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multiphoton imaging

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https://www.readbyqxmd.com/read/27905111/depth-enhancement-of-3d-microscopic-living-cell-image-using-incoherent-fluorescent-digital-holography
#1
L T Bang, H Y Wu, Y Zhao, E G Kim, N Kim
Multilayer images of living cells are typically obtained using confocal or multiphoton microscopy. However, limitations on the distance between consecutive scan layers hinder high-resolution three-dimensional reconstruction, and scattering strongly degrades images of living cell components. Consequently, when overlapping information from different layers is focused on a specific point in the camera, this causes uncertainty in the depiction of the cell components. We propose a method that combines the Fresnel incoherent correlation holography and a depth-of-focus reduction algorithm to enhance the depth information of three-dimensional cell images...
December 1, 2016: Journal of Microscopy
https://www.readbyqxmd.com/read/27895980/rapid-mesoscale-multiphoton-microscopy-of-human-skin
#2
Mihaela Balu, Hideharu Mikami, Jue Hou, Eric O Potma, Bruce J Tromberg
We present a multiphoton microscope designed for mesoscale imaging of human skin. The system is based on two-photon excited fluorescence and second-harmonic generation, and images areas of ~0.8x0.8 mm(2) at speeds of 0.8 fps (800x800 pixels; 12 frame averages) for high signal-to-noise ratio, with lateral and axial resolutions of 0.5µm and 3.3µm, respectively. The main novelty of this instrument is the design of the scan head, which includes a fast galvanometric scanner, optimized relay optics, a beam expander and high NA objective lens...
November 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27893712/blood-vessel-endothelium-directed-tumor-cell-streaming-in-breast-tumors-requires-the-hgf-c-met-signaling-pathway
#3
E Leung, A Xue, Y Wang, P Rougerie, V P Sharma, R Eddy, D Cox, J Condeelis
During metastasis to distant sites, tumor cells migrate to blood vessels. In vivo, breast tumor cells utilize a specialized mode of migration known as streaming, where a linear assembly of tumor cells migrate directionally towards blood vessels on fibronectin-collagen I-containing extracellular matrix (ECM) fibers in response to chemotactic signals. We have successfully reconstructed tumor cell streaming in vitro by co-plating tumors cells, macrophages and endothelial cells on 2.5 μm thick ECM-coated micro-patterned substrates...
November 28, 2016: Oncogene
https://www.readbyqxmd.com/read/27888509/functional-properties-of-granule-cells-with-hilar-basal-dendrites-in-the-epileptic-dentate-gyrus
#4
Tony Kelly, Heinz Beck
OBJECTIVE: The maturation of adult-born granule cells and their functional integration into the network is thought to play a key role in the proper functioning of the dentate gyrus. In temporal lobe epilepsy, adult-born granule cells in the dentate gyrus develop abnormally and possess a hilar basal dendrite (HBD). Although morphological studies have shown that these HBDs have synapses, little is known about the functional properties of these HBDs or the intrinsic and network properties of the granule cells that possess these aberrant dendrites...
November 26, 2016: Epilepsia
https://www.readbyqxmd.com/read/27886282/dynamics-and-yields-for-chbrcl2-photodissociation-from-215-265-nm
#5
Wyatt G Merrill, F Fleming Crim, Amanda S Case
We investigate the Ã-band photodissociation of CHBrCl2 at 215, 225, 235, 245, 255, and 265 nm. Following C-Br bond cleavage, resonance enhanced multiphoton ionization and time of flight mass spectrometry provide selective detection of the two product channels, from which we quantify the relative quantum yield of Br/Br* production. Velocity-map imaging of the photofragments allows us to determine the energy partitioning as a function of the photolysis energy for different exit channels. The anisotropy present in the imaging data suggests that absorption to the (3)Q0(+)(A') state is important throughout the entire region we study, though competition with other excited states is evident...
November 25, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27878858/non-invasive-tools-for-the-diagnosis-of-cutaneous-melanoma
#6
REVIEW
C Fink, H A Haenssle
BACKGROUND: While the excisional biopsy and histological examination of suspicious lesions remains the current gold standard for diagnosing cutaneous melanoma (CM), there is a demand for more objective and non-invasive examination methods that may support clinicians in their decision when to biopsy or not. METHODS: This review is based on publications and guidelines retrieved by a selective search in PubMed and MEDLINE and focused on non-invasive diagnostic strategies for detecting melanoma...
November 22, 2016: Skin Research and Technology
https://www.readbyqxmd.com/read/27878400/multiphoton-microscopy-observations-of-3d-elastin-and-collagen-fiber-microstructure-changes-during-pressurization-in-aortic-media
#7
Shukei Sugita, Takeo Matsumoto
Elastin and collagen fibers play important roles in the mechanical properties of aortic media. Because knowledge of local fiber structures is required for detailed analysis of blood vessel wall mechanics, we investigated 3D microstructures of elastin and collagen fibers in thoracic aortas and monitored changes during pressurization. Using multiphoton microscopy, autofluorescence images from elastin and second harmonic generation signals from collagen were acquired in media from rabbit thoracic aortas that were stretched biaxially to restore physiological dimensions...
November 22, 2016: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/27858361/high-resolution-intravital-microscopy-of-tumor-angiogenesis
#8
Ann L B Seynhaeve, Timo L M Ten Hagen
Real-time evaluation of vascular effects in an animal skinfold window model by intravital microscopy (IVM) provides a powerful tool to improve insight into vascular development and vascular therapy. The potential of IVM to examine processes in tissues (e.g., tumors, inflammatory sites), in a noninvasive way, enables determination of the kinetics of processes under study at any given time point. The introduction of sensitive digital cameras, confocal and multiphoton microscopy, and powerful imaging software greatly improved the quality of the images acquired...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27847119/preclinical-advances-with-multiphoton-microscopy-in-live-imaging-of-skin-cancers
#9
REVIEW
Thomas Yang Sun, Ann M Haberman, Valentina Greco
Conventional, static analyses have historically been the bedrock and tool of choice for the study of skin cancers. Over the past several years, in vivo imaging of tumors using multiphoton microscopy has emerged as a powerful preclinical tool for revealing detailed cellular behaviors from the earliest moments of tumor development to the final steps of metastasis. Multiphoton microscopy allows for deep tissue penetration with relatively minor phototoxicity, rendering it an effective tool for the long-term observation of tumor evolution...
November 12, 2016: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/27816622/electrospun-poly-l-lactide-scaffold-for-the-controlled-and-targeted-delivery-of-a-synthetically-obtained-diclofenac-prodrug-to-treat-actinic-keratosis
#10
Germano Piccirillo, Brigida Bochicchio, Antonietta Pepe, Katja Schenke-Layland, Svenja Hinderer
: Actinic Keratosis' (AKs) are small skin lesions that are related to a prolonged sun-damage, which can develop into invasive squamous cell carcinoma (SCC) when left untreated. Effective, specific and well tolerable therapies to cure AKs are still of great interest. Diclofenac (DCF) is the current gold standard for the local treatment of AKs in terms of costs, effectiveness, side effects and tolerability. In this work, an electrospun polylactic acid (PLA) scaffold loaded with a synthetic DCF prodrug was developed and characterized...
November 2, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27810931/immunotherapy-with-aducanumab-restores-calcium-homeostasis-in-tg2576-mice
#11
Ksenia V Kastanenka, Thierry Bussiere, Naomi Shakerdge, Fang Qian, Paul H Weinreb, Ken Rhodes, Brian J Bacskai
: Calcium homeostasis plays a major role in maintaining neuronal function under physiological conditions. Amyloid-β (Aβ) initiates pathological processes that include disruption in intracellular calcium levels, and therefore amelioration of the calcium alteration could serve as an indirect functional indicator of treatment efficacy. Thus, calcium dynamics was used as a measure of functional outcome. We evaluated the effects of the anti-Aβ antibody aducanumab, on calcium homeostasis and plaque clearance in aged Tg2576 mice with in vivo multiphoton imaging...
November 3, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27805057/fluselenamyl-a-novel-benzoselenazole-derivative-for-pet-detection-of-amyloid-plaques-a%C3%AE-in-alzheimer-s-disease
#12
G S M Sundaram, Dhruva D Dhavale, Julie L Prior, Ping Yan, John Cirrito, Nigam P Rath, Richard Laforest, Nigel J Cairns, Jin-Moo Lee, Paul T Kotzbauer, Vijay Sharma
Fluselenamyl (5), a novel planar benzoselenazole shows traits desirable of enabling noninvasive imaging of Aβ pathophysiology in vivo; labeling of both diffuse (an earlier manifestation of neuritic plaques) and fibrillar plaques in Alzheimer's disease (AD) brain sections, and remarkable specificity for mapping Aβ compared with biomarker proteins of other neurodegenerative diseases. Employing AD homogenates, [(18)F]-9, a PET tracer demonstrates superior (2-10 fold higher) binding affinity than approved FDA tracers, while also indicating binding to high affinity site on Aβ plaques...
November 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27793045/serpin-e2-promotes-breast-cancer-metastasis-by-remodeling-the-tumor-matrix-and-polarizing-tumor-associated-macrophages
#13
Tatiana Smirnova, Laura Bonapace, Gwen MacDonald, Shunya Kondo, Jeffrey Wyckoff, Hilmar Ebersbach, Bérengère Fayard, Arno Doelemeyer, Marie-May Coissieux, Marinus R Heideman, Mohamed Bentires-Alj, Nancy E Hynes
The extracellular serine protease inhibitor serpinE2 is overexpressed in breast cancer and has been shown to foster metastatic spread. Here, we investigated the hypothesis that serpinE2 creates tumor-promoting conditions in the tumor microenvironment (TME) by affecting extracellular matrix remodeling. Using two different breast cancer models, we show that blocking serpinE2, either by knock-down (KD) in tumor cells or in response to a serpinE2 binding antibody, decreases metastatic dissemination from primary tumors to the lungs...
October 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27790387/direct-visualization-of-the-phenotype-of-hypoxic-tumor-cells-at-single-cell-resolution-in-vivo-using-a-new-hypoxia-probe
#14
Yarong Wang, Haoxuan Wang, Jiufeng Li, David Entenberg, Alice Xue, Weigang Wang, John Condeelis
Tumor hypoxia is linked to tumor progression, metastasis, and therapy resistance. However, the underlying mechanisms behind this linkage are not fully understood. Here we present a novel fluorescent mCherry hypoxia-responsive marker that can be used in real time imaging to specifically and sensitively identify hypoxic cells in vivo at single cell resolution. Tumors derived from triple negative tumor cells expressing the hypoxia marker reveal that the hypoxic tumor cells congregate near flowing blood vessels...
2016: IntraVital
https://www.readbyqxmd.com/read/27786461/painting-with-rainbows-patterning-light-in-space-time-and-wavelength-for-multiphoton-optogenetic-sensing-and-control
#15
Daan Brinks, Yoav Adam, Simon Kheifets, Adam E Cohen
Photons are a fascinating reagent, flowing and reacting quite differently compared to more massive and less ephemeral particles of matter. The optogenetic palette comprises an ever growing set of light-responsive proteins, which open the possibility of using light to perturb and to measure biological processes with great precision in space and time. Yet there are limits on what light can achieve. Diffraction limits the smallest features, and scattering in tissue limits the largest. Photobleaching, diffusion of photogenerated products, and optical crosstalk between overlapping absorption spectra further muddy the optogenetic picture, particularly when one wants to use multiple optogenetic tools simultaneously...
October 27, 2016: Accounts of Chemical Research
https://www.readbyqxmd.com/read/27782625/wavelength-dependent-photoelectron-circular-dichroism-of-limonene-studied-by-femtosecond-multiphoton-laser-ionization-and-electron-ion-coincidence-imaging
#16
Mohammad M Rafiee Fanood, Maurice H M Janssen, Ivan Powis
Enantiomers of the monoterpene limonene have been investigated by (2 + 1) resonance enhanced multiphoton ionization and photoelectron circular dichroism employing tuneable, circularly polarized femtosecond laser pulses. Electron imaging detection provides 3D momentum measurement while electron-ion coincidence detection can be used to mass-tag individual electrons. Additional filtering, by accepting only parent ion tagged electrons, can be then used to provide discrimination against higher energy dissociative ionization mechanisms where more than three photons are absorbed to better delineate the two photon resonant, one photon ionization pathway...
September 28, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27779396/near-infrared-photoacoustic-imaging-probe-responsive-to-calcium
#17
Anurag Mishra, Yuanyuan Jiang, Sheryl Roberts, Vasilis Ntziachristos, Gil Gregor Westmeyer
Photoacoustic imaging (PAI) is an attractive imaging modality that can volumetrically map the distribution of photoabsorbing molecules with deeper tissue penetration than multiphoton microscopy. To enable dynamic sensing of divalent cations via PAI, we have engineered a new reversible near infrared probe that is more sensitive to calcium as compared to other biologically relevant cations. The metallochromic compound showed a strong reduction of its peak absorbance at 765 nm upon addition of calcium ions that was translated into robust signal changes in photoacoustic images...
October 25, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27761409/multimodal-optoacoustic-and-multiphoton-microscopy-of-human-carotid-atheroma
#18
Markus Seeger, Angelos Karlas, Dominik Soliman, Jaroslav Pelisek, Vasilis Ntziachristos
Carotid artery atherosclerosis is a main cause of stroke. Understanding atherosclerosis biology is critical in the development of targeted prevention and treatment strategies. Consequently, there is demand for advanced tools investigating atheroma pathology. We consider hybrid optoacoustic and multiphoton microscopy for the integrated and complementary interrogation of plaque tissue constituents and their mutual interactions. Herein, we visualize human carotid plaque using a hybrid multimodal imaging system that combines optical resolution optoacoustic (photoacoustic) microscopy, second and third harmonic generation microscopy, and two-photon excitation fluorescence microscopy...
September 2016: Photoacoustics
https://www.readbyqxmd.com/read/27747597/quantitative-multimodal-multiparametric-imaging-in-alzheimer-s-disease
#19
Qian Zhao, Xueqi Chen, Yun Zhou
Alzheimer's disease (AD) is a progressive neurodegenerative disorder, causing changes in memory, thinking, and other dysfunction of brain functions. More and more people are suffering from the disease. Early neuroimaging techniques of AD are needed to develop. This review provides a preliminary summary of the various neuroimaging techniques that have been explored for in vivo imaging of AD. Recent advances in magnetic resonance (MR) techniques, such as functional MR imaging (fMRI) and diffusion MRI, give opportunities to display not only anatomy and atrophy of the medial temporal lobe, but also at microstructural alterations or perfusion disturbance within the AD lesions...
March 2016: Brain Informatics
https://www.readbyqxmd.com/read/27732879/temporal-and-spatial-analysis-of-stromal-cell-and-extracellular-matrix-patterning-following-lamellar-keratectomy
#20
Pouriska B Kivanany, Kyle C Grose, W Matthew Petroll
Extracellular matrix (ECM) supplies both physical and chemical signals to keratocytes which can impact their differentiation to fibroblasts and/or myofibroblasts. It also provides a substrate through which they migrate during wound repair. We have previously shown that following transcorneal freeze injury (FI), migrating corneal fibroblasts align parallel to the stromal lamellae during wound repopulation. In this study, we compare cell and ECM patterning both within and on top of the stroma at different time points following lamellar keratectomy (LK) in the rabbit...
October 11, 2016: Experimental Eye Research
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