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I V Kravchenko, V A Furalev, V O Popov
We studied the effect of myofibrils on proliferation and differentiation of myoblasts cocultured with macrophages as well as the effect of incubation of macrophages with myofibrils on the expression by macrophages of the compounds that are cytokines for muscle cells. In the cocultures, macrophages stimulated the proliferation of myoblasts. Myofibrils greatly enhanced the stimulating effect of macrophages, whereas lipopolysaccharide (LPS) completely abolished it. The culture medium conditioned by macrophages activated the proliferation of myoblasts that were incubated with myofibrils but inhibited it when myoblasts were incubated with LPS...
March 2018: Doklady. Biochemistry and Biophysics
L B Buravkova, E R Andreeva, M V Lobanova, E V Cotnezova, A I Grigoriev
The dynamics of the expression of genes encoding adhesion molecules, molecules of the connective tissue matrix, and its remodeling enzymes was studied in multipotent mesenchymal stromal cells (MSCs) from human adipose tissue after interaction with cord blood hematopoietic progenitors (HSPCs). An upregulation of ICAM1 and VCAM1, directly proportional to the coculture time (24-72 h), was found. After 72 h of culturing, a downregulation of the genes encoding the majority of matrix molecules (SPP1; COL6A2,7A1; MMP1,3; TIMP1,3; and HAS1) and cell-matrix adhesion molecules (ITGs) was revealed...
March 2018: Doklady. Biochemistry and Biophysics
Fatemeh Soleimanifar, Yousef Mortazavi, Samad Nadri, Maryam Islami, Saeid Vakilian
Coculture systems are widely used in tissue engineering to mimic cell-cell interactions between different populations. This study aimed to find an improved and convenient system for the corneal epithelial differentiation of conjunctiva derived mesenchymal stem cells (CJMSCs). Thus, the cells were used to reconstruct corneal epithelial cells. Obtained by flow cytometry data, 51.9% of isolated CJMSCs were immune reactive for SSEA4+ antibody which are more potent to differentiate into corneal epithelial cells...
May 15, 2018: Biologicals: Journal of the International Association of Biological Standardization
Kyoko Mashima, Shinichi Takahashi, Kazushi Minami, Yoshikane Izawa, Takato Abe, Naoki Tsukada, Takako Hishiki, Makoto Suematsu, Mayumi Kajimura, Norihiro Suzuki
Oxidative stress plays an important role in the onset and progression of Parkinson disease. Although released dopamine at the synaptic terminal is mostly reabsorbed by dopaminergic neurons, some dopamine is presumably taken up by astroglia. This study examined the dopamine-induced astroglial protective function through the activation of the pentose-phosphate pathway (PPP) to reduce reactive oxygen species (ROS). In vitro experiments were performed using striatal neurons and cortical or striatal astroglia prepared from Sprague-Dawley rats or C57BL/6 mice...
January 2018: ASN Neuro
Camila Ramalho Bonturi, Helena Motaln, Mariana Cristina Cabral Silva, Bruno Ramos Salu, Marlon Vilela de Brito, Luciana de Andrade Luz Cost, Heron Fernandes Vieira Torquato, Natalia Neto Dos Santos Nunes, Edgar Julian Paredes-Gamero, Tamara Lah Turnšek, Maria Luiza Vilela Oliva
Glioblastoma is the most aggressive brain tumor with poor overall survival bellow 2 years. The natural compounds with anti-cancer properties, are thus gaining attention for possible adjuvant GBM treatment. In various cancer models Enterolobium contortisiliquum Trypsin Inhibitor (EcTI) proved to have anti-cancer effects. Here, we investigated the EcTI effects on GBM U87 cells and on mesenchymal stem cells (MSC) compared to their direct coculture (MSC/U87). MSC are present in tumor stroma, modulating GBM cells phenotype, and also represent potential drug delivery vehicle due to their tumor tropism...
April 20, 2018: Oncotarget
Eun Jin Lee, Jiye Hyun, Yong-Ho Choi, Byung-Serk Hurh, Sang-Ho Choi, Inhyung Lee
Doenjang (Korean fermented soybean paste) with an improved flavor and safety was prepared by the simultaneous fermentation of autochthonous mixed starters at the pilot plan scale. First, whole soybean meju was fermented by coculturing safety-verified starters Aspergillus oryzae MJS14 and Bacillus amyloliquefaciens zip6 or Bacillus subtilis D119C. These fermented whole soybean meju were aged in a brine solution after the additional inoculation of Tetragenococcus halophilus 7BDE22 and Zygosaccharomyces rouxii SMY045 to yield doenjang...
May 15, 2018: Journal of Food Science
Byeong-Moo Kim, Ahmed Maher Abdelfattah, Robin Vasan, Bryan C Fuchs, Michael Y Choi
Non-alcoholic fatty liver disease (NAFLD) encompasses a wide spectrum of disease severity, starting from pure steatosis, leading to fatty inflammation labeled as non-alcoholic steatohepatitis (NASH), and finally fibrosis leading to cirrhosis. Activated hepatic stellate cells (HSCs) are known to contribute to fibrosis, but less is known about their function during NAFLD's early stages prior to fibrosis. We developed an ex vivo assay that cocultures primary HSCs from mouse models of liver disease with healthy hepatocytes to study their interaction...
May 14, 2018: Scientific Reports
Kwang Soo Kim, Paul C Marcogliese, Jungwoo Yang, Steve M Callaghan, Virginia Resende, Elizabeth Abdel-Messih, Connie Marras, Naomi P Visanji, Jana Huang, Michael G Schlossmacher, Laura Trinkle-Mulcahy, Ruth S Slack, Anthony E Lang, David S Park
Leucine-rich repeat kinase 2 ( LRRK2 ) has been implicated in both familial and sporadic Parkinson's disease (PD), yet its pathogenic role remains unclear. A previous screen in Drosophila identified Scar/WAVE (Wiskott-Aldrich syndrome protein-family verproline) proteins as potential genetic interactors of LRRK2 Here, we provide evidence that LRRK2 modulates the phagocytic response of myeloid cells via specific modulation of the actin-cytoskeletal regulator, WAVE2. We demonstrate that macrophages and microglia from LRRK2-G2019S PD patients and mice display a WAVE2-mediated increase in phagocytic response, respectively...
May 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
Qing-Ran Bai, Lu Dong, Yi Hao, Xing Chen, Qin Shen
A chemical approach was developed for identifying cell-surface markers for primary neural stem cells (NSCs). Using an in vitro coculture system of primary NSCs combined with metabolic labeling of sialoglycans with bioorthogonal functional groups, we selectively enriched and identified a list of cell-surface sialoglycoproteins that were more abundantly expressed in neural stem and progenitor cells.
May 14, 2018: Chemical Communications: Chem Comm
Ao Jiao, Feng Li, Chengshuo Zhang, Wu Lv, Baomin Chen, Jialin Zhang
Cholinergic neurons can functionally support pancreatic islets in controlling blood sugar levels. However, in islet transplantation, the level of cholinergic reinnervation is significantly lower compared to orthotopic pancreatic islets. This abnormal reinnervation affects the survival and function of islet grafts. In this study, the cholinergic reinnervation of beta cells was simulated by 2D and 3D coculture of INS-1 and NG108-15 cells. In 2D culture conditions, 20 mM glucose induced a 1.24-fold increase ( p < 0...
2018: International Journal of Endocrinology
Matthew J Smith, Matthew B Francis
This work is motivated by both (1) the desire to make interesting products without the reliance on fixed carbon or fixed nitrogen using microbial cocultures, and (2) the desire to develop new methods for growing microbial communities. The bioplastic polyhydroxybutyrate (PHB), for example, is considered prohibitively expensive to make from sugar (compared to polypropylene). Utilizing and building on engineered strains of Synechococcus elongatus and Azotobacter vinelandii, we have combined a nitrogen-fixing organism with a carbon-fixing organism to make PHB from air, water, sunlight, and trace minerals...
2018: Methods in Molecular Biology
Hyeong Min Son, Ha Yeon Kim, Sol Moi Park, Haeng Ki Lee
The present study investigated the CaCO₃ precipitation performance of ureolytic and non-ureolytic bacteria co-cultured as a self-healing agent for cementitious materials crack repair. Three different inoculum ratios of ureolytic Sporosarcina pasteurii and non-ureolytic Bacillus thuringiensis (10:0, 8:2, or 5:5) were used. The effect of coculturing ureolytic and non-ureolytic bacteria on microbial metabolism was investigated by measuring the rate of growth in urea-containing medium and the rate of NH₄⁺ and CaCO₃ production in urea⁻calcium lactate medium...
May 11, 2018: Materials
Pengfei Wei, Zuoying Yuan, Qing Cai, Jianping Mao, Xiaoping Yang
Bone defect repair, especially in terms of infection, remains a big challenge in clinical therapy. To meet clinical requirements, the dual-functional strategy including antibacterial activity and strong osteogenesis effect is a promising approach in bone tissue engineering. In the present study, bioresorbable porous-structured microspheres are fabricated from amphiphilic block copolymer composed of poly(l-lactide) and poly(ethyl glycol) blocks. After being surface coated with mussel-inspired polydopamine, the microspheres are loaded with nanosilver via reduction of silver nitrate and apatite via biomineralization in sequence...
May 11, 2018: Macromolecular Rapid Communications
Jiye Kim, Yoon-Hee Kwon, Chu-Sook Kim, Thai H Tu, Byung-Sam Kim, Yeonsoo Joe, Hun T Chung, Tsuyoshi Goto, Teruo Kawada, Taesun Park, Myung-Sook Choi, Min-Seon Kim, Rina Yu
Obesity-induced inflammation occurs not only in peripheral tissues but also in areas of the central nervous system. Glial cells such as astrocytes and microglia play crucial roles in obesity-related hypothalamic inflammation, leading to the derangement of energy metabolism and neurodegenerative pathologies. Here, we show that the interaction of 4-1BB/4-1BBL between lipid-laden astrocytes/microglia promotes hypothalamic inflammation in obesity. Stimulation of 4-1BB, a member of the TNF receptor superfamily, and/or its ligand 4-1BBL on astrocytes and/or microglia with a specific agonist resulted in activation of the inflammatory signaling pathway and enhanced production of inflammatory mediators...
May 2018: FEBS Open Bio
Anuradha Vaidya, Vaijayanti Kale, Mariyah Poonawala, Suprita Ghode
AIM: To examine the stromal cell-mediated effects of trans-resveratrol (TRV) on the fate of hematopoietic stem cells (HSCs). Materials & methods: Proliferation assay, cell cycle analysis, apoptosis assay, flow cytometry, western blot. RESULTS: Using KG1a, we show that TRV has a dose-dependent effect on the proliferation of hematopoietic cells. Its stimulatory effect was significantly enhanced when the cells were cocultured with stromal cells. Addition of TRV in the coculture of murine bone marrow-derived HSCs and stromal cells led to a significant increase in the pool of long-term HSCs...
May 10, 2018: Regenerative Medicine
M Claireaux, M Galperin, D Benati, A Nouël, M Mukhopadhyay, J Klingler, P de Truchis, D Zucman, S Hendou, F Boufassa, C Moog, O Lambotte, L A Chakrabarti
Follicular helper T cells (Tfh) play an essential role in the affinity maturation of the antibody response by providing help to B cells. To determine whether this CD4+ T cell subset may contribute to the spontaneous control of HIV infection, we analyzed the phenotype and function of circulating Tfh (cTfh) in patients from the ANRS CO21 CODEX cohort who naturally controlled HIV-1 replication to undetectable levels and compared them to treated patients with similarly low viral loads. HIV-specific cTfh (Tet+ ), detected by Gag-major histocompatibility complex class II (MHC-II) tetramer labeling in the CD45RA- CXCR5+ CD4+ T cell population, proved more frequent in the controller group ( P = 0...
May 8, 2018: MBio
Thomas Dixon, Eliad Cohen, Dana Meredith Cairns, Maria Rodriguez, Juanita Mathews, Rod Jose, David L Kaplan
The physical connection between motoneurons and skeletal muscle targets is responsible for the creation of neuromuscular junctions (NMJs), which allow electrical signals to be translated to mechanical work. NMJ pathology contributes to the spectrum of neuromuscular, motoneuron, and dystrophic disease. Improving in vitro tools that allow for recapitulation of the physiology of the neuromuscular connection will enable researchers to better understand the development and maturation of NMJs, and will help to decipher mechanisms leading to NMJ degeneration...
May 9, 2018: Tissue Engineering. Part C, Methods
Chao Li, Jiaquan Yu, Jennifer Schehr, Scott M Berry, Ticiana A Leal, Joshua M Lang, David J Beebe
The concept of high liquid repellency in multi-liquid-phase systems (e.g., aqueous droplets in an oil background) has been applied to areas of biomedical research to realize intrinsic advantages not available in single-liquid-phase systems. Such advantages have included minimizing analyte loss, facile manipulation of single-cell samples, elimination of biofouling, and ease of use regarding loading and retrieving of the sample. In this paper, we present generalized design rules for predicting the wettability of solid-liquid-liquid systems (especially for discrimination between exclusive liquid repellency (ELR) and finite liquid repellency) to extend the applications of ELR...
May 8, 2018: ACS Applied Materials & Interfaces
Danielle E Jondal, Scott M Thompson, Kim A Butters, Bruce E Knudsen, Jill L Anderson, Rickey E Carter, Lewis R Roberts, Matthew R Callstrom, David A Woodrum
Purpose To determine if heat stress and hepatic laser thermal ablation induce hepatocellular carcinoma (HCC) growth and to identify growth factors induced by heat stress. Materials and Methods Non-heat-stressed HCC cells were cocultured with HCC cells or hepatocytes that were heat stressed at 37°C (physiologic), 45°C (moderate), or 50°C (severe) for 10 minutes and proliferation monitored with bioluminescence imaging for up to 6 days after heat stress (three experiments). Rats bearing orthotopic N1S1 HCC were randomly assigned to undergo immediate sham or laser thermal (3 W for 60 or 90 seconds; hereafter, 3W×60s and 3W×90s, respectively) ablation of the median (local) or left (distant) hepatic lobe, and tumor growth was monitored with magnetic resonance imaging for up to 18 days after ablation (six or more rats per group)...
May 8, 2018: Radiology
Shuling Hu, Jeonghyun Park, Airan Liu, JaeHoon Lee, Xiwen Zhang, Qi Hao, Jae-Woo Lee
Our previous study demonstrated that mesenchymal stem cell (MSC) microvesicles (MV) reduced lung inflammation, protein permeability, and pulmonary edema in endotoxin-induced acute lung injury in mice. However, the underlying mechanisms for restoring lung protein permeability were not fully understood. In this current study, we hypothesized that MSC MV would restore protein permeability across injured human lung microvascular endothelial cells (HLMVEC) in part through the transfer of angiopoietin-1 (Ang1) mRNA to the injured endothelium...
May 8, 2018: Stem Cells Translational Medicine
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