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https://www.readbyqxmd.com/read/28102348/ho-1-inhibits-preadipocyte-proliferation-and-differentiation-at-the-onset-of-obesity-via-ros-dependent-activation-of-akt2
#1
Gabriel Wagner, Josefine Lindroos-Christensen, Elisa Einwallner, Julia Husa, Thea-Christin Zapf, Katharina Lipp, Sabine Rauscher, Marion Gröger, Andreas Spittler, Robert Loewe, Florian Gruber, J Catharina Duvigneau, Thomas Mohr, Hedwig Sutterlüty-Fall, Florian Klinglmüller, Gerhard Prager, Berthold Huppertz, Jeanho Yun, Oswald Wagner, Harald Esterbauer, Martin Bilban
Excessive accumulation of white adipose tissue (WAT) is a hallmark of obesity. The expansion of WAT in obesity involves proliferation and differentiation of adipose precursors, however, the underlying molecular mechanisms remain unclear. Here, we used an unbiased transcriptomics approach to identify the earliest molecular underpinnings occuring in adipose precursors following a brief HFD in mice. Our analysis identifies Heme Oxygenase-1 (HO-1) as strongly and selectively being upregulated in the adipose precursor fraction of WAT, upon high-fat diet (HFD) feeding...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28102342/neural-activity-in-the-dorsal-medial-superior-temporal-area-of-monkeys-represents-retinal-error-during-adaptive-motor-learning
#2
Aya Takemura, Tomoyo Ofuji, Kenichiro Miura, Kenji Kawano
To adapt to variable environments, humans regulate their behavior by modulating gains in sensory-to-motor processing. In this study, we measured a simple eye movement, the ocular following response (OFR), in monkeys to study the neuronal basis of adaptive motor learning in the visuomotor processing stream. The medial superior temporal (MST) area of the cerebral cortex is a critical site for contextual gain modulation of the OFR. However, the role of MST neurons in adaptive gain modulation of the OFR remains unknown...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28102227/singular-location-and-signaling-profile-of-adenosine-a2a-cannabinoid-cb1-receptor-heteromers-in-the-dorsal-striatum
#3
Estefanía Moreno, Anna Chiarlone, Mireia Medrano, Mar Puigdellívol, Lucka Bibic, Lesley A Howell, Eva Resel, Nagore Puente, María J Casarejos, Juan Perucho, Joaquín Botta, Nuria Suelves, Francisco Ciruela, Silvia Ginés, Ismael Galve-Roperh, Vicent Casadó, Pedro Grandes, Beat Lutz, Krisztina Monory, Enric I Canela, Carmen Lluís, Peter J McCormick, Manuel Guzmán
The dorsal striatum is a key node for many neurobiological processes such as motor activity, cognitive functions, and affective processes. The proper functioning of striatal neurons relies critically on metabotropic receptors. Specifically, the main adenosine and endocannabinoid receptors present in the striatum, ie, adenosine A2A receptor (A2AR) and cannabinoid CB1 receptor (CB1R), are of pivotal importance in the control of neuronal excitability. Facilitatory and inhibitory functional interactions between striatal A2AR and CB1R have been reported, and evidence supports that this cross-talk may rely, at least in part, on the formation of A2AR-CB1R heteromeric complexes...
January 19, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28101181/microrna-351-inhibits-denervation-induced-muscle-atrophy-by-targeting-traf6
#4
Qianru He, Jiaying Qiu, Ming Dai, Qingqing Fang, Xiaoqing Sun, Yanpei Gong, Fei Ding, Hualin Sun
MicroRNAs (miRs) have been observed to be involved in the modulation of various physiopathological processes. However, the impacts of miRNAs on muscle atrophy have not been fully investigated. In the present study, the results demonstrated that miR-351 was differentially expressed in the tibialis anterior (TA) muscle at various times following sciatic nerve transection, and the time-dependent expression profile of miR-351 was inversely correlated with that of tumor necrosis factor receptor-associated factor 6 (TRAF6) at the mRNA and protein levels...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28100826/biomechanical-properties-of-wheat-grains-the-implications-on-milling
#5
James E Hourston, Michael Ignatz, Martin Reith, Gerhard Leubner-Metzger, Tina Steinbrecher
Millennia of continuous innovation have driven ever increasing efficiency in the milling process. Mechanically characterizing wheat grains and discerning the structure and function of the wheat bran layers can contribute to continuing innovation. We present novel shear force and puncture force testing regimes to characterize different wheat grain cultivars. The forces endured by wheat grains during the milling process can be quantified, enabling us to measure the impact of commonly applied grain pretreatments, such as microwave heating, extended tempering, enzyme and hormone treatments on grains of different 'hardness'...
January 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28100751/decision-related-activity-in-macaque-v2-for-fine-disparity-discrimination-is-not-compatible-with-optimal-linear-readout
#6
Stephane Clery, Bruce G Cumming, Hendrikje Nienborg
: Fine judgments of stereoscopic depth rely mainly on relative judgments of depth (relative binocular disparity) between objects, rather than judgments of the distance to where the eyes are fixating (absolute disparity). In macaques, visual area V2 is the earliest site in the visual processing hierarchy for which neurons selective for relative disparity have been observed (Thomas et al., 2002). Here, we found that, in macaques trained to perform a fine disparity discrimination task, disparity-selective neurons in V2 were highly selective for the task, and their activity correlated with the animals' perceptual decisions (unexplained by the stimulus)...
January 18, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28100734/does-the-superior-colliculus-control-perceptual-sensitivity-or-choice-bias-during-attention-evidence-from-a-multialternative-decision-framework
#7
Devarajan Sridharan, Nicholas A Steinmetz, Tirin Moore, Eric I Knudsen
: Distinct networks in the forebrain and the midbrain coordinate to control spatial attention. The critical involvement of the superior colliculus (SC)-the central structure in the midbrain network-in visuospatial attention has been shown by four seminal, published studies in monkeys (Macaca mulatta) performing multialternative tasks. However, due to the lack of a mechanistic framework for interpreting behavioral data in such tasks, the nature of the SC's contribution to attention remains unclear...
January 18, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28100501/mdm2-mediates-fibroblast-activation-and-renal-tubulointerstitial-fibrosis-via-p53-independent-pathway
#8
Chen Ye, Hui Tang, Zheng Zhao, Chun-Tao Lei, Chao-Qun You, Jiao Zhang, Pan Gao, Fang-Fang He, Shan Chen, Yumei Wang, Chun Zhang, Hua Su
It is well-recognized that Murine Double Minute gene 2 (MDM2) plays a critical role in cell proliferation and inflammatory processes during tumorigenesis. Also it is reported that MDM2 is expressed in glomeruli and involved in podocyte injury. However, whether MDM2 is implicated in renal fibrosis remains unclear. Here we investigated the role of MDM2 in tubulointerstitial fibrosis (TIF). By immunohistochemical staining and western blotting we confirmed that MDM2 is up-regulated in tubulointerstitial compartment in TIF patients and Unilateral Urethral Obstruction (UUO) mice, which mainly originates from myofibroblasts...
January 18, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28098858/mir-345-inhibits-tumor-metastasis-and-emt-by-targeting-irf1-mediated-mtor-stat3-akt-pathway-in-hepatocellular-carcinoma
#9
Miao Yu, Huanzhou Xue, Yadong Wang, Quan Shen, Qingfeng Jiang, Xiao Zhang, Ke Li, Meng Jia, Jiangkun Jia, Jian Xu, Yuwei Tian
MicroRNAs (miRNAs) have been reported to play critical roles in tumor progression including hepatocellular carcinoma (HCC). Thus, the underlying mechanisms need further investigation. Previous study reported that loss of miR-345 expression indicated a poor prognosis of HCC patients. This study evaluated whether loss of miR-345 could promote the tumor metastasis and epithelial-mesenchymal-transition (EMT) of HCC by targeting interferon regulatory factor 1 (IRF1)-mediated mTOR/STAT3/AKT signaling. Underexpression of miR-345 was identified in 65 cases of human HCC compared to matched tumor-adjacent tissues by qRT-PCR...
January 17, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28098802/penduliflaworosin-a-diterpenoid-from-croton-crassifolius-exerts-anti-angiogenic-effect-via-vegf-receptor-2-signaling-pathway
#10
Yeyin Liang, Yubo Zhang, Guocai Wang, Yaolan Li, Weihuan Huang
Anti-angiogenesis targeting vascular endothelial growth factor receptor-2 (VEGFR-2) has been considered as an important strategy for cancer therapy. Penduliflaworosin is a diterpenoid isolated from the plant Croton crassifolius. Our previous study showed that this diterpenoid possesses strong anti-angiogenic activity by inhibiting vessel formation in zebrafish. This study was conducted to further investigate the anti-angiogenic activity and mechanism of penduliflaworosin. Results revealed that penduliflaworosin significantly inhibited VEGF-induced angiogenesis processes including proliferation, invasion, migration, and tube formation of human umbilical vein endothelial cells (HUVECs)...
January 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28096203/hospital-variation-in-functional-recovery-after-stroke
#11
Janet Prvu Bettger, Laine Thomas, Li Liang, Ying Xian, Cheryl D Bushnell, Jeffrey L Saver, Gregg C Fonarow, Eric D Peterson
BACKGROUND: Functional status is a key patient-centric outcome, but there are little data on whether functional recovery post-stroke varies among hospitals. This study examined the distribution of functional status 3 months after stroke, determined whether these outcomes vary among hospitals, and identified hospital characteristics associated with better (or worse) functional outcomes. METHODS AND RESULTS: Observational analysis of the AVAIL study (Adherence Evaluation After Ischemic Stroke-Longitudinal) included 2083 ischemic stroke patients enrolled from 82 US hospitals participating in Get With The Guidelines-Stroke and AVAIL...
January 2017: Circulation. Cardiovascular Quality and Outcomes
https://www.readbyqxmd.com/read/28095922/long-noncoding-rna-braveheart-promotes-cardiogenic-differentiation-of-mesenchymal-stem-cells-in-vitro
#12
Jingying Hou, Huibao Long, Changqing Zhou, Shaoxin Zheng, Hao Wu, Tianzhu Guo, Quanhua Wu, Tingting Zhong, Tong Wang
BACKGROUND: Mesenchymal stem cells (MSCs) have limited potential of cardiogenic differentiation. In this study, we investigated the influence of long noncoding RNA Braveheart (lncRNA-Bvht) on cardiogenic differentiation of MSCs in vitro. METHODS: MSCs were obtained from C57BL/6 mice and cultured in vitro. Cells were divided into three groups: blank control, null vector control, and lncRNA-Bvht. All three groups experienced exposure to hypoxia (1% O2) and serum deprivation for 24 h, and 24 h of reoxygenation (20% O2)...
January 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28095661/molecular-cloning-and-characterization-of-a-p38-like-mitogen-activated-protein-kinase-from-echinococcus-granulosus
#13
Guodong Lü, Jing Li, Chuanshan Zhang, Liang Li, Xiaojuan Bi, Chaowang Li, Jinliang Fan, Xiaomei Lu, Dominique A Vuitton, Hao Wen, Renyong Lin
Cystic echinococcosis (CE) treatment urgently requires a novel drug. The p38 mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases, but still have to be characterized in Echinococcus granulosus. We identified a 1,107 bp cDNA encoding a 368 amino acid MAPK protein (Egp38) in E. granulosus. Egp38 exhibits 2 distinguishing features of p38-like kinases: a highly conserved T-X-Y motif and an activation loop segment. Structural homology modeling indicated a conserved structure among Egp38, EmMPK2, and H...
December 2016: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28095496/opportunities-for-integrated-ecological-analysis-across-inland-australia-with-standardised-data-from-ausplots-rangelands
#14
Greg R Guerin, Ben Sparrow, Andrew Tokmakoff, Anita Smyth, Emrys Leitch, Zdravko Baruch, Andrew J Lowe
Australian rangelands ecosystems cover 81% of the continent but are understudied and continental-scale research has been limited in part by a lack of precise data that are standardised between jurisdictions. We present a new dataset from AusPlots Rangelands that enables integrative rangelands analysis due to its geographic scope and standardised methodology. The method provides data on vegetation and soils, enabling comparison of a suite of metrics including fractional vegetation cover, basal area, and species richness, diversity, and composition...
2017: PloS One
https://www.readbyqxmd.com/read/28095466/substrate-stiffness-controls-osteoblastic-and-chondrocytic-differentiation-of-mesenchymal-stem-cells-without-exogenous-stimuli
#15
Rene Olivares-Navarrete, Erin M Lee, Kathryn Smith, Sharon L Hyzy, Maryam Doroudi, Joseph K Williams, Ken Gall, Barbara D Boyan, Zvi Schwartz
Stem cell fate has been linked to the mechanical properties of their underlying substrate, affecting mechanoreceptors and ultimately leading to downstream biological response. Studies have used polymers to mimic the stiffness of extracellular matrix as well as of individual tissues and shown mesenchymal stem cells (MSCs) could be directed along specific lineages. In this study, we examined the role of stiffness in MSC differentiation to two closely related cell phenotypes: osteoblast and chondrocyte. We prepared four methyl acrylate/methyl methacrylate (MA/MMA) polymer surfaces with elastic moduli ranging from 0...
2017: PloS One
https://www.readbyqxmd.com/read/28093474/behavioral-status-influences-the-dependence-of-odorant-induced-change-in-firing-on-pre-stimulus-firing-rate
#16
Anan Li, Ethan M Guthman, Wilder T Doucette, Diego Restrepo
: The firing rate of the mitral/tufted cells in the olfactory bulb is known to undergo significant trial-to-trial variability and is affected by anesthesia. Here we ask whether odorant-elicited changes in firing rate depend on the rate before application of the stimulus in the awake and anesthetized mouse. We find that pre-stimulus firing rate varies widely on a trial-to-trial basis and that the stimulus-induced change in firing rate decreases with increasing pre-stimulus firing rate...
January 16, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28092011/microbubble-moving-in-blood-flow-in-microchannels-effect-on-the-cell-free-layer-and-cell-local-concentration
#17
David Bento, Lúcia Sousa, Tomoko Yaginuma, Valdemar Garcia, Rui Lima, João M Miranda
Gas embolisms can hinder blood flow and lead to occlusion of the vessels and ischemia. Bubbles in microvessels circulate as tubular bubbles (Taylor bubbles) and can be trapped, blocking the normal flow of blood. To understand how Taylor bubbles flow in microcirculation, in particular, how bubbles disturb the blood flow at the scale of blood cells, experiments were performed in microchannels at a low Capillary number. Bubbles moving with a stream of in vitro blood were filmed with the help of a high-speed camera...
March 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28091791/a-novel-scalable-robust-downstream-process-for-oncolytic-rat-parvovirus-isoelectric-point-based-elimination-of-empty-particles
#18
Barbara Leuchs, Veronika Frehtman, Markus Riese, Marcus Müller, Jean Rommelaere
The rodent protoparvovirus H-1PV, with its oncolytic and oncosuppressive properties, is a promising anticancer agent currently under testing in clinical trials. This explains the current demand for a scalable, good manufacturing practice-compatible virus purification process yielding high-grade pure infectious particles and overcoming the limitations of the current system based on density gradient centrifugation. We describe here a scalable process offering high purity and recovery. Taking advantage of the isoelectric point difference between full and empty particles, it eliminates most empty particles...
January 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28089962/mycoplasma-clearance-and-risk-analysis-in-a-model-bioprocess
#19
Julie Wang, Sarah Johnson, Matthew Brown, Scott Lute, Cyrus Agarabi, Alena Dabrazhynetskaya, Vladimir Chizhikov, Kurt Brorson
Mycoplasmas are a type of bacteria that lack cell walls and are occasional cell culture contaminants. In a biotechnology setting, because they can pass through 0.2 μm filters, mycoplasmas could pose a potential patient safety hazard if undetected contaminants from the production culture were not completely removed by downstream biotechnology manufacturing. In this study we investigated the ability of typical commercial monoclonal antibody (mAb) purification operations to clear and kill mycoplasmas, using Acholeplasma laidlawii as a model organism...
January 15, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28089560/critical-role-of-p2x7-receptors-in-the-neuroinflammation-and-cognitive-dysfunction-after-surgery
#20
Bin Zheng, Renchun Lai, Jun Li, Zhiyi Zuo
Postoperative cognitive dysfunction worsens patient outcome after surgery. Neuroinflammation is a critical neuropathological process for it. We determined the role of P2X7 receptors, proteins that participate in inflammatory response, in the neuroinflammation induction after surgery, and whether the choice of volatile anesthetics affects its occurrence. Eight-week old C57BL/6J or P2X7 receptor knockout male mice were subjected to right carotid arterial exposure under anesthesia with 1.8% isoflurane, 2.5% sevoflurane or 10% desflurane...
January 9, 2017: Brain, Behavior, and Immunity
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