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Maria I Dauden, Jan Kosinski, Olga Kolaj-Robin, Ambroise Desfosses, Alessandro Ori, Celine Faux, Niklas A Hoffmann, Osita F Onuma, Karin D Breunig, Martin Beck, Carsten Sachse, Bertrand Séraphin, Sebastian Glatt, Christoph W Müller
The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1-6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub-complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2, and Elp3 subunits form a two-lobed scaffold, which binds Elp456 asymmetrically...
February 2017: EMBO Reports
Osita N Okafor, K Farrington, Diana A Gorog
Epidemiological studies indicate that hyperuricaemia is an independent risk factor for cardiovascular disease. Alongside uric acid formation, increased xanthine oxidase activity also results in the formation of oxidative free radicals and superoxide particles. Oxidative stress significantly contributes to the development of cardiovascular disease, including endothelial cell dysfunction, atherosclerosis, vascular calcification and impaired myocardial energetics. Allopurinol, a competitive xanthine oxidase inhibitor, in addition to reducing serum uric acid levels, can act as a free radical scavenger...
December 2, 2016: Pharmacology & Therapeutics
Ukachukwu Okoroafor Abaraogu, Wisdom Onyedikachi Ugwa, Osita Nnodim, Elochukwu Fortune Ezenwankwo
BACKGROUND: The effects of backpack load on young adults' gait parameters have received little attention. We previously reported that with a backpack load within 15% body weight, young adults maintain their gait parameters at the expense of increased exertion during a 6-minute walk at self-selected normal walking speed. However, the effect of backpack strap pattern on gait parameters during faster walking or longer stride length has not been evaluated. OBJECTIVE: To determine the effect of different backpack strap patterns on gait parameters during a 12-minute walk test in young adults at self-selected normal and fast walking speeds...
October 22, 2016: PM & R: the Journal of Injury, Function, and Rehabilitation
Wilfred Sunday Ezema, Didacus Chukwuemeka Eze, Shodeinde Vincent Olu Shoyinka, John Osita Arinze Okoye
This project was undertaken to study the immunosuppressive capabilities of velogenic viscerotropic pathotype of Newcastle disease virus (VVNDV) infection in cockerels. Two hundred six-week-old cockerels were divided into four groups. Groups B/VUC and C/VC were vaccinated with LaSota in drinking water at 6 weeks of age. Groups C/VC and D/UC were challenged with VVNDV at 8 weeks of age. Three days post challenge (PC), the cockerels in group D/UC came down with clinical signs which included depression and greenish diarrhoea...
December 2016: Tropical Animal Health and Production
Osita I Onugha, Jay M Lee
In this chapter, we discuss the preoperative evaluation that is necessary prior to surgical resection, stage-specific surgical management of lung cancer, and the procedural steps as well as the indications to a variety of surgical approaches to lung resection.
2016: Cancer Treatment and Research
Sebastian Glatt, Rene Zabel, Olga Kolaj-Robin, Osita F Onuma, Florence Baudin, Andrea Graziadei, Valerio Taverniti, Ting-Yu Lin, Frauke Baymann, Bertrand Séraphin, Karin D Breunig, Christoph W Müller
During translation elongation, decoding is based on the recognition of codons by corresponding tRNA anticodon triplets. Molecular mechanisms that regulate global protein synthesis via specific base modifications in tRNA anticodons are receiving increasing attention. The conserved eukaryotic Elongator complex specifically modifies uridines located in the wobble base position of tRNAs. Mutations in Elongator subunits are associated with certain neurodegenerative diseases and cancer. Here we present the crystal structure of D...
September 2016: Nature Structural & Molecular Biology
Mohamed Farag, Maria Niespialowska-Steuden, Osita Okafor, Benjamin Artman, Manivannan Srinivasan, Arif Khan, Keith Sullivan, David Wellsted, Diana A Gorog
Non-vitamin K antagonist oral anticoagulants (NOACs) reduce the risk of thromboembolism in patients with atrial fibrillation (AF). There has been no head-to-head comparison of the effect of these agents on ex vivo thrombotic and thrombolytic status. Enhanced platelet reactivity and impaired endogenous thrombolysis are risk factors for recurrent thrombotic events. We aimed to assess the comparative effect of NOACs and warfarin using an ex vivo test of thrombosis and thrombolysis. Eighty patients with newly diagnosed non-valvular AF were tested before, and after being established on apixaban (n = 20), dabigatran (n = 20), rivaroxaban (n = 20), or warfarin (n = 20)...
November 2016: Platelets
Godwin Chinedu Okpe, Wilfred Sunday Ezema, Shodeinde Vincent Olumuyiwa Shoyinka, John Osita Arinze Okoye
This project was undertaken to find ways of reducing mortalities and economic losses due to velogenic Newcastle disease (VND) in areas where the disease is enzootic. Four groups of cockerels of 44 birds each were used for this experiment. The birds in groups 1 and 2 received no dietary vitamin A supplementation, whereas groups 3 and 4 received 300 iu and 600 iu of vitamin A per kilogram of commercial feed, respectively, from 1 week of age till the end of the experiment. At 6 weeks of age, the birds in groups 2, 3 and 4 were inoculated intraocularly with a VND virus (duck/Nigeria/Plateau/Kuru/113/1991)...
October 2015: International Journal of Experimental Pathology
Ikechukwu Innocent Mbachu, Gerald Udigwe, Ikechebelu Joseph, Okonkwo John, Umeononihu Osita Samuel, Ugboaja Joseph, Mbachu Chioma Ngozi
BACKGROUND: Accurate HIV testing in pregnancy is critical to the prevention of mother to child transmission of HIV infection and linkages to other preventive strategies. AIMS AND OBJECTIVES: This study determined the sensitivity, specificity negative and positive predictive value of serial rapid testing of HIV among pregnant women in Nnewi, south east Nigeria. METHODOLOGY: This was a comparative descriptive study conducted over a 4-month period...
2015: BMC Research Notes
Obinna Ositadimma Oleribe, Olabisi Oladipo, Princess Osita-Oleribe, Chukwuemeka Nwachukwu, Frank Nkwopara, Ekei Ekom, Solomon Nwabuzor, Grace Iyalla, Kenneth Onyewuchi, Ayodotun Olutola, Okey Nwanyanwu, Peter Nsubuga
INTRODUCTION: With the first case of Human Immunodeficiency Virus infection/Acquired Immunodeficiency Syndrome (HIV/AIDS) identified in 1986, the management of HIV/AIDS in Nigeria has evolved through the years. The emergency phase of the HIV/AIDS program, aimed at containing the HIV/AIDS epidemic within a short time frame, was carried out by international agencies that built structures separate from hospitals' programs. It is imperative that Nigeria shifts from the previous paradigm to the concept of Commonization of HIV to achieve sustainability...
2014: Pan African Medical Journal
Osita N Okafor, Diana A Gorog
Most acute cardiovascular events are attributable to arterial thrombosis. Plaque rupture or erosion stimulates platelet activation, aggregation, and thrombosis, whilst simultaneously activating enzymatic processes that mediate endogenous fibrinolysis to physiologically maintain vessel patency. Interplay between these pathways determines clinical outcome. If proaggregatory factors predominate, the thrombus may propagate, leading to vessel occlusion. However, if balanced by a healthy fibrinolytic system, thrombosis may not occur or cause lasting occlusion...
April 28, 2015: Journal of the American College of Cardiology
Anaelechi J Onuegbu, Japhet M Olisekodiaka, John U Udo, Osita Umeononihu, Ubuoh K Amah, John E Okwara, Chidiadi Atuegbu
OBJECTIVE: To evaluate the serum C-reactive protein (CRP) and lipid profile in women with pre-eclampsia. MATERIALS AND METHODS: Thirty-five women with and 35 women without pre-eclampsia, who were in the 3rd trimester of pregnancy, were enrolled in this study. Weight in kilogrammes and height in metres were measured to calculate the mean body mass index (BMI) for each group. The diastolic and systolic blood pressures were measured. Lipid profile tests and serum CRP assay were done for all patients...
2015: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
Christopher Bismarck Eke, Bartholomew Friday Chukwu, Anthony Nnaemeka Ikefuna, Osita Uchenna Ezenwosu, Ifeoma Josephine Emodi
BACKGROUND: Body composition indices are widely used to evaluate growth and nutrition in children, particularly those with sickle cell anemia (SCA), who are known to have impaired growth, impaired skeletal maturation, and delayed puberty. The current study is aimed at determining the body composition of children with SCA. METHODS: Consecutive selection of SCA children aged 6 to 18 years, who served as subjects and their age and gender matched children with HbAA (controls), selected using multi-stage systematic sampling...
May 2015: Pediatric Hematology and Oncology
Osita Kingsley Ezeh, Kingsley Emwinyore Agho, Michael John Dibley, John Joseph Hall, Andrew Nicolas Page
OBJECTIVES: To identify common factors associated with post-neonatal, infant, child and under-5 mortality in Nigeria. DESIGN, SETTING AND PARTICIPANTS: A cross-sectional data of three Nigeria Demographic and Health Surveys (NDHS) for the years 2003, 2008 and 2013 were used. A multistage, stratified, cluster random sampling method was used to gather information on 63,844 singleton live-born infants of the most recent birth of a mother within a 5-year period before each survey was examined using cox regression models...
2015: BMJ Open
Osita Kingsley Ezeh, Kingsley Emwinyore Agho, Michael John Dibley, John Joseph Hall, Andrew Nicolas Page
BACKGROUND: In Nigeria, approximately 69% of households use solid fuels as their primary source of domestic energy for cooking. These fuels produce high levels of indoor air pollution. This study aimed to determine whether Nigerian children residing in households using solid fuels at <5 years of age were at higher risk of death. METHODS: The 2013 Nigeria Demographic and Health Survey data were analysed in Cox regression analyses to examine the effects of solid fuel use on deaths of children aged 0-28 days (neonatal), 1-11 months (post-neonatal), and 12-59 months (child)...
2014: Environmental Health: a Global Access Science Source
Young Ju Lee, Seung Jun Son, Jae-Seung Paick, Soo Woong Kim
PURPOSE: To evaluate the clinical usefulness of preoperative CT voiding cystourethrography (CT-VCUG) using 16-multidetector computed tomography for female urethral diverticula. MATERIALS AND METHODS: Preoperative CT-VCUG was performed in 15 consecutive patients who underwent urethral diverticulectomy from May 2004 to December 2012. The result of preoperative cystourethroscopy and surgical findings were recorded by a single surgeon and CT-VCUG findings including the location of osita were retrospectively reviewed by another urologist who was blinded to the surgical finding...
2014: PloS One
Osita K Ezeh, Kingsley E Agho, Michael J Dibley, John Hall, Andrew N Page
In Nigeria, approximately 109 million and 66 million people lack access to sanitation facilities and water, respectively. This study aimed to determine whether children under 5 years old without access to improved water and sanitation facilities are at higher risk of death in Nigeria. Pooled 2003, 2008 and 2013 Nigeria Demographic and Health Survey data were used to examine the impact of water and sanitation on deaths of children aged 0-28 days, 1-11 months, and 12-59 months using Cox regression analysis. Survival information of 63,844 children was obtained, which included 6285 deaths of children under 5 years old; there were 2254 cases of neonatal mortality (0-28 days), 1859 cases of post-neonatal mortality (1-11 months) and 2,172 cases of child mortality (1-4 years old)...
September 2014: International Journal of Environmental Research and Public Health
George Uchenna Eleje, Anthony Osita lgwegbe, John E N Okonkwo, Gerald O Udigwe, Ahizechukwu Chigoziem Eke
BACKGROUND: The elderly primigravida is defined as a woman who goes into pregnancy for the first time at the age of 35 years or older. Progressively, this has become more common in our contemporary society and traditionally such pregnancy is regarded as high risk. OBJECTIVE: This was to determine and compare the pregnancy outcomes in elderly primigravidae aged 35 years and above with those of young primigravidae aged 20-25 years METHODS: The pregnancy outcomes of 82 elderly primigravidae (study group) with a singleton gestation, who delivered in the Teaching Hospital from 1st July, 2005 to 30th June, 2010 were compared with those of 131 young primigravidae (control group) who delivered during the same period in the same hospital...
July 2014: Nigerian Journal of Medicine: Journal of the National Association of Resident Doctors of Nigeria
George Uchenna Eleje, Euzebus Chinonye Ezugwu, Dotun Ogunyemi, Lydia Ijeoma Eleje, Joseph Ifeanyichukwu Ikechebelu, Anthony Osita Igwegbe, John E Okonkwo, Okechukwu Christian Ikpeze, Gerald Okanandu Udigwe, Hyacinth Eze Onah, Betrand Obi Nwosu, Chukwuemeka Okwudili Ezeama, Eziamaka Pauline Ezenkwele
AIM: To determine accuracy and costs of placental α-microglobulin-1 (PAMG-1) test compared to standard clinical assessment (SCA) for diagnosing rupture of membranes (ROM). METHODS: A multicenter double-blind study of consecutive women with symptoms and signs of ROM in Nnamdi Azikiwe University Teaching Hospital, Nnewi and University of Nigeria Teaching Hospital, Enugu, both in south-east Nigeria using SCA for ROM and the PAMG-1 test was done. ROM was diagnosed if two out of three methods from SCA (pooling, positive nitrazine test or ferning) were present and confirmed post-delivery based on presence of any two of these clinical criteria: delivery in 48 h to 7 days, evidence of chorioamnionitis, membranes overtly ruptured at delivery and adverse perinatal outcomes strongly correlated with prolonged PROM...
January 2015: Journal of Obstetrics and Gynaecology Research
Osita Kingsley Ezeh, Kingsley Emwinyore Agho, Michael John Dibley, John Hall, Andrew Nicholas Page
BACKGROUND: Nigeria continues to have one of the highest rates of neonatal deaths in Africa. This study aimed to identify risk factors associated with neonatal death in Nigeria using the 2008 Nigeria Demographic and Health Survey (NDHS). METHODS: Neonatal deaths of all singleton live-born infants between 2003 and 2008 were extracted from the 2008 NDHS. The 2008 NDHS was a multi-stage cluster sample survey of 36,298 households. Of these households, survival information of 27,147 singleton live-borns was obtained, including 996 cases of neonatal mortality...
2014: BMC Public Health
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