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https://www.readbyqxmd.com/read/29744957/mitochondrial-lineage-a2ah-found-in-a-pre-hispanic-individual-from-the-andean-region
#1
M G Russo, C B Dejean, S A Avena, V Seldes, P Ramundo
OBJECTIVES: The aim of this study was to contribute to the knowledge of pre-Hispanic Andean mitochondrial diversity by analyzing an individual from the archaeological site Pukara de La Cueva (North-western Argentina). The date of the discovery context (540 ± 60 BP) corresponds to the Regional Developments II period. METHODS: Two separate DNA extractions were performed from dentin powder of one tooth. HVR I was amplified by PCR from each extract in three overlapping fragments and the haplotype was determined by consensus among all obtained sequences...
May 10, 2018: American Journal of Human Biology: the Official Journal of the Human Biology Council
https://www.readbyqxmd.com/read/29742720/sacral-epiduroscopic-laser-decompression-for-complex-regional-pain-syndrome-after-lumbar-spinal-surgery-a-case-report
#2
Jae-Wook Jung, Yong Han Kim, Hyojoong Kim, Eunsu Kang, Hyunji Jo, Myoung Jin Ko
RATIONALE: CRPS after a lumbar surgery has symptoms that are similar to PSSS. However, standard criteria for distinguishing CRPS from PSSS do not exist. We present a case report of a 31-year-old female with CRPS symptoms after lumbar spinal surgery treated by performing SELD. PATIENT CONCERNS: This patient was referred to our pain clinic for left ankle pain. She received a lumbar discectomy for a herniated lumbar disc (L5/S1) but the pain was aggravated after surgery...
May 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29607481/ancient-dna-reveals-temporal-population-structure-within-the-south-central-andes-area
#3
M Gabriela Russo, Fanny Mendisco, Sergio A Avena, Cristian M Crespo, Valeria Arencibia, Cristina B Dejean, Verónica Seldes
OBJECTIVES: The main aim of this work was to contribute to the knowledge of pre-Hispanic genetic variation and population structure among the South-central Andes Area by studying individuals from Quebrada de Humahuaca, North-western (NW) Argentina. MATERIALS AND METHODS: We analyzed 15 autosomal STRs in 19 individuals from several archaeological sites in Quebrada de Humahuaca, belonging to the Regional Developments Period (900-1430 AD). Compiling autosomal, mitochondrial, and Y-chromosome data, we evaluated population structure and differentiation among eight South-central Andean groups from the current territories of NW Argentina and Peru...
April 1, 2018: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/29488861/trans-sacral-epiduroscopic-laser-decompression-versus-the-microscopic-open-interlaminar-approach-for-l5-s1-disc-herniation
#4
Seung-Kook Kim, Su-Chan Lee, Seung-Woo Park
CONTEXT/OBJECTIVE: Trans-sacral epiduroscopic laser decompression (SELD) is an alternative to microscopic open lumbar discectomy (OLD). SELD and OLD for L5-S1 lumbar disc herniation (LDH) have not been compared. We compared clinical results, including pain control, between SELD and OLD. DESIGN: Retrospective analysis. SETTING: Korean hospital. PARTICIPANTS: Eighty patients treated with SELD (n = 40) or microscopic OLD (n = 40) for L5-S1 LDH...
February 28, 2018: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/29226162/relationship-between-age-at-onset-of-symptoms-and-intraoperative-findings-in-hip-arthroscopic-surgery
#5
Carlos Suarez-Ahedo, Chengcheng Gui, Stephanie M Rabe, John P Walsh, Sivashankar Chandrasekaran, Benjamin G Domb
Background: Hip arthroscopic surgery is intended to treat multiple abnormalities in an effort to delay the progression to osteoarthritis, especially in young patients. However, the length of time in which patients experience joint pain before seeking a specialist for a diagnosis can delay hip preservation surgery and influence clinical outcomes. Purpose: To investigate the relationship between age at symptom onset and findings during hip arthroscopic surgery as well as outcomes after 2 years of clinical follow-up...
November 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29220129/damage-control-resuscitation
#6
William Selde
No abstract text is available yet for this article.
April 2017: JEMS: a Journal of Emergency Medical Services
https://www.readbyqxmd.com/read/29202859/complications-of-lumbar-disc-herniations-following-trans-sacral-epiduroscopic-lumbar-decompression-a-single-center-retrospective-study
#7
Seung-Kook Kim, Su-Chan Lee, Seung-Woo Park, Eun-Sang Kim
BACKGROUND: Trans-sacral epiduroscopic lumbar decompression (SELD) is an emerging procedure for the treatment of lumbar disc herniation (LDH), with favorable outcomes having been reported. However, the complications associated with SELD have not been comprehensively evaluated to date. Therefore, the aim of our study was to describe the incidence rate, types, and characteristics of complications following SELD and management outcomes. METHODS: Retrospective analysis of the surgical and clinical outcomes for 127 patients (average age, 42...
December 4, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29135288/selective-debridement-with-labral-preservation-using-narrow-indications-in-the-hip-minimum-5-year-outcomes-with-a-matched-pair-labral-repair-control-group
#8
Austin W Chen, Leslie C Yuen, Victor Ortiz-Declet, Jody Litrenta, David R Maldonado, Benjamin G Domb
BACKGROUND: Arthroscopic labral debridement in the hip can be an effective treatment for labral tears but has demonstrated inferior outcomes compared with labral repair. Thus, the role for labral debridement has become unclear. Hypothesis/Purpose: The purpose was to evaluate the outcomes of a selective debridement with labral preservation (SDLP) group with a minimum 5-year follow-up. It was hypothesized that, with narrow indications, SDLP would produce statistically improved patient-reported outcome (PRO) scores, comparable with those of a matched-pair labral repair control group...
February 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29116155/using-crispr-cas9-mediated-genome-editing-to-generate-c-difficile-mutants-defective-in-selenoproteins-synthesis
#9
Kathleen N McAllister, Laurent Bouillaut, Jennifer N Kahn, William T Self, Joseph A Sorg
Clostridium difficile is a significant concern as a nosocomial pathogen, and genetic tools are important when analyzing the physiology of such organisms so that the underlying physiology/pathogenesis of the organisms can be studied. Here, we used TargeTron to investigate the role of selenoproteins in C. difficile Stickland metabolism and found that a TargeTron insertion into selD, encoding the selenophosphate synthetase that is essential for the specific incorporation of selenium into selenoproteins, results in a significant growth defect and a global loss of selenium incorporation...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29049245/sacral-root-injury-during-trans-sacral-epiduroscopic-laser-decompression-a-case-report
#10
Min Cheol Chang
RATIONALE: Trans-sacral epiduroscopic laser decompression (SELD) is reported to have strong positive short-term effects in controlling lower back pain (LBP) or radicular leg pain following herniated lumbar disc (HLD). However, little is known about the possible complications following SELD. PATIENT CONCERNS: A 36-year-old man received SELD with YAG laser for controlling LBP and radicular pain due to HLD on L5-S1. However, after SELD, voiding and defecation difficulties and sensory deficits on the S3-5 dermatome were presented...
October 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28609125/minimum-2-year-outcomes-of-hip-arthroscopic-surgery-in-patients-with-acetabular-overcoverage-and-profunda-acetabulae-compared-with-matched-controls-with-normal-acetabular-coverage
#11
Sivashankar Chandrasekaran, Nader Darwish, Edwin O Chaharbakhshi, Carlos Suarez-Ahedo, Parth Lodhia, Benjamin G Domb
BACKGROUND: Advancements in instrumentation and techniques have extended the scope of hip arthroscopic surgery to treat complex osseous deformities that were previously best addressed with an open approach. Global pincer-type femoroacetabular impingement is an example of an abnormality requiring osseous correction with a technically challenging access point. PURPOSE: To report on the patterns of clinical presentation and intra-articular derangements, radiological associations, and minimum 2-year outcomes after hip arthroscopic surgery in patients with a lateral center edge angle (LCEA) >40° and profunda acetabulae in comparison with matched controls with normal acetabular coverage...
September 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27505931/into-the-wild
#12
Benjamin Abo, William Selde
No abstract text is available yet for this article.
June 2016: JEMS: a Journal of Emergency Medical Services
https://www.readbyqxmd.com/read/27505930/altitude-illness
#13
William Selde, Will Smith
No abstract text is available yet for this article.
June 2016: JEMS: a Journal of Emergency Medical Services
https://www.readbyqxmd.com/read/27346733/pre-hispanic-mortuary-practices-in-quebrada-de-humahuaca-north-western-argentina-genetic-relatedness-among-individuals-buried-in-the-same-grave
#14
M Gabriela Russo, Fanny Mendisco, Sergio A Avena, Cristina B Dejean, Verónica Seldes
Almost all pre-Hispanic societies from Quebrada de Humahuaca (north-western Argentina) buried their defuncts in domestic areas, demonstrating the importance of death and its daily presence among the living. Presumably, the collective graves contained related individuals, a hypothesis that can be tested through the study of ancient DNA. This study analyzes autosomal and uniparental genetic markers in individuals from two archaeological sites in Quebrada de Humahuaca occupied during the Late Formative (1450-1050 BP) and Regional Developments I (1050-700 BP) periods...
July 2016: Annals of Human Genetics
https://www.readbyqxmd.com/read/26901647/trans-sacral-epiduroscopic-laser-decompression-for-symptomatic-lumbar-disc-herniation-a-preliminary-case-series
#15
Sung Ho Lee, Sang-Ho Lee, Kang Taek Lim
OBJECTIVE: The purpose of this study was to investigate the effect of trans-sacral epiduroscopic laser decompression (SELD) in patients with a herniated lumbar disc. METHODS: This prospective case series study was designed to determine the outcomes of SELD with regard to reducing pain and improving the functional status in patients with low back pain (LBP) and radiculopathy caused by definitive neural compression confirmed by MRI. A total of 250 patients with LBP and simultaneous radiculopathy underwent SELD by applying a Ho:YAG laser...
March 2016: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/26857572/novel-two-step-hierarchical-screening-of-mutant-pools-reveals-mutants-under-selection-in-chicks
#16
Hee-Jeong Yang, Lydia M Bogomolnaya, Johanna R Elfenbein, Tiana Endicott-Yazdani, M Megan Reynolds, Steffen Porwollik, Pui Cheng, Xiao-Qin Xia, Michael McClelland, Helene Andrews-Polymenis
Contaminated chicken/egg products are major sources of human salmonellosis, yet the strategies used by Salmonella to colonize chickens are poorly understood. We applied a novel two-step hierarchical procedure to identify new genes important for colonization and persistence of Salmonella enterica serotype Typhimurium in chickens. A library of 182 S. Typhimurium mutants each containing a targeted deletion of a group of contiguous genes (for a total of 2,069 genes deleted) was used to identify regions under selection at 1, 3, and 9 days postinfection in chicks...
April 2016: Infection and Immunity
https://www.readbyqxmd.com/read/26443353/selenophosphate-synthetase
#17
Matt D Wolfe
Selenophosphate synthetase, the selD gene product from Escherichia coli, is one of the enzymes required for the synthesis and specific insertion of selenocysteine into proteins directed by the TGA codon. Selenophosphate synthetases have been isolated from or are thought to be present in most organisms; however, the best characterized selenophosphate synthetase is from E. coli, in which both in vivo and in vitro studies have been performed. Leinfelder and coworkers showed that an E. coli mutant lacking an intact selD gene fails to incorporate Se into both the selenocysteine-containing enzyme formate dehydrogenase (FDH) and tRNA species that normally contain 2-selenouridine residues at the wobble position...
December 2004: EcoSal Plus
https://www.readbyqxmd.com/read/26378233/formation-of-a-ternary-complex-for-selenocysteine-biosynthesis-in-bacteria
#18
Ivan R Silva, Vitor H B Serrão, Livia R Manzine, Lívia M Faim, Marco T A da Silva, Raphaela Makki, Daniel M Saidemberg, Marinônio L Cornélio, Mário S Palma, Otavio H Thiemann
The synthesis of selenocysteine-containing proteins (selenoproteins) involves the interaction of selenocysteine synthase (SelA), tRNA (tRNA(Sec)), selenophosphate synthetase (SelD, SPS), a specific elongation factor (SelB), and a specific mRNA sequence known as selenocysteine insertion sequence (SECIS). Because selenium compounds are highly toxic in the cellular environment, the association of selenium with proteins throughout its metabolism is essential for cell survival. In this study, we demonstrate the interaction of SPS with the SelA-tRNA(Sec) complex, resulting in a 1...
December 4, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26354305/air-medical-curricula-in-emergency-medicine-residencies-a-national-survey
#19
Joshua Mastenbrook, Paul Savino, Paul Mazurek, Jan Eichel, William Selde, Glenn Ekblad
OBJECTIVE: The aim of this study was to determine if a common air medical curriculum exists among Accreditation Council for Graduate Medical Education (ACGME) emergency medicine (EM) residencies. METHODS: Institutional review board exemption was obtained. A cross-sectional survey study design was used. All ACGME-approved EM residencies as of February 2013 were identified, and the program directors were e-mailed a 3-question survey. Descriptive statistics were calculated for each response, and a response bias analysis was completed...
September 2015: Air Medical Journal
https://www.readbyqxmd.com/read/26194102/evolution-of-selenophosphate-synthetases-emergence-and-relocation-of-function-through-independent-duplications-and-recurrent-subfunctionalization
#20
Marco Mariotti, Didac Santesmasses, Salvador Capella-Gutierrez, Andrea Mateo, Carme Arnan, Rory Johnson, Salvatore D'Aniello, Sun Hee Yim, Vadim N Gladyshev, Florenci Serras, Montserrat Corominas, Toni Gabaldón, Roderic Guigó
Selenoproteins are proteins that incorporate selenocysteine (Sec), a nonstandard amino acid encoded by UGA, normally a stop codon. Sec synthesis requires the enzyme Selenophosphate synthetase (SPS or SelD), conserved in all prokaryotic and eukaryotic genomes encoding selenoproteins. Here, we study the evolutionary history of SPS genes, providing a map of selenoprotein function spanning the whole tree of life. SPS is itself a selenoprotein in many species, although functionally equivalent homologs that replace the Sec site with cysteine (Cys) are common...
September 2015: Genome Research
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