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multiphoton imaging

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https://www.readbyqxmd.com/read/28542327/efficient-up-conversion-in-yb-er-nat-xo4-2-thermal-nanoprobes-imaging-of-their-distribution-in-a-perfused-mouse
#1
Carlos Zaldo, María Dolores Serrano, Xiumei Han, Concepción Cascales, Marta Cantero, Lluís Montoliu, Elvira Arza, Valeria R Caiolfa, Moreno Zamai
Yb and Er codoped NaT(XO4)2 (T = Y, La, Gd, Lu and X = Mo, W) disordered oxides show a green (Er3+ related) up-conversion (UC) efficiency comparable to that of Yb:Er:β-NaYF4 compound and unless 3 times larger UC ratiometric thermal sensitivity. The similar UC efficiency of Yb:Er doped NaT(XO4)2 and β-NaYF4 compounds allowed testing equal subcutaneous depths of ex-vivo chicken tissue in both cases. This extraordinary behavior for NaT(XO4)2 oxides with large cutoff phonon energy (ħω≈ 920 cm-1) is ascribed to 4F9/2 electron population recycling to higher energy 4G11/2 level by a phonon assisted transition...
2017: PloS One
https://www.readbyqxmd.com/read/28529998/complement-c5a-receptor-is-the-key-initiator-of-neutrophil-adhesion-igniting-immune-complex-induced-arthritis
#2
Yoshishige Miyabe, Chie Miyabe, Thomas T Murooka, Edward Y Kim, Gail A Newton, Nancy D Kim, Bodduluri Haribabu, Francis W Luscinskas, Thorsten R Mempel, Andrew D Luster
The deposition of immune complexes (IC) in tissues induces a "type III hypersensitivity" that results in tissue damage and underlies the pathogenesis of many autoimmune diseases. The neutrophil is the first immune cell recruited into sites of IC deposition and plays a critical role in shaping the overall tissue response. However, the mechanism by which IC initiate and propagate neutrophil infiltration into tissue is not known. Here, using intravital multiphoton joint imaging of IC-induced arthritis in live mice, we found that the complement C5a receptor (C5aR) was the key initiator of neutrophil adhesion on joint endothelium...
January 2017: Science Immunology
https://www.readbyqxmd.com/read/28528636/correlative-two-photon-and-serial-block-face-scanning-electron-microscopy-in-neuronal-tissue-using-3d-near-infrared-branding-maps
#3
Robert M Lees, Christopher J Peddie, Lucy M Collinson, Michael C Ashby, Paul Verkade
Linking cellular structure and function has always been a key goal of microscopy, but obtaining high resolution spatial and temporal information from the same specimen is a fundamental challenge. Two-photon (2P) microscopy allows imaging deep inside intact tissue, bringing great insight into the structural and functional dynamics of cells in their physiological environment. At the nanoscale, the complex ultrastructure of a cell's environment in tissue can be reconstructed in three dimensions (3D) using serial block face scanning electron microscopy (SBF-SEM)...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28499632/a-comprehensive-study-of-layer-specific-morphological-changes-in-the-microstructure-of-carotid-arteries-under-uniaxial-load
#4
Witold Krasny, Claire Morin, Hélène Magoariec, Stéphane Avril
The load bearing properties of large blood vessels are principally conferred by collagen and elastin networks and their microstructural organization plays an important role in the outcomes of various arterial pathologies. In particular, these fibrous networks are able to rearrange and reorient spatially during mechanical deformations. In this study, we investigate for the first time whether these well-known morphological rearrangements are the same across the whole thickness of blood vessels, and subsequently if the underlying mechanisms that govern these rearrangements can be predicted using affine kinematics...
May 9, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28497780/giant-five-photon-absorption-from-multidimensional-core-shell-halide-perovskite-colloidal-nanocrystals
#5
Weiqiang Chen, Saikat Bhaumik, Sjoerd A Veldhuis, Guichuan Xing, Qiang Xu, Michael Grätzel, Subodh Mhaisalkar, Nripan Mathews, Tze Chien Sum
Multiphoton absorption processes enable many technologically important applications, such as in vivo imaging, photodynamic therapy and optical limiting, and so on. Specifically, higher-order nonlinear absorption such as five-photon absorption offers significant advantages of greater spatial confinement, increased penetration depth, reduced autofluorescence, enhanced sensitivity and improved resolution over lower orders in bioimaging. Organic chromophores and conventional semiconductor nanocrystals are leaders in two-/three-photon absorption applications, but face considerable challenges from their small five-photon action cross-sections...
May 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28487023/redefining-3dimensional-placental-membrane-microarchitecture-using-multiphoton-microscopy-and-optical-clearing
#6
Lauren Richardson, Gracie Vargas, Tyra Brown, Lorenzo Ochoa, Jayshil Trivedi, Marian Kacerovský, Martha Lappas, Ramkumar Menon
INTRODUCTION: Remodeling of human placental membranes (amniochorionic or fetalmembrane) throughout gestation, a necessity to accommodate increasing uterine volume, involves continuous alterations (replacement of cells and remodeling of extracellular matrix). Methodologic limitations have obscured microscopic determination of cellular and layer-level alterations. This study used a combination of advanced imaging by multiphoton autofluorescence microscopy (MPAM) and second harmonic generation (SHG) microscopy along with tissue optical clearing to characterize the 3Dimensional multilayer organization of placental membranes...
May 2017: Placenta
https://www.readbyqxmd.com/read/28473723/high-resolution-morphological-approach-to-analyse-elastic-laminae-injuries-of-the-ascending-aorta-in-a-murine-model-of-marfan-syndrome
#7
Júlia López-Guimet, Jordi Andilla, Pablo Loza-Alvarez, Gustavo Egea
In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited information about the precise location, progression and 3D extension of the microstructural changes that occur in each lamina. We implemented a method using multiphoton excitation fluorescence microscopy and computational image processing, which provides high-resolution en-face images of segmented individual laminae from unstained whole aortic samples...
May 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28470531/assessment-of-population-and-ecm-production-using-multiphoton-microscopy-as-an-indicator-of-cell-viability
#8
Martin Vielreicher, Oliver Friedrich
Multiphoton microscopy allows continuous depth-resolved, nondestructive imaging of scaffold-seeded cells during cell or tissue culture. Spectrally separated images in high resolution can be provided while cells are conserved in their native state. Here we describe the seeding of mesenchymal stem cells to bacterial nanocellulose hydropolymer scaffolds followed by 2-channel imaging of cellular autofluorescence (AF) and collagen-I formation using second harmonic generation (SHG) signals. With this approach the simultaneous observation of the progression of cell morphology and production of extracellular matrix as hallmarks of viability and cell fitness is possible...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28468438/multiphoton-imaging-with-blue-diode-pumped-sesam-modelocked-ti-sapphire-oscillator-generating-5-nj-82-fs-pulses
#9
Andreas Rohrbacher, Omar E Olarte, Vesna Villamaina, Pablo Loza-Alvarez, Bojan Resan
We report a directly blue diode pumped Ti:Sapphire oscillator that generates 5 nJ pulses. This is five times higher pulse energy than previously reported for a directly diode pumped Ti:sapphire laser. With 460 mW of average power at 92 MHz and 82 fs pulses, its peak power reaches 61 kW, also several times higher the value than previously published. Direct diode pumping significantly reduces the complexity and therefore the footprint and the cost of the laser, while SESAM modelocking ensures reliable selfstarting and robust operation...
May 1, 2017: Optics Express
https://www.readbyqxmd.com/read/28464515/rapid-label-free-identification-of-cerebellar-structures-using-multiphoton-microscopy
#10
Shu Wang, Xiuqiang Chen, Weilin Wu, Zhida Chen, Huiping Du, Xingfu Wang, Yu Vincent Fu, Liwen Hu, Jianxin Chen
The cerebellum is the prominent laminar structure of the mammalian brain that has been implicated in various psychiatric and neurological diseases. Although clinical brain imaging techniques have provided precise anatomic images of cerebellar structures, a definitive diagnosis still requires adequate resolution to identify individual layers in cerebellar cortex, the extent of tumor, even requires the histological tissue examination during surgical procedures. In this study, multiphoton microscopy (MPM), based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), was perform on the rat cerebellar structures and pathology with the combination of image analysis methods...
May 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28464488/temporal-focusing-based-widefield-multiphoton-microscopy-with-spatially-modulated-illumination-for-biotissue-imaging
#11
Chia-Yuan Chang, Cheng-Han Lin, Chun-Yu Lin, Yong-Da Sie, Yvonne Yuling Hu, Sheng-Feng Tsai, Shean-Jen Chen
A developed temporal focusing-based multiphoton excitation microscope (TFMPEM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPEM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPEM can be condensed from 3...
May 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28462391/the-impact-of-electrographic-seizures-on-developing-hippocampal-dendrites-is-calcineurin-dependent
#12
Masataka Nishimura, J R Casanova, John W Swann
Neurobehavioral abnormalities are commonly associated with intractable childhood epilepsy. Studies from numerous labs have demonstrated cognitive and socialization deficits in rats and mice that have experienced early-life seizures. However, the cellular and molecular mechanisms underlying these effects are unknown. Previously, experiments have shown that recurrent seizures in infancy suppress the growth of hippocampal dendrites at the same time they impair learning and memory. Experiments in slice cultures have also demonstrated dendrite growth suppression...
March 2017: ENeuro
https://www.readbyqxmd.com/read/28455851/fragmentation-behavior-of-dota-complexes-under-different-activation-conditions
#13
Sebastian Beck, Sabrina Trog, Sarah Knizia, Michael W Linscheid
1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) is a macrocycle that is able to complexate a large variety of metals. The stability of these metal complexes is generally very high. This has led to the use of these complexes in medical applications, e.g. magnetic resonance imaging. Here, we have investigated the fragmentation behavior of a number of DOTA-metal complexes under collision-induced dissociation (CID), infrared-multiphoton dissociation (IRMPD) and higher-energy collisional dissociation (HCD) activation conditions...
April 28, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28434997/multi-photon-microscopy-in-cardiovascular-research
#14
REVIEW
Zhuojun Wu, Timo Rademakers, Fabian Kiessling, Michael Vogt, Erik Westein, Christian Weber, Remco Ta Megens, Marc van Zandvoort
Multiphoton laser scanning microscopy has proven profound value for ex vivo 3D histology and in vivo imaging of motionless tissue. The development of triggering systems and fast imaging methods, combined with advanced preparation procedures solved the challenging task of intravital imaging of the fast pulsating heart and major arteries in animals and further increased the popularity of intravital multiphoton imaging in cardiovascular research. This review article will highlight the potential of multiphoton microscopy for the visualization and characterization of dynamical and structural processes involved in cardiac and vascular diseases, both in an ex vivo and an intravital animal setting...
April 18, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28434905/using-quantitative-intravital-multiphoton-microscopy-to-dissect-hepatic-transport-in-rats
#15
Kenneth W Dunn, Jennifer C Ryan
Hepatic solute transport is a complex process whose disruption is associated with liver disease and drug-induced liver injury. Intravital multiphoton fluorescence excitation microscopy provides the spatial and temporal resolution necessary to characterize hepatic transport at the level of individual hepatocytes in vivo and thus to identify the mechanisms and cellular consequences of cholestasis. Here we present an overview of the use of fluorescence microscopy for studies of hepatic transport in living animals, and describe how we have combined methods of intravital microscopy and digital image analysis to dissect the effects of drugs and pathological conditions on the function of hepatic transporters in vivo...
April 20, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28422363/the-occhiolino-occ-mutant-zebrafish-a-model-for-development-of-the-optical-function-in-the-biological-lens
#16
Masamoto Aose, Tor H Linbo, Owen Lawrence, Tadashi Senoo, David W Raible, John I Clark
BACKGROUND: Zebrafish visual function depends on quality optics. An F3 screen for developmental mutations in the zebrafish nervous system was conducted in wild-type (wt) AB zebrafish exposed to 3 mM of N-ethyl-N-nitrosourea (ENU). RESULTS: Mutant offspring, identified in an F3 screen, were characterized by a small pupil, resulting from retinal hypertrophy or hyperplasia and a small lens. Deficits in visual function made feeding difficult after hatching at approximately 5-6 dpf (days post fertilization)...
April 19, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28420873/viewing-immune-regulation-as-it-happens-in-vivo-imaging-for-investigation-of-regulatory-t-cell-function
#17
REVIEW
Michael J Hickey, Zachary Chow
Regulatory T cells (Tregs) play indispensable roles in the immune system, in limiting excessive or inappropriate immune and inflammatory responses. They achieve this function via effects on other immune cells in the secondary lymphoid system, and in peripheral locations such as skin, gut and bone marrow. As for the more extensively-studied cellular players in the immune system, particularly dendritic cells and conventional T cells, in vivo imaging of Tregs via two-photon or multiphoton microscopy (MPM) has been central to the development of understanding how these cells function...
April 19, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28397809/second-harmonic-generation-imaging-of-corneal-stroma-after-infection-by-pseudomonas-aeruginosa
#18
Danielle M Robertson, Nathan A Rogers, W Matthew Petroll, Meifang Zhu
Pseudomonas aeruginosa is a pathogenic gram-negative organism that has the ability to cause blinding corneal infections following trauma and during contact lens wear. In this study, we investigated the directional movement and orientation of an invasive corneal isolate of P. aeruginosa in the corneal stroma during infection of ex vivo and in vivo rabbit corneas using multiphoton fluorescence and second harmonic generation (SHG) imaging. Ex vivo, rabbit corneas were subject to three partial thickness wounds prior to inoculation...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28389037/idiopathic-atrophoderma-of-pasini-and-pierini-a-case-study-of-collagen-and-elastin-texture-by-multiphoton-microscopy
#19
Gislaine Vieira-Damiani, Denise Lage, Patrícia Érica Christofoletti Daldon, Caroline Romanelli Tibúrcio Alves, Maria Letícia Cintra, Konradin Metze, Javier Adur, Vitor B Pelegati, Hernandes F Carvalho, Carlos Lenz Cesar
BACKGROUND: The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. OBJECTIVE: To identify histologic features that distinguish IAPP from unaffected skin...
April 4, 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28381061/order-of-magnitude-multiphoton-signal-enhancement-based-on-characterization-of-absorption-spectra-of-immersion-oils-at-the-1700-nm-window
#20
Ke Wang, Wenhui Wen, Yuxin Wang, Kai Wang, Jiexing He, Jiaqi Wang, Peng Zhai, Yanfu Yang, Ping Qiu
To enhance signal levels in multiphoton microscopy (MPM) at the deep-tissue excitation window (1600-1820 nm) with oil immersion, we demonstrate: First, the absorption spectra of several commonly immersion oils are characterized, which were unknown before. Second, new material with lower absorption based on mixing is proposed. Third, optimal selection of excitation wavelength within this window is proposed based on absorption spectra characterization. Second and third harmonic generation imaging of mouse tissue corroborate our selection: 1600-nm excitation leads to notable orders-of-magnitude increase in MPM signal, compared with 1700-nm excitation, enabling 200-µm imaging depth of mouse skin while 1700-nm excitation could resolve virtually no structure...
March 20, 2017: Optics Express
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