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multiphoton imaging

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https://www.readbyqxmd.com/read/28634334/image-guided-transplantation-of-single-cells-in-the-bone-marrow-of-live-animals
#1
Raphaël Turcotte, Clemens Alt, Judith M Runnels, Kyoko Ito, Juwell W Wu, Walid Zaher, Luke J Mortensen, Lev Silberstein, Daniel C Côté, Andrew L Kung, Keisuke Ito, Charles P Lin
Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28628128/adaptive-optics-improves-multiphoton-super-resolution-imaging
#2
Wei Zheng, Yicong Wu, Peter Winter, Robert Fischer, Damian Dalle Nogare, Amy Hong, Chad McCormick, Ryan Christensen, William P Dempsey, Don B Arnold, Joshua Zimmerberg, Ajay Chitnis, James Sellers, Clare Waterman, Hari Shroff
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface.
June 19, 2017: Nature Methods
https://www.readbyqxmd.com/read/28625344/confined-photonic-mode-propagation-observed-in-photoemission-electron-microscopy
#3
Theodore Stenmark, R C Word, R Könenkamp
Using photoemission electron microscopy (PEEM) we present a comparative analysis of the wavelength dependence of propagating fields in a simple optical slab waveguide and a thin film photonic crystal W1-type waveguide. We utilize an interferometric imaging approach for light in the near-ultraviolet regime where a 2-photon process is required to produce photoelectron emission. The typical spatial resolution in these experiments is < 30nm. Electromagnetic theory and finite element simulations are shown to be in good agreement with the experimental observations...
June 13, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28624539/polarization-resolved-second-harmonic-microscopy
#4
REVIEW
Nirmal Mazumder, Gitanjal Deka, Wei-Wen Wu, Ankur Gogoi, Guan-Yu Zhuo, Fu-Jen Kao
Second harmonic (SH) microscopy has proven to be a powerful imaging modality over the past years due to its intrinsic advantages as a multiphoton process with endogenous contrast specificity, which allows pinhole-less optical sectioning, non-invasive observation, deep tissue penetration, and the possibility of easier signal detection at visible wavelengths. Depending on the relative orientation between the polarization of the incoming light and the second-order susceptibility of non-centrosymmetric structures, SH microscopy provides the unique capacity to probe the absolute molecular structure of a broad variety of biological tissues without the necessity for additional labeling...
June 14, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28596141/delivery-of-cyclodextrin-polymers-to-bacterial-biofilms-an-exploratory-study-using-rhodamine-labelled-cyclodextrins-and-multiphoton-microscopy
#5
Hanna Thomsen, Gábor Benkovics, Éva Fenyvesi, Anne Farewell, Milo Malanga, Marica B Ericson
Cyclodextrin (CD) polymers are interesting nanoparticulate systems for pharmaceutical delivery; however, knowledge regarding their applications towards delivery into complex microbial biofilm structures is so far limited. The challenge is to demonstrate penetration and transport through the biofilm and its exopolysaccharide matrix. The ideal functionalization for penetration into mature biofilms is unexplored. In this paper, we present a novel set of rhodamine labelled βCD-polymers, with different charge moieties, i...
June 6, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28580960/rapid-visualization-of-grain-boundaries-in-monolayer-mos2-by-multiphoton-microscopy
#6
Lasse Karvonen, Antti Säynätjoki, Mikko J Huttunen, Anton Autere, Babak Amirsolaimani, Shisheng Li, Robert A Norwood, Nasser Peyghambarian, Harri Lipsanen, Goki Eda, Khanh Kieu, Zhipei Sun
Grain boundaries have a major effect on the physical properties of two-dimensional layered materials. Therefore, it is important to develop simple, fast and sensitive characterization methods to visualize grain boundaries. Conventional Raman and photoluminescence methods have been used for detecting grain boundaries; however, these techniques are better suited for detection of grain boundaries with a large crystal axis rotation between neighbouring grains. Here we show rapid visualization of grain boundaries in chemical vapour deposited monolayer MoS2 samples with multiphoton microscopy...
June 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/28569799/femtosecond-response-of-polyatomic-molecules-to-ultra-intense-hard-x-rays
#7
A Rudenko, L Inhester, K Hanasaki, X Li, S J Robatjazi, B Erk, R Boll, K Toyota, Y Hao, O Vendrell, C Bomme, E Savelyev, B Rudek, L Foucar, S H Southworth, C S Lehmann, B Kraessig, T Marchenko, M Simon, K Ueda, K R Ferguson, M Bucher, T Gorkhover, S Carron, R Alonso-Mori, J E Koglin, J Correa, G J Williams, S Boutet, L Young, C Bostedt, S-K Son, R Santra, D Rolles
X-ray free-electron lasers enable the investigation of the structure and dynamics of diverse systems, including atoms, molecules, nanocrystals and single bioparticles, under extreme conditions. Many imaging applications that target biological systems and complex materials use hard X-ray pulses with extremely high peak intensities (exceeding 10(20) watts per square centimetre). However, fundamental investigations have focused mainly on the individual response of atoms and small molecules using soft X-rays with much lower intensities...
June 1, 2017: Nature
https://www.readbyqxmd.com/read/28558136/multiphoton-direct-laser-writing-and-3d-imaging-of-polymeric-freestanding-architectures-for-cell-colonization
#8
Angelo Accardo, Marie-Charline Blatché, Rémi Courson, Isabelle Loubinoux, Christophe Thibault, Laurent Malaquin, Christophe Vieu
The realization of 3D architectures for the study of cell growth, proliferation, and differentiation is a task of fundamental importance for both technological and biological communities involved in the development of biomimetic cell culture environments. Here we report the fabrication of 3D freestanding scaffolds, realized by multiphoton direct laser writing and seeded with neuroblastoma cells, and their multitechnique characterization using advanced 3D fluorescence imaging approaches. The high accuracy of the fabrication process (≈200 nm) allows a much finer control of the micro- and nanoscale features compared to other 3D printing technologies based on fused deposition modeling, inkjet printing, selective laser sintering, or polyjet technology...
May 30, 2017: Small
https://www.readbyqxmd.com/read/28556893/a-component-by-component-characterisation-of-high-risk-atherosclerotic-plaques-by-multiphoton-microscopic-imaging
#9
M Jain, B Wu, D Pisapia, S Salvatore, S Mukherjee, N Narula
AIMS: Atherosclerotic plaques vulnerable to rupture are almost always inflamed, and carry a large lipid core covered by a thin fibrous cap. The other components may include neovascularisation, intraplaque haemorrhage and spotty calcification. In contrast, stable plaques are characterised by a predominance of smooth muscle cells and collagen, and lipid core is usually deep seated or absent. This study is a proof of principle experiment to evaluate the feasibility of multiphoton microscopy (MPM) to identify aforementioned plaque components...
May 30, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28542327/efficient-up-conversion-in-yb-er-nat-xo4-2-thermal-nanoprobes-imaging-of-their-distribution-in-a-perfused-mouse
#10
Carlos Zaldo, María Dolores Serrano, Xiumei Han, Concepción Cascales, Marta Cantero, Lluís Montoliu, Elvira Arza, Valeria R Caiolfa, Moreno Zamai
Yb and Er codoped NaT(XO4)2 (T = Y, La, Gd, Lu and X = Mo, W) disordered oxides show a green (Er3+ related) up-conversion (UC) efficiency comparable to that of Yb:Er:β-NaYF4 compound and unless 3 times larger UC ratiometric thermal sensitivity. The similar UC efficiency of Yb:Er doped NaT(XO4)2 and β-NaYF4 compounds allowed testing equal subcutaneous depths of ex-vivo chicken tissue in both cases. This extraordinary behavior for NaT(XO4)2 oxides with large cutoff phonon energy (ħω≈ 920 cm-1) is ascribed to 4F9/2 electron population recycling to higher energy 4G11/2 level by a phonon assisted transition...
2017: PloS One
https://www.readbyqxmd.com/read/28529998/complement-c5a-receptor-is-the-key-initiator-of-neutrophil-adhesion-igniting-immune-complex-induced-arthritis
#11
Yoshishige Miyabe, Chie Miyabe, Thomas T Murooka, Edward Y Kim, Gail A Newton, Nancy D Kim, Bodduluri Haribabu, Francis W Luscinskas, Thorsten R Mempel, Andrew D Luster
The deposition of immune complexes (IC) in tissues induces a "type III hypersensitivity" that results in tissue damage and underlies the pathogenesis of many autoimmune diseases. The neutrophil is the first immune cell recruited into sites of IC deposition and plays a critical role in shaping the overall tissue response. However, the mechanism by which IC initiate and propagate neutrophil infiltration into tissue is not known. Here, using intravital multiphoton joint imaging of IC-induced arthritis in live mice, we found that the complement C5a receptor (C5aR) was the key initiator of neutrophil adhesion on joint endothelium...
January 2017: Science Immunology
https://www.readbyqxmd.com/read/28528636/correlative-two-photon-and-serial-block-face-scanning-electron-microscopy-in-neuronal-tissue-using-3d-near-infrared-branding-maps
#12
Robert M Lees, Christopher J Peddie, Lucy M Collinson, Michael C Ashby, Paul Verkade
Linking cellular structure and function has always been a key goal of microscopy, but obtaining high resolution spatial and temporal information from the same specimen is a fundamental challenge. Two-photon (2P) microscopy allows imaging deep inside intact tissue, bringing great insight into the structural and functional dynamics of cells in their physiological environment. At the nanoscale, the complex ultrastructure of a cell's environment in tissue can be reconstructed in three dimensions (3D) using serial block face scanning electron microscopy (SBF-SEM)...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28499632/a-comprehensive-study-of-layer-specific-morphological-changes-in-the-microstructure-of-carotid-arteries-under-uniaxial-load
#13
Witold Krasny, Claire Morin, Hélène Magoariec, Stéphane Avril
The load bearing properties of large blood vessels are principally conferred by collagen and elastin networks and their microstructural organization plays an important role in the outcomes of various arterial pathologies. In particular, these fibrous networks are able to rearrange and reorient spatially during mechanical deformations. In this study, we investigate for the first time whether these well-known morphological rearrangements are the same across the whole thickness of blood vessels, and subsequently if the underlying mechanisms that govern these rearrangements can be predicted using affine kinematics...
May 9, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28497780/giant-five-photon-absorption-from-multidimensional-core-shell-halide-perovskite-colloidal-nanocrystals
#14
Weiqiang Chen, Saikat Bhaumik, Sjoerd A Veldhuis, Guichuan Xing, Qiang Xu, Michael Grätzel, Subodh Mhaisalkar, Nripan Mathews, Tze Chien Sum
Multiphoton absorption processes enable many technologically important applications, such as in vivo imaging, photodynamic therapy and optical limiting, and so on. Specifically, higher-order nonlinear absorption such as five-photon absorption offers significant advantages of greater spatial confinement, increased penetration depth, reduced autofluorescence, enhanced sensitivity and improved resolution over lower orders in bioimaging. Organic chromophores and conventional semiconductor nanocrystals are leaders in two-/three-photon absorption applications, but face considerable challenges from their small five-photon action cross-sections...
May 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28487023/redefining-3dimensional-placental-membrane-microarchitecture-using-multiphoton-microscopy-and-optical-clearing
#15
Lauren Richardson, Gracie Vargas, Tyra Brown, Lorenzo Ochoa, Jayshil Trivedi, Marian Kacerovský, Martha Lappas, Ramkumar Menon
INTRODUCTION: Remodeling of human placental membranes (amniochorionic or fetalmembrane) throughout gestation, a necessity to accommodate increasing uterine volume, involves continuous alterations (replacement of cells and remodeling of extracellular matrix). Methodologic limitations have obscured microscopic determination of cellular and layer-level alterations. This study used a combination of advanced imaging by multiphoton autofluorescence microscopy (MPAM) and second harmonic generation (SHG) microscopy along with tissue optical clearing to characterize the 3Dimensional multilayer organization of placental membranes...
May 2017: Placenta
https://www.readbyqxmd.com/read/28473723/high-resolution-morphological-approach-to-analyse-elastic-laminae-injuries-of-the-ascending-aorta-in-a-murine-model-of-marfan-syndrome
#16
Júlia López-Guimet, Jordi Andilla, Pablo Loza-Alvarez, Gustavo Egea
In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited information about the precise location, progression and 3D extension of the microstructural changes that occur in each lamina. We implemented a method using multiphoton excitation fluorescence microscopy and computational image processing, which provides high-resolution en-face images of segmented individual laminae from unstained whole aortic samples...
May 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28470531/assessment-of-population-and-ecm-production-using-multiphoton-microscopy-as-an-indicator-of-cell-viability
#17
Martin Vielreicher, Oliver Friedrich
Multiphoton microscopy allows continuous depth-resolved, nondestructive imaging of scaffold-seeded cells during cell or tissue culture. Spectrally separated images in high resolution can be provided while cells are conserved in their native state. Here we describe the seeding of mesenchymal stem cells to bacterial nanocellulose hydropolymer scaffolds followed by 2-channel imaging of cellular autofluorescence (AF) and collagen-I formation using second harmonic generation (SHG) signals. With this approach the simultaneous observation of the progression of cell morphology and production of extracellular matrix as hallmarks of viability and cell fitness is possible...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28468438/multiphoton-imaging-with-blue-diode-pumped-sesam-modelocked-ti-sapphire-oscillator-generating-5-nj-82-fs-pulses
#18
Andreas Rohrbacher, Omar E Olarte, Vesna Villamaina, Pablo Loza-Alvarez, Bojan Resan
We report a directly blue diode pumped Ti:Sapphire oscillator that generates 5 nJ pulses. This is five times higher pulse energy than previously reported for a directly diode pumped Ti:sapphire laser. With 460 mW of average power at 92 MHz and 82 fs pulses, its peak power reaches 61 kW, also several times higher the value than previously published. Direct diode pumping significantly reduces the complexity and therefore the footprint and the cost of the laser, while SESAM modelocking ensures reliable selfstarting and robust operation...
May 1, 2017: Optics Express
https://www.readbyqxmd.com/read/28464515/rapid-label-free-identification-of-cerebellar-structures-using-multiphoton-microscopy
#19
Shu Wang, Xiuqiang Chen, Weilin Wu, Zhida Chen, Huiping Du, Xingfu Wang, Yu Vincent Fu, Liwen Hu, Jianxin Chen
The cerebellum is the prominent laminar structure of the mammalian brain that has been implicated in various psychiatric and neurological diseases. Although clinical brain imaging techniques have provided precise anatomic images of cerebellar structures, a definitive diagnosis still requires adequate resolution to identify individual layers in cerebellar cortex, the extent of tumor, even requires the histological tissue examination during surgical procedures. In this study, multiphoton microscopy (MPM), based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), was perform on the rat cerebellar structures and pathology with the combination of image analysis methods...
May 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28464488/temporal-focusing-based-widefield-multiphoton-microscopy-with-spatially-modulated-illumination-for-biotissue-imaging
#20
Chia-Yuan Chang, Cheng-Han Lin, Chun-Yu Lin, Yong-Da Sie, Yvonne Yuling Hu, Sheng-Feng Tsai, Shean-Jen Chen
A developed temporal focusing-based multiphoton excitation microscope (TFMPEM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPEM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPEM can be condensed from 3...
May 2, 2017: Journal of Biophotonics
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