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multiphoton imaging

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https://www.readbyqxmd.com/read/28063434/integral-steric-asymmetry-in-the-inelastic-scattering-of-no-x-2-%C3%AE
#1
M Brouard, S D S Gordon, A Hackett Boyle, C G Heid, B Nichols, V Walpole, F J Aoiz, S Stolte
The integral steric asymmetry for the inelastic scattering of NO(X) by a variety of collision partners was recorded using a crossed molecular beam apparatus. The initial state of the NO(X, v = 0, j = 1/2, Ω=1/2, ϵ=-1,f) molecule was selected using a hexapole electric field, before the NO bond axis was oriented in a static electric field, allowing probing of the scattering of the collision partner at either the N- or O-end of the molecule. Scattered NO molecules were state selectively probed using (1 + 1') resonantly enhanced multiphoton ionisation, coupled with velocity-map ion imaging...
January 7, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28063036/visualization-of-the-t-cell-response-in-contact-hypersensitivity
#2
Gyohei Egawa, Kenji Kabashima
Contact hypersensitivity (CHS) is the most basic murine model for type IV hypersensitivity. This dermatitis model is mediated by hapten-sensitized, skin-infiltrating T cells. Recent intravital imaging studies have demonstrated the dynamic migratory property of skin-infiltrating T cells and the orchestrated interaction of T cells with dermal dendritic cells. Multiphoton microscopy enables the direct, three-dimensional, and minimally invasive imaging of in vivo skin samples with high spatiotemporal resolution...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28060277/inducing-ischemia-reperfusion-injury-in-the-mouse-ear-skin-for-intravital-multiphoton-imaging-of-immune-responses
#3
Chi Ching Goh, Jackson LiangYao Li, David Becker, Wolfgang Weninger, Veronique Angeli, Lai Guan Ng
Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent re-oxygenation of the tissues (reperfusion). In the skin, ischemia-reperfusion (IR) is the main contributing factor to the pathophysiology of pressure ulcers. While the cascade of events leading up to the inflammatory response has been well studied, the spatial and temporal responses of the different subsets of immune cells to an IR injury are not well understood...
December 22, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28059135/labeling-and-in-vivo-visualization-of-transplanted-adipose-tissue-derived-stem-cells-with-safe-cadmium-free-aqueous-zns-coating-of-zns-agins2-nanoparticles
#4
Yusuke Ogihara, Hiroshi Yukawa, Tatsuya Kameyama, Hiroyasu Nishi, Daisuke Onoshima, Tetsuya Ishikawa, Tsukasa Torimoto, Yoshinobu Baba
The facile synthesis of ZnS-AgInS2 (ZAIS) as cadmium-free QDs and their application, mainly in solar cells, has been reported by our groups. In the present study, we investigated the safety and the usefulness for labeling and in vivo imaging of a newly synthesized aqueous ZnS-coated ZAIS (ZnS-ZAIS) carboxylated nanoparticles (ZZC) to stem cells. ZZC shows the strong fluorescence in aqueous solutions such as PBS and cell culture medium, and a complex of ZZC and octa-arginine (R8) peptides (R8-ZZC) can achieve the highly efficient labeling of adipose tissue-derived stem cells (ASCs)...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043893/a-novel-microstructural-interpretation-for-the-biomechanics-of-mouse-skin-derived-from-multiscale-characterization
#5
Barbara Lynch, Stéphane Bancelin, Christelle Bonod-Bidaud, Jean-Baptiste Gueusquin, Florence Ruggiero, Marie-Claire Schanne-Klein, Jean-Marc Allain
: Skin is a complex, multi-layered organ, with important functions in the protection of the body. The dermis provides structural support to the epidermal barrier, and thus has attracted a large number of mechanical studies. As the dermis is made of a mixture of stiff fibres embedded in a soft non-fibrillar matrix, it is classically considered that its mechanical response is based on an initial alignment of the fibres, followed by the stretching of the aligned fibres. Using a recently developed setup combining multiphoton microscopy with mechanical assay, we imaged the fibres network evolution during dermis stretching...
December 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/28036196/superresolving-imaging-of-arbitrary-one-dimensional-arrays-of-thermal-light-sources-using-multiphoton-interference
#6
Anton Classen, Felix Waldmann, Sebastian Giebel, Raimund Schneider, Daniel Bhatti, Thomas Mehringer, Joachim von Zanthier
We propose to use multiphoton interferences of photons emitted from statistically independent thermal light sources in combination with linear optical detection techniques to reconstruct, i.e., image, arbitrary source geometries in one dimension with subclassical resolution. The scheme is an extension of earlier work [S. Oppel et al., Phys. Rev. Lett. 109, 233603 (2012)], where N regularly spaced sources in one dimension were imaged by use of the Nth-order intensity correlation function. Here, we generalize the scheme to reconstruct any number of independent thermal light sources at arbitrary separations in one dimension, exploiting intensity correlation functions of order m≥3...
December 16, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27998729/in%C3%A2-vivo-imaging-of-inflamed-glomeruli-reveals-dynamics-of-neutrophil-extracellular-trap-formation-in-glomerular-capillaries
#7
Clare L V Westhorpe, James E Bayard, Kim M O'Sullivan, Pam Hall, Qiang Cheng, A Richard Kitching, Michael J Hickey
Neutrophil extracellular traps (NETs) have been documented in glomeruli of patients with glomerulonephritis. However, the dynamics of NET formation in the glomerulus and their functional contribution to acute glomerular injury are poorly understood. Herein, we used in vivo multiphoton microscopy to investigate NET formation in the acutely inflamed glomerulus. Glomerular inflammation was induced using an antibody against the glomerular basement membrane. After induction of glomerular inflammation, multiphoton microscopy revealed that approximately 20% of glomeruli contained structures composed of extracellular DNA within the capillaries...
December 17, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27992081/non-invasive-metabolic-imaging-of-melanoma-progression
#8
Michael N Pastore, Hauke Studier, Claudine S Bonder, Michael S Roberts
Skin cancer is associated with abnormal cellular metabolism which if identified early introduces the possibility of intervention to prevent its progress to a deadly metastatic stage. This study combines multiphoton microscopy with fluorescence lifetime imaging (FLIM) using an syngeneic melanoma mouse model, to detect changes in metabolic state of single epidermal cells as a metabolic marker to monitor the progress of tumor growth. This method utilizes imaging of the ratio of the amounts of the free and protein-bound forms of the intracellular autofluorescent metabolic co-enzyme nicotinamide adenine dinucleotide (NADH)...
December 19, 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/27991861/astrocytic-calcium-release-mediates-peri-infarct-depolarizations-in-a-rodent-stroke-model
#9
Cordula Rakers, Gabor C Petzold
Stroke is one of the most common diseases and a leading cause of death and disability. Cessation of cerebral blood flow (CBF) leads to cell death in the infarct core, but tissue surrounding the core has the potential to recover if local reductions in CBF are restored. In these areas, detrimental peri-infarct depolarizations (PIDs) contribute to secondary infarct growth and negatively affect stroke outcome. However, the cellular pathways underlying PIDs have remained unclear. Here, we have used in vivo multiphoton microscopy, laser speckle imaging of CBF, and electrophysiological recordings in a mouse model of focal ischemia to demonstrate that PIDs are associated with a strong increase of intracellular calcium in astrocytes and neurons...
December 19, 2016: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27983804/quantitative-chemical-imaging-of-non-planar-microfluidics
#10
Matthew K Gelber, Matthew R Kole, Namjung Kim, Narayana R Aluru, Rohit Bhargava
Confocal and multiphoton optical imaging techniques have been powerful tools for evaluating the performance of and monitoring experiments within microfluidic devices, but this application suffers from two pitfalls. The first is that obtaining the necessary imaging contrast often requires the introduction of an optical label which can potentially change the behavior of the system. The emerging analytical technique stimulated Raman scattering (SRS) microscopy promises a solution, as it can rapidly measure 3D concentration maps based on vibrational spectra, label-free; however, when using any optical imaging technique, including SRS, there is an additional problem of optical aberration due to refractive index mismatch between the fluid and the device walls...
December 16, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27956241/real-time-and-quantitative-fluorescent-live-cell-imaging-with-quadruplex-specific-red-edge-probe-g4-rep
#11
Sunny Y Yang, Souheila Amor, Aurélien Laguerre, Judy M Y Wong, David Monchaud
The development of quadruplex-directed molecular diagnostic and therapy rely on mechanistic insights gained at both cellular and tissue levels by fluorescence imaging. This technique is based on fluorescent reporters that label cellular DNA and RNA quadruplexes to spatiotemporally address their complex cell biology. The photophysical characteristics of quadruplex probes usually dictate the modality of cell imaging by governing the selection of the light source (lamp, LED, laser), the optical light filters and the detection modality...
December 10, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27941379/can-we-improve-transurethral-resection-of-the-bladder-tumour-for-nonmuscle-invasive-bladder-cancer
#12
Esmee Iml Liem, Theo M de Reijke
PURPOSE OF REVIEW: The recurrence rate in patients with nonmuscle invasive bladder cancer is high, and the quality of transurethral resection of the bladder (TURB) tumour influences recurrence risk. We review new methods that aim to improve the effectiveness of TURB, and highlight studies of the past year. RECENT FINDINGS: Several approaches have been explored: bipolar resection is well tolerated and efficient; however, surgical outcomes are not clearly superior to monopolar resection; en-bloc resection seems feasible and well tolerated, and has the potential to improve the quality of tissue for histopathology; enhancement techniques such as photodynamic diagnosis, narrow band imaging, and Image1 S improve tumour detection, whereas photodynamic diagnosis reduces recurrence rates; high-resolution imaging modality such as confocal laser endomicroscopy may provide histopathologic information...
December 9, 2016: Current Opinion in Urology
https://www.readbyqxmd.com/read/27905111/depth-enhancement-of-3d-microscopic-living-cell-image-using-incoherent-fluorescent-digital-holography
#13
L T Bang, H Y Wu, Y Zhao, E G Kim, N Kim
Multilayer images of living cells are typically obtained using confocal or multiphoton microscopy. However, limitations on the distance between consecutive scan layers hinder high-resolution three-dimensional reconstruction, and scattering strongly degrades images of living cell components. Consequently, when overlapping information from different layers is focused on a specific point in the camera, this causes uncertainty in the depiction of the cell components. We propose a method that combines the Fresnel incoherent correlation holography and a depth-of-focus reduction algorithm to enhance the depth information of three-dimensional cell images...
December 1, 2016: Journal of Microscopy
https://www.readbyqxmd.com/read/27895980/rapid-mesoscale-multiphoton-microscopy-of-human-skin
#14
Mihaela Balu, Hideharu Mikami, Jue Hou, Eric O Potma, Bruce J Tromberg
We present a multiphoton microscope designed for mesoscale imaging of human skin. The system is based on two-photon excited fluorescence and second-harmonic generation, and images areas of ~0.8x0.8 mm(2) at speeds of 0.8 fps (800x800 pixels; 12 frame averages) for high signal-to-noise ratio, with lateral and axial resolutions of 0.5µm and 3.3µm, respectively. The main novelty of this instrument is the design of the scan head, which includes a fast galvanometric scanner, optimized relay optics, a beam expander and high NA objective lens...
November 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27893712/blood-vessel-endothelium-directed-tumor-cell-streaming-in-breast-tumors-requires-the-hgf-c-met-signaling-pathway
#15
E Leung, A Xue, Y Wang, P Rougerie, V P Sharma, R Eddy, D Cox, J Condeelis
During metastasis to distant sites, tumor cells migrate to blood vessels. In vivo, breast tumor cells utilize a specialized mode of migration known as streaming, where a linear assembly of tumor cells migrate directionally towards blood vessels on fibronectin-collagen I-containing extracellular matrix (ECM) fibers in response to chemotactic signals. We have successfully reconstructed tumor cell streaming in vitro by co-plating tumors cells, macrophages and endothelial cells on 2.5 μm thick ECM-coated micro-patterned substrates...
November 28, 2016: Oncogene
https://www.readbyqxmd.com/read/27888509/functional-properties-of-granule-cells-with-hilar-basal-dendrites-in-the-epileptic-dentate-gyrus
#16
Tony Kelly, Heinz Beck
OBJECTIVE: The maturation of adult-born granule cells and their functional integration into the network is thought to play a key role in the proper functioning of the dentate gyrus. In temporal lobe epilepsy, adult-born granule cells in the dentate gyrus develop abnormally and possess a hilar basal dendrite (HBD). Although morphological studies have shown that these HBDs have synapses, little is known about the functional properties of these HBDs or the intrinsic and network properties of the granule cells that possess these aberrant dendrites...
January 2017: Epilepsia
https://www.readbyqxmd.com/read/27886282/dynamics-and-yields-for-chbrcl2-photodissociation-from-215-265-nm
#17
Wyatt G Merrill, F Fleming Crim, Amanda S Case
We investigate the Ã-band photodissociation of CHBrCl2 at 215, 225, 235, 245, 255, and 265 nm. Following C-Br bond cleavage, resonance enhanced multiphoton ionization and time of flight mass spectrometry provide selective detection of the two product channels, from which we quantify the relative quantum yield of Br/Br* production. Velocity-map imaging of the photofragments allows us to determine the energy partitioning as a function of the photolysis energy for different exit channels. The anisotropy present in the imaging data suggests that absorption to the (3)Q0(+)(A') state is important throughout the entire region we study, though competition with other excited states is evident...
December 7, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27878858/non-invasive-tools-for-the-diagnosis-of-cutaneous-melanoma
#18
REVIEW
C Fink, H A Haenssle
BACKGROUND: While the excisional biopsy and histological examination of suspicious lesions remains the current gold standard for diagnosing cutaneous melanoma (CM), there is a demand for more objective and non-invasive examination methods that may support clinicians in their decision when to biopsy or not. METHODS: This review is based on publications and guidelines retrieved by a selective search in PubMed and MEDLINE and focused on non-invasive diagnostic strategies for detecting melanoma...
November 22, 2016: Skin Research and Technology
https://www.readbyqxmd.com/read/27878400/multiphoton-microscopy-observations-of-3d-elastin-and-collagen-fiber-microstructure-changes-during-pressurization-in-aortic-media
#19
Shukei Sugita, Takeo Matsumoto
Elastin and collagen fibers play important roles in the mechanical properties of aortic media. Because knowledge of local fiber structures is required for detailed analysis of blood vessel wall mechanics, we investigated 3D microstructures of elastin and collagen fibers in thoracic aortas and monitored changes during pressurization. Using multiphoton microscopy, autofluorescence images from elastin and second harmonic generation signals from collagen were acquired in media from rabbit thoracic aortas that were stretched biaxially to restore physiological dimensions...
November 22, 2016: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/27858361/high-resolution-intravital-microscopy-of-tumor-angiogenesis
#20
Ann L B Seynhaeve, Timo L M Ten Hagen
Real-time evaluation of vascular effects in an animal skinfold window model by intravital microscopy (IVM) provides a powerful tool to improve insight into vascular development and vascular therapy. The potential of IVM to examine processes in tissues (e.g., tumors, inflammatory sites), in a noninvasive way, enables determination of the kinetics of processes under study at any given time point. The introduction of sensitive digital cameras, confocal and multiphoton microscopy, and powerful imaging software greatly improved the quality of the images acquired...
2016: Methods in Molecular Biology
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