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hans-dieter volk

Mohamed Abou-El-Enein, Hans-Dieter Volk, Petra Reinke
Cellular therapies have potential to treat a wide range of diseases with autologous immunotherapies showing unprecedented therapeutic promise in clinical trials. Such therapies are mainly developed by academic researchers applying small-scale production, targeting rare and unmet medical needs. Here we highlight the clinical translation of immunotherapy product in an academic setting which may serve as a success model for early academic development of cell-based therapeutics. This article is protected by copyright...
October 6, 2016: Clinical Pharmacology and Therapeutics
Alberto Sada Japp, Kerstin Hoffmann, Stephan Schlickeiser, Rainer Glauben, Christos Nikolaou, Holden T Maecker, Julian Braun, Nadine Matzmohr, Birgit Sawitzki, Britta Siegmund, Andreas Radbruch, Hans-Dieter Volk, Marco Frentsch, Desiree Kunkel, Andreas Thiel
A great part of our knowledge on mammalian immunology has been established in laboratory settings. The use of inbred mouse strains enabled controlled studies of immune cell and molecule functions in defined settings. These studies were usually performed in specific-pathogen free (SPF) environments providing standardized conditions. In contrast, mammalians including humans living in their natural habitat are continuously facing pathogen encounters throughout their life. The influences of environmental conditions on the signatures of the immune system and on experimental outcomes are yet not well defined...
July 12, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Mohamed Abou-El-Enein, Gerhard Bauer, Nicholas Medcalf, Hans-Dieter Volk, Petra Reinke
Cell therapies, especially autologous therapies, pose significant challenges to researchers who wish to move from small, probably academic, methods of manufacture to full commercial scale. There is a dearth of reliable information about the costs of operation, and this makes it difficult to predict with confidence the investment needed to translate the innovations to the clinic, other than as small-scale, clinician-led prescriptions. Here, we provide an example of the results of a cost model that takes into account the fixed and variable costs of manufacture of one such therapy...
August 2016: Cytotherapy
Leif G Hanitsch, Madlen Löbel, Jan Florian Mieves, Sandra Bauer, Nina Babel, Brunhilde Schweiger, Kirsten Wittke, Patricia Grabowski, Hans-Dieter Volk, Carmen Scheibenbogen
BACKGROUND: Immunization against seasonal influenza with inactivated vaccine is recommended for patients with common variable immunodeficiency (CVID). However, humoral vaccine response in CVID patients is frequently impaired and current knowledge on T cell vaccine response in CVID and other patients with antibody deficiency is poor. OBJECTIVE: In the present study, we comparatively analyzed the antibody and T cellular immune response of patients with CVID and unclassified antibody deficiency to influenza vaccination in the season 2013-2014...
May 5, 2016: Vaccine
Marije Baas, Alix Besançon, Tania Goncalves, Fabrice Valette, Hideo Yagita, Birgit Sawitzki, Hans-Dieter Volk, Emmanuelle Waeckel-Enée, Benedita Rocha, Lucienne Chatenoud, Sylvaine You
CD8(+) T cell anergy is a critical mechanism of peripheral tolerance, poorly investigated in response to immunotherapy. Here, using a pancreatic islet allograft model and CD3 antibody therapy, we showed, by single cell gene profiling, that intragraft CD8(+) lymphocytes coexpressing granzyme B and perforin were selectively depleted through the Fas/FasL pathway. This step led to long-standing anergy of the remaining CD8(+) T cells marked by the absence of cytotoxic/inflammatory gene expression also confirmed by transcriptome analysis...
2016: ELife
Ellen Witte, Georgios Kokolakis, Katrin Witte, Katarzyna Warszawska, Markus Friedrich, Demetrios Christou, Stefan Kirsch, Wolfram Sterry, Hans-Dieter Volk, Robert Sabat, Kerstin Wolk
UNLABELLED: Psoriasis is considered as a model for chronic immune-mediated disorders. Th17-cells are pivotal players in those diseases. Recently, we demonstrated that Th17-cells produce interleukin (IL)-29 and that IL-29 is highly present in psoriatic lesions. Whether IL-29, with its action on epithelial cells and melanocytes, contributes to psoriasis pathogenesis, was unknown so far. Analysis of IL-29-treated human keratinocytes revealed induction of the chemokines CXCL10, CXCL11, and, to a much lesser extent, CXCL9...
April 2016: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
Odilo Engel, Levent Akyüz, Andrey C da Costa Goncalves, Katarzyna Winek, Claudia Dames, Mareike Thielke, Susanne Herold, Chotima Böttcher, Josef Priller, Hans Dieter Volk, Ulrich Dirnagl, Christian Meisel, Andreas Meisel
BACKGROUND AND PURPOSE: Temporary immunosuppression has been identified as a major risk factor for the development of pneumonia after acute central nervous system injury. Although overactivation of the sympathetic nervous system was previously shown to mediate suppression of systemic cellular immune responses after stroke, the role of the parasympathetic cholinergic anti-inflammatory pathway in the antibacterial defense in lung remains largely elusive. METHODS: The middle cerebral artery occlusion model in mice was used to examine the influence of the parasympathetic nervous system on poststroke immunosuppression...
November 2015: Stroke; a Journal of Cerebral Circulation
Sabrina Guenther, Madlen Loebel, Agnes A Mooslechner, Michael Knops, Leif G Hanitsch, Patricia Grabowski, Kirsten Wittke, Christian Meisel, Nadine Unterwalder, Hans-Dieter Volk, Carmen Scheibenbogen
Chronic fatigue syndrome (CFS) is a severe disease characterized by various symptoms of immune dysfunction. CFS onset is typically with an infection and many patients suffer from frequently recurrent viral or bacterial infections. Immunoglobulin and mannose binding lectin (MBL) deficiency are frequent causes for increased susceptibility to infections. In this study we retrospectively analysed 300 patients with CFS for immunoglobulin and MBL levels, and B-cell subset frequencies. 25% of the CFS patients had decreased serum levels of at least one antibody class or subclass with IgG3 and IgG4 subclass deficiencies as most common phenotypes...
October 2015: Human Immunology
Katrin Witte, Egon Koch, Hans-Dieter Volk, Kerstin Wolk, Robert Sabat
Pelargonium sidoides is a medical herb and respective extracts are used very frequently for the treatment of respiratory tract infections. However, the effects of Pelargonium sidoides and a special extract prepared from its roots (EPs 7630) on human immune cells are not fully understood. Here we demonstrate that EPs 7630 induced a rapid and dose-dependent production of TNF-α, IL-6, and IL-10 by human blood immune cells. This EPs 7630-induced cytokine profile was more pro-inflammatory in comparison with the profile induced by viral or bacterial infection-mimicking agents...
2015: PloS One
Madlen Loebel, Patricia Grabowski, Harald Heidecke, Sandra Bauer, Leif G Hanitsch, Kirsten Wittke, Christian Meisel, Petra Reinke, Hans-Dieter Volk, Øystein Fluge, Olav Mella, Carmen Scheibenbogen
Infection-triggered disease onset, chronic immune activation and autonomic dysregulation in CFS point to an autoimmune disease directed against neurotransmitter receptors. Autoantibodies against G-protein coupled receptors were shown to play a pathogenic role in several autoimmune diseases. Here, serum samples from a patient cohort from Berlin (n=268) and from Bergen with pre- and post-treatment samples from 25 patients treated within the KTS-2 rituximab trial were analysed for IgG against human α and β adrenergic, muscarinic (M) 1-5 acetylcholine, dopamine, serotonin, angiotensin, and endothelin receptors by ELISA and compared to a healthy control cohort (n=108)...
February 2016: Brain, Behavior, and Immunity
Hong Lei, Katharina Schmidt-Bleek, Anke Dienelt, Petra Reinke, Hans-Dieter Volk
Regulatory T cells (Tregs) offer new immunotherapeutic options to control undesired immune reactions, such as those in transplant rejection and autoimmunity. In addition, tissue repair and regeneration depend on a multitude of tightly regulated immune and non-immune cells and signaling molecules. There is mounting evidence that adequate innate responses, and even more importantly balanced adaptive immune responses, are key players in the tissue repair and regeneration processes, even in absence of any immune-related disease or infection...
2015: Frontiers in Pharmacology
Angela Mensen, Torben Krause, Leif Gunnar Hanitsch, Christian Meisel, Maximilian E Kleint, Hans-Dieter Volk, Il-Kang Na, Carmen Scheibenbogen
Primary selective IgM deficiency (sIgM) is characterized by diminished serum IgM, infections and autoimmunity. Although there is some evidence of B-cell defects the pathogenesis of sIgM is poorly understood. We determined peripheral B-cell subsets and IgM-expression levels in 31 adult sIgM patients by flow cytometry. In a subset of patients B-cell subset alterations and antibody-secreting cells were determined by flow cytometry and ELISpot assay after in vitro differentiation.Patients had significantly increased transitional, decreased IgM only, switched and non-switched memory B cells and decreased membrane IgM-expression levels on memory B-cell subsets compared to healthy controls...
December 2015: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Madlen Löbel, Agnes Anna Mooslechner, Sandra Bauer, Sabrina Günther, Anne Letsch, Leif G Hanitsch, Patricia Grabowski, Christian Meisel, Hans-Dieter Volk, Carmen Scheibenbogen
BACKGROUND: Chronic fatigue syndrome (CFS) is considered as a neuroimmunological disease but the etiology and pathophysiology is poorly understood. Patients suffer from sustained exhaustion, cognitive impairment and an increased sensitivity to pain and sensory stimuli. A subset of patients has frequent respiratory tract infections (RRTI). Dysregulation of the sympathetic nervous system and an association with genetic variations in the catechol-O-methyltransferase (COMT) and glucocorticoid receptor genes influencing sympathetic and glucocorticoid metabolism were reported in CFS...
2015: Journal of Translational Medicine
Christine Consentius, Petra Reinke, Hans-Dieter Volk
Mesenchymal stromal cells (MSC) are promising candidates for supporting regeneration and suppressing undesired immune reactivity. Although autologous MSC have been most commonly used for clinical trials, data on application of allogeneic MHC-unmatched MSC were reported. The usage of MSC as an 'off-the-shelf' product would have several advantages; however, it is an immunological challenge. The preclinical studies on the (non)immunogenicity of MSC are contradictory and, unfortunately, solid data from clinical trials are missing...
2015: Regenerative Medicine
Michael Schmueck-Henneresse, Radwa Sharaf, Katrin Vogt, Benjamin J D Weist, Sybille Landwehr-Kenzel, Henrike Fuehrer, Anke Jurisch, Nina Babel, Cliona M Rooney, Petra Reinke, Hans-Dieter Volk
Memory T cells expressing stem cell-like properties have been described recently. The capacity of self-renewal and differentiation into various memory/effector subsets make them attractive for adoptive T cell therapy to combat severe virus infections and tumors. The very few reports on human memory stem T cells (T(SCM)) are restricted to analyses on polyclonal T cells, but extensive data on Ag-specific T(SCM )are missing. This might be due to their very low frequency limiting their enrichment and characterization...
June 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
Leif G Hanitsch, Madlen Löbel, Holger Müller-Redetzky, Mariana Schürmann, Norbert Suttorp, Nadine Unterwalder, Ulrike Mönnich, Christian Meisel, Kirsten Wittke, Hans-Dieter Volk, Carmen Scheibenbogen, Uwe Kölsch
PURPOSE: Since we described for the first time a patient with IgG4 autoantibodies to IFN-γ more than 10 years ago, many patients with IFN-γ IgG4 autoantibodies have been described, mostly in Mongolian/ Asian patients with a particular HLA background and in association with disseminated nontuberculous mycobacterial infections. Very recently, the first Caucasian US patient was reported and we now present the case of a 65-year old Caucasian woman with severe disseminated Mycobacterium avium infection, cerebral toxoplasmosis and salmonella sepsis who was tested positive for IFN-γ deficiency due to unusual anti-IFN-γ IgG1 autoantibodies...
May 2015: Journal of Clinical Immunology
Amanda B Spurdle, Fergus J Couch, Michael T Parsons, Lesley McGuffog, Daniel Barrowdale, Manjeet K Bolla, Qin Wang, Sue Healey, Rita Schmutzler, Barbara Wappenschmidt, Kerstin Rhiem, Eric Hahnen, Christoph Engel, Alfons Meindl, Nina Ditsch, Norbert Arnold, Hansjoerg Plendl, Dieter Niederacher, Christian Sutter, Shan Wang-Gohrke, Doris Steinemann, Sabine Preisler-Adams, Karin Kast, Raymonda Varon-Mateeva, Steve Ellis, Debra Frost, Radka Platte, Jo Perkins, D Gareth Evans, Louise Izatt, Ros Eeles, Julian Adlard, Rosemarie Davidson, Trevor Cole, Giulietta Scuvera, Siranoush Manoukian, Bernardo Bonanni, Frederique Mariette, Stefano Fortuzzi, Alessandra Viel, Barbara Pasini, Laura Papi, Liliana Varesco, Rosemary Balleine, Katherine L Nathanson, Susan M Domchek, Kenneth Offitt, Anna Jakubowska, Noralane Lindor, Mads Thomassen, Uffe Birk Jensen, Johanna Rantala, Åke Borg, Irene L Andrulis, Alexander Miron, Thomas V O Hansen, Trinidad Caldes, Susan L Neuhausen, Amanda E Toland, Heli Nevanlinna, Marco Montagna, Judy Garber, Andrew K Godwin, Ana Osorio, Rachel E Factor, Mary B Terry, Timothy R Rebbeck, Beth Y Karlan, Melissa Southey, Muhammad Usman Rashid, Nadine Tung, Paul D P Pharoah, Fiona M Blows, Alison M Dunning, Elena Provenzano, Per Hall, Kamila Czene, Marjanka K Schmidt, Annegien Broeks, Sten Cornelissen, Senno Verhoef, Peter A Fasching, Matthias W Beckmann, Arif B Ekici, Dennis J Slamon, Stig E Bojesen, Børge G Nordestgaard, Sune F Nielsen, Henrik Flyger, Jenny Chang-Claude, Dieter Flesch-Janys, Anja Rudolph, Petra Seibold, Kristiina Aittomäki, Taru A Muranen, Päivi Heikkilä, Carl Blomqvist, Jonine Figueroa, Stephen J Chanock, Louise Brinton, Jolanta Lissowska, Janet E Olson, Vernon S Pankratz, Esther M John, Alice S Whittemore, Dee W West, Ute Hamann, Diana Torres, Hans Ulrich Ulmer, Thomas Rüdiger, Peter Devilee, Robert A E M Tollenaar, Caroline Seynaeve, Christi J Van Asperen, Diana M Eccles, William J Tapper, Lorraine Durcan, Louise Jones, Julian Peto, Isabel dos-Santos-Silva, Olivia Fletcher, Nichola Johnson, Miriam Dwek, Ruth Swann, Anita L Bane, Gord Glendon, Anna M Mulligan, Graham G Giles, Roger L Milne, Laura Baglietto, Catriona McLean, Jane Carpenter, Christine Clarke, Rodney Scott, Hiltrud Brauch, Thomas Brüning, Yon-Dschun Ko, Angela Cox, Simon S Cross, Malcolm W R Reed, Jan Lubinski, Katarzyna Jaworska-Bieniek, Katarzyna Durda, Jacek Gronwald, Thilo Dörk, Natalia Bogdanova, Tjoung-Won Park-Simon, Peter Hillemanns, Christopher A Haiman, Brian E Henderson, Fredrick Schumacher, Loic Le Marchand, Barbara Burwinkel, Frederik Marme, Harald Surovy, Rongxi Yang, Hoda Anton-Culver, Argyrios Ziogas, Maartje J Hooning, J Margriet Collée, John W M Martens, Madeleine M A Tilanus-Linthorst, Hermann Brenner, Aida Karina Dieffenbach, Volke Arndt, Christa Stegmaier, Robert Winqvist, Katri Pylkäs, Arja Jukkola-Vuorinen, Mervi Grip, Annika Lindblom, Sara Margolin, Vijai Joseph, Mark Robson, Rohini Rau-Murthy, Anna González-Neira, José Ignacio Arias, Pilar Zamora, Javier Benítez, Arto Mannermaa, Vesa Kataja, Veli-Matti Kosma, Jaana M Hartikainen, Paolo Peterlongo, Daniela Zaffaroni, Monica Barile, Fabio Capra, Paolo Radice, Soo H Teo, Douglas F Easton, Antonis C Antoniou, Georgia Chenevix-Trench, David E Goldgar
INTRODUCTION: The distribution of histopathological features of invasive breast tumors in BRCA1 or BRCA2 germline mutation carriers differs from that of individuals with no known mutation. Histopathological features thus have utility for mutation prediction, including statistical modeling to assess pathogenicity of BRCA1 or BRCA2 variants of uncertain clinical significance. We analyzed large pathology datasets accrued by the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA) and the Breast Cancer Association Consortium (BCAC) to reassess histopathological predictors of BRCA1 and BRCA2 mutation status, and provide robust likelihood ratio (LR) estimates for statistical modeling...
2014: Breast Cancer Research: BCR
Hans-Dieter Volk, Molly M Stevens, David J Mooney, David W Grainger, Georg N Duda
Translation in an academic environment requires a support system--people, goals, models, partnerships, and infrastructures--that will push promising basic science and technology projects forward into the clinic.
April 8, 2015: Science Translational Medicine
Caroline Schmidt-Lucke, Thomas Zobel, Sonja Schrepfer, Uwe Kuhl, Dong Wang, Karin Klingel, Peter Moritz Becher, Henry Fechner, Tanja Pozzuto, Sophie Van Linthout, Dirk Lassner, Frank Spillmann, Felicitas Escher, Sebastian Holinski, Hans-Dieter Volk, Heinz-Peter Schultheiss, Carsten Tschope
Human parvovirus B19 (B19V) is a common pathogen in microvascular disease and cardiomyopathy, owing to infection of endothelial cells. B19V replication, however, is almost restricted to erythroid progenitor cells (ErPCs). Endothelial regeneration attributable to bone marrow-derived circulating angiogenic cells (CACs) is a prerequisite for organ function. Because of many similarities of ErPCs and CACs, we hypothesized that B19V is a perpetrator of impaired endogenous endothelial regeneration. B19V DNA and messenger RNA from endomyocardial biopsy specimens, bone marrow specimens, and circulating progenitor cells were quantified by polymerase chain reaction analysis...
October 1, 2015: Journal of Infectious Diseases
Faouzi Braza, Emilie Dugast, Ivo Panov, Chloé Paul, Katrin Vogt, Annaick Pallier, Mélanie Chesneau, Daniel Baron, Pierrick Guerif, Hong Lei, David-Axel Laplaud, Hans-Dieter Volk, Nicolas Degauque, Magali Giral, Jean-Paul Soulillou, Birgit Sawitzki, Sophie Brouard
The role of Foxp3(+) regulatory T cells (Tregs) in operational tolerance remains elusive, as initial results revealed an increased frequency of this subset in tolerant patients but no functional differences compared with immunosuppressed recipients. In addition, recent studies of regulatory B cells strongly suggest that Tregs may not have a central role in kidney transplantation tolerance. However, recent investigations of the crucial role of Foxp3 demethylation in Treg function and the possibility of identifying distinct Foxp3 T cell subsets prompted us to more thoroughly characterize Tregs in operationally tolerant patients...
August 2015: Journal of the American Society of Nephrology: JASN
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