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https://www.readbyqxmd.com/read/29131417/culturing-with-modified-egm2-medium-enhances-porcine-neonatal-islet-like-cell-clusters-resistance-to-apoptosis-in-islet-xenotransplantation
#1
Xiaoqian Ma, Cejun Yang, Juan Zhang, Jia Wang, Wei Li, Chang Xu, Pengfei Rong, Bin Ye, Minghua Wu, Jianhui Jiang, Shounan Yi, Wei Wang
BACKGROUND: Neonatal pig islet-like cell clusters (NICC) are an attractive source of insulin-producing tissue for potential transplantation treatment of type 1 diabetic patients. However, a considerable loss of NICC after their transplantation due to apoptosis resulted from islet isolation and instant blood-mediated inflammatory reaction remains to be overcome. METHODS: EGM2 medium depleted with hydrocortisone and supplemented with 50 mmol/L isobutylmethylxanthine, 10 mmol/L nicotinamide, and 10 mmol/L glucose was used to culture NICC at day 1, the day after isolation and changed every other day...
November 12, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29127200/steroid-receptor-coactivator-2-src-2-coordinates-cardiomyocyte-paracrine-signaling-to-promote-pressure-overload-induced-angiogenesis
#2
Ji Ho Suh, Li Lai, Deokhwa Nam, Jong Kim, Juyeon Jo, George E Taffet, Eunah Kim, Jason T Kaelber, Hyun-Kyoung Lee, Mark L Entman, John P Cooke, Erin L Reineke
Pressure overload-induced cardiac stress induces left ventricular hypertrophy driven by increased cardiomyocyte mass. The increased energetic demand and cardiomyocyte size during hypertrophy necessitate increased fuel and oxygen delivery and stimulate angiogenesis in the left ventricular wall. We have previously shown that the transcriptional regulator steroid receptor coactivator-2 (SRC-2) controls activation of several key cardiac transcription factors and that SRC-2 loss results in extensive cardiac transcriptional remodeling...
November 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29125632/median-sternotomy-versus-intercostal-thoracotomy-for-lung-lobectomy-a-comparison-of-short-term-outcome-in-134-dogs
#3
Seth Bleakley, Kevin Phipps, Brian Petrovsky, Eric Monnet
OBJECTIVE: To compare short-term outcomes between dogs undergoing lung lobectomy via median sternotomy (MS) or intercostal thoracotomy (ICT). STUDY DESIGN: Retrospective case control study. METHODS: Medical records of dogs that underwent lung lobectomy via MS or ICT at a single institution were reviewed for demographics, intraoperative findings, and postoperative management and outcomes. Dogs with pleural effusion, pneumonia, migrating foreign body, lung abscess, spontaneous pneumothorax, or lung lobe torsion were excluded...
November 10, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29119549/a-prototype-small-bore-ventilation-catheter-with-a-cuff-cuff-inflation-optimizes-ventilation-with-the-ventrain
#4
M W P de Wolf, T van der Beek, A E Hamaekers, M Theunissen, D Enk
BACKGROUND: Ventilation through small-diameter tubes typically precludes use of a cuff as this will impede the necessary passive outflow of gas alongside the tube's outer surface. Ventrain assists expiration and enables oxygenation and normoventilation through small-bore cannulas or catheters, particularly in obstructed airways. A small-bore ventilation catheter (SBVC; 40 cm long, 2.2 mm inner diameter) with a separate pressure monitoring lumen and a cuff was developed. Efficacy of oxygenation and ventilation with Ventrain through this catheter was investigated in sealed and open airways in a porcine cross-over study...
November 8, 2017: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/29117076/tissue-oxygenation-and-negative-pressure-wound-therapy-when-applied-to-the-feet-of-persons-with-diabetes-mellitus-an-observational-study
#5
Ye-Na Lee, Jong Seok Lee, Seung-Kyu Han, Hye-Kyung Jung
PURPOSE: Our group has reported that negative-pressure wound therapy (NPWT) decreases tissue oxygenation by 84% in the foot of diabetic patients because the pad of the connecting drainage tube and foam sponge of the NPWT system compress the wound bed. The purpose of this study was to determine whether an NPWT modified dressing application reduces tissue oxygenation in the feet of persons with diabetes mellitus. DESIGN: A prospective, clinical, observational study...
November 2017: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/29109835/anterior-submandibular-approach-for-transmylohyoid-endotracheal-intubation-a-reappraisal-with-prospective-study-in-206-cases-of-craniomaxillofacial-fractures
#6
Nitin Bhola, Anendd Jadhav, Atul Kala, Rahul Deshmukh, Umesh Bhutekar, G S V Prasad
Despite a paradigm shift in anesthesia and trauma airway management, the craniomaxillofacial fracture (CMF) patients continue to pose a challenge. A prospective study was planned between April 2007 and March 2015 to investigate the safety, efficacy, utility, and complications of anterior submandibular approach for transmylohyoid intubation (TMI) in CMFs using an armored endotracheal tube (ETT). Out of 1,207 maxillofacial trauma cases reported, this study recruited 206 patients (152 males and 54 females) aged between 21 and 60 years...
December 2017: Craniomaxillofacial Trauma & Reconstruction
https://www.readbyqxmd.com/read/29104338/a-new-bomb-combustion-system-for-tritium-extraction
#7
Richard I Marsh, Ian W Croudace, Phillip E Warwick, Natasha Cooper, Nadereh St-Amant
Quantitative extraction of tritium from a sample matrix is critical to efficient measurement of the low-energy pure beta emitter. Oxidative pyrolysis using a tube furnace (Pyrolyser) has been adopted as an industry standard approach for the liberation of tritium (Warwick et al. in Anal Chim Acta 676:93-102, 2010) however pyrolysis of organic-rich materials can be problematic. Practically, the mass of organic rich sample combusted is typically limited to <1 g to minimise the possibility of incomplete combustion...
2017: Journal of Radioanalytical and Nuclear Chemistry
https://www.readbyqxmd.com/read/29100869/oxidative-stress-induced-mir-27a-targets-the-redox-gene-nrf2-in-diabetic-embryopathy
#8
Yang Zhao, Daoyin Dong, E Albert Reece, Ashley R Wang, Peixin Yang
BACKGROUND: Maternal diabetes induces neural tube defects (NTDs), and oxidative stress is a causal factor for maternal diabetes-induces NTDs. The redox gene Nrf2 (nuclear factor-erythroid 2-related factor 2) is the master regulator of the cellular antioxidant system. OBJECTIVE: In the present study, we aimed to determine whether maternal diabetes inhibits Nrf2 expression and Nrf2-controlled antioxidant genes through the redox-sensitive miR-27a. STUDY DESIGN: We used a well-established type 1 diabetic embryopathy mouse model induced by streptozotocin for our in vivo studies...
October 31, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29100366/vegf-stimulated-the-angiogenesis-by-promoting-the-mitochondrial-functions
#9
Dongqing Guo, Qiyan Wang, Chun Li, Yong Wang, Xing Chen
The vascular endothelial growth factor (VEGF) signaling pathway involved in angiogenesis which plays a pivotal role in normal development and also represents a major therapeutic target for tumors and intraocular neovascular disorders. The aims of the present study were to evaluate the effects of VEGF on endothelial cells and clarify the mechanism. Here, we showed that VEGF significantly stimulated the proliferation, migration and cell cycle of endothelial cells, and it also induced tube formation in vitro significantly...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29090012/infection-of-a-tomato-cell-culture-by-phytophthora-infestans-a-versatile-tool-to-study-phytophthora-host-interactions
#10
Charikleia Schoina, Klaas Bouwmeester, Francine Govers
BACKGROUND: The oomycete Phytophthora infestans causes late blight on potato and tomato. Despite extensive research, the P. infestans-host interaction is still poorly understood. To find new ways to further unravel this interaction we established a new infection system using MsK8 tomato cells. These cells grow in suspension and can be maintained as a stable cell line that is representative for tomato. RESULTS: MsK8 cells can host several Phytophthora species pathogenic on tomato...
2017: Plant Methods
https://www.readbyqxmd.com/read/29077897/in-vivo-oxygen-temperature-and-ph-dynamics-in-the-female-reproductive-tract-and-their-importance-in-human-conception-a-systematic-review
#11
Ka Ying Bonnie Ng, Roel Mingels, Hywel Morgan, Nick Macklon, Ying Cheong
BACKGROUND: Despite advances in ART, implantation and pregnancy rates per embryo transfer still remain low. IVF laboratories strive to ensure that the process of handling gametes in vitro closely mimics the in vivo environment. However, there remains a lack of knowledge regarding the in vivo regulation and dynamic variation in biophysical parameters such as oxygen concentration, pH and temperature within the reproductive tract. OBJECTIVE AND RATIONALE: To undertake a systematic review of the current understanding of the physico-chemical parameters of oxygen tension (pO2), pH and temperature within the female reproductive tract, and their potential implications in clinical and pathological processes related to fertility and those pertaining to limited reproductive capacity...
October 25, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29071770/the-child-s-behavior-during-inhalational-induction-and-its-impact-on-the-anesthesiologist-s-sevoflurane-exposure
#12
Jennifer Herzog-Niescery, Heike Vogelsang, Martin Bellgardt, Nikolaj Matthias Botteck, Hans-Martin Seipp, Horst Bartz, Thomas Peter Weber, Philipp Gude
BACKGROUND: Sevoflurane is commonly used for inhalational inductions in children, but the personnel's exposure to it is potentially harmful. Guidance to reduce gas pollution refers mainly to technical aspects, but the impact of the child's behavior has not yet been studied. AIMS: The purpose of this study was to determine how child behavior, according to the Frankl Behavioral Scale, affects the amount of waste sevoflurane in anesthesiologists' breathing zones. METHODS: Sixty-eight children aged 36-96 months undergoing elective ENT surgery were recruited for this prospective, observational investigation...
December 2017: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/29065402/vitamin-e-coated-dialyzer-inhibits-oxidative-stress
#13
Shiho Yamadera, Yuya Nakamura, Masahiro Inagaki, Isao Ohsawa, Hiromichi Gotoh, Yoshikazu Goto, Naoki Sato, Tatsunori Oguchi, Yurika Gomi, Mayumi Tsuji, Yuji Kiuchi, Shinichi Iwai
AIM: To examine the effects of vitamin E-coated dialyzer on oxidative stress in vitro. METHODS: A dialyzer with a synthetic polymer membrane (APS-11SA) and vitamin E-coated dialyzer (VPS-11SA) were connected to a blood tubing line, and U937 cells were circulated in the device. The circulating fluid was collected at 1, 2, 5, 10, 25, and 50 cycles, which are estimated numbers of passes through the dialyzer. Intracellular reactive oxygen species (ROS) production, malondialdehyde (MDA), and Cu/Zn-superoxide dismutase (SOD) were quantified...
October 25, 2017: Blood Purification
https://www.readbyqxmd.com/read/29063722/a-quality-improvement-initiative-to-increase-the-safety-of-pediatric-emergency-airway-management
#14
Elliot Long, Domenic R Cincotta, Joanne Grindlay, Stefano Sabato, Emmanuelle Fauteux-Lamarre, David Beckerman, Terry Carroll, Nuala Quinn
BACKGROUND: Emergency airway management is commonly associated with life-threatening hypoxia and hypotension which may be preventable. AIMS: The aim of this quality improvement study was to reduce the frequency of intubation-related hypoxia and hypotension. METHODS: This prospective quality improvement study was conducted over 4 years in the Emergency Department of The Royal Children's Hospital, Melbourne, Australia. A preintervention cohort highlighted safety gaps and was used to design study interventions, including an emergency airway algorithm, standardized airway equipment, a preintubation checklist and equipment template, endtidal carbon dioxide monitoring, postintubation team debriefing, and multidisciplinary team training...
October 24, 2017: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/29057926/engraftment-and-morphological-development-of-vascularized-human-ips-cell-derived-3d-cardiomyocyte-tissue-after-xenotransplantation
#15
Hirokazu Narita, Fumiaki Shima, Junya Yokoyama, Shigeru Miyagawa, Yoshinari Tsukamoto, Yasushi Takamura, Ayami Hiura, Ken Fukumoto, Tomohiro Chiba, Seiji Watanabe, Yoshiki Sawa, Mitsuru Akashi, Hiroshi Shimoda
One of the major challenges in cell-based cardiac regenerative medicine is the in vitro construction of three-dimensional (3D) tissues consisting of induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) and a blood vascular network supplying nutrients and oxygen throughout the tissue after implantation. We have successfully built a vascularized iPSC-CM 3D-tissue using our validated cell manipulation technique. In order to evaluate an availability of the 3D-tissue as a biomaterial, functional morphology of the tissues was examined by light and transmission electron microscopy through their implantation into the rat infarcted heart...
October 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29056498/a-comparison-of-3-videolaryngoscopes-for-double-lumen-tube-intubation-in-humans-by-users-with-mixed-experience-a-randomized-controlled-study
#16
Mohamed R El-Tahan, Alaa M Khidr, Ihab S Gaarour, Saeed A Alshadwi, Talal M Alghamdi, Abdulmohsen Al'ghamdi
OBJECTIVES: To test the hypothesis that laryngoscopy using the Airtraq (Prodol Limited, Viscaya, Spain) or King Vision laryngoscope (KVL) (Ambu A/S, Ballerup, Denmark) would result in a shorter time for successful double-lumen endobronchial tube (DLT) intubation by users with mixed experience than the time required using the Macintosh or GlideScope (Verathon Inc., Bothell, WA) laryngoscopes. DESIGN: A randomized, prospective, blind study. SETTING: A single university hospital...
August 4, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/29054767/identifying-eustachian-tube-dysfunction-prior-to-hyperbaric-oxygen-therapy-who-is-at-risk-for-intolerance
#17
Jason E Cohn, Michael Pfeiffer, Niki Patel, Robert T Sataloff, Brian J McKinnon
PURPOSE: Determine whether specific risk factors, symptoms and clinical examination findings are associated with hyperbaric oxygen therapy (HBOT) intolerance and subsequent tympanotomy tube placement. MATERIALS AND METHODS: A retrospective case series with chart review was conducted from 2007 to 2016 of patients undergoing HBOT clearance at a tertiary care university hospital in an urban city. Eighty-one (n=81) patient charts were reviewed for risk factors, symptoms and clinical examination findings related to HBOT eustachian tube dysfunction and middle ear barotrauma...
October 10, 2017: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/29050901/medical-device-related-pressure-injuries-an-exploratory-descriptive-study-in-an-acute-tertiary-hospital-in-australia
#18
Michelle Barakat-Johnson, Catherine Barnett, Timothy Wand, Kathryn White
AIM: To examine and explore medical device-related pressure injuries in an 800-bed tertiary hospital. MATERIALS AND METHODS: An exploratory descriptive study design was employed. A prospective review of all data on reported hospital-acquired pressure injuries was conducted on a weekly basis from July 2015 to August 2016. This included a patient assessment and medical record review as well as brief semi-structured interviews with nurses. RESULTS: The overall incidence of medical device-related pressure injuries was 27...
October 4, 2017: Journal of Tissue Viability
https://www.readbyqxmd.com/read/29049710/the-effect-of-antegrade-pulmonary-blood-flow-following-a-late-bidirectional-glenn-procedure
#19
Tao Yan, Guang Tong, Ben Zhang, Feng Yan, Xuan Zhou, Xianyue Wang, Hua Lu, Tao Ma, Xiaowu Wang, Hao Yu, Zhongchan Sun, Weida Zhang
OBJECTIVES: The effect of antegrade pulmonary blood flow (APBF) has never been studied in the bidirectional Glenn (BDG) procedure performed late. METHODS: Records of 112 consecutive patients who had a BDG procedure during a 10-year period were reviewed retrospectively. The patients were divided into 2 groups based on whether APBF occurred following the BDG procedure (APBF group, n = 81) or not (non-APBF group, n = 31). The median age at the BDG procedure was 6...
October 13, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29047763/passive-ranging-using-a-filter-based-non-imaging-method-based-on-oxygen-absorption
#20
Hao Yu, Bingqi Liu, Zongqun Yan, Yu Zhang
To solve the problem of poor real-time measurement caused by a hyperspectral imaging system and to simplify the design in passive ranging technology based on oxygen absorption spectrum, a filter-based non-imaging ranging method is proposed. In this method, three bandpass filters are used to obtain the source radiation intensities that are located in the oxygen absorption band near 762 nm and the band's left and right non-absorption shoulders, and a photomultiplier tube is used as the non-imaging sensor of the passive ranging system...
October 1, 2017: Applied Optics
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