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Fluorescence lung cancer

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https://www.readbyqxmd.com/read/28109303/uptake-of-dna-by-cancer-cells-without-a-transfection-reagent
#1
Yanping Kong, Xianbo Zhang, Yongliang Zhao, Yanfang Xue, Ye Zhang
BACKGROUND: Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the understanding of the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle to be transferred into cells; this process is called transfection in laboratory. Cancer cells are known for having gene mutations and the evolving ability of endocytosis...
January 21, 2017: Biological Research
https://www.readbyqxmd.com/read/28107959/use-of-quantum-dot-beads-labeled-monoclonal-antibody-to-improve-the-sensitivity-of-a-quantitative-and-simultaneous-immunochromatographic-assay-for-neuron-specific-enolase-and-carcinoembryonic-antigen
#2
Kun Xiao, Kan Wang, Weijian Qin, Yafei Hou, Wenting Lu, Hao Xu, Yan Wo, Daxiang Cui
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH-) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28106046/obp-401-gfp-telomerase-dependent-adenovirus-illuminates-and-kills-high-metastatic-more-effectively-than-low-metastatic-triple-negative-breast-cancer-in-vitro
#3
S Yano, K Takehara, H Kishimoto, H Tazawa, Y Urata, S Kagawa, M Bouvet, T Fujiwara, R M Hoffman
We previously described the development of a highly-invasive, triple-negative breast cancer (TNBC) variant using serial orthotopic implantation of MDA-MB-231 human breast cancer in nude mice. The isolated variant is highly invasive in the mammary gland and metastasized to lymph nodes in 10 of 12 mice compared with 2 of 12 of the parental cell line. OBP-401 is a telomerase-dependent cancer-specific, green fluorescent protein (GFP)-expressing adenovirus. OBP-401 was used to infect parental MDA-MB-231P cells and high-metastatic MDA-MB-231H and MDA-MB-231HLN isolated from a lymph node metastasis and MDA-MB-231HLM isolated from a lung metastasis...
January 20, 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28105185/lim-and-sh3-protein-1-promotes-tumor-proliferation-and-metastasis-in-lung-carcinoma
#4
Xueqiong Lin, Xianhui Liu, Yusen Fang, Xuefen Weng
Lung cancer is the most frequently diagnosed cancer and the leading cause of cancer-related mortality worldwide. In the present study, we focused on LIM and SH3 protein 1 (LASP-1), a key molecule involved in the development of multiple cancers, and attempted to elucidate its effect on the oncogenesis of lung cancer. We determined the expression level of LASP-1 in lung cancer using reverse transcription-quantitative polymerase chain reaction and western blot analysis, and also studied the potential function of LASP-1 in lung cancer cell growth, apoptosis and migration by small interfering RNA transfection...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105150/ovarian-metastasis-from-non-small-cell-lung-cancer-with-alk-and-egfr-mutations-a-report-of-two-cases
#5
Rosemary T Mushi, Yumei Yang, Qian Cai, Ruiguang Zhang, Gang Wu, Xiaorong Dong
Ovarian metastasis from non-small cell lung cancer (NSCLC) is a rare condition. The current study presents the cases of 2 female patients aged 38 and 47 years old, respectively, who were initially diagnosed with NSCLC adenocarcinoma on histology. Both patients initially presented with chest pain and a cough, and subsequently developed ovarian metastases following multiple treatments. The 38-year old patient exhibited an epidermal growth factor receptor mutation, confirmed by scorpion/amplified refractory mutation system analysis from a lung biopsy...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28103936/elevated-collagen-i-augments-tumor-progressive-signals-intravasation-and-metastasis-of-prolactin-induced-estrogen-receptor-alpha-positive-mammary-tumor-cells
#6
Craig E Barcus, Kathleen A O'Leary, Jennifer L Brockman, Debra E Rugowski, Yuming Liu, Nancy Garcia, Menggang Yu, Patricia J Keely, Kevin W Eliceiri, Linda A Schuler
BACKGROUND: The development and progression of estrogen receptor alpha positive (ERα+) breast cancer has been linked epidemiologically to prolactin. However, activation of the canonical mediator of prolactin, STAT5, is associated with more differentiated cancers and better prognoses. We have reported that density/stiffness of the extracellular matrix potently modulates the repertoire of prolactin signals in human ERα + breast cancer cells in vitro: stiff matrices shift the balance from the Janus kinase (JAK)2/STAT5 cascade toward pro-tumor progressive extracellular regulated kinase (ERK)1/2 signals, driving invasion...
January 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28098887/mirna%C3%A2-375-regulates-the-cell-survival-and-apoptosis-of-human-non%C3%A2-small-cell-carcinoma-by-targeting-her2
#7
Longqiang Cheng, Bingxiang Zhan, Peng Luo, Baolong Wang
micro (mi)‑RNAs are a class of small non‑coding RNAs that regulate gene expression by binding to the 3'‑untranslated region of mRNA, which may lead to mRNA degradation or transcription regulation. Previous studies indicated that miRNAs are important for the pathogenesis of human cancer. miR‑375 has been implicated in various tumor types; however, the biological activity in human non‑small cell lung carcinoma (NSCLC) cells remains to be fully elucidated. The purpose of the present study was to investigate the biological importance of miR‑375 in human NSCLC cells...
January 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28088493/apoptosis-induced-by-the-methanol-extract-of-salvia-miltiorrhiza-bunge-in-non-small-cell-lung-cancer-through-pten-mediated-inhibition-of-pi3k-akt-pathway
#8
Yin-Tao Ye, Wei Zhong, Pei Sun, Dong Wang, Chen Wang, Li-Min Hu, Jun-Qiang Qian
ETHNOPHARMACOLOGICAL RELEVANCE: Salvia miltiorrhiza Bunge, a well-known traditional Chinese medicinal (TCM) plant, has been used to treat cardiovascular diseases since thousands of years. Many studies have reported that the active component tanshinones displayed a variety of biological activities: antithrombous, antiplatelet aggregation, antioxidant and antitumor promoting. But the mechanism of how the active components working still need to be clarified. The anti-tumor effect of compounds of tanshinone (CTN), the methanol extract of Salvia miltiorrhiza Bunge roots, was investigated...
January 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28069800/label-free-raman-spectroscopy-detects-stromal-adaptations-in-premetastatic-lungs-primed-by-breast-cancer
#9
Santosh Kumar Paidi, Asif Rizwan, Chao Zheng, Menglin Cheng, Kristine Glunde, Ishan Barman
Recent advances in animal modeling, imaging technology, and functional genomics have permitted precise molecular observations of the metastatic process. However, a comprehensive understanding of the premetastatic niche remains elusive, owing to the limited tools that can map subtle differences in molecular mediators in organ-specific microenvironments. Here, we report the ability to detect premetastatic changes in the lung microenvironment, in response to primary breast tumors, using a combination of metastatic mouse models, Raman spectroscopy, and multivariate analysis of consistent patterns in molecular expression...
January 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28068967/enterolactone-alters-fak-src-signaling-and-suppresses-migration-and-invasion-of-lung-cancer-cell-lines
#10
Shireen Chikara, Kaitlin Lindsey, Pawel Borowicz, Melpo Christofidou-Solomidou, Katie M Reindl
BACKGROUND: Systemic toxicity of chemotherapeutic agents and the challenges associated with targeting metastatic tumors are limiting factors for current lung cancer therapeutic approaches. To address these issues, plant-derived bioactive components have been investigated for their anti-cancer properties because many of these agents are non-toxic to healthy tissues. Enterolactone (EL) is a flaxseed-derived mammalian lignan that has demonstrated anti-migratory properties for various cancers, but EL has not been investigated in the context of lung cancer, and its anticancer mechanisms are ill-defined...
January 9, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28066672/intraoperative-molecular-imaging-to-identify-lung-adenocarcinomas
#11
REVIEW
Andrew D Newton, Gregory T Kennedy, Jarrod D Predina, Philip S Low, Sunil Singhal
Intraoperative molecular imaging is a promising new technology with numerous applications in lung cancer surgery. Accurate identification of small nodules and assessment of tumor margins are two challenges in pulmonary resections for cancer, particularly with increasing use of video-assisted thoracoscopic surgery (VATS). One potential solution to these problems is intraoperative use of a fluorescent contrast agent to improve detection of cancer cells. This technology requires both a targeted fluorescent dye that will selectively accumulate in cancer cells and a specialized imaging system to detect the cells...
October 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28065229/detection-of-echinoderm-microtubule-associated-protein-like-4-anaplastic-lymphoma-kinase-fusion-genes-in-non-small-cell-lung-cancer-clinical-samples-by-a-real-time-quantitative-reverse-transcription-polymerase-chain-reaction-method
#12
Jing Zhao, Jin-Yin Zhao, Zhi-Xia Chen, Wei Zhong, Long-Yun Li, Li-Cheng Liu, Xiao-Xu Hu, Wei-Jun Chen, Meng-Zhao Wang
Objective To establish a real-time quantitative reverse transcription polymerase chain reaction assay (qRT-PCR) for the rapid, sensitive, and specific detection of echinoderm microtubule associated protein like 4-anaplastic lymphoma kinase (EML4-ALK) fusion genes in non-small cell lung cancer. Methods The specific primers for the four variants of EML4-ALK fusion genes (V1, V2, V3a, and V3b) and Taqman fluorescence probes for the detection of the target sequences were carefully designed by the Primer Premier 5...
December 20, 2016: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/28054838/il-6-mediates-macrophage-infiltration-after-irradiation-via-up-regulation-of-ccl2-ccl5-in-non-small-cell-lung-cancer
#13
Xin Wang, Xiaodong Yang, Ying Tsai, Li Yang, Kuang-Hsiang Chuang, Peter C Keng, Soo Ok Lee, Yuhchyau Chen
Radiotherapy is effective in reducing primary tumors, however, it may enhance macrophage infiltration to tumor sites, accelerating tumor progression in several ways. We investigated whether radiation can increase macrophage infiltration into non-small cell lung carcinoma (NSCLC) cells. Analysis of in vitro macrophage (differentiated THP-1 cells) migration to either nonirradiated or irradiated tumor cells showed increased migration to the irradiated tumor cells. Because the IL-6 levels in A549 and H157 cells were significantly increased after irradiation, we then investigated whether this increased IL-6 level contributes to radiation-induced macrophage migration...
January 5, 2017: Radiation Research
https://www.readbyqxmd.com/read/28046379/we-ab-bra-01-biodistribution-of-paclitaxel-loaded-nanodroplets-for-prostate-cancer-management-using-ultrasound-mediated-drug-delivery-under-mrguidance
#14
R Gupta, D Cvetkovic, B Wang, L Chen, C Ma
PURPOSE: To study the biodistribution of paclitaxel-loaded nanodroplets in vivo in order to evaluate the efficacy of focused ultrasound (FUS)-mediated drug delivery under MR-guidance to prostate tumor. METHODS: Poly {ethylene oxide}-co-poly {D, L-lactide} (PDLA) nanodroplets loaded with fluorescently labeled-paclitaxel (F-PTX) were synthesized using solid dispersion technique. Human prostate cancer, LNCaP cells were implanted orthotopically in prostates of male nude mice...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28045514/specific-conformational-change-in-giant-dna-caused-by-anticancer-tetrazolato-bridged-dinuclear-platinum-ii-complexes-middle-length-alkyl-substituents-exhibit-minimum-effect
#15
Seiji Komeda, Hiroki Yoneyama, Masako Uemura, Akira Muramatsu, Naoto Okamoto, Hiroaki Konishi, Hiroyuki Takahashi, Akimitsu Takagi, Wakao Fukuda, Tadayuki Imanaka, Toshio Kanbe, Shinya Harusawa, Yuko Yoshikawa, Kenichi Yoshikawa
Derivatives of the highly antitumor-active compound [{cis-Pt(NH3)2}2(μ-OH)(μ-tetrazolato-N2,N3)](2+) (5-H-Y), which is a tetrazolato-bridged dinuclear platinum(II) complex, were prepared by substituting a linear alkyl chain moiety at C5 of the tetrazolate ring. The general formula for the derivatives is [{cis-Pt(NH3)2}2(μ-OH)(μ-5-R-tetrazolato-N2,N3)](2+), where R is (CH2)nCH3 and n = 0 to 8 (complexes 1-9). The cytotoxicity of complexes 1-4 in NCI-H460 human non-small-cell lung cancer cells decreased with increasing alkyl chain length, and those of complexes 5-9 increased with increasing alkyl chain length...
January 3, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28043076/bio-sniffer-gas-phase-biosensor-with-secondary-alcohol-dehydrogenase-s-adh-for-determination-of-isopropanol-in-exhaled-air-as-a-potential-volatile-biomarker
#16
Po-Jen Chien, Takuma Suzuki, Masato Tsujii, Ming Ye, Koji Toma, Takahiro Arakawa, Yasuhiko Iwasaki, Kohji Mitsubayashi
Exhaled breath analysis has attracted lots of researchers attention in the past decades due to its advantages such as its non-invasive property and the possibility of continuous monitoring. In addition, several volatile organic compounds in breath have been identified as biomarkers for some diseases. Particularly, studies have pointed out that concentration of isopropanol (IPA) in exhaled air might relate with certain illnesses such as liver disease, chronic obstructive pulmonary (COPD), and lung cancer. In this study, a highly sensitive and selective biochemical gas sensor (bio-sniffer) for the breath IPA concentration determination was constructed and optimized...
December 22, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28042036/pharmacological-characterization-of-synthetic-serine-palmitoyltransferase-inhibitors-by-biochemical-and-cellular-analyses
#17
Ryutaro Adachi, Yasutomi Asano, Kazumasa Ogawa, Motomi Oonishi, Yukiya Tanaka, Tomohiro Kawamoto
Human serine palmitoyltransferase (SPT) is a PLP-dependent enzyme residing in the endoplasmic reticulum. It catalyzes the synthesis of 3-ketodihydrosphingosine (3-KDS) from the substrates palmitoyl-CoA and L-serine. It is a rate-limiting enzyme for sphingolipid synthesis in cells. In the present study, we characterized and pharmacologically profiled a series of tetrahydropyrazolopyridine derivatives that potently inhibit human SPT enzymatic activity, including two cell-active derivatives and one fluorescent-labelled derivative...
December 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28039472/analysis-of-circulating-tumor-cells-from-lung-cancer-patients-with-multiple-biomarkers-using-high-performance-size-based-microfluidic-chip
#18
Wanlei Gao, Haojun Yuan, Fengxiang Jing, Shan Wu, Hongbo Zhou, Hongju Mao, Qinghui Jin, Jianlong Zhao, Hui Cong, Chunping Jia
Circulating tumor cells (CTCs) have attracted pretty much attention from scientists because of their important relationship with the process of metastasis. Here, we developed a size-based microfluidic chip containing triangular pillar array and filter channel array for detecting single CTCs and CTC clusters independent of tumor-specific markers. The cell populations in chip were characterized by immune-fluorescent staining combining an epithelial marker and a mesenchymal marker. We largely decreased the whole time of detection process to nearly 1...
December 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/28035949/the-effects-of-allicin-a-reactive-sulfur-species-from-garlic-on-a-selection-of-mammalian-cell-lines
#19
Martin C H Gruhlke, Carole Nicco, Frederic Batteux, Alan J Slusarenko
Garlic (Allium sativum L.) has been used as a spice and medicinal plant since ancient times. Garlic produces the thiol-reactive defence substance, allicin, upon wounding. The effects of allicin on human lung epithelium carcinoma (A549), mouse fibroblast (3T3), human umbilical vein endothelial cell (HUVEC), human colon carcinoma (HT29) and human breast cancer (MCF7) cell lines were tested. To estimate toxic effects of allicin, we used a standard MTT-test (methylthiazoltetrazolium) for cell viability and ³H-thymidine incorporation for cell proliferation...
December 26, 2016: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28035349/monoclonal-antibodies-targeting-non-small-cell-lung-cancer-stem-like-cells-by-multipotent-cancer-stem-cell-monoclonal-antibody-library
#20
Kaiyue Cao, Yunzhi Pan, Long Yu, Xiong Shu, Jing Yang, Linxin Sun, Lichao Sun, Zhihua Yang, Yuliang Ran
Cancer stem cells (CSCs) are a rare subset of cancer cells that play a significant role in cancer initiation, spreading, and recurrence. In this study, a subpopulation of lung cancer stem-like cells (LCSLCs) was identified from non-small cell lung carcinoma cell lines, SPCA-1 and A549, using serum-free suspension sphere-forming culture method. A monoclonal antibody library was constructed using immunized BLAB/c mice with the multipotent CSC cell line T3A-A3. Flow cytometry analysis showed that 33 mAbs targeted antigens can be enriched in sphere cells compared with the parental cells of SPCA-1 and A549 cell lines...
February 2017: International Journal of Oncology
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