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https://www.readbyqxmd.com/read/27913649/identification-and-function-of-fibrocytes-in-skeletal-muscle-injury-repair-and-muscular-dystrophy
#1
Xingyu Wang, Wanming Zhao, Richard M Ransohoff, Lan Zhou
We identified and characterized the function of CD45(+)/collagen I(+) fibrocytes in acutely injured skeletal muscle of wild-type (WT) and Ccr2(-/-) mice, and in quadriceps and diaphragm muscles of mdx(5cv) mice, a mouse model for Duchenne muscular dystrophy. Fibrocytes were not detected in peripheral blood of WT mice after acute muscle injury or mdx(5cv) mice. Fibrocytes were detected in acutely injured muscles and in mdx(5cv) quadriceps and diaphragm muscles. These cells expressed F4/80 and CCR2, and they were mostly Ly6C(lo) They expressed a low level of collagens but a high level of profibrotic growth factors as compared with i...
December 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27911416/the-indirect-neuron-astrocyte-coculture-assay-an-in-vitro-set-up-for-the-detailed-investigation-of-neuron-glia-interactions
#2
Christine Gottschling, Egor Dzyubenko, Maren Geissler, Andreas Faissner
Proper neuronal development and function is the prerequisite of the developing and the adult brain. However, the mechanisms underlying the highly controlled formation and maintenance of complex neuronal networks are not completely understood thus far. The open questions concerning neurons in health and disease are diverse and reaching from understanding the basic development to investigating human related pathologies, e.g., Alzheimer's disease and Schizophrenia. The most detailed analysis of neurons can be performed in vitro...
November 14, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27909436/evaluation-of-anti-inflammatory-effects-of-steroids-and-arthritis-related-biotherapies-in-an-in-vitro-coculture-model-with-immune-cells-and-synoviocytes
#3
Mélissa Noack, Ndiémé Ndongo-Thiam, Pierre Miossec
BACKGROUND: During rheumatoid arthritis (RA), steroids and biotherapies are used alone and combined. Efficacy has been established in clinical trials but their differential effects at the cellular level are less documented. The aim was to study these cellular effects using an in vitro model with synoviocytes interacting with peripheral blood mononuclear cells (PBMC) to reproduce the interactions in the RA synovium. METHODS: Activated-PBMC were cocultured with RA synoviocytes during 48 h...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27906587/differentiation-of-mouse-ovarian-stem-cells-toward-oocyte-like-structure-by-coculture-with-granulosa-cells
#4
Soraya Parvari, Hossein Yazdekhasti, Zahra Rajabi, Valliollah Gerayeli Malek, Tayebeh Rastegar, Mehdi Abbasi
An increasing body of evidence has confirmed existence and function of ovarian stem cells (OSCs). In this study, a novel approach on differentiation of OSCs into oocyte-like cells (OLCs) has been addressed. Recently, different methods have been recruited to isolate and describe aspects of OSCs, but newer and more convenient strategies in isolation are still growing. Herein, a morphology-based method was used to isolate OSCs. Cell suspension of mouse neonatal ovaries was cultured and formed colonies were harvested mechanically and cultivated on mouse embryonic fibroblasts...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27905502/immune-responsiveness-of-cd4-t-cells-during-streptococcus-suis-serotype-2-infection
#5
Marie-Pier Lecours, Corinne Letendre, Damian Clarke, Paul Lemire, Tristan Galbas, Marie-Odile Benoit-Biancamano, Jacques Thibodeau, Marcelo Gottschalk, Mariela Segura
The pathogenesis of Streptococcus suis infection, a major swine and human pathogen, is only partially understood and knowledge on the host adaptive immune response is critically scarce. Yet, S. suis virulence factors, particularly its capsular polysaccharide (CPS), enable this bacterium to modulate dendritic cell (DC) functions and potentially impair the immune response. This study aimed to evaluate modulation of T cell activation during S. suis infection and the role of DCs in this response. S. suis-stimulated total mouse splenocytes readily produced TNF-α, IL-6, IFN-γ, CCL3, CXCL9, and IL-10...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27901316/small-subcompartmentalized-microreactors-as-support-for-hepatocytes
#6
Yan Zhang, Marie Baekgaard-Laursen, Brigitte Städler
Mimicking specific structural or functional aspects of cells is considered a promising approach to substitute for missing or lost cellular functions. However, the interaction of such artificial assemblies with their biological counterparts including the exploitation of the activity of the synthetic partner remains thus-far a rather unexplored avenue. Herein, the assembly of active microreactors with similar size to hepatocytes is reported. These microreactors are successfully cocultured with hepatocytes into bionic tissue for up to 10 d...
November 30, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27898053/microbial-mutualism-dynamics-governed-by-dose-dependent-toxicity-of-cross-fed-nutrients
#7
Breah LaSarre, Alexandra L McCully, Jay T Lennon, James B McKinlay
Microbial interactions, including mutualistic nutrient exchange (cross-feeding), underpin the flow of energy and materials in all ecosystems. Metabolic exchanges are difficult to assess within natural systems. As such, the impact of exchange levels on ecosystem dynamics and function remains unclear. To assess how cross-feeding levels govern mutualism behavior, we developed a bacterial coculture amenable to both modeling and experimental manipulation. In this coculture, which resembles an anaerobic food web, fermentative Escherichia coli and photoheterotrophic Rhodopseudomonas palustris obligately cross-feed carbon (organic acids) and nitrogen (ammonium)...
November 29, 2016: ISME Journal
https://www.readbyqxmd.com/read/27891153/pahs-target-hematopoietic-linages-in-bone-marrow-through-cyp1b1-primarily-in-mesenchymal-stromal-cells-but-not-ahr-a-reconstituted-in-vitro-model
#8
Catherine M Rondelli, Michele Campaigne Larsen, Alhaji N'jai, Charles J Czuprynski, Colin R Jefcoate
7,12-Dimethylbenz(a)anthracene (DMBA) rapidly suppresses hematopoietic progenitors, measured as colony forming units (CFU), in mouse bone marrow (BM) leading to mature cell losses as replenishment fails. These losses are mediated by Cyp1b1, independent of the AhR, despite induction of Cyp1b1. BM mesenchymal progenitor cells (MPC) may mediate these responses since basal Cyp1b1 is minimally induced. PreB colony forming unit activity (PreB CFU) is lost within 24 hours in isolated BM cells (BMC) unless cocultured with cells derived from primary MPC (BMS2 line)...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27888017/circulating-cxcr5-cd4-t-cells-assist-in-the-survival-and-growth-of-primary-diffuse-large-b-cell-lymphoma-cells-through-interleukin-10-pathway
#9
Zhanshan Cha, Guangfang Qian, Yan Zang, Haihui Gu, Yanyan Huang, Lishuang Zhu, Jinqi Li, Yang Liu, Xiaohua Tu, Haihan Song, Baohua Qian
Diffuse large B cell lymphoma (DLBCL) is a common and aggressive cancer caused by the malignant transformation of B cells. Although it has been established that the follicular helper T (Tfh) cells play a central role in B cell development, little information is available on their involvement in DLBCL pathogenesis. We studied the role of the peripheral Tfh equivalent, the CXCR5(+) CD4(+) T cells, in DLBCL. Data showed that compared to CXCR5(-) CD4(+) T cells, CXCR5(+) CD4(+) T cells were significantly more effective at promoting the proliferation as well as inhibiting the apoptosis of primary autologous DLBCL tumor cells...
November 23, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27882459/analysis-of-proliferation-of-melanoma-cells-and-mesenchymal-stem-cells-in-co-culture-and-contribution-of-experimental-conditions-into-interpretation-of-the-results
#10
O F Kandarakov, E E Kopantseva, A V Belyavsky
A series of experiments on co-culturing of Mel IL melanoma cells and mesenhymal stem cells showed that these cells do not influence proliferation of each other, but we observed weaker adhesion of stromal stem cells to plastic in cocultures where with melanoma cells were grown on mesenhymal stem cells feeder. Cell proliferation was also considerably influenced by experimental conditions, which should be taken into account for correct interpretation of obtained results. The principles of experiments on co-culturing of cancer and stromal cells are formulated that take into account the most important factors influencing cell behavior and minimize the probability of artifact results...
November 23, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27872867/bone-marrow-mesenchymal-stem-cells-enhance-the-differentiation-of-human-switched-memory-b-lymphocytes-into-plasma-cells-in-serum-free-medium
#11
Guillaume Bonnaure, Catherine Gervais-St-Amour, Sonia Néron
The differentiation of human B lymphocytes into plasma cells is one of the most stirring questions with regard to adaptive immunity. However, the terminal differentiation and survival of plasma cells are still topics with much to be discovered, especially when targeting switched memory B lymphocytes. Plasma cells can migrate to the bone marrow in response to a CXCL12 gradient and survive for several years while secreting antibodies. In this study, we aimed to get closer to niches favoring plasma cell survival...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/27872864/f4-80-host-macrophages-are-a-barrier-to-murine-embryonic-stem-cell-derived-hematopoietic-progenitor-engraftment-in-vivo
#12
Heather L Thompson, Nico van Rooijen, Bryce T McLelland, Jennifer O Manilay
Understanding how embryonic stem cells and their derivatives interact with the adult host immune system is critical to developing their therapeutic potential. Murine embryonic stem cell-derived hematopoietic progenitors (ESHPs) were generated via coculture with the bone marrow stromal cell line, OP9, and then transplanted into NOD.SCID.Common Gamma Chain (NSG) knockout mice, which lack B, T, and natural killer cells. Compared to control mice transplanted with adult lineage-negative bone marrow (Lin(-) BM) progenitors, ESHP-transplanted mice attained a low but significant level of donor hematopoietic chimerism...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/27870715/posttranscriptional-control-of-pd-l1-expression-by-17%C3%AE-estradiol-via-pi3k-akt-signaling-pathway-in-er%C3%AE-positive-cancer-cell-lines
#13
Lingyun Yang, Feng Huang, Jiandong Mei, Xun Wang, Qiuyang Zhang, Hongjing Wang, Mingrong Xi, Zongbing You
OBJECTIVE: Estrogen is a well-known oncogenic driver in endometrial (ECs) and breast cancers (BCs). Programmed cell death protein 1 (PD-1) and its ligands PD-1 Ligand 1 (PD-L1) and PD-L2 have been shown to mediate immune evasion of the tumor cells. The purpose of the present study was to assess the effects of estrogen on PD-L1 and PD-L2 expression in EC and BC cell lines. METHODS: 17β-Estradiol (E2)-induced expression of PD-L1 and PD-L2 and possible signaling pathway were investigated in EC and BC cells...
November 17, 2016: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/27869186/regulation-of-anticancer-styrylpyrone-biosynthesis-in-the-medicinal-mushroom-inonotus-obliquus-requires-thioredoxin-mediated-transnitrosylation-of-s-nitrosoglutathione-reductase
#14
Yanxia Zhao, Meihong He, Jianing Ding, Qi Xi, Gary J Loake, Weifa Zheng
The medicinal macrofungus Inonotus obliquus widely utilized as folk medicine in Russia and Baltic countries is a source of phenylpropanoid-derived styrylpyrone polyphenols that can inhibit tumor proliferation. Insights into the regulatory machinery that controls I. obliquus styrylpyrone polyphenol biosynthesis will enable strategies to increase the production of these molecules. Here we show that Thioredoxin (Trx) mediated transnitrosylation of S-nitrosoglutathione reductase (GSNOR) underpins the regulation of styrylpyrone production, driven by nitric oxide (NO) synthesis triggered by P...
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27864016/generation-of-antigen-specific-cytotoxic-t-lymphocytes-with-activated-b-cells
#15
Sun Ok Yun, Hee Young Shin, Chang-Yuil Kang, Hyoung Jin Kang
BACKGROUND AIMS: Dendritic cells are well known as the most potent antigen-presenting cells. Nonetheless, their use in immunotherapy has been limited by the time-consuming and laborious steps involved in their generation in vitro. Therefore, much attention has been placed on alternative antigen-presenting cells that are relatively more convenient to manipulate. METHODS: In this study, the efficacy of B cells as antigen-presenting cells, compared with dendritic cells, in the induction of cytotoxic T lymphocytes against cytomegalovirus-specific antigens was evaluated...
November 15, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27862395/the-nuclear-and-developmental-competence-of-cumulus-oocyte-complexes-is-enhanced-by-three-dimensional-coculture-with-conspecific-denuded-oocytes-during-in-vitro-maturation-in-the-domestic-cat-model
#16
M G Morselli, G C Luvoni, P Comizzoli
The objective of the study was to assess the efficacy of coculture with conspecific cumulus-denuded oocytes (CDOs) during in vitro maturation in a three-dimensional system of barium alginate microcapsules on the in vitro embryo development of domestic cat cumulus-oocyte complexes (COCs). In Experiment I, COCs were cocultured with conspecific CDOs or cultured separately in a 3D system for 24 hr of in vitro maturation, before assessing the meiotic progression. In Experiment II, the in vitro fertilization of COCs and CDOs was carried out with chilled epididymal spermatozoa and the presumptive zygotes were cultured in vitro separately for 7 days in 3D microcapsules before assesment of embryonic development...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27860372/biomaterials-and-culture-technologies-for-regenerative-therapy-of-liver-tissue
#17
Roman A Perez, Cho-Rok Jung, Hae-Won Kim
Regenerative approach has emerged to substitute the current extracorporeal technologies for the treatment of diseased and damaged liver tissue. This is based on the use of biomaterials that modulate the responses of hepatic cells through the unique matrix properties tuned to recapitulate regenerative functions. Cells in liver preserve their phenotype or differentiate through the interactions with extracellular matrix molecules. Therefore, the intrinsic properties of the engineered biomaterials, such as stiffness and surface topography, need to be tailored to induce appropriate cellular functions...
November 18, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27859361/an-in-vitro-scaffold-free-epithelial-fibroblast-coculture-model-for-the-larynx
#18
Tanaya Walimbe, Alyssa Panitch, M Preeti Sivasankar
OBJECTIVES/HYPOTHESIS: Physiologically relevant, well-characterized in vitro vocal fold coculture models are needed to test the effects of various challenges and therapeutics on vocal fold physiology. We characterize a healthy state coculture model, created by using bronchial/tracheal epithelial cells and immortalized vocal fold fibroblasts. We also demonstrate that this model can be induced into a fibroplastic state to overexpress stress fibers using TGFβ1. STUDY DESIGN: In vitro...
November 16, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27858354/coculture-assays-for-endothelial-cells-mural-cells-interactions
#19
Diana M Sánchez-Palencia, Alex Bigger-Allen, Magali Saint-Geniez, Joseph F Arboleda-Velásquez, Patricia A D'Amore
Coculture assays allow the investigation of the role of endothelial cell and mural cell interactions in small vessel development and function. Different setups for coculture can be used to assay questions of interest. We include here methods for direct coculture, indirect coculture, and coculture in a three-dimensional extracellular matrix scaffold for studies of either a simple and direct association between the two cell types, the exchange of soluble molecules, or the interaction within a biomimetic tissue microenvironment...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27852364/-the-expression-of-macrophage-migration-inhibitory-factor-in-obstructive-sleep-apnea-hypopnea-syndrome-and-its-injury-to-endothelial-cells
#20
P Yuan, W J Wang, D F Li, Q Ou, X L Gao
Objective: To study the expression of macrophage migration inhibitory factor (MIF) in obstructive sleep apnea hypopnea syndrome (OSAHS) and its injury to endothelial cells. Methods: According to the PSG test results, subjects who were the first time to take PSG examination without treatment (n=71) were divided into a control group (n=20), a mild OSAHS group (n=19), a moderate OSAHS group (n=15) and severe OSAHS group (n=17). For each patients, 4 ml fasting peripheral blood was obtained when PSG was finished around 6: 30 in the next morning, and the MIF level in plasma was detected with the ELISA method...
November 12, 2016: Chinese Journal of Tuberculosis and Respiratory Diseases
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