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https://www.readbyqxmd.com/read/29620789/infectious-meningitis-encephalitis-evaluation-of-a-rapid-and-fully-automated-multiplex-pcr-in-the-microbiological-diagnostic-workup
#1
Giulia Piccirilli, Angela Chiereghin, Liliana Gabrielli, Maddalena Giannella, Diego Squarzoni, Gabriele Turello, Silvia Felici, Caterina Vocale, Roberta Zuntini, Dino Gibertoni, Alberto Enrico Maraolo, Simone Ambretti, Tiziana Lazzarotto
Infectious diseases of the central nervous system (CNS) such as meningitis/encephalitis (ME) require rapid identification of causative pathogens for effective treatment. This study evaluated the analytical performance and clinical utility of a fully automated multiplex PCR test to improve the microbiological diagnostic workup of ME. Seventy-seven cerebrospinal fluid (CSF) samples from 77 patients with suspected ME were studied. The samples were tested by FilmArray™ (FA) ME Panel test and the results were compared with those obtained using conventional microbiological procedures (CMP)...
April 5, 2018: New Microbiologica
https://www.readbyqxmd.com/read/29593057/multicenter-evaluation-of-the-biofire-filmarray-respiratory-panel-2-for-the-detection-of-viruses-and-bacteria-in-nasopharyngeal-swab-samples
#2
Amy L Leber, Kathy Everhart, Judy A Daly, Aubrey Hopper, Amanda Harrington, Paul Schreckenberger, Kathleen McKinley, Matthew Jones, Kristen Holmberg, Bart Kensinger
The FilmArray® Respiratory Panel 2 (RP2) is a multiplex in vitro diagnostic test for the simultaneous and rapid (∼45 minutes) detection of 22 pathogens directly from nasopharyngeal swab (NPS) samples. It contains updated (and in some instances redesigned) assays that improve upon the FilmArray® Respiratory Panel (RP; version 1.7), with a faster run time. The organisms identified are adenovirus, coronavirus 229E, coronavirus HKU1, coronavirus NL63, coronavirus OC43, human metapneumovirus, human rhinovirus/enterovirus, influenza A, influenza A H1, influenza A H1-2009, influenza A H3, influenza B, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3, parainfluenza virus 4, respiratory syncytial virus, Bordetella pertussis , Chlamydia pneumoniae , and Mycoplasma pneumoniae...
March 28, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29571294/detection-of-neisseria-meningitidis-in-a-paediatric-patient-with-septic-arthritis-using-multiplexed-diagnostic-pcr-targeting-meningitis-encephalitis-me
#3
Donnchadh O'Sullivan, Barry Linnane, Amanda Mostyn, Nteimam Jonathan, Marie Lenihan, Nuala H O'Connell, Colum P Dunne
BACKGROUND: Neisseria meningitidis is associated with meningitis and septicemia. Septic meningococcal arthritis is relatively uncommon and its diagnosis associated with clinical and microbiological challenges. Early recognition and treatment is required to prevent joint destruction. PURPOSE: We describe a case of an eleven-year-old boy with septic arthritis and the first reported use of a multiplexed diagnostic PCR test, capable of simultaneous rapid detection of 14 pathogens directly from CSF samples, to determine presence of N...
March 23, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29563204/frequency-of-instrument-environment-and-laboratory-technologist-contamination-during-routine-diagnostic-testing-of-infectious-specimens
#4
Melanie L Yarbrough, Jennie H Kwon, Meghan A Wallace, Tiffany Hink, Angela Shupe, Victoria J Fraser, Erik R Dubberke, Carey-Ann D Burnham
Laboratory testing to support the care of patients with highly infectious diseases may pose a risk for laboratory workers. However, data on the risk of virus transmission during routine laboratory testing conducted using standard personal protective equipment (PPE) are sparse. Our objective was to measure laboratory contamination during routine analysis of patient specimens. Remnant specimens were spiked with the nonpathogenic MS2 bacteriophage at 1.0 x 107 PFU/mL and biomarker contamination was assessed using RT-PCR for MS2...
March 21, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29540454/one-year-in-the-life-of-a-rapid-syndromic-panel-for-meningitis-encephalitis-a-pediatric-tertiary-care-facility-s-experience
#5
Samia N Naccache, Maryann Lustestica, Margil Fahit, Javier Mestas, Jennifer Dien Bard
Early establishment of infectious process allows for expedited clinical management of meningitis and encephalitis. The FilmArray Meningitis/Encephalitis (FA-M/E) panel provides rapid detection of potential pathogens associated with encephalitis/meningitis in both immunocompetent and compromised patients. Here we conducted a one year review of the performance of the FA-M/E panel at a tertiary care children's hospital. 265 samples from 251 patients were tested. 87.25% (219/251) were negative, 9.96% (25/251) were positive for viral analytes and 3...
March 14, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29533172/field-trials-of-blood-culture-identification-filmarray-in-regional-australian-hospitals
#6
Nicole M Bzdyl, Nadezda Urosevic, Ben Payne, Ray Brockenshire, Michael McIntyre, Michael J Leung, Graham Weaire-Buchanan, Elizabeth Geelhoed, Timothy J J Inglis
PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories. METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure...
March 13, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29514935/the-rapid-molecular-detection-of-gastrointestinal-pathogens-and-its-role-in-antimicrobial-stewardship
#7
Şiran Keske, Burak Zabun, Kahraman Aksoy, Füsun Can, Erhan Palaoğlu, Önder Ergönül
Background: We aimed to detect the etiological agents of acute diarrhea by molecular gastrointestinal pathogen test (MGPT) and to assess its impact on antimicrobial stewardship program (ASP). Methods: This is a prospective, observational study and was conducted between 1 January 2015 and 30 June 2017. We included consequent patients, who had acute diarrhea. At the end of 2015, we implemented ASP in acute diarrhea and we compared the outcomes in pre-ASP and post-ASP. An FDA-cleared multiplexed gastrointestinal PCR panel system, BioFire filmArray (Idaho Technology, Salt Lake City, UT) which detects twenty pathogens in stool was used...
March 7, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29411208/stool-pcr-for-gastrointestinal-pathogens-in-patients-with-and-without-immune-mediated-intestinal-diseases
#8
Yael R Nobel, Jordan Axelrad, Suzanne K Lewis, Susan Whittier, Garrett Lawlor, Simon Lichtiger, Peter H R Green, Benjamin Lebwohl
BACKGROUND: Patients with celiac disease and inflammatory bowel disease, two immune-mediated luminal conditions, have higher rates of certain infections than healthy counterparts. The prevalence of many gastrointestinal infections in these patients, however, is unknown. AIMS: Using a novel clinical stool pathogen PCR test, we investigated the hypothesis that patients with celiac disease/inflammatory bowel disease had different distributions of diarrheal pathogens than other patients...
February 6, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29401338/a-tertiary-care-center-s-experience-with-novel-molecular-meningitis-encephalitis-diagnostics-and-implementation-with-antimicrobial-stewardship
#9
David Chang, Jason F Okulicz, Lindsey E Nielsen, Brian K White
Background: Novel molecular techniques, such as the Biofire FilmArray Meningitis/Encephalitis (ME) panel, are increasingly used to improve pathogen detection and time to detection (TtD). The Brooke Army Medical Center antibiotic stewardship program evaluated the impact of the ME panel on empiric antimicrobial usage. Methods: Negative ME panels were analyzed for days of therapy (DOT). The ME panel became available at Brooke Army Medical Center on January 1, 2016 and a retrospective chart review was performed on all hospitalized patients tested by ME panel through April 30, 2016...
January 1, 2018: Military Medicine
https://www.readbyqxmd.com/read/29394385/use-of-a-multiplex-dna-extraction-pcr-in-the-identification-of-pathogens-in-travelers-diarrhea
#10
Bradley A Connor, Marina Rogova, Olga Whyte
Background: Diarrhea is one of the most common ailments afflicting travelers with attack rates of 30-40% for medium to high-risk destinations. As travelers' diarrhea (TD) is syndromic and caused by a wide range of pathogens, including bacteria, parasites and viruses, multiplex deoxyribonucleic acid (DNA) extraction polymerase chain reaction (PCR) technology can be useful for determining the etiology of TD pathogens. Objective: The goal of this retrospective study was to produce clinically relevant and useful data on gastrointestinal illness related to travel identified by culture-independent methods of diagnosis-use of the multiplex DNA extraction PCR platform (BioFire FilmArray GI Panel) and to describe the use of this technology in detection of enteric pathogens...
January 1, 2018: Journal of Travel Medicine
https://www.readbyqxmd.com/read/29332209/the-rapid-diagnosis-of-viral-respiratory-tract-infections-and-its-impact-on-antimicrobial-stewardship-programs
#11
Şiran Keske, Önder Ergönül, Faik Tutucu, Doruk Karaaslan, Erhan Palaoğlu, Füsun Can
We aimed to describe the potential benefit of new rapid molecular respiratory tests (MRT) in decreasing inappropriate antibiotic use among the inpatients presenting with influenza-like illness (ILI). We included patients from inpatient and outpatient departments who had ILI and performed MRT between 1 January 2015 and 31 December 2016 in a 265-bed private hospital in Istanbul. At the end of 2015, we implemented antimicrobial stewardship including systematic use of MRT. Then, we compared our observations between the year 2015 and the year 2016...
April 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29316801/economic-analysis-of-rapid-multiplex-polymerase-chain-reaction-testing-for-meningitis-encephalitis-in-pediatric-patients
#12
Steve Duff, Rodrigo Hasbun, Christine C Ginocchio, Joan-Miquel Balada-Llasat, Louise Zimmer, Samuel A Bozzette
AIM: We assessed the possible economic impact of a rapid test in pediatric patients with suspected community-acquired meningitis/encephalitis. MATERIALS & METHODS: Modeling simulated diagnosis, clinical decisions, resource use/costs of standard of care (SOC) and two cerebrospinal fluid testing strategies using FilmArray® (FA), a US FDA-cleared system that provides results in approximately 1 h. RESULTS: Pathogens detected by FA caused approximately 75% of cases, 97% of which would be accurately diagnosed with FA...
January 10, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29279453/evaluation-of-the-filmarray-blood-culture-identification-panel-on-detection-of-pathogenic-microorganisms-in-positive-blood-cultures-the-first-clinical-report-in-japan
#13
Kyoichi Saito, Shiro Endo, Makoto Katsumi, Chiyuki Ishizawa, Yuko Fujikawa, Shinya Inomata, Masahiro Toyokawa, Mitsuo Kaku
FilmArray (FA) is a multiplex PCR-based desktop microbial detection system. The blood culture identification (BCID) panel is an adaptable panel for FA, which diagnoses sepsis and/or systemic infections by detecting 14 bacterial species, 4 bacterial genera, 1 bacterial family, 5 yeast species, and 3 antimicrobial resistance genes (mecA, Klebsiella pneumoniae carbapenemase [KPC], and vanA/B) in positive blood cultures within 1 h. We retrospectively evaluated the FA-BCID panel using 54 positive blood cultures, in which 57 bacterial and 3 yeast strains were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
March 22, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29259822/efficacy-of-a-bovine-colostrum-and-egg-based-intervention-in-acute-childhood-diarrhoea-in-guatemala-a-randomised-double-blind-placebo-controlled-trial
#14
James T Gaensbauer, Mario A Melgar, Diva M Calvimontes, Molly M Lamb, Edwin J Asturias, Ingrid L Contreras-Roldan, Samuel R Dominguez, Christine C Robinson, Stephen Berman
Background: Treatments for paediatric diarrhoeal disease are limited. We assessed the impact of a bovine colostrum and egg-based treatment designed to reduce diarrhoea duration through non-specific and pathogen-directed mechanisms in children. Methods: Randomised, double-blind, placebo-controlled trial of PTM202, derived from bovine colostrum and hyperimmune hen's egg on the duration of acute diarrhoeal disease in Guatemalan children. PTM202 contains specific immunoglobulins that target rotavirus, enterotoxigenic Escherichia coli , Shiga toxin-producing E...
2017: BMJ Global Health
https://www.readbyqxmd.com/read/29237784/low-yield-of-filmarray-gi-panel-in-hospitalized-patients-with-diarrhea-an-opportunity-for-diagnostic-stewardship-intervention
#15
Matthew M Hitchcock, Carlos A Gomez, Niaz Banaei
The FilmArray GI panel (BioFire Diagnostics, Salt Lake City, UT) is a multiplex, on-demand, sample-to-answer, real-time PCR assay for the syndromic diagnosis of infectious gastroenteritis that has become widely adopted and, in some instances, has replaced conventional stool culture and parasite exams. Conventional testing has historically been restricted among hospitalized patients due to low diagnostic yield, but it is not known whether use of the FilmArray GI panel should be circumscribed. Cary-Blair stool samples submitted for FilmArray GI panel in adult patients admitted to an academic hospital from August 2015 to January 2017 were included in this study...
March 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29212701/multicenter-evaluation-of-the-eplex-respiratory-pathogen-panel-for-the-detection-of-viral-and-bacterial-respiratory-tract-pathogens-in-nasopharyngeal-swabs
#16
N Esther Babady, Matthew R England, Kristen L Jurcic Smith, Taojun He, Dona Saumya Wijetunge, Yi-Wei Tang, Robin R Chamberland, Marilyn Menegus, Ella M Swierkosz, Robert C Jerris, Wallace Greene
The performance of the new ePlex Respiratory Pathogen (RP) panel (GenMark Diagnostics) for the simultaneous detection of 19 viruses (influenza A virus; influenza A H1 virus; influenza A 2009 H1 virus; influenza A H3 virus; influenza B virus; adenovirus; coronaviruses [HKU1, OC43, NL63, and 229E]; human rhinovirus/enterovirus; human metapneumovirus; parainfluenza viruses 1, 2, 3, and 4; and respiratory syncytial virus [RSV] [RSV subtype A and RSV subtype B]) and 2 bacteria ( Mycoplasma pneumoniae and Chlamydia pneumoniae ) was evaluated...
February 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29208560/multiplex-pcr-system-for-the-rapid-diagnosis-of-respiratory-virus-infection-systematic-review-and-meta-analysis
#17
REVIEW
H-S Huang, C-L Tsai, J Chang, T-C Hsu, S Lin, C-C Lee
OBJECTIVES: To provide a summary of evidence for the diagnostic accuracies of three multiplex PCR systems (mPCRs)-BioFire FilmArray RP (FilmArray), Nanosphere Verigene RV+ test (Verigene RV+) and Hologic Gen-Probe Prodesse assays-on the detection of viral respiratory infections. METHODS: A comprehensive search up to 1 July 2017 was conducted on Medline and Embase for studies that utilized FilmArray, Verigene RV+ and Prodesse for diagnosis of viral respiratory infections...
December 5, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29099868/evaluation-of-a-multiplex-panel-for-the-diagnosis-of-acute-infectious-diarrhea-in-immunocompromised-hematologic-patients
#18
Izaskun Alejo-Cancho, Francesc Fernández Avilés, Alicia Capón, Cristina Rodríguez, Josep Barrachina, Pilar Salvador, Mª Eugenia Valls, Miriam J Álvarez-Martínez, Yuliya Zboromyrska, Jordi Vila, Mª Ángeles Marcos
INTRODUCTION: Diarrhea is a frequent complication in hematologic patients, being an infectious cause frequently suspected. Rapid and accurate detection of gastrointestinal pathogens is vital in immunocompromised hosts. The aim of this study was to compare routine diagnostic methods versus a multiplex polymerase chain reaction (PCR) assay for the diagnosis of infectious diarrhea in immunocompromised hematologic patients. MATERIAL AND METHODS: We conducted a prospective observational study from March 2015 to January 2016 to compare conventional methods for the diagnosis of infectious diarrhea with FIlmArray GI Panel (BioFire-bioMérieux, France)...
2017: PloS One
https://www.readbyqxmd.com/read/29096535/impact-of-early-multiplex-filmarray-respiratory-pathogen-panel-rpp-assay-on-hospital-length-of-stay-in-pediatric-patients-younger-than-3-months-admitted-for-fever-or-sepsis-workup
#19
Cory McFall, Hossein Salimnia, Paul Lephart, Ronald Thomas, Eric McGrath
No abstract text is available yet for this article.
November 1, 2017: Clinical Pediatrics
https://www.readbyqxmd.com/read/29093106/a-gastrointestinal-pcr-panel-improves-clinical-management-and-lowers-health-care-costs
#20
Stacy G Beal, Elizabeth E Tremblay, Steven Toffel, Lymaries Velez, Kenneth H Rand
Conventional methods for the identification of gastrointestinal pathogens are time-consuming and expensive and have limited sensitivity. The aim of this study was to determine the clinical impact of a comprehensive molecular test, the BioFire FilmArray gastrointestinal (GI) panel, which tests for many of the most common agents of infectious diarrhea in approximately 1 h. Patients with stool cultures submitted were tested on the GI panel ( n = 241) and were compared with control patients ( n = 594) from the year prior...
January 2018: Journal of Clinical Microbiology
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