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https://www.readbyqxmd.com/read/28701710/deficient-vitamin-e-uptake-during-development-impairs-neural-tube-closure-in-mice-lacking-lipoprotein-receptor-sr-bi
#1
Nicolás Santander, Carlos Lizama, María José Parga, Alonso Quiroz, Druso Pérez, Guadalupe Echeverría, Lorena Ulloa, Verónica Palma, Attilio Rigotti, Dolores Busso
SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI(-/-) embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic vitamin E uptake during murine neural tube closure...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28700565/nasal-continuous-positive-airway-pressure-influences-bottle-feeding-in-preterm-lambs
#2
Nathalie Samson, Audrey Michaud, Rahmeh Othman, Charlène Nadeau, Stéphanie Nault, Danny Cantin, Michaël Sage, Céline Catelin, Jean-Paul Praud
BACKGROUND: In preterm infants, the time from initiation to full oral feeding can take weeks, which represents a very worrisome problem in neonatal medicine. Although current knowledge suggests that oral feeding should be introduced early, this is often delayed due to the need for prolonged nasal continuous positive airway pressure (nCPAP). Indeed, most caregivers fear that nCPAP could disrupt sucking-swallowing-breathing coordination and induce tracheal aspiration. The goal of the present study was to assess the impact of nCPAP delivered by the Infant Flow System on the physiology of sucking-swallowing-breathing coordination during bottle-feeding in preterm lambs over 24 h...
July 12, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28686377/-airways-access-in-cannot-intubate-situation-cricothyrotomy
#3
Frédéric Heymans, Pavel Dulguerov
In case of impossible intubation /oxygenation because of an upper airways obstruction, a cricothyrotomy is indicated. It is a relative simple and safe procedure that may be life saving. A blade, a tube and one's index finger are the only tools needed. The procedure can be done anywhere and every physician should be able to perform it. Several devices have been developed to try to improve it's success rate ; on the contrary, they render the task more complicated and lead to double failure rates with associated lesions, as described in our comparative study...
October 5, 2016: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28677750/pm2-5-exposure-decreases-viability-migration-and-angiogenesis-in-human-umbilical-vein-endothelial-cells-and-human-microvascular-endothelial-cells
#4
Shengguang Chen, Xianzheng Wu, Jiawen Hu, Guoxing Dai, Aihong Rong, Gang Guo
Previous studies have confirmed that exposure to particulate matter with a diameter of ≤2.5 µm (PM2.5) is associated with inflammation. PM2.5 decreases cardiac cell viability and increases apoptosis through overproduction of reactive oxygen species (ROS). In the present study, the role of PM2.5 in ECs was investigated in vitro. Human umbilical vein endothelial cells and human microvascular endothelial cells (ECs) were incubated with PM2.5 (100‑800 µg/ml) to investigate the effects of PM2.5 on EC viability, migration, tube formation and intracellular levels of ROS...
June 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28673515/high-glucose-induced-p53-phosphorylation-contributes-to-impairment-of-endothelial-antioxidant-system
#5
Yong Wu, Sangkyu Lee, Selene Bobadilla, Sheng Zhong Duan, Xuan Liu
High levels of glucose (HG) induce reactive oxygen species-mediated oxidative stress in endothelial cells (ECs), which leads to endothelial dysfunction and tissue damage. However, the molecular mechanisms involved in HG-induced endothelial oxidative stress and damage remain elusive. Here we show that cellular ATP level-modulated p53 Thr55 phosphorylation plays a critical role in the process. Upon HG exposure, the elevated ATP levels induced the kinase activity of TAF1 (TBP-associated factor 1), which leads to p53 Thr55 phosphorylation...
June 30, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28666875/in%C3%A2-vitro-and-in%C3%A2-vivo-inhibition-of-proangiogenic-retinal-phenotype-by-an-antisense-oligonucleotide-downregulating-upar-expression
#6
Matteo Lulli, Maurizio Cammalleri, Irene Granucci, Ewa Witort, Silvia Bono, Federico Di Gesualdo, Antonella Lupia, Rosa Loffredo, Giovanni Casini, Massimo Dal Monte, Sergio Capaccioli
Neoangiogenesis is the main pathogenic event involved in a variety of retinal diseases. It has been recently demonstrated that inhibiting the urokinase-type plasminogen activator receptor (uPAR) results in reduced angiogenesis in a mouse model of oxygen-induced retinopathy (OIR), establishing uPAR as a therapeutic target in proliferative retinopathies. Here, we evaluated in cultured human retinal endothelial cells (HRECs) and in OIR mice the potential of a specific antisense oligodeoxyribonucleotide (ASO) in blocking the synthesis of uPAR and in providing antiangiogenic effects...
June 27, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28665311/antioxidant-activity-of-yichun-blue-honeysuckle-ybhs-berry-counteracts-ccl%C3%A2-induced-toxicity-in-liver-injury-model-of-mice
#7
Mian-Ying Wang, Madhuwanti Srinivasan, Subramanyam Dasari, Parnal Narvekar, Angela Lincy Prem Antony Samy, Venkata Satish Dontaraju, Lin Peng, Gary L Anderson, Gnanasekar Munirathinam
Yichun Blue Honeysuckle (YBHS) is reported to have a broad range of health benefits including protection against a number of chronic diseases. The objective of our study was to determine whether YBHS exhibits antioxidant activity, and if so, determine how it provides protection against oxidative stress. Eight-week old mice (25 male and 25 female) were randomized into five groups (n = 10 per group). YBHS extract (at 6.25%, 12.5%, or 25%) was administrated via intra-gastric tube to mice at 0.1 mL/10 g body weight once daily for 7 days...
June 30, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28661035/up-regulated-basigin-2-in-microglia-induced-by-hypoxia-promotes-retinal-angiogenesis
#8
Jie Yin, Wen-Qin Xu, Ming-Xiang Ye, Yong Zhang, Hai-Yan Wang, Jian Zhang, Yu Li, Yu-Sheng Wang
Retinal microglia cells contribute to vascular angiogenesis and vasculopathy induced by relative hypoxia. However, its concrete molecular mechanisms in shaping retinal angiogenesis have not been elucidated. Basigin, being involved in tumour neovasculogenesis, is explored to exert positive effects on retinal angiogenesis induced by microglia. Therefore, we set out to investigate the expression of basigin using a well-characterized mouse model of oxygen-induced retinopathy, which recapitulated hypoxia-induced aberrant neovessel growth...
June 29, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28655497/pharmacokinetics-of-dexmedetomidine-in-isoflurane-anesthetized-new-zealand-white-rabbits
#9
Ryan S Bailey, Linda S Barter, Bruno H Pypendop
OBJECTIVE: To characterize the pharmacokinetics of dexmedetomidine when administered as a short intravenous (IV) infusion to isoflurane-anesthetized rabbits. STUDY DESIGN: Experimental study. ANIMALS: A total of six healthy adult female New Zealand White rabbits. METHODS: Rabbits were anesthetized with isoflurane in oxygen. Following determination of isoflurane minimum alveolar concentration (MAC), the anesthetic dose was reduced to 0...
March 6, 2017: Veterinary Anaesthesia and Analgesia
https://www.readbyqxmd.com/read/28653420/impact-of-endotracheal-tube-shortening-on-work-of-breathing-in-neonatal-and-pediatric-in-vitro-lung-models
#10
Rebecca Mohr, Jörg Thomas, Vincenzo Cannizzaro, Markus Weiss, Alexander R Schmidt
BACKGROUND: Work of breathing accounts for a significant proportion of total oxygen consumption in neonates and infants. Endotracheal tube inner diameter and length significantly affect airflow resistance and thus work of breathing. While endotracheal tube shortening reduces endotracheal tube resistance, the impact on work of breathing in mechanically ventilated neonates and infants remains unknown. AIM: The objective of this in vitro study was to quantify the effect of endotracheal tube shortening on work of breathing in simulated pediatric lung settings...
June 27, 2017: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/28645964/the-predictive-value-of-serial-changes-in-diaphragm-function-during-the-spontaneous-breathing-trial-for-weaning-outcome-a-study-protocol
#11
Pengmin Zhou, Zhongheng Zhang, Yucai Hong, Huabo Cai, Hui Zhao, Peifeng Xu, Yiming Zhao, Shengping Lin, Xuchang Qin, JiaWei Guo, Yun Pan, Junru Dai
INTRODUCTION: There is a variety of tools being used in clinical practice for the prediction of weaning success from mechanical ventilation. However, their diagnostic performances are less than satisfactory. The purpose of this study is to investigate the value of serial changes in diaphragm function measured by ultrasound during the spontaneous breathing trial (SBT) as a weaning predictor. METHODS AND ANALYSIS: This is a prospective observational study conducted in a 10-bed medical emergency intensive care unit (EICU) in a university-affiliated hospital...
June 23, 2017: BMJ Open
https://www.readbyqxmd.com/read/28640104/the-easytube-during-general-anesthesia-for-minor-surgery-a-randomized-controlled-trial
#12
RANDOMIZED CONTROLLED TRIAL
Oliver Robak, Sonia Vaida, Luis Gaitini, Andreas Thierbach, Ricardo Urtubia, Peter Krafft, Michael Frass
BACKGROUND: The EasyTube (EzT) is a supraglottic airway device that is used for emergency airway situations. Ventilation during general anesthesia should also be feasible, but literature on the EzT is scarce. We evaluated the EzT in comparison with the endotracheal tube (ETT) in its use during general anesthesia in a comparative study. METHODS: A total of 400 patients with American Society of Anesthesiologists (ASA) physical status I to II scheduled for minor surgery in 4 centers were randomized for ventilation via the ETT or EzT...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28637844/a-dirty-cause-of-vancomycin-mediated-henoch-schonlein-purpura-oxygen-tubing-is-not-a-foley
#13
Nikhil H Shah, Kristopher P Kline, Manas K Shukla
A 59-year-old male presented with methicillin-resistantStaphylococcus aureus bacteraemia from a prostatic abscess and was treated with vancomycin. Two weeks into his treatment course, he developed severe joint pains, abdominal pain with bloody, mucinous stools and a diffuse palpable purpuric rash on his extremities. Biopsy of the rash showed IgA immune-complex deposition consistent with Henoch-Schönlein purpura. After treatment with glucocorticoids, his symptoms resolved completely. Vancomycin is an extremely commonly used antibiotic with certain well-known adverse effects...
June 20, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28630660/npc-exs-alleviate-endothelial-oxidative-stress-and-dysfunction-through-the-mir-210-downstream-nox2-and-vegfr2-pathways
#14
Hua Liu, Jinju Wang, Yusen Chen, Yanfang Chen, Xiaotang Ma, Ji C Bihl, Yi Yang
We have demonstrated that neural progenitor cells (NPCs) protect endothelial cells (ECs) from oxidative stress. Since exosomes (EXs) can convey the benefit of parent cells through their carried microRNAs (miRs) and miR-210 is ubiquitously expressed with versatile functions, we investigated the role of miR-210 in the effects of NPC-EXs on oxidative stress and dysfunction in ECs. NPCs were transfected with control and miR-210 scramble/inhibitor/mimic to generate NPC-EXs(con), NPC-EXs(sc), NPC-EXs(anti-miR-210), and NPC-EXs(miR-210)...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28621839/evaluation-of-hemodynamic-performance-of-a-combined-ecls-and-crrt-circuit-in-seven-positions-with-a-simulated-neonatal-patient
#15
Elizabeth Profeta, Kaitlyn Shank, Shigang Wang, Christian O'Connor, Allen R Kunselman, Karl Woitas, John L Myers, Akif Ündar
As it is common for patients treated with extracorporeal life support (ECLS) to subsequently require continuous renal replacement therapy (CRRT), and neonatal patients encounter limitations due to lack of access points, inclusion of CRRT in the ECLS circuit could provide advanced treatment for this population. The objective of this study was to evaluate an alternative neonatal ECLS circuit containing either a Maquet RotaFlow centrifugal pump or Maquet HL20 roller pump with one of seven configurations of CRRT using the Prismaflex 2000 System...
June 16, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28619111/two-minute-disconnection-technique-with-a-double-lumen-tube-to-speed-the-collapse-of-the-non-ventilated-lung-for-one-lung-ventilation-in-thoracoscopic-surgery
#16
Qiongzhen Li, Xiaofeng Zhang, Jingxiang Wu, Meiying Xu
BACKGROUND: Thoracic surgery requires the effective collapse of the non-ventilated lung. In the majority of cases, we accomplished, accelerated lung collapse using a double-lumen tube (DLT). We hypothesized that using the two-minute disconnection technique with a DLT would improve lung collapse during subsequent one-lung ventilation. METHODS: Fifty patients undergoing thoracoscopic surgery with physical classification I or II according to the American Society of Anesthesiologists were randomly divided into two groups for respiratory management of one-lung ventilation (OLV)...
June 15, 2017: BMC Anesthesiology
https://www.readbyqxmd.com/read/28615394/hyperbaric-oxygen-therapy-for-children-with-autism-spectrum-disorder
#17
Teeranai Sakulchit, Chris Ladish, Ran D Goldman
Question As autism spectrum disorder (ASD) is a multifactorial condition, with genetic and environmental risk factors contributing to children's unique presentation and symptom severity, a range of treatments have been suggested. Parents of children with ASD in my clinic are asking me about alternative therapies to improve their children's condition. One of those therapies is hyperbaric oxygen therapy (HBOT); commercial advertisement in the past has suggested good results with this approach. Should I recommend the use of HBOT for children with ASD? Answer Hyperbaric oxygen therapy provides a higher concentration of oxygen delivered in a chamber or tube containing higher than sea level atmospheric pressure...
June 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/28611757/the-protein-disulfide-isomerase-of-botrytis-cinerea-an-er-protein-involved-in-protein-folding-and-redox-homeostasis-influences-nadph-oxidase-signaling-processes
#18
Robert Marschall, Paul Tudzynski
Botrytis cinerea is a filamentous plant pathogen, which infects hundreds of plant species; within its lifestyle, the production of reactive oxygen species (ROS) and a balanced redox homeostasis are essential parameters. The pathogen is capable of coping with the plant's oxidative burst and even produces its own ROS to enhance the plant's oxidative burst. Highly conserved NADPH oxidase (Nox) complexes produce the reactive molecules. The membrane-associated complexes regulate a large variety of vegetative and pathogenic processes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28605977/linalool-induced-oxidative-stress-processes-in-the-human-pathogen-candida-albicans
#19
Gábor Máté, Dominika Kovács, Zoltán Gazdag, Miklós Pesti, Árpád Szántó
The present study investigated the linalool (Lol)-induced effects in acute toxicity tests in the human pathogen Candida albicans (C. albicans). Lol treatments induced reduced germ tube formation of the pathogen, which plays a crucial role in the virulence. In comparison with the untreated control, the exposure of 107 cells ml(-1) to 0.7 mM or 1.4 mM Lol for one hour induced 20% and 30% decrements, respectively, in the colony-forming ability. At the same time, these treatments caused dose-dependent decrease in the levels of superoxide anion radical and total reactive oxygen species, while there was 1...
June 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28604085/tension-pneumothorax-after-percutaneous-tracheostomy
#20
Sarina Matsumura, Naotaka Kishimoto, Tomio Iseki, Yoshihiro Momota
A 76-year-old woman with right mandibular gingival cancer was scheduled for surgery. A percutaneous tracheostomy kit was used for tracheostomy under intravenous sedation. After puncturing the cricothyroid membrane, a dilator was inserted along a guidewire. Bucking was observed at the time of insertion of the dilator, despite intratracheal lidocaine spray applied before insertion. After that, the tracheostomy tube was inserted, but no capnographic waveforms appeared when the tube was connected to the anesthesia circuit...
2017: Anesthesia Progress
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