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A V Ivanov, A G Dedul, Y N Fedotov, E V Komlichenko
BACKGROUND: At present, the patient preparation for IVF needs to undergo a series of planned tests, including the genotyping of single nucleotide polymorphism (SNP) alleles of some genes. In former USSR countries, such investigation was not included in overwhelming majority of health insurance programs and paid by patient. In common, there are prerequisites to the study of more than 50 polymorphisms. An important faced task is to determine the optimal panel for SNP genotyping in terms of price/number of SNP...
October 2016: Gynecological Endocrinology
Fang Yang, Hong Yang, Aramandla Ramesh, J Shawn Goodwin, Emmanuel U Okoro, ZhongMao Guo
UNLABELLED: We previously reported that overexpression of catalase upregulated xenobiotic- metabolizing enzyme (XME) expression and diminished benzo(a)pyrene (BaP) intermediate accumulation in mouse aortic endothelial cells (MAECs). Endoplasmic reticulum (ER) is the most active organelle involved in BaP metabolism. To examine the involvement of ER in catalase-induced BaP detoxification, we compared the level and distribution of XMEs, and the profile of BaP intermediates in the microsomes of wild-type and catalase transgenic endothelial cells...
2016: PloS One
Richard Ramsden, Evan P Gallagher
The transcription factor NFE2L2 (Nuclear Factor, Erythroid 2-Like 2, or NRF2) plays a key role in maintaining the redox state within cells. Characterization of this pathway has extended to fish, most notably zebrafish (Danio rerio), in which two paralogs of the transcription factor exist: Nrf2a, an activator, and Nrf2b, a negative regulator during embryogenesis. Only one ARE target has been thoroughly delineated in zebrafish, and this deviated from the canonical sequence derived from studies in mammals. In general, the mechanistic pathway has not been characterized in non-model aquatic organisms that are commonly exposed to environmental pollutants...
July 6, 2016: Redox Biology
David H McMillan, Jos L J van der Velden, Karolyn G Lahue, Xi Qian, Robert W Schneider, Martina S Iberg, James D Nolin, Sarah Abdalla, Dylan T Casey, Kenneth D Tew, Danyelle M Townsend, Colin J Henderson, C Roland Wolf, Kelly J Butnor, Douglas J Taatjes, Ralph C Budd, Charles G Irvin, Albert van der Vliet, Stevenson Flemer, Vikas Anathy, Yvonne M W Janssen-Heininger
Idiopathic pulmonary fibrosis (IPF) is a debilitating lung disease characterized by excessive collagen production and fibrogenesis. Apoptosis in lung epithelial cells is critical in IPF pathogenesis, as heightened loss of these cells promotes fibroblast activation and remodeling. Changes in glutathione redox status have been reported in IPF patients. S-glutathionylation, the conjugation of glutathione to reactive cysteines, is catalyzed in part by glutathione-S-transferase π (GSTP). To date, no published information exists linking GSTP and IPF to our knowledge...
June 2, 2016: JCI Insight
Olivia J Osborne, Katsuki Mukaigasa, Hitomi Nakajima, Bjorn Stolpe, Isabella Romer, Uzoma Philips, Iseult Lynch, Sulayman Mourabit, Shigehisa Hirose, Jamie R Lead, Makoto Kobayashi, Tetsuhiro Kudoh, Charles R Tyler
Some nanoparticles (NPs) may induce adverse health effects in exposed organisms, but to date the evidence for this in wildlife is very limited. Silver nanoparticles (AgNPs) can be toxic to aquatic organisms, including fish, at concentrations relevant for some environmental exposures. We applied whole mount in-situ hybridisation (WISH) in zebrafish embryos and larvae for a suite of genes involved with detoxifying processes and oxidative stress, including metallothionein (mt2), glutathionine S-transferase pi (gstp), glutathionine S-transferase mu (gstm1), haem oxygenase (hmox1) and ferritin heavy chain 1 (fth1) to identify potential target tissues and effect mechanisms of AgNPs compared with a bulk counterpart and ionic silver (AgNO3)...
November 2016: Nanotoxicology
Shanthi Ganesan, Aileen F Keating
Bisphenol A (BPA) is an endocrine disrupting chemical with ubiquitous human exposure. BPA causes primordial follicle loss and DNA damage in germ cells, thus we hypothesized that BPA induces ovarian DNA damage, thereby precipitating follicle loss. We also anticipated that the ovary activates DNA repair and xenobiotic biotransformation to minimize oocyte damage and/or, activate cell death signaling to deplete follicles. Postnatal day 4 F344 rat ovaries were cultured in medium containing vehicle control (1% dimethylsulfoxide [DMSO]) ± BPA (440 µM) for 2-8 days...
July 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
Jane T Jones, Xi Qian, Jos L J van der Velden, Shi Biao Chia, David H McMillan, Stevenson Flemer, Sidra M Hoffman, Karolyn G Lahue, Robert W Schneider, James D Nolin, Vikas Anathy, Albert van der Vliet, Danyelle M Townsend, Kenneth D Tew, Yvonne M W Janssen-Heininger
Nuclear Factor kappa B (NF-κB) is a transcription factor family critical in the activation of pro- inflammatory responses. The NF-κB pathway is regulated by oxidant-induced post-translational modifications. Protein S-glutathionylation, or the conjugation of the antioxidant molecule, glutathione to reactive cysteines inhibits the activity of inhibitory kappa B kinase beta (IKKβ), among other NF-κB proteins. Glutathione S-transferase Pi (GSTP) is an enzyme that has been shown to catalyze protein S-glutathionylation (PSSG) under conditions of oxidative stress...
August 2016: Redox Biology
Aml S Nasr, Rania M Sami, Noha Y Ibrahim, Dalia O Darwish
BACKGROUND: The super family of glutathione S-transferases (GSTs) is composed of multiple isoenzymes with significant evidence of functional polymorphic variation. GSTs detoxify potentially mutagenic and toxic DNA-reactive electrophiles, including metabolites of several chemotherapeutic agents, some of which are suspected human carcinogens. Polymorphisms within the phase II metabolizer enzymes GST T1, GST M1, and GST P1 affect the body's ability to detoxify a range of potential leukemogens encountered in the environment...
October 2015: Indian Journal of Cancer
Christian Kropf, Helmut Segner, Karl Fent
Embryos of oviparous fish, in contrast to (ovo) viviparous species, develop in the aquatic environment, and therefore need solute transport systems at their body surfaces for maintaining internal homeostasis and defending against potentially harmful substances. We hypothesized that solute transporters undergo changes in tissue distribution from the embryo to the larval stage. We therefore studied the mRNA profiles of eight ABC transporters (abcb1a, abcb1b, abcc1, abcc2, abcc3, abcc4, abcc5, abcg2) and three solute carriers (oatp1d, putative oatp2 putative, mate1) in different body regions (head, yolk sac epithelium, abdominal viscera, skin/muscles) of developing rainbow trout...
July 2016: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
Desirée Bartolini, Francesco Galli
Glutathione S-transferase P (GSTP), and possibly other members of the subfamily of cytosolic GSTs, are increasingly proposed to have roles far beyond the classical GSH-dependent enzymatic detoxification of electrophilic metabolites and xenobiotics. Emerging evidence suggests that these are essential components of the redox sensing and signaling platform of cells. GSTP monomers physically interact with cellular proteins, such as other cytosolic GSTs, signaling kinases and the membrane peroxidase peroxiredoxin 6...
April 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Zhiwei Ye, Jie Zhang, Tiffany Ancrum, Yefim Manevich, D M Townsend, Kenneth D Tew
S-glutathionylation of cysteine residues, catalyzed by glutathione S-transferase Pi (GSTP) alters structure/function characteristics of targeted proteins. We identify GSTP as an endoplasmic reticulum (ER)-resident protein where it demonstrates both chaperone and catalytic functions. Redox based proteomics identified a cluster of proteins cooperatively involved in regulation of ER stress (immunoglobulin heavy chain-binding protein [BiP], protein disulfide isomerase [PDI], calnexin, calreticulin, endoplasmin, sarco/endoplasmic reticulum Ca2+-ATPase [SERCA]) that individually co-immunoprecipitated with GSTP (implying protein complex formation) and were subject to S-glutathionylation...
February 3, 2016: Antioxidants & Redox Signaling
Meiping Tian, Benhua Zhao, Jie Zhang, Francis L Martin, Qingyu Huang, Liangpo Liu, Heqing Shen
Epidemiological studies implicate environmental risk factors and epigenetic alterations in the multistage process of hepatocellular carcinoma (HCC) development. However, associations between environmental factors and DNA methylation of tumour suppressor genes (TSGs) in HCC development remain ambiguous. Understanding how possible interactions influence risk may provide insights into the complexity of hepato-carcinogenesis. For this study, blood samples were collected from HCC patients (n=90) and healthy volunteers (n=99) from Xiamen (China) and data for selected environmental risk factors [e...
January 15, 2016: Science of the Total Environment
Renata Zelic, Valentina Fiano, Daniela Zugna, Chiara Grasso, Luisa Delsedime, Lorenzo Daniele, Diego Galliano, Andreas Pettersson, Anna Gillio-Tos, Franco Merletti, Lorenzo Richiardi
PURPOSE: Men at risk of missed prostate cancer on a negative biopsy often undergo a rebiopsy. We evaluated whether global hypomethylation, measured through LINE-1 methylation, and GSTP1 hypermethylation on a negative biopsy are associated with subsequent prostate cancer diagnosis. EXPERIMENTAL DESIGN: We performed a case-control study nested in an unselected series of 737 men who received at least two prostate biopsies at least three months apart at the Molinette Hospital (Turin, Italy)...
February 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Hanna Szaefer, Violetta Krajka-Kuźniak, Barbara Licznerska, Agnieszka Bartoszek, Wanda Baer-Dubowska
Our previous studies showed the diversified effect of cabbage juices and indoles on the estrogen metabolism key enzymes (CYP1A1, CYP1A2, CYP1B1) in breast epithelial cells differing in ER status, i.e., in tumorigenic-MCF7, MDA-MB-231 and non-tumorigenic-MCF10A cells. The aim of the present study was to further investigate the mechanism of chemopreventive action of cabbage juice and its active components by evaluating their effect on the expression of AhR, ERα, and Nrf2 using the same treatment regimen. The mRNA level of AhR and ERα was changed in a cell type-dependent manner and in general correlated with previously observed modulation of CYP expression...
2015: Nutrition and Cancer
Song I Yang, Byoung Ju Kim, So Yeon Lee, Hyo Bin Kim, Cheol Min Lee, Jinho Yu, Mi Jin Kang, Ho Sung Yu, Eun Lee, Young Ho Jung, Hyung Young Kim, Ju Hee Seo, Ji Won Kwon, Dae Jin Song, Gwangcheon Jang, Woo Kyung Kim, Jung Yeon Shim, Soo Young Lee, Hyeon Jong Yang, Dong In Suh, Seo Ah Hong, Kil Yong Choi, Youn Ho Shin, Kangmo Ahn, Kyung Won Kim, Eun Jin Kim, Soo Jong Hong
PURPOSE: To investigate whether prenatal exposure to indoor fine particulate matter (PM₂.₅) and environmental tobacco smoke (ETS) affects susceptibility to respiratory tract infections (RTIs) in infancy, to compare their effects between prenatal and postnatal exposure, and to determine whether genetic factors modify these environmental effects. METHODS: The study population consisted of 307 birth cohort infants. A diagnosis of RTIs was based on parental report of a physician's diagnosis...
November 2015: Allergy, Asthma & Immunology Research
David J McGarry, Probir Chakravarty, C Roland Wolf, Colin J Henderson
Acetaminophen (APAP) is the most commonly used over-the-counter analgesic. However, hepatotoxicity induced by APAP is a major clinical issue, and the factors that define sensitivity to APAP remain unclear. We have previously demonstrated that mice nulled for glutathione S-transferase Pi (GSTP) are resistant to APAP-induced hepatotoxicity. This study aims to exploit this difference to delineate pathways of importance in APAP toxicity. We used mice nulled for GSTP and heme oxygenase-1 oxidative stress reporter mice, together with a novel nanoflow liquid chromatography-tandem mass spectrometry methodology to investigate the role of oxidative stress, cell signaling, and protein S-glutathionylation in APAP hepatotoxicity...
November 2015: Journal of Pharmacology and Experimental Therapeutics
S V Rana, J K Kamboj, S K Sharma, R P Ola, S K Sinha, K Singh
INTRODUCTION: Hepatotoxicity is a serious adverse effect of antituberculosis treatment (ATT). Glutathione S-transferase (GST) is involved in the detoxification of toxic metabolites produced as a result of ATT, increased oxidative stress and decreased antioxidant levels, and differences in the GST polymorphism may be one of the causes of ATT-induced hepatotoxicity. AIM: This study was undertaken to study the relationship among antioxidant status, oxidative stress and GST gene polymorphisms in the development of ATT-induced hepatotoxicity in Indian patients...
July 2013: Hepatology International
Daniel J Conklin, Yiru Guo, Ganapathy Jagatheesan, Peter J Kilfoil, Petra Haberzettl, Bradford G Hill, Shahid P Baba, Luping Guo, Karin Wetzelberger, Detlef Obal, D Gregg Rokosh, Russell A Prough, Sumanth D Prabhu, Murugesan Velayutham, Jay L Zweier, J David Hoetker, Daniel W Riggs, Sanjay Srivastava, Roberto Bolli, Aruni Bhatnagar
RATIONALE: Myocardial ischemia-reperfusion (I/R) results in the generation of oxygen-derived free radicals and the accumulation of lipid peroxidation-derived unsaturated aldehydes. However, the contribution of aldehydes to myocardial I/R injury has not been assessed. OBJECTIVE: We tested the hypothesis that removal of aldehydes by glutathione S-transferase P (GSTP) diminishes I/R injury. METHODS AND RESULTS: In adult male C57BL/6 mouse hearts, Gstp1/2 was the most abundant GST transcript followed by Gsta4 and Gstm4...
August 14, 2015: Circulation Research
D Bartolini, J Commodi, M Piroddi, L Incipini, L Sancineto, C Santi, F Galli
Glutathione S-transferase pi (GSTP), a phase II gene downstream of the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant-responsive element (ARE)/electrophile response element (EpRE) transcription pathway, plays a key role in both the signaling and detoxification response to Se-organic compounds with thiol peroxidase activity. We here investigated the role of GSTP on the Nrf2 activation response of cells challenged with a new class of diselenides derived from the basic structure of diphenyl diselenide [(PhSe)2]...
November 2015: Free Radical Biology & Medicine
Wei-Jun Sun, Yun-Wei Pang, Yan Liu, Hai-Sheng Hao, Xue-Ming Zhao, Tong Qin, Hua-Bin Zhu, Wei-Hua Du
To determine the beneficial effects and mechanisms of exogenous glutathione (GSH) during IVC on the embryonic development, bovine IVF zygotes were cultured in medium containing different concentrations of GSH, and the rate of cleavage and blastocyst development, total cell number of blastocysts, the inner cell mass:total cell number ratio, and intracellular GSH and reactive oxygen species concentrations were investigated. Gene expressions associated with embryonic development and GSH metabolism were measured using quantitative real-time polymerase chain reaction...
September 15, 2015: Theriogenology
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