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https://www.readbyqxmd.com/read/27909571/affordable-art-in-kenya-the-only-hope-for-involuntary-childlessness
#1
S W Ndegwa
The reported overall subfertility rate in Kenia is 26.1% with 50% attributed to tubal factors and 15% due to male factors. This is probably an underestimation taking into consideration that due to the stigma and myths of the disease, many couples are seeking alternative care from religious sects, witchdoctors, herbalists. Because the costs associated with IVF in private centres are only affordable for the happy few, the only true hope for most Kenyans struggling with unintended childlessness lies in the introduction of affordable ART services...
June 27, 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/27868470/perinatal-outcomes-and-cost-effectivity-of-the-assisted-reproduction-pregnancies-with-advanced-age-a-retrospective-analysis
#2
Nafiye Yilmaz, Mustafa Kara, Bugra Coskun, Metin Kaba, Selçuk Erkilinc, Okan Yenicesu, Salim Erkaya
We demonstrated the IVF-ICSI results, perinatal outcomes and cost-effectivity of the patients with advanced age at a tertiary centre. A total of 456 patients categorised into two groups according to age: group 1 (n = 158) (≥39years) and group 2 (n = 298) (<39years) were analysed retrospectively. In addition, subgroup analysis was performed according to the 40 years cut-off. Clinical pregnancy rate was significantly different between the groups (p< .001). Preterm delivery (< 37 gestational week) and low birth weight (< 2500 g) were significantly higher in advanced aged women than youngsters (p< ...
November 20, 2016: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/27836979/a-mild-ovarian-stimulation-strategy-in-women-with-poor-ovarian-reserve-undergoing-ivf-a-multicenter-randomized-non-inferiority-trial
#3
M A Youssef, M van Wely, H Al-Inany, T Madani, N Jahangiri, S Khodabakhshi, M Alhalabi, M Akhondi, S Ansaripour, R Tokhmechy, L Zarandi, A Rizk, M El-Mohamedy, E Shaeer, M Khattab, M H Mochtar, F van der Veen
STUDY QUESTION: In subfertile women with poor ovarian reserve undergoing IVF does a mild ovarian stimulation strategy lead to comparable ongoing pregnancy rates in comparison to a conventional ovarian stimulation strategy? SUMMARY ANSWER: A mild ovarian stimulation strategy in women with poor ovarian reserve undergoing IVF leads to similar ongoing pregnancy rates as a conventional ovarian stimulation strategy. WHAT IS KNOWN ALREADY: Women diagnosed with poor ovarian reserve are treated with a conventional ovarian stimulation strategy consisting of high-dose gonadotropins and pituitary downregulation with a long mid-luteal start GnRH-agonist protocol...
November 11, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27831635/short-gonadotropin-releasing-hormone-agonist-versus-flexible-antagonist-versus-clomiphene-citrate-regimens-in-poor-responders-undergoing-in-vitro-fertilization-a-randomized-controlled-trial
#4
M Schimberni, F Ciardo, M Schimberni, A Giallonardo, V De Pratti, M Sbracia
OBJECTIVE: Poor responders represent a frustrating condition for couples undergoing IVF and clinicians, and their treatment remains disputed. To assess the efficacy and the most suitable protocol, we conducted a randomized controlled trial comparing three different protocols of ovarian stimulation in poor responder women: clomiphene citrate (CC) plus a high dose of gonadotropins and GnRH antagonist, flexible GnRH antagonist protocol and a short GnRH agonist protocol. PATIENTS AND METHODS: Between July 2014 and December 2015 we enrolled 250 poor responders in a previous IVF cycle at least 3 months before...
October 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27825533/no-need-for-luteal-phase-support-in-ivf-cycles-after-mild-stimulation-proof-of-concept-study
#5
Anna Pia Ferraretti, Paul Devroey, M Cristina Magli, Luca Gianaroli
This is a pilot study performed in a private IVF unit. The objective of the study was to investigate whether luteal support is required in IVF cycles after mild stimulation with clomiphene citrate and low FSH doses. The study included 15 patients with good prognosis (defined as ≤38 years old with normal ovarian reserve and normovulatory cycles, body mass index <29 kg/m(2), no previous cycles, no severe endometriosis, no history of recurrent miscarriage, no endocrine/autoimmune diseases and no surgical semen extraction from the partner) undergoing IVF with mild stimulation...
October 18, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27692676/sudden-death-and-cardiac-arrest-without-phenotype-the-utility-of-genetic-testing
#6
Yanushi D Wijeyeratne, Elijah R Behr
Approximately 4% of sudden cardiac deaths are unexplained [the sudden arrhythmic death syndrome (SADS)], and up to 6-10% of survivors of cardiac arrest do not have an identifiable cardiac abnormality after comprehensive clinical evaluation [idiopathic ventricular fibrillation (IVF)]. Genetic testing may be able to play a role in diagnostics and can be targeted to an underlying phenotype present in family members following clinical evaluation. Alternatively, post-mortem genetic testing (the "molecular autopsy") may diagnose the underlying cause if a clearly pathogenic rare variant is found...
August 31, 2016: Trends in Cardiovascular Medicine
https://www.readbyqxmd.com/read/27491771/pre-implantation-genetic-testing-in-art-who-will-benefit-and-what-is-the-evidence
#7
Alberto Vaiarelli, Danilo Cimadomo, Antonio Capalbo, Giovanna Orlando, Fabio Sapienza, Silvia Colamaria, Antonio Palagiano, Carlo Bulletti, Laura Rienzi, Filippo Maria Ubaldi
Pre-implantation genetic diagnosis for aneuploidy testing (PGD-A) is a tool to identify euploid embryos during IVF. The suggested populations of patients that can benefit from it are infertile women of advanced maternal age, with a history of recurrent miscarriages and/or IVF failures. However, a general consensus has not yet been reached.After the clinical failure of its first version based on cleavage stage biopsy and 9 chromosome-FISH analysis, PGD-A is currently performed by 24 chromosome screening techniques on trophectoderm (TE) biopsies...
October 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27486273/getting-older-women-pregnant-contemporary-thoughts
#8
Gautam N Allahbadia
For women of advanced age with abnormally increased FSH levels, standardized hormonal stimulation often represents a cost-intensive procedure with a low success rate. It is well established now that with mild ovarian stimulation, there is a greater percentage of good-quality eggs (although a smaller number) than with higher-dose conventional stimulation. Mild stimulation protocols reduce the mean number of days of stimulation, the total amount of gonadotropins used and the mean number of oocytes retrieved. The proportion of high-quality and euploid embryos seems to be higher compared with conventional stimulation protocols, and the pregnancy rate per embryo transfer is comparable...
October 2016: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/27121007/pipelle-for-pregnancy-pip-study-protocols-for-three-randomised-controlled-trials
#9
Sarah Lensen, Wellington Martins, Carolina Nastri, Lynn Sadler, Cindy Farquhar
BACKGROUND: The success rate of infertility treatments remains modest. Endometrial injury has been suggested as an intervention to increase the probability of pregnancy in women undergoing assisted reproductive technologies such as in vitro fertilisation (IVF). The majority of studies and systematic reviews have reported that endometrial injury improves the outcomes of IVF, intrauterine insemination and natural conception; however, the size and quality of the studies are poor. The low quality of the available evidence questions the presence of any real beneficial effect, and the applicability of the intervention in different populations remains unclear...
2016: Trials
https://www.readbyqxmd.com/read/26997248/factors-associated-with-the-use-of-elective-single-embryo-transfer-and-pregnancy-outcomes-in-the-united-states-2004-2012
#10
Aaron K Styer, Barbara Luke, Wendy Vitek, Mindy S Christianson, Valerie L Baker, Alicia Y Christy, Alex J Polotsky
OBJECTIVE: To evaluate factors associated with elective single-embryo transfer (eSET) utilization and its effect on assisted reproductive technology outcomes in the United States. DESIGN: Historical cohort. SETTING: Not applicable. PATIENT(S): Fresh IVF cycles of women aged 18-37 years using autologous oocytes with either one (SET) or two (double-embryo transfer [DET]) embryos transferred and reported to the Society for Assisted Reproductive Technology Clinic Outcome Reporting System between 2004 and 2012...
July 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/26984108/the-pellet-swim-up-is-the-best-technique-for-sperm-preparation-during-in-vitro-fertilization-procedures
#11
Aldo Volpes, Francesca Sammartano, Simona Rizzari, Salvatore Gullo, Angelo Marino, Adolfo Allegra
PURPOSE: The aim of this study was to investigate the most suitable sperm preparation technique to apply in order to obtain a spermatozoon population with minimal DNA damage during in vitro fertilization procedures. We compared four preparation techniques: direct swim-up (DSU), pellet swim-up (PSU), density gradient (DG), and density gradient followed by swim-up (DG-SU), evaluating the effects of each technique on the DNA damage rate, evaluated by DNA fragmentation index of the spermatozoa obtained...
June 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/26969653/cost-implications-for-subsequent-perinatal-outcomes-after-ivf-stratified-by-number-of-embryos-transferred-a-five-year-analysis-of-vermont-data
#12
Olivia J Carpinello, Peter R Casson, Chia-Ling Kuo, Renju S Raj, E Scott Sills, Christopher A Jones
BACKGROUND: In states in the USA without in vitro fertilzation coverage (IVF) insurance coverage, more embryos are transferred per cycle leading to higher risks of multi-fetal pregnancies and adverse pregnancy outcomes. OBJECTIVE: To determine frequency and cost of selected adverse perinatal complications based on number of embryos transferred during IVF, and calculate incremental cost per IVF live birth. METHODS: Medical records of patients who conceived with IVF (n = 116) and delivered at >20 weeks gestational age between 2007 and 2011 were evaluated...
June 2016: Applied Health Economics and Health Policy
https://www.readbyqxmd.com/read/26867335/-dual-cycle-and-dual-channel-regimen-in-the-treatment-of-severe-ovarian-hyperstimulation-syndrome-with-pregnancy
#13
La-mu Cidan, Li Chen, Dan Zhang
OBJECTIVE: To explore the therapeutic effects and advantages of dual channel and dual cycle regimen in the treatment of severe ovarian hyperstimulation syndrome (OHSS) with pregnancy. METHODS: This study enrolled 60 severe OHSS patients who were successfully pregnant with vitro fertilization-embryo transfer (IVF-ET). The patients were divided into conventional single cycle treatment group (Group A) and dual cycle and dual channel treatment group (Group B). Before the treatment and at the 3rd d and 9th d of treatment, all the patients received the measurements of body mass (BM), abdominal circumference (AC), maximum depth of ascites in ultrasonic (D), serum albumin (ALB), Na+ concentration, white blood cell count (WBC), hematocrit (HCT), drink intake (Intake) and urine volume...
November 2015: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/26692778/egg-recovery-completed-with-a-manually-created-negative-pressure-is-still-an-option-in-cases-of-emergency-or-low-cost-in-vitro-fertilization
#14
Theodoros Kalampokas, Abha Maheswari
This is a report of a case of an egg recovery procedure completed with manual suction instead of the automated negative pressure suction. A 35-year-old woman who was undergoing in vitro fertilization (IVF) treatment had to undergo oocyte recovery using manual suction through a syringe instead of the automated negative pressure suction systems because of the failure of both the initial and replacement systems. The treatment cycle ended in a positive pregnancy test and a clinical pregnancy with acceptable oocyte fertilization rates and no complications during procedure...
2015: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/26688556/effectiveness-of-semen-washing-to-prevent-human-immunodeficiency-virus-hiv-transmission-and-assist-pregnancy-in-hiv-discordant-couples-a-systematic-review-and-meta-analysis
#15
REVIEW
Maryam Zafer, Hacsi Horvath, Okeoma Mmeje, Sheryl van der Poel, Augusto E Semprini, George Rutherford, Joelle Brown
OBJECTIVE: To evaluate the effectiveness of semen washing in human immunodeficiency virus (HIV)-discordant couples in which the male partner is infected. DESIGN: Systematic review and meta-analysis. SETTING: Not applicable. PATIENT(S): Forty single-arm open-label studies among HIV-discordant couples that underwent intrauterine insemination (IUI) or in vitro fertilization (IVF) with or without intracytoplasmic sperm injection (ICSI) using washed semen...
March 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/26636621/complex-preimplantation-genetic-diagnosis-for-beta-thalassaemia-sideroblastic-anaemia-and-human-leukocyte-antigen-hla-typing
#16
Georgia Kakourou, Christina Vrettou, Antonis Kattamis, Aspasia Destouni, Myrto Poulou, Maria Moutafi, Georgia Kokkali, Konstantinos Pantos, Stephen Davies, Sophia Kitsiou-Tzeli, Emmanuel Kanavakis, Joanne Traeger-Synodinos
Preimplantation genetic diagnosis (PGD) to select histocompatible siblings to facilitate curative haematopoeitic stem-cell transplantation (HSCT) is now an acceptable option in the absence of an available human leukocyte antigen (HLA) compatible donor. We describe a case where the couple who requested HLA-PGD, were both carriers of two serious haematological diseases, beta-thalassaemia and sideroblastic anaemia. Their daughter, affected with sideroblastic anaemia, was programmed to have HSCT. A multiplex-fluorescent-touchdown-PCR protocol was optimized for the simultaneous amplification of: the two HBB-gene mutated regions (c...
2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/26558801/gonadotrophin-releasing-hormone-agonist-protocols-for-pituitary-suppression-in-assisted-reproduction
#17
REVIEW
Charalampos S Siristatidis, Ahmed Gibreel, George Basios, Abha Maheshwari, Siladitya Bhattacharya
BACKGROUND: Gonadotrophin-releasing hormone agonists (GnRHa) are commonly used in assisted reproduction technology (ART) cycles to prevent a luteinising hormone surge during controlled ovarian hyperstimulation (COH) prior to planned oocyte retrieval, thus optimising the chances of live birth. OBJECTIVES: To evaluate the effectiveness of the different GnRHa protocols as adjuncts to COH in women undergoing ART cycles. SEARCH METHODS: We searched the following databases from inception to April 2015: the Cochrane Menstrual Disorders and Subfertility Group Specialised Register, the Cochrane Central Register of Controlled Trials (CENTRAL) in the Cochrane Library (2015, Issue 3), MEDLINE, EMBASE, CINAHL, PsycINFO, and registries of ongoing trials...
November 9, 2015: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/26524503/transgenic-expression-of-green-fluorescent-protein-in-caprine-embryos-produced-through-electroporation-aided-sperm-mediated-gene-transfer
#18
R Kumar Pramod, Rakesh Kumar, Abhijit Mitra
Current methods of transgenic animal production are afflicted by low efficiency and high cost. Recently, the electroporation aided sperm-mediated gene transfer (SMGT) emerges as a promising alternative with variable success rate. Among the domestic animal species, the electroporation-aided SMGT is less investigated in goats, except a few reports in which attempts have been made using the auto-uptake method of SMGT. In this study, we report an optimized electroporation condition for SMGT of caprine sperm cells...
January 15, 2016: Gene
https://www.readbyqxmd.com/read/26209535/daily-low-dose-hcg-stimulation-during-the-luteal-phase-combined-with-gnrha-triggered-ivf-cycles-without-exogenous-progesterone-a-proof-of-concept-trial
#19
RANDOMIZED CONTROLLED TRIAL
Claus Yding Andersen, Helle Olesen Elbaek, Birgit Alsbjerg, Rita Jakubcionyte Laursen, Betina Boel Povlsen, Lise Thomsen, Peter Humaidan
STUDY QUESTION: Can the luteal phase support be improved in terms of efficacy, hormonal profiles and convenience as compared with today's standard care? SUMMARY ANSWER: Daily low-dose rhCG supplementation in GnRHa triggered IVF cycles can replace the traditional used luteal phase support with exogenous progesterone. WHAT IS KNOWN ALREADY: A bolus of hCG for final maturation of follicles in connection with COS may induce the risk of OHSS and the luteal phase progesterone levels rise very abruptly in the early luteal phase...
October 2015: Human Reproduction
https://www.readbyqxmd.com/read/26193891/low-dose-growth-hormone-supplementation-increases-clinical-pregnancy-rate-in-poor-responders-undergoing-in-vitro-fertilisation
#20
Karinna Lattes, Mario Brassesco, Manuel Gomez, Miguel A Checa
Poor ovarian response (POR) often means low success rates after in vitro fertilisation (IVF). We aim to study the impact of a low-dose growth hormone (GH) supplementation in pregnancy rates in poor responders in a prospective, self-controlled study of 64 poor responders to previous IVF cycles, who failed to achieve pregnancy and were supplemented with low-doses of GH in a subsequent cycle using the same gonadotropin dose and protocol. Our primary endpoint was the clinical pregnancy rate (CPR), considering secondary endpoints, the number of retrieved oocytes, embryos, embryo quality and the proportion of cycles with embryo transfer...
July 2015: Gynecological Endocrinology
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