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https://www.readbyqxmd.com/read/28448441/characterization-of-compounds-with-tumor-cell-proliferation-inhibition-activity-from-mushroom-phellinus-baumii-mycelia-produced-by-solid-state-fermentation
#1
Henan Zhang, Qian Shao, Wenhan Wang, Jingsong Zhang, Zhong Zhang, Yanfang Liu, Yan Yang
The inhibition of tumor-cell proliferationbyan organicsolvent extract from the solid-state fermentation of Phellinus baumii mycelia inoculated in rice medium was investigated in vitro. The active compounds inhibiting tumor-cell proliferation were characterized. Results revealed that all (petroleum ether, chloroform, ethyl acetate, and butanol) fractions inhibited tumor-cell proliferation in a dose-dependent fashion. The ethyl acetate extract had the highest inhibitory effecton tumor-cell proliferation, and the butanol fraction had the lowest...
April 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28447046/genetic-diversity-analysis-of-morchella-sp-by-rapd
#2
Muhammad Irfan, Shuang Yang, Luo Yuxin, Jia-Xing Sun
This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymorphism in three species of morchella. Out of all eight primers tested in current study, one of them (B8) resulted 100% polymorphism among the three studied species...
March 2017: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28445613/neuronal-distribution-of-tyramine-and-the-tyramine-receptor-amtar1-in-the-honeybee-brain
#3
Markus Thamm, Christina Scholl, Tina Reim, Kornelia Grübel, Karin Möller, Wolfgang Rössler, Ricarda Scheiner
Tyramine is an important neurotransmitter, neuromodulator, and neurohormone in insects. In honeybees, it is assumed to have functions in modulating sensory responsiveness and controlling motor behavior. Tyramine can bind to two characterized receptors in honeybees, both of which are coupled to intracellular cAMP pathways. How tyramine acts on neuronal, cellular and circuit levels is unclear. We investigated the spatial brain expression of the tyramine receptor AmTAR1 using a specific antibody. This antibody detects a membrane protein of the expected molecular weight in Western blot analysis...
April 26, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28445513/dietary-patterns-and-all-cause-cancer-and-cardiovascular-disease-mortality-in-japanese-men-and-women-the-japan-public-health-center-based-prospective-study
#4
Akiko Nanri, Tetsuya Mizoue, Taichi Shimazu, Junko Ishihara, Ribeka Takachi, Mitsuhiko Noda, Hiroyasu Iso, Shizuka Sasazuki, Norie Sawada, Shoichiro Tsugane
OBJECTIVE: A meta-analysis showed an inverse association of a prudent/healthy dietary pattern with all-cause mortality and no association of a western/unhealthy dietary pattern. However, the association of distinctive dietary patterns of Japanese population with mortality remains unclear. We prospectively investigated the association between dietary patterns and all-cause, cancer, and cardiovascular disease mortality among Japanese adults. METHODS: Participants were 36,737 men and 44,983 women aged 45-74 years who participated in the second survey of the Japan Public Health Center-based Prospective Study (1995-1998) and who had no history of serious disease...
2017: PloS One
https://www.readbyqxmd.com/read/28444567/the-behavior-of-antibiotic-resistance-genes-and-arsenic-influenced-by-biochar-during-different-manure-composting
#5
Erping Cui, Ying Wu, Yanan Jiao, Yiru Zuo, Christopher Rensing, Hong Chen
The effect of two different biochar types, rice straw biochar (RSB) and mushroom biochar (MB), on chicken manure composting was previously examined by monitoring the fate of antibiotic resistance genes (ARGs) and arsenic. The behavior of ARGs and arsenic in other kinds of manure composting with the same biochar types had not been examined. In this study, we added either RSB or MB to pig and duck manure composts to study the behavior of ARGs (tet genes, sul genes, and chloramphenicol resistance genes) and arsenic under the same experimental condition...
April 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28442576/determination-of-dendritic-spine-morphology-by-the-striatin-scaffold-protein-strn4-through-interaction-with-the-phosphatase-pp2a
#6
Lianfeng Lin, Louisa Hoi-Ying Lo, Quanwei Lyu, Kwok-On Lai
Dendritic spines are heterogeneous and exist with various morphologies. Altered spine morphology might underlie the cognitive deficits in neurodevelopmental disorders such as autism, but how different subtypes of dendritic spines are selectively maintained along development is still poorly understood. Spine maturation requires spontaneous activity of N-methyl-D-aspartate (NMDA) receptor and local dendritic protein synthesis. STRN4 (also called Zinedin) belongs to the striatin family of scaffold proteins, and some of the potential striatin-interacting proteins are encoded by autism risk genes...
April 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28441464/crucial-roles-of-pox-neuro-in-the-developing-ellipsoid-body-and-antennal-lobes-of-the-drosophila-brain
#7
Shilpi Minocha, Werner Boll, Markus Noll
The paired box gene Pox neuro (Poxn) is expressed in two bilaterally symmetric neuronal clusters of the developing adult Drosophila brain, a protocerebral dorsal cluster (DC) and a deutocerebral ventral cluster (VC). We show that all cells that express Poxn in the developing brain are postmitotic neurons. During embryogenesis, the DC and VC consist of only 20 and 12 neurons that express Poxn, designated embryonic Poxn-neurons. The number of Poxn-neurons increases only during the third larval instar, when the DC and VC increase dramatically to about 242 and 109 Poxn-neurons, respectively, virtually all of which survive to the adult stage, while no new Poxn-neurons are added during metamorphosis...
2017: PloS One
https://www.readbyqxmd.com/read/28440038/induced-chemical-defense-of-a-mushroom-by-a-double-bond-shifting-polyene-synthase
#8
Philip Brandt, María García-Altares, Markus Nett, Christian Hertweck, Dirk Hoffmeister
The antilarval mushroom polyenes 18-methyl-19-oxoicosaoctaenoic acid and 20-methyl-21-oxodocosanonaenoic acid appear in response to injury of the mycelium of the stereaceous mushroom BY1. We identified a polyketide synthase (PPS1) which belongs to a hitherto completely uncharacterized clade of polyketide synthases. Expression of the PPS1 gene is massively upregulated following mycelial damage. The synthesis of the above polyenes was reconstituted in the mold Aspergillus niger as a heterologous host. This demonstrates that PPS1 1) synchronously produces branched-chain polyketides of varied lengths, and 2) catalyzes the unprecedented shift of eight or nine double bonds...
April 25, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28438481/genes-and-neural-circuits-for-sleep-of-the-fruit-fly
#9
REVIEW
Jun Tomita, Gosuke Ban, Kazuhiko Kume
Sleep is a universal physiological state evolutionarily conserved among species, but the molecular basis for its regulation is still largely unknown. Due to its electroencephalogram criteria, sleep has long been investigated and described mostly in mammalian species. The fruit fly, Drosophila melanogaster, has emerged as a genetic model organism for studying sleep. The Drosophila sleep is behaviorally defined, and is tightly regulated by circadian and homeostatic processes, like mammals. Genetic analyses using Drosophila have successfully identified a number of conserved regulatory mechanisms underlying sleep between flies and mammals...
April 21, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28438013/characterization-of-newly-bred-cordyceps-militaris-strains-for-higher-production-of-cordycepin-through-hplc-and-urp-pcr-analysis
#10
Hyun-Hee Lee, Naru Kang, Inmyoung Park, Jungwook Park, Inyoung Kim, Jieun Kim, Namgyu Kim, Jae-Yun Lee, Young-Su Seo
Cordyceps militaris, a member of Ascomycota, a mushroom referred to as caterpillar Dong-chung-ha-cho, is commercially valuable because of its high content of bioactive substances including cordycepin and its potential for artificial cultivation. Cordycepin (3'-deoxyadenosine) is highly associated with the pharmacological effects of C. militaris. C. militaris is heterothallic in that two mating type loci, idiomorph MAT1-1 and MAT1-2, exist discretely in two different spores. In this study, nine C. militaris strains were mated with each other to prepare new strains that produced a larger amount of cordycepin than the parent strains...
April 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28437454/age-associated-increase-of-the-active-zone-protein-bruchpilot-within-the-honeybee-mushroom-body
#11
Katrin B Gehring, Karin Heufelder, Harald Depner, Isabella Kersting, Stephan J Sigrist, Dorothea Eisenhardt
In honeybees, age-associated structural modifications can be observed in the mushroom bodies. Prominent examples are the synaptic complexes (microglomeruli, MG) in the mushroom body calyces, which were shown to alter their size and density with age. It is not known whether the amount of intracellular synaptic proteins in the MG is altered as well. The presynaptic protein Bruchpilot (BRP) is localized at active zones and is involved in regulating the probability of neurotransmitter release in the fruit fly, Drosophila melanogaster...
2017: PloS One
https://www.readbyqxmd.com/read/28436508/purification-characterization-and-bioactivities-of-polysaccharides-from-pleurotus-ferulae
#12
Jinyu Li, Pengfei Yuan, Xinhui Wang, Adila Aipire, Minjing Li, Jie Yang, Hong Tao, Tianlei Ying, Changshuang Fu, Xianxian Wei, Fuchun Zhang, Jinyao Li
Pleurotus ferulae is an edible mushroom and has been used in Uygur medicine for a long time. In this study, we purified polysaccharides from P. ferulae (PFPS) and investigated its structural characteristics. We obtained a homogeneous PFPS with a molecular weight of around 1600 kDa and prominent characteristic polysaccharide groups, which mainly contained glucose (97%), followed by mannose and galactose (3%). Both (1)H and (13)C NMR spectra indicated that PFPS contained both α- and β-anomeric configurations...
April 24, 2017: Food & Function
https://www.readbyqxmd.com/read/28436327/mechanism-of-glucose-regulates-the-fruiting-body-formation-in-the-beech-culinary-medicinal-mushroom-hypsizygus-marmoreus-agaricomycetes
#13
Jin-Jing Zhang, Hui Chen, Min-Ying Xie, Ming-Jie Chen, Hai-Bo Hao, Hong Wang, Zhi-Yong Feng
To understand the fruiting process of Hypsizygus marmoreus, a synthetic liquid medium (SLM) was optimized to induce fruiting body initiation. Dependent on the SLM, the effect of a monofactor (glucose) on the fruiting bodies of H. marmoreus was studied at different concentrations (10 and 40 g/L). Primordia appeared approximately 10 days earlier in low-glucose media (LGM) than in high-glucose media (HGM), whereas mature fruiting bodies formed on mushrooms approximately 7 days earlier and more primordia developed into mature fruiting bodies when cultured in HGM...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436326/evaluation-and-characterization-of-nutritive-properties-of-the-jelly-ear-culinary-medicinal-mushroom-auricularia-auricula-judae-agaricomycetes-from-nigeria
#14
Reginald Chibueze Ohiri, Essien Eka Bassey
This study investigated the nutritive properties of the culinary-medicinal mushroom Auricularia auricula- judae. From the analysis, among the major minerals, potassium was found in the highest concentration, with a value of 172.03 ± 8.62 mg/kg, whereas manganese had the highest concentration among trace elements, with a value of 1.66 ± 0.11 mg/kg. Glutamic acid was the amino acid detected in the largest amounts in this study, with a value of 10.09 ± 1.86 mg/kg. Although glutamine and asparagine were not detected in this mushroom, cysteine and methionine had the lowest concentrations, with values of 0...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436325/photoperiodic-responses-and-characterization-of-the-cmvvd-gene-encoding-a-blue-light-photoreceptor-from-the-medicinal-caterpillar-fungus-cordyceps-militaris-ascomycetes
#15
Xin Zhang, Xiaoming Dong, Xinhua Song, Fen Wang, Caihong Dong
Light is a necessary environmental factor for production of conidia and pigment, formation of stroma, and development of Cordyceps militaris, a well-known edible and medicinal mushroom. In this study, an obvious rhythm loop was observed in certain strains of C. militaris under conditions of alternating 12-hour intervals of dark and light. A possibly related gene, Cmvvd, the homologue of the blue-light photoreceptor of Neurospora crassa, was cloned from the genome of C. militaris. The protein CmVVD is predicted to be 203 amino acids in length and is characterized by the presence of a light, oxygen, or voltage domain...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436324/identification-of-a-new-fungal-pathogen-causing-white-villous-disease-on-the-fruiting-body-of-the-culinary-medicinal-mushroom-auricularia-auricula-judae-agaricomycetes-in-china
#16
Jie-Chi Zhang, Xiang-Hui Kong, Pi-Qi Zhang, Jia-Ning Liu, Yin-Peng Ma, Xiao-Dong Dai, Zeng-Hua Han, Qing-Fang Ma, Xiao-Yong Wang, Li-Ping Yu
Auricularia auricula-judae is an edible and medicinal fungus ranking fourth in production among the edible fungi cultivated worldwide. White villous disease is rampant in Northeast China; it infects the fruiting bodies of A. auricula-judae by forming a white mycelial layer on its ventral side. The disease not only causes an unacceptable morphological appearance and a poor-quality product, but it also significantly reduces the yield. In this study, based on fungal morphology, ribosomal DNA internal transcribed spacer sequences, identification of species-specific primers, and the pathogenicity of the mycelia and spores, 2 fungal pathogens were isolated and identified as Fusarium equiseti and F...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436323/new-germplasms-of-the-culinary-medicinal-button-mushroom-agaricus-bisporus-agaricomycetes-two-wild-strains-from-the-tibetan-plateau-china
#17
Bin-Bin Li, Si-Ping Jiang, Ai-Guo Xu, Phurbu Dorji, Wen-Jing Wang, Xiao-Liang Wang, Tie-Zheng Wei, Zu-Tang Zhang, Yi-Jian Yao
Agaricus bisporus is one of the most important commercially cultivated culinary-medicinal mushrooms worldwide. In China, most of the cultivated strains of the fungus were introduced from other countries and cultivated in the eastern provinces. In this study, 2 wild strains of A. bisporus, 2091 and 2094, isolated from fresh specimens collected from the Tibetan Plateau, were domesticated and cultivated alongside a commercial hybrid strain, As2796, in Lhasa, China, for comparison in order to provide new germplasms for cultivation...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436322/typification-and-characterization-of-trametes-multicolor-agaricomycetes-a-perspective-species-of-medicinal-mushrooms
#18
Ivan V Zmitrovich, Margarita A Bondartseva, Nadezhda V Psurtseva, Solomon P Wasser
Nomenclature revision and enlarged taxonomical descriptions are still needed for some well-known species whose interpretation is complicated by many nomenclature or taxonomical problems. The polyporoid fungus widely known as Trametes ochracea (= Coriolus zonatus) belongs to such a problematic group. At the same time, recent data show that this species, like its sister species T. versicolor, seems to be a perspective subject for fungal biotechnology and pharmacology. This article is devoted to stabilizing the nomenclature of the species in question via lectotypification and epitypification of Boletus multicolor...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436321/screening-of-bioactive-compounds-of-medicinal-mushrooms-collected-on-tunisian-territory
#19
Ayda Khadhri, Chedia Aouadhi, Samira Aschi-Smiti
This study is, to our knowledge, the first to investigate the pharmacological importance of wild Tunisian mushrooms. Ethanolic extracts of 5 Tunisian mushrooms-Phellinus torulosus, Fomes fomentarius, Trametes versicolor, Pisolithus albus, and Fomitopsis pinicola-were collected from the Kroumirie Region (North Tunisia). The dry basidomes of mushrooms were extracted using ethanol and evaluated for total polyphenol, flavonoid, flavonol, tannin, proanthocyanidin, and anthocyanin content. In addition, their antioxidant activities were determined using 3 assays (testing 2,2-diphenyl-1-picrylhydrazyl [DPPH] radical scavenging, the reducing power of iron, and the iron-chelating power)...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28436320/antibacterial-activity-of-desert-truffles-from-saudi-arabia-against-staphylococcus-aureus-and-pseudomonas-aeruginosa
#20
Domenico Schillaci, Maria Grazia Cusimano, Stella Maria Cascioferro, Vita Di Stefano, Vincenzo Arizza, Marco Chiaramonte, Luigi Inguglia, Abdulhakim Bawadekji, Salvatore Davino, Maria Letizia Gargano, Giuseppe Venturella
Medicinal mushrooms represent an unlimited source of polysaccharides with nutritional, antitumoral, antibacterial, and immune-stimulating properties. Traditional studies of epigeous higher Basidiomycetes have recently been joined by studies of hypogeous fungi and, in particular, of so-called desert truffles. With the aim to obtain novel agents against bacteria of clinical importance, we focused on the edible desert truffle mushrooms Tirmania pinoyi, Terfezia claveryi, and Picoa juniperi as sources of new antimicrobial agents...
2017: International Journal of Medicinal Mushrooms
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