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Fluorescence lung cancer

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https://www.readbyqxmd.com/read/28916277/circulating-tumor-cell-as-a-biomarker-for-evaluating-allogenic-nk-cell-immunotherapy-on-stage-iv-non-small-cell-lung-cancer
#1
Mao Lin, Shu-Zhen Liang, Jian Shi, Li-Zhi Niu, Ji-Bing Chen, Ming-Jie Zhang, Ke-Cheng Xu
In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-NK in patients with stage IV non- small cell lung cancer (NSCLC) as a reference for understanding the relevance of any changes to the efficacy of NK cells therapy. The patients were given one to three courses of immunotherapy. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 31 patients with stage IV NSCLC at 1day before and 7 and 30d after NK cells therapy using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR)...
September 12, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28915607/coal-tar-pitch-extract-could-induce-chromosomal-instability-of-human-bronchial-epithelial-cells-mediated-by-spindle-checkpoint-related-proteins
#2
Peng Zhang, Zhitao Li, Na Wang, Guangcai Duan, Wei Wang, Yanming Feng, Yong Zhao, Lixia Wang, Hansong Zhu, Qiao Zhang, Xiaozhuan Liu, Weidong Wu, Yongjun Wu, Wu Yao, Jing Wang, Yiming Wu, Feifei Feng
Coal tar pitch (CTP) is a byproduct of coal tar distillation. The workers working with coal tar or in aluminum smelters, potrooms and carbon plants have the opportunities of exposing to coal tar pitch volatiles. Coal tar pitches from which polycyclic aromatic hydrocarbons (PAHs) originate have been shown to exhibit lung carcinogenicity in humans. Chromosomal instability (CIN) is a mechanism in carcinogenesis, however, whether CIN is involved in coal tar pitch-induced lung cancer remains elusive. In this present study, human bronchial epithelial cells (BEAS-2B) were first exposed to coal tar pitch extracts (CTPE) to induce a malignant transformation model...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28914263/coexistent-genetic-alterations-involving-alk-ret-ros1-or-met-in-15-cases-of-lung-adenocarcinoma
#3
Zhenya Tang, Jianjun Zhang, Xinyan Lu, Wei Wang, Hui Chen, Melissa K Robinson, Joanne Cheng, Guilin Tang, L Jeffrey Medeiros
In lung cancer, targetable activating alterations in cancer genes, such as EGFR, ALK, RET, ROS1 and MET, are usually mutually exclusive. Rare lung cancer cases with coexistent alterations of EGFR and ALK or EGFR mutations with RET or ROS1 rearrangements have been reported. In this study, we report 15 patients (3 men and 12 women; 14 Caucasians and 1 African American) with ages ranging from 43 to 81 years (median 60 years) with lung adenocarcinoma in which coexistent alterations of two cancer-associated genes, including ALK, ROS1, or RET rearrangement or MET amplification were present...
September 15, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28912139/a-combination-rnai-chemotherapy-layer-by-layer-nanoparticle-for-systemic-targeting-of-kras-p53-with-cisplatin-to-treat-non-small-cell-lung-cancer
#4
Li Gu, Jason Z Deng, Sweta Roy, Paula T Hammond
PURPOSE: Mutation of the Kirsten ras sarcoma viral oncogene homolog (KRAS) and loss of p53 function are commonly seen in non-small cell lung cancer (NSCLC). Combining therapeutics targeting these tumor defensive pathways with cisplatin in a single nanoparticle platform are rarely developed in clinic. EXPERIMENTAL DESIGN: Cisplatin was encapsulated in liposomes which multiple polyelectrolyte layers including siKRAS and miR-34a were built on to generate multifunctional layer-by-layer nanoparticle...
September 14, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28906590/fgf19-genetic-amplification-as-a-potential-therapeutic-target-in-lung-squamous-cell-carcinomas
#5
Xiaochen Zhang, Mei Kong, Zhen Zhang, Suzhen Xu, Feifei Yan, Liyuan Wei, Jianying Zhou
BACKGROUND: Although FGF19 gene aberrations are associated with carcinogenesis and progression in human cancers, the roles of FGF19 genetic amplification and expression in Chinese patients with lung squamous cell carcinoma (LSCC) and FGF19 amplification as a potential therapeutic target for LSCC are not well understood. METHODS: Fluorescence in situ hybridization analysis and quantitative real-time-PCR was used to detect FGF19 genetic amplification and FGF19 messenger RNA expression in LSCC tumor and paired adjacent samples...
September 14, 2017: Thoracic Cancer
https://www.readbyqxmd.com/read/28901499/transfection-with-livin-and-survivin-shrna-inhibits-the-growth-and-proliferation-of-non%C3%A2-small-cell-lung-cancer-cells
#6
Qinmiao Huang, Yiming Zeng, Huihuang Lin, Huaping Zhang, Dongyong Yang
Lung cancer is reported to be a major public health issue worldwide and the overall prognosis of patients remains poor. The expression levels of Livin and Survivin, of the inhibitors of apoptosis (IAP) family, are associated with prognostic significance in the majority of solid tumors. Therefore, in the presents study, short hairpin (sh)RNA expression vectors inhibiting the Livin and Survivin genes were constructed to examine the effects of the transfection of Livin shRNA and/or Survivin shRNA on the biological functions of tumor cells...
September 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28892307/purification-of-polysaccharide-from-artificially-cultivated-anoectochilus-roxburghii-wall-lindl-by-high-speed-counter-current-chromatography-and-its-antitumor-activity
#7
Xiaoling Yu, Shouer Lin, Jinque Zhang, Liying Huang, Hong Yao, Shaoguang Li
To establish a systematic method for the extraction, purification, characterization and antitumor activity study of polysaccharide from artificially cultivated Anoectochilus roxburghii(wall.) Lindl. (AC-ARPS). High-speed counter current chromatography with two-phase aqueous systems was successfully applied to purify AC-ARPS after one-step separation. The purity of the AC-ARPS obtained by phenol/sulfuric acid method was 95.01%. The chemical structures of AC-ARPS were identified by a series analytical methods including high-performance liquid chromatography and liquid chromatography with mass spectrometry...
September 11, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28882160/intrinsic-fluorescence-of-the-clinically-approved-multikinase-inhibitor-nintedanib-reveals-lysosomal-sequestration-as-resistance-mechanism-in-fgfr-driven-lung-cancer
#8
Bernhard Englinger, Sebastian Kallus, Julia Senkiv, Daniela Heilos, Lisa Gabler, Sushilla van Schoonhoven, Alessio Terenzi, Patrick Moser, Christine Pirker, Gerald Timelthaler, Walter Jäger, Christian R Kowol, Petra Heffeter, Michael Grusch, Walter Berger
BACKGROUND: Studying the intracellular distribution of pharmacological agents, including anticancer compounds, is of central importance in biomedical research. It constitutes a prerequisite for a better understanding of the molecular mechanisms underlying drug action and resistance development. Hyperactivated fibroblast growth factor receptors (FGFRs) constitute a promising therapy target in several types of malignancies including lung cancer. The clinically approved small-molecule FGFR inhibitor nintedanib exerts strong cytotoxicity in FGFR-driven lung cancer cells...
September 7, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28881815/clinicopathological-characteristics-of-ros1-and-ret-rearranged-nsclc-in-caucasian-patients-data-from-a-cohort-of-713-non-squamous-nsclc-lacking-kras-egfr-her2-braf-pik3ca-alk-alterations
#9
Frédéric Dugay, Francisco Llamas-Gutierrez, Marjory Gournay, Sarah Medane, François Mazet, Dan Christian Chiforeanu, Emmanuelle Becker, Régine Lamy, Hervé Léna, Nathalie Rioux-Leclercq, Marc-Antoine Belaud-Rotureau, Florian Cabillic
Targeted therapies have substantially changed the management of non-small cell lung cancer (NSCLC) patients with driver oncogenes. Given the high frequency, EGFR and ALK aberrations were the first to be detected and paved the way for tyrosine kinase inhibitor (TKI) treatments. Other kinases such as ROS1 and more recently RET have emerged as promising targets, and ROS1 and RET TKIs are already available for precision medicine. We screened a large cohort of 713 Caucasian non-squamous NSCLC patients lacking EGFR/KRAS/BRAF/HER2/PI3KCA/ALK aberrations for ROS1 and RET rearrangements using fluorescence in situ hybridization to determine the frequency and clinicopathological characteristics of ROS1- and RET-positive patients...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28880029/fluorescence-labelling-of-extracellular-vesicles-using-a-novel-thiol-based-strategy-for-quantitative-analysis-of-cellular-delivery-and-intracellular-traffic
#10
H D Roberts-Dalton, A Cocks, J M Falcon-Perez, E J Sayers, J P Webber, P Watson, A Clayton, A T Jones
Extracellular vesicles, including exosomes, are naturally derived nanovesicles generated in and released by numerous cell types. As extracellular entities they have the capacity to interact with neighbouring cells and distant tissues and affect physiological processes as well as being implicated in numerous diseases including tumorigenesis and neurodegeneration. They are also under intense investigation as delivery vectors for biotherapeutics. The ways in which EVs interact with recipient cells to influence cell physiology and deliver a macromolecular payload are at the early stages of exploration...
September 7, 2017: Nanoscale
https://www.readbyqxmd.com/read/28875417/chlorogenic-acid-regulates-apoptosis-and-stem-cell-marker-related-gene-expression-in-a549-human-lung-cancer-cells
#11
Kazuo Yamagata, Yuri Izawa, Daiki Onodera, Motoki Tagami
Previous studies indicated that chlorogenic acid, a compound present in many fruits and vegetables, has anti-cancer activities. We report that chlorogenic acid regulates the expression of apoptosis-related genes and self-renewal-related stem cell markers in cancer cells. The lung cancer cell line A549 was cultured with or without chlorogenic acid. The presence of chlorogenic acid decreased cell proliferation as measured by MTT activity. Polymerase chain reaction (PCR) showed that treatment of cells with chlorogenic acid reduced the expression of BCL2 but increased that of both BAX and CASP3...
September 5, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28875330/liposomes-co-loaded-with-6-phosphofructo-2-kinase-fructose-2-6-biphosphatase-3-pfkfb3-shrna-plasmid-and-docetaxel-for-the-treatment-of-non-small-cell-lung-cancer
#12
Nusrat Chowdhury, Imran Vhora, Ketan Patel, Ravi Doddapaneni, Arindam Mondal, Mandip Singh
PURPOSE: Non-small cell lung cancer is the leading cause of cancer related deaths globally. Considering the side effects and diminishing chemosensitivity to chemotherapy, novel treatment approaches are sought. Hence, we aim to develop a liposomal co-delivery system of pDNA expressing shRNA against PFKFB3 (pshPFKFB3) and docetaxel (DTX). METHODS: Cationic DTX liposomes complexed with pshPFKFB3 (PSH-DL) were developed. In vitro cell line studies were performed to evaluate transfection, PFKFB3 mRNA silencing, cytotoxicity, pGP inhibition, and protein markers expression...
September 5, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28874076/multimodal-fluorescence-and-bioluminescence-imaging-reveals-transfection-potential-of-intratracheally-administered-polyplexes-for-breast-cancer-lung-metastases
#13
Antonia Geyer, Alexander Taschauer, Fatih Alioglu, Martina Anton, Julia Maier, Elisabeth Drothler, Manuela Simlinger, Sümeyye Yavuz, Haider Sami, Manfred Ogris
Local delivery of anticancer agents or gene therapeutics to lung tumors can circumvent side effects or accumulation in non-target organs, but accessibility via the alveolar side of blood-air barrier remains challenging. Polyplexes based on plasmid and linear polyethylenimine (LPEI) transfect healthy lung tissue when applied intravenously in the mouse, but direct delivery into the lungs results in low transfection of lung tissue. Nevertheless, LPEI could offer the potential to selectively transfect lung tumors, if accessible by the alveolar side...
September 6, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28869223/ros1-rearrangement-and-response-to-crizotinib-in-stage-iv-non-small-cell-lung-cancer
#14
Moushumi Suryavanshi, Manoj Kumar Panigrahi, Dushyant Kumar, Haristuti Verma, Mumtaz Saifi, Bharti Dabas, Ullas Batra, Dinesh Doval, Anurag Mehta
BACKGROUND: The frequency of ROS1 rearrangement in non-small cell lung cancers has been reported from 1.6% to 2.3%. MATERIALS AND METHODS: We examined 105 lung adenocarcinoma patients for ROS1 rearrangement which were negative for EGFR and anaplastic lymphoma kinase. Clinical characteristics of ROS1 rearranged patients and their responses to crizotinib therapy were studied. RESULTS: Of the 105 patients, three cases were positive for ROS1 rearrangement by fluorescence in situ hybridization analysis...
September 2017: Lung India: Official Organ of Indian Chest Society
https://www.readbyqxmd.com/read/28851360/phospholipid-phosphatase-4-promotes-proliferation-and-tumorigenesis-and-activates-ca-2-permeable-cationic-channel-in-lung-carcinoma-cells
#15
Xin Zhang, Lan Zhang, Bihua Lin, Xingxing Chai, Ronggang Li, Yuehua Liao, Xinghui Deng, Qiongru Liu, Wenli Yang, Yubo Cai, Wei Zhou, Zhichao Lin, Wenhai Huang, Meigong Zhong, Fangyong Lei, Jinhua Wu, Shuaishuai Yu, Xiaoping Li, Shangren Li, Yueyue Li, Jincheng Zeng, Wansheng Long, Dong Ren, Yanming Huang
BACKGROUND: Phospholipid phosphatase 4 (PPAPDC1A or PLPP4) has been demonstrated to be involved in the malignant process of many cancers. The purpose of this study was to investigate the clinical significance and biological roles of PLPP4 in lung carcinoma. METHODS: PLPP4 expression was examined in 8 paired lung carcinoma tissues by real-time PCR and in 265 lung carcinoma tissues by immunohistochemistry (IHC). Statistical analysis was performed to evaluate the clinical correlation between PLPP4 expression and clinicopathological features and survival in lung carcinoma patients...
August 29, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28846937/a-novel-imp1-inhibitor-btynb-targets-c-myc-and-inhibits-melanoma-and-ovarian-cancer-cell-proliferation
#16
Lily Mahapatra, Neal Andruska, Chengjian Mao, Jeremy Le, David J Shapiro
The oncofetal mRNA-binding protein, IMP1 or insulin-like growth factor-2 mRNA-binding protein 2 (IGF2BP1), binds to and stabilizes c-Myc, β-TrCP1, and other oncogenic mRNAs, leading to increased expression of the proteins encoded by its target mRNAs. IMP1 is frequently overexpressed in cancer and is strongly correlated with a poor prognosis and reduced survival in melanoma, ovarian, breast, colon, and lung cancer. While IMP1 is an attractive anticancer drug target, there are no small molecule inhibitors of IMP1...
August 25, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28842241/photodynamic-diagnosis-of-visceral-pleural-invasion-of-lung-cancer-with-a-combination-of-5-aminolevulinic-acid-and-autofluorescence-observation-systems
#17
Masahiro Kitada, Yoshinobu Ohsaki, Syunnsuke Yasuda, Masahiro Abe, Nana Takahashi, Satoshi Okazaki, Kei Ishibashi, Satoshi Hayashi
BACKGROUND: Visceral pleural invasion (PL) is a prognostic factor in lung cancer. In the lung, lymph flows along the pleura, in addition to the flow toward the pulmonary hilum just as the pulmonary arteries and veins run toward it. Even with the same tumor diameter, a PL1 or higher level of pleural invasion is indicative of a more advanced disease stage. Final diagnosis based on the PL level is made by pathological examination of excised specimens. However, if an intraoperative diagnosis can be established, proper selection of the surgical procedure can be made, and unnecessary surgeries for disseminated lesions can be avoided...
August 22, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28841191/structure-identification-and-in-vitro-anticancer-activity-of-lathyrol-3-phenylacetate-5-15-diacetate
#18
Jian-Ye Zhang, Wen-Jing Huang, Hong-Mei Sun, Yun Liu, Xiao-Qin Zhao, Si-Li Tang, Ming-Na Sun, Sheng Wang, Jia-Jun Li, Ling-Ling Zhang, Jun-Hua Zhou, Qian-Rong Pan, Hu-Biao Chen
Natural products from the genus Euphorbia show attention-attracting activities, such as anticancer activity. In this article, classical isolation and structure identification were used in a study on Caper Euphorbia Seed. Subsequently, MTT and wound healing assays, flow cytometry, western blotting, Hoechst 33258 staining and fluorescence microscopy examination were applied to investigate the anticancer activity of the obtained compounds. In a result, lathyrol-3-phenyl- acetate-5,15-diacetate (deoxy Euphorbia factor L1, DEFL1) was isolated from Caper Euphorbia Seed...
August 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28841044/a-multicomponent-microemulsion-using-rational-combination-strategy-improves-lung-cancer-treatment-through-synergistic-effects-and-deep-tumor-penetration
#19
Ding Qu, Mengfei Guo, Yue Qin, Lixiang Wang, Bing Zong, Yunyan Chen, Yan Chen
Previously, we have developed a multicomponent-based microemulsion composed of etoposide, coix seed oil, and ginsenoside Rh2 (ECG-MEs). In this study, our goal was to validate the feasibility of ECG-MEs in lung cancer treatment and explore the mechanism underling the enhanced antitumor efficacy. The optimal weight ratio of ginsenoside Rh2 (G-Rh2) in ECG-MEs was determined as 3% (wt%), that was capable of forming the microemulsion readily with small particle size and high drug encapsulation efficiency. In cellular studies, the intracellular fluorescence of human non-small cell lung cancer (A549) cells treated with fluorescein isothiocyanate-labeled ECG-MEs (FITC/ECG-MEs) was significantly higher than that of various controls, leading to the obviously synergistic anticancer activities in cytotoxicity and in vitro cell apoptosis induction...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28835082/-fluorescence-in-situ-hybridization-combined-with-cytomorphology-for-the-detection-of-lung-cancer-in-bronchial-brushing-specimens
#20
S S Lu, Q J Pan, J Cao, X Xu, H Zhao, D H Shen
Objective: To evaluate the diagnostic value of fluorescence in situ hybridization (FISH) combined with bronchial brushing cytology for detecting lung cancer. Methods: Centromeric enumeration probes (CEPs) for chromosomes 7, 8 and 17 were used in FISH assay. The combination of FISH and cytology was analyzed in 69 bronchial brushing specimens. Results: The positive rates of CEP7, CEP8 and CEP17 in malignant cases diagnosed by cytology were 50.0%, 80.8% and 65.4%, respectively. CEP8 probe showed significantly higher positive rate than CEP7 (P=0...
August 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
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