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https://www.readbyqxmd.com/read/28335329/nanoparticle-based-delivery-of-anaplasma-marginale-membrane-proteins-virb9-1-and-virb10-produced-in-the-pichia-pastoris-expression-system
#1
Bing Zhang, Antonio S Cavallaro, Karishma T Mody, Jun Zhang, James R Deringer, Wendy C Brown, Timothy J Mahony, Chengzhong Yu, Neena Mitter
Bovine anaplasmosis or cattle-tick fever is a tick-borne haemolytic disease caused by the rickettsial haemoparasite Anaplasma marginale in tropical and subtropical areas of the world. While difficult to express, the proteins VirB9-1 and VirB10 are immunogenic components of the outer membrane type IV secretion system that have been identified as candidate antigens for vaccines targeting of A. marginale. Soluble VirB9-1 and VirB10 were successfully expressed using Pichia pastoris. When formulated with the self-adjuvanting silica vesicles, SV-100 (diameter: 50 nm, and pore entrance size: 6 nm), 200 µg of VirB9-1 and VirB10 were adsorbed per milligram of nanoparticle...
November 5, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28327271/novel-anti-staphylococcal-and-anti-biofilm-properties-of-two-anti-malarial-compounds-mmv665953-1-3-chloro-4-fluorophenyl-3-3-4-dichlorophenyl-urea-and-mmv665807-5-chloro-2-hydroxy-n-3-trifluoromethyl-phenyl-benzamide
#2
Marta Zapotoczna, Nabila Boksmati, Sinead Donohue, Baizurina Bahtiar, Ahmad Boland, Hamzah Al Somali, Alysia Cox, Hilary Humphreys, James P O'Gara, Marian Brennan, Eoghan O'Neill
PURPOSE: The treatment of device-related infections is challenging and current anti-microbial compounds have poor anti-biofilm activity. We aimed to identify and characterize novel compounds effective in the eradication of Staphylococcus aureus biofilms. METHODOLOGY: Two novel compounds, MMV665953 {1-(3-chloro-4-fluorophenyl)-3-(3,4-dichlorophenyl)urea} and MMV665807{5-chloro-2-hydroxy-N-[3-(trifluoromethyl)phenyl]benzamide}, effective in killing S. aureus biofilms, were identified by screening of the open access 'malaria box' chemical library...
March 23, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28314993/comparative-analysis-of-internalisation-haemolytic-cytotoxic-and-antibacterial-effect-of-membrane-active-cationic-peptides-aspects-of-experimental-setup
#3
Kata Horváti, Bernadett Bacsa, Tamás Mlinkó, Nóra Szabó, Ferenc Hudecz, Ferenc Zsila, Szilvia Bősze
Cationic peptides proved fundamental importance as pharmaceutical agents and/or drug carrier moieties functioning in cellular processes. The comparison of the in vitro activity of these peptides is an experimental challenge and a combination of different methods, such as cytotoxicity, internalisation rate, haemolytic and antibacterial effect, is necessary. At the same time, several issues need to be addressed as the assay conditions have a great influence on the measured biological effects and the experimental setup needs to be optimised...
March 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28299865/novel-short-antimicrobial-peptide-isolated-from-xenopus-laevis-skin
#4
Yu Zhang, Songcai Liu, Siming Li, Yunyun Cheng, Linyan Nie, Gang Wang, Chen Lv, Wenzhen Wei, Cheng Cheng, Feng Hou, Linlin Hao
A rich source of bioactive peptides, including a large number of antimicrobial peptides, has been found in amphibian skin. In this study, a novel short antimicrobial peptide was purified from Xenopus laevis skin and characterised through reversed-phase high-performance liquid chromatography, Edman degradation and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The peptide was composed of six amino acids with a sequence of DEDLDE and thus named X. laevis antibacterial peptide-P2 (XLAsp-P2)...
March 16, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28295642/residual-pyruvate-kinase-activity-in-pklr-deficient-erythroid-precursors-of-a-patient-suffering-from-severe-haemolytic-anaemia
#5
Thomas R L Klei, Sima Kheradmand Kia, Martijn Veldthuis, Boukje M Beuger, Judy Geissler, Javad Dehbozorgian, Mehran Karimi, Robin van Bruggen, Rob van Zwieten
OBJECTIVE: Here we present a 7 year old patient suffering from severe haemolytic anaemia. The most common cause of chronic hereditary non-spherocytic haemolytic anaemia is red blood cell pyruvate kinase (PK-R) deficiency. Since red blood cells rely solely on glycolysis to generate ATP, PK-R deficiency can severely impact energy supply and cause reduction of red blood cell lifespan. We determined the underlying cause of the anaemia and investigated why erythroid precursors in the patient survive...
March 10, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/28294414/evaluation-of-phytochemicals-from-medicinal-plants-of-myrtaceae-family-on-virulence-factor-production-by-pseudomonas-aeruginosa
#6
Khadar Syed Musthafa, Wipawadee Sianglum, Jongkon Saising, Sakkarin Lethongkam, Supayang Piyawan Voravuthikunchai
Virulence factors regulated by quorum sensing (QS) play a critical role in the pathogenesis of an opportunistic human pathogen, Pseudomonas aeruginosa in causing infections to the host. Hence, in the present work, the anti-virulence potential of the medicinal plant extracts and their derived phytochemicals from Myrtaceae family was evaluated against P. aeruginosa. In the preliminary screening of the tested medicinal plant extracts, Syzygium jambos and Syzygium antisepticum demonstrated a maximum inhibition in QS-dependent violacein pigment production by Chromobacterium violaceum DMST 21761...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28288581/prevalence-virulence-factor-genes-and-antibiotic-resistance-of-bacillus-cereus-sensu-lato-isolated-from-dairy-farms-and-traditional-dairy-products
#7
James Owusu-Kwarteng, Alhassan Wuni, Fortune Akabanda, Kwaku Tano-Debrah, Lene Jespersen
BACKGROUND: B. cereus are of particular interest in food safety and public health because of their capacity to cause food spoilage and disease through the production of various toxins. The aim of this study was to determine the prevalence, virulence factor genes and antibiotic resistance profile of B. cereus sensu lato isolated from cattle grazing soils and dairy products in Ghana. A total of 114 samples made up of 25 soil collected from cattle grazing farm land, 30 raw milk, 28 nunu (yoghurt-like product) and 31 woagashie (West African soft cheese)...
March 14, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28280630/the-role-of-chemotherapy-in-gastric-cancer-related-microangiopathic-haemolytic-anaemia
#8
Monica Tang, David Goldstein
We report a case of a previously well 46-year-old man who presented with microangiopathic haemolytic anaemia (MAHA) of unknown origin. After extensive investigations, he was diagnosed with cancer-related microangiopathic haemolytic anaemia (CR-MAHA) secondary to gastric adenocarcinoma. Initial treatment with plasmapheresis was ineffective, but the patient's haematological abnormalities improved markedly with chemotherapy directed against his gastric cancer. Our case amplifies previous experience of gastric cancer-associated MAHA which responded to treatment with chemotherapy...
February 2017: Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/28273806/antimicrobial-dendrimeric-peptides-structure-activity-and-new-therapeutic-applications
#9
REVIEW
Mariano A Scorciapino, Ilaria Serra, Giorgia Manzo, Andrea C Rinaldi
Microbial resistance to conventional antibiotics is one of the most outstanding medical and scientific challenges of our times. Despite the recognised need for new anti-infective agents, however, very few new drugs have been brought to the market and to the clinic in the last three decades. This review highlights the properties of a new class of antibiotics, namely dendrimeric peptides. These intriguing novel compounds, generally made of multiple peptidic sequences linked to an inner branched core, display an array of antibacterial, antiviral and antifungal activities, usually coupled to low haemolytic activity...
March 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28273152/pre-and-post-weaning-scours-in-southeastern-australia-a-survey-of-22-commercial-pig-herds-and-characterisation-of-escherichia-coli-isolates
#10
Lechelle K Van Breda, Om P Dhungyel, Andrew N Ginn, Jonathan R Iredell, Michael P Ward
Diarrhoeal diseases in piglets caused by Escherichia coli are responsible for substantial losses each year in the Australian pig industry. A cross-sectional survey was conducted (September 2013-May 2014) across 22 commercial pig herds located in southeastern Australia: NSW (n = 9); VIC (n = 10); and SA (n = 3), to estimate the prevalence of E. coli associated diarrhoea in pre- and post-weaned piglets and to identify key risk factors associated with E. coli disease. A questionnaire on management and husbandry practices was included...
2017: PloS One
https://www.readbyqxmd.com/read/28265787/isolation-and-characterisation-of-an-enterocin-p-producing-enterococcus-lactis-strain-from-a-fresh-shrimp-penaeus-vannamei
#11
Olfa Ben Braïek, Hamdi Ghomrassi, Paola Cremonesi, Stefano Morandi, Yannick Fleury, Patrick Le Chevalier, Khaled Hani, Omrane Bel Hadj, Taoufik Ghrairi
Screening for lactic acid bacteria (LAB) from fresh shrimp samples (Penaeus vannamei) collected from retail seafood markets in the Tunisian's coast, resulted in the isolation of an Enterococcus strain termed Q1. This strain was selected for its antagonistic activity against pathogenic bacteria such as Listeria monocytogenes, Pseudomonas aeruginosa, Lactococcus garvieae and against fungi (Aspergillus niger and Fusarium equiseti). The Q1 strain was characterised using standard morphological and biochemical tests, growth assays at different temperatures, pH and salinity...
March 7, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28263195/toenail-bracing-complicated-with-group-a-beta-haemolytic-streptococcus-infection
#12
Anca Chiriac, Cristina Birsan, Calin Molnar, Simona Stolnicu
No abstract text is available yet for this article.
March 2, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28262916/vitamin-e-supplementation-in-people-with-cystic-fibrosis
#13
REVIEW
Peter O Okebukola, Sonal Kansra, Joanne Barrett
BACKGROUND: People with cystic fibrosis are at an increased risk of fat-soluble vitamin deficiency including vitamin E. Vitamin E deficiency can cause a host of conditions such as haemolytic anaemia, cerebellar ataxia and cognitive difficulties. Vitamin E supplementation is widely recommended in cystic fibrosis and aims to ameliorate this deficiency. This is an updated version of the review. OBJECTIVES: To determine the effects of any level of vitamin E supplementation on the frequency of vitamin E deficiency disorders in people with cystic fibrosis...
March 6, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28256911/paroxysmal-nocturnal-haemoglobinuria-in-a-patient-with-primary-budd-chiari-syndrome-a-contraceptive-challenge
#14
Isabel Friedmann, Jacques Balayla
CASE REPORT: In this report, we describe the unique case of a 21 year-old woman, gravida 1, para 1, with paroxysmal nocturnal haemoglobinuria (PNH) and Budd-Chiari syndrome, as well as severe vaginismus and cervical stenosis, in need of contraception. Herein, we present the clinical considerations and implications taken to arrive at the right contraceptive choice for the patient. DISCUSSION: Budd-Chiari syndrome is defined by the presence of hepatic venous outflow tract obstruction, which may be due to a number of underlying causes...
April 2017: European Journal of Contraception & Reproductive Health Care
https://www.readbyqxmd.com/read/28254375/isolation-and-characterisation-of-a-novel-antibacterial-peptide-from-a-native-swine-intestinal-tract-derived-bacterium
#15
Haiyun Xin, Shengyue Ji, Jiayin Peng, Peng Han, Xiaopeng An, Shan Wang, Binyun Cao
Antimicrobial peptides (AMPs) are highly associated with antipathogenic activity, without generating drug resistance in targeted bacteria. In this study, the existence of AMPs in the Tibetan swine, a China-native, cold-resistant and seldom-sick breed of pig, was investigated. A peptide secreted by a Tibetan swine intestinal tract-derived Bacillus strain was isolated using reversed-phase chromatography (RPC), ultrafiltration and reversed-phase high-performance liquid chromatography (RP-HPLC). The peptide was identified by mass spectrometry and was characterised for activity against Escherichia coli and Staphylococcus aureus...
February 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28242109/haemolytic-uraemic-syndrome
#16
REVIEW
Fadi Fakhouri, Julien Zuber, Véronique Frémeaux-Bacchi, Chantal Loirat
Haemolytic uraemic syndrome is a form of thrombotic microangiopathy affecting predominantly the kidney and characterised by a triad of thrombocytopenia, mechanical haemolytic anaemia, and acute kidney injury. The term encompasses several disorders: shiga toxin-induced and pneumococcus-induced haemolytic uraemic syndrome, haemolytic uraemic syndrome associated with complement dysregulation or mutation of diacylglycerol kinase ɛ, haemolytic uraemic syndrome related to cobalamin C defect, and haemolytic uraemic syndrome secondary to a heterogeneous group of causes (infections, drugs, cancer, and systemic diseases)...
February 23, 2017: Lancet
https://www.readbyqxmd.com/read/28237973/modulatory-effects-of-melatonin-and-vitamin%C3%A2-%C3%A2-c-on-oxidative-stress-mediated-haemolytic-anaemia-and-associated-cardiovascular-dysfunctions-in-rats
#17
Temitayo Olabisi Ajibade, Ademola Adetokunbo Oyagbemi, Ladoke A Durotoye, Temidayo Olutayo Omóbòwálé, Ebunoluwa Racheal Asenuga, Funsho Olakitike Olayemi
Background Phenylhydrazine (PHE) in experimental animal models has been widely reported to cause haemolytic anaemia, via the induction of oxidative stress and thus causing deleterious cardiovascular complications. Hence, this study was designed to evaluate the possible modulatory role of melatonin (MLT) or vitamin C when co-administered with PHE. Methods Anaemia was established with PHE administration. MLT or vitamin C was co-administered with PHE. Haematological parameters, markers of oxidative stress, enzymic and non-enzymic antioxidants, blood pressure and electrocardiograms were assessed...
March 1, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28237662/the-derivatives-of-pulsatilla-saponin-a-a-bioactive-compound-from-pulsatilla-chinensis-their-synthesis-cytotoxicity-haemolytic-toxicity-and-mechanism-of-action
#18
Xiaohang Tong, Li Han, Huaqing Duan, Yaru Cui, Yulin Feng, Yongming Zhu, Zhong Chen, Shilin Yang
The strong haemolytic toxicity of Pulsatilla saponin A (PSA) has hampered its clinical development as an injectable anticancer agent. To circumvent this challenge, twenty PSA derivatives with C ring or C-28 or C-3 modifications were synthesized and evaluated for cytotoxicity against seven selected human tumor lines, as well as for haemolytic toxicity. Structure-activity relationship (SAR) and structure-toxicity relationship (STR) correlations were also elucidated. Compared with PSA, compound 22 showed a better balance between haemolytic toxicity (HD50 > 500 μM) and cytotoxicity toward lung cancer cells A549 (IC50 = 4...
March 31, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28236643/selection-of-indigenous-lactobacillus-paracasei-cd4-and-lactobacillus-gastricus-btm-7-as-probiotic-assessment-of-traits-combined-with-principal-component-analysis
#19
Kavita Sharma, Rishi Mahajan, Sampan Attri, Gunjan Goel
AIMS: The population of Himalayan region is known to consume variety of fermented and non fermented foods and as a result they have been benefited in terms of overall health, because of the associated beneficial microbes. Therefore, the focus of the present study was to identify new strains of Lactic Acid Bacteria (LAB) from dairy products such as milk (cow, goat, buffalo) and fermented products (curd and buttermilk) with properties suitable for use as probiotic cultures. METHODS AND RESULTS: A total of 75 isolates tentatively identified as LAB from 100 samples were initially screened for production of β-hemolysin as indicators of virulence which resulted in 38 isolates with no haemolytic activity...
February 25, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28216150/novel-biodegradable-poly-gamma-glutamic-acid-amphotericin-b-complexes-show-promise-as-improved-amphotericin-b-formulations
#20
T Dinh, Q Zia, S Zubair, P Stapleton, R Singh, M Owais, S Somavarapu
Commercially available amphotericin B (AmB) formulations are limited by cytotoxicities, lower efficacies, shelf-life related issues or high production costs. AmB complexes based on poly(gamma-glutamic acid) (PGGA) have been prepared and evaluated for their efficacies against AmB-deoxycholate (Fungizone®) and liposomal AmB (AmBisome®). Physical characterizations showed that AmB/PGGA complexes are nanoscopic (20-40 nm) with a negative zeta potential (-51.0 mV), water-soluble, stable in solution (up to 4 weeks, at 4 °C and 25 °C), and have a theoretical drug loading (up to 76...
February 16, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
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