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https://www.readbyqxmd.com/read/29139088/contrasting-views-on-the-role-of-mesenchymal-stromal-stem-cells-in-tumour-growth-a-systematic-review-of-experimental-design
#1
Ahmed Kolade Oloyo, Melvin Anyasi Ambele, Michael Sean Pepper
The effect of mesenchymal stromal/stem cells (MSCs) on tumour growth remains controversial. Experimental evidence supports both an inhibitory and a stimulatory effect. We have assessed factors responsible for the contrasting effects of MSCs on tumour growth by doing a meta-analysis of existing literature between 2000 and May 2017. We assessed 183 original research articles comprising 338 experiments. We considered (a) in vivo and in vitro experiments, (b) whether in vivo studies were syngeneic or xenogeneic, and (c) if animals were immune competent or deficient...
November 15, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29137981/umbilical-cord-derived-mesenchymal-stem-cells-alleviated-inflammation-and-inhibited-apoptosis-in-interstitial-cystitis-via-akt-mtor-signaling-pathway
#2
Juncong Xie, Bolong Liu, Jialiang Chen, Yuancheng Xu, Hailun Zhan, Fei Yang, Wenbiao Li, Xiangfu Zhou
Interstitial cystitis (IC) is a bladder syndrome characterized by pelvic pain and urinary frequency without infection or other identifiable pathology. There are no effective treatments to cure IC. This study investigated the effects of human umbilical cord-derived mesenchymal stem cells (UC-MSCs) injection on IC rat model. Furthermore, we used a coculture system to find the possible molecular mechanism on the human uroepithelial cells (SV-HUC-1), which was the cell model of IC. A rat model of IC was established via systemic injection with cyclophosphamide (CYP) and a cell model of IC was induced by being exposed to tumor necrosis factor (TNF)-α (10 ng/ml)...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137978/endothelial-robo4-regulates-il-6-production-by-endothelial-cells-and-monocytes-via-a-crosstalk-mechanism-in-inflammation
#3
Keisuke Shirakura, Ryosuke Ishiba, Taito Kashio, Miki Sakai, Yu Fukushima, Nana Yamamoto, Shiori Manabe, Naoya Shigesada, Toru Tanaka, Nobumasa Hino, William C Aird, Takefumi Doi, Yoshiaki Okada
Roundabout4 (Robo4) is an endothelial cell-specific receptor that stabilizes vasculature in pathological angiogenesis. Previous studies have shown that Robo4 is a potential therapeutic target for inflammatory diseases, but its precise roles in inflammation remain unclear. To investigate physiological Robo4 functions in inflammation, we performed a loss-of-function study in vitro and in vivo using lipopolysaccharide (LPS)-induced endotoxemia models. Subcutaneous injection of LPS into Robo4-knockout mice reduced circulating IL-6 levels...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29135052/enhanced-effect-of-human-mesenchymal-stem-cells-expressing-human-tnf-%C3%AE-r-fc-and-ho-1-gene-on-porcine-islet-xenotransplantation-in-humanized-mice
#4
Han-Sin Lee, Sanghyun Song, Du Yeon Shin, Geun-Soo Kim, Jong-Hyun Lee, Chan Woo Cho, Kyo Won Lee, Hyojun Park, Curie Ahn, Jaeseok Yang, Heung-Mo Yang, Jae Berm Park, Sung-Joo Kim
BACKGROUND: Porcine islet xenotransplantation is considered an attractive alternative treatment for type 1 diabetes mellitus. However, it is largely limited because of initial rejection due to Instant Blood-Mediated Inflammatory Reaction (IBMIR), oxidative stress, and inflammatory responses. Recently, soluble tumor necrosis factor-ɑ receptor type I (sTNF-αR) and heme oxygenase (HO)-1 genes (HO-1/sTNF-αR) have been shown to improve the viability and functionality of porcine islets after transplantation...
November 14, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29132013/chimeric-antigen-receptor-modified-t-cells-redirected-to-epha2-for-the-immunotherapy-of-non-small-cell-lung-cancer
#5
Ning Li, Shaohui Liu, Mingjiao Sun, Wei Chen, Xiaogang Xu, Zhu Zeng, Yemin Tang, Yongquan Dong, Alex H Chang, Qiong Zhao
Erythropoietin-producing hepatocellular carcinoma A2 (EphA2) is overexpressed in more than 90% of non-small cell lung cancer (NSCLC) but not significantly in normal lung tissue. It is therefore an important tumor antigen target for chimeric antigen receptors (CAR)-T-based therapy in NSCLC. Here, we developed a specific CAR targeted to EphA2, and the anti-tumor effects of this CAR were investigated. A second generation CAR with co-stimulatory receptor 4-1BB targeted to EphA2 was developed. The functionality of EphA2-specific T cells in vitro was tested with flow cytometry and real-time cell electronic sensing system assays...
November 10, 2017: Translational Oncology
https://www.readbyqxmd.com/read/29130200/padlock-probes-to-detect-single-nucleotide-polymorphisms
#6
Tomasz Krzywkowski, Mats Nilsson
Rapid development of high-throughput DNA analyzation methods has enabled global characterization of genetic landscapes and aberrations in study subjects in a time and cost effective fashion. In most methods, however, spatial tissue context is lost since sample preparation requires isolation of nucleic acids out of their native environment. We hereby present the most recent protocol for multiplexed, in situ detection of mRNAs and single nucleotide polymorphisms using padlock probes and rolling circle amplification...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130181/a-coculture-based-approach-for-screening-campaigns-aimed-at-identifying-novel-pseudomonas-aeruginosa-quorum-sensing-inhibitors
#7
Giordano Rampioni, Giulia Giallonardi, Francesca D'Angelo, Livia Leoni
Quorum sensing (QS) is recognized as a promising target for the identification of anti-virulence drugs hampering Pseudomonas aeruginosa adaptability to the host environment and pathogenicity. Consequently, a number of studies in the last decade focused on the identification of small molecules or proteins with anti-QS activity, mainly targeting the las QS system, which is based on N-3-oxododecanoyl-homoserine lactone (3OC12-HSL) as signal molecule. Different experimental approaches have been successfully used to identify QS blockers interfering with the activity/stability of the 3OC12-HSL receptor LasR, with the functionality of the 3OC12-HSL synthase LasI, or with the stability/bioavailability of the 3OC12-HSL signal molecule itself...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29129566/pediatric-glioblastoma-cells-inhibit-neurogenesis-and-promote-astrogenesis-phenotypic-transformation-and-migration-of-human-neural-progenitor-cells-within-cocultures
#8
Kurt Farrell, Gautam Mahajan, Parthasarathy Srinivasan, Moo-Yeal Lee, Chandrasekhar R Kothapalli
Neural progenitor cell (NPC) fate is influenced by a variety of biological cues elicited from the surrounding microenvironment and recent studies suggest their possible role in pediatric glioblastoma multiforme (GBM) development. Since a few GBM cells also display NPC characteristics, it is not clear whether NPCs transform to tumor cell phenotype leading to the onset of GBM formation, or NPCs migrate to developing tumor sites in response to paracrine signaling from GBM cells. Elucidating the paracrine interactions between GBM cells and NPCs in vivo is challenging due to the inherent complexity of the CNS...
November 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29126441/tgf%C3%AE-3-recruits-endogenous-mesenchymal-stem-cells-to-initiate-bone-regeneration
#9
Moyuan Deng, Tieniu Mei, Tianyong Hou, Keyu Luo, Fei Luo, Aijun Yang, Bo Yu, Hao Pang, Shiwu Dong, Jianzhong Xu
BACKGROUND: The recruitment of a sufficient number of endogenous mesenchymal stem cells (MSCs) is the first stage of in-situ tissue regeneration. Transforming growth factor beta-3 (TGFβ3) could recruit stem or progenitor cells and endothelial cells to participate in tissue regeneration. However, the mechanism of TGFβ3 recruiting MSCs toward bone regeneration has remained obscure. METHODS: We estimated the promigratory property of TGFβ3 on human bone marrow MSCs (hBMSCs) cocultured with the vascular cells (human umbilical artery smooth muscle cells or human umbilical vein endothelial cells) or not by Transwell assay...
November 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29123981/coculture-of-endothelial-progenitor-cells-and-mesenchymal-stem-cells-enhanced-their-proliferation-and-angiogenesis-through-pdgf-and-notch-signaling
#10
Tangzhao Liang, Lei Zhu, Wenling Gao, Ming Gong, Jianhua Ren, Hui Yao, Kun Wang, Dehai Shi
The beneficial effects of combined use of mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) on tissue repair and regeneration after injury have been demonstrated, but the underlying mechanism remains incompletely understood. This study aimed to investigate the effects of direct contact coculture of human bone marrow-derived EPCs (hEPCs)/human bone marrow-derived MSCs (hMSCs) on their proliferation and angiogenic capacities and the underlying mechanism. hEPCs and hMSCs were cocultured in a 2D mixed monolayer or a 3D transwell membrane cell-to-cell coculture system...
November 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29123469/suppression-of-inflammatory-demyelinaton-and-axon-degeneration-through-inhibiting-kv3-channels
#11
Peter Jukkola, Yuanzheng Gu, Amy E Lovett-Racke, Chen Gu
The development of neuroprotective and repair strategies for treating progressive multiple sclerosis (MS) requires new insights into axonal injury. 4-aminopyridine (4-AP), a blocker of voltage-gated K(+) (Kv) channels, is used in symptomatic treatment of progressive MS, but the underlying mechanism remains unclear. Here we report that deleting Kv3.1-the channel with the highest 4-AP sensitivity-reduces clinical signs in experimental autoimmune encephalomyelitis (EAE), a mouse model for MS. In Kv3.1 knockout (KO) mice, EAE lesions in sensory and motor tracts of spinal cord were markedly reduced, and radial astroglia were activated with increased expression of brain derived neurotrophic factor (BDNF)...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29121426/xanthohumol-inhibits-angiogenesis-by-suppressing-nuclear-factor-%C3%AE%C2%BAb-activation-in-pancreatic-cancer
#12
Kenta Saito, Yoichi Matsuo, Hiroyuki Imafuji, Tomotaka Okubo, Yuzo Maeda, Takafumi Sato, Tomoya Shamoto, Ken Tsuboi, Mamoru Morimoto, Hiroki Takahashi, Hideyuki Ishiguro, Shuji Takiguchi
Xantohumol, a prenylated chalcone from hops (Humulus lupulus L.), has been shown to inhibit proliferation in some cancers. However, little is known regarding the effects of xanthohumol in pancreatic cancer. We have previously reported that activation of the transcription factor nuclear factor-κB (NF-κB) plays a key role in angiogenesis in pancreatic cancer. In this study, we investigated whether xanthohumol inhibited angiogenesis by blocking NF-κB activation in pancreatic cancer in vitro and in vivo. We initially confirmed that xanthohumol significantly inhibited proliferation and NF-κB activation in pancreatic cancer cell lines...
November 9, 2017: Cancer Science
https://www.readbyqxmd.com/read/29119119/an-exploration-of-the-impact-of-anticentromere-antibody-on-early-stage-embryo
#13
Ying Ying, Xi Guo, Yiping Zhong, Canquan Zhou
Background: Previously, we found women with positive anticentromere antibody showed impaired potential of oocyte maturation and embryo cleavage; the possible mechanism behind this phenomenon was still unknown. Objective: Thus, the present study aimed to preliminarily explore whether ACA could penetrate into the living embryos and impair their developmental potential via in vitro coculture with mouse embryos. Methods: Mouse embryos were collected and used for in vitro culture with polyclonal anticentromere protein A (CENP-A) antibody; then, immunofluorescence assay was performed to determine the penetration of antibody into embryos, and embryo development potential was observed...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/29116998/mesenchymal-stem-cells-from-bone-marrow-adipose-tissue-and-lung-tissue-differentially-mitigate-lung-and-distal-organ-damage-in-experimental-acute-respiratory-distress-syndrome
#14
Johnatas D Silva, Miquéias Lopes-Pacheco, Ana H R Paz, Fernanda F Cruz, Elga B Melo, Milena V de Oliveira, Débora G Xisto, Vera L Capelozzi, Marcelo M Morales, Paolo Pelosi, Elizabeth Cirne-Lima, Patricia R M Rocco
OBJECTIVES: Mesenchymal stem cells-based therapies have shown promising effects in experimental acute respiratory distress syndrome. Different mesenchymal stem cells sources may result in diverse effects in respiratory diseases; however, there is no information regarding the best source of mesenchymal stem cells to treat pulmonary acute respiratory distress syndrome. We tested the hypothesis that mesenchymal stem cells derived from bone marrow, adipose tissue, and lung tissue would lead to different beneficial effects on lung and distal organ damage in experimental pulmonary acute respiratory distress syndrome...
November 7, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/29115987/cancer-cell-soluble-factors-reprogram-mesenchymal-stromal-cells-to-slow-cycling-chemoresistant-cells-with-a-more-stem-like-state
#15
Ahmed El-Badawy, Mohamed A Ghoneim, Mahmoud M Gabr, Radwa Ayman Salah, Ihab K Mohamed, Marwa Amer, Nagwa El-Badri
BACKGROUND: Mesenchymal stem cells (MSCs) play different roles in modulating tumor progression, growth, and metastasis. MSCs are recruited to the tumor site in large numbers and subsequently have an important microenvironmental role in modulating tumor progression and drug sensitivity. However, the effect of the tumor microenvironment on MSC plasticity remains poorly understood. Herein, we report a paracrine effect of cancer cells, in which they secrete soluble factors that promote a more stem-like state in bone marrow mesenchymal stem cells (BM-MSCs)...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29115054/comparison-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-with-healthy-fibroblasts-on-wound-healing-activity-of-diabetic-fibroblasts
#16
Jae-A Jung, Young-Don Yoon, Hyup-Woo Lee, So-Ra Kang, Seung-Kyu Han
Various types of skin substitutes composed of fibroblasts and/or keratinocytes have been used for the treatment of diabetic ulcers. However, the effects have generally not been very dramatic. Recently, human umbilical cord blood-derived mesenchymal stromal cells (hUCB-MSCs) have been commercialised for cartilage repair as a first cell therapy product using allogeneic stem cells. In a previous pilot study, we reported that hUCB-MSCs have a superior wound-healing capability compared with fibroblasts. The present study was designed to compare the treatment effect of hUCB-MSCs with that of fibroblasts on the diabetic wound healing in vitro...
November 7, 2017: International Wound Journal
https://www.readbyqxmd.com/read/29114031/different-substrate-preferences-help-closely-related-bacteria-to-coexist-in-the-gut
#17
Petra Louis
Many factors shape the ability of different microbes to coexist in microbial communities. In the human gut, dietary and host-derived nutrients largely drive microbial community structure. How gut microbes with very similar nutrient profiles are able to coexist over time within the same host is not fully understood. Tuncil et al. (mBio 8:e01068-17, 2017, https://doi.org/10.1128/mBio.01068-17) explored glycan prioritization in two closely related human gut bacteria, Bacteroides ovatus and Bacteroides thetaiotaomicron, on complex glycan mixtures that both organisms can degrade...
November 7, 2017: MBio
https://www.readbyqxmd.com/read/29111993/enhanced-osteogenic-differentiation-potential-of-stem-cell-spheroids-created-from-a-coculture-of-stem-cells-and-endothelial-cells
#18
Jae-Yong Tae, Sung-Il Lee, Youngkyung Ko, Jun-Beom Park
PURPOSE: This study was performed to fabricate stem-cell spheroids formed with human gingiva-derived stem cells and endothelial cells and to evaluate their viability and osteogenic differentiation potential. MATERIALS AND METHODS: Gingiva-derived stem cells were isolated, and stem cells and endothelial cells with a total of 6 × 10 cells were seeded into concave micromolds with different ratios of 6:0 (group 1), 4:2 (group 2), 3:3 (group 3), and 2:4 (group 4). RESULTS: Gingiva-derived stem cells and/or endothelia cells formed spheroids in concave microwells...
November 2, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/29104466/the-crosstalk-between-hdpscs-and-hucmscs-on-proliferation-and-osteogenic-genes-expression-in-coculture-system
#19
Linglu Jia, Weiting Gu, Yunpeng Zhang, Yawen Ji, Jin Liang, Yong Wen, Xin Xu
Objectives: The present study established a non-contact coculture system in vitro, aiming to investigate the crosstalk between human dental pulp stem cells (hDPSCs) and human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation activity and osteogenic genes expression through paracrine. Materials and methods: The stemness of hDPSCs and hUCMSCs were identified by flow cytometric analysis and multipotential differentiation assays. With the help of transwell inserts, the non-contact coculture system in vitro was established between hDPSCs and hUCMSCs...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29102766/facilitating-effects-of-buyang-huanwu-decoction-on-axonal-regeneration-after-peripheral-nerve-transection
#20
Ki Joong Kim, Uk Namgung
ETHNOPHARMACOLOGICAL RELEVANCE: In traditional Asian medicine, Buyang Huanwu decoction (BYHWD) has been used for the treatment of cardiovascular and neurological disorders. Recent experimental studies have begun to provide evidence on the protective effects of BYHWD on injured peripheral nerves. AIM OF THE STUDY: To examine whether BYHWD was effective in inducing axonal regeneration after peripheral nerve transection, and if so, how it acted on the nerve. MATERIALS AND METHODS: The sciatic nerve in rats was transected and resutured 0, 1, or 4 weeks later...
November 1, 2017: Journal of Ethnopharmacology
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