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Ahmed B Hamid Al-Rikabi, Sona Pekova, Xioabo Fan, Tereza Jančušková, Thomas Liehr
Background: Cytogenetically visible chromosomal imbalances in humans are deleterious and adverse in the majority of the cases. However, healthy persons living with chromosomal imbalances in the range of several megabasepairs (Mbps) in size, like carriers of small Supernumerary Marker Chromosomes (sSMCs) exist. Materials & Methods: The identification of healthy sSMC carriers with euchromatic centromere-near (ECN) imbalances led to the following proposal: ECN-regions do not contain any dosage sensitive genes...
April 2018: Current Genomics
Takahiro Ohnishi, Marina Fujiwara, Akiko Tomaru, Tomoya Yoshinari, Yoshiko Sugita-Konishi
Cryopreservation methods for Kudoa septempunctata have not been established. This prevents an effective study of K. septempunctata, which cannot be artificially cultivated in the laboratory. In this study, we attempted to establish a cryopreservation method for K. septempunctata sporoplasm using Cellbanker® 1, a commercial preservation medium for mammalian cells. Spores were purified from the meat of Paralichthys olivaceus (olive flounder). These purified spores were suspended in Cellbanker® 1 and were stored at -80 °C for up to 16 months...
January 2017: Parasitology Research
Kodai Kusakisako, Tatsunori Masatani, Yurika Yada, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, Hiroki Maeda, Masami Mochizuki, Tetsuya Tanaka
In vitro cultivation and cryopreservation under liquid nitrogen have already been reported and established for Babesia bovis and B. bigemina parasites. Although the in vitro cultivation methods for B. gibsoni have been reported and established, the cryopreservation methods for this parasite have not been established completely. In this paper, we compared several freezing media for the cryopreservation of B. gibsoni parasite. The CELLBANKER(®) series (1 plus and 2), STEM-CELLBANKER(®), and CultureSure(®) were used for commercial freezing media; 10% dimethyl sulfoxide in 90% fetal bovine serum, 20% polyvinylpyrrolidone in phosphate-buffered saline (established for bovine Babesia parasites), and 28% glycerol supplemented with 3% sorbitol and 0...
February 24, 2016: Parasitology International
Takahisa Shimazu, Yuka Mori, Atsuko Takahashi, Hajime Tsunoda, Arinobu Tojo, Tokiko Nagamura-Inoue
BACKGROUND AIMS: Human umbilical cord (UC) has become a notable source for mesenchymal stromal cells (MSCs) that can migrate to areas of inflammation and damaged tissue and can suppress excess immune reactions and to repair, respectively. Although UC is a solid tissue, there are several advantages, including repeatable uses from the same donor sample when needed and the possibility of future explorations for cells with unknown potential, if we could cryopreserve the UC as a living tissue material...
May 2015: Cytotherapy
Shaymaa Subhi Hussein, Katharina Kreskowski, Monika Ziegler, Elisabeth Klein, Ahmed B Hamid, Nadezda Kosyakova, Marianne Volleth, Thomas Liehr, Xiaobo Fan, Katja Piaszinski
Mosaicism is present in more than 50% of the cases with small supernumerary marker chromosomes (sSMCs) and karyotype 47,XX,+mar/46,XX or 47,XY,+mar/46,XY. Recently we provided first evidence that the mitotic stability of sSMC is dependent on their structure, i.e. their shape. Thus, here we performed a long term in vitro study on 12 selected cell lines from the Else Kröner-Fresenius-sSMC-cellbank ( to test mitotic sSMC stability systematically. The obtained results showed that inverted duplicated shaped and also the so-called complex sSMCs (group 1) are by far more stable, than centric-minute- or ring-shaped sSMCs (groups 2)...
December 1, 2014: Gene
Shahla Hamza Al-Saqi, Mohammed Saliem, Hernan Concha Quezada, Åsa Ekblad, Aino Fianu Jonasson, Outi Hovatta, Cecilia Götherström
Mesenchymal stem cells (MSCs) have vast potential in cell therapy, and are experimentally used in the clinic. Therefore, it is critical to find a serum- and xeno-free cryopreservation method. The aim of this study was to compare two serum- and xeno-free cryoprotectants for MSCs. Adipose tissue MSCs (Ad-MSCs) and bone marrow MSCs (BM-MSCs) were cryopreserved in two cryoprotectants: the defined serum- and xeno-free STEM-CELLBANKER™ (CB) and 10 % dimethyl sulfoxide (DMSO) in a xeno-free serum replacement cell culture medium and compared to non-cryopreserved MSCs...
June 2015: Cell and Tissue Banking
Hannes Spittel, Florian Kubek, Katharina Kreskowski, Monika Ziegler, Elisabeth Klein, Ahmed B Hamid, Nadezda Kosyakova, Gopakumar Radhakrishnan, Annelore Junge, Peter Kozlowski, Berndt Schulze, Thomas Martin, Dagmar Huhle, Karl Mehnert, Laura Rodríguez, Mehmet A Ergun, Catherine Sarri, Mariela Militaru, Fedora Stipoljev, Hanne Tittelbach, Faezeh Vasheghani, Marcelo de Bello Cioffi, Shaymaa S Hussein, Xiaobo Fan, Marianne Volleth, Thomas Liehr
Small supernumerary marker chromosomes (sSMC) are known for being present in mosaic form as 47,+mar/46 in >50% of the cases with this kind of extra chromosomes. However, no detailed studies have been done for the mitotic stability of sSMC so far, mainly due to the lack of a corresponding in vitro model system. Recently, we established an sSMC-cell bank (Else Kröner-Fresenius-sSMC-cellbank) with >150 cell lines. Therefore, 93 selected sSMC cases were studied here for the presence of the corresponding marker chromosomes before and after Epstein-Barr virus-induced immortalization...
2014: Cytogenetic and Genome Research
Yoshitaka Miyamoto, Koichi Oishi, Hiroshi Yukawa, Hirofumi Noguchi, Masahiro Sasaki, Hisashi Iwata, Shuji Hayashi
Adipose tissue-derived stem/progenitor cells (ASCs) have attracted attention as a cell source that replaces marrow stromal cells (MSCs); ASCs may thus have applications in both regenerative medicine and cell transplantation. These medical treatments, however, require a high-quality supply of human ASCs. Therefore, the cryopreservation methods have been improved by changing a component of a cryopreservation medium. Sericin, a protein hydrolysate (with an average molecular weight of 30 kDa) is very rich in serine...
2012: Cell Transplantation
Mohammed Saliem, Frida Holm, Rosita Bergström Tengzelius, Carl Jorns, Lisa-Mari Nilsson, Bo-Göran Ericzon, Ewa Ellis, Outi Hovatta
AIM: To optimize a xeno-free cryopreservation protocol for primary human hepatocytes. METHODS: The demand for cryopreserved hepatocytes is increasing for both clinical and research purposes. Despite several hepatocyte cryopreservation protocols being available, improvements are urgently needed. We first compared controlled rate freezing to polystyrene box freezing and did not find any significant change between the groups. Using the polystyrene box freezing, we compared two xeno-free freezing solutions for freezing of primary human hepatocytes: a new medium (STEM-CELLBANKER, CB), which contains dimethylsulphoxide (DMSO) and anhydrous dextrose, both permeating and non-permeating cryoprotectants, and the frequently used DMSO - University of Wisconsin (DMSO-UW) medium...
May 27, 2012: World Journal of Hepatology
Wilhelm G Dirks, Hans G Drexler
The main prerequisition for any research, development, or production programs involving cell lines is whether a cell line is authentic or not. Microsatellites in the human genome harboring short tandem repeat (STR) DNA markers allow the identification of individual cell lines at the DNA level. Polymerase chain reaction (PCR) amplification of eight highly polymorphic microsatellite STR loci and gender determination have been proven to be the best tools for screening the uniqueness of DNA profiles in an STR database...
2011: Methods in Molecular Biology
Qilin Xi, Shaogang Wang, Zhangqun Ye, Jihong Liu, Xiao Yu, Zhaowei Zhu, Shiqiang Su, Jian Bai, Cong Li
UNLABELLED: What’s known on the subject? and What does the study add? Experimental data have shown that VDR overexpression in the duodenum and kidney cortex is a biological characteristic of genetic hypercalciuric stone-forming rats (GHS rat), and a link between idiopathic calcium stone formation and the microstatellite marker D12S339 (near the VDR locus) has been proven in humans. Our study shows that VDR can positively regulate the mRNA and protein expression of TRPV5, calbindin-D28k and PMCA1b in NRK cell lines...
April 2011: BJU International
Jun Arikura, Naoya Kobayashi, Teru Okitsu, Hirofumi Noguchi, Toshinori Totsugawa, Takamasa Watanabe, Toshihisa Matsumura, Masanobu Maruyama, Yoshikazu Kosaka, Noriaki Tanaka, Kazuhiko Onodera, Shinichi Kasai
Cryopreserved hepatocytes are a ready source of metabolic and synthetic functions for hepatocyte transplantation and bioartificial livers. In this study, we evaluated a cytoprotective effect of University of Wisconsin (UW) solution during cryopreservation of rat hepatocytes. We also investigated the feasibility of lentivirus-based gene transfer into thawed hepatocytes after cryopreservation. Primary rat hepatocytes were isolated using a two-step collagenase perfusion technique, and the resulting hepatocytes with more than 85% viability assessed by a trypan blue exclusion test were subjected to the present study...
2002: Journal of Hepato-biliary-pancreatic Surgery
K Hoshi, Y Kanno, H Katayose, K Yanagida, R Suzuki, A Sato
PURPOSE: The effect of coculturing mouse embryos with cryopreserved human oviduct epithelial cells was investigated. The cryopreserved cells in Cellbanker were thawed and cultured in Richard D. Goldsby culture medium to establish monolayers. Two-cell-stage mouse embryos were cultured alone (control group) or cocultured with monolayers established from cryopreserved cells (cryopreserved coculture group) or from fresh cells (fresh coculture group). The rates of embryo development and the qualities of the blastocysts in the three groups were compared...
August 1994: Journal of Assisted Reproduction and Genetics
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