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Hui Yu, JianHua Chang, Fang Liu, Qifeng Wang, YongMing Lu, ZhuanXu Zhang, Jiabing Shen, Qing Zhai, Xia Meng, Jialei Wang, Xun Ye
Lung cancer patients with anaplastic lymphoma kinase (ALK) rearrangements are candidates for targeted therapeutics. However, patients must be tested with a companion diagnostic assay to realize their ALK rearrangement status. We analyzed the publicly available E-GEOD-31210 microarray dataset and identified a non-coding RNA, sweyjawbu, which is strongly associated with ALK rearrangements. We validated these results using quantitative real-time PCR in an independent cohort consisting of 4 cell lines and 83 clinical samples...
December 10, 2016: Oncotarget
C N Murphy, R C Fowler, P C Iwen, P D Fey
The BioFire FilmArray® Gastrointestinal Panel (GIP) was implemented to replace traditional stool culture and enzyme immunoassay (EIA) testing for stool pathogens. The purpose of this study was to evaluate the detection rate, incidence of coinfection, and culture recovery rate of gastrointestinal (GI) pathogens detected by the GIP over a 1-year period. A total of 2257 stools collected from January to December 2015 were tested using the GIP. Clostridium difficile colonization was also evaluated by an antigen/toxin EIA and confirmatory polymerase chain reaction (PCR)...
December 12, 2016: European Journal of Clinical Microbiology & Infectious Diseases
John M Humphrey, Sanjay Ranbhise, Emad Ibrahim, Hamad E Al-Romaihi, Elmoubasher Farag, Laith J Abu-Raddad, Marshall J Glesby
The causes of infectious diarrhea among the migrant worker population in Qatar are not well understood. We conducted a prospective observational study to understand the demographic and clinical characteristics and infectious causes of diarrhea among migrant workers in Doha, Qatar. A total of 126 male workers presenting to the Qatar Red Crescent Worker's Health Center outpatient clinic or emergency department were studied over a 5-month period in 2015-2016. Epidemiologic surveys were administered to all subjects and the prevalence of 22 different stool pathogens was determined using multiplex polymerase chain reaction (PCR) (FilmArray(®) Gastrointestinal PCR)...
December 7, 2016: American Journal of Tropical Medicine and Hygiene
Cristian Launes, Didac Casas-Alba, Claudia Fortuny, Ana Valero-Rello, Maria Cabrerizo, Carmen Muñoz-Almagro
No abstract text is available yet for this article.
January 2017: Journal of Clinical Microbiology
Erin H Graf, Maria Victoria Farquharson, Ana María Cárdenas
We compared an FDA cleared molecular meningitis/encephalitis panel to lab developed viral PCRs and bacterial culture. Of the 67 viral PCR or bacterial culture-positive samples, 92.5% were positive for the same target by the panel. Of the 66 negative samples tested, no targets were detected by the panel, for an agreement of 96.2%.
January 2017: Diagnostic Microbiology and Infectious Disease
Richard S P Huang, Coreen L Johnson, Lauryn Pritchard, Richard Hepler, Trang T Ton, James J Dunn
BACKGROUND: Multiplex syndromic panels have the capability of identifying causes of diarrheal illness. This study evaluated the performance characteristics of three multiplex molecular assays for the detection of common stool pathogens. METHODS: A total of 152 stool specimens were tested using three platforms: Verigene Enteric Pathogens Test (Verigene), Biofire FilmArray Gastrointestinal Panel (Biofire) and Luminex xTAG® Gastrointestinal Pathogen Panel (Luminex)...
December 2016: Diagnostic Microbiology and Infectious Disease
Emi Minejima, Annie Wong-Beringer
Antimicrobial stewardship (ASP) is an intervention-based program to improve patient outcomes to infection while limiting spread of resistance and unintended consequences. Many rapid diagnostic tools are now FDA cleared for clinical use, with three evaluated across multiple settings: Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, Verigene, and FilmArray. Areas covered: This review will focus on studies published that evaluated ASP intervention with rapid diagnostic implementation on outcomes of infection...
November 2016: Expert Review of Anti-infective Therapy
Frederick S Nolte, Lori Gauld, Susan B Barrett
We compared two rapid, point-of care nucleic acid amplification tests for detection of influenza A and B viruses (Alere i [Alere] and cobas Liat [Roche Diagnostics]) with the influenza A and B virus test components of the FilmArray respiratory panel (BioFire Diagnostics) using 129 respiratory specimens collected in universal viral transport medium (80 influenza A virus and 16 influenza B virus positive) from both adult and pediatric patients. The sensitivities of the Alere test were 71.3% for influenza A virus and 93...
November 2016: Journal of Clinical Microbiology
Kevin Messacar, Amanda L Hurst, Jason Child, Kristen Campbell, Claire Palmer, Stacey Hamilton, Elaine Dowell, Christine C Robinson, Sarah K Parker, Samuel R Dominguez
KEY POINTS: Real-time antimicrobial stewardship decision support for rapid blood culture diagnostics is associated with improved times to optimal and effective antimicrobial therapies and decreased unnecessary antimicrobial use in children. The unsolicited intervention incorporated with result reporting was well accepted and resulted in high provider satisfaction. BACKGROUND: Rapid diagnostic technologies for infectious diseases have the potential to improve clinical outcomes, but guideline-recommended antimicrobial stewardship (AS) strategies are not currently optimized for rapid intervention...
August 19, 2016: Journal of the Pediatric Infectious Diseases Society
Athanasios Michos, Alexandra Palili, Emmanouil I Koutouzis, Adina Sandu, Lilia Lykopoulou, Vassiliki P Syriopoulou
We report the use of FilmArray Blood Culture Identification (BCID) multiplex PCR system for pathogen detection from a child with septic arthritis that Streptococcus pyogenes was identified directly from synovial fluid and a child with complicated pneumonia with pleural effusion that Streptococcus pneumoniae was identified from pleural fluid.
2016: IDCases
Kimberly E Hanson
Rapid and accurate molecular diagnostic tests for the most common causes of infectious meningitis and encephalitis have the potential for high clinical impact. In this issue of the Journal of Clinical Microbiology, Leber et al. (J Clin Microbiol 54:2251-2261, 2016, report results from a large clinical study designed to prospectively assess the performance of the FilmArray meningitis/encephalitis panel compared to conventional methods.
September 2016: Journal of Clinical Microbiology
David Gilbert, Gita Gelfer, Lian Wang, Jillian Myers, Kristina Bajema, Michael Johnston, James Leggett
Two diagnostic bundles were compared in 127 evaluable patients admitted with community-acquired pneumonia (CAP). Diagnostic modalities in all patients included cultures of sputum (if obtainable) and blood, urine for detection of the antigens of Streptococcus pneumoniae and Legionella pneumophila, and nasal swabs for PCR probes for S. pneumoniae and Staphylococcus aureus. At least one procalcitonin level was measured in all patients. For virus detection, patients were randomized to either a 5-virus, lab-generated PCR panel or the broader and faster FilmArray PCR panel...
September 2016: Diagnostic Microbiology and Infectious Disease
Kevin Messacar, Garrett Breazeale, Christine C Robinson, Samuel R Dominguez
The FilmArray Meningitis Encephalitis Panel, a multiplex PCR for testing of cerebrospinal fluid, was compared to conventional diagnostic methods in children with suspected central nervous system infections. The panel had comparable diagnostic yield (96% agreement) and improved time-to-diagnosis by 10.3 hours with potential for more judicious antimicrobial use, particularly acyclovir.
September 2016: Diagnostic Microbiology and Infectious Disease
Amy L Leber, Kathy Everhart, Joan-Miquel Balada-Llasat, Jillian Cullison, Judy Daly, Sarah Holt, Paul Lephart, Hossein Salimnia, Paul C Schreckenberger, Sharon DesJarlais, Sharon L Reed, Kimberle C Chapin, Lindsay LeBlanc, J Kristie Johnson, Nicole L Soliven, Karen C Carroll, Jo-Anne Miller, Jennifer Dien Bard, Javier Mestas, Matthew Bankowski, Tori Enomoto, Andrew C Hemmert, Kevin M Bourzac
Rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. Comprehensive testing of cerebrospinal fluid (CSF) often includes Gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. These methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. The FilmArray Meningitis/Encephalitis (ME) Panel is a multiplexed in vitro diagnostic test for the simultaneous, rapid (∼1-h) detection of 14 pathogens directly from CSF specimens: Escherichia coli K1, Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus agalactiae, cytomegalovirus, enterovirus, herpes simplex virus 1 and 2, human herpesvirus 6, human parechovirus, varicella-zoster virus, and Cryptococcus neoformans/Cryptococcus gattii We describe a multicenter evaluation of 1,560 prospectively collected CSF specimens with performance compared to culture (bacterial analytes) and PCR (all other analytes)...
September 2016: Journal of Clinical Microbiology
Joseph J Carreno, Ben M Lomaestro, Apryl L Jacobs, Rachel E Meyer, Ann Evans, Clemente I Montero
OBJECTIVE To evaluate time to clinical response before and after implementation of rapid blood culture identification technologies. DESIGN Before-and-after trial. SETTING Large, tertiary, urban, academic health-sciences center. PATIENTS Patients >18 years old with sepsis and concurrent bacteremia or fungemia were included in the study; patients who were pregnant, had polymicrobial septicemia, or were transferred from an outside hospital were excluded. INTERVENTION Prior to the intervention, polymerase chain reaction was used to identify Staphylococcus species from positive blood cultures, and traditional laboratory techniques were used to identify non-staphylococcal species...
August 2016: Infection Control and Hospital Epidemiology
K De Rauw, L Detemmerman, J Breynaert, D Piérard
The purpose of this investigation was the evaluation of the performance of the BioFire FilmArray® Gastrointestinal (FA-GI) Panel, a multiplexed molecular stool screening assay, for the detection of diarrheagenic Escherichia coli (DEC), with emphasis on Shiga toxin-producing E. coli (STEC). A dilution series of 12 STEC reference strains was tested with the FA-GI Panel to assess the analytical sensitivity. A total of 389 patient samples were analyzed with the FA-GI Panel and confirmation of the detected DEC was attempted with an in-house culture-based polymerase chain reaction (PCR) method...
September 2016: European Journal of Clinical Microbiology & Infectious Diseases
Urania Rappo, Audrey N Schuetz, Stephen G Jenkins, David P Calfee, Thomas J Walsh, Martin T Wells, James P Hollenberg, Marshall J Glesby
Rapid and definitive diagnosis of viral respiratory infections is imperative in patient triage and management. We compared the outcomes for adult patients with positive tests for respiratory viruses at a tertiary care center across two consecutive influenza seasons (winters of 2010-2011 and 2012). Infections were diagnosed by conventional methods in the first season and by multiplex PCR (FilmArray) in the second season. FilmArray decreased the time to diagnosis of influenza compared to conventional methods (median turnaround times of 1...
August 2016: Journal of Clinical Microbiology
Shawn H MacVane, John M Hurst, M Sean Boger, John W Gnann
BACKGROUND: Early appropriate antibiotic selection is associated with favorable clinical outcomes. We evaluated the clinical impact of rapid detection of vancomycin-resistant Enterococcal bacteremia (VREB) by the FilmArray blood culture identification (BCID) panel coupled with antimicrobial stewardship program (ASP) interventions. METHODS: Hospitalized adult patients with VREB identified by conventional methods (CM) were compared to patients with VREB identified by BCID...
October 2016: Infectious Diseases
Morgan H McCoy, Ryan F Relich, Thomas E Davis, Bryan H Schmitt
Utilization of commercially available rapid platforms for microbial identification from positive blood cultures is useful during periods of, or in laboratories with, limited expert staffing. We compared the results of the FilmArray® BCID Panel performed by non-expert technologists to those of conventional methods for organism identification performed by skilled microbiologists. Within 8 h of signalling positive by a continuous monitoring blood culture system, positive bottles were analysed by the FilmArray BCID Panel...
July 2016: Journal of Medical Microbiology
Chris Stockmann, Andrew T Pavia, Brad Graham, Mike Vaughn, Rob Crisp, Mark A Poritz, Stephanie Thatcher, E Kent Korgenski, Trenda Barney, Judy Daly, Margarita Rogatcheva
KEY POINTS: In the era of widespread rotavirus vaccine use, toxigenic Clostridium difficile, diarrheagenic Escherichia coli, and viruses (particularly norovirus) are commonly detected among children with infectious gastroenteritis in the United States by using a multipathogen molecular panel. BACKGROUND: Diarrheal diseases are a major cause of ambulatory care visits and hospitalizations among children. Because of overlapping signs and symptoms and expensive and inefficient testing methods, the etiology of pediatric diarrhea is rarely established...
May 4, 2016: Journal of the Pediatric Infectious Diseases Society
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