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Serotonin organoids

Vanja Dakic, Juliana Minardi Nascimento, Rafaela Costa Sartore, Renata de Moraes Maciel, Draulio B de Araujo, Sidarta Ribeiro, Daniel Martins-de-Souza, Stevens K Rehen
Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate experimental models have limited the understanding of how such substances impact human brain metabolism. Here we used shotgun mass spectrometry to explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on human cerebral organoids...
October 9, 2017: Scientific Reports
Nicholas W Bellono, James R Bayrer, Duncan B Leitch, Joel Castro, Chuchu Zhang, Tracey A O'Donnell, Stuart M Brierley, Holly A Ingraham, David Julius
Dietary, microbial, and inflammatory factors modulate the gut-brain axis and influence physiological processes ranging from metabolism to cognition. The gut epithelium is a principal site for detecting such agents, but precisely how it communicates with neural elements is poorly understood. Serotonergic enterochromaffin (EC) cells are proposed to fulfill this role by acting as chemosensors, but understanding how these rare and unique cell types transduce chemosensory information to the nervous system has been hampered by their paucity and inaccessibility to single-cell measurements...
June 29, 2017: Cell
Nikkie van der Wielen, Jean Paul Ten Klooster, Susanne Muckenschnabl, Raymond Pieters, Henk Fj Hendriks, Renger F Witkamp, Jocelijn Meijerink
BACKGROUND: Glucagon-like peptide 1 (GLP-1) contributes to satiety and plays a pivotal role in insulin secretion and glucose homeostasis. Similar to GLP-1, peptide YY (PYY) and cholecystokinin also influence food intake. The secretion of these hormones by enteroendocrine cells along the intestine is modulated by nutrients. Preparations from the Stevia rebaudiana plant, including rebaudioside A, are increasingly being used as noncaloric sweeteners. OBJECTIVE: We investigated the effects of rebaudioside A on enteroendocrine cells by assessing both cell numbers as well as their secretory capacity in an organoid model...
December 2016: Journal of Nutrition
Takeshi Tsuruta, Shinichi Saito, Yosuke Osaki, Akihiro Hamada, Ayako Aoki-Yoshida, Kei Sonoyama
Intestinal organoids were recently established as an ex vivo model of the intestinal epithelium. The present study investigated the serotonin (5-hydroxytryptamine, 5-HT) system using organoids. Organoids from murine small intestinal and colonic crypts were successfully cultured. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that small intestinal and colonic organoids express mRNAs encoding tryptophan hydroxylase-1 (TPH1) (the rate-limiting enzyme of 5-HT synthesis), serotonin reuptake transporter (SERT), 5-HT receptor (HTR)2A, HTR2B, and HTR4...
May 20, 2016: Biochemical and Biophysical Research Communications
Ryotaro Bouchi, Kylie S Foo, Haiqing Hua, Kyoichiro Tsuchiya, Yoshiaki Ohmura, P Rodrigo Sandoval, Lloyd E Ratner, Dieter Egli, Rudolph L Leibel, Domenico Accili
Generation of surrogate sources of insulin-producing β-cells remains a goal of diabetes therapy. While most efforts have been directed at differentiating embryonic or induced pluripotent stem (iPS) cells into β-like-cells through endodermal progenitors, we have shown that gut endocrine progenitor cells of mice can be differentiated into glucose-responsive, insulin-producing cells by ablation of transcription factor Foxo1. Here we show that FOXO1 is present in human gut endocrine progenitor and serotonin-producing cells...
June 30, 2014: Nature Communications
Mouna Mnif Feki, Fatma Mnif, Mahdi Kamoun, Nadia Charfi, Nabila Rekik, Basma Ben Naceur, Lilia Mnif, Tahia Sellami Boudawara, Mohamed Abid
Acromegaly is usually the result of a pituitary growth hormone cell-adenoma or is more rarely due to ectopic secretion of growth hormone releasing hormone (GHRH).We report the case of a 60-year-old woman with acromegaly due to a GH-RH-secreting pancreatic tumor. Laboratory evaluation confirmed the diagnosis of acromegaly. Magnetic resonance imaging revealed a partial empty sella with no signs of adenoma. Ultrasound sonography performed for abdominal pains showed a calcified large heterogeneous infrahepatic mass...
December 2011: Annales D'endocrinologie
Boon Hwa Neo, Sharath Kandhi, Michael S Wolin
We have previously provided evidence that hydrogen peroxide (H(2)O(2)) stimulates soluble guanylate cyclase (sGC) under conditions where it relaxes isolated endothelium-removed bovine pulmonary arteries (BPAs). Since it was recently reported that H(2)O(2) induces coronary vasorelaxation associated with a nitric oxide/cGMP-independent thiol oxidation/subunit dimerization-elicited activation of protein kinase G (PKG), we investigated whether this mechanism participates in the relaxation of BPAs to H(2)O(2). BPAs precontracted with serotonin (incubated under hypoxia to lower endogenous H(2)O(2)) were exposed to increasing concentrations of H(2)O(2)...
October 2010: American Journal of Physiology. Heart and Circulatory Physiology
Janet L Cunningham, Lars Grimelius, Anders Sundin, Smriti Agarwal, Eva T Janson
Patients with malignant serotonin-producing carcinoid tumours in the jejunum, ileum and caecum generally have long survival expectancy. In some patients, however, tumour progression is more rapid and there is a need to identify them at an early stage. The purpose of this study was to determine if histopathological characteristics and/or Ki67 and apoptotic indices are of prognostic value in cases of metastatic disease. Eighty-one patients with this tumour were included in the study; all had metastases and their survival range was 1-223 months...
2007: Acta Oncologica
Christopher J Mingone, Sachin A Gupte, Joseph L Chow, Mansoor Ahmad, Nader G Abraham, Michael S Wolin
Protoporphyrin IX is an activator of soluble guanylate cyclase (sGC), but its role as an endogenous regulator of vascular function through cGMP has not been previously reported. In this study we examined whether the heme precursor delta-aminolevulinic acid (ALA) could regulate vascular force through promoting protoporphyrin IX-elicited activation of sGC. Exposure of endothelium-denuded bovine pulmonary arteries (BPA) in organoid culture to increasing concentrations of the heme precursor ALA caused a concentration-dependent increase in BPA epifluorescence, consistent with increased tissue protoporphyrin IX levels, associated with decreased force generation to increasing concentrations of serotonin...
September 2006: American Journal of Physiology. Lung Cellular and Molecular Physiology
Christelle Guibert, Jean Pierre Savineau, Huguette Crevel, Roger Marthan, Eric Rousseau
1 Organoid cultured explants from differentiated tissues have gained renewed interest in the undertaking of physiological and pharmacological studies. In the work herein, we examined the pharmaco-mechanical properties of an in vitro model consisting of organoid cultured rings derived from rat extra- and intrapulmonary arteries, over a period of 4 days in culture. 2 Mechanical changes were quantified using isometric tension measurements on both fresh and cultured pulmonary arterial tissues, with experiments performed in the presence or absence of 10% foetal calf serum...
November 2005: British Journal of Pharmacology
Jie Pan, Herman Yeger, Ernest Cutz
We investigated the development of innervation of the pulmonary neuroendocrine cell (PNEC) system composed of single cells and organoid cell clusters, neuroepithelial bodies (NEB) in rabbit fetal and neonatal lungs. To visualize the nerve fibers and their contacts with PNECs/NEBs, we used confocal microscopy and multilabel immunohistochemistry (IHC) with pan-neural marker, synaptic vesicle protein 2 (SV2), and serotonin (5-HT) as markers for PNECs/NEBs, and smooth muscle actin or cytokeratin to identify airway landmarks...
March 2004: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
No abstract text is available yet for this article.
January 1965: Life Sciences (1962)
Erik N T P Bakker, Esther T van der Meulen, Bernard M van den Berg, Vincent Everts, Jos A E Spaan, Ed VanBavel
The hypothesis was tested that chronic vasoconstriction is followed by a structural reduction in lumen diameter, measured at full dilation. An in vitro model of pressurized rat skeletal muscle arterioles was used. During a 3-day experimental period, constriction of active vessels was achieved with fetal calf serum or endothelin-1 (ET-1). Maximal dilation revealed inward remodeling from 179 +/- 6.5 microm lumen diameter on day 0 to 151 +/- 6.3 microm on day 3 at 75 mm Hg in vessels incubated with serum (n = 8)...
January 2002: Journal of Vascular Research
E N Bakker, E T van Der Meulen, J A Spaan, E VanBavel
We developed an organoid culture technique to study the mechanisms involved in arterial remodeling. Resistance arteries were isolated from rat cremaster muscle and mounted in a pressure myograph at 75 mmHg. Vessels were studied during a 4-day culture period in DMEM with either 2% albumin, 10% heat-inactivated FCS (HI-FCS) or 10% dialyzed HI-FCS (12 kDa cut off) added to the perfusate. The albumin group showed a gradual loss of endothelial function and integrity, whereas smooth muscle agonist and myogenic responses were retained...
April 2000: American Journal of Physiology. Heart and Circulatory Physiology
Y Kikuchi
The respiratory tract epithelium in many vertebrate species has a neuroepithelial endocrine (NEE) system. This system is composed of solitary NEE cells or organoid cell groups called neuroepithelial bodies (NEBs). In response to chemical and physical stimuli, NEE cells may release bioactive substances. Serotonin, one of the biogenic amines, well-known as a constricter of smooth muscle, can be found in NEE cells, serotonin-immunoreactive cells can be used as a marker for these cells. Comparative histological studies of lower vertebrates can improve our understanding of mammalian respiratory systems...
December 1995: Kaibogaku Zasshi. Journal of Anatomy
C Hagen-Aukamp, W Wesemann, G Aumüller
Megakaryocytes were isolated from rat and guinea pig bone marrow by Percoll density and/or elutriator centrifugation. As compared with the suspension of bone marrow an enrichment of factor 530 with a recovery of 34% can be achieved if a combination of both methods is applied.--The pattern of DNA percentage distribution in isolated cells paralleled the polyploidy found in megakaryocytes of the bone marrow. As determined by scanning cytophotometry and impulse cytophotometry the percentage distribution was maximal for the 16 C cells...
December 1980: European Journal of Cell Biology
G Jaim-Etcheverry, L M Zieher
No abstract text is available yet for this article.
1980: Cell and Tissue Research
L Wiklund, L Descarries, K Møllgård
No abstract text is available yet for this article.
December 1981: Journal of Neurocytology
M T Juillard, J P Collin
In the pineal gland of the mouse the distribution of serotonin (5-HT) and its eventual relationships to a protein secretion were examined by means of fluorescence histochemical (Falck-Hillarp) and ultracytochemical (chromaffin and argentaffin) methods. (1) Yellow formaldehyde-induced fluorophores, characteristic of high concentrations of 5-HT, were found in pinealocytes, interstitial cells and sympathetic adrenergic nerve endings. The 5-HT content was studied according to the circadian variations and different drug treatments...
1980: Cell and Tissue Research
P Voison
An electron microscopic study of the pineal organ of the pigeon was conducted under natural conditions and after short in vitro incubation or culture. Two cell types could be identified in the epithelium: receptor line cells (RLC) and interstitial cells of the ependymal type. Good preservation of the cells' fine structure could be observed whatever the different incubation times used. RLC display cytological features reflecting the existence of a protein secretory process and of regression of the photoreceptor structures (secretory rudimentary photoreceptor cells)...
1982: Archives D'anatomie Microscopique et de Morphologie Expérimentale
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