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multiphoton imaging

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https://www.readbyqxmd.com/read/29332131/assessment-of-human-corneas-prior-to-transplantation-using-high-resolution-two-photon-imaging
#1
Ana Batista, Hans Georg Breunig, Aisada König, Andreas Schindele, Tobias Hager, Berthold Seitz, António Miguel Morgado, Karsten König
Purpose: The purpose of this study was to evaluate the feasibility of using two-photon imaging (TPI) to assess the condition of human corneas for transplantation. Methods: Human corneas were imaged after different storage times: short-term (STS), medium-term (MTS), and long-term (LTS) storage. A high-resolution, custom-built 5-dimensional multiphoton microscope with 12-fs pulsed laser excitation was used for image acquisition. Results: Optical discrimination between different corneal layers and sublayers based on their morphologic characteristics revealed by two-photon autofluorescence (AF) is possible...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29327927/atom-dependent-edge-enhanced-second-harmonic-generation-on-mos2-monolayers
#2
Kuang-I Lin, Yen-Hung Ho, Shu-Bai Liu, Jian-Jhih Ciou, Bo-Ting Huang, Christopher Chen, Han-Ching Chang, Chien-Liang Tu, Chang-Hsiao Chen
Edge morphology and lattice orientation of single-crystal molybdenum disulfide (MoS2) monolayers, a transition metal dichalcogenide (TMD), possessing a triangular shape with different edges grown by chemical vapor deposition are characterized by atomic force microscopy and transmission electron microscopy. Multiphoton laser scanning microscopy is utilized to study one-dimensional atomic edges of MoS2 monolayers with localized mid-gap electronic states, which result in greatly enhanced optical second-harmonic generation (SHG)...
January 12, 2018: Nano Letters
https://www.readbyqxmd.com/read/29319936/submucosal-gland-mucus-strand-velocity-is-decreased-in-chronic-rhinosinusitis
#3
Kiranya E Tipirneni, Shaoyan Zhang, Do-Yeon Cho, Jessica Grayson, Daniel F Skinner, Calvin Mackey, Lindsay Moore, Denzel Cole, Catherine G Banks, Bradford A Woodworth
BACKGROUND: Chronic rhinosinusitis (CRS) may be initiated by innately impaired host defense mechanisms that predispose the upper airways to infection. Recent evidence suggests tethering of submucosal gland mucus strands represents an inciting event within cystic fibrosis (CF) airways, occurring prior to onset of chronic infection. Submucosal gland hypertrophy and defective mucociliary clearance (MCC) are present in actively inflamed sinuses, but mucus strand velocity may also be affected as a secondary event, further contributing to chronic disease...
January 10, 2018: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/29311591/segmented-cell-analyses-to-measure-redox-states-of-autofluorescent-nad-p-h-fad-trp-in-cancer-cells-by-flim
#4
Horst Wallrabe, Zdenek Svindrych, Shagufta R Alam, Karsten H Siller, Tianxiong Wang, David Kashatus, Song Hu, Ammasi Periasamy
Multiphoton FLIM microscopy offers many opportunities to investigate processes in live cells, tissue and animal model systems. For redox measurements, FLIM data is mostly published by cell mean values and intensity-based redox ratios. Our method is based entirely on FLIM parameters generated by 3-detector time domain microscopy capturing autofluorescent signals of NAD(P)H, FAD and novel FLIM-FRET application of Tryptophan and NAD(P)H-a2%/FAD-a1% redox ratio. Furthermore, image data is analyzed in segmented cells thresholded by 2 × 2 pixel Regions of Interest (ROIs) to separate mitochondrial oxidative phosphorylation from cytosolic glycolysis in a prostate cancer cell line...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29306282/imaging-spectroscopy-of-the-missing-rempi-bands-of-methyl-radicals-final-touches-on-all-vibrational-frequencies-of-the-3p-rydberg-states
#5
Huilin Pan, Kopin Liu
(2 + 1) resonance-enhanced multiphoton ionization (REMPI) detection of methyl radicals, in particular that via the intermediate 3p Rydberg states, has shown to be a powerful method and thus enjoyed a wide range of applications. Methyl has six vibrational modes. Among them-including partially and fully deuterated isotopologs-four out of twenty vibrational frequencies in the intermediate 3p states have so far eluded direct spectroscopic determination. Here, by exploiting the imaging spectroscopy approach to a few judiciously selected chemical reactions, the four long-sought REMPI bands-CHD2(611), CH2D(311), CH2D(511), and CH2D(611)-are discovered, which complete the REMPI identification for probing any vibrational mode of excitation of methyl radical and its isotopologs...
January 7, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29303582/spectroscopic-study-of-a-photoactive-antibacterial-agent-2-3-distyrylindole
#6
Ruwini Dinushika Rajapaksha, Matthias Banet, Cody Champion, Liliya V Frolova, Snezna Rogelj, Pabitra Choudhury, Mahinda Iroshana Ranasinghe
Optical properties and fluorescence decay dynamics of a photoactive indole based antibacterial chromophore system, 2, 3-distyrylindole (23DSI), was investigated using various spectroscopic characterization techniques. These studies were done by utilizing the steady state UV-Vis spectroscopy, the steady state fluorescence spectroscopy, the time-resolve fluorescence upconversion spectroscopy, and time correlated single photon counting spectroscopy as experiment techniques. Our studies show that the 23DSI molecule has a multiphoton absorbing property as indicated by two and three photon absorption in both solution and solid phases...
January 5, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29289926/the-use-of-suction-blisters-to-measure-sunscreen-protection-against-uvr-induced-dna-damage
#7
Gwendal Josse, Thierry Douki, Jimmy Le Digabel, Eleonore Gravier, Emmanuel Questel
The formation of DNA photoproducts caused by solar UVR exposure needs to be investigated in-vivo and in particular in order to assess sunscreens' level of protection against solar genotoxicity. The study's purposes were: i) to evaluate if the roof of suction blisters is an appropriate sampling method for measuring photoproducts, and ii) to measure in-vivo sunscreen protection against cyclobutane pyrimidine dimers. Skin areas on the interior forearms of eight healthy volunteers were exposed in-vivo to 2 MED of simulated solar radiation (SSR) and to 15 MED on a sunscreen protected area...
December 22, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29287105/effect-of-intra-articular-administration-of-superparamagnetic-iron-oxide-nanoparticles-spions-for-mri-assessment-of-the-cartilage-barrier-in-a-large-animal-model
#8
Raphael Labens, Carola Daniel, Sarah Hall, Xin-Rui Xia, Tobias Schwarz
Early diagnosis of cartilage disease at a time when changes are limited to depletion of extracellular matrix components represents an important diagnostic target to reduce patient morbidity. This report is to present proof of concept for nanoparticle dependent cartilage barrier imaging in a large animal model including the use of clinical magnetic resonance imaging (MRI). Conditioned (following matrix depletion) and unconditioned porcine metacarpophalangeal cartilage was evaluated on the basis of fluorophore conjugated 30 nm and 80 nm spherical gold nanoparticle permeation and multiphoton laser scanning and bright field microscopy after autometallographic particle enhancement...
2017: PloS One
https://www.readbyqxmd.com/read/29286393/three-dimensional-reconstruction-of-the-vascular-architecture-of-the-passive-clarity-cleared-mouse-ovary
#9
Wei Hu, Amin Tamadon, Aaron J W Hsueh, Yi Feng
The ovary is the main organ of the female reproductive system and is essential for the production of female gametes and for controlling the endocrine system, but the complex structural relationships and three-dimensional (3D) vasculature architectures of the ovary are not well described. In order to visualize the 3D connections and architecture of blood vessels in the intact ovary, the first important step is to make the ovary optically clear. In order to avoid tissue shrinkage, we used the hydrogel fixation-based passive CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/ Immunostaining/In situ-hybridization-compatible Tissue Hydrogel) protocol method to clear an intact ovary...
December 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29251351/3d-stromal-tissue-equivalent-affects-intestinal-epithelium-morphogenesis-in-vitro
#10
Vincenza De Gregorio, Giorgia Imparato, Francesco Urciuolo, Paolo A Netti
Current in vitro models of human intestine commonly fail to mimic the complex intestinal functions and features required for drug development and disease research. Here we deeply investigate the interaction existing between epithelium and the underneath stroma, and its role in the epithelium morphogenesis. We cultured human intestinal subepithelial myofibroblasts (ISEMFs) in two different 3D configurations: 3D-collagen gel equivalent (3D-CGE) and 3D cell-synthetized stromal equivalent (3D-CSSE). The 3D-CGEs were obtained by means of the traditional collagen-based cell technique and the 3D-CSSE were obtained by bottom-up tissue engineering strategy...
December 18, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29249662/experience-dependent-plasticity-of-odor-representations-in-the-telencephalon-of-zebrafish
#11
Gilad A Jacobson, Peter Rupprecht, Rainer W Friedrich
Sensory systems balance stability and plasticity to optimize stimulus representations in dynamic environments. We studied these processes in the olfactory system of adult zebrafish. Activity patterns evoked by repeated odor stimulation were measured by multiphoton calcium imaging in the olfactory bulb (OB) and in telencephalic area Dp, the homolog of olfactory cortex. Whereas odor responses in the OB were highly reproducible, responses of Dp neurons adapted over trials and exhibited substantial variability that could be attributed to ongoing activity and to systematic changes in neuronal representations following each stimulus...
December 7, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29245768/non-bleaching-fluorescence-emission-difference-microscopy-using-single-808-nm-laser-excited-red-upconversion-emission
#12
Qiusheng Wu, Bingru Huang, Xingyun Peng, Sailing He, Qiuqiang Zhan
Optical super-resolution microscopy has become a powerful technique to help scientists to monitor the sample of interest at nanoscale. Fluorescence emission difference (FED) microscopy, a very facile super-resolution method, does not require high depleting laser intensity and is independent on the species of agents, which makes FED microscopy possess great potential. However, to date, the biomarkers applied in FED microscopy usually suffer from a photo-bleaching problem. In this work, by introducing Er3+ activated upconverting nanoparticles with red-color emission and non-photobleaching properties, we demonstrate nonbleaching super-resolution imaging with FED microscopy...
December 11, 2017: Optics Express
https://www.readbyqxmd.com/read/29219617/exploring-multiphoton-microscopy-as-a-novel-tool-to-differentiate-chromophobe-renal-cell-carcinoma-from-oncocytoma-in-fixed-tissue-sections
#13
Manu Jain, Brian D Robinson, Binlin Wu, Francesca Khani, Sushmita Mukherjee
CONTEXT: - Distinguishing chromophobe renal cell carcinoma (chRCC), especially in the presence of eosinophilic cytoplasm, from oncocytoma on hematoxylin-eosin can be difficult and often requires time-consuming ancillary procedures that ultimately may not be informative. OBJECTIVE: - To explore the potential of multiphoton microscopy (MPM) as an alternative and rapid diagnostic tool in differentiating oncocytoma from chRCC at subcellular resolution without tissue processing...
December 8, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29219514/second-harmonic-generation-of-unpolarized-light
#14
Changqin Ding, James R W Ulcickas, Fengyuan Deng, Garth J Simpson
A Mueller tensor mathematical framework was applied for predicting and interpreting the second harmonic generation (SHG) produced with an unpolarized fundamental beam. In deep tissue imaging through SHG and multiphoton fluorescence, partial or complete depolarization of the incident light complicates polarization analysis. The proposed framework has the distinct advantage of seamlessly merging the purely polarized theory based on the Jones or Cartesian susceptibility tensors with a more general Mueller tensor framework capable of handling partial depolarized fundamental and/or SHG produced...
November 10, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29217759/opposing-roles-of-dendritic-cell-subsets-in-experimental-gn
#15
Sebastian Brähler, Bernd H Zinselmeyer, Saravanan Raju, Maximilian Nitschke, Hani Suleiman, Brian T Saunders, Michael W Johnson, Alexander M C Böhner, Susanne F Viehmann, Derek J Theisen, Nicole M Kretzer, Carlos G Briseño, Konstantin Zaitsev, Olga Ornatsky, Qing Chang, Javier A Carrero, Jeffrey B Kopp, Maxim N Artyomov, Christian Kurts, Kenneth M Murphy, Jeffrey H Miner, Andrey S Shaw
Dendritic cells (DCs) are thought to form a dendritic network across barrier surfaces and throughout organs, including the kidney, to perform an important sentinel function. However, previous studies of DC function used markers, such as CD11c or CX3CR1, that are not unique to DCs. Here, we evaluated the role of DCs in renal inflammation using a CD11c reporter mouse line and two mouse lines with DC-specific reporters, Zbtb46-GFP and Snx22-GFP. Multiphoton microscopy of kidney sections confirmed that most of the dendritically shaped CD11c+ cells forming a network throughout the renal interstitium expressed macrophage-specific markers...
December 7, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29188660/multiphoton-fluorescence-lifetime-imaging-of-chemotherapy-distribution-in-solid-tumors
#16
Marjorie Carlson, Adrienne L Watson, Leah Anderson, David A Largaespada, Paolo P Provenzano
Doxorubicin is a commonly used chemotherapeutic employed to treat multiple human cancers, including numerous sarcomas and carcinomas. Furthermore, doxorubicin possesses strong fluorescent properties that make it an ideal reagent for modeling drug delivery by examining its distribution in cells and tissues. However, while doxorubicin fluorescence and lifetime have been imaged in live tissue, its behavior in archival samples that frequently result from drug and treatment studies in human and animal patients, and murine models of human cancer, has to date been largely unexplored...
November 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29188116/multimodal-nonlinear-optical-imaging-of-unstained-retinas-in-the-epi-direction-with-a-sub-40-fs-yb-fiber-laser
#17
Gabrielle A Murashova, Christopher A Mancuso, Jacob L Canfield, Sanae Sakami, Krzysztof Palczewski, Grazyna Palczewska, Marcos Dantus
Ultrafast lasers have potential use in ophthalmology for diagnoses through non-invasive imaging as well as for surgical therapies or for evaluating pharmacological therapies. New ultrafast laser sources, operating at 1.07 μm and sub-40 fs pulse durations, offer exciting possibilities in multiphoton imagining of the retina as the bulk of the eye is relatively transparent to this wavelength, three-photon excitation is not absorbed by DNA, and this wavelength has a greater penetration depth compared to the commonly used 800 nm Ti:Sapphire laser...
November 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29188103/comparison-of-second-harmonic-microscopy-images-of-collagen-based-ocular-tissues-with-800-and-1045-nm
#18
Juan M Bueno, Francisco J Ávila, Pablo Artal
Second harmonic generation (SHG) imaging is a well-suited multiphoton technique allowing visualization of biological tissues mainly composed of collagen with submicron resolution. Despite its inherent confocal properties, imaging of deeper layers within thick samples has still some limitations. Although the use of longer wavelengths might help to overcome this, the dependence between SHG signals and wavelength is still under discussion. We report here on the dependence with wavelength of SHG signals from collagen-based ocular tissues...
November 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29186380/impact-of-t-cell-mediated-immune-response-on-xenogeneic-heart-valve-transplantation-short-term-success-and-mid-term-failure
#19
Anna C Biermann, Julia Marzi, Eva Brauchle, Maria Schneider, Angela Kornberger, Sherif Abdelaziz, Julian L Wichmann, Christophe T Arendt, Eike Nagel, Kelvin G M Brockbank, Martina Seifert, Katja Schenke-Layland, Ulrich A Stock
OBJECTIVES: Allogeneic frozen cryopreserved heart valves (allografts or homografts) are commonly used in clinical practice. A major obstacle for their application is the limited availability in particular for paediatrics. Allogeneic large animal studies revealed that alternative ice-free cryopreservation (IFC) results in better matrix preservation and reduced immunogenicity. The objective of this study was to evaluate xenogeneic (porcine) compared with allogeneic (ovine) IFC heart valves in a large animal study...
November 27, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/29176571/health-state-dependent-multiphoton-induced-autofluorescence-in-human-3d-in-vitro-lung-cancer-model
#20
Vasyl Kilin, Christophe Mas, Samuel Constant, Jean-Pier Wolf, Luigi Bonacina
Lung diseases pose the highest risk of death and lung cancer is a top killer among cancers with a mortality rate up to 70% within 1 year after diagnosis. Such a fast escalation of this cancer development makes early diagnosis and treatment a highly challenging task, and currently there are no effective tools to diagnose the disease at an early stage. The ability to discriminate between healthy and tumorous tissue has made autofluorescence bronchoscopy a promising tool for detection of lung cancer; however, specificity of this method remains insufficiently low...
November 24, 2017: Scientific Reports
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