-infertility-having-normal-semen-parameters
#6
RANDOMIZED CONTROLLED TRIAL
Gaurav Majumdar, Abha Majumdar
PURPOSE: Sperm quality plays an important role in determining embryo development and intracytoplasmic sperm injection (ICSI) outcome. Selection of competent sperm based on its ability to bind to hyaluronic acid (HA) has been suggested as one of the methods to assess sperm quality. The aim of the present study was to examine whether injection of HA bound sperm helps in improving outcome in patients undergoing ICSI with unexplained infertility having normal semen parameters. METHODS: Patients with unexplained infertility having normal semen parameters in accordance with WHO 2010 criterion, undergoing their first IVF-ICSI cycle were enrolled during the course of the study...
November 2013: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/22749221/comparison-of-two-ready-to-use-systems-designed-for-sperm-hyaluronic-acid-binding-selection-before-intracytoplasmic-sperm-injection-picsi-vs-sperm-slow-a-prospective-randomized-trial
#7
RANDOMIZED CONTROLLED TRIAL
Lodovico Parmegiani, Graciela Estela Cognigni, Silvia Bernardi, Enzo Troilo, Stefania Taraborrelli, Alessandra Arnone, Antonio Manuel Maccarini, Marco Filicori
OBJECTIVE: To compare, in a strict, randomized way, the efficiency of two ready-to-use systems for hyaluronic acid (HA)-intracytoplasmic sperm injection (ICSI): an HA culture dish (PICSI Sperm Selection Device) and a viscous medium containing HA (Sperm Slow). DESIGN: Prospective, randomized study. SETTING: Medical center. PATIENT(S): Fifty subjects per treatment group (100 total). INTERVENTION(S): One hundred ICSI treatments were randomly carried out with PICSI or with Sperm Slow for sperm selection...
September 2012: Fertility and Sterility
https://www.readbyqxmd.com/read/22702071/-new-methods-increasing-assisted-reproduction-results
#8
J Záková, P Ventruba, I Crha, E Lousová, K Sochorová, M Pohanka, M Huser
OBJECTIVE: The brief review of historical development and application of the assisted reproduction methods at our centre and the recent methods increasing the assisted reproduction results. The new mentioned methods are sperm selection before the intracytoplazmatic sperm injection (PICSI = preselected sperm intracytoplasmic injection) and continuous embryo development monitoring. DESIGN: Review article.
April 2012: Ceská Gynekologie
https://www.readbyqxmd.com/read/22138904/sperm-preparation-state-of-the-art-physiological-aspects-and-application-of-advanced-sperm-preparation-methods
#9
REVIEW
Ralf Henkel
For assisted reproduction technologies (ART), numerous techniques were developed to isolate spermatozoa capable of fertilizing oocytes. While early methodologies only focused on isolating viable, motile spermatozoa, with progress of ART, particularly intracytoplasmic sperm injection (ICSI), it became clear that these parameters are insufficient for the identification of the most suitable spermatozoon for fertilization. Conventional sperm preparation techniques, namely, swim-up, density gradient centrifugation and glass wool filtration, are not efficient enough to produce sperm populations free of DNA damage, because these techniques are not physiological and not modeled on the stringent sperm selection processes taking place in the female genital tract...
March 2012: Asian Journal of Andrology
https://www.readbyqxmd.com/read/18315179/bernas-ion-source-discharge-simulation
#10
I Roudskoy, T V Kulevoy, S V Petrenko, R P Kuibeda, D N Seleznev, V I Pershin, A Hershcovitch, B M Johnson, V I Gushenets, E M Oks, H P Poole
As the technology and applications continue to grow up, the development of plasma and ion sources with clearly specified characteristic is required. Therefore comprehensive numerical studies at the project stage are the key point for ion implantation source manufacturing (especially for low energy implantation). Recently the most commonly encountered numerical approach is the Monte Carlo particle-in-cell (MCPIC) method also known as particle-in-cell method with Monte Carlo collisions. In ITEP the 2D3V numerical code PICSIS-2D realizing MCPIC method was developed in the framework of the joint research program...
February 2008: Review of Scientific Instruments
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