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https://www.readbyqxmd.com/read/29758548/stromal-cell-derived-factor-sdf-1-alpha-overexpression-promotes-bone-regeneration-by-osteogenesis-and-angiogenesis-in-osteonecrosis-of-the-femoral-head
#1
Fan Yang, Feng Xue, Junjie Guan, Zeng Zhang, Jimin Yin, Qingling Kang
BACKGROUND/AIMS: Osteonecrosis of the femoral head (ONFH) is a devastating orthopedic disease. Previous studies suggested that stromal-cell-derived factor (SDF)-1 was involved in osteogenesis and angiogenesis. However, whether SDF-1 potentiates the angiogenesis and osteogenesis of bone marrow-derived stromal stem cells (BMSCs) in ONFH is not clear. METHODS: BMSCs were transfected with green fluorescent protein (GFP) or the fusion gene encoding GFP and SDF-1α, and transgenic efficacy was monitored by immunofluorescence...
May 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29751359/bone-morphogenetic-protein-9-as-a-key-regulator-of-liver-progenitor-cells-in-ddc-induced-cholestatic-liver-injury
#2
Annalisa Addante, Cesáreo Roncero, Laura Almalé, Nerea Lazcanoiturburu, María García-Álvaro, Margarita Fernández, Julián Sanz, Seddik Hammad, Zeribe Chike Nwosu, Se-Jin Lee, Isabel Fabregat, Steven Dooley, Peter Ten Dijke, Blanca Herrera, Aránzazu Sánchez
BACKGROUND & AIMS: Bone morphogenetic protein 9 (BMP9) interferes with liver regeneration upon acute injury, while promoting fibrosis upon carbon tetrachloride-induced chronic injury. We have now addressed the role of BMP9 in 3,5 diethoxicarbonyl-1,4 dihydrocollidine (DDC)-induced cholestatic liver injury, a model of liver regeneration mediated by hepatic progenitor cell (known as oval cell), exemplified as ductular reaction and oval cell expansion. METHODS: WT and BMP9KO mice were submitted to DDC diet...
May 11, 2018: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/29750278/bmp6-down-regulates-gdnf-expression-through-smad1-5-and-erk1-2-signaling-pathways-in-human-granulosa-lutein-cells
#3
Xin-Yue Zhang, Hsun-Ming Chang, Elizabeth L Taylor, Peter C K Leung, Rui-Zhi Liu
Bone morphogenetic protein 6 (BMP6) is a critical regulator of follicular development that is expressed in mammalian oocytes and granulosa cells. Glial cell line-derived neurotrophic factor (GDNF) is an intraovarian neurotrophic factor that plays an essential role in regulating mammalian oocyte maturation. The aim of this study was to investigate the effect of BMP6 on the regulation of GDNF expression and the potential underlying mechanisms. We used an established immortalized human granulosa cell line (SVOG cells) and primary human granulosa-lutein cells as in vitro cell models...
May 9, 2018: Endocrinology
https://www.readbyqxmd.com/read/29739878/bmpr2-inhibits-activin-and-bmp-signaling-via-wild-type-alk2
#4
Oddrun Elise Olsen, Meenu Sankar, Samah Elsaadi, Hanne Hella, Glenn Buene, Sagar Ramesh Darvekar, Kristine Misund, Takenobu Katagiri, Petra Knaus, Toril Holien
TGF-β/BMP superfamily ligands require heteromeric complexes of type 1 and 2 receptors for ligand dependent downstream signaling. Activin A, a TGF-β superfamily member, inhibits growth of multiple myeloma cells, but the mechanism is unknown. We aimed to clarify how activins affect myeloma cell survival. Activin A activates the transcription factors SMAD2/3 through the ALK4 type 1 receptor, but may also activate SMAD1/5/8 through mutated variants of the type 1 receptor ALK2. We demonstrate that activin A and B activate SMAD1/5/8 in myeloma cells through endogenous wild type ALK2...
May 8, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29736214/masquelet-s-induced-membrane-promotes-the-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-by-activating-the-smad-and-mapk-pathways
#5
Qian Tang, Minji Tong, Gang Zheng, Liyan Shen, Ping Shang, Haixiao Liu
The Masquelet's induced membrane (IM) technique is widely used to treat large segmental bone defects due to its physical priority and biological function. However, the underlying molecular mechanism of the IM on bone formation remains unknown. In the present study, rat bone marrow-derived mesenchymal stem cells (BMSCs) were used as an in vitro model and bone morphogenetic protein 2 (BMP-2) was used as a positive control to evaluate the effects of the IM on the osteogenic differentiation of BMSCs. Although the IM group did not exhibit a significant increase in the expression of Runt-related transcription factor 2 (Runx2), Collagen I (Col I), osteocalcin (OCN) and alkaline phosphatase (ALP) relative to the BMP-2 administration, the IM was considerably effective compared with the untreated group...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29729706/tgf%C3%AE-1-mediated-pi3k-akt-and-p38-map-kinase-dependent-alternative-splicing-of-fibronectin-extra-domain-a-in-human-podocyte-culture
#6
Tarunkumar Hemraj Madne, Mark Edward Carl Dockrell
Alternative splicing is an important gene regulation process to distribute proteins in health and diseases. Extra Domain A+ Fibronectin (EDA+Fn) is an alternatively spliced form of fibronectin (Fn) protein, present in the extra cellular matrix (ECM) and a recognised marker of various pathologies. TGFβ1 has been shown to induce alternative splicing of EDA+Fn in many cell types. Podocytes are spectacular cell type and play a key role in filtration and synthesise ECM proteins in renal physiology and pathology...
April 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29728612/identification-of-the-effector-domain-of-biglycan-that-facilitates-bmp-2-osteogenic-function
#7
Prapaporn Jongwattanapisan, Masahiko Terajima, Patricia A Miguez, William Querido, Hideaki Nagaoka, Noriko Sumida, Elizabeth Grace Gurysh, Kristy M Ainslie, Nancy Pleshko, Lalith Perera, Mitsuo Yamauchi
We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain ("effector") within the BGN core protein that facilitates BMP-2 osteogenic function. Thus, we generated various recombinant and synthetic peptides corresponding to several domains of BGN, and tested their effects on BMP-2 functions in vitro. The results demonstrated that the leucine-rich repeats 2-3 domain (LRR2-3) of BGN significantly enhanced the BMP-2 induced Smad1/5/9 phosphorylation, osteogenic gene expression, and alkaline phosphatase activity in myogenic C2C12 cells...
May 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29715457/spg6-supports-development-of-acute-myeloid-leukemia-by-regulating-bmpr2-smad-bcl-2-bcl-xl-signaling
#8
Jinliang Chen, Chunling Li, Renhui Zhan, Yancun Yin
Acute myeloid leukemia (AML) is the most common acute leukemia affecting adults. To effectively treat AML, new molecular targets and therapeutic approaches must be identified. In silico analysis of several available databases of AML patients showed that the expression of Spastic Paraplegia 6 Protein (SPG6) significantly inversely correlates with the overall survival of AML patients. To determine whether SPG6 supports AML development, we employed an shRNA-encoding lentivirus system to inhibit SPG6 expression in human AML cells including NB4 and MV4-11 cells...
April 28, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29700355/claudin-11-regulates-bone-homeostasis-via-bidirectional-ephb4-ephrinb2-signaling
#9
Jong Min Baek, Yoon-Hee Cheon, Sung Chul Kwak, Hong Young Jun, Kwon-Ha Yoon, Myeung Su Lee, Ju-Young Kim
Claudins (Cldns) are well-established components of tight junctions (TJs) that play a pivotal role in the modulation of paracellular permeability. Several studies have explored the physiologic aspects of Cldn family members in bone metabolism. However, the effect of Cldn11, a major component of central nervous system myelin, on bone homeostasis has not been reported. In this study, we demonstrate that Cldn11 is a potential target for bone disease therapeutics as a dual modulator of osteogenesis enhancement and osteoclastogenesis inhibition...
April 27, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29686106/embryonic-regeneration-by-relocalization-of-the-spemann-organizer-during-twinning-in-xenopus
#10
Yuki Moriyama, Edward M De Robertis
The formation of identical twins from a single egg has fascinated developmental biologists for a very long time. Previous work had shown that Xenopus blastulae bisected along the dorsal-ventral (D-V) midline (i.e., the sagittal plane) could generate twins but at very low frequencies. Here, we have improved this method by using an eyelash knife and changing saline solutions, reaching frequencies of twinning of 50% or more. This allowed mechanistic analysis of the twinning process. We unexpectedly observed that the epidermis of the resulting twins was asymmetrically pigmented at the tailbud stage of regenerating tadpoles...
April 23, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29677202/%C3%AE-1-integrins-mediate-the-bmp2-dependent-transcriptional-control-of-osteoblast-differentiation-and-osteogenesis
#11
Molly Brunner, Noémie Mandier, Thierry Gautier, Genevieve Chevalier, Anne-Sophie Ribba, Philippe Guardiola, Marc R Block, Daniel Bouvard
Osteoblast differentiation is a highly regulated process that requires coordinated information from both soluble factors and the extracellular matrix. Among these extracellular stimuli, chemical and physical properties of the matrix are sensed through cell surface receptors such as integrins and transmitted into the nucleus to drive specific gene expression. Here, we showed that the conditional deletion of β1 integrins in the osteo-precursor population severely impacts bone formation and homeostasis both in vivo and in vitro...
2018: PloS One
https://www.readbyqxmd.com/read/29664133/coordinated-regulation-of-the-dorsal-ventral-and-anterior-posterior-patterning-of-xenopus-embryos-by-the-btb-poz-zinc-finger-protein-zbtb14
#12
Kimiko Takebayashi-Suzuki, Hidenori Konishi, Tatsuo Miyamoto, Tomoko Nagata, Misa Uchida, Atsushi Suzuki
During early vertebrate embryogenesis, bone morphogenetic proteins (BMPs) belonging to the transforming growth factor-β (TGF-β) family of growth factors play a central role in dorsal-ventral (DV) patterning of embryos, while other growth factors such as Wnt and fibroblast growth factor (FGF) family members regulate formation of the anterior-posterior (AP) axis. Although the establishment of body plan is thought to require coordinated formation of the DV and AP axes, the mechanistic details underlying this coordination are not well understood...
April 2018: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/29663368/a-peptide-that-blocks-the-interaction-of-nf-%C3%AE%C2%BAb-p65-subunit-with-smad4-enhances-bmp2-induced-osteogenesis
#13
Mariko Urata, Shoichiro Kokabu, Takuma Matsubara, Goro Sugiyama, Chihiro Nakatomi, Hiroshi Takeuchi, Shizu Hirata-Tsuchiya, Kazuhiro Aoki, Yukihiko Tamura, Yasuko Moriyama, Yasunori Ayukawa, Miho Matsuda, Min Zhang, Kiyoshi Koyano, Chiaki Kitamura, Eijiro Jimi
Bone morphogenetic protein (BMP) potentiates bone formation through the Smad signaling pathway in vitro and in vivo. The transcription factor nuclear factor κB (NF-κB) suppresses BMP-induced osteoblast differentiation. Recently, we identified that the transactivation (TA) 2 domain of p65, a main subunit of NF-κB, interacts with the mad homology (MH) 1 domain of Smad4 to inhibit BMP signaling. Therefore, we further attempted to identify the interacting regions of these two molecules at the amino acid level...
April 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29587293/effects-of-mir-375-bmpr2-as-a-key-factor-downstream-of-bmp15-gdf9-on-the-smad1-5-8-and-smad2-3-signaling-pathways
#14
Chang Liu, Bao Yuan, Hongyan Chen, Mingqiang Xu, Xulei Sun, JiaJun Xu, Yan Gao, Chengzhen Chen, Hao Jiang, Jiabao Zhang
BACKGROUND/AIMS: Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9), which are secreted by oocytes, are important regulators of follicular growth and development and ovarian function. These two factors can regulate the proliferation and apoptosis of cumulus cells via modulation of the Smad signaling pathway. Studies have shown that BMP15 and GDF9 can affect the level of miR-375, whereas the target gene of miR-375 is BMPR2, the type II receptor of BMP15 and GDF9...
March 21, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29581184/loss-of-the-hematopoietic-stem-cell-factor-gata2-in-the-osteogenic-lineage-impairs-trabecularization-and-mechanical-strength-of-bone
#15
Alexander Tolkachov, Cornelius Fischer, Thomas H Ambrosi, Melissa Bothe, Chung-Ting Han, Matthias Muenzner, Susanne Mathia, Marjo Salminen, Georg Seifert, Mario Thiele, Georg N Duda, Sebastiaan H Meijsing, Sascha Sauer, Tim J Schulz, Michael Schupp
The transcription factor GATA2 is required for expansion and differentiation of hematopoietic stem cells (HSCs). In mesenchymal stem cells (MSCs) GATA2 blocks adipogenesis, but its biological relevance and underlying genomic events are unknown. We report a dual function of GATA2 in bone homeostasis. GATA2 in MSCs binds near genes involved in skeletal system development and co-localizes with motifs for FOX and HOX transcription factors, known regulators of skeletal development. Ectopic GATA2 blocks osteoblastogenesis by interfering with SMAD1/5/8 activation...
March 26, 2018: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29575577/smad7-deficiency-decreases-iron-and-haemoglobin-through-hepcidin-up-regulation-by-multilayer-compensatory-mechanisms
#16
Peng An, Hao Wang, Qian Wu, Jiaming Wang, Zhidan Xia, Xuyan He, Xinhui Wang, Yan Chen, Junxia Min, Fudi Wang
To maintain iron homoeostasis, the iron regulatory hormone hepcidin is tightly controlled by BMP-Smad signalling pathway, but the physiological role of Smad7 in hepcidin regulation remains elusive. We generated and characterized hepatocyte-specific Smad7 knockout mice (Smad7Alb/Alb ), which showed decreased serum iron, tissue iron, haemoglobin concentration, up-regulated hepcidin and increased phosphor-Smad1/5/8 levels in both isolated primary hepatocytes and liver tissues. Increased levels of hepcidin lead to reduced expression of intestinal ferroportin and mild iron deficiency anaemia...
March 25, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29567599/vitamin-c-linker-conjugated-tripeptide-ahk-stimulates-bmp-2-induced-osteogenic-differentiation-of-mouse-myoblast-c2c12-cells
#17
Jung-Il Jung, Kyeong-Yong Park, Yura Lee, Mira Park, Jiyeon Kim
Vitamin C-linker-conjugated Ala-His-Lys tripeptide (Vit C-AHK) is a derivative of Vitamin C-conjugated tripeptides, which were originally developed as a component of a product for collagen synthesis enhancement or human dermal fibroblast growth. Here, we investigated the effect of Vit C-AHK on bone morphogenetic protein (BMP)-2-induced osteoblast differentiation in a cell culture model. Vit C-AHK enhanced proliferation of C2C12 cells and induction of BMP-2-induced alkaline phosphatase, a typical marker of osteoblast differentiation...
March 15, 2018: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29563538/ucma-grp-inhibits-phosphate-induced-vascular-smooth-muscle-cell-calcification-via-smad-dependent-bmp-signalling
#18
Brecht A Willems, Malgorzata Furmanik, Marjolein M J Caron, Martijn L L Chatrou, Dennis H M Kusters, Tim J M Welting, Michael Stock, Marta S Rafael, Carla S B Viegas, Dina C Simes, Cees Vermeer, Chris P M Reutelingsperger, Leon J Schurgers
Vascular calcification (VC) is the process of deposition of calcium phosphate crystals in the blood vessel wall, with a central role for vascular smooth muscle cells (VSMCs). VC is highly prevalent in chronic kidney disease (CKD) patients and thought, in part, to be induced by phosphate imbalance. The molecular mechanisms that regulate VC are not fully known. Here we propose a novel role for the mineralisation regulator Ucma/GRP (Upper zone of growth plate and Cartilage Matrix Associated protein/Gla Rich Protein) in phosphate-induced VSMC calcification...
March 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29560558/in-vitro-evaluation-of-the-potential-therapeutic-role-of-dendropanax-morbifera-extract-in-ameliorating-osteoporosis-and-resultant-bone-impairment-using-mc3t3-e1-cells
#19
Dong-Uk Yang, Muhammad Hanif Siddiqi, Sungeun Ahn, Sera Kang, Hae-Yong Noh, Deok-Chun Yang
Osteoporosis is a widespread musculoskeletal deformity that affects thousands of older people every year, leading to bone abnormalities and ultimately increasing the risk of bone fractures in both genders. It is considered a lethal disease causing death in thousands of people at the late stage of life. Dendropanax morbifera Leveille is a subtropical broad-leaved prevalent species in Korea. Extracts of the leaves, stems, roots, and seeds of D. morbifera have been used in traditional medicine for the treatment of numerous diseases such as diabetes, atherogenesis, skin disorders, and headaches...
May 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29556203/myd88-adaptor-protein-is-required-for-appropriate-hepcidin-induction-in-response-to-dietary-iron-overload-in-mice
#20
Antonio Layoun, Macha Samba-Mondonga, Gabriela Fragoso, Annie Calvé, Manuela M Santos
Iron homeostasis is tightly regulated to provide virtually all cells in the body, particularly red blood cells, with this essential element while defending against its toxicity. The peptide hormone hepcidin is central to the control of the amount of iron absorbed from the diet and iron recycling from macrophages. Previously, we have shown that hepcidin induction in macrophages following Toll-like receptor (TLR) stimulation depends on the presence of myeloid differentiation primary response gene 88 (MyD88). In this study, we analyzed the regulation of iron metabolism in MyD88 -/- mice to further investigate MyD88 involvement in iron sensing and hepcidin induction...
2018: Frontiers in Physiology
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