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https://www.readbyqxmd.com/read/28635332/50-ethanol-extract-of-orthosiphon-stamineus-modulates-genotoxicity-and-clastogenicity-induced-by-mitomycin-c
#1
Dhamraa Waleed Al-Dualimi, Aman Shah Abdul Majid, Sarah Furqan Faisal Al-Shimary, Amal Aziz Al-Saadi, Raghdaa Al Zarzour, Muhammad Asif, Chern Ein Oon, Amin Malik Shah Abdul Majid
Herbal products contain a variety of compounds which may be useful in protecting against cellular damage caused by mutagens. Orthosiphon stamineus (O.s) also known as Cat whiskers. The herb has been shown anti-oxidative properties and can modulate key cellular proteins that have cytoprotective effect. The study aimed to evaluate the effects of different doses (250, 500 and 1000 mg kg(-1)) of 50% ethanol extract of O.s (Et. O.s) on micro-nucleated polychromatic erythrocytes (MNPCE), Polychromatic to normachromatic erythrocytes ratio (PCE/NCE), Mitotic index (MI), and Chromosomal aberration (CA) in Bab/c mice...
June 21, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28630880/prominent-lateral-spread-of-imaged-evoked-activity-beyond-cortical-columns-in-barrel-cortex-provides-foundation-for-coding-whisker-identity
#2
Nathan S Jacobs, Ron D Frostig
The posterior medial barrel subfield (PMBSF) of a rat primary somatosensory cortex exquisitely demonstrates topography and columnar organization, defining features of sensory cortices in the mammalian brain. Optical imaging and neuronal recordings in rat PMBSF demonstrate how evoked cortical activity following single whisker stimulation also rapidly spreads laterally into surrounding cortices, disregarding columnar and modality boundaries. The current study quantifies the spatial prominence of such lateral activity spreads by demonstrating that functional connectivity between laterally spaced cortical locations is actually stronger than between vertically spaced cortical locations...
July 2017: Neurophotonics
https://www.readbyqxmd.com/read/28630879/imaging-cajal-s-neuronal-avalanche-how-wide-field-optical-imaging-of-the-point-spread-advanced-the-understanding-of-neocortical-structure-function-relationship
#3
REVIEW
Ron D Frostig, Cynthia H Chen-Bee, Brett A Johnson, Nathan S Jacobs
This review brings together a collection of studies that specifically use wide-field high-resolution mesoscopic level imaging techniques (intrinsic signal optical imaging; voltage-sensitive dye optical imaging) to image the cortical point spread (PS): the total spread of cortical activation comprising a large neuronal ensemble evoked by spatially restricted (point) stimulation of the sensory periphery (e.g., whisker, pure tone, point visual stimulation). The collective imaging findings, combined with supporting anatomical and electrophysiological findings, revealed some key aspects about the PS including its very large (radius of several mm) and relatively symmetrical spatial extent capable of crossing cytoarchitectural borders and trespassing into other cortical areas; its relationship with underlying evoked subthreshold activity and underlying anatomical system of long-range horizontal projections within gray matter, both also crossing borders; its contextual modulation and plasticity; the ability of its relative spatiotemporal profile to remain invariant to major changes in stimulation parameters; its potential role as a building block for integrative cortical activity; and its ubiquitous presence across various cortical areas and across mammalian species...
July 2017: Neurophotonics
https://www.readbyqxmd.com/read/28622442/muscular-basis-of-whisker-torsion-in-mice-and-rats
#4
Sebastian Haidarliu, Knarik Bagdasarian, Namrata Shinde, Ehud Ahissar
Whisking mammals move their whiskers in the rostrocaudal and dorsoventral directions with simultaneous rolling about their long axes (torsion). Whereas muscular control of the first two types of whisker movement was already established, the anatomic muscular substrate of the whisker torsion remains unclear. Specifically, it was not clear whether torsion is induced by asymmetrical operation of known muscles or by other largely unknown muscles. Here we report that mystacial pads of newborn and adult rats and mice contain oblique intrinsic muscles (OMs) that connect diagonally adjacent vibrissa follicles...
June 16, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28616371/tactile-sensing-with-whiskers-of-various-shapes-determining-the-three-dimensional-location-of-object-contact-based-on-mechanical-signals-at-the-whisker-base
#5
Lucie A Huet, John W Rudnicki, Mitra J Z Hartmann
Almost all mammals use their mystacial vibrissae (whiskers) as important tactile sensors. There are no sensors along the length of a whisker: all sensing is performed by mechanoreceptors at the whisker base. To use artificial whiskers as a sensing tool in robotics, it is essential to be able to determine the three-dimensional (3D) location at which a whisker has made contact with an object. With the assumption of quasistatic, frictionless, single-point contact, previous work demonstrated that the 3D contact point can be uniquely determined if all six components of force and moment are measured at the whisker base, but these measurements require a six-axis load cell...
June 1, 2017: Soft Robotics
https://www.readbyqxmd.com/read/28613155/dye-enhanced-visualization-of-rat-whiskers-for-behavioral-studies
#6
Jacopo Rigosa, Alessandro Lucantonio, Giovanni Noselli, Arash Fassihi, Erik Zorzin, Fabrizio Manzino, Francesca Pulecchi, Mathew E Diamond
Visualization and tracking of the facial whiskers is required in an increasing number of rodent studies. Though many approaches have been employed, only high-speed videography has proven adequate for measuring whisker motion and deformation during interaction with an object. However, whisker visualization and tracking is challenging for multiple reasons, primary among them the low contrast of the whisker against its background. Here we demonstrate a fluorescent dye method suitable for visualization of one or more rat whiskers...
June 14, 2017: ELife
https://www.readbyqxmd.com/read/28607423/growth-of-high-quality-bi2sr2-cacu2o8-%C3%AE-whiskers-and-electrical-properties-of-resulting-exfoliated-flakes
#7
Apoorv Jindal, Digambar A Jangade, Nikhil Kumar, Jaykumar Vaidya, Ipsita Das, Rudheer Bapat, Jayesh Parmar, Bhagyashree A Chalke, Arumugam Thamizhavel, Mandar M Deshmukh
In this work, we demonstrate a simple technique to grow high-quality whiskers of Bi2 Sr2 CaCu2 O8+δ - a high T c superconductor. Structural analysis shows the single-crystalline nature of the grown whiskers. To probe electrical properties, we exfoliate these whiskers into thin flakes (~50 nm thick) using the scotch-tape technique and develop a process to realize good electrical contacts. We observe a superconducting critical temperature, T c , of 86 K. We map the evolution of the critical current as a function of temperature...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28607173/tactile-defensiveness-and-impaired-adaptation-of-neuronal-activity-in-the-fmr1-knockout-mouse-model-of-autism
#8
Cynthia X He, Daniel A Cantu, Shilpa S Mantri, William A Zeiger, Anubhuti Goel, Carlos Portera-Cailliau
Sensory hypersensitivity is a common symptom in autism spectrum disorders (ASDs), including Fragile X Syndrome (FXS), and frequently leads to tactile defensiveness. In mouse models of ASDs, there is mounting evidence of neuronal and circuit hyperexcitability in several brain regions, which could contribute to sensory hypersensitivity. However, it is not yet known whether or how sensory stimulation might trigger abnormal sensory processing at the circuit level or abnormal behavioral responses in ASD mouse models, especially during an early developmental time when experience-dependent plasticity shapes such circuits...
June 12, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28605197/effects-of-nanochitin-on-enhancement-of-grain-yield-and-quality-of-winter-wheat
#9
Weijie Xue, Yanlai Han, Jinfang Tan, Yi Wang, Guowen Wang, Hezhong Wang
This study investigated the effects of different rates of nanochitin in soil on grain yield and quality of winter wheat. Nanochitin obtained by acidic hydrolysis of shrimp chitin was rod-like whisker possessing a hydrodynamic diameter of 143 nm and zeta potential of 55.7mV. Two varieties of winter wheat, multi spike wheat (MSW) and large spike wheat (LSW) were treated with nanochitin suspension in outside pot experiments. The results showed that 0.006 g·kg-1 of nanochitin in soil could significantly enhance the yield by 23...
June 12, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28602655/attention-robustly-gates-a-closed-loop-touch-reflex
#10
Dana Sherman, Tess Oram, David Harel, Ehud Ahissar
Rats' large whiskers (macrovibrissae) are used to explore their nearby environment, typically using repetitive protraction-retraction "whisking" motions that are coordinated with head and body movements [1-8]. Once objects are detected, the rat can further explore the object tactually by using both the macrovibrissae and an array of shorter, stationary microvibrissae on the chin, as well as by using the lips [9-11]. When touch occurs during whisking, a fast reflexive response, termed a touch-induced pump (TIP), may be triggered...
June 19, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28601594/zinc-oxide-crystal-whiskers-as-a-novel-sorbent-for-solid-phase-extraction-of-flavonoids
#11
Licheng Wang, Yangnan Shangguan, Xiudan Hou, Yong Jia, Shujuan Liu, Yingxin Sun, Yong Guo
As a novel solid-phase extraction material, zinc oxide crystal whiskers were used to extract flavonoid compounds and showed good extraction abilities. X-ray diffraction, scanning electron microscopy with energy dispersive X-ray spectroscopy and surface area/pore volume characterized the sorbent. The zinc oxide was packed into a solid-phase extraction micro-column and its extraction ability was evaluated by four model flavonoid compounds. The sample loading and elution parameters were optimized and the zinc oxide based analytical method for flavonoids was established...
May 31, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28597656/enhancing-mechanical-and-thermal-properties-of-epoxy-nanocomposites-via-alignment-of-magnetized-sic-whiskers
#12
James Townsend, Ruslan Burtovyy, Pavel Aprelev, Konstantin G Kornev, Igor Luzinov
This research is focused on the fabrication and properties of epoxy nanocomposites containing magnetized SiC whiskers (MSiCWs). To this end, we report an original strategy for fabrication of magnetically active SiCWs by decorating the whiskers with magnetic (iron oxide) nanoparticles via polymer-polymer (polyacrylic acid/poly(2-vinyl pyridine) complexation. The obtained whiskers demonstrated a substantial magnetic response in the polymerizing epoxy resin with application of only a 20 mT (200G) magnetic field...
June 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28591219/mechanisms-underlying-a-thalamocortical-transformation-during-active-tactile-sensation
#13
Diego Adrian Gutnisky, Jianing Yu, Samuel Andrew Hires, Minh-Son To, Michael Ross Bale, Karel Svoboda, David Golomb
During active somatosensation, neural signals expected from movement of the sensors are suppressed in the cortex, whereas information related to touch is enhanced. This tactile suppression underlies low-noise encoding of relevant tactile features and the brain's ability to make fine tactile discriminations. Layer (L) 4 excitatory neurons in the barrel cortex, the major target of the somatosensory thalamus (VPM), respond to touch, but have low spike rates and low sensitivity to the movement of whiskers. Most neurons in VPM respond to touch and also show an increase in spike rate with whisker movement...
June 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28588276/single-axon-level-morphological-analysis-of-corticofugal-projection-neurons-in-mouse-barrel-field
#14
Congdi Guo, Jie Peng, Yalun Zhang, Anan Li, Yuxin Li, Jing Yuan, Xiaofeng Xu, Miao Ren, Hui Gong, Shangbin Chen
Corticofugal projection neurons are key components in connecting the neocortex and the subcortical regions. In the barrel field, these neurons have various projection targets and play crucial roles in the rodent whisker sensorimotor system. However, the projection features of corticofugal projection neurons at the single-axon level are far from comprehensive elucidation. Based on a brain-wide positioning system with high-resolution imaging for Thy1-GFP M-line mice brains, we reconstructed and analyzed more than one hundred corticofugal projection neurons in both layer V and VI of barrel cortex...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28560274/properties-of-particle-phases-for-metal-matrix-composite-design
#15
C Baron, H Springer
Successful metallurgical design of metal-matrix-composites relies on the knowledge of the intrinsic property profiles of the metal matrix and especially the compounds employed for particles, whiskers or fibres. In this work we compiled the key properties melting point, bulk modulus, shear modulus, Young׳s modulus, density, hardness, Poisson׳s ratio and structure/space group from the widespread literature data for the most relevant compound types, i.e. borides, carbo-borides, carbides, oxides, nitrides and intermetallic phases...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28557664/efficacy-of-laser-photobiomodulation-on-morphological-and-functional-repair-of-the-facial-nerve
#16
Daniela Vieira Buchaim, Jesus Carlos Andreo, Rui Seabra Ferreira Junior, Benedito Barraviera, Antonio de Castro Rodrigues, Mariana de Cássia Macedo, Geraldo Marco Rosa Junior, Andre Luis Shinohara, Iris Jasmin Santos German, Karina Torres Pomini, Rogerio Leone Buchaim
OBJECTIVE: Evaluate the efficacy of low-level laser therapy (LLLT) on qualitative, quantitative, and functional aspects in the facial nerve regeneration process. MATERIALS AND METHODS: Forty-two male Wistar rats were used, randomly divided into a control group (CG; n = 10), in which the facial nerve without lesion was collected, and four experimental groups: (1) suture experimental group (SEG) and (2) fibrin experimental group (FEG), consisting of 16 animals in which the buccal branch of the facial nerve was sectioned on both sides of the face; an end-to-end epineural suture was performed on the right side, and a fibrin sealant was used on the left side for coaptation of the stumps; and (3) laser suture experimental group (LSEG) and (4) laser fibrin experimental group (LFEG), consisting of 16 animals that underwent the same surgical procedures as SEG and FEG with the addition of laser application at three different points along the surgical site (pulsed laser of 830 nm wavelength, optical output power of 30 mW, power density of 0...
May 26, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28552515/the-rabbit-as-a-behavioral-model-system-for-magnetic-resonance-imaging
#17
Craig Weiss, Daniel Procissi, John M Power, John F Disterhoft
BACKGROUND: fMRI requires that subjects not move during image acquisition. This has been achieved by instructing people not to move, or by anesthetizing experimental animal subjects to induce immobility. We have demonstrated that a surgically implanted headbolt onto the skull of a rabbit allows their brain to be imaged comfortably while the animal is awake. This article provides a detailed method for the preparation. NEW METHOD: We took advantage of the rabbit's tolerance for restraint to image the brain while holding the head at the standard stereotaxic angle...
May 25, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28549561/lateral-thalamic-control-of-nociceptive-response-after-whisker-pad-injection-of-varicella-zoster-virus
#18
Phillip R Kramer, Crystal Stinson, Mikhail Umorin, Mohong Deng, Mahesh Rao, Larry L Bellinger, Michael B Yee, Paul R Kinchington
Pain is a common complication of herpes zoster (HZ) infection which results from reactivation of a latent varicella zoster virus (VZV). A third of HZ patients' progress to a chronic pain state known as post herpetic neuralgia (PHN), and about a quarter of these patients' have orofacial pain. The mechanisms controlling the pain responses are not understood. Studies suggest central pathways involving the thalamus could control pain related to HZ, and studies in our lab suggest (VGAT) in the lateral thalamus influences orofacial pain...
May 24, 2017: Neuroscience
https://www.readbyqxmd.com/read/28546281/neurons-within-the-trigeminal-mesencephalic-nucleus-encode-for-the-kinematic-parameters-of-the-whisker-pad-macrovibrissae
#19
Ombretta Mameli, Marcello A Caria, Francesca Biagi, Marco Zedda, Vittorio Farina
It has been recently shown in rats that spontaneous movements of whisker pad macrovibrissae elicited evoked responses in the trigeminal mesencephalic nucleus (Me5). In the present study, electrophysiological and neuroanatomical experiments were performed in anesthetized rats to evaluate whether, besides the whisker displacement per se, the Me5 neurons are also involved in encoding the kinematic properties of macrovibrissae movements, and also whether, as reported for the trigeminal ganglion, even within the Me5 nucleus exists a neuroanatomical representation of the whisker pad macrovibrissae...
May 2017: Physiological Reports
https://www.readbyqxmd.com/read/28528964/inhibitory-interneurons-and-their-circuit-motifs-in-the-many-layers-of-the-barrel-cortex
#20
Dirk Feldmeyer, Guanxiao Qi, Vishalini Emmenegger, Jochen F Staiger
Recent years have seen substantial progress in studying the structural and function properties of GABAergic interneurons and their roles in the neuronal networks of barrel cortex. Although GABAergic interneurons represent only about 12% of the total number of neocortical neurons, they are extremely diverse with respect to their structural and functional properties. It has become clear that barrel cortex interneurons not only serve the maintenance of an appropriate excitation/inhibition balance but also are directly involved in sensory processing...
May 18, 2017: Neuroscience
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